CN203653553U - Real-time sampling in-vitro culture fermentation bottle - Google Patents
Real-time sampling in-vitro culture fermentation bottle Download PDFInfo
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- CN203653553U CN203653553U CN201420024429.XU CN201420024429U CN203653553U CN 203653553 U CN203653553 U CN 203653553U CN 201420024429 U CN201420024429 U CN 201420024429U CN 203653553 U CN203653553 U CN 203653553U
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Abstract
The utility model relates to a real-time sampling in-vitro culture fermentation bottle which comprises a bottle body and a bottle cover, wherein the bottle cover is in threaded connection to the top bottleneck of the bottle body. The bottle further comprises a three-way valve, an electronic counter and an electromagnetic induction elastic piece, wherein the three-way valve has an inlet, an outlet I and an outlet II. The electronic counter is arranged on the bottle cover, the electromagnetic induction elastic piece is connected to the outlet I of the three-way valve, and the electromagnetic induction elastic piece is connected with the electronic counter through a connecting line; the bottle cover is provided with an exhaust hole and a liquid sampling hole. The exhaust hole is communicated with the inlet of the three-way valve. The liquid sampling hole is provided with a switch and is connected with a sampling pipe. A lower pipe orifice of the sampling pipe is arranged at the bottom of the bottle body. According to the bottle, in the whole culturing and sampling process, the cover of the culture bottle needs not to be opened to realize statistics of gas generation rate and sampling of gas and liquid samples, so that the bottle brings convenience to scientific researches.
Description
Technical field
The utility model relates to the research fields such as food, fermentation, microorganism and medical science, relates in particular to the improvement to real-time sampling vitro culture fermentation flask.
Background technology
Vitro culture fermentation technique is one of experimental technique comparatively conventional in scientific effort, regularly collects nutrient solution, carries out the sample determination of different time points, is the important means of research trends fermentation or microorganism dynamic change.And traditional vitro culture fermentation flask needs to open culturing bottle in the process of sampling, this has caused the change of culture condition to a certain extent, and then the measurement result in later stage is exerted an influence.Therefore develop real-time sampling vitro culture fermentation flask and there is important scientific research value and application potential.
Under condition of in vitro culture, conventional culturing bottle exist resistance to air loss tight, sample loaded down with trivial details, sample length consuming time and the larger drawback of measurement result impact on the later stage, the gimmick of sampling also can produce larger variation because opening cultivation bottle cap in addition, and above these all can produce larger impact to the measurement result of terminal.Design novel culturing bottle, can control sampling depth, sampling position, reduce the impact of the later stage being cultivated because of sampling as small as possible, by for producing, test gimmick that scientific research provides standard more and test-results more accurately.
Utility model content
The utility model, for above problem, provides a kind of simple in structure, has ensured sampling reliability and the real-time sampling vitro culture fermentation flask of being convenient to gas production, mensuration gas production rate.
The technical solution of the utility model is: comprise bottle and bottle cap, described bottle cap is threaded connection on the bottleneck of the top of described bottle; Also comprise T-valve, electronic counter and electromagnetic induction shell fragment, described T-valve has import, outlet one and outlet two, described electronic counter is located on described bottle cap, described electromagnetic induction shell fragment is connected in the outlet one of described T-valve, and described electromagnetic induction shell fragment connects described electronic counter by line;
Described bottle cap is provided with venting hole and liquid sample hole, and described venting hole is communicated with the import of described T-valve;
Described liquid sample hole is provided with switch, and described liquid sample hole is connected with sampling tube, and the lower nozzle of described sampling tube is located at the bottom of described bottle.
In described venting hole, be provided with filter screen.
In described bottle cap, be provided with silicone gasket.
Also comprise bottlecap cover, described bottlecap cover is connected to the top of described bottle cap, and described bottlecap cover is provided with pore.
The utility model has improved traditional common rubber plug type culturing bottle, designed there is accurate thread, venting hole, liquid sample hole, gas sampling hole, real time record gas production rate, preferably culturing bottle of stopping property.From stopping property aspect, designed glass bottle opening and the corresponding pp material bottle cap of screw thread, tighten bottle cap and can keep sealing, in addition at bottle cap liner with silicone gasket, guaranteed the resistance to air loss of bottleneck in culturing process.
