CN205473761U - Cell liquid shifts bottle - Google Patents

Abstract

The utility model relates to a biological experiment device technical field especially involves a cell liquid shifts bottle. Including the reagent bottle, have a middle bottleneck, two first bottlenecks that lie in these centre bottleneck both sides, stainless steel bi -pass board is provided with the silica gel gasket, stainless steel bi -pass board is used for sealing the middle bottleneck of reagent bottle, first silicone tube and second silicone tube are connected respectively stainless steel bi -pass board is in order to pass through stainless steel bi -pass board intercommunication the reagent bottle, is the second silicone tube through a bi -pass articulate C the flex pipe, C the flex pipe still is connected with the gaseous sterilization filter of second. The utility model discloses in the use, cell liquid shifts bottle's middle bottle lid is not opened, and selects one side bottle lid to open and carry out aseptic technique, and inside gasket is fixed on the bottle lid and is not had inside silicone tube, so greatly reduced the risk of bacterial contamination, the success rate of at utmost assurance cell culture technology.

Description

A kind of Cell sap transfer bottle

Technical field

This utility model relates to biotic experiment equipment technical field, refers more particularly to a kind of Cell sap Transfer bottle.

Background technology

The technological requirement of cell culture technology is relatively stricter, its main and other common laboratory works That makees differs primarily in that requirement keeps sterile working completely, it is to avoid microorganism and extraneous other The impact of harmful factor.The pollution behave that prevents of cell culture technology is to determine that cell cultivates success or failure Key, even if well-appointed laboratory, if experimenter is careless, operating technology is not Specification, also results in pollution, therefore all operations will the guarantee of maximum possible aseptic.Such as: Cell needs to be transferred to by the cell culture fluid in shaking flask in seed transfer bottle in incubation, Existing single port seed transfer bottle internal washer when opening top bottle cap easily drops or bottle Internal silica gel tube easily touches when taking out and carries disease germs object and germ contamination occurs, and this all can drop significantly The success rate of low cell culture process.

Utility model content

In view of above-mentioned technical problem defect, this utility model provides a kind of Cell sap transfer bottle, This Cell sap transfer bottle is primarily to avoid sampling operation in cell culture process development process Easily cause the germ contamination of cell culture fluid in bioreactor, at utmost ensure that cell is cultivated The success rate of technique.

The technical scheme that this utility model is used for above-mentioned technological deficiency is:

A kind of Cell sap transfer bottle, wherein, described Cell sap transfer bottle includes:

Reagent bottle, has middle bottleneck, two be positioned at these middle bottleneck both sides first bottles Mouthful；

Rustless steel two-way plate, is provided with silica gel pad, and described rustless steel two-way plate is used for sealing institute State the middle bottleneck of reagent bottle；

First silica gel tube and the second silica gel tube, connect described rustless steel two-way plate, respectively to pass through Described rustless steel two-way plate connects described reagent bottle；

Described second silica gel tube connects C-Flex by two pass joints and manages, described C-Flex Pipe is also associated with the second gas sterilizing filter.

As preferably, above-mentioned Cell sap shifts bottle, wherein, described middle bottleneck, two institutes State the first bottleneck all to be sealed by bottle cap；

Wherein, described rustless steel two-way plate is arranged in the bottle cap that described middle bottleneck is corresponding.

As preferably, above-mentioned Cell sap transfer bottle, wherein, described first silica gel tube is also connected with There is the first sterilizing filter.

Technique scheme has the advantage that or beneficial effect:

The utility model discloses a kind of Cell sap transfer bottle, in use, Cell sap turns The middle bottle cap moving bottle is not switched on, and selects side bottle cap to open and carry out sterile working, inner mast Sheet is fixed on bottle cap and does not has internal silica gel tube, and then greatly reduces the wind of germ contamination Danger, at utmost ensures the success rate of cell culture process.

Accompanying drawing explanation

The detailed description with reference to the following drawings, non-limiting example made by reading, this reality Will become more apparent by novel and feature, profile and advantage.In whole accompanying drawings identical The labelling identical part of instruction.Can not be drawn to scale accompanying drawing, it is preferred that emphasis is illustrate Purport of the present utility model.

Fig. 1 is the structural representation of Cell sap transfer bottle in this utility model embodiment.

Detailed description of the invention

With specific embodiment, this utility model is further described below in conjunction with the accompanying drawings, but It is not as restriction of the present utility model.

