CN201917509U - Flow cytometry - Google Patents
Flow cytometry Download PDFInfo
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- CN201917509U CN201917509U CN 201020613651 CN201020613651U CN201917509U CN 201917509 U CN201917509 U CN 201917509U CN 201020613651 CN201020613651 CN 201020613651 CN 201020613651 U CN201020613651 U CN 201020613651U CN 201917509 U CN201917509 U CN 201917509U
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Abstract
The utility model discloses a flow cytometry which comprises a light source illumination system, a liquid flow system, a light splitting system, a signal detection-analysis processing system, and a PC (personal computer) display control system. The liquid flow system comprises a flowing chamber; cells flowing linearly are arranged in the flowing chamber; a solid laser is used as an excitation light source of the light source illumination system; lasers are converged and irradiated on the flowing chamber through a reflector and a plane convex lens, and are further converged through an optical part integrated on the flowing chamber, and irradiated on the cells flowing linearly; the signal detection-analysis processing system comprises a lateral scattering detector, a yellow fluorescence channel detector, a near-infrared fluorescence channel detector, a forward scattering detector, and a signal processing module; and the detectors are all multi-pixel photon counters (MPPC). The flow cytometry has the characteristics of larger dynamic detection scope, high sensitivity, small and compact size, and convenience in maintenance.
Description
Technical field
The utility model belongs to a kind of biomedical detecting instrument, is specifically related to a kind of flow cytometer.
Background technology
(flow cytometer is that multi-door subjects such as light harvesting, electronics, fluid mechanics, cytochemistry, biology, immunology and laser and computing machine and technology are in the advanced science and technology equipment of one FCM) to flow cytometer.It is widely used in fields such as clinical medicine, cytology, biology, microbiology, pharmaceutics, genesiology, is one of advanced instrument in the modern scientific research, is described as breadboard " CT ".It to single-row cell in the quick streamlined flow state or biologic grain carry out one by one, the technology of multiparameter, qualitative, quantitative test fast or sorting, have characteristics such as detection speed is fast, measurement parameter is many, the image data amount big, analyze comprehensively, separating purity is high, method is flexible.
At present commercial flow cytometer adopts gas laser, solid state laser etc., and it is big to have a volume, complex structure, and the shortcoming that maintenance cost is high, some flow cytometer adopts single excitation source, has limited the selection of cell fluorescence dyestuff.The detector of flow cytometer generally adopts photomultiplier (PMT), and it is long-pending that it has large photosensistive surface, the advantage of low noise equivalent power (NEP), but bulky, quantum efficiency is low, and reverse biased is too high, only can be operated in UV and the limit of visible spectrum, to external magnetic field resistibility weakness.
The liquid control system of commercial flow cytometer costs an arm and a leg, and is bulky, complex structure.
The utility model content
For overcoming deficiency of the prior art, the purpose of this utility model is to provide a kind of bigger dynamic detecting range that has, high sensitivity, and volume is little and compact, is convenient to safeguard, really realizes portable flow cytometer.
In order to solve the problems of the technologies described above, realize above-mentioned purpose, the utility model is achieved through the following technical solutions:
A kind of flow cytometer, comprise source illumination system, liquid fluid system, beam splitting system, acquisition of signal analysis process system and PC display control program, described liquid fluid system makes cell sample make streamlined flow under the parcel of sheath stream, described source illumination system carries out laser radiation to the interested stream of cells of fluorochrome label in the described liquid fluid system, excite transmitting of the fluorescence that obtains institute's mark, enter described acquisition of signal analysis process system by described beam splitting system and carry out acquisition of signal, analyze and output, described PC display control program shows the information of described acquisition of signal analysis process system and stores and the described source illumination system of FEEDBACK CONTROL, liquid fluid system and acquisition of signal analysis process system, described liquid fluid system comprises flow chamber, the cell that has streamlined flow in the described flow chamber, one solid state laser is as the excitation source of source illumination system, laser is through catoptron, plano-convex lens is assembled and is shone on the flow chamber, further assemble through the opticator that is integrated in flow chamber, make laser radiation on the cell of streamlined flow.
