CN209624623U - Liquid channel system for chemical illumination immunity analysis instrument - Google Patents
Liquid channel system for chemical illumination immunity analysis instrument Download PDFInfo
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- CN209624623U CN209624623U CN201920240032.7U CN201920240032U CN209624623U CN 209624623 U CN209624623 U CN 209624623U CN 201920240032 U CN201920240032 U CN 201920240032U CN 209624623 U CN209624623 U CN 209624623U
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Abstract
The utility model discloses a kind of liquid channel systems for chemical illumination immunity analysis instrument, including sample sampling unit, reagent sampling unit, substrate unit, magnetic bead cleaning unit and waste disposal unit, magnetic bead cleaning unit includes cleaning module and drawing liquid module, cleaning module includes topping-up pump and the first washing lotion bucket, topping-up pump outlet end is connected with multichannel fluid injection pipeline, is in series with injection needle and the first solenoid valve in the dispensing liquid pipe of multichannel fluid injection pipeline；Second solenoid valve, flushing pump and two-position three-way valve are in series in flushing pipe between topping-up pump and the first washing lotion bucket, two-position three-way valve third end is connected with the second washing lotion bucket.The utility model structure is simple, quantitative accurate, and reagent or sample is avoided cross contamination occur, and magnetic bead cleaning unit guarantees that reservoir quantity is consistent consistent with drawing liquid amount, improves detection success rate；System stability is good, and detection accuracy is high, favorable repeatability, reduces by systematic error or sample, reagent cross contamination and the probability for the detection failure that causes to shine.
Description
Technical field
The utility model relates to the sample processing systems of chemical illumination immunity analysis instrument, more particularly, to one kind for chemistry
The liquid channel system of luminescence immunoassay instrument.
Background technique
Full-automatic chemiluminescence immunoassay analysis meter has the characteristics that environmentally friendly, quick, accurate to be widely used in scientific research, vitro detection
Etc. technical fields.Chemical illumination immunity analysis instrument liquid channel system is to guarantee accurate systematic quantification, stable operation and realize repeatability
The core execution module of detection, including sample/reagent modules, substrate module, magnetic bead cleaning module and liquid waste processing module.
Currently, liquid channel system have sample quantify failure rate it is high, there are cross contamination, repeatability it is poor etc. technologies ask
Topic, specific as follows: first, sample/reagent sampling module sampling probe aperture is smaller, is easy for a long time using rear sample or reagent
It is attached on sampling probe inner wall, and the pressure in the speed combination detergent line that traditional sample/reagent sampling module passes through plunger pump
Whether power judges sampling probe blocking jointly, but since the attraction speed of plunger pump is exaggerated the error of sampling quantity, it can not confirm
Whether sampling probe is rinsed well, so that there is the situation and the case where there are sample/reagent cross contaminations of quantitatively failing, sample in the module
This detection failure rate that shines is high.Secondly, the pipeline of existing magnetic bead cleaning module is complicated and does not have self-cleaning function, so that instrument
Crystallinity cleaning solution is liable to stick on injection needle inner wall and fluid injection pipeline inner wall after long-play, causes the stream of each subtube
Hinder not of uniform size, it is inconsistent to further result in cleaning solution reservoir quantity, and repeatability is poor；Peristaltic pump is easy to appear super negative when drawing liquid
Lotus operating condition, or even there is waste liquid suck-back phenomenon, the stable operation of system is seriously affected, instrument failure rate is high.Third, waste liquid
The outer cleaning effect of module is poor, and just with waste drains pump by sampling to waste liquid barrel, the outer wall hanging liquid of sampling probe is serious, causes
Sample cross contamination influences the reliability of testing result.4th, sampling probe is stainless pin, and extension liquid is serious, be easy to cause intersection
Pollution, repeatability are poor.5th, entire liquid channel system lacks prior-warning device, needs manually repeatedly to check waste liquid barrel or washing lotion bucket
Interior liquid level, high failure rate.
Summary of the invention
The utility model aim is to provide a kind of liquid channel system for chemical illumination immunity analysis instrument, improves sample and determine
Success rate is measured, cross contamination is avoided.
