CN207405183U - A kind of cell culture apparatus for monitoring cell culture liquid environment - Google Patents
A kind of cell culture apparatus for monitoring cell culture liquid environment Download PDFInfo
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- CN207405183U CN207405183U CN201720740866.5U CN201720740866U CN207405183U CN 207405183 U CN207405183 U CN 207405183U CN 201720740866 U CN201720740866 U CN 201720740866U CN 207405183 U CN207405183 U CN 207405183U
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Abstract
The utility model provides a kind of cell culture apparatus for monitoring cell culture liquid environment, including culture dish, culture dish is sealing structure, cell culture fluid and cell are equipped in it, the outside one end of connection equipped with hose of culture dish, the other end of hose fix connection sampling ware, and the ware for sampling ware covers fixation and is communicated with negative tube, negative tube is mounted on negative pressure air pump, and hose is equipped with flow control valve.When the utility model is sampled monitoring to culture solution in culture dish, air in sampling ware is extracted out by negative pressure air pump, reduce the pressure in sampling ware, under pressure difference effect inside and outside sampling ware, cell culture fluid in culture dish is transported to by hose in sampling ware, wherein ware lid is movably arranged on by helicitic texture on ware body, pass through the dismounting between ware body and ware lid, the convenient cell culture fluid in sampling ware is sampled detection, so as to avoid directly opening culture dish and caused by culture dish inner equilibrium environment disruption.
Description
Technical field
The utility model is related to field of cell culture, are specially a kind of cell culture for monitoring cell culture liquid environment
Device.
Background technology
Cell injuring model technology is that mammalian cell is inoculated in the liquid culture medium in cell culture container,
In containing multiple nutritional components necessary to cell growth breeding, and place it in specific temperature, humidity, gas can be set
Culture is incubated in the cell incubator of environment, is allowed to grow, be proliferated.In scientific research, it is often necessary to study cell culture fluid
In certain predetermined substance processes such as cell growth, division, multiplication under certain concentration influence.In previous process of experimental
The concentration for monitoring predetermined substance in cell culture liquid is needed to change, multiple culture solution sampling time points and detection are typically set
Number obtains dynamic data, when carrying out anoxic or hyperoxia cell culture, opens culture chamber door acquisition cell culture fluid process
It is middle to cause the variation of incubator gaseous environment, and the time is needed to balance again, the accuracy of experimental result is influenced, for this purpose,
It is proposed that a kind of cell culture apparatus for monitoring cell culture liquid environment.
Utility model content
The purpose of this utility model is to provide a kind of cell culture apparatus for monitoring cell culture liquid environment, with solution
Certainly the problems mentioned above in the background art.
To achieve the above object, the utility model provides following technical solution：One kind can monitor cell culture liquid environment
Cell culture apparatus, including culture dish, the culture dish be sealing structure, in be equipped with cell culture fluid and cell, it is described
The outside one end of connection equipped with hose of culture dish, the other end of the hose fix connection sampling ware, the ware of the sampling ware
It covers to fix and is communicated with negative tube, the negative tube is mounted on negative pressure air pump, and hose is equipped with flow control valve.
Preferably, the sampling ware includes ware body and ware lid, and the ware lid is movably arranged on by helicitic texture on ware body,
By the dismounting between ware body and ware lid, the convenient cell culture fluid in sampling ware is sampled detection.
Preferably, the top of the negative pressure air pump connection sampling ware, the air in sampling ware, drop are extracted out by negative pressure air pump
Pressure in low sampling ware.
Preferably, the flow control valve includes valve body, and suppression slot is equipped in the valve body, and the hose vertically runs through suppression
Slot, and the opening of suppression slot is equipped with apical axis, the apical axis inner end and the contact of hose outer surface by helicitic texture, and outer end is consolidated
Surely it is equipped with spin-block.
Preferably, the suppression slot is columnar structures, and suppression groove bottom on the right side of hose is dome-type, the apical axis
Inner end for dome-type structure, and outer end is cylindrical structure.
