CN206420774U - A kind of air microbe device for fast detecting - Google Patents
A kind of air microbe device for fast detecting Download PDFInfo
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- CN206420774U CN206420774U CN201720050664.8U CN201720050664U CN206420774U CN 206420774 U CN206420774 U CN 206420774U CN 201720050664 U CN201720050664 U CN 201720050664U CN 206420774 U CN206420774 U CN 206420774U
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- 239000000523 sample Substances 0.000 claims abstract description 20
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- 239000007850 fluorescent dye Substances 0.000 claims abstract description 11
- 238000001514 detection method Methods 0.000 abstract description 24
- 238000012544 monitoring process Methods 0.000 abstract description 6
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 230000003321 amplification Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
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- 238000005286 illumination Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 230000010365 information processing Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000013618 particulate matter Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000638935 Senecio crassissimus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000010276 collecting duct carcinoma Diseases 0.000 description 1
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- 238000013461 design Methods 0.000 description 1
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- 230000000694 effects Effects 0.000 description 1
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- 230000001954 sterilising effect Effects 0.000 description 1
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Abstract
A kind of air microbe device for fast detecting, belongs to environmental monitoring technology field.LASER Light Source, convergent lens, pinhole plate, collimation lens, diaphragm, fourier transform lenses, sample cell and central detector are on same central axis, are from left to right arranged successively；Central detector is located on the focal plane on the right side of fourier transform lenses, and central detector is rearranged by semi-circular shape photodetection face, and the annular width in semi-circular shape photodetection face is outwards gradually increased by justifying center, and center is hollow out；Right angle detector is located on central axis upright direction；Annular detector is located on the circular arc that central detector is constituted with right angle detector, and annular detector is made up of a series of rectangle photodetection face of arc shootings at equal intervals；Right angle detector, annular detector, central detector, fluorescent probe are connected by cable with data processing unit；The device avoids complex operations processes such as the amplification of traditional detection method, culture and marks, with real-time, efficient advantage.
Description
Technical field
The utility model belongs to environmental monitoring technology field, and in particular to a kind of air microbe device for fast detecting.
Background technology
Air microbe detection technique environmental monitoring, the monitoring of pharmaceutical industry environmental standard etc. in anti-terrorism safety, building
Field has wide application.But traditional air microbe detection technique, such as solid impact formula air microbe detection method
(Lu Yanying, Wang Jin, Zhu Rong, what emerging flourish indoor public places air microbe detection and method for disinfection and sterilization, 2008 years whole nations
The academic meeting of wind and atmospheric environment, on October 21st, 2008, Beijing, pp.272-276), the detection of natural subsidence formula air microbe
Method (Chen E, Wan Dong, Chu Kecheng, Xu Shuqing, the monitoring of a peaceful air microbial contamination and progress, development fields of environmental monitoring in china,
Volume 2014,30, the 4th phase, pp.171-178) and UF membrane detection method (Xu Dongming, tension and relaxation, Zhang Xiquan, Zhao Hong rosy clouds microfiltration membranes
Application of the isolation technics in terms of CDCs microorganism detection, Chinese countryside health, the 2015, the 12nd phase, pp.41-42) etc.,
It is generally necessary to which the complex operations process such as sample collection, culture, amplification, mark, required time is long, it is difficult to realize quick inspection in real time
Survey.Therefore, quick air microbe detection means is researched and developed to be of great significance and value.
Utility model content
Sampling, culture, amplification, the mark that the utility model exists for conventional air microbial detection device etc. is time-consuming to be taken
Power, it is difficult to the problem of realizing quick detection in real time, proposes a kind of quick air microbe detection means.