To achieve the above object, the utility model takes following technical proposals:
Liquid channel system described in the utility model for chemical illumination immunity analysis instrument, including sample sampling unit, examination
Agent sampling unit, substrate unit, magnetic bead cleaning unit and waste disposal unit, the magnetic bead cleaning unit include cleaning module and
Drawing liquid module, the cleaning module include topping-up pump and the first washing lotion bucket, and the outlet end of the topping-up pump is communicated with multichannel note
Liquid pipeline is in series with injection needle in each dispensing liquid pipe of the multichannel fluid injection pipeline and controls the injection needle reservoir quantity
The first solenoid valve；Spaced series have second solenoid valve and flushing in flushing pipe between the topping-up pump and the first washing lotion bucket
It pumps, is in series with two-position three-way valve, the two-bit triplet in the flushing pipe between the flushing pump and second solenoid valve
The third end of valve is connected by connecting tube with the second washing lotion bucket；The drawing liquid module includes the multichannel being connected with waste liquid barrel
Drawing liquid pipeline, each of described multichannel drawing liquid pipeline, which divides, is in series with aspirating needle and suction pumps on liquid suction pipe.
The substrate unit includes the identical substrate conveyor module of two structures, and the substrate conveyor module includes passing through substrate
The substrate bottle and substrate needle that pipeline is connected are in series with substrate pump on the substrate pipeline, the institute positioned at substrate pump two sides
It states and is in series with third solenoid valve on substrate pipeline.
The sample sampling unit is identical with the equal structure of reagent sampling unit, and sample sampling unit and reagent sampling are single
Member includes the sampling probe and sampling pump being connected by collection tube, is in series with second pressure sensor on the collection tube；Institute
It states sampling pump and is connected by detergent line with the first washing lotion bucket, the 4th solenoid valve and clear is in series in the detergent line
Pump is washed, is in series with the shunting being connected with the first washing lotion bucket in the detergent line between the cleaning pump and the 4th solenoid valve
It manages, is in series with first pressure sensor and overflow valve on the isocon.
Second pressure sensor is in series on the collection tube of the sample sampling unit.
The waste disposal unit includes for cleaning the service sink I of sample sampling unit, for cleaning reagent sampling list
Member service sink II, liquid trap and the waste liquid barrel, the condensate outlet of reagent altar, the magnetic bead cleaning unit waste liquid port,
First leakage fluid dram I of service sink I and the first leakage fluid dram II of service sink II pass through the import of drain pipe I and the liquid trap respectively
It is connected, the 5th solenoid valve is in series on each drain pipe I, the outlet of liquid trap is connected by sewer pipe with waste liquid barrel
It connects, positive displacement pump I is in series on the sewer pipe.
Second leakage fluid dram I of the service sink I and the second leakage fluid dram II of service sink II are in parallel by drain pipe II respectively
On the sewer pipe, positive displacement pump II of connecting on each drain pipe II is in series with the 6th on the drain pipe II of service sink I
Solenoid valve.
Two inlets of the service sink I are connected with the first washing lotion bucket, third washing lotion bucket respectively by inlet tube I
It connects, is in series with delivery pump I on each inlet tube I；The inlet of the service sink II is washed by inlet tube II with first
Liquid bucket is connected, and is in series with delivery pump II on the inlet tube II.
The topping-up pump, substrate pump and sampling pump are plunger pump；The flushing pump, cleaning pump, positive displacement pump I, positive displacement pump
II, delivery pump I and delivery pump II are diaphragm pump；The suction pumps are peristaltic pump.
Liquid level sensor is provided in the waste liquid barrel, the first washing lotion bucket, the second washing lotion bucket and third washing lotion bucket.
The outer surface of the sampling probe is provided with coated with Teflon protective layer.
The utility model advantage is that structure is simple, and cleaning effect is good inside and outside sampling probe, can be avoided reagent or sample goes out
Existing cross contamination, magnetic bead cleaning unit can guarantee that magnetic bead cleaning fluid injection is consistent with drawing liquid, and cleaning effect is good, the waste liquid row of system
Liquid speed degree is fast, improves the operation stability of chemical illumination immunity analysis instrument and the accuracy of pattern detection result, repeatability
It is good, it reduces by systematic error and sample, reagent cross-infection and the probability for the detection failure that causes to shine, is embodied in following
Several points:
(1), the magnetic bead cleaning unit of the utility model adjusts each dispensing liquid by the opening and closing time of the first solenoid valve of control
The differences in flow resistance of pipe accurately controls the reservoir quantity of each dispensing liquid pipe, guarantees that the reservoir quantity of each injection needle is consistent, and then guarantees clear
Wash effect；The crystallinity cleaning solution in the second washing lotion bucket is extracted by topping-up pump when cleaning, then passes through flushing pump, the first electromagnetism
A large amount of purified water is pumped to multichannel fluid injection pipeline and injection needle by valve, second solenoid valve, will be attached to pipeline inner wall and note
The crystallinity cleaning solution of liquid needle inner wall rinses, and crystallinity cleaning solution is entirely prevented to be attached to injection needle inner wall or multichannel note
On liquid pipeline, it is further ensured that the pipe resistance of each dispensing liquid pipe is consistent, and then guarantee that the reservoir quantity of each injection needle is identical, improves system
The stability of system and the accuracy of testing result；Multiple peristaltic pumps are to be avoided the occurrence of by a motor-driven synchronous peristaltic pump
The phenomenon for causing drawing liquid amount inconsistent because of pump speed difference, further decreases systematic error.