Compared with prior art, the beneficial effects of the utility model are：The utility model is easy to operate, and sampling precision is high,
And it is smaller on the environment influence in culture dish, it is specific as follows：
When the 1st, being sampled monitoring to culture solution in culture dish, the air in sampling ware is extracted out by negative pressure air pump, is reduced
The pressure in ware is sampled, under pressure difference effect inside and outside sampling ware, the cell culture fluid in culture dish is transported to by hose
It samples in ware；
2nd, ware lid is movably arranged on by helicitic texture on ware body, convenient to taking by the dismounting between ware body and ware lid
Cell culture fluid in sample ware is sampled detection, so as to avoid directly opening culture dish and caused by culture dish inner equilibrium environment
Imbalance；
3rd, by rotating apical axis, its inner end is made to be moved in suppression slot, and then squeezes or unclamp hose, reach control hose
The effect of interior flow, cell culture fluid flows into the amount in sampling ware during so as to control each sampling monitoring.
Description of the drawings
Fig. 1 is the utility model structure diagram；
Fig. 2 is flow control valve structure diagram in the utility model.
In figure：1 culture dish, 2 hoses, 3 flow control valves, 301 valve bodies, 302 suppression slots, 303 apical axis, 304 spin-blocks, 4 sampling wares,
5 negative tubes, 6 negative pressure air pumps.
Specific embodiment
The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out
It clearly and completely describes, it is clear that the described embodiments are only a part of the embodiments of the utility model rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are without making creative work
All other embodiments obtained shall fall within the protection scope of the present invention.
- 2 are please referred to Fig.1, the utility model provides a kind of technical solution：It is a kind of to monitor the thin of cell culture liquid environment
Born of the same parents' culture apparatus, including culture dish 1, culture dish 1 is sealing structure, in be equipped with cell culture fluid and cell, culture dish 1 it is outer
The side one end of connection equipped with hose 2, the other end of hose 2 fix connection sampling ware 4, and sampling ware 4 includes ware body and ware lid, ware lid
It is movably arranged on by helicitic texture on ware body, by the dismounting between ware body and ware lid, the convenient cell in sampling ware is trained
Nutrient solution is sampled detection, by being sampled the cell culture fluid in sampling ware 4, avoids directly opening culture dish 1 and making
Into 1 inner equilibrium environment disruption of culture dish.
The ware of sampling ware 4, which covers to fix, is communicated with negative tube 5, and negative tube 5 is mounted on negative pressure air pump 6, and negative pressure air pump 6 connects
The top of logical sampling ware 4 extracts the air in sampling ware 4 out by negative pressure air pump 6, reduces the pressure in sampling ware 4, in sampling ware
Inside and outside 4 under pressure difference effect, the cell culture fluid in culture dish 1 is transported to by hose in sampling ware 4, is conveniently sampled
Monitoring.
And hose 2 is equipped with flow control valve 3, flow control valve 3 includes valve body 301, suppression slot 302, hose 2 is equipped in valve body 301
Vertically through suppression slot 302, and the opening of suppression slot 302 is equipped with apical axis 303 by helicitic texture, 303 inner end of apical axis and soft
2 outer surface of pipe contacts, and outer end is fixed with spin-block 304, and suppression slot 302 is columnar structures, the suppression slot positioned at 2 right side of hose
302 bottom surfaces are dome-type, and the inner end of apical axis 303 is dome-type structure, and outer end is cylindrical structure, by rotating apical axis 303,
Make the movement in suppression slot 302 of its inner end, and then squeeze or unclamp hose 2, have the function that control flow in hose 2, so as to
Cell culture fluid flows into the amount in sampling ware 4 when controlling each sampling monitoring.
