CN102590168A - Light emitting diode-based fluorescent microorganism detector - Google Patents
Light emitting diode-based fluorescent microorganism detector Download PDFInfo
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- CN102590168A CN102590168A CN2012100349687A CN201210034968A CN102590168A CN 102590168 A CN102590168 A CN 102590168A CN 2012100349687 A CN2012100349687 A CN 2012100349687A CN 201210034968 A CN201210034968 A CN 201210034968A CN 102590168 A CN102590168 A CN 102590168A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
Abstract
The invention relates to a light emitting diode-based fluorescent microorganism detector which comprises a light source and is characterized in that the light source is a light emitting diode light source; an emitted light collecting and focusing system, an air flow pipeline, a fluorescent focusing system and a photoelectric detector are sequentially arranged in the spreading direction of emitted light of the light source; the emitted light of the light source is collected and focused by the emitted light collecting and focusing system to form a focused light spot; the focused light spot is positioned on the air flow pipeline to excite endogenous fluorescent substances in a microbial cell in the detected gas in the air flow pipeline to ensure that the endogenous fluorescent substances produce fluorescent signals so as to form a fluorescent light source; and the fluorescent focusing system is arranged on the side of the air flowing pipeline and is used for collecting the fluorescent signals produced through excitation and focusing the fluorescent signals on the photoelectric detector. A light emitting diode is used as an excitation light source of the endogenous fluorescent substances of microorganism, an air microorganism can be detected in real time, and manufacturing cost is very low.
Description
Technical field
The invention belongs to environmental microorganism detects and the pollution control technology field; Relate to a kind of air microbe (bacterium and fungi) detection technique of utilizing light emitting diode (LED) as the excitation source of the endogenous fluorescent material of microorganism, specifically a kind of fluorescence microorganism detection appearance based on light emitting diode.
Background technology
In the prior art, many production environments need be carried out strict environmental contaminants (comprising dust particle and bacterium) control.In the relevant industry of public health, such as, in pharmacy and medicine equipment production environment, not only need control airborne total population, also need control the number of bacterium and fungi simultaneously.Microorganism can cause medicine (apparatus) to be polluted and produce the potential risk of patient harm.And, contamination accident is detected in real time, obtain the instant messages of pollution source, affirmation is biological pollution or abiotic pollution, and is of crucial importance in the production of pharmaceutical factory or medical apparatus and instruments factory.
Traditional microorganism detection means are to gather air microbe through microorganism sampler, cultivate through nutrient culture media and differentiate after 3-5 days again.The cultivation back detection delay during this period of time of control from the collection microorganism to to(for) production run is a very big problem.Now, new microorganism real-time detection method is introduced into the production environment controlling unit gradually.Such as; The optical detecting instrument that is used to detect microorganism autofluorescence material (as: reduced coenzyme NADH, lactochrome) emerges; These optical detecting instruments possess the advantage that provides the microbial contamination testing result immediately, and this advantage makes the health care relevant industries benefited a great deal.
These air microbe optical detector major parts are to use laser diode as light source, and these light sources possess suitable wavelengths, possess good excitation for the fluorescent material in the microbial body.But these laser diode light sources are very expensive.From reducing cost, the angle that prolongs light source life is set out, and using light emitting diode (LED) to substitute laser diode is a better choice as the fluorescence excitation light source in the microorganism detection process.
Compare with laser diode; The luminous energy diffusion of light emitting diode is wider; If will use light emitting diode as microorganism detection process fluorescence excitation light source; Need carry out some special optical touch to light emitting diode, the emission light gathering that light emitting diode is dispersed is to produce a light beam that can effectively excite bioluminescence.
Summary of the invention
The objective of the invention is to overcome the deficiency that exists in the prior art; A kind of fluorescence microorganism detection appearance based on light emitting diode is provided; This detector utilizes the excitation source of light emitting diode (LED) as the endogenous fluorescent material of microorganism; Can detect immediately air microbe (bacterium and fungi), and manufacturing cost is lower.
