CN206949363U - It is a kind of to be applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum - Google Patents
It is a kind of to be applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum Download PDFInfo
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- CN206949363U CN206949363U CN201720765038.7U CN201720765038U CN206949363U CN 206949363 U CN206949363 U CN 206949363U CN 201720765038 U CN201720765038 U CN 201720765038U CN 206949363 U CN206949363 U CN 206949363U
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Abstract
The utility model is related to it is a kind of be applied to the freezing mankind's rareness sperm and closed carrier of the double flute of embryo and ovum, be made up of carrier panel, double flute loading part and operation handle.Carrier panel is a head on both sides chamfering, the middle plate for being provided with the cylindrical hole run through, and afterbody connects with the head of double flute loading part.Double flute loading part is the assembly that cuboid is cut into, and both sides respectively cut off an arcuate stream line body, and both side surface respectively sets a loading chute, and loading chute head end opening afterbody joint face tilts upward.Double flute loading part afterbody connects with operation handle, and operation handle is a cuboid.Its advantage is shown：Rare sperm can be freezed, and can retains frozen embryo or ovum, rare sperm is loaded in double flute useful load up to 20 microlitres, embryo or ovum can be loaded in leading edge aperture, enclosed effect can be formed during freezing, because carrier lengths are shorter, double-deck storage can be taken when freezing, increases the storage quantity in liquid nitrogen container.
Description
Technical field
Technical field of medical instruments is the utility model is related to, is that one kind is applied to freezing mankind's rareness sperm specifically
And the closed carrier of the double flute of embryo and ovum.
Background technology
The ratio of male sterility patient increasingly increases in recent years, and clinical manifestation is few smart, weak smart, different without sperm qualities such as essences
Often.Aspermia or oligospermia patient can obtain micro sperm by ejaculating, and azoospermia patients can pass through testicalar sperm aspiration
(TESA) and Percutaneous epididymal sperm aspiration (PESA) obtains a small amount of testis or epididymal sperm, then passes through mankind's monosperm ovum
Endochylema injection (ICSI) technology can make wife's successful pregnancies of these patients.
These sperm quantities are limited and hard-earned, it is therefore necessary to by rare sperm cryopreservation.For serious few
Smart disease patient, freeze essence can avoid taking the ovum same day because no sperm and caused by fertilization failure risk；For patients with azoospermia, freeze
Essence can be avoided because operation puncturing takes the smart body brought to patient, mentally pain repeatedly.
The method that traditional 2 milliliters of spermatozoa cryopreservation pipes of use freeze essence can cause the different degrees of loss of frozen-thawed spermatozoa, be not suitable for
The freezing of rare sperm.It is one of research emphasis of current rare sperm freezing to find suitable freezing carrier.
The rare sperm freezing carrier reported both at home and abroad at present mainly includes：Empty egg vitellary membrane (Zona
Pellucida ring (Cryoloop), cryovial (Cryolock) and freezing film (Cryotop) etc.), are freezed.Empty ovum is transparent
The problem of band is because of its biological safety, can not clinically be promoted；And Cryoloop, Cryolock, Cryotop are embryos
The freezing carrier of tire, the frozen liq sample of 5 microlitres of underrun is only capable of, clinically the volume of fluid of rare sperm is generally
20~30 microlitres, isometric cryoprotector is also added during freezing, therefore can not also meet the needs of clinical.
This research group early stage on the basis of thin-film carrier Cryotop, by lengthening overstriking its film loading part, passes through
Clinical trial, the freezing of rare sperm is efficiently solved the problems, such as, while also remain frozen embryo and the function of ovum, successfully obtained
Obtained the mandate (201520231461.X) of a china national practical new-type patent.This new support is named as Cryoplus by us.
