CN205669006U - Homoiothermic rapid Solid-phase synthesis peptides mechanism - Google Patents
Homoiothermic rapid Solid-phase synthesis peptides mechanism Download PDFInfo
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- CN205669006U CN205669006U CN201620550884.2U CN201620550884U CN205669006U CN 205669006 U CN205669006 U CN 205669006U CN 201620550884 U CN201620550884 U CN 201620550884U CN 205669006 U CN205669006 U CN 205669006U
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- homoiothermic
- inner tube
- phase synthesis
- heat exchange
- synthesis peptides
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Abstract
The utility model discloses a kind of homoiothermic rapid Solid-phase synthesis peptides mechanism, including Solid-phase synthesis peptides pipe, nitrogen pot and water pump；Solid-phase synthesis peptides pipe includes inner and outer tubes, and inner tube connects downwards connecting portion wide at the top and narrow at the bottom, and the bottom of connecting portion is provided with sucking filtration mouth, and the sidewall of connecting portion is stated nitrogen pot by flexible pipe and is connected；Inner tube wall at the top of connecting portion connects core filter element, inner tube outer seal sleeve more than core filter element is provided with outer tube, outer wall of inner tube more than core filter element is provided with vertical heat exchange runner radially toward the direction depression of inner tube axis, and vertical heat exchange runner is uniformly provided with more than three along the circumferencial direction of inner tube；Bottom vertical heat exchange runner, the outer tube at place connects has homoiothermic pipeline, homoiothermic pipeline and a water tank to be connected；Outer tube top connects water return pipeline, and water return pipeline connects water tank.This utility model is added significantly to the heat exchange area of homoiothermic water and reactant liquor, can adjust reaction temperature rapidly, in time, improves reaction efficiency and reaction effect.
Description
Technical field
This utility model relates to peptide symthesis technical field, particularly relates to Solid-phase synthesis peptides technology.
Background technology
Peptide systhesis studies the brilliant course passed by more than 100 year.The 1950's, organic chemists close
Having become substantial amounts of biologically active polypeptide, including oxytocin, insulin etc., simultaneously at polypeptide synthesis method and amino acid protecting groups
The most also achieving many achievements, this is that the appearance of solid phase synthesis process later provides experiment and theoretical basis.
1963, Merrifield proposed solid-phase peptide synthesis (SPPS) first, once occurring owing to it synthesizes
Conveniently, become the prefered method of Peptide systhesis rapidly, bring the revolution on polypeptide organic synthesis, and become one
Independent subject Solid-phase organic synthesis (SPOS), the most therefore Merrifield has won the Nobel chemistry Prize of 1984.
Solid-phase synthesis peptides is to be connected on solid resin by protected amino acid, sloughs blocking group, then by the next one
Protected amino acid reacts with first amino acid whose residue and forms peptide bond, then sloughs second amino acid whose blocking group,
Reconnecting next aminoacid, so circulation goes down to make peptide chain to increase.In Solid-phase synthesis peptides, solid resin is to have to use for
Reaction substrate.
In Solid-phase synthesis peptides, it is frequently used Solid-phase synthesis peptides pipe, Solid-phase synthesis peptides pipe adds resin with anti-
The liquid solvent of activator or deprotection agent (reactant liquor i.e. dissolve in) is answered to carry out synthetic reaction.
When carrying out synthetic reaction, need reaction temperature to be controlled the most interval to improve reaction efficiency.Temperature is suitable
Increase the carrying out that can add fast response, but temperature also can accelerate the decomposition of peptide after increasing.Existing Solid-phase Polypeptide Fully automated synthesis
Instrument is expensive, and temperature regulation has certain hysteresis quality, it is difficult to adjusts reaction temperature in time, reduces reaction effect.
Utility model content
The purpose of this utility model is that providing a kind of can adjust rapidly reaction temperature and have nitrogen gas stirring function
Solid-phase synthesis peptides mechanism.