According to technical scheme provided by the invention: based on the fluorescence microorganism detection appearance of light emitting diode; Comprise light source; It is characterized in that: said light source adopts LED source, on the direction of propagation of source emissioning light, is disposed with emission light gathering focusing system, airflow pipeline, fluorescence lens system and photoelectric detector, and the emission light of said light source is collected through the emission light gathering focusing system and is focused to a focal spot; And this focal spot is positioned on the said airflow pipeline; To excite the endogenous fluorescent material in the microbial cell in the detected gas in the airflow pipeline, make the endogenous fluorescent material produce fluorescence signal, form fluorescence light source; Said fluorescence lens system is arranged at the airflow pipeline side, and the fluorescence lens system is used to assemble the fluorescence signal that excites generation and focuses it on photoelectric detector.
As further improvement of the present invention, said emission light gathering focusing system comprises the collecting lens and emission light focus lens group that is provided with in order along emission light transmit direction, and said LED source is positioned on the primary optical axis of collecting lens.
As further improvement of the present invention, said collecting lens adopts wide-angle lens, fish eyes lens or meniscus lens, 70% ~ 90% the emission light that it can collect that LED source sends.
As further improvement of the present invention, said emission light focus lens group comprises at least one condenser lens, and it is used for the emission light that collecting lens is collected is converged is a focal spot.
As further improvement of the present invention, said LED source front end is provided with optical filter, and this optical filter can transmit the emission light of the 350-420nm nominal wavelength of LED source, and can filter out the emission light longer than nominal wavelength; Be provided with the emission light that is used for the filtering LED source before the said photoelectric detector, the interference filter that lets fluorescence pass through simultaneously.Preferably, said optical filter and interference filter all adopt interference filter.
As further improvement of the present invention, said fluorescence lens system comprises ellipsoidal reflector, and said fluorescence light source is positioned on the focus of said ellipsoidal reflector, and another focus of said ellipsoidal reflector is positioned on the said photoelectric detector.
As further improvement of the present invention; Said fluorescence lens system comprises parabolic mirror and fluorescence focus lens group; Said fluorescence light source is positioned on the focus of said parabolic mirror; The fluorescence that fluorescence light source sends forms the fluorescent light beam of collimation after parabolic mirror reflects, this fluorescent light beam focuses on the said photoelectric detector through the fluorescence focus lens group.
Said fluorescence focus lens group comprises at least one condenser lens.
As further improvement of the present invention, said photoelectric detector adopts photomultiplier.
The present invention compared with prior art, advantage is:The present invention utilizes the excitation source of light emitting diode (LED) as the endogenous fluorescent material of microorganism, can detect immediately air microbe (bacterium and fungi), and manufacturing cost is lower.
Description of drawings
Fig. 1 is the structural representation of the embodiment of the invention 1.
Fig. 2 is the structural representation of the embodiment of the invention 2.
Fig. 3 is one of structural representation of emission light gathering focusing system (collecting lens is a wide-angle lens, and emission light focus lens group is single condenser lens).
Fig. 4 is two (collecting lens is a wide-angle lens, and emission light focus lens group is a plurality of condenser lenses) of the structural representation of emission light gathering focusing system.
Fig. 5 is three (collecting lens is a meniscus lens, and emission light focus lens group is single condenser lens) of the structural representation of emission light gathering focusing system.
Fig. 6 is four (collecting lens is a meniscus lens, and emission light focus lens group is a plurality of condenser lenses) of the structural representation of emission light gathering focusing system.
Embodiment
Below in conjunction with concrete accompanying drawing and embodiment the present invention is described further.
Embodiment 1
As shown in Figure 1; The fluorescence microorganism detection appearance of present embodiment adopts light emitting diode (LED) light source 1, on the LED source 1 radiative direction of propagation, is disposed with emission light gathering focusing system, airflow pipeline 4, fluorescence lens system, interference filter 6 and photoelectric detector 7.