(2), the sample sampling unit of the utility model is identical with reagent sampling unit structure, can not only improve sample or
The sample-adding precision of reagent guarantees that sample or reagent quantitative are accurate, guarantees the cleaning effect of sampling probe inner wall；The second pressure can also be passed through
Force snesor and first pressure sensor monitored pipeline pressure, avoid pipe breakage or leakage because of caused by loine pressure exception
Phenomenon, moreover it is possible to whether precisely determining that blocking occurs for sampling probe by the pressure value of second pressure sensor, and then avoid not same
There is cross contamination in this or reagent.
(3), the utility model waste unit can into service sink continuous liquid supply, also make full use of principle of negative pressure drain, have
Effect avoids the outer wall hanging liquid of sampling probe, and cleaning effect is good, avoids cross contamination, and cleaning effect is good.Specifically, with I drain of service sink
For, positive displacement pump II is in the state that continues working, and the 6th solenoid valves, positive displacement pump II evacuates the waste liquid in service sink, when clear
When in wash pool I without the waste liquid continuously flowed, the 6th solenoid valve power-off, the abstraction of positive displacement pump II is located at the 6th solenoid valve, positive displacement pump II
Between air in drain pipe II form parital vacuum, the 6th solenoid valves, the high-speed flow that negative pressure is formed rapidly will sampling
Waste liquid on needle outer wall is pumped in waste liquid barrel, and sampling probe cleaning effect is good, avoids cross contamination.
(4), it is coated with Teflon protective layer on the sampling probe outer wall of the utility model, it is magnetized several to reduce sampling probe generation
Rate, further avoid occur because of caused by sampling probe surface magnetization quantitatively fail or pollution problem；Liquid level sensor can monitor
Liquid level in washing lotion bucket or waste liquid barrel avoids liquid level abnormal and causes unpredictalbe risk.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
Fig. 2 is the structural schematic diagram of sample sampling unit and reagent sampling unit in Fig. 1
Fig. 3 is the structural schematic diagram of magnetic bead cleaning unit in Fig. 1.
Fig. 4 is the structural schematic diagram of substrate unit in Fig. 1.
Fig. 5 is the structural schematic diagram of waste unit in Fig. 1.
Specific embodiment
As shown in Figure 1, 2, the liquid channel system described in the utility model for chemical illumination immunity analysis instrument, including sample
Sampling unit, reagent sampling unit, substrate unit, magnetic bead cleaning unit and waste disposal unit, the sample sampling unit and
The equal structure of reagent sampling unit is identical, by taking reagent sampling unit as an example: reagent sampling unit includes passing through 1.1 phase of collection tube
The sampling probe 1.2 and sampling pump 1.3(sampling pump 1.3 of connection are plunger pump), the sampling pump 1.3 passes through cleaning pipe 1.4 and the
Be loaded with purified water in one washing lotion bucket the first washing lotion of 2(bucket 2) outlet pipe 2.1 be connected, is in series in the cleaning pipe 1.4
Four solenoid valves 1.5 and cleaning pump 1.6(cleaning pump 1.6 are diaphragm pump), be located at the cleaning pump 1.6 and the 4th solenoid valve 1.5 it
Between cleaning pipe 1.4 on be in series with the isocon 1.7 being connected with the first washing lotion bucket 2, be in series with first on the isocon 1.7
Pressure sensor 1.8 and overflow valve 1.9；The outer surface of the sampling probe 1.2 is provided with coated with Teflon protective layer, and reduction takes
Magnetized probability occurs for sample needle 1.2, further avoids occurring quantitatively failing or polluting because of caused by 1.2 surface magnetization of sampling probe
Problem.When actual arrangement, the isocon 1.7 of reagent sampling unit and the isocon of sample sampling unit are connected in parallel on the first washing lotion bucket
On 2 return pipe 2.2, simplify pipeline.
As shown in Fig. 2, the sampling probe of sample sampling unit is easy blocking since sample viscosity is larger, sample is determined to be accurate
This sampling unit sampling probe stopping state is in series with second pressure sensor 1.10 on the collection tube of sample sampling unit.
