CN103969459B - A kind of full-automatic biochemical analysis method - Google Patents
A kind of full-automatic biochemical analysis method Download PDFInfo
- Publication number
- CN103969459B CN103969459B CN201410175562.XA CN201410175562A CN103969459B CN 103969459 B CN103969459 B CN 103969459B CN 201410175562 A CN201410175562 A CN 201410175562A CN 103969459 B CN103969459 B CN 103969459B
- Authority
- CN
- China
- Prior art keywords
- sample
- reagent
- reaction
- application
- stirring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a kind of full-automatic biochemical analysis method, comprise the following steps: 1) preparation process; 2) sampling, application of sample and whipping step: the reaction cup reaction tray transferred to by sample by control circuit module drive XYZ three-axis moving system drive reagent-sample application and stirring system from sample storehouse, and reagent transferred to the reaction cup reaction tray from agent bin; After reagent and sample have shifted, by reagent-sample application and stirring system, the liquid in reaction cup is stirred; The concrete liquid measure of sampling and application of sample is controlled by ram pump; 3) biochemical reaction and detecting step; 4) cleaning step; Full-automatic biochemical analysis method of the present invention is integrated with the whole process robotization of the operations such as sampling, application of sample, stirring, analysis detection, cleaning, and automaticity is high, easy to operate, and the equipment cost related to is low, and testing efficiency is high, is easy to promotion and implementation.
Description
Technical field
The present invention relates to a kind of full-automatic biochemical analysis method.
Background technology
At present, automatic clinical chemistry analyzer device is as one of important instrument often used in clinical examination, generally be used in each big-and-middle-sized hospital, give clinically to the diagnosis of disease, treatment and prognosis and health status provide requisite information foundation, become the standard configuration instrument that big-and-middle-sized hospital is indispensable, but it is large also to there is volume in existing automatic clinical chemistry analyzer, complex structure, price is high, safeguard the shortcomings such as inconvenience, an automatic clinical chemistry analyzer often needs hundreds of thousands just can purchase, small hospital, small towns health in one's power She Kang center etc. usually ability do not purchase, which limits the comprehensively universal of automatic clinical chemistry analyzer, the difficulty to patient diagnosis and treatment is brought to the medical worker of vast basic unit,
Existing Biochemical Analyzer structure is very complicated, and such as sample adding system and stirring system are separate configuration, and the volume-diminished for analyser brings great obstacle; Therefore, be necessary to adopt unique design stirring and sample adding system to be integrated;
In addition, purging system complex structure, cost is high, therefore, needs the purging system easy to implement designing a kind of novel low cost to complete matting;
And existing Biochemical Analyzer, automaticity is not high, needs operating personnel to carry out manual application of sample, easily makes a fault, affect measuring accuracy and efficiency.
In sum, be necessary to design a kind of low cost, easy to implement, and the full-automatic biochemical analysis method that in biochemical analysis process, automaticity is high, not high to requirements of installation space.
Summary of the invention
Technical matters to be solved by this invention is to provide a kind of full-automatic biochemical analysis method, and this full-automatic biochemical analysis method has low cost, easy to implement, and in biochemical analysis process, automaticity is high, not high to requirements of installation space.
The technical solution of invention is as follows:
A kind of new automatic biochemical analysis method, adopt new automatic Biochemical Analyzer, described new automatic Biochemical Analyzer comprises base (15), reagent-sample application and stirring system (4) and the control circuit module arranged on base, sample storing unit, reagent storage device, revolving reaction tray (13), the stepper motor rotated for driving reaction tray, for the purging system (11) of cleaning reaction dish, for accurately sampling and the ram pump (12) of application of sample, for the photodetector system (9) that detects liquid in reaction tray and for driving the XYZ three-axis moving system of described reagent-sample application and stirring system motion,
Described reaction tray (13), stepper motor, purging system (11), ram pump (12) and photodetector system (9) are all controlled by control circuit module;
Sample storing unit comprises sample rack (8) and is arranged on the sample storehouse (7) on sample rack;
Reagent storage device comprises reagent rack (6) and is arranged on the agent bin (5) in reagent rack; Bottom agent bin, cooling piece is installed; [have another name called Peltier, semiconductor chilling plate, thermoelectric semiconductor refrigerating assembly etc., in the cabin being provided for agent bin, working temperature remains on 2 ~ 15 DEG C]
Reaction tray adopts cylindrical structural, and the top of reaction tray is circumferentially evenly provided with multiple reaction cup; Heating collar [temperature constant state of reaction tray can be kept when instrument runs] is provided with bottom reaction tray;
Described XYZ three-axis moving system comprises the X that is connected successively by slide rail-slide block mechanism to slide assemblies, Y-direction slide assemblies and Z-direction slide assemblies; Described reagent-sample application and stirring system (4) are arranged on Z-direction slide assemblies;
Described reagent-sample application and stirring system comprise holder (60), stirring motor (51), driving wheel (52), eccentric wheel (54) and specimen needle (55); Stirring motor (51) is fixed on holder (60); Eccentric wheel (54) is arranged on holder (60) by clutch shaft bearing (61); Specimen needle (55) is arranged in the eccentric orfice of eccentric wheel (60) by the second bearing (56); Stirring motor (51) drives eccentric wheel (54) to rotate by driving wheel (52); Driving wheel (52) and eccentric wheel (54) are in transmission connection by belt (53); Stirring motor drives eccentric wheel to rotate by driving wheel; Eccentric wheel drives specimen needle to produce stirring action, and specimen needle is stirred the liquid in container while application of sample or sampling.