The emission light of said LED source 1 is collected through the emission light gathering focusing system and is focused to a focal spot; And this focal spot is positioned on the said airflow pipeline 4; To excite the endogenous fluorescent material in the microbial cell in the detected gas in the airflow pipeline 4; Make the endogenous fluorescent material produce fluorescence signal, form fluorescence light source; Said fluorescence lens system is arranged at airflow pipeline 4 sides, and the fluorescence lens system is used to assemble the fluorescence signal that excites generation and focuses it on photoelectric detector 7.
For through exciting the endogenous fluorescent material (metabolin and other biological molecule) in the microbial cell to carry out microorganism detection effectively, need the excitating light strength of certain level.Consider that from practical experience it is first-selected direction that light beam is focused on a certain set mid point.The present invention has selected LED source 1 for use; Because LED source 1 has the emission light of a wide-angle usually; Therefore need to use an emission light gathering focusing system to collect the diverging light of light emitting diode as much as possible, and make it form the focal spot of concentrating.And the emission light gathering focusing system need possess light capacity gauge efficiently.
In the present embodiment, said emission light gathering focusing system such as Fig. 1, shown in Figure 3, it comprises the collecting lens 2 and emission light focus lens group 3 that is provided with in order along emission light transmit direction, said LED source 1 is positioned on the primary optical axis of collecting lens 2.Said collecting lens 2 adopts wide-angle lenses, 70% ~ 90% the emission light that it can collect that LED source 1 sends; The effect of said emission light focus lens group 3 is beam shapes that finishing collecting lens 2 is collected, to reach the purpose that in a small volume regions, forms high strength luminous energy.Emission light focus lens group 3 in the present embodiment has adopted single condenser lens.
Because the autofluorescence of bacterium is very faint, the fluorescence lens system that when design, is necessary to use other is assembled the fluorescence signal that excites generation and is focused it on the sensitive photoelectric detector 7.In the present embodiment, described fluorescence lens system is an ellipsoidal reflector 5, and said fluorescence light source is positioned on the focus of said ellipsoidal reflector 5, and another focus of said ellipsoidal reflector 5 is positioned on the said photoelectric detector 7.
During concrete the application; Converging after the emission light that sends from LED source 1 is collected via the emission light gathering focusing system is a focal spot; This focal spot focuses on the airflow pipeline 4; Because the detected gas circulation is arranged in the airflow pipeline 4, and the endogenous fluorescent material in the microbial cell in the said detected gas can be excited under the focal spot irradiation, formed fluorescence light source.Because this fluorescence light source is positioned on the focus of said ellipsoidal reflector 5; After reflecting through ellipsoidal reflector 5, fluorescence converges at another focus of ellipsoidal reflector 5; Promptly converge on the sensitive photoelectric detector 7 (like photomultiplier), detecting processing through follow-up treatment facility then.
In practical operation, what be used for microorganism detection effectively excites light wavelength between the 350-420 nanometer.But, be different from the light emitting diode single wavelength of nominal because the radiative wavelength of LED source 1 has the distribution of wider range usually.The emission light that these wavelength are mixed and disorderly, especially longer than nominal wavelength (350-420 nanometer) light is noisy for fluoroscopic examination, need exclude.For this reason; Said LED source 1 front end preferably is provided with an optical filter (not drawing among the figure); This optical filter preferably adopts interference filter, and it can transmit LED source 1 nominal wavelength, also can filter out the emission light longer than light emitting diode nominal wavelength.
In addition; In order to guarantee the accuracy of photoelectric detector 7 testing results; The present invention also is provided with the emission light that is used for filtering LED source 1 before photoelectric detector 7, the interference filter 6 that lets fluorescence pass through simultaneously, and said interference filter 6 also preferably adopts interference filter.