The sampling of sample sampling unit and reagent sampling unit is identical with method for self-cleaning, by taking reagent sampling unit as an example:
Sampling probe 1.2 is moved in reagent bottle, the 4th solenoid valve 1.5 is adjusted and is in off-position, sampling pump 1.3 extracts reagent and moves back
It moves to predeterminated position, sampling pump 1.3 is by the specified reaction cup of the reagent injection of extraction, and then sampling probe 1.2 is moved to cleaning
II 7.2 top of pond, the 4th solenoid valve 1.5 of adjusting are energized state, start cleaning pump 1.6, with the purified water in the first washing lotion bucket 2
Continual rinsing sampling pump 1.3, sampling probe 1.2 and collection tube 1.1, the reagent that reagent sampling unit is adhered to are rinsed completely to cleaning
In pond II 7.2, the sampling and interior cleaning of reagent sampling unit are completed.Since sample viscosity is larger, sample sampling unit is carried out certainly
When cleaning, with the pressure value of the detection of second pressure sensor 1.10P A Subject to, whenP A Stop when in pre-set pressure range clear
It washes, the sampling probe of sample sampling unit is avoided to block, guarantee quantitative accuracy.
It is as follows that sample sampling unit sampling probe blocks decision process: 1.10 monitoring collection pipe 1.1 of second pressure sensor
PressureP A , single-chip microcontroller presets sample collection amount and isA n When corresponding pressureP A Range；When collection capacity isA n When, if pressureP A
On the contrary size is in default range, then illustrates that the sampling probe of sample sampling unit does not block, then illustrate sampling probe blocking；
When sampling probe 1.2 blocks, the 4th solenoid valves are adjusted, start cleaning pump, using purified water to sampling probe 1.2, collection tube
1.1 and sampling pump 1.3 cleaned, until pressureP A Stop cleaning when in preset range, avoid blocking, it is ensured that quantitative essence
Really.
Sample sampling unit is identical with the cleaning flow control methods of reagent sampling unit, by taking reagent sampling unit as an example:
Piping flow is positively correlated with loine pressure, and the relational expression of the two is as follows:, in formula, Q- flow,μ—
Discharge coefficient,A- pipe section product,P- loine pressure,ρThe density of-fluid is calculated by the pressure size of cleaning pipe 1.4
Whether the cleaning flow of cleaning pipe 1.4 meets 1.2 cleaning demand of sampling probe, and specifically, first pressure sensor 1.8 is for detecting
The pressure of 1.6 exit isocon 1.7 of cleaning pumpP B , pressureP B Then illustrate that cleaning flow can satisfy in pre-set interval to take
The cleaning demand of sample needle 1.2, works as pressureP B Greater than preset range maximum value when, then illustrate that the flow of isocon 1.7 is excessive, clear
The flow for washing pipe 1.4 is less than normal, and overflow valve 1.9 adjusts the flow of isocon 1.7 by throttle style, until pressureP B It is reduced to pre-
If in range, making 1.7 flow of isocon become smaller, the flow relative increase of cleaning pipe 1.4；Work as pressureP B Most less than preset range
When small value, then illustrate that the flow of isocon 1.7 is too small, the flow of cleaning pipe 1.4 is bigger than normal, overflow valve 1.9 is by way of overflow
Make pressureP B It increases in preset range, increases by 1.7 flow of isocon, the opposite reduction of 1.4 flow of cleaning pipe.
As shown in Figure 1,3, the magnetic bead cleaning unit includes cleaning module and drawing liquid module, and the cleaning module includes note
Liquid pump 3.1(topping-up pump 3.1 be plunger pump) and the first washing lotion bucket 2, the outlet end of the topping-up pump 3.1 be communicated with six channel fluid injections
Pipeline is in series with the one of injection needle 3.3 of injection needle 3.3(in each dispensing liquid pipe 3.2 of the six channels fluid injection pipeline
For the sleeve needle with drawing liquid and liquid injecting function) and control 3.3 reservoir quantity of injection needle the first solenoid valve 3.4；The note
Spaced series have second solenoid valve 3.6 and flushing pump 3.7(to rinse in flushing pipe 3.5 between liquid pump 3.1 and the first washing lotion bucket 2
Pump 3.7 is diaphragm pump), flushing pipe 3.5 is connected with the outlet pipe 2.1 of the first washing lotion bucket 2, positioned at the flushing pump 3.7 and the
It is specially two-bit triplet solenoid valve that two-position three-way valve 3.8(is in series in the flushing pipe 3.5 between two solenoid valves 3.6), institute
The third end for stating two-position three-way valve 3.8 is connected by connecting tube 3.9 with the second washing lotion bucket 4；The drawing liquid module includes and gives up
The six channel drawing liquid pipelines that 5 inlet tube 5.1 of liquid bucket is connected, each of described six channels drawing liquid pipeline divide on liquid suction pipe 3.10
Be in series with aspirating needle 3.11 and suction pumps 3.12, one of aspirating needle 3.11 is sleeve needle, the import of the sleeve needle with one
Dispensing liquid pipe 3.2 is connected；Suction pumps 3.12 are peristaltic pump, and six suction pumps 3.12 are by a motor driven, i.e., six suctions
Liquid pump 3.12 is synchronous working, guarantees that the pump speed of six suction pumps 3.12 is equal, and the cleaning that can be extracted simultaneously in 6 reaction cups is useless
Liquid, while can guarantee that drawing liquid amount is consistent, synchronism is good, avoids causing drawing liquid amount inconsistent because of pump speed difference.