The technical solution of the utility model：
A kind of air microbe device for fast detecting, including LASER Light Source, convergent lens, pinhole plate, collimation lens, light
Door screen, fourier transform lenses, sample cell, right angle detector, annular detector, central detector, fluorescent probe, signal processing unit
And air pump；At LASER Light Source, convergent lens, pinhole plate, collimation lens, diaphragm, fourier transform lenses, sample cell and central detector
On same central axis, from left to right arrange successively；Central detector is located on the focal plane on the right side of fourier transform lenses, center
Detector is rearranged by semi-circular shape photodetection face, the annular width in semi-circular shape photodetection face by justify center outwards by
Cumulative to add, center is hollow out；Right angle detector is located on central axis upright direction；Annular detector be located at central detector with
On the circular arc that right angle detector is constituted, annular detector is made up of a series of rectangle photodetection face of arc shootings at equal intervals；
Right angle detector, annular detector, central detector, fluorescent probe are connected by cable with data processing unit；
Detected that step is as follows with above-mentioned air microbe device for fast detecting：
A. the laser of LASER Light Source output is converged on focal plane by convergent lens, and focal plane places pinhole plate, warp
Pinhole plate incides collimation lens after filtering, and is expanded by collimation lens and incides diaphragm for directional light, the light exported through diaphragm by
Fourier transform lenses focusing illumination is on sample cell；
B. air sample to be measured is pumped into sample cell, sky of the laser through fourier transform lenses focusing illumination into sample cell by air pump
Gas sample product；Particulate matter in air causes the scattering of light, and scattered light is by right angle detector, annular detector and central detector
Detection；Microorganism in air is excited light irradiation transmitting fluorescence, and fluorescence is detected by fluorescent probe；
C. scattering optical information, the fluorescent probe that right angle detector, annular detector and central detector detection are obtained are visited
The fluorescence information measured is respectively through cable transmission to signal processing unit；Signal processing unit is carried out according to optical scattering principle
Optical information processing and analysis are scattered, the size and number information of particulate in air is obtained；Signal processing unit shines according to laser
The wavelength of fluorescence for penetrating microorganism generation analyzes the type and quantity for obtaining microorganism with strength information；Finally realize the micro- life of air
Species, the quick detection of size and number information of thing.
The beneficial effects of the utility model：
(1) the utility model greatly reduces detection time without operations such as amplification, culture and marks, realizes to air
The quick detection of sample.
(2) the utility model is detected the size sum for obtaining particulate in air by signal processing unit using optical scattering
Amount, the type and quantity of microorganism is obtained using fluorescence detection, it is to avoid the artificial observation of traditional detection method, with real-time height
The advantage of effect.
Brief description of the drawings
Fig. 1 is the utility model air microbe device for fast detecting figure.
Fig. 2 is the utility model air microbe device for fast detecting top view.
Fig. 3 is the utility model central detector structure chart.
In figure：1 LASER Light Source；2 convergent lenses；3 pinhole plates；4 collimation lenses；5 diaphragms；
6 fourier transform lenses；7 sample cells；81 right angle detectors；82 annular detectors；83 central detectors；
9 fluorescent probes；10 data processing units；11 air pumps.
Embodiment
Below in conjunction with accompanying drawing and technical scheme, embodiment of the present utility model is further illustrated.
The utility model air microbe device for fast detecting detects the chi for obtaining particulate in air using optical scattering
Very little and quantity, the type and quantity of microorganism are obtained using fluorescence detection, the quick detection of microbes in air is realized.
Embodiment
Fig. 1 is the utility model air microbe device for fast detecting schematic diagram.The device includes LASER Light Source, assembles saturating
It is mirror, pinhole plate, collimation lens, diaphragm, fourier transform lenses, sample cell, right angle detector, annular detector, central detector, glimmering
Photo-detector, signal processing unit, air pump.
Fig. 2 is the utility model air microbe device for fast detecting top view, described LASER Light Source, convergent lens,
Pinhole plate, collimation lens, diaphragm, fourier transform lenses, sample cell, central detector are on same central axis.
The central detector is located on the focal plane on the right side of the fourier transform lenses of central axis.As shown in figure 3, center is visited
Survey device to be rearranged by semi-circular shape photodetection face, the annular width in semi-circular shape photodetection face is by justifying center outwards gradually
Increase, circle center is hollow out.
The right angle detector is located on central axis upright direction.
The annular detector is located on the circular arc that central detector is constituted with right angle detector, and annular detector is by one
Arrange the rectangle photodetection face composition of arc shooting at equal intervals.
The scattering photo detecting unit, fluorescent probe are connected by cable with data processing unit.
