CN201282994Y - Device for absorbing and storing trace amounts of biological substance - Google Patents
Device for absorbing and storing trace amounts of biological substance Download PDFInfo
- Publication number
- CN201282994Y CN201282994Y CNU2008201342133U CN200820134213U CN201282994Y CN 201282994 Y CN201282994 Y CN 201282994Y CN U2008201342133 U CNU2008201342133 U CN U2008201342133U CN 200820134213 U CN200820134213 U CN 200820134213U CN 201282994 Y CN201282994 Y CN 201282994Y
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- pipe
- biological substance
- outer tube
- suction
- interior pipe
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Abstract
The utility model relates to a micro-extracting reservoir for biological substance, which comprises an inner tube (1), an outer tube (3), a sucker (2), a hand-control syringe (4) and latex tubes (5), wherein the lower end of the inner tube (1) is inserted into the outer tube (3), the upper end of the inner tube (1) is connected with the sucker (2) through one latex tube (5), and the hand-control syringe (4) is connected with an opening at an butt end of the outer tube (3) through one latex tube (5). The micro-extracting reservoir can transfer or freeze and store biological substance with minute quantity of liquid, and has the advantages of accurate control and safe and convenient use.
Description
Technical field
This utility model relates to a kind of biological substance minim suction storer, is specifically related to a kind of minim suction storer that can accurately shift biological substance.
Background technology
Along with the development of biological Refrigeration Technique, increasing biological substance can be preserved for a long time, and providing as the preservation that develops into human surplus capacity of the frozen technology of people's ovum may; Ovary tissue save as those since a variety of causes must ovariectomized women bring Gospel.This a series of achievement gives the credit to the improvement of freezing culture fluid prescription to a great extent, but certain facilitation has also been played in the improvement of frozen matter carrier and relevant aspiration technique.
Research and improvement through many decades; the slow freezing technology is relatively ripe; demanding urgently breaking through but there is also very severe bottleneck, is exactly the formation of ice crystal in the slow freezing process and the infiltration of cryoprotective agent toxicity to the particularly damage of embryo and oocyte of biological substance.Glass freezing is as a kind of emerging Refrigeration Technique, and the simplicity of its operation and higher success rate more and more are subjected to people's favor.The carrier that is used for the biological substance glass freezing at present has multiple, as Cryoloop, Cryoleaf, electron microscopic grid etc.On using, these carriers have all been obtained good refrigerating effect.The accurate absorption to sample is depended in the acquisition of these achievements, because it is closely related to follow sample to enter the amount of liquid and the refrigerating effect of carrier together, this also is a refrigerating effect because of one of people's proficiency level and the different reasons that there are differences of performance state.
At present used glass freezing cell extractor is mainly Pasteur dropper or the capillary tube that draws through alcohol burner, inevitably a large amount of liquid can be followed sample together in the suction tube when drawing biological substance, has brought inconvenience to next step operation.
Summary of the invention
At above technological deficiency, this utility model provide a kind of safe in utilization easily, control accurate biological substance minim suction storer.
Pipe 1, outer tube 3, mouthful suction pipe 2, manual control injector 4 and latex tubing 5 in this utility model biological substance minim suction storer comprises; Wherein, outer tube 3 inside are stretched in described interior pipe 1 lower end, and interior pipe 1 upper end is connected with a mouthful suction pipe 2 by latex tubing 5, and manual control injector 4 is connected with outer tube 3 butt end openings by latex tubing 5.
In this utility model, the spout diameter of described interior pipe 1 lower end of stretching into outer tube 3 is bigger 10～40 microns than the diameter that will draw biological substance, thus the amount of trace control is inhaled biological substance and liquid; The mouth of pipe external diameter of pipe 1 lower end in the spout diameter of its outer tube 3 thin end parts is a bit larger tham, and mouth of pipe external diameter and biological substance twice external diameter sum less than interior pipe 1 lower end, mainly in order to the gathering of control main body biological substance, the pipe thickness of wherein interior pipe 1 lower end is the 1-50 micron.
In this utility model, described manual control injector 4 is the ordinary syringe of this area routine, includes but not limited to for example microcontroller syringe of 1ml, 5ml syringe or this area routine, includes but not limited to for example Narishige, eppendorf syringe etc.