It is provided with five groups of injection needles 3.3 as shown in figure 3, intersecting when actual arrangement, on the needle stand of magnetic bead cleaning unit and inhales
Liquid needle 3.11, positioned at right side needle be the sleeve needle with two kinds of functions of drawing liquid and fluid injection, the import of sleeve needle and one of them
Dispensing liquid pipe 3.2 is connected, and the outlet of sleeve needle divides liquid suction pipe 3.12 to be connected with one of them, and the needle positioned at left side is substrate
Needle 6.3.
The magnetic bead in reaction cup first is cleaned with the magnetic bead cleaning solution (crystallinity washing lotion) in the second washing lotion bucket 4 when cleaning,
Then it is cleaned again with the purified water in the first washing lotion bucket 2, avoids six channel fluid injection pipelines and injection needle 3.3 from blocking, detailed process
As follows: two-position three-way valve 3.8 connects the second washing lotion bucket 4, and the power-off of the first solenoid valve 3.4, second solenoid valve 3.6 are powered, topping-up pump
3.1 extract the cleaning solution in the second washing lotion bucket 4, and then the first solenoid valve 3.4 is powered, second solenoid valve 3.6 powers off, topping-up pump
3.1 inject the cleaning solution of extraction in reaction cup through each dispensing liquid pipe 3.2 and injection needle 3.3, a complete cleaning action；Then it inhales
Liquid pump 3.12 works asynchronously, and the cleaning waste liquid in reaction cup is extracted to waste liquid barrel 5；Carry out purified water cleaning again, two three
Port valve 3.8 is connected with the first washing lotion bucket 2, and the first solenoid valve 3.4 is powered, second solenoid valve 3.6 is powered, and flushing pump 3.7 works
Purifying water flow is increased, topping-up pump 3.1 passes through drawing liquid, spits liquid in purified water injection reaction cup, completes injection needle 3.3, fluid injection
The cleaning of pump 3.1 and multichannel fluid injection pipeline, avoids crystallinity washing lotion from being adhered to 3.3 inner wall of injection needle or multichannel fluid injection pipeline
Inner wall is further ensured that the flow resistance of each dispensing liquid pipe 3.2 is consistent, and then guarantees that the reservoir quantity of each injection needle 3.3 is identical, improves
The stability of system and the accuracy of testing result；Waste liquid barrel 5 is evacuated to by waste liquid is cleaned by suction pumps 3.12 after the completion of cleaning
It is interior, complete the cleaning of magnetic bead.In cleaning process, it can be rushed individually for a certain dispensing liquid pipe 3.2 and injection needle 3.3
It washes, can also be rinsed for any two of them or three or four or five dispensing liquid pipes 3.2, naturally it is also possible to simultaneously
Rinse six dispensing liquid pipes 3.2.
As shown in Fig. 1,4, the substrate unit includes the identical substrate conveyor module of two structures, two substrate conveyor modules
It is respectively used to addition A liquid (A liquid main component is luminol) and B liquid (B liquid main component is peroxidase), it is not same to meet
This luminous detection demand；The substrate conveyor module includes the substrate bottle 6.2 and substrate needle being connected by substrate pipeline 6.1
6.3, it is plunger pump that substrate pump 6.4(substrate pump 6.4 is in series on the substrate pipeline 6.1), it is located at substrate pump 6.4 and institute
It states and is in series with third solenoid valve I 6.5 on the substrate pipeline 6.1 between substrate needle 6.3, be located at substrate pump 6.4 and the substrate bottle
Third solenoid valve II 6.6 is in series on substrate pipeline 6.1 between 6.2.When work, shone detection demand according to sample, Xiang Sheng
A liquid or B liquid are added in reaction cup equipped with sample, the power-off of third solenoid valve I 6.5, third solenoid valve II 6.6 are powered, substrate pump
6.4 extract the A liquid (or B liquid) in substrate bottle 6.2, and then third solenoid valve I 6.5 is powered, third solenoid valve II 6.6 powers off, bottom
Object pump 6.4 spits liquid outward, and A liquid (or B liquid) is injected in reaction cup by substrate pipeline 6.1 through substrate needle 6.3, completes adding for substrate
Sample.