Further, described acquisition of signal analysis process system comprises a lateral scattering detector, a yellow fluorescence channel detector and a near-infrared fluorescent channel detector that is separately positioned on after the described beam splitting system, also comprise a forward scattering detector, and the signal processing module of the described forward scattering detector of a connection, lateral scattering detector, yellow fluorescence channel detector and near-infrared fluorescent channel detector.
Preferably, described lateral scattering detector, yellow fluorescence channel detector and near-infrared fluorescent channel detector are formed detector module, and described flow chamber, beam splitting system and the detector module drawn together formed a point-to-point imaging system.
Further, also be provided with a light barrier between described forward scattering detector and the described liquid fluid system.
Further, described forward scattering detector, lateral scattering detector, yellow fluorescence channel detector and near-infrared fluorescent channel detector are all many pixels photon counter (MPPC).
By the utilization of above technology, the utlity model has following advantage:
1, adopts many pixels photon counter (MPPC), the array that it has a plurality of avalanche diodes (APD) to form, constituted the output of MPPC from the summation of the pixel of each APD output, has good photon counting performance, low bias voltage, fabulous temporal resolution, high-gain, high sensitivity, the advantage that volume is little can make instrument little and compact, highly sensitive.
2, adopt point-to-point imaging system, can make the scattered light of flow chamber cell and the faint fluorescence that produces that is excited better be detected the device response.
Above-mentioned explanation only is the general introduction of technical solutions of the utility model, for can clearer understanding technological means of the present utility model, and can be implemented according to the content of instructions, below with preferred embodiment of the present utility model and conjunction with figs. describe in detail as after.Embodiment of the present utility model is provided in detail by following examples and accompanying drawing thereof.
Description of drawings
Below in conjunction with drawings and embodiments the utility model is described in further detail.
Fig. 1 is the structural representation of an embodiment of flow cytometer of the present utility model.
Fig. 2 is the point-to-point imaging system structural representation of an embodiment of flow cytometer of the present utility model.
Number in the figure explanation: 1, solid state laser, 2, catoptron, 3, plano-convex lens, 4, flow chamber, 5, the cell of streamlined flow, 6, light barrier, 7, forward scattering detector, 8, lateral scattering detector, 9, the yellow fluorescence channel detector, 10, the near-infrared fluorescent channel detector, 11, beam splitting system, 12, signal processing analysis module, 13, the PC display control program, 14, signal processing system, 15, source illumination system, 16, liquid fluid system, 17, point-to-point imaging system, 18, detector module.
Embodiment
Referring to shown in Figure 1, a kind of flow cytometer, comprise source illumination system 15, liquid fluid system 16, beam splitting system 11, acquisition of signal analysis process system 14 and PC display control program 13, described liquid fluid system 16 makes cell sample make streamlined flow under the parcel of sheath stream, the interested stream of cells of fluorochrome label is carried out laser radiation in 15 pairs of described liquid fluid systems 16 of described source illumination system, excite transmitting of the fluorescence that obtains institute's mark, enter described acquisition of signal analysis process system 14 by described beam splitting system 11 and carry out acquisition of signal, analyze and output, the information of 13 pairs of described acquisition of signal analysis process systems 14 of described PC display control program shows and stores and the described source illumination system 15 of FEEDBACK CONTROL, liquid fluid system 16 and acquisition of signal analysis process system 14, described liquid fluid system 16 comprises flow chamber 4, has the cell 5 of streamlined flow in the described flow chamber 4.
It is excitation source that the flow cytometer of present embodiment adopts the solid state laser 1 of 532nm, laser is assembled and is shone on the flow chamber 4 through catoptron 2, plano-convex lens 3, further assemble through the opticator that is integrated in flow chamber, make laser radiation on the cell 5 of streamlined flow.
Described acquisition of signal analysis process system comprises a lateral scattering detector 8, a yellow fluorescence channel detector 9 and a near-infrared fluorescent channel detector 10 that is separately positioned on after the described beam splitting system 11, also comprise a forward scattering detector 7, and the signal processing module 12 of the described forward scattering detector 7 of a connection, lateral scattering detector 8, yellow fluorescence channel detector 9 and near-infrared fluorescent channel detector 10.
Further, described forward scattering detector 7, lateral scattering detector 8, yellow fluorescence channel detector 9 and near-infrared fluorescent channel detector 10 are all many pixels photon counter.