Above venting hole, T-valve, electronic counter and electromagnetic induction shell fragment are installed, can be according to the automatic opening switch of pressure, get rid of unnecessary gas, in good time decompression, the disconnection of electromagnetic induction shell fragment, engages once, can realize the record increase of counter once.When use, in advance electromagnetic induction shell fragment is opened once to needed gas volume and proofread and correct, in the process of cultivating, can, according to the numerical value above counter, calculate the volume of aerogenesis, thereby realize the mensuration of gas production rate.Simultaneously, can carry out as required the collection of gaseous sample, when gaseous sample gathers, syringe is inserted in T-valve, can realize opening of sampling path by adjusting the switch of T-valve, after extracting gases sample, first close T-valve, after extract sampling injector, whole gas sampling process is less on the impact of the interior environment that ferments.
On bottle cap, offer liquid sample hole, liquid sample hole is connected with a plastic conduit, is directly passed into culturing bottle bottom.Liquid sample hole and connecting valve above bottle cap, when fermentation culture, close switch, when collection liquid sample, opens switch syringe and extract, and after sampling finishes, closing switch, continues the cultivation in later stage.Adopt hole sampling by liquid, avoided traditional king-size sampling needle sampling drawback of utilizing, convenient and accurate, less on the impact that the later stage cultivates.
In whole cultivation, sampling process, needn't open the lid of culturing bottle, can realize gas yield statistics, the sampling of gas and liquid sample, and culturing bottle remains in position, has guaranteed scientific effort to be offered convenience the consistence of cultivating.
Brief description of the drawings
Fig. 1 is disassemblying structure schematic diagram of the present utility model;
In figure, 1 is bottle, the 2nd, and bottle cap, the 3rd, T-valve, the 4th, electronic counter, the 5th, electromagnetic induction shell fragment, the 6th, venting hole, the 7th, liquid sample hole, the 8th, sampling tube, the 9th, filter screen, the 10th, silicone gasket, the 11st, bottlecap cover, 12 vapor pipes.
Embodiment
The utility model as shown in Figure 1, comprises bottle 1 and bottle cap 2, and described bottle cap 2 is threaded connection on the bottleneck of the top of described bottle 1; Also comprise T-valve 3, electronic counter 4 and electromagnetic induction shell fragment 5, described T-valve 3 has import, outlet one and outlet two, described electronic counter 4 is located on described bottle cap 2 (being connected by screw), described electromagnetic induction shell fragment 5 is connected to the outlet one (for exhaust) of described T-valve, and described electromagnetic induction shell fragment 5 connects described electronic counter 4 by line; In application, outlet one connects vapor pipe 12, and electromagnetic induction shell fragment 5 is located in vapor pipe 12, when gaseous tension is large, pushes electromagnetic induction shell fragment 5 open, pressure hour, and electromagnetic induction shell fragment 5 covers vapor pipe; Outlet two is for gathering gas.
Described bottle cap 2 is provided with venting hole 6 and liquid sample hole 7, and described venting hole 6 is communicated with the import of described T-valve 3;
Described liquid sample hole 7 is provided with switch, and described liquid sample hole 7 is connected with sampling tube 8, and the lower nozzle of described sampling tube 8 is located at the bottom of described bottle 1, has avoided in gas from liquid sampling tube, overflowing in Process of in vitro.
In described venting hole 6, be provided with filter screen 9, avoid venting hole 6 blockage phenomenon, ensure the reliability of exhaust.
In described bottle cap 2, be provided with silicone gasket 10, ensure stopping property.
Also comprise bottlecap cover 11, described bottlecap cover 11 is connected to the top of described bottle cap 2, and described bottlecap cover 11 is provided with pore.Bottlecap cover is transparent color and is connected with bottle cap, and the pore in bottlecap cover, guarantees the discharge of unnecessary gas, is conducive to protect electronic counter simultaneously.
The utility model, according to the requirement in the process of vitro culture and when sampling, designs the bottle cap part of cellar culture bottle.Guarantee, under the prerequisite of stopping property, to carry out the design in voltage stabilizing and real-time sampling hole, In Vitro Fermentation is cultivated to gas production rate simultaneously and measure.The real-time sampling vitro culture fermentation flask of design is made up of screw thread mouth culturing bottle, sealing plain washer, screw thread mouth bottle cap, vapor pipe, electromagnetic induction shell fragment, electronic counter, thieff hatch etc., greatly facilitate the progress in sampling process, stopping property, the sealing anaerobism of whole culturing process, the accuracy of measurement result are improved, due to the use of electronic counter, saved the labor capacity in conventional vitro culture fermenting process simultaneously.
