CN208509955U - A kind of miniature save set frozen for human testicle and epididymal sperm - Google Patents
A kind of miniature save set frozen for human testicle and epididymal sperm Download PDFInfo
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- CN208509955U CN208509955U CN201820834160.XU CN201820834160U CN208509955U CN 208509955 U CN208509955 U CN 208509955U CN 201820834160 U CN201820834160 U CN 201820834160U CN 208509955 U CN208509955 U CN 208509955U
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Abstract
The utility model discloses a kind of miniature save sets frozen for human testicle and epididymal sperm, including straw, the side of the straw is plugged with tampon, the straw tampon end is connected with straw suction ball, the other end is connected with straw plug handle, the straw tampon end can also connect the heavy portion of straw plug, and the straw surface layer is coated with nontoxic temperature indicating agent, and outer wall has scale mark.The utility model is additionally arranged straw suction ball, easy to operate；Straw outer wall has scale mark, and convenient for the conversion of cryopreservation sperm sum, surface layer is coated with nontoxic temperature indicating agent, can be very good to monitor its cooling effect during the liquid nitrogen smoked of sperm steams；Straw plug handle one side design squarely, is easy to be affectedly bashful and carry out the label of patient information；The heavy portion of straw plug be metal material, avoid sample from floating in liquid nitrogen, and design have check valve, avoid thaw when air expansion cause bombing, while play the role of well it is closed, avoid the cross contamination in liquid nitrogen.
Description
Technical field
The utility model belong to medical instruments field more particularly to it is a kind of frozen for human testicle and epididymal sperm it is micro-
Type save set.
Background technique
The birth of intracytoplasmic sperm injection (ICSI) technology is the milestone of supplementary reproduction development, it solves part
The infertile problem as caused by male factor, the development of the technology and takes the improvement of precision method to irreversible obstructive nothing
Sperm patient brings Gospel.Currently, testis biopsy centesis (TESA), Percutaneous epididymal sperm aspiration (PESA) are no sperms
Disease patient effectively takes precision method.The patient of row TESA need to take vesical calculus position, be pierced after routine disinfection drape local anaesthesia by biopsy needle
Enter testis and obtain convoluted seminiferous tubule, the polished centrifuge washing of convoluted seminiferous tubule applies ware for ICSI injection, and the sperm in the source TESA is normal
Containing fragment of tissue, the spermatogenic cells at different stages etc. after haemocyte, grinding, impurity is more, mostly inactive sperm；Row PESA's
Patient then aspirates epididymis liquid with 20ML syringe, and ware is applied after centrifuge washing for ICSI injection, contained by the sperm in the source PESA
Impurity is less, slightly red blood cell, mostly motile sperm.The sperm quantity in the source TESA, PESA is limited and obtains process complexity, this
Some patientss medical history is relative complex, obtains the more rare more aobvious preciousness of sperm.With the development of Refrigeration Technique, in order to avoid taking ovum
The same day bridegroom's or husband's side takes essence failure or the repeatedly pain of operation puncturing, burden to the damage of organ-tissue and economically, epididymis,
The freezing of testicular sperm clinically becomes demand, but conventional Semen freezing technique is not particularly suited for epididymis, testicular sperm
Freezen protective, furthermore also without commercialization for epididymis testicular sperm freezing save set.
The micro device of frozen embryo includes open and two kinds of carrier of closure at present, freezing ring (cryloop) in
It is reported for the first time within 1999, for opening freezing storage device, which is the ring of diameter about 2mm made of a thin nylon yarn,
It is mounted in metallic rod, nylon yarn immerses after liquid since the effect of surface tension will form film, which can load embryo
It is freezed, but need to directly be contacted with liquid nitrogen, the risk of unavoidable pollution, clinically seldom used at present.
Crytop is the carrier of closure, and using extensive, it is a very thin and narrow laminar film, connect one it is hand-held
The small droplet of ovum or embryo and freezing liquid are loaded on film and immerse liquid nitrogen by bar, and band cap saves, in embryo vitrifying freeze
In it is more common, and anabiosis rate is good, it is to design for ovum and embryo, therefore useful load is smaller, about 2-3 microlitres, still
Volume after the sperm enrichment in the source TESA, PESA will be significantly larger than its useful load, therefore it is cold to be not used to Testes And Epididymides sperm
Freeze.