The process that described full-automatic biochemical is analyzed is as follows:
1) preparation process:
Store respectively in sample storehouse and agent bin and carry out sample needed for reaction test and reagent;
2) sampling, application of sample and whipping step:
The reaction cup reaction tray transferred to by sample by control circuit module drive XYZ three-axis moving system drive reagent-sample application and stirring system from sample storehouse, and reagent transferred to the reaction cup reaction tray from agent bin; After reagent and sample have shifted, by reagent-sample application and stirring system, the liquid in reaction cup is stirred; The concrete liquid measure of sampling and application of sample is controlled by ram pump;
3) biochemical reaction and detecting step;
Reagent and sample carry out biochemical reaction in the reaction cup of reaction tray; In course of reaction, the temperature constant state that the heating collar bottom reaction tray makes the liquid-retentive in reaction cup preset; After having reacted, by photodetector system, biochemistry detection and analysis are implemented to reacted liquid, obtain and detect and analysis result;
4) cleaning step;
After having reacted, control circuit module controls purging system and cleans reaction cup; In order to next round biochemistry detection and analytic process;
In above step, if relate to more than 2 reaction cup operations, then rotated by step motor control reaction tray by control circuit module, make reagent-sample application and stirring system, photodetector system and purging system apply operation to different reaction cup.
Described holder is made up of with the one piece of vertical plate docking being connected these 2 blocks of leveling boards upper and lower 2 blocks of leveling boards, the longitudinal section of holder is "] " type, form a depressed part between 2 blocks of leveling boards and one piece of vertical plate, described stirring motor is installed in this depressed part;
Two leveling boards of holder are respectively provided with one for installing the through hole of specimen needle; The dead in line of 2 through holes and be vertical direction, the through hole being positioned at below is provided with described clutch shaft bearing and eccentric wheel; The top of specimen needle is stretched out from the through hole being positioned at top.
Reagent-sample application and stirring system also comprise anticollision mechanism, described anticollision mechanism comprises the pcb board (59) of the upper crashproof optocoupler of band (57) arranged of holder (60), and the light barrier (58) arranged on the top of specimen needle (55); The stage clip for resetting is provided with in the middle part of specimen needle and between superposed leveling board; When specimen needle is clashed into and risen, drive light barrier to rise the light path blocked in crashproof optocoupler between transmitting terminal and receiving end, the output signal of crashproof optocoupler is changed, namely triggers crashproof optocoupler and send alerting signal; After the control circuit module (as microprocessor) of full automatic biochemical apparatus receives this alerting signal, drive specimen needle rollback to avoid occurring again to collide.
The axis 0.1mm of the axis runout eccentric wheel (54) of eccentric orfice.
Described purging system comprises straight-line motion mechanism and cleaning needle, and described straight-line motion mechanism comprises drive motor (21), base plate (31), driving wheel (24), engaged wheel (25), portable plate (29) and vertical plate (22);
Cleaning needle is arranged on portable plate;
Drive motor is arranged on base plate or is arranged on the lower end of vertical plate; The output shaft of drive motor connects driving wheel; Engaged wheel is arranged on the upper end of vertical plate; The axis of driving wheel and engaged wheel is static relative to vertical plate; Driving wheel and engaged wheel are in transmission connection by Timing Belt (26);
2 sides of vertical plate are slide rail (23); The both sides of portable plate are provided with the pulley (30) with described slide rail adaptation; Portable plate is positioned at the front of vertical plate, and driving wheel, engaged wheel and Timing Belt are all located at the back side of vertical plate; The bar shaped pilot hole (41) of vertical direction is had in the middle part of vertical plate; Gangbar (27) fixing on portable plate is stretched out from this bar shaped pilot hole and is fixed on Timing Belt.[portable plate is driven to slide up and down along slide rail by Timing Belt.】
Hall sensing plate (23) is fixed with at the top of vertical plate; Magnet (40) is fixed with at the top of portable plate.[for the position of sensed activity plate opposed vertical plate, avoiding specimen needle and miscellaneous part to clash into]
Portable plate is provided with an eccentric nut (39), and pulley (30) is arranged on eccentric nut (39) by screw (34).[by the U-type groove of eccentric nut adjustable pulley and the gap of slide rail, thus coordinating tightness between adjustable pulley with slide rail]
Reagent rack (6) and sample rack (8) that reagent and sample are housed are shifted onto in agent bin and sample storehouse by the guide rail on agent bin and sample storehouse, reaction tray (13) is arranged in reaction base plate (14), reaction tray is driven to rotate by stepper motor, reagent-sample application and stirring system (4) by X to slide assemblies (10), Y-direction slide assemblies (2), the move mode of Z-direction slide assemblies (3) three-shaft linkage carries out collection and the application of sample of reagent and sample, and be integrated with the function of application of sample and stirring, its sample needle is directly possessed stir function, the accurate application of sample of reagent and sample is realized by ram pump (12), the measuring ability of sample is completed by photodetector system (9).
Purging system (11) realizes moving up and down of its cleaning needle by U-type groove bearing fixed thereon and the cooperation of slide bar and the drive of stepper motor.
In photodetector system (9), the rotation of filter wheel is driven to realize the switching of optical filter by special stepper motor.
Reaction tray component bottom has heating collar, the temperature constant state of reaction tray can be kept when instrument runs, cuvette (i.e. reaction cup) in reaction tray is cleaned by purging system (11) after test completes, reagent-sample application and stirring system (4) by X to slide assemblies (10), Y-direction slide assemblies (2), the move mode of Z-direction slide assemblies (3) three-shaft linkage carries out collection and the application of sample of reagent and sample, and be integrated with the function of application of sample and stirring, its sample needle is directly possessed stir function, thus eliminate a set of reagent-sample stirring system, the accurate application of sample of reagent and sample is realized by ram pump (12).Ram pump is a kind of device of energy liquid with precise control application of sample amount, it is the plunger connected by the screw rod on stepper motor in ram pump, just can accurately control the upper and lower of plunger by the rotation of control step motor, thus the turnover of liquid with precise control [ram pump is prior art] realizes the measuring ability of sample by photodetector system (9).