Utilize real-time sampling vitro culture fermentation flask to cultivate sampling, there is significant convenience.Compared with conventional rubber plug type culturing bottle, by thieff hatch collected specimens each about 10 seconds consuming time, can singlely complete, and do not change the anaerobically fermenting environment in culturing bottle.And traditional culturing bottle needs two people, the sampling of uncapping, easily changes yeasting, and those skilled in the art need 50-65 to complete second, time-consuming longer, and real-time sampling vitro culture fermentation flask has shortened the sampling time.Traditional open plug sampling, changed the nutrient solution pH after sampling, and nutrient solution pH in real-time sampling culturing bottle is more stable.By counter records gas production rate, it is the function that the outer cultivation and fermentation bottle of conventional bulk does not have.And by electromagnetic recording, guarantee the accuracy of record, saved a large amount of manpowers, especially needed 24 hours even when the cultured continuously of longer time.In whole culturing process, the result stability of utilizing real-time sampling culturing bottle to obtain is better, and effect is more consistent, has reached effect fast and accurately.Due to easy and simple to handle, omit some loaded down with trivial details links, improve the efficiency of the worker management of unit culturing bottle.
Claims (4)
1. real-time sampling vitro culture fermentation flask, comprises bottle and bottle cap, and described bottle cap is threaded connection on the bottleneck of the top of described bottle; It is characterized in that, also comprise T-valve, electronic counter and electromagnetic induction shell fragment, described T-valve has import, outlet one and outlet two, described electronic counter is located on described bottle cap, described electromagnetic induction shell fragment is connected in the outlet one of described T-valve, and described electromagnetic induction shell fragment connects described electronic counter by line;
Described bottle cap is provided with venting hole and liquid sample hole, and described venting hole is communicated with the import of described T-valve;
Described liquid sample hole is provided with switch, and described liquid sample hole is connected with sampling tube, and the lower nozzle of described sampling tube is located at the bottom of described bottle.
2. real-time sampling vitro culture fermentation flask according to claim 1, is characterized in that, in described venting hole, is provided with filter screen.
3. real-time sampling vitro culture fermentation flask according to claim 1, is characterized in that, in described bottle cap, is provided with silicone gasket.
4. real-time sampling vitro culture fermentation flask according to claim 1, is characterized in that, also comprise bottlecap cover, described bottlecap cover is connected to the top of described bottle cap, and described bottlecap cover is provided with pore.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420024429.XU CN203653553U (en) | 2014-01-15 | 2014-01-15 | Real-time sampling in-vitro culture fermentation bottle |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420024429.XU CN203653553U (en) | 2014-01-15 | 2014-01-15 | Real-time sampling in-vitro culture fermentation bottle |
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| Publication Number | Publication Date |
|---|---|
| CN203653553U true CN203653553U (en) | 2014-06-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420024429.XU Expired - Fee Related CN203653553U (en) | 2014-01-15 | 2014-01-15 | Real-time sampling in-vitro culture fermentation bottle |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434323A (en) * | 2016-12-05 | 2017-02-22 | 无锡艾科瑞思产品设计与研究有限公司 | Microorganism culture and detection device capable of controlling oxygen environments |
| CN109401960A (en) * | 2017-08-16 | 2019-03-01 | 苏州米迪生物技术有限公司 | The high efficiency cell culture bottle of built-in disturbing flow device |
| CN113899865A (en) * | 2021-12-09 | 2022-01-07 | 中国农业科学院农业环境与可持续发展研究所 | Carbon-nitrogen conversion measurement system based on Internet of things |
-
2014
- 2014-01-15 CN CN201420024429.XU patent/CN203653553U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434323A (en) * | 2016-12-05 | 2017-02-22 | 无锡艾科瑞思产品设计与研究有限公司 | Microorganism culture and detection device capable of controlling oxygen environments |
| CN109401960A (en) * | 2017-08-16 | 2019-03-01 | 苏州米迪生物技术有限公司 | The high efficiency cell culture bottle of built-in disturbing flow device |
| CN113899865A (en) * | 2021-12-09 | 2022-01-07 | 中国农业科学院农业环境与可持续发展研究所 | Carbon-nitrogen conversion measurement system based on Internet of things |
| CN113899865B (en) * | 2021-12-09 | 2022-04-08 | 中国农业科学院农业环境与可持续发展研究所 | Carbon-nitrogen conversion measurement system based on Internet of things |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140618 Termination date: 20150115 |
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| EXPY | Termination of patent right or utility model |