CN205893243U - Eccentric wobbling nucleic acid extraction equipment - Google Patents

Eccentric wobbling nucleic acid extraction equipment Download PDF

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Publication number
CN205893243U
CN205893243U CN201620919936.9U CN201620919936U CN205893243U CN 205893243 U CN205893243 U CN 205893243U CN 201620919936 U CN201620919936 U CN 201620919936U CN 205893243 U CN205893243 U CN 205893243U
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China
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bar magnet
eccentric
tube rack
test tube
nucleic acid
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CN201620919936.9U
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骆广进
程志
王伟平
骆志成
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Hangzhou Ao Sheng Instrument Ltd
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Hangzhou Ao Sheng Instrument Ltd
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Abstract

The utility model relates to an eccentric wobbling nucleic acid extraction equipment, including the base, set up test -tube rack, bar magnet stock and bar magnet frame on the base, test -tube rack actuating mechanism, bar magnet cover actuating mechanism and the bar magnet actuating mechanism of separately -driven test -tube rack, magnetosheath frame and the independent action of bar magnet frame, its characterized in that: test -tube rack actuating mechanism's output shaft is the eccentric shaft, the eccentric shaft drive the eccentric rotation is done to the test -tube rack, the power take off end has on bar magnet cover actuating mechanism and the bar magnet actuating mechanism respectively, power take off holds separately -driven the perpendicular to is done to bar magnet stock and bar magnet frame test -tube rack plane of rotation's direction back and forth movement. Its effect: the nucleic acid extraction great to the sample volume works, adopts to eccentric driving mechanism, drives plane direction or direction in space were done to the test -tube rack circumference rotation or rotation of curve mode or the round swing of linearity / nonlinearity, can make nucleic acid and magnetic bead intensive mixing, can improve extraction percentage and extraction efficiency.

Description

The nucleic acid extraction instrument of eccentric swing
Technical field
This utility model is related to a kind of nucleic acid extraction instrument, more particularly, to a kind of nucleic acid extraction instrument of eccentric swing.
Background technology
Extract nucleic-acid manipulation in paramagnetic particle method, whether fully magnetic bead and free nucleic acid mix, whether completely bar magnet adsorbs magnetic bead, Most important to nucleic acid extraction effect, the magnetic bead of particularly substantially product sample mixes operation and magnetic bead absorption, particularly important.For For the nucleic acid extraction work of conventional low capacity, when extracting nucleic acid, pumped by bar magnet, bar magnet covers gyration, And by the imbibition of pipettor, draining mode, so that magnetic bead and free nucleic acid is mixed, magnetic bead adsorbs nucleic acid, then bar magnet is by magnetic Pearl absorption covers outer wall in bar magnet, completes a step of nucleic acid extraction work.
Say for extracting the big work of sample volume, when extracting nucleic acid, after adding lysate, buffer and magnetic bead liquid, always Liquid volume bigger, need the sample tube with large volume, be difficult to make large volume sample liquid and magnetic bead to mix fully, with general Logical nucleic acid extracting instrument is difficult to extract the work of nucleic acid completely: extraction nucleic acid is thorough or needs increase extraction time to carry out not Repeat to mix disconnectedly, extract work, have influence on extraction ratio and extraction efficiency.
Content of the invention
The purpose of this utility model is to solve the problems referred to above of prior art presence and provides a kind of core of eccentric swing Sour extraction equipment, particularly with sample volume larger nucleic acid extraction work, using test tube rack drive mechanism and be eccentric drive Mechanism, drive test tube rack does the circular-rotation of in-plane or direction in space or curve mode rotates or linear/non-linear is past Return swing, and described bar magnet stock and bar magnet frame do the direction back and forth movement perpendicular to described test tube rack plane of rotation, including vertical In test tube inside, back and forth movement outward, also include perpendicular to test tube inside, outward back and forth movement while, with the axis of test tube Centered on axle rotate, nucleic acid and magnetic bead can be made to be sufficiently mixed, it is possible to increase extraction ratio and extraction efficiency.