For achieving the above object, homoiothermic of the present utility model rapid Solid-phase synthesis peptides mechanism includes Solid-phase synthesis peptides
Pipe, nitrogen pot and water pump；
Solid-phase synthesis peptides pipe includes inner and outer tubes, inner tube open top and its bottom connect downwards have wide at the top and narrow at the bottom
Connecting portion, the bottom of connecting portion is provided with the sucking filtration mouth for connecting Suction filtration device, and the sidewall of connecting portion is provided with nitrogen interface, nitrogen
Interface is connected by the gas outlet of flexible pipe with described nitrogen pot；Inner tube wall at the top of connecting portion connects to be had for supporting
The core filter element of reactant, the diameter of core filter element is identical with inner tube diameter；It is provided with air outlet valve at the gas outlet of nitrogen pot；
Inner tube outer seal sleeve more than core filter element is provided with described outer tube, outer wall of inner tube more than core filter element radially towards
To the direction of inner tube axis, depression is provided with vertical heat exchange runner, and vertical heat exchange runner is uniformly provided with three along the circumferencial direction of inner tube
Above；
Bottom vertical heat exchange runner, the outer tube at place connects has homoiothermic pipeline, the bottom of homoiothermic pipeline and a water tank to be connected,
Being provided with electric heating tube in water tank, homoiothermic pipeline is provided with water pump and flow control valve；
Described outer tube top has return pipe higher than the liquid level of reactant liquor, outer tube top in the opposite side connection of homoiothermic pipeline
Road, water return pipeline connects the top of described water tank.
Described homoiothermic pipeline is provided with the first temperature sensor, is provided with the second temperature sensing in described vertical heat exchange runner
Device.
Described vertical heat exchange runner is uniformly provided with 15 along the circumferencial direction of outer tube.
Described first temperature sensor, the second temperature sensor are all connected with an electric control gear with described water pump, described
Electric control gear connects display screen.
This utility model is integrated with the spies such as nitrogen gas stirring, electrical heating, vertical heat exchange runner heat exchange, homoiothermic water recycling
Point, vertical heat exchange runner is uniformly provided with multiple along the circumferencial direction of inner tube, considerably increases the heat exchange of homoiothermic water and reactant liquor
Area, it is possible to adjust reaction temperature rapidly, in time, is very beneficial for carrying out Solid-phase synthesis peptides reaction, it is possible to increase reaction
Efficiency and reaction effect.In reaction, nitrogen is ceaselessly passed through in solvent, persistently produces the nitrogen bubble of rising, thus rise in making solvent
To sufficient stirring action, accelerate synthetic reaction.
Homoiothermic use water recycles, and can reuse heat energy contained from the water that vertical heat exchange runner is flowed out,
Compared to heating normal-temperature water, it is possible to reduce energy required during the water used in electric heater for heating water tank.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the A-A sectional drawing of Fig. 1；
Fig. 3 is electric control schematic diagram of the present utility model.
Detailed description of the invention
As shown in Figure 1, Figure 2 and Figure 3, homoiothermic of the present utility model rapid Solid-phase synthesis peptides mechanism includes Solid-phase Polypeptide
Composite tube, nitrogen pot 1 and water pump 2；
Solid-phase synthesis peptides pipe includes inner tube 3 and outer tube 4, inner tube 3 open top and its bottom connect downwards and have under upper width
Narrow connecting portion 5, the bottom of connecting portion 5 is provided with the sucking filtration mouth 6 for connecting Suction filtration device, and the sidewall of connecting portion 5 is provided with nitrogen
Interface 7, nitrogen interface 7 is connected with the gas outlet 9 of described nitrogen pot 1 by flexible pipe 8；In inner tube 3 at the top of connecting portion 5
Wall connects the core filter element 10 for supporting reactant, and the diameter of core filter element 10 is identical with inner tube 3 internal diameter；Nitrogen pot 1
Air outlet valve 11 it is provided with at gas outlet 9；Connecting portion 5 is preferably threaded in bottom inner tube 3.
Inner tube 3 outer seal sleeve of core filter element more than 10 is provided with described outer tube 4, inner tube 3 outer wall of core filter element more than 10
Direction depression radially toward inner tube 3 axis is provided with vertical heat exchange runner 12.Vertical heat exchange runner 12 is along the circumference side of inner tube 3
To being uniformly provided with more than three, it is preferably provided with 15.