It is spectroscopic modes before using in described photodetector system (9), by driven by motor synchronous pulley, driving filter wheel rotates, the optical filter of 8 groups of different wave lengths filter wheel is equipped with, realize the demand of different reagent to different wave length, according to bright rich Beer law, use opto-electronic conversion mechanism to detect by the monochromatic light after cuvette, and be translated into electric signal.
Reaction tray is installed ringwise multiple (as 48) reaction cup, multiple reagent bottle (having another name called agent bin) (as 50) is placed with in reagent rack (6), agent bin slips into reagent rack by the guide rail be arranged in reagent rack, multiple sample cup or heparin tube (i.e. sample storehouse) (as 22) is placed with in sample rack (8), sample storehouse slips into sample rack by the guide rail be arranged on sample rack, is provided with a Peltier and [has another name called under agent bin
cooling piece, semiconductor chilling plate, thermoelectric semiconductor refrigerating assembly etc.] make working temperature in the cabin of agent bin remain on 2 ~ 15 DEG C, reaction tray (13) is arranged in reaction base plate (14), Timing Belt is driven by stepper motor, Timing Belt drives the synchronous pulley on reaction tray assembly to rotate, freely rotating of reaction tray on realization response dish assembly, reaction tray component bottom has heating collar, the temperature constant state of reaction tray can be kept when instrument runs, reagent-sample application and stirring system (4) have application of sample and the agitating function of reagent-sample simultaneously, directly can extend in reaction cup after the application of sample of sample completes and carry out stirring action, thus eliminate a set of independent reagent-sample stirring system, reagent-sample application and stirring system (4) are arranged on the slide rail of Z-direction slide assemblies (3), stepper motor by Z-direction slide assemblies drives reagent-sample application and stirring system to do upper and lower rectilinear motion, Z-direction slide assemblies is arranged on Y-direction slide assemblies, stepper motor by Y-direction slide assemblies drives Z-direction slide assemblies and the reagent-sample application be fixed on Z-direction slide assemblies and stirring system to do tandem motion, and Y-direction slide assemblies (2) and be above-mentionedly attached to entirety on Y-direction slide assemblies and be together arranged on X on slide assemblies (10), its left and right rectilinear motion is realized to the stepper motor on slide assemblies by X, reagent-sample application and stirring system (4) are connected on ram pump (12) by pipeline, the application of sample amount of reagent-sample application and stirring system (4) accurately can be controlled by ram pump (12), photodetector system (9) is by rotating by the filter wheel of step motor control the switching realizing different wave length optical filter, by the Halogen lamp LED be fixed therein and the measuring ability being fixed on the photoelectric conversion device realization response value in reaction tray (13) assembly, purging system (11) realizes moving up and down of its cleaning needle by U-type groove bearing fixed thereon and the cooperation of slide bar and the drive of stepper motor, by being connected to the cleaning function of the fluid path part realization response cup on cleaning needle.
Beneficial effect:
Full-automatic biochemical analysis method of the present invention, avoid and adopt conventional automatic clinical chemistry analyzer to take up room larger, complex structure, the shortcoming that cost is high, the Biochemical Analyzer adopted achieves miniaturization, the lightness of automatic clinical chemistry analyzer, greatly reduce the requirement of installing space, convenient transport and installation, and automaticity is high.
In the Biochemical Analyzer that full-automatic biochemical analysis method adopts, reagent-sample application and stirring system and purging system are of the present invention 2 large outstanding features:
(1) reagent-sample application and stirring system (i.e. integrated sample pin application of sample and stirring system) are shaken by specimen needle self High Rotation Speed dexterously and are stirred reagent and sample, make specimen needle one pin multiplex like this, structure is simpler and make biochemical analyzer operational efficiency higher.Specifically, be adopt driven by motor specimen needle High Rotation Speed to make the syringe needle of specimen needle rock to stir sample and reagent, this novel rabbling mechanism makes equipment mechanism simple and stable more; Make the novel rabbling mechanism of specimen needle and specimen needle anticollision mechanism integrate simultaneously, make specimen needle can realize high-speed stirred function simultaneously; Integrated sample pin application of sample and stirring system also have collision prevention function; namely specimen needle can be protected to exempt from shock; after specimen needle is impacted; specimen needle is raised; the change in location of optocoupler induction light barrier; there is provided electric signal to report to the police to the main control module of equipment (i.e. automatic clinical chemistry analyzer device), then this main control module according to the timely rollback of this alerting signal this integrated sample pin application of sample and stirring system, thus can play the effect of protection specimen needle.Optocoupler is made up of two parts, transmitting terminal and receiving end, has bar gap between two parts, when light barrier rises, is just being sandwiched in this gap the light path blocked between transmitting terminal and receiving end.Transmitting terminal continues to send light signal, and when the light path of optocoupler is not by stop, receiving end can receive the signal that transmitting terminal sends, once light barrier rises block light path, then receiving end cannot receive signal, then send alerting signal.Because specimen needle can inject sample and reagent, also sample and reagent can be stirred evenly, and prevent specimen needle from collision, therefore, this mechanism gathers multi-function in integral whole, make automatic clinical chemistry analyzer device structure more compact small and exquisite, improve the work efficiency of equipment simultaneously, use also convenient.Therefore, this integrated sample pin application of sample and stir system design simple and ingenious, have application of sample, stirring and collision prevention function concurrently, can remarkable simplified apparatus structure, easy to implement, easy to use, can effectively improve testing and analysis efficiency.