In this utility model, described mouthful suction pipe 2 be mouthful suction pipe of this area routine, as extensive stock mouth suction pipe, and also can be for containing the self-control mouth suction pipe of forming by filter, flexible pipe of filter.
In this utility model, the making material of described interior pipe 1, outer tube 3 is transparent, the anti-ultralow temperature of this area routine or the material with high thermal conductivity, includes but not limited to, for example poly-fluorine pipe, Merlon pipe.
In this utility model, described biological substance is all kinds cell, embryo, piece of tissue or small-sized bion, and wherein the diameter of this biological substance is 10～1000 microns.
This utility model minim suction storer in use, at first through port suction pipe 2 is drawn certain glass freezing liquid in interior pipe 1, the interior pipe 1 that will contain a certain amount of freezing liquid embeds among the outer tube 3, seals air tight in guaranteeing between pipe 1 and the outer tube 3; Regulate the manual control injector 4 that links to each other with outer tube 3 then, the biological substance that is transferred in advance in the freezing liquid is sucked in the outer tube 3 together with an amount of freezing liquid, when freezing liquid enters outer tube 3, pipe 1 lower ending opening place in biological substance is blocked in; Slightly apply a little negative pressure by a mouth suction pipe 2 that links to each other with interior pipe 1 this moment, pipe 1 thin position in freezing liquid that just can will required biological substance and minute quantity sucks, and this part contains one section air column of existence between the freezing liquid of the liquid of biological substance and absorption in advance; Pipe 1 in extracting then carries out freezing getting final product with the biological substance taking-up, or carries out other operation; Pipe 1 also can be directly as freezing preservation pipe in of the present utility model.
In this utility model, those skilled in the art can select interior pipe, the outer tube of different materials and different inner diameters according to different biological substances, thereby select to be suitable for the utility model of the present invention of this biological substance.
Characteristics simple to operate, safe ready that this utility model minim suction storer has, and can accurately control the amount of following biological substance to enter the liquid in the carrier, improved refrigerating effect.
Description of drawings
Fig. 1 is the structural representation of biological substance minim suction storer;
Fig. 2 is the user mode figure of biological substance minim suction storer.
The specific embodiment
Below in conjunction with drawings and Examples this utility model is further described, but this utility model is not limited to this.
As shown in Figure 1, this utility model biological substance minim suction storer, pipe 1, outer tube 3, mouthful suction pipe 2, manual control injector 4 and latex tubing 5 in comprising; Wherein, interior pipe 1 lower end is stretched in the outer tube 3, interior pipe 1 upper end is connected with a mouthful suction pipe 2 by latex tubing 5, manual control injector 4 is connected with outer tube 3 butt end openings by latex tubing 5, the spout diameter of interior pipe 1 lower end that stretches into outer tube 3 is bigger 10～40 microns than the diameter that will draw biological substance, and wherein the diameter of this biological substance is 10～1000 microns; The mouth of pipe external diameter of pipe 1 lower end in outer tube 3 spout diameters are a bit larger tham, and less than mouth of pipe external diameter and the biological substance twice external diameter sum of interior pipe 1 lower end, the pipe thickness of managing 1 lower end in wherein is the 1-50 micron.The user mode of this utility model biological substance minim suction storer as shown in Figure 2, wherein 6 among Fig. 2 is that freezing liquid, 7 is biological substance and a small amount of freezing liquid for air column, 9 for biological substance and an amount of freezing liquid, 8.