In clinical practice, conventional freezing of semen used carrier is mostly 2ML cell cryopreservation tube, and the carrier leakproofness is poor, low
Liquid nitrogen easily penetrates into and causes to pollute during temperature saves, the easy bombing of when defrosting, and stationarity is poor, easily overturns, and furthermore TESA, PESA are next
Source sperm usually only 0.2ml after centrifugal enrichment freezes, is put into after the cell cryopreservation tube of large volume that not only freezing rate is low,
Excessive air also increases the risk of bombing in pipe, while needing to be shifted sperm from cryopreservation tube with pipette or pipette tips after thawing
It to ICSI ware, is lost around here excessive, therefore limits the application of this method.
In presently disclosed patent document, Chinese patent CN1803100A, which is disclosed, freezes testis with empty egg vitellary membrane (ZP)
The method of ball sperm, the oolemma evacuated using endochylema are packed into oolemma as carrier, by the testicular sperm after biopsy.The preparation is empty
The method of oolemma is excessively cumbersome to be difficult to obtain (only discarded ovum, which is just looked, extracts empty oolemma), manpower economic cost mistake
Height, and the ethics problem that uses of discarded ovum sky oolemma also consider by band, therefore limits this method in clinical popularization and answer
With.
Chinese patent CN207185758U discloses a kind of obtained sperm pin type refrigerating plant of TESA, PESA, the sperm needle
Formula refrigerating plant and microscope work arm cooperate, and divide suction casing and memory paragraph, and the sealing of memory paragraph sperm is needed by high temperature, this
Damage to sperm in pipe be also it will be apparent that furthermore this skilled operation for needing micromanipulation system, technical requirements are higher,
It is not easy to popularize, often contains other cells and fragment of tissue after the enrichment of Testes And Epididymides Sperm treatment, and testicular sperm is mostly motionless essence
Son, it is difficult to screen, be taken long time with suction casing crawl sperm, manpower, technical costs are excessively high.
Straw is an a kind of plastics straw for tampon, has two kinds of specifications of 0.25ml and 0.5ml, 0.25ml is mainly used for
Sequencing freezes embryo at a slow speed, and detailed directions are will to freeze after the micro liquid tubulature for containing embryo, but the straw is without absorption liquid
Corollary apparatus, it is inconvenient for use, as the development of vitrification has seldom used；0.5ml to be usually used in vitrifying cold
Freeze, the load bar of the film carrier equipped with similar Crytop, bar insertion straw will be carried after film on embryo's loading and put into Liquid nitrogen storage,
The load volume of same slide glass is too small, which is unsuitable for the sperm freezing of Testes And Epididymides sperm source.
Utility model content
Purpose of utility model: the utility model aim is to be unsuitable for Testes And Epididymides sperm freezing for existing straw, is such as grasped
Make the problems such as inconvenient, useful load is small, provide a kind of structure be simple and convenient to operate, freezing epididymis easy to produce, testicular sperm
Straw.
Technical solution: the utility model includes straw, and the side of the straw is plugged with tampon, and the straw tampon end connects
It is connected to straw suction ball, the other end is connected with straw plug handle, and the straw tampon end can also connect the heavy portion of straw plug, institute
The straw surface layer stated is coated with nontoxic temperature indicating agent, and outer wall has graduation mark.
The straw plug handle is arc on one side, is on one side rectangle, is connect, is convenient for straw by handle joint lever
It is affectedly bashful and carries out the label of patient information.
The handle joint lever is solid tubes, and outer diameter is consistent with straw internal diameter.
The straw suction ball connect by connecting rod with straw, and the connecting rod is hollow rod, outer diameter in straw
Diameter is consistent, and straw suction ball is arranged, and avoids existing straw without proprietary suction device bring is inconvenient and the risks such as pollution sources.
The volume of the straw suction ball is 0.25ml, to there is enough suction to fill straw.
The heavy portion of straw plug uses metal material, and outer diameter is consistent with straw internal diameter, avoids sample in liquid nitrogen
Floating.
The straw plug is equipped with check valve among heavy portion, and air expansion causes bombing when avoiding thawing, and plays simultaneously
Good closed effect, avoids the cross contamination in liquid nitrogen.