As shown in Figure 1,5, the waste disposal unit includes for cleaning the service sink I 7.1 of sample sampling unit, being used for
Service sink II 7.2, liquid trap 7.3 and the waste liquid barrel 5 of cleaning reagent sampling unit, two inlets of the service sink I 7.1
Alkaline rinse is loaded with the first washing lotion bucket 2, third washing lotion bucket 8(respectively by inlet tube I 7.4) it is connected, inlet tube I
It is diaphragm pump that I 7.5(delivery pump I 7.5 of delivery pump is in series on 7.4), two inlet tubes I 7.4 are injected into service sink I 7.1 respectively
Alkaline rinse, purified water, to meet the outer cleaning demand of the sampling probe 1.2 of sample sampling unit；The service sink II 7.2 into
Liquid mouth is connected by inlet tube II 7.6 with the first washing lotion bucket 2, and it is defeated that II 7.7(of delivery pump is in series on the inlet tube II 7.6
Sending pump II 7.7 is diaphragm pump), it is that service sink II 7.2 provides purified water by delivery pump II 7.7, meets reagent sampling unit and take
The outer cleaning demand of sample needle 1.2；Reagent altar 7.8(have refrigerating function, for containing reagent) condensate outlet, the magnetic bead
First leakage fluid dram II of the waste liquid port of cleaning unit waste liquid pool 7.9, the first leakage fluid dram I of service sink I 7.1 and service sink II 7.2
It is connected respectively by drain pipe I 7.10 with the import of the liquid trap 7.3, is in series on each drain pipe I 7.10
The outlet of 5th solenoid valve 7.11, liquid trap 7.3 is connected by sewer pipe 7.12 with 5 inlet tube 5.1 of waste liquid barrel, the waste liquid
It is diaphragm pump that I 7.13(positive displacement pump I 7.13 of positive displacement pump is in series on pipe 7.12).The drain of reagent altar 7.8 and magnetic bead cleaning unit
Process is identical, specifically by taking the drain of reagent altar 7.8 as an example: adjusting the 5th solenoid valve of control 7.8 drain pipe I 7.10 of reagent altar
7.11 are powered, and the condensate liquid in reagent altar 7.8 can be expelled in waste liquid barrel 5 by starting positive displacement pump I 7.13.
Sample sampling unit sampling probe 1.2 is identical with the outer cleaning process of reagent sampling unit sampling probe 1.2, specifically with sample
For this sampling unit: sampling probe 1.2 being moved in service sink I 7.1, the inlet tube I 7.4 of third washing lotion bucket 8 is connected to
Delivery pump I 7.5 persistently pumps alkaline rinse to service sink I 7.1；Adjust the drain pipe I 7.10 connecting with service sink I 7.1
The 5th solenoid valve 7.11 be powered, positive displacement pump I 7.13 continues working the cleaning waste liquid extracted in service sink I 7.1, until service sink
Without the waste liquid continuously flowed in I 7.1；Then the power-off of the 5th solenoid valve 7.11 is controlled, positive displacement pump I 7.13, which works on, to be located at
Drain pipe I 7.10 between 5th solenoid valve 7.11 and positive displacement pump I 7.13 is evacuated, and then adjusts the 5th solenoid valve 7.11 again
It is powered, moment under the action of negative pressure of the waste liquid in service sink I 7.1 enters drain pipe I 7.10 at this time, then again in positive displacement pump I
Enter in waste liquid barrel 5 under the action of 7.13, complete the alkaline rinse cleaning of sampling probe 1.2, negative pressure can ensure that waste liquid moment arranges
Out, the outer wall hanging liquid of sampling probe 1.2 is avoided completely；Aforesaid operations are repeated to clean sampling probe 1.2 with purified water.
