CN210560374U - Low-cost anaerobic fermentation device capable of sampling for multiple times - Google Patents

Abstract

The utility model discloses a but low-cost anaerobic fermentation device of many times sample, its structure includes nitrogen cylinder, sample bottle, state monitoring bottle and the pipe-line system who corresponds. The nitrogen cylinder passes through the relief pressure valve and links to each other with first pipeline, the exit end of first pipeline is located the sample bottle bottom, is connected with the second pipeline that is used for the sample on the sample bottle, and its end is located the bottom of sample bottle, the sample bottle still communicates with the state monitoring bottle through the third pipeline mutually, the both ends of third pipeline are located the sample bottle respectively and the inside upper portion of state detection bottle, be provided with fourth pipeline and fifth pipeline on the state detection bottle, be provided with the pressure vacuum meter at the end of fourth pipeline. And the five pipelines are provided with necessary degerming air filters and valves. The device can avoid using expensive complicated anaerobic fermentation jar, and convenient, swift, low-cost realization satisfies the anaerobic culture under the control cultivation rotational speed, control and the monitoring dissolved oxygen, continuous sampling requirement.

Description

Low-cost anaerobic fermentation device capable of sampling for multiple times

Technical Field

The utility model relates to the field of chemical industry and medicine, especially a low-cost anaerobic fermentation device that can take a sample many times.

Background

At present, the common anaerobic fermentation devices on the market comprise anaerobic fermentation tanks, anaerobic workstations, small anaerobic tanks and the like. The prices of the fermentation tank and the anaerobic workstation are tens of thousands or even hundreds of thousands of prices which are too expensive for the early small test stage of a laboratory, and the anaerobic tank (needing to be matched with the anaerobic gas generating bag) is suitable for one-time culture without continuous sampling and can not sample the bacterial liquid for multiple times. That is, in research institutions such as biology laboratories, a small-sized anaerobic culture device which is low in cost, convenient and flexible is lacking, and the requirement that the culture can be sampled for many times without destroying the anaerobic environment while the rotating speed of the culture can be controlled and the oxygen content in a system can be monitored in the process of one-time anaerobic culture can be met. There is therefore a need for a method or apparatus that addresses the above-mentioned problems.

Disclosure of Invention

The utility model provides an anaerobic fermentation device which solves the defects of the prior art, has simple structure, ingenious design, simple and convenient operation and low cost, and can carry out sampling operation for a plurality of times.

The technical solution of the utility model is that: a low-cost anaerobic fermentation device capable of sampling for multiple times is characterized in that: the device comprises a nitrogen bottle 1, the nitrogen bottle 1 is connected with a first pipeline 3 through a pressure reducing valve 2, a degerming air filter 4 and a valve 5 are arranged on the first pipeline 3, the outlet end of the first pipeline 3 is positioned in a sample bottle 6, the outlet of the first pipeline 3 is positioned at the bottom of the sample bottle 6, a second pipeline 7 is connected onto the sample bottle 6, the valve 5 is arranged on the second pipeline 7, meanwhile, the end part of the second pipeline 7 is positioned at the bottom of the sample bottle 6, the sample bottle 6 is also communicated with a state detection bottle 9 through a third pipeline 8, two ends of the third pipeline 8 are respectively positioned at the upper parts of the sample bottle 6 and the state detection bottle 9, the degerming air filter 4 and the valve 5 are arranged on the third pipeline 8, a fourth pipeline 10 and a fifth pipeline 11 are arranged on the state detection bottle 9, a pressure vacuum meter 12 is arranged at the tail end of the fourth pipeline 10, the fifth pipeline 11 is provided with a valve 5, the end part of the fifth pipeline 11 is positioned at the bottom of the state detection bottle 9, and an oxygen indicator 13 is also placed in the state detection bottle 9.

Compared with the prior art, the utility model, have following advantage:

the low-cost anaerobic fermentation device with the structural form and the capability of sampling for multiple times has the advantages of simple structure, ingenious design and reasonable layout, and can be used in combination with a conventional shaking table in a laboratory, accurately control the rotating speed of anaerobic culture and conveniently realize liquid oscillation culture of anaerobic bacteria; the pressure and the oxygen content in a system are accurately monitored by arranging a state detection bottle and a matched pipeline; and continuous sampling can be realized without destroying anaerobic environment in the culture process. Compared with the traditional equipment for realizing the same function, the equipment has lower input cost and is safer and more convenient to use. Therefore, the method has multiple advantages, is particularly suitable for popularization and application in the field, and has very wide market prospect.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention.

Detailed Description

The following description will explain embodiments of the present invention with reference to the drawings. As shown in fig. 1: a low-cost anaerobic fermentation device capable of sampling for many times comprises a nitrogen bottle 1, wherein the nitrogen bottle 1 is connected with a first pipeline 3 through a pressure reducing valve 2, a degerming air filter 4 and a valve 5 are arranged on the first pipeline 3, the outlet end of the first pipeline 3 is positioned in a sample bottle 6, the outlet of the first pipeline 3 is positioned at the bottom of the sample bottle 6, a second pipeline 7 is connected with the sample bottle 6, the second pipeline 7 is provided with the valve 5, meanwhile, the end part of the second pipeline 7 is positioned at the bottom of the sample bottle 6, the sample bottle 6 is also communicated with a state detection bottle 9 through a third pipeline 8, the two ends of the third pipeline 8 are respectively positioned at the upper parts inside the sample bottle 6 and the state detection bottle 9, the third pipeline 8 is provided with the degerming air filter 4 and the valve 5, the state detection bottle 9 is provided with a fourth pipeline 10 and a fifth pipeline 11, the tail end of the fourth pipeline 10 is provided with a pressure vacuum gauge 12, the fifth pipeline 11 is provided with a valve 5, the end part of the fifth pipeline 11 is positioned at the bottom of the state detection bottle 9, and an oxygen indicator 13 is also placed in the state detection bottle 9.