(2) core of wiper mechanism is improved and is to adopt novel straight-line motion mechanism, and structure is simple, and stable and smooth operation, significantly can reduce straight-line motion mechanism manufacturing cost and manufacturing accuracy requirement.This straight-line motion mechanism adopts the contact movement of 3 pulleys 30 and slide rail 3 to realize rectilinear motion, 3 pulleys 30 are pushed down slide rail (23) from the both sides of slide rail 23 respectively, portable plate (29) is made to move linearly up and down on slide rail (23) and to realize guide function, the pulley (30) of side regulates the dynamics and gap realization adjustment and fixed function that compress by eccentric nut (39), this straight-line motion mechanism makes equipment mechanism simple and stable more, stable and smooth operation, significantly can reduce straight-line motion mechanism manufacturing cost and manufacturing accuracy requirement simultaneously.Be connected with Timing Belt (26) and strain between driving wheel (24) with engaged wheel (25), gangbar is fixed on Timing Belt, the relative position of driving wheel and engaged wheel is constant, such portable plate (29) just can pass through drive motor (21) and drive Timing Belt (26) to realize the driving of rectilinear motion, this simple and compact for structure, require low to installation and manufacturing accuracy.The top of vertical plate (22) is provided with hall sensing plate (33), crashproof and positioning function is realized by the position of the upper magnet (40) installed of hall sensing plate (33) sensed activity plate (29), this structure farthest simplifies mechanism, and realizes crashproof positioning function and the operability of straight-line motion mechanism by this mechanism.Hall sensing plate (33) is provided with gland in order to protect hall sensing plate (33).Thus, this Novel linear motion machine structure novel structure and simple, not high to accuracy requirement, can flexible pulley and the tightness that coordinates between slide rail, and there is crashproof and positioning function, thus feature richness.
In sum, full-automatic biochemical analysis method of the present invention have employed novel integrated sample pin application of sample and stirring system, greatly reduce the space of whole analyser, huge contribution has been made in miniaturization for instrument, in addition, adopts novel straight-line motion mechanism (being under the jurisdiction of purging system) to realize moving up and down of cleaning needle, structure is simple, easy to implement, cost is low, reduces the cost of analyser significantly; Simultaneously, by the structure of reaction tray, be integrated with multiple reaction cup, coordinate the switching of the rotation realization response cup of driving stepper motor reaction tray, then realized sampling and the application of sample of specimen needle by XYZ three-axis moving system, whole system structure is very compact, run smooth, adopt sample storehouse and the agent bin of slide rail type, easy to operate, can multiple sample and plurality of reagents be held, for analytical test brings great convenience.Therefore, full-automatic biochemical analysis method of the present invention is integrated with the whole process robotization of the operations such as sampling, application of sample, stirring, analysis detection, cleaning, and automaticity is high, easy to operate, and the equipment cost related to is low, and testing efficiency is high, is easy to promotion and implementation.
Accompanying drawing explanation
Fig. 1 is the general structure schematic diagram of new automatic Biochemical Analyzer;
Fig. 2 is the stereographic map of straight-line motion mechanism.
Fig. 3 is the stereographic map at another visual angle of straight-line motion mechanism;
Fig. 4 is the general structure schematic diagram (stereographic map) of integral type specimen needle application of sample and stirring system;
Fig. 5 is the general structure schematic diagram (front elevation) of integral type specimen needle application of sample and stirring system;
Fig. 6 is that A-A in Fig. 5 is to sectional view.
Label declaration: 1-protective cover plate, 2-Y is to slide assemblies, and 3-Z is to slide assemblies, 4-reagent-sample application and stirring system, 5-agent bin, 6-reagent rack, 7-sample storehouse, 8-sample rack, 9-photodetector system, 10-X is to slide assemblies, 11-purging system, 12-ram pump, 13-reaction tray, 14-reacts base plate, 15-base.
21-drive motor, 22-vertical plate, 23-slide rail, 24-driving wheel, 25-engaged wheel, 26-Timing Belt, 27-gangbar, 29-portable plate, 30-pulley, 31-base plate, 33-hall sensing plate, 34-screw, 39-eccentric nut, 40-magnet, 41-bar shaped pilot hole.
51-stirring motor, 52-driving wheel, 53-belt, 54-eccentric wheel, 55-specimen needle, 56-second bearing, the crashproof optocoupler of 57-, 58-light barrier, 59-PCB plate, 60-holder, 61-clutch shaft bearing.
Embodiment
Below with reference to the drawings and specific embodiments, the present invention is described in further details:
Embodiment 1:
As Fig. 1-6, a kind of new automatic biochemical analysis method, adopt new automatic Biochemical Analyzer, described new automatic Biochemical Analyzer comprises base (15), reagent-sample application and stirring system (4) and the control circuit module arranged on base, sample storing unit, reagent storage device, revolving reaction tray (13), the stepper motor rotated for driving reaction tray, for the purging system (11) of cleaning reaction dish, for accurately sampling and the ram pump (12) of application of sample, for the photodetector system (9) that detects liquid in reaction tray and for driving the XYZ three-axis moving system of described reagent-sample application and stirring system motion,
Described reaction tray (13), stepper motor, purging system (11), ram pump (12) and photodetector system (9) are all controlled by control circuit module;
Sample storing unit comprises sample rack (8) and is arranged on the sample storehouse (7) on sample rack;
Reagent storage device comprises reagent rack (6) and is arranged on the agent bin (5) in reagent rack; Bottom agent bin, cooling piece is installed; [have another name called Peltier, semiconductor chilling plate, thermoelectric semiconductor refrigerating assembly etc., in the cabin being provided for agent bin, working temperature remains on 2 ~ 15 DEG C]
Reaction tray adopts cylindrical structural, and the top of reaction tray is circumferentially evenly provided with multiple reaction cup; Heating collar [temperature constant state of reaction tray can be kept when instrument runs] is provided with bottom reaction tray;
Described XYZ three-axis moving system comprises the X that is connected successively by slide rail-slide block mechanism to slide assemblies, Y-direction slide assemblies and Z-direction slide assemblies; Described reagent-sample application and stirring system (4) are arranged on Z-direction slide assemblies;
Described reagent-sample application and stirring system comprise holder (60), stirring motor (51), driving wheel (52), eccentric wheel (54) and specimen needle (55); Stirring motor (51) is fixed on holder (60); Eccentric wheel (54) is arranged on holder (60) by clutch shaft bearing (61); Specimen needle (55) is arranged in the eccentric orfice of eccentric wheel (60) by the second bearing (56); Stirring motor (51) drives eccentric wheel (54) to rotate by driving wheel (52); Driving wheel (52) and eccentric wheel (54) are in transmission connection by belt (53); Stirring motor drives eccentric wheel to rotate by driving wheel; Eccentric wheel drives specimen needle to produce stirring action, and specimen needle is stirred the liquid in container while application of sample or sampling.