The absorption of embodiment 1 human oocyte:
(1), makes the outer tube 3 of external diameter 200, internal diameter 180 of glass material with the interior pipe 1 of polyethylene tube making external diameter 150, internal diameter 130;
(2) draw certain vitrified freezing liquid 6 by homemade mouthful of suction pipe 2 in interior pipe 1, (composition is freezing liquid 6: improvement HOF (mHTF)+20% serum substitute (sss)+15% ethylene glycol (EG)+15% dimethyl sulfoxide (DMSO)+0.5M sucrose (Sucrose));
(3) will contain among the interior pipe 1 embedding outer tube 3 of a certain amount of freezing liquid 6, seal air tight in guaranteeing between pipe 1 and the outer tube 3;
(4) 2～3 pieces of human oocytes are handled 4～5 minutes in composition is the liquid of mHTF+20%SSS+7.5%EG+7.5%DMSO after, be transferred in the final freezing liquid;
(5) negative pressure in the manual control injector 4 of the common 1ml that adjusting links to each other with outer tube 3 after the half point kind enters among the outer tube 3 oocyte and an amount of freezing liquid 7 together;
When (6) freezing liquid enters outer tube 3,1 mouthful of place of pipe in oocyte is blocked in, this moment by the mouth suction pipe 2 that links to each other with interior pipe 1 slightly apply a little negative pressure just the freezing liquid 9 of oocyte and minute quantity can be sucked in pipe 1 thin position, this part contains one section air column 8 of existence between the freezing liquid of the liquid of oocyte and absorption in advance;
(7) extract in pipe 1, the freezing liquid 9 of oocyte and minute quantity is placed on the Cryoloop, immerse liquid nitrogen again and carry out freezing preservation.
Embodiment 2: the absorption of people 8-cell stage
(1), makes the outer tube 3 of external diameter 200, internal diameter 180 of glass material with the interior pipe 1 of polyethylene tube making external diameter 150, internal diameter 130;
(2) draw certain glass freezing liquid by commercial mouthful of suction pipe 2 in interior pipe 1, the freezing liquid composition is mHTF+20%sss+15%EG+15%PROH+0.5M Sucrose;
(3) will contain among the interior pipe 1 embedding outer tube 3 of a certain amount of freezing liquid, seal air tight in guaranteeing between pipe 1 and the outer tube 3;
(4) 2～3 pieces of people 8-cell stages being put into composition is after the liquid of mHTF+20%SSS+7.5%EG+7.5%PROH is handled 4～5 minutes, to be transferred in the final freezing liquid;
(5) regulate after the half point kind with spiral syringe that outer tube 3 links to each other in negative pressure, the embryo is entered among the outer tube 3 together with an amount of freezing liquid;
When (6) freezing liquid enters outer tube 3,1 mouthful of place of pipe in the embryo is blocked in, this moment by the mouth suction pipe 2 that links to each other with interior pipe 1 slightly apply a little negative pressure just the freezing liquid of embryo and minute quantity can be sucked in pipe 1 thin position, this part contains one section air column of existence between the freezing liquid of embryo's liquid and absorption in advance;
(7) extract interior pipe 1, embryo and a spot of freezing liquid are placed on the Cryoloop, immerse liquid nitrogen again and carry out freezing preservation.
Embodiment 3: cattle MII phase bovine oocyte embryo's absorption
(1), makes the outer tube 3 of external diameter 220, internal diameter 200 of glass material with the interior pipe 1 of polyethylene tube making external diameter 180, internal diameter 150;
(2) draw certain micrurgy liquid by homemade mouthful of suction pipe 2 at interior pipe 1, operation liquid composition is G-MOPs+5mg/mlBSA;
(3) will contain among the interior pipe 1 embedding outer tube 3 of a certain amount of operation liquid, seal air tight in guaranteeing between pipe 1 and the outer tube 3;
(4) regulate negative pressure in the common 1ml syringe link to each other with outer tube 3, make 10～20 pieces of cattle MII phase bovine oocyte embryos enter in the outer tube 3 culture fluid composition G1.5+5mg/mlBSA together with an amount of culture fluid;
When (5) culture fluid enters outer tube 3,1 mouthful of place of pipe in the embryo is blocked in, this moment by the mouth suction pipe 2 that links to each other with interior pipe 1 slightly apply a little negative pressure just the culture fluid liquid of cattle ovum and minute quantity can be sucked in pipe 1 thin position, this part contains one section air column of existence between the operation liquid of embryo's liquid and absorption in advance;
(6) extract in pipe 1, cattle ovum and a spot of culture fluid be blown into contain in the nuclear transplantation ware of operate liquid, and carry out nuclear transplantation and operate.