Bottom vertical heat exchange runner 12, the outer tube 4 at place connects homoiothermic pipeline 13, homoiothermic pipeline 13 and the end of a water tank 14
Portion is connected, and is provided with electric heating tube 15 in water tank 14, and homoiothermic pipeline 13 is provided with water pump 2 and flow control valve 16；
Described outer tube 4 top has backwater higher than the liquid level of reactant liquor, outer tube 4 top in the opposite side connection of homoiothermic pipeline 13
Pipeline 17, water return pipeline 17 connects the top of described water tank 14.
Described homoiothermic pipeline 13 is provided with the first temperature sensor 18, is provided with the second temperature in described vertical heat exchange runner 12
Sensor 19.
Described first temperature sensor the 18, second temperature sensor 19 is all connected with an electric control gear with described water pump 2,
Described electric control gear connects display screen.Electric control gear uses PLC or integrated circuit or single-chip microcomputer.
Owing to connecting portion 5 is wide at the top and narrow at the bottom, and core filter element 10 with diameter identical with the internal diameter of inner tube 3, therefore connecting portion 5
Top also be able to play support fixation to core filter element 10, make core filter element 10 more stable inside inner tube 3.
By display screen, operator can monitor reaction temperature intuitively, in real time.According to the first temperature sensor 18
Temperature information controls the switch of electric heating tube 15, can control the leaving water temperature of water tank 14；According to the second temperature sensor 19
The open degree of temperature information regulation flow control valve 16, can control the temperature in vertical heat exchange runner 12 and inner tube 3 needing
Within the scope of Yaoing.
Each parts that this utility model relates to, such as water pump, temperature sensor, display screen etc., are this area routine techniques,
Each parts concrete structure no longer describes in detail.
During use, open electric heating tube 15, observed the temperature signal of the first temperature sensor 18 by display screen, by water tank
Lower water temperature in 14, to the temperature needed, then starts water pump 2, makes homoiothermic water be entered by homoiothermic pipeline 13 and outer tube 4
In vertical heat exchange runner 12.Homoiothermic water time vertically heat exchange runner 12 flows up, transfers heat to the reaction in inner tube 3
Liquid, and the temperature of reactant liquor is maintained preset range.Homoiothermic water is by after vertical heat exchange runner 12, by water return pipeline 17 times
It flow in water tank 14.Observe the temperature value of the second temperature sensor 19 display, when this temperature value is less than predetermined value scope, protect
Hold electric heating tube 15 in running order, and the open degree of flow control valve 16 is heightened；When this temperature value is in predetermined value
In the range of time, keep flow control valve 16 open degree；When this temperature value is higher than predetermined value scope, by flow control valve 16
Open degree turn down and be even switched off flow control valve 16.So reactant liquor can be controlled all the time in the temperature needed, for synthesis
Reaction provides good temperature conditions.
When carrying out synthetic reaction, sucking filtration mouth 6 is made to connect Suction filtration device (depression generator), by resin (solid-state) by inner tube
Inner tube 3 is put in the open top of 3, and is supported resin by core filter element 10.The solvent of activator or deprotection agent will be dissolved in again
Pour inner tube 3 into, thus carry out synthetic reaction.Open the air outlet valve 11 at nitrogen pot 1 gas outlet 9, make nitrogen be entered by inner tube 3 bottom
Enter in pipe, be then upwardly in solvent by core filter element 10.Nitrogen is ceaselessly passed through in solvent, persistently produces in making solvent
The nitrogen bubble risen, thus play sufficient stirring action, accelerate synthetic reaction.Control the open degree of air outlet valve 11, can control
The intensity of nitrogen gas stirring processed.
The air outlet valve 11 of nitrogen pot 1 is closed after reaction, under the effect of the negative pressure of Suction filtration device generation, reacted solvent
Waste liquid enters Suction filtration device by core filter element 10 and sucking filtration mouth 6.
In Solid-phase synthesis peptides reaction, need that activator and deprotection agent are used alternatingly and participate in reaction as reactant liquor.?