But Cryoplus is as Cryotop, is all a kind of open carrier, i.e., in freezing freeze sample (sperm,
Ovum or embryo) directly it can all be contacted with liquid nitrogen or liquid nitrogen vapor.There are some researches show each in the environment of subzero 196 degree of liquid nitrogen
Kind malignant bacteria is appointed with virus to survive, therefore open carrier exists by the possibility of pathogenic microorganisms pollution in liquid nitrogen in theory
Property, U.S. FDA also no longer ratifies the listing of open carrier in recent years.And closed carrier, in the mistake for freezing and thawing
Sample and liquid nitrogen or liquid nitrogen vapor are freezed in journey can accomplish physical isolation, thus be safe.So exploitation is a kind of both at present
Clinically rare sperm freezing can be met, and can retains frozen embryo and the closed freezing carrier of ovum function is necessary.
Chinese patent literature：CN104823966A disclose a kind of micro sperm cryopreservation carrier and corresponding freezing and
Defreezing method, freezen protective carrier is rectangle, square or circular horizontal or groove-like, and size is individual for that can carry 1-3
0.1-10.0uL freezing liquid droplet, made with transparent plastic material or transparent glass material.It is accurate on freezen protective carrier
Insertion is covered in the sperm culture dish of oil after getting freezing liquid droplet ready, is inserted freezing liquid droplet with injection needle crawl sperm, is taken
Go out freezen protective carrier and blot mineral oil with blotting paper, snap frozen, freezen protective carrier is inserted directly into ICSI during defrosting
In ware, its 37 DEG C of mineral oil recovery sperms are utilized.Chinese patent literature：It is dilute that CN205794638U discloses a kind of spiral cover bushing type
Few sperm superthin section freezing carrier, including outer tube, freezing slide glass, knob lid；Described freezing slide glass one end is fixed on knob
Cover, in other end insertion outer tube；Described overcoat tube end connection knob lid；Described outer tube one end is provided with external screw thread；
Described knob lid includes sub- knob lid and female knob lid；Described sub- knob lid and the medial surface of female knob lid are equipped with interior spiral shell
Line；Described sub- knob outer side of lid is provided with son and latched；Locking device and a female is provided with the outside of described female knob；Described sub- knob covers also
Provided with platform, and platform is provided with fixed groove；One end of the slide glass is additionally provided with circular indicia；The other end of the slide glass is also
Provided with fixing strip, handle；Described fixing strip is engaged with fixed groove.Chinese patent literature：CN203290151U is public
A kind of rare sperm novel closed-type is opened and has freezed ultra-fine pipe carrier, provided with inner tube, described inner tube is provided with butt end and taper end, institute
The interior tube outside stated is provided with outer tube, and inner tube and outer tube relative can slide, and the length of described inner tube is more than outer tube
Length.But it is applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum on of the present utility model
It yet there are no report.
The content of the invention
The purpose of this utility model is to provide a kind of suitable for freezing mankind's rareness sperm and the double flute of embryo and ovum
Closed carrier.
To achieve the above object, the utility model adopts the technical scheme that：One kind is applied to freezing mankind's rareness sperm
And the closed carrier of the double flute of embryo and ovum, the closed carrier of described double flute is by carrier panel, double flute loading part and behaviour
Make handle composition；Described carrier panel is the plate of both sides chamfering, centre provided with the cylindrical hole run through, institute above and below a head
The afterbody for the carrier panel stated connects with the head of double flute loading part；Described double flute loading part is the combination that cuboid is cut into
Body, described double flute loading part head each side cut off an arcuate stream line body, and left and right sides surface respectively sets a dress
Carry groove；Described double flute loading part afterbody connects with operation handle, and described operation handle is a cuboid；Described carrier
3~6mm of length, the wide 2~2.3mm of panel, a diameter of 0~1.5mm of described cylindrical hole, described cylindrical hole is apart from carrier
0.5~2mm of face plate edge, it is wide that described double flute loads minister's loading chute groove face of 25~35mm, wide 2~2.3mm, described two
1.2~1.6mm, 0.8~1mm of groove depth, described operation handle length 35~45mm, wide 2~2.3mm, high 2~2.3mm.
The closed carrier of described double flute also includes 0.25ml straw.
The utility model advantage is：
1st, the closed carrier of double flute of the present utility model can freeze rare sperm, and and can retains frozen embryo or ovum.
2nd, during carrier closed using double flute of the present utility model, rare sperm is loaded in double flute, and useful load is up to 20
Microlitre, embryo or ovum can protect agent with freezing and be loaded in leading edge aperture.