As shown in Figure 2, present embodiment is compared with embodiment 1, and difference only is that the fluorescence lens system is different.Fluorescence lens system among the embodiment 2 comprises parabolic mirror 8 and fluorescence focus lens group 9; Said fluorescence light source is positioned on the focus of said parabolic mirror 8; The fluorescence that fluorescence light source sends forms the fluorescent light beam of collimation after parabolic mirror 8 reflections, this fluorescent light beam focuses on the said photoelectric detector 7 through fluorescence focus lens group 9.In the present embodiment, what described fluorescence focus lens group 9 adopted is single condenser lens, in actual use, also can adopt a plurality of condenser lens combinations.
During concrete the application; Converging after the emission light that sends from LED source 1 is collected via the emission light gathering focusing system is a focal spot; This focal spot focuses on the airflow pipeline 4; Because the detected gas circulation is arranged in the airflow pipeline 4, and the endogenous fluorescent material in the microbial cell in the said detected gas can be excited under the focal spot irradiation, formed fluorescence light source.Because this fluorescence light source is positioned on the focus of said parabolic mirror 8; Fluorescence forms parallel beam through parabolic mirror 8 reflection backs; This parallel beam is detecting processing through follow-up treatment facility then focusing on the photodetector 7 (like photomultiplier) through follow-up fluorescence focus lens group 9.
What should be understood that is, the foregoing description is just to explanation of the present invention, and can not be interpreted as limitation of the present invention, also has other feasible program among the present invention, for example:
Described emission light gathering focusing system can be the design that is embedded in before the light-emitting diode chip for backlight unit luminous zone, or is added on the light emitting diode of being furnished with supplementary lens and uses.
Collecting lens 2 in the said emission light gathering focusing system can also adopt fish eyes lens or meniscus lens (Fig. 5, shown in Figure 6), 70% ~ 90% the emission light that they can collect equally that LED source 1 sends.Said emission light focus lens group 3 can adopt single condenser lens, i.e. it is a focal spot (shown in Figure 3, shown in Figure 5) that the emission light of collecting lens 2 collections directly directly converges through single condenser lens; Said emission light focus lens group 3 can also adopt a plurality of condenser lenses, i.e. the emission light of collecting lens 2 collections transfers collimated light (Fig. 4, shown in Figure 6) to through a condenser lens earlier, and converging through other follow-up condenser lenses is a focal spot again.
Claims (10)
1. based on the fluorescence microorganism detection appearance of light emitting diode; Comprise light source (1); It is characterized in that: said light source (1) adopts LED source (1); On the radiative direction of propagation of light source (1), be disposed with emission light gathering focusing system, airflow pipeline (4), fluorescence lens system and photoelectric detector (7); The emission light of said light source (1) is collected through the emission light gathering focusing system and is focused to a focal spot, and this focal spot is positioned on the said airflow pipeline (4), to excite the interior endogenous fluorescent material of microbial cell in interior the detected gas of airflow pipeline (4); Make the endogenous fluorescent material produce fluorescence signal, form fluorescence light source; Said fluorescence lens system is arranged at airflow pipeline (4) side, and the fluorescence lens system is used to assemble the fluorescence signal that excites generation and focuses it on photoelectric detector (7).
2. the fluorescence microorganism detection appearance based on light emitting diode as claimed in claim 1; It is characterized in that: said emission light gathering focusing system comprises collecting lens (2) and the emission light focus lens group (3) that is provided with in order along emission light transmit direction, and said LED source (1) is positioned on the primary optical axis of collecting lens (2).
3. the fluorescence microorganism detection appearance based on light emitting diode as claimed in claim 2 is characterized in that: said collecting lens (2) adopts wide-angle lens, fish eyes lens or meniscus lens.
4. the fluorescence microorganism detection appearance based on light emitting diode as claimed in claim 3; It is characterized in that: said emission light focus lens group (3) comprises at least one condenser lens, and it is used for the emission light that collecting lens (2) is collected is converged is a focal spot.