In view of, in conventional solution, cell needs to cultivate the cell in shaking flask in incubation Liquid is transferred in seed transfer bottle, and existing single port seed transfer bottle is interior when opening top bottle cap Easily touch when portion's packing ring easily drops or bottle interior silica gel tube takes out and carry disease germs object and occur Germ contamination, this all can be substantially reduced the success rate of cell culture process.Therefore, this practicality is new Type provides a kind of Cell sap transfer bottle, and this Cell sap transfer bottle is primarily to avoid cell to train During supporting process exploitation, sampling operation easily causes the antibacterial of cell culture fluid in bioreactor Pollute, at utmost ensure the success rate of cell culture process.

Concrete, the structure of Cell sap transfer bottle in this utility model embodiment as shown in Figure 1 Schematic diagram, this Cell sap transfer bottle includes:

Reagent bottle 1, have bottleneck in the middle of, two be positioned at the first of these middle bottleneck both sides Bottleneck；

Rustless steel two-way plate 4, is provided with silica gel pad 3, and described rustless steel two-way plate 4 is used for Seal the middle bottleneck of described reagent bottle 1；

First silica gel tube 5 and the second silica gel tube 7, connects described rustless steel two-way plate 4 respectively, To connect described reagent bottle 1 by described rustless steel two-way plate 4；

Described second silica gel tube 7 connects C-Flex by two pass joints 8 and manages (Carbon Fiber Flex manages) 9, described C-Flex pipe 9 is also associated with the second gas sterilizing filter 10.

In embodiment of the present utility model, described middle bottleneck, two described first bottlenecks are equal Sealed by bottle cap 2；

Wherein, described rustless steel two-way plate 4 is arranged in the bottle cap that described middle bottleneck is corresponding.

As preferably, described first silica gel tube 5 is also associated with the first sterilizing filter 6, its In, the second silica gel tube 7 is extended in described reagent bottle 1 by described rustless steel two-way plate 4 Bottom.

This utility model Cell sap transfer bottle is during using, by two gas aseptic filtrations Device, reagent bottle, rustless steel two-way plate etc. are managed by some silica gel tubes and C-Flex and are pressed Fig. 1 institute Show and be attached, after connecting, be positioned over sterilizing in steriliser.Occupation mode is as follows: ultra-clean Open any one in the bottle cap of both sides under platform, use liquid-transfering gun by Cell sap by the bottle opened Lid is injected in reagent bottle, tightens bottle cap, by C-Flex pipe 9 and reactor to be seeded C-Flex pipe carries out aseptic connection by aseptic pipeline bonding machine, then by the cell in reagent bottle In liquid injecting reactor, complete the inoculation operation of reactor.During whole use, cell The middle bottle cap of liquid transfer bottle is not switched on, and selects side bottle cap to open and carry out sterile working, interior Portion's pad is fixed on bottle cap and does not has internal silica gel tube, and then greatly reduces germ contamination Risk, at utmost ensure the success rate of cell culture process.

It should be appreciated by those skilled in the art that those skilled in the art combine prior art and Above-described embodiment can realize described change case, does not repeats at this.Such change case is not Affect flesh and blood of the present utility model, do not repeat them here.

Above preferred embodiment of the present utility model is described.It is to be appreciated that this Utility model is not limited to above-mentioned particular implementation, the equipment that do not describes in detail the most to the greatest extent and Structure is construed as being practiced with the common mode in this area；Any it is familiar with this area Technical staff, without departing under technical solutions of the utility model ambit, may utilize above-mentioned Technical solutions of the utility model are made many possible variations by the method disclosed and technology contents And modification, or it being revised as the Equivalent embodiments of equivalent variations, this has no effect on of the present utility model Flesh and blood.Therefore, every content without departing from technical solutions of the utility model, according to this reality And repair any simple modification made for any of the above embodiments, equivalent variations by novel technical spirit Decorations, all still fall within the range of technical solutions of the utility model protection.

Claims (3)

1. a Cell sap transfer bottle, it is characterised in that described Cell sap transfer bottle includes:

Reagent bottle, has middle bottleneck, two first bottlenecks being positioned at these middle bottleneck both sides；

Rustless steel two-way plate, is provided with silica gel pad, and described rustless steel two-way plate is for sealing the middle bottleneck of described reagent bottle；

First silica gel tube and the second silica gel tube, connect described rustless steel two-way plate respectively, to connect described reagent bottle by described rustless steel two-way plate；

Described second silica gel tube connects C-Flex by two pass joints and manages, and described C-Flex pipe is also associated with the second gas sterilizing filter.

Cell sap the most according to claim 1 transfer bottle, it is characterised in that described middle bottleneck, two described first bottlenecks are all sealed by bottle cap；

Wherein, described rustless steel two-way plate is arranged in the bottle cap that described middle bottleneck is corresponding.

Cell sap the most according to claim 1 transfer bottle, it is characterised in that described first silica gel tube is also associated with the first sterilizing filter.