When the 3rd, freezing, the closed carrier of double flute of the present utility model can be under conditions of room temperature, and level is put into 0.25 milli
Rise in straw overcoat, heat-seal then is carried out to straw overcoat with heat-sealing instrument, reaches complete with liquid nitrogen or liquid nitrogen vapor in freezing
The enclosed effect of full physical isolation.
4th, the closed carrier total length of double flute of the present utility model only 80 millimeters, will than current commercialized carrier
It is short by more than 30%, on traditional freezing support, double-deck storage can be taken, helps to increase the storage quantity in liquid nitrogen container.
Brief description of the drawings
Accompanying drawing 1 is a kind of signal for being applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum
Figure.
Accompanying drawing 2 is a kind of carrier for being applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum
Panel schematic diagram.
Accompanying drawing 3 is a kind of double flute for being applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum
Loading part schematic diagram.
Accompanying drawing 4 is a kind of carrier for being applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum
The combination schematic diagram of panel and double flute loading part.
Accompanying drawing 5 is a kind of forward sight for being applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum
Figure.
Accompanying drawing 6 is a kind of signal for being applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum
Figure.
Embodiment
With reference to embodiment and the utility model is further described referring to the drawings.
The reference and part being related in accompanying drawing are as follows：
1st, carrier panel
11st, cylindrical hole
2nd, double flute loading part
21st, loading chute
3rd, operation handle
Embodiment 1
Accompanying drawing 1, accompanying drawing 2, accompanying drawing 3, accompanying drawing 4, accompanying drawing 5 are refer to, accompanying drawing 1 is that one kind of the present embodiment is applied to freezing people
The schematic diagram of the closed carrier of the double flute of class rareness sperm and embryo and ovum, accompanying drawing 2 are that one kind of the present embodiment is applied to
The carrier panel schematic diagram of mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum is freezed, accompanying drawing 3 is this implementation
A kind of double flute loading part signal for being applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum of example
Figure, accompanying drawing 4 are that a kind of of the present embodiment is applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum
Carrier panel and double flute loading part combination schematic diagram, accompanying drawing 5 is that one kind of the present embodiment is applied to the rare essence of the freezing mankind
The front view of son and the closed carrier of the double flute of embryo and ovum.
Described is applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum by carrier panel
1st, double flute loading part 2 and operation handle 3 form.
Described carrier panel 1 is the plate of both sides chamfering, centre provided with the cylindrical hole 11 run through, institute above and below a head
The afterbody for the carrier panel 1 stated connects with the head of double flute loading part 2.
The assembly that described double flute loading part 2 cuts into for cuboid, the described head of double flute loading part 2 left and right sides
An arcuate stream line body is respectively cut off, left and right sides surface respectively sets a loading chute 21.
The described afterbody of double flute loading part 2 and operation handle 3 connects, and described operation handle 3 is a cuboid.
A length of 3~6mm, a width of 2~2.3mm of described carrier panel 1.
Described 11 a diameter of 0~1.5mm of cylindrical hole, described cylindrical hole 11 apart from the edge 0.5 of carrier panel 1~
2mm。
Described 2 a length of 25~35mm, a width of 2~2.3mm, described two groove faces of loading chute 21 of double flute loading part are wide
1.2~1.6mm, 0.8~1mm of groove depth.
Described operation handle 3 grows 35~45mm, wide 2~2.3mm, high 2~2.3mm.
The closed carrier of described double flute also includes 0.25ml straw.
Embodiment 2
Accompanying drawing 2, accompanying drawing 3, accompanying drawing 4, accompanying drawing 5, accompanying drawing 6 are refer to, accompanying drawing 2 is that one kind of the present embodiment is applied to freezing people
The carrier panel schematic diagram of the closed carrier of the double flute of class rareness sperm and embryo and ovum, accompanying drawing 3 are the one of the present embodiment
Kind is applied to the double flute loading part schematic diagram of freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum, accompanying drawing
4 be a kind of carrier side for being applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum of the present embodiment
The combination schematic diagram of plate and double flute loading part, accompanying drawing 5 are that one kind of the present embodiment is applied to freezing mankind's rareness sperm and embryo
The front view of the closed carrier of the double flute of tire and ovum, accompanying drawing 6 are that one kind of the present embodiment is applied to freezing mankind's rareness sperm
And the schematic diagram of the closed carrier of the double flute of embryo and ovum.