To prevent liquid trap 7.3 and I 7.13 overload operation of positive displacement pump or waste liquid backflow phenomena occur, the service sink I
7.1 the second leakage fluid dram I and the second leakage fluid dram II of service sink II 7.2 are connected in parallel on the waste liquid by drain pipe II 7.14 respectively
On pipe 7.12, positive displacement pump II 7.15, the drain pipe II 7.14 of service sink I 7.1 are in series on each drain pipe II 7.14
On be in series with the 6th solenoid valve 7.16, it is ensured that the drain pipe II 7.14 is capable of forming vacuum zone in drain, it is ensured that sample sampling
The cleaning effect of unit sampling probe avoids hanging liquid；It is located at ullage, cleaning difficulty when the sampling probe sample-adding of reagent sampling unit
It is small, and the 5th solenoid valve 7.11 of service sink II 7.2 has met the cleaning demand of sampling below atmospheric pressure needle, because without in service sink
It separately connects on II 7.2 drain pipe II the 6th solenoid valve.
Liquid level sensor is provided in the waste liquid barrel 5, the first washing lotion bucket 2, the second washing lotion bucket 4 and third washing lotion bucket 8
9, monitored respectively by liquid level sensor waste liquid barrel 5 and the first washing lotion bucket 2, the second washing lotion bucket 4, third washing lotion bucket 8 liquid level simultaneously
The single-chip microcontroller of non-chemical luminescence immunoassay instrument is transmitted, once there is liquid level exception, abnormal signal is passed to analysis by unit machine
Alarm on instrument and alarm, remind staff, avoid liquid level abnormal and unpredictalbe risk caused by causing.
When work, the sampling probe 1.2 of sample sampling unit extracts quantitative sample in clean reaction cup, and sampling is completed
The cleaning pump 1.6 of sample sampling unit works afterwards, rinses sample sampling unit with the purified water in the first washing lotion bucket 2, cleaned
Start the positive displacement pump I 7.13 of waste unit in journey or the cleaning waste liquid in service sink I 7.1 is evacuated to waste liquid barrel by positive displacement pump II 7.15
In 5, the interior cleaning of sample sampling unit is completed；Then starting delivery pump I 7.5 first injected into service sink I 7.1 alkaline rinse into
Row alkali cleaning reinjects purified water cleaning, and the positive displacement pump I 7.13 of waste unit or positive displacement pump II 7.15 work in cleaning process, will
Cleaning waste liquid is evacuated in waste liquid bottle, and the outer cleaning for completing sample sampling unit sampling probe 1.2 keeps away in order to extract next sample
Exempt from cross contamination；Then reagent sampling unit successively adds reaction reagent, every addition one to the reaction cup for being loaded with sample respectively
Kind of reaction reagent carries out a cleaning action, completes sampling probe 1.2 first with the self-cleaning function of reagent sampling unit when cleaning
Interior cleaning, then purified water is injected into service sink II 7.2 using delivery pump II 7.7, to reagent sampling unit sampling probe 1.2
Outer cleaning is carried out, starts positive displacement pump I 7.13 or positive displacement pump II 7.15 in cleaning process and is evacuated to waste liquid is cleaned in waste liquid barrel 5, kept away
It is excused from an examination agent cross contamination；After sample and reaction reagent fully reacting, by the cleaning module of magnetic bead cleaning unit by the second washing lotion
Crystallinity cleaning solution in bucket 4 is injected into reaction and is cleaned, and will clean waste liquid by drawing liquid module after the completion of cleaning and extract
To waste liquid barrel 5, purified water is then recycled to clean cleaning module, then be evacuated to waste liquid barrel 5 for waste liquid is cleaned by drawing liquid module
It is interior, complete magnetic bead cleaning；A liquid (or B liquid) is injected into reaction cup eventually by substrate unit, the processing of sample is completed, by sample
Originally it send the detection module to chemiluminescent analyzer to carry out the detection that shines, improves the luminous detection success rate of sample.
Claims (10)
1. a kind of liquid channel system for chemical illumination immunity analysis instrument, including sample sampling unit, reagent sampling unit, substrate
Unit, magnetic bead cleaning unit and waste disposal unit, it is characterised in that: the magnetic bead cleaning unit includes cleaning module and drawing liquid
Module, the cleaning module include topping-up pump and the first washing lotion bucket, and the outlet end of the topping-up pump is communicated with multichannel liquid injection pipe
Road is in series with injection needle in each dispensing liquid pipe of the multichannel fluid injection pipeline and controls the of the injection needle reservoir quantity
One solenoid valve；Spaced series have second solenoid valve and flushing pump, position in flushing pipe between the topping-up pump and the first washing lotion bucket
It is in series with two-position three-way valve in the flushing pipe between the flushing pump and second solenoid valve, the of the two-position three-way valve
Three ends are connected by connecting tube with the second washing lotion bucket；The drawing liquid module includes the multichannel liquid suction pipe being connected with waste liquid barrel
Road, each of described multichannel drawing liquid pipeline, which divides, is in series with aspirating needle and suction pumps on liquid suction pipe.