The utility model has the advantages that the utility model is additionally arranged straw suction ball, existing straw is avoided without proprietary suction device bring
Inconvenient and pollution sources risks；Straw outer wall has scale mark, the volume that operator can be prompted to freeze, convenient for freezing
Deposit the conversion of sperm sum；Straw surface layer is coated with nontoxic temperature indicating agent, can be very good to monitor during the liquid nitrogen smoked of sperm steams
Its cooling effect；Straw plug handle one side design squarely, is easy to be affectedly bashful and carry out the label of patient information；The heavy portion of straw plug
For metal material, sample is avoided to float in liquid nitrogen, and design has check valve, air expansion causes bombing when avoiding thawing, together
When play the role of well it is closed, avoid the cross contamination in liquid nitrogen.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model；
Fig. 2 is the straw suction ball schematic diagram of the utility model；
Fig. 3 is the straw plug handle schematic diagram of the utility model；
Fig. 4 is the heavy portion's schematic diagram of straw plug of the utility model.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawing.
As shown in Figures 1 to 4, the utility model includes straw 1, tampon 2, graduation mark 3, connecting rod 4, straw suction ball 5, handle
Connecting rod 6, straw plug handle 7, the heavy portion tail portion 8 of straw plug, the heavy size portion 9 of straw plug and check valve 10.The side of straw 1
It is plugged with the tampon 2 of long 50mm, 1 tampon end of straw is connected with straw suction ball 5, avoids existing straw and bring without proprietary suction device
The risks such as inconvenient and pollution sources；The other end is connected with straw plug handle 7, and 1 tampon end of straw can also connect straw plug
Heavy portion.The outer diameter of straw 1 is 1mm, and internal diameter 0.8mm, overall length 10cm, surface is coated with low temperature resistant temperature indicating coating, in sperm
Liquid nitrogen smoked can be very good to monitor its cooling effect during steaming, and outer wall has graduation mark 3, operator can be prompted to freeze
Volume, convenient for the conversion of cryopreservation sperm sum.
Straw suction ball 5 is connect by connecting rod 4 with straw 1, and connecting rod 4 is hollow rod, and outer diameter is consistent with 1 internal diameter of straw to be
0.8mm, long 1cm, 5 volume of straw suction ball is 0.25ml, to there is enough suction to fill straw 1.Straw suction ball 5 is set,
It can be to avoid existing straw without proprietary suction device bring is inconvenient and the risks such as pollution sources.
7 one side of straw plug handle is arc, is on one side rectangle, is connect by handle joint lever 6 with straw 1, convenient for taking
Pinch and carry out the label of patient information.Handle joint lever 6 is solid tubes, and outer diameter consistent with 1 internal diameter of straw is 0.8mm, long
1cm, straw plug handle 7 long 2cm, diameter 4mm.
The heavy portion of straw plug includes the heavy portion tail portion 8 of straw plug, the heavy size portion 9 of straw plug and check valve 10, straw plug
The diameter in heavy size portion 9 is greater than the heavy portion tail portion 8 of straw plug.The heavy portion of straw plug uses metal material, avoids sample in liquid
It is floated in nitrogen, wherein the outer diameter of the heavy portion tail portion 8 of straw plug is consistent with the internal diameter of straw 1.The heavy portion tail portion 8 of straw plug is long
50mm, diameter 0.8mm, the diameter in the heavy size portion 9 of straw plug are 4mm, check valve 10 are equipped among the heavy portion of straw plug, only
Straw 1 is allowed to be vented from inside to outside, liquid nitrogen cannot be introduced into straw 1 after being placed in liquid nitrogen container, and air expansion causes fried when avoiding thawing
Pipe, at the same play the role of well it is closed, avoid the cross contamination in liquid nitrogen.
Specific operation process are as follows:
1) it prepares sperm sample: patient information is marked before freezing first at straw plug handle 7, such as name, medical record number, essence
Sub- source etc., the source TESA sperm are enriched with after need to grinding washing centrifugation, the direct centrifuge washing enrichment of the source PESA sperm, after enrichment
Sperm 0.1ml culture solution is added, while isometric freezing liquid is added and forms sperm sample, the sample after mixing is put in room temperature
Set 10min；
2) tubulature: squeezing straw suction ball 5, while connecting rod 4 being inserted in the tampon end of straw 1, and the other end of straw 1 is immersed
Empty culture medium gently unclamps straw suction ball 5, slowly 20 microlitres of sucking empty culture mediums, and the nozzle of straw 1 is detached from liquid level, sucking
20 microlitres of air columns, then straw 1 is immersed in the required sperm sample freezed, gently unclamp straw suction ball 5, it is seen that sperm sample
Straw 1 is slowly sucked, pays close attention to the sample volume of suction casing, about 20 microlitres (specific volume can be adjusted according to sperm situation), then
One section of 20 microlitres of air column is sucked, one section 20 microlitres empty culture mediums is sucked again later, continues to unclamp straw suction ball 5, until first
The empty culture medium that step is drawn soaks tampon 2, and tubulature at this time is completed；
3) straw seals: handle joint lever 6 is filled in straw 1 and screwed by hand-held straw plug handle 7, by straw suction ball 5 from
The removal of 1 tampon end of straw, while the heavy portion tail portion 8 of straw plug is screwed in into 1 tampon end of straw；
4) Liquid nitrogen storage: straw 1 being put on liquid nitrogen box and fumigates 30min, pays close attention to the variation of 1 skin color of straw simultaneously at this time
The temperature-fall period of monitor sample is recorded, investment liquid nitrogen container saves.