Operation principle：The utility model is taken out by negative pressure air pump 6 when culture solution is sampled monitoring in culture dish 1
Go out to sample the air in ware 4, reduce the pressure in sampling ware 4, it, will be in culture dish 1 under pressure difference effect inside and outside sampling ware 4
Cell culture fluid is transported to by hose in sampling ware 4, and wherein ware lid is movably arranged on by helicitic texture on ware body, passes through ware
Dismounting between body and ware lid, the convenient cell culture fluid in sampling ware are sampled detection, so as to avoid directly opening training
Support ware 1 and caused by 1 inner equilibrium environment disruption of culture dish, by rotating apical axis 303, make the movement in suppression slot 302 of its inner end,
And then squeeze or unclamp hose 2, have the function that control flow in hose 2, cell culture during so as to control each sampling monitoring
Liquid stream enters to sample the amount in ware 4.
While there has been shown and described that the embodiment of the utility model, for the ordinary skill in the art,
It is appreciated that in the case where not departing from the principle of the utility model and spirit can these embodiments be carried out with a variety of variations, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (5)
1. a kind of cell culture apparatus for monitoring cell culture liquid environment, including culture dish (1), the culture dish (1) is
Sealing structure, it is interior to be equipped with cell culture fluid and cell, it is characterised in that：The outside connection of the culture dish (1) is equipped with hose
(2) one end, the other end of the hose (2) fix connection sampling ware (4), and the ware of the sampling ware (4) covers fixed connection
There is negative tube (5), the negative tube (5) is mounted on negative pressure air pump (6), and hose (2) is equipped with flow control valve (3).
2. a kind of cell culture apparatus for monitoring cell culture liquid environment according to claim 1, it is characterised in that：
The sampling ware (4) includes ware body and ware lid, and the ware lid is movably arranged on by helicitic texture on ware body, passes through ware body and ware
Dismounting between lid, the convenient cell culture fluid in sampling ware are sampled detection.
3. a kind of cell culture apparatus for monitoring cell culture liquid environment according to claim 1, it is characterised in that：
The top of negative pressure air pump (6) the connection sampling ware (4) is extracted the air in sampling ware (4) out by negative pressure air pump (6), is reduced
Sample the pressure in ware (4).
4. a kind of cell culture apparatus for monitoring cell culture liquid environment according to claim 1, it is characterised in that：
The flow control valve (3) includes valve body (301), is equipped with suppression slot (302) in the valve body (301), the hose (2) is vertically run through
Suppression slot (302), and the opening of suppression slot (302) is equipped with apical axis (303), apical axis (303) inner end by helicitic texture
It is contacted with hose (2) outer surface, and outer end is fixed with spin-block (304).
5. a kind of cell culture apparatus for monitoring cell culture liquid environment according to claim 4, it is characterised in that：
The suppression slot (302) is columnar structures, and suppression slot (302) bottom surface on the right side of hose (2) is dome-type, the apical axis
(303) inner end is dome-type structure, and outer end is cylindrical structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720740866.5U CN207405183U (en) | 2017-06-23 | 2017-06-23 | A kind of cell culture apparatus for monitoring cell culture liquid environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720740866.5U CN207405183U (en) | 2017-06-23 | 2017-06-23 | A kind of cell culture apparatus for monitoring cell culture liquid environment |
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| Publication Number | Publication Date |
|---|---|
| CN207405183U true CN207405183U (en) | 2018-05-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201720740866.5U Active CN207405183U (en) | 2017-06-23 | 2017-06-23 | A kind of cell culture apparatus for monitoring cell culture liquid environment |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110184300A (en) * | 2019-05-21 | 2019-08-30 | 安徽环球基因科技有限公司 | A method of memebrane protein is produced using stable cell line |
| CN114149921A (en) * | 2021-12-09 | 2022-03-08 | 中国人民解放军空军军医大学 | Tumor complex microenvironment cell culture device |
-
2017
- 2017-06-23 CN CN201720740866.5U patent/CN207405183U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110184300A (en) * | 2019-05-21 | 2019-08-30 | 安徽环球基因科技有限公司 | A method of memebrane protein is produced using stable cell line |
| CN114149921A (en) * | 2021-12-09 | 2022-03-08 | 中国人民解放军空军军医大学 | Tumor complex microenvironment cell culture device |
| CN114149921B (en) * | 2021-12-09 | 2023-07-25 | 中国人民解放军空军军医大学 | Tumor complex microenvironment cell culture device |
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