Above-mentioned technical purpose of the present utility model mainly solves by the following technical programs: the nucleic acid of eccentric swing carries Taking equipment, including pedestal, is arranged on test tube rack on pedestal, bar magnet stock and bar magnet frame, drive respectively test tube rack, magnetosheath frame and The test tube rack drive mechanism of bar magnet frame self contained function, bar magnet set drive mechanism and bar magnet drive mechanism it is characterised in that: described examination The output shaft of pipe support drive mechanism is eccentric shaft, and described eccentric shaft drives described test tube rack to do eccentric rotary, and described bar magnet set drives Be respectively provided with clutch end on motivation structure and bar magnet drive mechanism, described clutch end drive respectively described bar magnet stock and Bar magnet frame does the direction back and forth movement perpendicular to described test tube rack plane of rotation.For the nucleic acid extraction work that sample volume is larger, Using test tube rack drive mechanism and be eccentric drive mechanism, drive test tube rack do in-plane or direction in space circular-rotation or Curve mode rotates or linear/non-linear reciprocating swing, and described bar magnet stock and bar magnet frame do perpendicular to described test tube rack The direction back and forth movement of plane of rotation, including perpendicular to test tube inside, back and forth movement outward, also include perpendicular to test tube inside, past While outer back and forth movement, centered on the axis of test tube, axle rotates, can make substantially product sample nucleic acid (and cracking Liquid etc.) and magnetic bead be sufficiently mixed, magnetic bead fully adsorbs nucleic acid, it is possible to increase extraction ratio and extraction efficiency.
Additionally, the technical program is equally applicable to the operation of the nucleic acid extraction process such as follow-up washing and eluting.
As improving further and supplementing to technique scheme, this utility model adopts following technical measures: described Test tube rack drive mechanism includes the motor being arranged on pedestal, by described motor-driven eccentric stiffener, on described eccentric stiffener Eccentric shaft outfan connect described test tube rack, drive described test tube rack do eccentric rotary.Eccentric stiffener drives test tube rack to move Make, with test tube rack synchronization action, high speed motion is used for magnetic bead and mixes sample tube, and low speed action is used for adsorbing magnetic bead.
Described eccentric stiffener includes the output wheel being arranged on motor output shaft, and the driving wheel being driven by output wheel is described Driving wheel drives eccentric shaft turns.
Wherein, drive driving wheel action with regard to output wheel, at least include following two schemes, any scheme is all can To implement:
The first scheme: described output wheel is gear, drives described driving wheel by change-speed gearing group;
Second scheme: described output wheel is belt wheel, drives described driving wheel by conveyer belt.Wherein, belt wheel is belt Or chain.It is output belt pulley and driving belt pulley with belt cooperation;It is output gear and driving gear with chain interoperation.
Described driving wheel is provided with balance weight, for keeping described driving wheel smooth rotation.Balance weight is used for balancing bias The eccentric amount of unbalance of mechanism, test tube rack steadily swings, and meanwhile, driving wheel will not be followed Spin tubes frame action and be produced eccentric Vibration.
Described eccentric shaft includes drive portion, positioned at lower fixed part and the upper drive division of drive division both sides, described lower fixed part It is fixed on described pedestal by bearing, described upper drive division connects a dynamic gripper shoe by bearing, and described dynamic gripper shoe sets Put described test tube rack.Eccentric shaft is structure as a whole, and lower fixed part and upper drive division, respectively by bearings, make eccentric shaft spacing Pedestal rotates.Test tube rack is connected with eccentric stiffener by dynamic gripper shoe, and test tube rack overall structure can be made to simplify, it is to avoid There is the substrate of high intensity.Due to the test tube position on test tube rack size according to test tube depending on, therefore there is different size Test tube rack, after the technical program, the overall structure of test tube rack is relatively reasonable, is convenient for changing, and is also convenient for reduces cost.
Described dynamic gripper shoe bottom arranges a retainer ring, described upper drive division spacing in described retainer ring, described upper drive Bearing in dynamic portion is a clutch shaft bearing, and the bearing on described lower fixed part is two spaced bearings, respectively the Two bearings and 3rd bearing.Racking gripper shoe is propped up using the scheme of three bearings so that dynamic gripper shoe only eccentric action, not produce Raw revolution action.