The process that described full-automatic biochemical is analyzed is as follows:
1) preparation process:
Store respectively in sample storehouse and agent bin and carry out sample needed for reaction test and reagent;
2) sampling, application of sample and whipping step:
The reaction cup reaction tray transferred to by sample by control circuit module drive XYZ three-axis moving system drive reagent-sample application and stirring system from sample storehouse, and reagent transferred to the reaction cup reaction tray from agent bin; After reagent and sample have shifted, by reagent-sample application and stirring system, the liquid in reaction cup is stirred; The concrete liquid measure of sampling and application of sample is controlled by ram pump;
3) biochemical reaction and detecting step;
Reagent and sample carry out biochemical reaction in the reaction cup of reaction tray; In course of reaction, the temperature constant state that the heating collar bottom reaction tray makes the liquid-retentive in reaction cup preset; After having reacted, by photodetector system, biochemistry detection and analysis are implemented to reacted liquid, obtain and detect and analysis result;
4) cleaning step;
After having reacted, control circuit module controls purging system and cleans reaction cup; In order to next round biochemistry detection and analytic process;
In above step, if relate to more than 2 reaction cup operations, then rotated by step motor control reaction tray by control circuit module, make reagent-sample application and stirring system, photodetector system and purging system apply operation to different reaction cup.
Described holder is made up of with the one piece of vertical plate docking being connected these 2 blocks of leveling boards upper and lower 2 blocks of leveling boards, the longitudinal section of holder is "] " type, form a depressed part between 2 blocks of leveling boards and one piece of vertical plate, described stirring motor is installed in this depressed part;
Two leveling boards of holder are respectively provided with one for installing the through hole of specimen needle; The dead in line of 2 through holes and be vertical direction, the through hole being positioned at below is provided with described clutch shaft bearing and eccentric wheel; The top of specimen needle is stretched out from the through hole being positioned at top.
Reagent-sample application and stirring system also comprise anticollision mechanism, described anticollision mechanism comprises the pcb board (59) of the upper crashproof optocoupler of band (57) arranged of holder (60), and the light barrier (58) arranged on the top of specimen needle (55); The stage clip for resetting is provided with in the middle part of specimen needle and between superposed leveling board; When specimen needle is clashed into and risen, drive light barrier to rise the light path blocked in crashproof optocoupler between transmitting terminal and receiving end, the output signal of crashproof optocoupler is changed, namely triggers crashproof optocoupler and send alerting signal; After the control circuit module (as microprocessor) of full automatic biochemical apparatus receives this alerting signal, drive specimen needle rollback to avoid occurring again to collide.
The axis 0.1mm of the axis runout eccentric wheel (54) of eccentric orfice.
Described purging system comprises straight-line motion mechanism and cleaning needle, and described straight-line motion mechanism comprises drive motor (21), base plate (31), driving wheel (24), engaged wheel (25), portable plate (29) and vertical plate (22);
Cleaning needle is arranged on portable plate;
Drive motor is arranged on base plate or is arranged on the lower end of vertical plate; The output shaft of drive motor connects driving wheel; Engaged wheel is arranged on the upper end of vertical plate; The axis of driving wheel and engaged wheel is static relative to vertical plate; Driving wheel and engaged wheel are in transmission connection by Timing Belt (26);
2 sides of vertical plate are slide rail (23); The both sides of portable plate are provided with the pulley (30) with described slide rail adaptation; Portable plate is positioned at the front of vertical plate, and driving wheel, engaged wheel and Timing Belt are all located at the back side of vertical plate; The bar shaped pilot hole (41) of vertical direction is had in the middle part of vertical plate; Gangbar (27) fixing on portable plate is stretched out from this bar shaped pilot hole and is fixed on Timing Belt.[portable plate is driven to slide up and down along slide rail by Timing Belt.】
Hall sensing plate (23) is fixed with at the top of vertical plate; Magnet (40) is fixed with at the top of portable plate.[for the position of sensed activity plate opposed vertical plate, avoiding specimen needle and miscellaneous part to clash into]
Portable plate is provided with an eccentric nut (39), and pulley (30) is arranged on eccentric nut (39) by screw (34).[by the U-type groove of eccentric nut adjustable pulley and the gap of slide rail, thus coordinating tightness between adjustable pulley with slide rail]
Reagent rack (6) and sample rack (8) that reagent and sample are housed are shifted onto in agent bin and sample storehouse by the guide rail on agent bin and sample storehouse, reaction tray (13) is arranged in reaction base plate (14), reaction tray is driven to rotate by stepper motor, reagent-sample application and stirring system (4) by X to slide assemblies (10), Y-direction slide assemblies (2), the move mode of Z-direction slide assemblies (3) three-shaft linkage carries out collection and the application of sample of reagent and sample, and be integrated with the function of application of sample and stirring, its sample needle is directly possessed stir function, the accurate application of sample of reagent and sample is realized by ram pump (12), the measuring ability of sample is completed by photodetector system (9).
Purging system (11) realizes moving up and down of its cleaning needle by U-type groove bearing fixed thereon and the cooperation of slide bar and the drive of stepper motor.
In photodetector system (9), the rotation of filter wheel is driven to realize the switching of optical filter by special stepper motor.