2. the liquid channel system according to claim 1 for chemical illumination immunity analysis instrument, it is characterised in that: the substrate
Unit includes the identical substrate conveyor module of two structures, and the substrate conveyor module includes the bottom being connected by substrate pipeline
Object bottle and substrate needle are in series with substrate pump on the substrate pipeline, on the substrate pipeline of substrate pump two sides
It is in series with third solenoid valve.
3. the liquid channel system according to claim 1 for chemical illumination immunity analysis instrument, it is characterised in that: the sample
Sampling unit is identical with the reagent sampling unit structure, and sample sampling unit and reagent sampling unit include passing through collection tube
The sampling probe and sampling pump being connected, the sampling pump are connected by detergent line with the first washing lotion bucket, the cleaning
It is in series with the 4th solenoid valve and cleaning pump on pipeline, connects in the detergent line between the cleaning pump and the 4th solenoid valve
There is the isocon being connected with the first washing lotion bucket, is in series with first pressure sensor and overflow valve on the isocon.
4. the liquid channel system according to claim 3 for chemical illumination immunity analysis instrument, it is characterised in that: the sample
Second pressure sensor is in series on the collection tube of sampling unit.
5. the liquid channel system according to claim 1 for chemical illumination immunity analysis instrument, it is characterised in that: the waste liquid
Processing unit includes for cleaning the service sink I of sample sampling unit, for the service sink II of cleaning reagent sampling unit, liquid collecting
Device and the waste liquid barrel, the first drain of the condensate outlet of reagent altar, the waste liquid port of the magnetic bead cleaning unit, service sink I
First leakage fluid dram II of mouth I and service sink II is connected by drain pipe I with the import of the liquid trap respectively, each row
The 5th solenoid valve is in series in liquid pipe I, the outlet of liquid trap is connected with waste liquid barrel by sewer pipe, is gone here and there on the sewer pipe
It is associated with positive displacement pump I.
6. the liquid channel system according to claim 5 for chemical illumination immunity analysis instrument, it is characterised in that: the cleaning
Second leakage fluid dram I in pond I and the second leakage fluid dram II of service sink II are connected in parallel on the sewer pipe by drain pipe II respectively, often
It connects on a drain pipe II positive displacement pump II, is in series with the 6th solenoid valve on the drain pipe II of service sink I.
7. the liquid channel system according to claim 5 or 6 for chemical illumination immunity analysis instrument, it is characterised in that: described
Two inlets of service sink I are connected with the first washing lotion bucket, third washing lotion bucket respectively by inlet tube I, it is each described into
Delivery pump I is in series in liquid pipe I；The inlet of the service sink II is connected by inlet tube II with the first washing lotion bucket, institute
It states and is in series with delivery pump II on inlet tube II.
8. the liquid channel system according to claim 7 for chemical illumination immunity analysis instrument, it is characterised in that: the fluid injection
Pump, substrate pump and sampling pump are plunger pump；The flushing pump, cleaning pump, positive displacement pump I, positive displacement pump II, delivery pump I and conveying
Pump II is diaphragm pump；The suction pumps are peristaltic pump.
9. the liquid channel system according to claim 5 for chemical illumination immunity analysis instrument, it is characterised in that: the waste liquid
Liquid level sensor is provided in bucket, the first washing lotion bucket, the second washing lotion bucket and third washing lotion bucket.
10. the liquid channel system according to claim 3 for chemical illumination immunity analysis instrument, it is characterised in that: described to take
The outer surface of sample needle is provided with coated with Teflon protective layer.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920240032.7U CN209624623U (en) | 2019-02-26 | 2019-02-26 | Liquid channel system for chemical illumination immunity analysis instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920240032.7U CN209624623U (en) | 2019-02-26 | 2019-02-26 | Liquid channel system for chemical illumination immunity analysis instrument |
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| CN209624623U true CN209624623U (en) | 2019-11-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109709350A (en) * | 2019-02-26 | 2019-05-03 | 安图实验仪器(郑州)有限公司 | Liquid channel system for chemical illumination immunity analysis instrument |
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2019
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109709350A (en) * | 2019-02-26 | 2019-05-03 | 安图实验仪器(郑州)有限公司 | Liquid channel system for chemical illumination immunity analysis instrument |
| CN109709350B (en) * | 2019-02-26 | 2024-07-09 | 安图实验仪器(郑州)有限公司 | Liquid path system for chemiluminescent immunoassay analyzer |
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