Further, forward scattering light is more intense, makes forward scattering detector 7 saturated easily, allows a small amount of forward scattering light enter forward scattering detector 7 to survey so be aided with little light barrier 6.
Further, side scattered light, the fluorescence that excites generation are assembled through the lens in the beam splitting system 11, mating plate makes scattered light after filtration then, the fluorescence approaching side is to scattering detector 8, yellow fluorescence channel detector 9 and near-infrared fluorescent channel detector 10, converge on the detector through lens, wherein lateral scattering detector 8 is that detection channels, the yellow fluorescence channel detector 9 of scattered light 532nm wavelength is the detection channels of near infrared 780nm wavelength for the detection channels of yellow fluorescence 585nm wavelength, near-infrared fluorescent channel detector 10 again.
Preferably, referring to shown in Figure 2, described lateral scattering detector 8, yellow fluorescence channel detector 9 and near-infrared fluorescent channel detector 10 are formed detector module 18, the described flow chamber 4, beam splitting system 11 of drawing together formed a point-to-point imaging system 17 with detector module 18, and the optical module that being observed of described flow chamber 4 a little can be passed through on the beam splitting system 11 is respectively in the light-sensitive surface imaging of lateral scattering detector 8, yellow fluorescence channel detector 9, near-infrared fluorescent channel detector 10.Be observed the scattered light of cell, faint fluorescence can better converge on the detector light-sensitive surface through described optical module, finishes the detection of feeble signal.
The foregoing description just is to allow the one of ordinary skilled in the art can understand content of the present utility model and enforcement according to this for technical conceive of the present utility model and characteristics being described, its objective is, can not limit protection domain of the present utility model with this.The variation or the modification of every equivalence of having done according to the essence of the utility model content all should be encompassed in the protection domain of the present utility model.
Claims (4)
1. flow cytometer, comprise source illumination system (15), liquid fluid system (16), beam splitting system (11), acquisition of signal analysis process system (14) and PC display control program (13), described liquid fluid system (16) makes cell sample make streamlined flow under the parcel of sheath stream, described source illumination system (15) carries out laser radiation to the interested stream of cells of fluorochrome label in the described liquid fluid system (16), excite transmitting of the fluorescence that obtains institute's mark, enter described acquisition of signal analysis process system (14) by described beam splitting system (11) and carry out acquisition of signal, analyze and output, described PC display control program (13) shows the information of described acquisition of signal analysis process system (14) and stores and the described source illumination system of FEEDBACK CONTROL (15), liquid fluid system (16) and acquisition of signal analysis process system (14), described liquid fluid system (16) comprises flow chamber (4), the cell (5) that has streamlined flow in the described flow chamber (4), one solid state laser (1) is as the excitation source of source illumination system, laser is through catoptron (2), plano-convex lens (3) is assembled and is shone on the flow chamber (4), further assemble through the opticator that is integrated in flow chamber (4), make laser radiation on the cell (5) of streamlined flow, it is characterized in that: described acquisition of signal analysis process system comprises a lateral scattering detector (8) that is separately positioned on after the described beam splitting system (11), an one yellow fluorescence channel detector (9) and a near-infrared fluorescent channel detector (10), also comprise a forward scattering detector (7), and one connects described forward scattering detector (7), lateral scattering detector (8), the signal processing module (12) of yellow fluorescence channel detector (9) and near-infrared fluorescent channel detector (10).
2. flow cytometer according to claim 1 is characterized in that: also be provided with a light barrier (6) between described forward scattering detector (7) and the described liquid fluid system (16).
3. flow cytometer according to claim 1, it is characterized in that: described lateral scattering detector (8), yellow fluorescence channel detector (9) and near-infrared fluorescent channel detector (10) are formed detector module (18), and described flow chamber (4), beam splitting system (11) are formed a point-to-point imaging system (17) with detector module (18).