Embodiment 4: the storage of human oocyte
(1) outer tube 3 of the interior pipe 1 of making external diameter 150, internal diameter 130 and external diameter 200, internal diameter 180;
(2) draw certain final freezing liquid with interior pipe 1, the freezing liquid composition is mHTF+20%sss+15%EG+15%DMSO+0.5M Sucrose;
(3) will contain among the interior pipe 1 embedding outer tube 3 of a certain amount of freezing liquid, seal air tight in guaranteeing between pipe 1 and the outer tube 3;
(4) with 2-3 piece of human oocyte after composition is to handle 4-5 minute in the liquid of mHTF+20%SSS+7.5%EG+7.5%DMSO, be transferred in the final freezing liquid;
(5) regulate after the half point kind with syringe that outer tube 3 links to each other in negative pressure, oocyte is entered among the outer tube 3 together with an amount of freezing liquid;
When (6) freezing liquid enters outer tube 3,1 mouthful of place of pipe in oocyte is blocked in, this moment by the mouth suction pipe 2 (Fig. 1-2) that links to each other with interior pipe 1 slightly execute a little negative pressure just the freezing liquid of oocyte and minute quantity can be sucked in pipe 1 thin position, this part contains one section air column of existence between the freezing liquid of the liquid column of oocyte and absorption in advance;
(7) extract in pipe 1, the liquid column that will contain oocyte makes its segment distance that rises by negative pressure, thereby and use interior pipe 1 opening part to be clutched and make its sealing through alcohol burner disinfectant tweezers;
(8) subsequently, interior pipe 1 elongated portion that will contain oocyte immediately immerses in the liquid nitrogen, removes mouthful suction pipe 2 and the other end of tubule is clogged with stopper, and preserve in the pipe 1 immersion liquid nitrogen in will be whole.
Claims (6)
1. biological substance minim suction storer, it is characterized in that, described reservoir comprises interior pipe (1), outer tube (3), mouthful suction pipe (2), manual control injector (4) and latex tubing (5), outer tube (3) inside is stretched in pipe (1) lower end in described, interior pipe (1) upper end is connected with a mouthful suction pipe (2) by latex tubing (5), and manual control injector (4) is passed through latex tubing (5) and is connected with outer tube (3) butt end opening.
2. biological substance suction storer according to claim 1 is characterized in that, the spout diameter of described interior pipe (1) lower end is bigger 10～40 microns than the diameter that will draw biological substance, and wherein the diameter of biological substance is 10～1000 microns.
3. biological substance suction storer according to claim 2, it is characterized in that, the spout diameter of the thin end of described outer tube (3) is a bit larger tham the mouth of pipe external diameter of interior pipe (1) lower end, and less than the mouth of pipe external diameter and the biological substance twice external diameter sum of interior pipe (1) lower end, the pipe thickness of wherein interior pipe (1) lower end is the 1-50 micron.
4. biological substance suction storer according to claim 3 is characterized in that, described manual control injector (4) is ordinary syringe or microcontroller syringe.
5. biological substance suction storer according to claim 4 is characterized in that, described mouthful of suction pipe (2) is for containing the self-control mouth suction pipe or the commercialization mouth suction pipe of filter.
6. biological substance suction storer according to claim 5 is characterized in that, described interior pipe (1), outer tube (3) are for having the material of transparent, anti-ultralow temperature or high thermal conductivity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201342133U CN201282994Y (en) | 2008-09-12 | 2008-09-12 | Device for absorbing and storing trace amounts of biological substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201342133U CN201282994Y (en) | 2008-09-12 | 2008-09-12 | Device for absorbing and storing trace amounts of biological substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201282994Y true CN201282994Y (en) | 2009-08-05 |
Family
ID=40947978
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201342133U Expired - Lifetime CN201282994Y (en) | 2008-09-12 | 2008-09-12 | Device for absorbing and storing trace amounts of biological substance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201282994Y (en) |
-
2008
- 2008-09-12 CN CNU2008201342133U patent/CN201282994Y/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Shandong Shanda Affiliated Reproduction Hospital Co.,Ltd. Assignor: Chen Zijiang Contract record no.: 2010370000563 Denomination of utility model: Device for absorbing and storing trace amounts of biological substance Granted publication date: 20090805 License type: Exclusive License Record date: 20101210 |
|
| CX01 | Expiry of patent term |
Granted publication date: 20090805 |
|
| CX01 | Expiry of patent term |