5. the fluorescence microorganism detection appearance based on light emitting diode as claimed in claim 1; It is characterized in that: said LED source (1) front end is provided with optical filter; This optical filter can transmit the emission light of the 350-420nm nominal wavelength of LED source (1), and can filter out the emission light longer than nominal wavelength; The preceding emission light that is used for filtering LED source (1) that is provided with of said photoelectric detector (7), the interference filter (6) that lets fluorescence pass through simultaneously.
6. the fluorescence microorganism detection appearance based on light emitting diode as claimed in claim 5, it is characterized in that: said optical filter and interference filter (6) all adopt interference filter.
7. the fluorescence microorganism detection appearance based on light emitting diode as claimed in claim 1; It is characterized in that: said fluorescence lens system comprises ellipsoidal reflector (5); Said fluorescence light source is positioned on the focus of said ellipsoidal reflector (5), and another focus of said ellipsoidal reflector (5) is positioned on the said photoelectric detector (7).
8. the fluorescence microorganism detection appearance based on light emitting diode as claimed in claim 1; It is characterized in that: said fluorescence lens system comprises parabolic mirror (8) and fluorescence focus lens group (9); Said fluorescence light source is positioned on the focus of said parabolic mirror (8); The fluorescence that fluorescence light source sends forms the fluorescent light beam of collimation after parabolic mirror (8) reflection, this fluorescent light beam focuses on the said photoelectric detector (7) through fluorescence focus lens group (9).
9. the fluorescence microorganism detection appearance based on light emitting diode as claimed in claim 8, it is characterized in that: said fluorescence focus lens group (9) comprises at least one condenser lens.
10. the fluorescence microorganism detection appearance based on light emitting diode as claimed in claim 1 is characterized in that: said photoelectric detector (7) adopts photomultiplier.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105039148A (en) * | 2015-07-02 | 2015-11-11 | 济南大学 | Device for detecting, filtering, and gathering microbes in air |
| CN105510297A (en) * | 2015-12-29 | 2016-04-20 | 北京华泰诺安探测技术有限公司 | Raman fluorescence spectrum testing system and optical signal collector thereof |
| CN105642376A (en) * | 2015-12-29 | 2016-06-08 | 南京邮电大学 | Bioluminescent micro-total analysis system chip and preparation method thereof |
| CN106596498A (en) * | 2017-01-19 | 2017-04-26 | 大连理工大学 | Device for quickly detecting air microorganisms |
| CN106770079A (en) * | 2016-11-17 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of fluorescent microorganism detector based on light emitting diode |
| CN108414487A (en) * | 2018-03-08 | 2018-08-17 | 北京航空航天大学 | A kind of optical collection method and system for solid outgoing fluorescence |
| CN108982459A (en) * | 2018-08-16 | 2018-12-11 | 中山路得斯空调有限公司 | Device for measuring floating microorganisms and air conditioner using device |
| CN109342031A (en) * | 2018-12-04 | 2019-02-15 | 桂林聚联科技有限公司 | A kind of light power meter receiving transducer |
| CN112345504A (en) * | 2020-10-30 | 2021-02-09 | 上海世艾生物科技有限公司 | Integrated fluorescence detection device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108414487A (en) * | 2018-03-08 | 2018-08-17 | 北京航空航天大学 | A kind of optical collection method and system for solid outgoing fluorescence |
| CN108982459A (en) * | 2018-08-16 | 2018-12-11 | 中山路得斯空调有限公司 | Device for measuring floating microorganisms and air conditioner using device |
| CN109342031A (en) * | 2018-12-04 | 2019-02-15 | 桂林聚联科技有限公司 | A kind of light power meter receiving transducer |
| CN112345504A (en) * | 2020-10-30 | 2021-02-09 | 上海世艾生物科技有限公司 | Integrated fluorescence detection device |
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Application publication date: 20120718 |