Some fixing pillars are arranged on described pedestal, described fixing pillar is distributed in encirclement mode, on described fixing pillar Setting is outer to polarize ring, polarization ring in setting in described outer polarization ring, and described interior polarization ring is fixed on described fixation by connecting plate On ring, described interior polarization ring passes through on the first spring leaf fixing polarization outside ring, and it is solid that described outer polarization ring passes through second spring piece It is scheduled on described fixing pillar.
Also include electronic control system, described electronic control system is driven with described motor, bar magnet drive mechanism and bar magnet set Motivation structure signal connects, for controlling described test tube rack, magnetosheath frame and bar magnet frame action respectively.Accurately controlled by electronic control system Make each drive mechanism precise movement, finally improve the degree of accuracy of instrument for extracting nucleic acid work.When being embodied as, on motor or Motor speed sensor is arranged on pedestal, speed probe is used for detecting the rotating speed of described motor, and rotating speed is sent to electronics Control system, electronic control system sends instruction controlled motor according to rotary speed information and appropriately works.
Also include attemperating unit, for invisible spectro in temperature control and/or test tubes frameLiquidTemperature.Make invisible spectro liquid Temperature is appropriate problem, meets the requirement extracted, it is to avoid temperature of liquid is too high or too low, is conducive to improving extraction efficiency and carries Take rate.
This utility model has the advantages that 1, for the larger nucleic acid extraction work of sample volume, using examination Pipe support drive mechanism and be eccentric drive mechanism, drives test tube rack to do circular-rotation or the curve side of in-plane or direction in space Formula rotates or linear/non-linear reciprocating swing, and described bar magnet stock and bar magnet frame do perpendicular to described test tube rack plane of rotation Direction back and forth movement, including perpendicular to test tube inside, back and forth movement outward, also include coming and going inside, outward perpendicular to test tube While motion, centered on the axis of test tube, axle rotates, and nucleic acid and magnetic bead can be made to be sufficiently mixed, it is possible to increase carry Take rate and extraction efficiency.2nd, in test tube rack drive mechanism, setting balance weight is used for balancing the eccentric amount of unbalance of eccentric stiffener, examination Pipe support steadily swings, and meanwhile, driving wheel will not be followed Spin tubes frame action and be produced eccentric vibrating.3rd, eccentric shaft adopts three The scheme of bearing, to prop up racking gripper shoe so that dynamic gripper shoe only eccentric action, does not produce revolution action.4th, by Electronic Control system System each drive mechanism precise movement of precise control, finally improves the degree of accuracy of instrument for extracting nucleic acid work, when being embodied as, electricity Motor speed sensor is set on machine or on pedestal, and speed probe is used for detecting the rotating speed of described motor, and rotating speed is sent out Deliver to electronic control system, electronic control system sends instruction controlled motor according to rotary speed information and appropriately works.5. temperature control is set Device, for invisible spectro temperature of liquid in temperature control and/or test tubes frame, makes invisible spectro temperature of liquid be appropriate problem, Meet the requirement extracted, it is to avoid temperature of liquid is too high or too low, be conducive to improving extraction efficiency and extraction ratio.
Brief description
Fig. 1 is the structural representation of the compositions such as each drive mechanism in this utility model, test tube rack, bar magnet frame, bar magnet stock Figure.
Fig. 2 is the cross section structure diagram of test tube rack drive mechanism in this utility model.
Fig. 3 is the structural representation of test tube rack drive mechanism cooperation polarization ring in this utility model.
Specific embodiment
Below by embodiment, and combine accompanying drawing, the technical solution of the utility model is described in further detail.
Embodiment: the nucleic acid extraction instrument of eccentric swing, including pedestal, it is arranged on test tube rack on pedestal, bar magnet stock With bar magnet frame, drive test tube rack drive mechanism, the bar magnet set drive mechanism of test tube rack, magnetosheath frame and bar magnet frame self contained function respectively With bar magnet drive mechanism it is characterised in that: the output shaft of described test tube rack drive mechanism is eccentric shaft, and described eccentric shaft drives institute State test tube rack and do eccentric rotary, described bar magnet set drive mechanism and bar magnet drive mechanism are respectively provided with clutch end, described Clutch end drives described bar magnet stock and bar magnet frame to do the direction back and forth movement perpendicular to described test tube rack plane of rotation respectively. For sample volume larger nucleic acid extraction work, using test tube rack drive mechanism and be eccentric drive mechanism, drive test tube rack Do in-plane or the circular-rotation of direction in space or curve mode rotates or linear/non-linear reciprocating swing, and described magnetic Excellent stock and bar magnet frame do the direction back and forth movement perpendicular to described test tube rack plane of rotation, including perpendicular to test tube inside, outward Back and forth movement, also include perpendicular to test tube inside, outward back and forth movement while, centered on the axis of test tube axle do rotation fortune Dynamic, the nucleic acid (and lysate etc.) of substantially product sample and magnetic bead can be made to be sufficiently mixed, magnetic bead fully adsorbs nucleic acid, it is possible to increase Extraction ratio and extraction efficiency.