The working process of the low-cost anaerobic fermentation device with the structure form and capable of sampling for multiple times is as follows:

the method comprises the steps of firstly filling a liquid culture medium with a proper volume into a sample bottle 6, screwing a sealing cover without screwing, and leaving a gap, closing three valves 5 on a first pipeline 3, a second pipeline 7 and a third pipeline 8, and wrapping the outer tail end of the second pipeline 7 by kraft paper. Putting the sample bottle 6, the first pipeline, the second pipeline, the third pipeline and all the sterilizing air filters 4 and valves 5 on the 3 pipelines into a sterilizing pot, performing high-pressure steam sterilization according to process parameters, taking the sample bottle 6 out of the pot at about 95 ℃ after the sterilization is finished, and immediately screwing a sealing cover of the sample bottle 6 to prevent oxygen from entering a system. The first pipeline 3 is connected with the nitrogen pressure reducing valve 2, inoculation operation is carried out after the culture medium is cooled to room temperature, and a proper amount of nitrogen is introduced into the sample bottle 6 in the cooling process to prevent negative pressure. Under aseptic operation in the clean bench, seed solution can be added to the sample bottle 6 through the second line 7, after which the valve 5 on the second line 7 is closed quickly.

The following operations can be performed outside the clean bench:

the third pipe 8 is connected to the state detecting bottle 9, the oxygen indicator 13 is put into the state detecting bottle 9, and the sealing cap is tightened. Opening a pressure reducing valve 2 and valves 5 on a first pipeline 3, a third pipeline 8 and a fifth pipeline 11, introducing high-purity nitrogen into a sample bottle 6 through the first pipeline 3, exhausting air in the sample bottle 6 and a state detection bottle 9, judging whether the state detection bottle 9 is in an anaerobic environment or not by observing the color of an oxygen indicator 13 by an operator, closing the valve 5 on the fifth pipeline 11 if the air in the state detection bottle 9 is exhausted, continuously inputting nitrogen into the sample bottle 6 and the state detection bottle 9, gradually increasing the air pressure in the two bottles, observing the reading on a pressure gauge 12 by the operator, closing the pressure reducing valve 2 after the pressure in the two bottles reaches a preset value, and judging that the air pressure value and the oxygen content in the two bottles are equal because the two bottles are communicated through the third pipeline 8, wherein the parameters can be judged through the color display of the oxygen indicator 13 in the state detection bottle 9 and the pressure gauge 12 on the two bottles To do so.

Then closing the valve 5 on the first pipeline 3, detaching the first pipeline 3 from the pressure reducing valve 2, and simultaneously placing the sample bottle 6 and the state detection bottle 9 which are connected into an integral structure on a temperature-controlled shaking table for oscillation treatment, so as to realize the oscillation culture operation of the bacterial liquid in the anaerobic environment (according to the experimental purpose, the bacterial liquid can also be placed in an incubator for static culture); after the cultivation, at the end connection sampling bottle of second pipeline 7 to open valve 5 on the second pipeline 7, because the atmospheric pressure in the sample bottle 6 is higher relatively, consequently under the effect of atmospheric pressure, the fungus liquid can get into the sampling bottle through second pipeline 7 is automatic, through this kind of mode, can realize taking a sample many times.

Claims (1)

1. A low-cost anaerobic fermentation device capable of sampling for multiple times is characterized in that: the device comprises a nitrogen bottle (1), wherein the nitrogen bottle (1) is connected with a first pipeline (3) through a pressure reducing valve (2), a sterilizing air filter (4) and a valve (5) are arranged on the first pipeline (3), the outlet end of the first pipeline (3) is positioned in a sample bottle (6), and the outlet of the first pipeline is positioned at the bottom of the sample bottle (6); a second pipeline (7) is connected to the sample bottle (6), a valve (5) is arranged on the second pipeline (7), and the end part of the second pipeline (7) is positioned at the bottom of the sample bottle (6); the sample bottle (6) is also communicated with the state detection bottle (9) through a third pipeline (8), two ends of the third pipeline (8) are respectively positioned at the upper parts of the sample bottle (6) and the state detection bottle (9), and the third pipeline (8) is provided with a sterilizing air filter (4) and a valve (5); the state detection bottle is characterized in that a fourth pipeline (10) and a fifth pipeline (11) are arranged on the state detection bottle (9), a pressure vacuum meter (12) is arranged at the tail end of the fourth pipeline (10), a sterilizing air filter (4) and a valve (5) are further arranged on the fourth pipeline (10), the valve (5) is arranged on the fifth pipeline (11), the end portion of the fifth pipeline (11) is located at the bottom of the state detection bottle (9), and an oxygen indicator (13) is further placed in the state detection bottle (9).

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