Air microbe device for fast detecting follows the steps below detection：
The laser of LASER Light Source output is converged on focal plane by convergent lens, and focal plane places pinhole plate, through pin
Orifice plate incides collimation lens after filtering, and is expanded by collimation lens and incides diaphragm for directional light, the light exported through diaphragm is by Fu
Vertical leaf lens focus is irradiated on sample cell.
Air sample is pumped into sample cell, air sample of the laser through fourier transform lenses focusing illumination into sample cell by air pump
Product.Particulate matter in air causes the scattering of light, and scattered light is by right angle detector, the annular detection in scattering photo detecting unit
Device and central detector detection.Microorganism in air is excited light irradiation transmitting fluorescence, and fluorescence is detected by fluorescent probe.
Scattering optical information, the fluorescent probe that right angle detector, annular detector and central detector detection are obtained are detected
Obtained fluorescence information is respectively through cable transmission to signal processing unit.Signal processing unit is dissipated according to optical scattering principle
Optical information processing and analysis are penetrated, the size and number information of particulate in air is obtained.Signal processing unit irradiates according to laser
The wavelength of fluorescence that microorganism produces analyzes the type and quantity for obtaining microorganism with strength information.Finally realize air microbe
Species, the quick detection of size and number information.
It is described above, only the utility model preferably embodiment, but protection domain of the present utility model is not
It is confined to this.Any one skilled in the art is in the technical scope that the utility model is illustrated, according to this practicality
New technical scheme and its utility model design are subject to replace or change on an equal basis, should all cover in protection model of the present utility model
The row enclosed.
Claims (1)
1. a kind of air microbe device for fast detecting, it is characterised in that the air microbe device for fast detecting includes laser
It is light source (1), convergent lens (2), pinhole plate (3), collimation lens (4), diaphragm (5), fourier transform lenses (6), sample cell (7), straight
Angle detector (81), annular detector (82), central detector (83), fluorescent probe (9), signal processing unit (10) are gentle
Pump (11)；LASER Light Source (1), convergent lens (2), pinhole plate (3), collimation lens (4), diaphragm (5), fourier transform lenses (6), sample
Product pond (7) and central detector (83) are on same central axis, are from left to right arranged successively；Central detector (83) is located at
On focal plane on the right side of fourier transform lenses (6), central detector (83) is rearranged by semi-circular shape photodetection face, semicircular ring
The annular width in shape photodetection face is outwards gradually increased by justifying center, and center is hollow out；Right angle detector (81) is located at center
In axis vertical direction；Annular detector (82) is located on the circular arc that central detector (83) is constituted with right angle detector (81),
Annular detector (82) is made up of a series of rectangle photodetection face of arc shootings at equal intervals；Right angle detector (81), annular
Detector (82), central detector (83), fluorescent probe (9) are connected by cable with data processing unit (10).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720050664.8U CN206420774U (en) | 2017-01-19 | 2017-01-19 | A kind of air microbe device for fast detecting |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720050664.8U CN206420774U (en) | 2017-01-19 | 2017-01-19 | A kind of air microbe device for fast detecting |
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| CN206420774U true CN206420774U (en) | 2017-08-18 |
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| CN201720050664.8U Expired - Fee Related CN206420774U (en) | 2017-01-19 | 2017-01-19 | A kind of air microbe device for fast detecting |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106596498A (en) * | 2017-01-19 | 2017-04-26 | 大连理工大学 | Device for quickly detecting air microorganisms |
| WO2019156632A1 (en) * | 2018-02-08 | 2019-08-15 | Agency For Science, Technology And Research | Particulate matter sensor module using compound parabolic/elliptical collector |
-
2017
- 2017-01-19 CN CN201720050664.8U patent/CN206420774U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106596498A (en) * | 2017-01-19 | 2017-04-26 | 大连理工大学 | Device for quickly detecting air microorganisms |
| CN106596498B (en) * | 2017-01-19 | 2018-09-04 | 大连理工大学 | A kind of air microbe device for fast detecting |
| WO2019156632A1 (en) * | 2018-02-08 | 2019-08-15 | Agency For Science, Technology And Research | Particulate matter sensor module using compound parabolic/elliptical collector |
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