Additionally, the technical program is equally applicable to the operation of the nucleic acid extraction process such as follow-up washing and eluting.
As improving further and supplementing to technique scheme, this utility model adopts following technical measures: described Test tube rack drive mechanism includes the motor being arranged on pedestal, by described motor-driven eccentric stiffener, on described eccentric stiffener Eccentric shaft outfan connect described test tube rack, drive described test tube rack do eccentric rotary.Eccentric stiffener drives test tube rack to move Make, with test tube rack synchronization action, high speed motion is used for magnetic bead and mixes sample tube, and low speed action is used for adsorbing magnetic bead.
Described eccentric stiffener includes the output wheel being arranged on motor output shaft, and the driving wheel being driven by output wheel is described Driving wheel drives eccentric shaft turns.
Wherein, drive driving wheel action with regard to output wheel, at least include following two schemes, any scheme is all can To implement:
The first scheme: described output wheel is gear, drives described driving wheel by change-speed gearing group;
Second scheme: described output wheel is belt wheel, drives described driving wheel by conveyer belt.Wherein, belt wheel is belt Or chain.It is output belt pulley and driving belt pulley with belt cooperation;It is output gear and driving gear with chain interoperation.
Described driving wheel is provided with balance weight, for keeping described driving wheel smooth rotation.Balance weight is used for balancing bias The eccentric amount of unbalance of mechanism, test tube rack steadily swings, and meanwhile, driving wheel will not be followed Spin tubes frame action and be produced eccentric Vibration.
Described eccentric shaft includes drive portion, positioned at lower fixed part and the upper drive division of drive division both sides, described lower fixed part It is fixed on described pedestal by bearing, described upper drive division connects a dynamic gripper shoe by bearing, and described dynamic gripper shoe sets Put described test tube rack.Eccentric shaft is structure as a whole, and lower fixed part and upper drive division, respectively by bearings, make eccentric shaft spacing Pedestal rotates.Test tube rack is connected with eccentric stiffener by dynamic gripper shoe, and test tube rack overall structure can be made to simplify, it is to avoid There is the substrate of high intensity.Due to the test tube position on test tube rack size according to test tube depending on, therefore there is different size Test tube rack, after the technical program, the overall structure of test tube rack is relatively reasonable, is convenient for changing, and is also convenient for reduces cost.
Described dynamic gripper shoe bottom arranges a retainer ring, described upper drive division spacing in described retainer ring, described upper drive Bearing in dynamic portion is a clutch shaft bearing, and the bearing on described lower fixed part is two spaced bearings, respectively the Two bearings and 3rd bearing.Racking gripper shoe is propped up using the scheme of three bearings so that dynamic gripper shoe only eccentric action, not produce Raw revolution action.
Some fixing pillars are arranged on described pedestal, described fixing pillar is distributed in encirclement mode, on described fixing pillar Setting is outer to polarize ring, polarization ring in setting in described outer polarization ring, and described interior polarization ring is fixed on described fixation by connecting plate On ring, described interior polarization ring passes through on the first spring leaf fixing polarization outside ring, and it is solid that described outer polarization ring passes through second spring piece It is scheduled on described fixing pillar.
Also include electronic control system, described electronic control system is driven with described motor, bar magnet drive mechanism and bar magnet set Motivation structure signal connects, for controlling described test tube rack, magnetosheath frame and bar magnet frame action respectively.Accurately controlled by electronic control system Make each drive mechanism precise movement, finally improve the degree of accuracy of instrument for extracting nucleic acid work.When being embodied as, on motor or Motor speed sensor is arranged on pedestal, speed probe is used for detecting the rotating speed of described motor, and rotating speed is sent to electronics Control system, electronic control system sends instruction controlled motor according to rotary speed information and appropriately works.