Claims (7)
1. a kind of miniature save set frozen for human testicle and epididymal sperm, including straw (1), the straw (1)
Side is plugged with tampon (2), which is characterized in that the tampon end of the straw (1) is connected with straw suction ball (5), and the other end is connected with
Straw plug handle (7), the tampon end of the straw (1) can also connect the heavy portion of straw plug, and the straw (1) surface layer is coated with
Nontoxic temperature indicating agent, outer wall have graduation mark (3).
2. a kind of miniature save set frozen for human testicle and epididymal sperm according to claim 1, feature
It is, it is on one side rectangle that the straw plug handle (7), which is on one side arc, even by handle joint lever (6) and straw (1)
It connects.
3. a kind of miniature save set frozen for human testicle and epididymal sperm according to claim 2, feature
It is, the handle joint lever (6) is solid tubes, and outer diameter is consistent with straw (1) internal diameter.
4. a kind of miniature save set frozen for human testicle and epididymal sperm according to claim 1, feature
It is, the straw suction ball (5) is connect by connecting rod (4) with straw (1), and the connecting rod (4) is hollow rod, outer diameter
It is consistent with straw (1) internal diameter.
5. a kind of miniature save set frozen for human testicle and epididymal sperm according to claim 1 or 4, special
Sign is that the volume of the straw suction ball (5) is 0.25ml.
6. a kind of miniature save set frozen for human testicle and epididymal sperm according to claim 1, feature
It is, the heavy portion of straw plug uses metal material, and outer diameter is consistent with straw (1) internal diameter.
7. a kind of miniature save set frozen for human testicle and epididymal sperm according to claim 1 or 6, special
Sign is that the straw plug is equipped with check valve (10) among heavy portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820834160.XU CN208509955U (en) | 2018-05-31 | 2018-05-31 | A kind of miniature save set frozen for human testicle and epididymal sperm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820834160.XU CN208509955U (en) | 2018-05-31 | 2018-05-31 | A kind of miniature save set frozen for human testicle and epididymal sperm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208509955U true CN208509955U (en) | 2019-02-19 |
Family
ID=65340704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820834160.XU Expired - Fee Related CN208509955U (en) | 2018-05-31 | 2018-05-31 | A kind of miniature save set frozen for human testicle and epididymal sperm |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208509955U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111194743A (en) * | 2020-02-25 | 2020-05-26 | 黄吴键 | Sperm freezing carrier system and ultra-fast sperm freezing method |
| CN112544610A (en) * | 2020-11-23 | 2021-03-26 | 杭州师范大学 | In-vitro conservation method of mouse epididymis |
| CN112674076A (en) * | 2021-01-08 | 2021-04-20 | 创健医疗(深圳)有限公司 | Closed freezing pole that carries |
| CN113303324A (en) * | 2021-04-29 | 2021-08-27 | 江西康雅医疗用品有限公司 | Fully-closed quick-freezing vitrification cryopreservation tube |
-
2018
- 2018-05-31 CN CN201820834160.XU patent/CN208509955U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111194743A (en) * | 2020-02-25 | 2020-05-26 | 黄吴键 | Sperm freezing carrier system and ultra-fast sperm freezing method |
| CN112544610A (en) * | 2020-11-23 | 2021-03-26 | 杭州师范大学 | In-vitro conservation method of mouse epididymis |
| CN112674076A (en) * | 2021-01-08 | 2021-04-20 | 创健医疗(深圳)有限公司 | Closed freezing pole that carries |
| CN113303324A (en) * | 2021-04-29 | 2021-08-27 | 江西康雅医疗用品有限公司 | Fully-closed quick-freezing vitrification cryopreservation tube |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190219 |