4. according to claim 1 or 2 or 3 described flow cytometers, it is characterized in that: described forward scattering detector (7), lateral scattering detector (8), yellow fluorescence channel detector (9) and near-infrared fluorescent channel detector (10) are all many pixels photon counter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020613651 CN201917509U (en) | 2010-11-18 | 2010-11-18 | Flow cytometry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020613651 CN201917509U (en) | 2010-11-18 | 2010-11-18 | Flow cytometry |
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| CN201917509U true CN201917509U (en) | 2011-08-03 |
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| CN 201020613651 Expired - Lifetime CN201917509U (en) | 2010-11-18 | 2010-11-18 | Flow cytometry |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102087198A (en) * | 2010-11-18 | 2011-06-08 | 苏州生物医学工程技术研究所 | Flow cytometry |
| CN103048258A (en) * | 2012-12-26 | 2013-04-17 | 江西科技师范大学 | Light splitting device for flow cytometer |
| CN104502255A (en) * | 2014-12-29 | 2015-04-08 | 中国科学院长春光学精密机械与物理研究所 | Three-dimensional imaging flow cytometer device |
| US9746412B2 (en) | 2012-05-30 | 2017-08-29 | Iris International, Inc. | Flow cytometer |
| CN108107025A (en) * | 2017-11-24 | 2018-06-01 | 中国科学院苏州生物医学工程技术研究所 | A kind of water quality detection method and system |
| TWI635279B (en) * | 2018-01-23 | 2018-09-11 | 黃國陽 | The methods of detecting cancer stem cells in vitro |
-
2010
- 2010-11-18 CN CN 201020613651 patent/CN201917509U/en not_active Expired - Lifetime
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102087198A (en) * | 2010-11-18 | 2011-06-08 | 苏州生物医学工程技术研究所 | Flow cytometry |
| US9746412B2 (en) | 2012-05-30 | 2017-08-29 | Iris International, Inc. | Flow cytometer |
| US10126227B2 (en) | 2012-05-30 | 2018-11-13 | Iris International, Inc. | Flow cytometer |
| US10209174B2 (en) | 2012-05-30 | 2019-02-19 | Iris International, Inc. | Flow cytometer |
| US10330582B2 (en) | 2012-05-30 | 2019-06-25 | Iris International, Inc. | Flow cytometer |
| US11255772B2 (en) | 2012-05-30 | 2022-02-22 | Iris International, Inc. | Flow cytometer |
| US11703443B2 (en) | 2012-05-30 | 2023-07-18 | Iris International, Inc. | Flow cytometer |
| CN103048258A (en) * | 2012-12-26 | 2013-04-17 | 江西科技师范大学 | Light splitting device for flow cytometer |
| CN104502255A (en) * | 2014-12-29 | 2015-04-08 | 中国科学院长春光学精密机械与物理研究所 | Three-dimensional imaging flow cytometer device |
| CN104502255B (en) * | 2014-12-29 | 2017-04-05 | 中国科学院长春光学精密机械与物理研究所 | Three-dimensional imaging flow cytometry device |
| CN108107025A (en) * | 2017-11-24 | 2018-06-01 | 中国科学院苏州生物医学工程技术研究所 | A kind of water quality detection method and system |
| TWI635279B (en) * | 2018-01-23 | 2018-09-11 | 黃國陽 | The methods of detecting cancer stem cells in vitro |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20151218 Address after: Science and Technology City kolding road high tech Zone of Suzhou City, Jiangsu Province, No. 88 215000 Patentee after: SUZHOU INSTITUTE OF BIOMEDICAL ENGINEERING AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES Address before: 215000 No. 14 Longshan Road, hi tech Zone, Jiangsu, Suzhou Patentee before: Suzhou Institute of Biomedical Engineering and Technology |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160323 Address after: Science and Technology City kolding road high tech Zone of Suzhou City, Jiangsu Province, No. 88 215163 Patentee after: SUZHOU INSTITUTE OF BIOMEDICAL ENGINEERING AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES Address before: Science and Technology City kolding road high tech Zone of Suzhou City, Jiangsu Province, No. 88 215000 Patentee before: SUZHOU INSTITUTE OF BIOMEDICAL ENGINEERING AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES |
|
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Zhongsheng (Suzhou) Medical Instrument Co., Ltd. Assignor: SUZHOU INSTITUTE OF BIOMEDICAL ENGINEERING AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES Contract record no.: 2017320010008 Denomination of utility model: Flow cytometry Granted publication date: 20110803 License type: Exclusive License Record date: 20170307 |
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| EE01 | Entry into force of recordation of patent licensing contract | ||
| CX01 | Expiry of patent term |
Granted publication date: 20110803 |
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| CX01 | Expiry of patent term |