Reaction tray component bottom has heating collar, the temperature constant state of reaction tray can be kept when instrument runs, cuvette (i.e. reaction cup) in reaction tray is cleaned by purging system (11) after test completes, reagent-sample application and stirring system (4) by X to slide assemblies (10), Y-direction slide assemblies (2), the move mode of Z-direction slide assemblies (3) three-shaft linkage carries out collection and the application of sample of reagent and sample, and be integrated with the function of application of sample and stirring, its sample needle is directly possessed stir function, thus eliminate a set of reagent-sample stirring system, the accurate application of sample of reagent and sample is realized by ram pump (12).Ram pump is a kind of device of energy liquid with precise control application of sample amount, it is the plunger connected by the screw rod on stepper motor in ram pump, just can accurately control the upper and lower of plunger by the rotation of control step motor, thus the turnover of liquid with precise control [ram pump is prior art] realizes the measuring ability of sample by photodetector system (9).
It is spectroscopic modes before using in described photodetector system (9), by driven by motor synchronous pulley, driving filter wheel rotates, the optical filter of 8 groups of different wave lengths filter wheel is equipped with, realize the demand of different reagent to different wave length, according to bright rich Beer law, use opto-electronic conversion mechanism to detect by the monochromatic light after cuvette, and be translated into electric signal.
Reaction tray is installed ringwise multiple (as 48) reaction cup, multiple reagent bottle (having another name called agent bin) (as 50) is placed with in reagent rack (6), agent bin slips into reagent rack by the guide rail be arranged in reagent rack, multiple sample cup or heparin tube (i.e. sample storehouse) (as 22) is placed with in sample rack (8), sample storehouse slips into sample rack by the guide rail be arranged on sample rack, is provided with a Peltier and [has another name called under agent bin
cooling piece, semiconductor chilling plate, thermoelectric semiconductor refrigerating assembly etc.] make working temperature in the cabin of agent bin remain on 2 ~ 15 DEG C, reaction tray (13) is arranged in reaction base plate (14), Timing Belt is driven by stepper motor, Timing Belt drives the synchronous pulley on reaction tray assembly to rotate, freely rotating of reaction tray on realization response dish assembly, reaction tray component bottom has heating collar, the temperature constant state of reaction tray can be kept when instrument runs, reagent-sample application and stirring system (4) have application of sample and the agitating function of reagent-sample simultaneously, directly can extend in reaction cup after the application of sample of sample completes and carry out stirring action, thus eliminate a set of independent reagent-sample stirring system, reagent-sample application and stirring system (4) are arranged on the slide rail of Z-direction slide assemblies (3), stepper motor by Z-direction slide assemblies drives reagent-sample application and stirring system to do upper and lower rectilinear motion, Z-direction slide assemblies is arranged on Y-direction slide assemblies, stepper motor by Y-direction slide assemblies drives Z-direction slide assemblies and the reagent-sample application be fixed on Z-direction slide assemblies and stirring system to do tandem motion, and Y-direction slide assemblies (2) and be above-mentionedly attached to entirety on Y-direction slide assemblies and be together arranged on X on slide assemblies (10), its left and right rectilinear motion is realized to the stepper motor on slide assemblies by X, reagent-sample application and stirring system (4) are connected on ram pump (12) by pipeline, the application of sample amount of reagent-sample application and stirring system (4) accurately can be controlled by ram pump (12), photodetector system (9) is by rotating by the filter wheel of step motor control the switching realizing different wave length optical filter, by the Halogen lamp LED be fixed therein and the measuring ability being fixed on the photoelectric conversion device realization response value in reaction tray (13) assembly, purging system (11) realizes moving up and down of its cleaning needle by U-type groove bearing fixed thereon and the cooperation of slide bar and the drive of stepper motor, by being connected to the cleaning function of the fluid path part realization response cup on cleaning needle.
Be understandable that; for the person of ordinary skill of the art; can make other various corresponding change and distortion according to technical scheme of the present invention and technical conceive, and all these change and be out of shape the protection domain that all should belong to the accompanying claim of the present invention.
Claims (7)
1. a new automatic biochemical analysis method, it is characterized in that, adopt new automatic Biochemical Analyzer, described new automatic Biochemical Analyzer comprises base (15), reagent-sample application and stirring system (4) and the control circuit module arranged on base, sample storing unit, reagent storage device, revolving reaction tray (13), the stepper motor rotated for driving reaction tray, for the purging system (11) of cleaning reaction dish, for accurately sampling and the ram pump (12) of application of sample, for the photodetector system (9) that detects liquid in reaction tray and for driving the XYZ three-axis moving system of described reagent-sample application and stirring system motion,
Described reaction tray (13), stepper motor, purging system (11), ram pump (12) and photodetector system (9) are all controlled by control circuit module;
Sample storing unit comprises sample rack (8) and is arranged on the sample storehouse (7) on sample rack;
Reagent storage device comprises reagent rack (6) and is arranged on the agent bin (5) in reagent rack; Bottom agent bin, cooling piece is installed;
Reaction tray adopts cylindrical structural, and the top of reaction tray is circumferentially evenly provided with multiple reaction cup; Heating collar is provided with bottom reaction tray;
Described XYZ three-axis moving system comprise the X that is connected successively by slide rail-slide block mechanism to slide assemblies,
Y-direction slide assemblies and Z-direction slide assemblies; Described reagent-sample application and stirring system (4) are arranged
On Z-direction slide assemblies;
Described reagent-sample application and stirring system comprise holder (60), stirring motor (51), driving wheel (52), eccentric wheel (54) and specimen needle (55); Stirring motor (51) is fixed on holder (60); Eccentric wheel (54) is arranged on holder (60) by clutch shaft bearing (61); Specimen needle (55) is arranged in the eccentric orfice of eccentric wheel (60) by the second bearing (56); Stirring motor (51) drives eccentric wheel (54) to rotate by driving wheel (52); Driving wheel (52) and eccentric wheel (54) are in transmission connection by belt (53); Stirring motor drives eccentric wheel to rotate by driving wheel; Eccentric wheel drives specimen needle to produce stirring action, and specimen needle is stirred the liquid in container while application of sample or sampling;
The process that described full-automatic biochemical is analyzed is as follows:
1) preparation process:
Store respectively in sample storehouse and agent bin and carry out sample needed for reaction test and reagent;
2) sampling, application of sample and whipping step:
The reaction cup reaction tray transferred to by sample by control circuit module drive XYZ three-axis moving system drive reagent-sample application and stirring system from sample storehouse, and reagent transferred to the reaction cup reaction tray from agent bin; After reagent and sample have shifted, by reagent-sample application and stirring system, the liquid in reaction cup is stirred; The concrete liquid measure of sampling and application of sample is controlled by ram pump;
3) biochemical reaction and detecting step;
Reagent and sample carry out biochemical reaction in the reaction cup of reaction tray; In course of reaction, the temperature constant state that the heating collar bottom reaction tray makes the liquid-retentive in reaction cup preset; After having reacted, by photodetector system, biochemistry detection and analysis are implemented to reacted liquid, obtain and detect and analysis result;
4) cleaning step;
After having reacted, control circuit module controls purging system and cleans reaction cup; In order to next round biochemistry detection and analytic process;
In above step, if relate to more than 2 reaction cup operations, then rotated by step motor control reaction tray by control circuit module, make reagent-sample application and stirring system, photodetector system and purging system apply operation to different reaction cup.