Described is applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum by carrier panel
1st, double flute loading part 2 and operation handle 3 form.
Described carrier panel 1 is the plate of both sides chamfering, centre provided with the cylindrical hole 11 run through, institute above and below a head
The afterbody for the carrier panel 1 stated connects with the head of double flute loading part 2.
The assembly that described double flute loading part 2 cuts into for cylinder, the described head of double flute loading part 2 left and right sides
An arcuate stream line body is respectively cut off, left and right sides surface respectively sets a loading chute 21, the described head end opening of loading chute 21, tail
Portion's joint face tilts upward.
The described afterbody of double flute loading part 2 and operation handle 3 connects, and described operation handle 3 is a cylinder.
A length of 3~6mm, a width of 2~2.3mm of described carrier panel 1.
Described 11 a diameter of 0~1.5mm of cylindrical hole, described cylindrical hole 11 is apart from the leading edge 0.5 of carrier panel 1
~2mm.
Described 2 a length of 25~35mm, a width of 2~2.3mm, described two groove faces of loading chute 21 of double flute loading part are wide
1.2~1.6mm, 0.8~1mm of groove depth.
Described operation handle 3 grows 35~45mm, and basal diameter is 2.8~3.2mm.
The closed carrier of described double flute also includes 0.25ml straw.
Embodiment 3
Accompanying drawing 1, accompanying drawing 2, accompanying drawing 3, accompanying drawing 4, accompanying drawing 5 are refer to, accompanying drawing 1 is that one kind of the present embodiment is applied to freezing people
The schematic diagram of the closed carrier of the double flute of class rareness sperm and embryo and ovum, accompanying drawing 2 are that one kind of the present embodiment is applied to
The carrier panel schematic diagram of mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum is freezed, accompanying drawing 3 is this implementation
A kind of double flute loading part signal for being applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum of example
Figure, accompanying drawing 4 are that a kind of of the present embodiment is applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum
Carrier panel and double flute loading part combination schematic diagram, accompanying drawing 5 is that one kind of the present embodiment is applied to the rare essence of the freezing mankind
The front view of son and the closed carrier of the double flute of embryo and ovum.
Described is applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum by carrier panel
1st, double flute loading part 2 and operation handle 3 form.
The described freezing mankind's rareness sperm and the material of the closed carrier of the double flute of embryo and ovum of being applied to can be
Medical plastic (such as PVC, PETG) or medical resin, there is the characteristics of safe and non-toxic, low temperature resistant freezing.Used in practical operation
In can load for up to 20 microlitres of seminal fluid.The formation of ice crystal is most important freezing injury in seminal fluid freeze thawing, and is improved cold
Freeze and the speed of recovery is believed to effective formation for reducing ice crystal, semen sample can be made by double flute loading part 2 now
It is evenly distributed on by the surface tension of its liquid in two loading chutes 21 of double flute loading part 2, with connecing for liquid nitrogen and resuscitation fluid
Contacting surface product is bigger, and compared with traditional 2 milliliters of spermatozoa cryopreservation pipes (its built-in seminal fluid is piled into cylindric), seminal fluid can be with freeze thawing
Cooling and rewarming speed faster are obtained, so as to reduce freezing injury.Embryo or ovum can be loaded in cryoprotector
In cylindrical hole 11 above carrier panel 1.Rare sperm or ovum embryo is either freezed, freezing sample can after loading
So that under conditions of room temperature, level is put into 0.25 milliliter of straw overcoat, heat-seal then is carried out to straw overcoat with heat-sealing instrument,
Reach enclosed effect.