When changing reactant liquor, first close the air outlet valve 11 of nitrogen pot 1, then make Suction filtration device be extracted out by the waste liquid in inner tube 3, then will
Cleaning mixture adds inner tube 3 and is carried out, and the cleaning mixture after cleaning is sucked out equally under the negative pressure of Suction filtration device.After cleaning,
Inner tube 3 adds reactant liquor, starts the reaction of next stage.Alternately change activator and deprotection agent reactant liquor react,
After, waste liquid complete until reaction is drawn out of, can will take out from inner tube 31 with the resin of product (polypeptide), and complete to close
Become reaction.
Claims (4)
1. homoiothermic rapid Solid-phase synthesis peptides mechanism, it is characterised in that: include Solid-phase synthesis peptides pipe, nitrogen pot and water pump；
Solid-phase synthesis peptides pipe includes inner and outer tubes, inner tube open top and connect downwards bottom it and have connection wide at the top and narrow at the bottom
Portion, the bottom of connecting portion is provided with the sucking filtration mouth for connecting Suction filtration device, and the sidewall of connecting portion is provided with nitrogen interface, nitrogen interface
It is connected by the gas outlet of flexible pipe with described nitrogen pot；Inner tube wall at the top of connecting portion connects to be had for supporting reaction
The core filter element of thing, the diameter of core filter element is identical with inner tube diameter；It is provided with air outlet valve at the gas outlet of nitrogen pot；
Inner tube outer seal sleeve more than core filter element is provided with described outer tube, and outer wall of inner tube more than core filter element is radially towards inwardly
The direction depression of pipe axis is provided with vertical heat exchange runner, vertical heat exchange runner along the circumferencial direction of inner tube be uniformly provided with three with
On；
Bottom vertical heat exchange runner, the outer tube at place connects has homoiothermic pipeline, the bottom of homoiothermic pipeline and a water tank to be connected, water tank
In be provided with electric heating tube, homoiothermic pipeline is provided with water pump and flow control valve；
There is water return pipeline at described outer tube top in the opposite side connection of homoiothermic pipeline higher than the liquid level of reactant liquor, outer tube top, returns
Water lines connects the top of described water tank.
Homoiothermic the most according to claim 1 rapid Solid-phase synthesis peptides mechanism, it is characterised in that: on described homoiothermic pipeline
It is provided with the first temperature sensor, in described vertical heat exchange runner, is provided with the second temperature sensor.
Homoiothermic the most according to claim 2 rapid Solid-phase synthesis peptides mechanism, it is characterised in that: described vertical heat exchange stream
Road is uniformly provided with 15 along the circumferencial direction of outer tube.
4. according to the homoiothermic described in Claims 2 or 3 rapid Solid-phase synthesis peptides mechanism, it is characterised in that: described first temperature
Degree sensor, the second temperature sensor are all connected with an electric control gear with described water pump, and described electric control gear connects display
Screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620550884.2U CN205669006U (en) | 2016-06-08 | 2016-06-08 | Homoiothermic rapid Solid-phase synthesis peptides mechanism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620550884.2U CN205669006U (en) | 2016-06-08 | 2016-06-08 | Homoiothermic rapid Solid-phase synthesis peptides mechanism |
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| Publication Number | Publication Date |
|---|---|
| CN205669006U true CN205669006U (en) | 2016-11-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620550884.2U Expired - Fee Related CN205669006U (en) | 2016-06-08 | 2016-06-08 | Homoiothermic rapid Solid-phase synthesis peptides mechanism |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676056A (en) * | 2018-06-19 | 2018-10-19 | 南京肽业生物科技有限公司 | Polypeptides reactive synthesis unit |
| CN108912206A (en) * | 2018-06-19 | 2018-11-30 | 南京肽业生物科技有限公司 | Peptide systhesis device |
-
2016
- 2016-06-08 CN CN201620550884.2U patent/CN205669006U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676056A (en) * | 2018-06-19 | 2018-10-19 | 南京肽业生物科技有限公司 | Polypeptides reactive synthesis unit |
| CN108912206A (en) * | 2018-06-19 | 2018-11-30 | 南京肽业生物科技有限公司 | Peptide systhesis device |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161102 Termination date: 20170608 |
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| CF01 | Termination of patent right due to non-payment of annual fee |