Also include attemperating unit, for invisible spectro in temperature control and/or test tubes frameLiquidTemperature.Make invisible spectro liquid Temperature is appropriate problem, meets the requirement extracted, it is to avoid temperature of liquid is too high or too low, is conducive to improving extraction efficiency and carries Take rate.
Now technique scheme is done and illustrates in detail:
As shown in figure 1, eight sample tube 1 on instrument for extracting nucleic acid, sample tube 1 is installed in test tube rack 2, and test tube rack 2 is fixing In dynamic gripper shoe 11, follow eccentric drive mechanism 13 eccentric action.
Bar magnet set 3 is installed on bar magnet stock 4, follows bar magnet set drive mechanism 8 and carries out action (parallel test tube axis direction Move to test tube bottom or move away from test tube direction, or the rotation with test tube axis as axle while mobile), it extend into When in sample tube 1, sample tube 1 bottom can be reached.
Bar magnet 5 is installed on bar magnet frame 6, follows bar magnet drive mechanism 7 and carries out action (inclusion parallel bar magnet set axis direction Move to test tube bottom or move away from test tube direction, or while mobile, the rotation as axle for the axis is covered with bar magnet), bar magnet 5 when stretching into bar magnet set 3, and covers 3 bottoms to bar magnet, and bar magnet set 3 can follow joint longitudinal moving mechanism 9(together with bar magnet 5 can together When drive bar magnet stock 4 action together with bar magnet frame 6) (parallel test tube axis direction is mobile or away from examination to test tube bottom for action Pipe direction is moved, or the rotation with test tube axis as axle while mobile).Reagent set 10 is installed in fixed plate 12.
As shown in Figures 2 and 3, the motor 23 on eccentric drive mechanism 13 is positioned pedestal 14 by fixed support 30 On, motor 23 direct drive is installed on the output pulley 15 on its motor shaft, drives driving pulley 17, drive belt by belt 16 Wheel 17 drive eccentric shaft 25 associated with it, the lower fixed part on eccentric shaft 25 is in the fixed seat 29 being arranged on pedestal Hole, and supported by second bearing 24 and 3rd bearing 26, fixed seat 29 is positioned on pedestal 14;The upper drive division of eccentric shaft 25 with It is provided with clutch shaft bearing 27, plane and the fixation of dynamic gripper shoe 11 in retainer ring 21 is connected, two row's sample tube between retainer ring 21 endoporus Frame 2 is then fixed in dynamic gripper shoe 11;Retainer ring 21 outer ring is connected by connecting plate with interior polarization ring 18, and interior polarization ring 18 passes through Spring leaf 22 is connected with outer polarization ring, and outer polarization ring 19 is passed through spring leaf 22 and is connected with fixing pillar 20, and balance weight 28 is used for putting down The amount of unbalance of weighing apparatus eccentric drive mechanism;Thus realizing being driven eight sample tube 1 action in dynamic gripper shoe 11 by motor 23, and Eccentric shaft rotation will not be followed and produce eccentric vibrating.
The eccentric diameter of eccentric shaft, passes through design according to sample pipe diameter, the bar magnet set correlative factor such as diameter and cumulative volume Calculate and determine.
Bar magnet set 3 and the translational speed of bar magnet 5, amplitude and position, all can be by electronic-controlled installation according to presetting Control.
In large volume sample nucleic acid extraction process, drive the action of sample tube 1 by eccentric drive mechanism 13, realize big Volume samples, lysate and magnetic bead mix, and magnetic bead absorption.
By the action of bar magnet set 3 and bar magnet 5, the nucleic acid extraction such as washing, eluting can be completed in the hole position of reagent set 10 Operating procedure.
In more than 2ml, the maximum volume that processes is 5ml to the large volume that the substantially product sample that this utility model is related to refers in sample More than.
The foregoing is only preferred embodiment of the present utility model, be not limited to this utility model.Above-mentioned In embodiment, this utility model can have various modifications and variations.All of the present utility model spirit and principle within, made Any modification, equivalent substitution and improvement etc., should be included within protection domain of the present utility model.

Claims (10)

1. the nucleic acid extraction instrument of eccentric swing, including pedestal, is arranged on test tube rack on pedestal, bar magnet stock and bar magnet frame, The test tube rack drive mechanism, bar magnet set drive mechanism and the bar magnet that drive test tube rack, magnetosheath frame and bar magnet frame self contained function respectively drive Motivation structure it is characterised in that: the output shaft of described test tube rack drive mechanism is eccentric shaft, and described eccentric shaft drives described test tube rack Do eccentric rotary, described bar magnet set drive mechanism and bar magnet drive mechanism are respectively provided with clutch end, described power output End drives described bar magnet stock and bar magnet frame to do the direction back and forth movement perpendicular to described test tube rack plane of rotation respectively.
2. eccentric swing according to claim 1 nucleic acid extraction instrument it is characterised in that: described test tube rack drive mechanism Including the motor being arranged on pedestal, by described motor-driven eccentric stiffener, the output of the eccentric shaft on described eccentric stiffener End connects described test tube rack, drives described test tube rack to do eccentric rotary.
3. eccentric swing according to claim 2 nucleic acid extraction instrument it is characterised in that: described eccentric stiffener includes setting Put the output wheel on motor output shaft, the driving wheel being driven by output wheel, described driving wheel drives eccentric shaft turns.
4. eccentric swing according to claim 3 nucleic acid extraction instrument it is characterised in that:
Described output wheel is gear, drives described driving wheel by change-speed gearing group;
Or described output wheel is belt wheel, drives described driving wheel by conveyer belt.
5. eccentric swing according to claim 4 nucleic acid extraction instrument it is characterised in that: described driving wheel is provided with flat Weighing apparatus block, for keeping described driving wheel smooth rotation.
6. eccentric swing according to claim 5 nucleic acid extraction instrument it is characterised in that: described eccentric shaft include drive Portion, positioned at lower fixed part and the upper drive division of drive division both sides, described lower fixed part is fixed on described pedestal by bearing, institute State drive division and one dynamic gripper shoe is connected by bearing, described dynamic gripper shoe arranges described test tube rack.
7. eccentric swing according to claim 6 nucleic acid extraction instrument it is characterised in that: described dynamic gripper shoe bottom sets Put a retainer ring, described upper drive division is spacing, and bearing on described upper drive division is a clutch shaft bearing in described retainer ring, Bearing on described lower fixed part is two spaced bearings, respectively second bearing and 3rd bearing.
8. eccentric swing according to claim 7 nucleic acid extraction instrument it is characterised in that: arrange some on described pedestal Fixing pillar, described fixing pillar is distributed in encirclement mode, described fixing pillar arranges outer polarization ring, in described outer polarization ring Polarization ring in setting, described interior polarization ring is fixed in described retainer ring by connecting plate, and described interior polarization ring passes through the first bullet Reed is fixing to be polarized on ring outside, and described outer polarization ring is fixed on described fixing pillar by second spring piece.
9. the eccentric swing according to any one in claim 1-8 nucleic acid extraction instrument it is characterised in that: also include Electronic control system, described electronic control system is connected with described motor, bar magnet drive mechanism and bar magnet set drive mechanism signal, For controlling described test tube rack, magnetosheath frame and bar magnet frame action respectively.
10. the eccentric swing according to any one in claim 1-8 nucleic acid extraction instrument it is characterised in that: also wrap Include attemperating unit, for invisible spectro temperature of liquid in temperature control and/or test tubes frame.
CN201620919936.9U 2016-08-22 2016-08-22 Eccentric wobbling nucleic acid extraction equipment Active CN205893243U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106318847A (en) * 2016-08-22 2017-01-11 杭州奥盛仪器有限公司 Nucleic acid extraction equipment capable of eccentric oscillation
CN109266547A (en) * 2018-11-27 2019-01-25 嘉兴领宇科技有限公司 Nucleic acid-extracting apparatus

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106318847A (en) * 2016-08-22 2017-01-11 杭州奥盛仪器有限公司 Nucleic acid extraction equipment capable of eccentric oscillation
CN109266547A (en) * 2018-11-27 2019-01-25 嘉兴领宇科技有限公司 Nucleic acid-extracting apparatus

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