Most 20 microlitres of treated sperm sample is drawn when freezing rare sperm with pipettor first to be spread evenly across
In two loading chutes 21 of double flute loading part 2, under conditions of room temperature, carrier levels are put into 0.25 milliliter of straw overcoat, so
Heat-seal is carried out to straw overcoat with heat-sealing instrument afterwards, reaches enclosed effect.By on the carrier levels dislocation liquid nitrogen of heat-seal
(- 120 DEG C) place of 4 centimetres of side 5 minutes, it is placed in liquid nitrogen and preserves after sample turns into solid-state.
Thawed rare sperm before monosperm ooecium slurry injection (ICSI) is carried out, and closed carrier is removed in liquid nitrogen,
0.25 milliliter of straw overcoat is cut off with scissors, carrier is taken out and is directly placed into the Falcon covered with mineral oil through 37 DEG C of preheatings
In 10 microlitres of culture mediums in 1006 wares (i.e. used in ICSI), then direct row ICSI；Or take out carrier and be directly placed into through 37
In the Eppendorf centrifuge tubes equipped with 1 milliliter of culture medium of DEG C preheating, then slightly centrifuge, wash away partial freeze protective agent, then will
Remaining sperm adds row ICSI after the wares of Falcon 1006.
In addition this carrier total length only 80 millimeters, will be short by more than 30% than current commercialized carrier, in tradition
Freezing support on, double-deck storage can be taken, help to increase storage quantity in liquid nitrogen container, the freezing of embryo or ovum
Method is identical with each commercialization freezing Reagent Company.
Described above is only preferred embodiment of the present utility model, it is noted that for the common skill of the art
Art personnel, on the premise of the utility model principle is not departed from, some improvement and supplement can also be made, these are improved and supplement
Also it should be regarded as the scope of protection of the utility model.
Claims (2)
1. a kind of be applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum, it is characterised in that institute
The closed carrier of double flute stated is made up of carrier panel, double flute loading part and operation handle；Described carrier panel is a head
Portion both sides chamferings, the middle plate for being provided with the cylindrical hole run through up and down, the afterbody of described carrier panel and double flute loading part
Head connects；The assembly that described double flute loading part cuts into for cuboid, the described double flute loading part head left and right sides
An arcuate stream line body is respectively cut off, left and right sides surface respectively sets a loading chute；Described double flute loading part afterbody and operation
Handle connects, and described operation handle is a cuboid；3~6mm of length, the wide 2~2.3mm of described carrier panel, it is described
The a diameter of 0~1.5mm of cylindrical hole, described cylindrical hole is apart from 0.5~2mm of carrier face plate edge, described double flute dress
25~35mm, wide 2~2.3mm, described two wide 1.2~1.6mm of loading chute groove face of minister, groove depth 0.8~1mm are carried, it is described
Operation handle length 35~45mm, wide 2~2.3mm, high 2~2.3mm.
2. the closed carrier of double flute according to claim 1, it is characterised in that the closed carrier of described double flute also includes
0.25ml straw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720765038.7U CN206949363U (en) | 2017-06-28 | 2017-06-28 | It is a kind of to be applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720765038.7U CN206949363U (en) | 2017-06-28 | 2017-06-28 | It is a kind of to be applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum |
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| Publication Number | Publication Date |
|---|---|
| CN206949363U true CN206949363U (en) | 2018-02-02 |
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| CN201720765038.7U Active CN206949363U (en) | 2017-06-28 | 2017-06-28 | It is a kind of to be applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114027295A (en) * | 2021-12-16 | 2022-02-11 | 草原和牛投资有限公司 | Oocyte and embryo vitrification freezing carrier and method |
| WO2022184051A1 (en) * | 2021-03-03 | 2022-09-09 | 深圳拜尔洛克生物技术有限公司 | Carrier for cryopreservation or thawing and resuscitation of biological tissue |
-
2017
- 2017-06-28 CN CN201720765038.7U patent/CN206949363U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022184051A1 (en) * | 2021-03-03 | 2022-09-09 | 深圳拜尔洛克生物技术有限公司 | Carrier for cryopreservation or thawing and resuscitation of biological tissue |
| CN114027295A (en) * | 2021-12-16 | 2022-02-11 | 草原和牛投资有限公司 | Oocyte and embryo vitrification freezing carrier and method |
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