2. new automatic biochemical analysis method according to claim 1, it is characterized in that, described holder is made up of with the one piece of vertical plate docking being connected these 2 blocks of leveling boards upper and lower 2 blocks of leveling boards, the longitudinal section of holder is "] " type, form a depressed part between 2 blocks of leveling boards and one piece of vertical plate, described stirring motor is installed in this depressed part;
Two leveling boards of holder are respectively provided with one for installing the through hole of specimen needle; The dead in line of 2 through holes and be vertical direction, the through hole being positioned at below is provided with described clutch shaft bearing and eccentric wheel; The top of specimen needle is stretched out from the through hole being positioned at top.
3. new automatic biochemical analysis method according to claim 2, it is characterized in that, reagent-sample application and stirring system also comprise anticollision mechanism, described anticollision mechanism comprises the pcb board (59) of the upper crashproof optocoupler of band (57) arranged of holder (60), and the light barrier (58) arranged on the top of specimen needle (55); The stage clip for resetting is provided with in the middle part of specimen needle and between superposed leveling board; When specimen needle is clashed into and risen, drive light barrier to rise the light path blocked in crashproof optocoupler between transmitting terminal and receiving end, the output signal of crashproof optocoupler is changed, namely triggers crashproof optocoupler and send alerting signal; After the control circuit module of full automatic biochemical apparatus receives this alerting signal, drive specimen needle rollback to avoid occurring again to collide.
4. new automatic biochemical analysis method according to claim 3, is characterized in that, the axis 0.1mm of the axis runout eccentric wheel (54) of eccentric orfice.
5. the new automatic biochemical analysis method according to any one of claim 1-4, it is characterized in that, described purging system comprises straight-line motion mechanism and cleaning needle, and described straight-line motion mechanism comprises drive motor (21), base plate (31), driving wheel (24), engaged wheel (25), portable plate (29) and vertical plate (22);
Cleaning needle is arranged on portable plate;
Drive motor is arranged on base plate or is arranged on the lower end of vertical plate; The output shaft of drive motor connects driving wheel; Engaged wheel is arranged on the upper end of vertical plate; The axis of driving wheel and engaged wheel is static relative to vertical plate; Driving wheel and engaged wheel are in transmission connection by Timing Belt (26);
2 sides of vertical plate are slide rail (23); The both sides of portable plate are provided with the pulley (30) with described slide rail adaptation; Portable plate is positioned at the front of vertical plate, and driving wheel, engaged wheel and Timing Belt are all located at the back side of vertical plate; The bar shaped pilot hole (41) of vertical direction is had in the middle part of vertical plate; Gangbar (27) fixing on portable plate is stretched out from this bar shaped pilot hole and is fixed on Timing Belt.
6. new automatic biochemical analysis method according to claim 5, is characterized in that, is fixed with hall sensing plate (23) at the top of vertical plate; Magnet (40) is fixed with at the top of portable plate.
7. new automatic biochemical analysis method according to claim 6, it is characterized in that, portable plate is provided with an eccentric nut (39), and pulley (30) is arranged on eccentric nut (39) by screw (34).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410175562.XA CN103969459B (en) | 2014-04-22 | 2014-04-22 | A kind of full-automatic biochemical analysis method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410175562.XA CN103969459B (en) | 2014-04-22 | 2014-04-22 | A kind of full-automatic biochemical analysis method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103969459A CN103969459A (en) | 2014-08-06 |
| CN103969459B true CN103969459B (en) | 2015-08-19 |
Family
ID=51239207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410175562.XA Expired - Fee Related CN103969459B (en) | 2014-04-22 | 2014-04-22 | A kind of full-automatic biochemical analysis method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103969459B (en) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105897352B (en) * | 2016-05-30 | 2018-11-23 | 苏州赛腾精密电子股份有限公司 | A kind of wireless network card mechanism for testing |
| CN109187444A (en) * | 2016-08-25 | 2019-01-11 | 任文利 | A kind of haemoconcentration detection device |
| CN107014990A (en) * | 2017-05-15 | 2017-08-04 | 深圳麦科田生物医疗技术有限公司 | Full-automatic thrombus elastic force measuring device |
| CN107505471B (en) * | 2017-09-06 | 2023-11-28 | 中国科学院苏州生物医学工程技术研究所 | Thrombus elasticity testing device |
| CN108267607B (en) * | 2018-01-10 | 2021-06-29 | 济南星齐医学检验有限公司 | Automatic sample analysis and detection method |
| CN108177154A (en) * | 2018-01-26 | 2018-06-19 | 深圳迎凯生物科技有限公司 | Grab cup device |
| CN110275030B (en) * | 2018-03-13 | 2022-05-27 | 北京中生金域诊断技术股份有限公司 | Automatic analyzer for vaginal secretion |
| CN109061208A (en) * | 2018-08-02 | 2018-12-21 | 上海宝中盈仪器仪表有限公司 | Full-automatic biochemical oxygen demand detector |
| CN109633188B (en) * | 2018-12-28 | 2024-03-08 | 桂林优利特医疗电子有限公司 | Full-automatic multifunctional biochemical analyzer and application method thereof |
| WO2020142798A1 (en) * | 2019-01-11 | 2020-07-16 | Meon Medical Solutions Gmbh & Co Kg | Pipetting device and method for the transfer of fluids |
| CN109655421A (en) * | 2019-02-14 | 2019-04-19 | 杭州霆科生物科技有限公司 | A kind of full-automatic Detecting Pesticide system |
| CN110361553B (en) * | 2019-07-22 | 2023-05-23 | 迈克医疗电子有限公司 | Full-automatic sample analyzer and needle washing method thereof |
| CN112834500A (en) * | 2019-11-22 | 2021-05-25 | 穆鲁健 | POCT rapid detection analyzer |
| CN112881740A (en) * | 2019-11-29 | 2021-06-01 | 深圳市帝迈生物技术有限公司 | Sample adding assembly, sample analyzer and sample adding method |
| CN112129698A (en) * | 2020-10-30 | 2020-12-25 | 南京领跑生命健康产业技术研究院有限公司 | Biochemical analyzer system with modular design and working method thereof |
| CN113909067B (en) * | 2021-11-17 | 2023-11-24 | 北京大学人民医院 | Automatic coating medical device for increasing applicability of medical instrument |
| CN114377549B (en) * | 2022-01-18 | 2023-04-11 | 深圳汇芯生物医疗科技有限公司 | Automated separation apparatus and method for separating target particles in liquid sample |
| CN114509575B (en) * | 2022-04-19 | 2022-06-14 | 天津德祥生物技术有限公司 | Microfluidic detection device |
| CN115611965B (en) * | 2022-09-23 | 2024-02-09 | 江苏明生聚太生物科技有限公司 | Multichannel polypeptide synthesizer and synthesizing method thereof |
| CN115453137B (en) * | 2022-11-10 | 2023-03-24 | 长沙市鹏瑞生物科技有限公司 | Eccentric stirring assembly and sample needle sample adding and stirring system |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3051618B2 (en) * | 1993-10-25 | 2000-06-12 | 富士写真フイルム株式会社 | Dry analytical film supply device |
| JP3063584B2 (en) * | 1995-09-05 | 2000-07-12 | 株式会社日立製作所 | Automatic analyzer |
| JP3419430B2 (en) * | 1996-08-21 | 2003-06-23 | 日本電子株式会社 | Reagent injection mixer for biochemical automatic analyzer |
| US6824738B1 (en) * | 2000-04-14 | 2004-11-30 | Discovery Partners International, Inc. | System and method for treatment of samples on solid supports |
| JP3914837B2 (en) * | 2002-07-10 | 2007-05-16 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP4349893B2 (en) * | 2003-12-05 | 2009-10-21 | 株式会社日立ハイテクノロジーズ | Automatic analyzer and analysis method of automatic analyzer |
| JP2011149885A (en) * | 2010-01-25 | 2011-08-04 | Hitachi High-Technologies Corp | Automatic analyzer |
-
2014
- 2014-04-22 CN CN201410175562.XA patent/CN103969459B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN103969459A (en) | 2014-08-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103969459B (en) | A kind of full-automatic biochemical analysis method | |
| CN104034908B (en) | A kind of new automatic Biochemical Analyzer | |
| CN204269661U (en) | The sampling apparatus of full-automatic Hematometer | |
| CN2857015Y (en) | Sampling and stirring device of wholly automatic biochemistry amalyzer | |
| CN102147405B (en) | Fully automatic biochemical immune analyzer | |
| CN106018784A (en) | Small electrochemical luminescence immunoassay analyzer and analysis method thereof | |
| CN203337661U (en) | Full-automatic biochemistry and luminescence immunoassay system | |
| CN107014990A (en) | Full-automatic thrombus elastic force measuring device | |
| US20110256629A1 (en) | Fully automatic biochemical analyzer and analyzing method thereof | |
| CN104535782A (en) | Full-automatic immuno-fluorescence quantitative analysis device and implementation method | |
| CN104297498A (en) | Fully automatic biochemical and luminescent immunoassay system | |
| CN203053986U (en) | Cuvette loading structure and full-automatic analyzer | |
| CN105259357A (en) | Full-automatic chemiluminiscence tester | |
| CN110749742B (en) | POCT full-automatic chemiluminescence device based on active magnetic separation technology | |
| CN101806810A (en) | Multi-reagent needle biochemistry analyzer | |
| CN102033133A (en) | Automatic precision loading device | |
| CN206531862U (en) | Protein analyzer | |
| CN204269660U (en) | The sample transfer device of Automatic Blood Cell Analyzer | |
| CN104374935A (en) | Sampling device of fully automatic biochemical analyzer | |
| CN102445414B (en) | Test device used for medical examination | |
| CN106526211A (en) | Sampling device and electrolyte analyzer | |
| CN207623360U (en) | Analysis of protein equipment | |
| CN104897912A (en) | Biological sample analyzing device | |
| CN204269662U (en) | Automatic Blood Cell Analyzer | |
| CN209640207U (en) | A kind of atomic spectrograph sample introduction mechanism |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: No. 7 Road 518106 Guangdong Guangming New District of Shenzhen City High-tech Park West Germany Overseas Science Park A building 11 floor Patentee after: SHENZHEN ICUBIO BIOTECHNOLOGY CO.,LTD. Address before: No. 7 Road 518106 Guangdong Guangming New District of Shenzhen City High-tech Park West Germany Overseas Science Park A building 11 floor Patentee before: SHENZHEN ICUBIO BIOMEDICAL TECHNOLOGY CO.,LTD. |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150819 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |