CN205088163U - Controllable polypeptide solid -phase synthesis system of temperature - Google Patents
Controllable polypeptide solid -phase synthesis system of temperature Download PDFInfo
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- CN205088163U CN205088163U CN201520875990.3U CN201520875990U CN205088163U CN 205088163 U CN205088163 U CN 205088163U CN 201520875990 U CN201520875990 U CN 201520875990U CN 205088163 U CN205088163 U CN 205088163U
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Abstract
The utility model discloses a controllable polypeptide solid -phase synthesis system of temperature, including polypeptide solid -phase synthesis pipe, erlenmeyer flask, nitrogen gas jar, vacuum pump and two three -way valves, the bottom of polypeptide solid -phase synthesis pipe is connected with the first interface of two three -way valves, the second interface of two three -way valves passes through the gas outlet of nitrogen gas hose connection nitrogen gas jar, the third interface fixedly connected with of two three -way valves goes out liquid glass pipe, goes out liquid glass pipe the top seal downward and erlenmeyer flask and communicates, the upper portion of erlenmeyer flask is equipped with connection for vacuum pump, and connection for vacuum pump is connected with the vacuum pump through the vacuum tube way, polypeptide solid -phase synthesis outside of tubes cover is equipped with the water jacket, and one side bottom of water jacket is connected with the inlet tube and just is connected with the outlet pipe in its opposite side top, be equipped with the water pump on the inlet tube. The utility model discloses the polypeptide solid -phase synthesis reaction is very conveniently carried out to the functions such as nitrogen gas stirring, negative pressure absorption waste liquid that have integrateed, can be through the water jacket with the temperature of polypeptide solid -phase synthesis pipe control all the time at needs.
Description
Technical field
The utility model relates to peptide symthesis technical field, particularly relates to a kind of Solid-phase synthesis peptides system.
Background technology
Peptide systhesis research has been passed by the brilliant course of more than 100 year.The 1950's; organic chemists have synthesized a large amount of biologically active polypeptidess, comprise pitocin, Regular Insulin etc.; simultaneously on polypeptide synthesis method and amino acid protecting groups, also achieve many achievements, this be that the appearance of solid phase synthesis process afterwards provides and tests and theoretical basis.
1963, Merrifield proposes solid-phase peptide synthesis (SPPS) first, the prefered method of Peptide systhesis is become once occurring because its synthesis is convenient, rapidly, bring the revolution in polypeptide organic synthesis, and becoming an independently subject---Solid-phase organic synthesis (SPOS), therefore Merrifield has also won the Nobel chemistry Prize of 1984.
Solid-phase synthesis peptides is connected on solid resin by protected amino acid; slough blocking group; then next protected amino acid and first amino acid whose residue are reacted and form peptide bond; then second amino acid whose blocking group is sloughed; connect next amino acid again, circulation goes down to make peptide chainpropagation like this.In Solid-phase synthesis peptides, solid resin is the reaction substrate that must use.
In Solid-phase synthesis peptides, need temperature to control in suitable interval.Temperature suitably increases the carrying out that can add fast response, but also can accelerate the decomposition of peptide after increase in temperature.Market there is no the Solid-phase synthesis peptides device that can control temperature of reaction in reaction process flexibly.
Existing Solid-phase Polypeptide Fully automated synthesis instrument is expensive, market needs a kind of easy to use, structure simple, Solid-phase synthesis peptides system easy to utilize.
Utility model content
The purpose of this utility model be to provide a kind of structure simple, there is nitrogen gas stirring function, and water jacket can be utilized to control the Solid-phase synthesis peptides system of temperature of reaction.
For achieving the above object, the Solid-phase synthesis peptides system of temperature-controllable of the present utility model comprises Solid-phase synthesis peptides pipe, Erlenmeyer flask, nitrogen pot, vacuum pump and makes Solid-phase synthesis peptides pipe select to be communicated with the two-position three-way valve of nitrogen pot or Erlenmeyer flask;
Solid-phase synthesis peptides pipe open top and the core filter core be provided with bottom it for supporting reactant; The bottom of Solid-phase synthesis peptides pipe is connected with the first interface of two-position three-way valve;
Second interface of two-position three-way valve passes through the air outlet of the hose connection described nitrogen pot of nitrogen;
3rd interface of two-position three-way valve is fixedly connected with fluid Glass tubing, and fluid Glass tubing is communicated with the top seal of Erlenmeyer flask downwards; The top of Erlenmeyer flask is provided with and vacuumizes interface, vacuumizes interface and is connected with described vacuum pump for vacuumizing Erlenmeyer flask by vacuum-pumping pipeline;
Be arranged with water jacket outside described Solid-phase synthesis peptides pipe, a side bottom of water jacket is connected with water inlet pipe and its opposite side top is connected with rising pipe; Water inlet pipe is provided with the water pump for being pumped from water source by water to water jacket.
The air outlet of nitrogen pot is provided with air outlet valve.
The utility model passes through two-position three-way valve, Solid-phase synthesis peptides pipe is enable to select to be communicated with nitrogen pot or Erlenmeyer flask, so only need operation two-position three-way valve can select carry out nitrogen gas stirring or carry out waste collection, need not carry out plugging and being connected, very convenient use temporarily.In the utility model, the top of Erlenmeyer flask is connected with vacuum pump, can form negative pressure easily in Erlenmeyer flask, thus makes the waste liquid in Solid-phase synthesis peptides pipe penetrate core filter core under the influence of air pressure and enter in Erlenmeyer flask, facilitates the collection of waste liquid.In a word, the utility model is integrated with the function such as nitrogen gas stirring, negative pressure absorbing waste liquid, very conveniently carries out Solid-phase synthesis peptides reaction.
The setting of air outlet valve, can control the break-make of nitrogen on the one hand, and on the other hand, can control the open degree of air outlet valve as required thus the intensity of control nitrogen gas stirring, staff can by the intensity control of nitrogen gas stirring in the degree be applicable to the most.
According to synthesis need control the temperature at water source, Solid-phase synthesis peptides pipe can be controlled all the time the temperature that needing, for building-up reactions provides good temperature condition by water jacket.Finally, the utility model structure is simple, easy to use, is convenient to manufacture and be connected use.
Accompanying drawing explanation
Fig. 1 is the structural representation under the utility model using state;
Fig. 2 is the structural representation of two-position three-way valve;
Fig. 3 is the structural representation of Erlenmeyer flask.
Embodiment
As shown in Figure 1, Figure 2 and Figure 3, the Solid-phase synthesis peptides system of temperature-controllable of the present utility model comprises Solid-phase synthesis peptides pipe 1, Erlenmeyer flask 2, nitrogen pot 3, waste liquid cylinder 4, vacuum pump 5 and makes Solid-phase synthesis peptides pipe 1 select to be communicated with the two-position three-way valve 6 of nitrogen pot 3 or Erlenmeyer flask 2.
Solid-phase synthesis peptides pipe 1 open top, bottom is provided with the core filter core 7 for supporting reactant; The bottom of the Solid-phase synthesis peptides pipe 1 below core filter core 7 is connected with the first interface 8 of two-position three-way valve 6.
Second interface 9 of two-position three-way valve 6 connects the air outlet 12 of described nitrogen pot 3 by nitrogen flexible pipe 11, the air outlet 12 of nitrogen pot 3 is provided with air outlet valve 13.
3rd interface 10 of two-position three-way valve 6 is fixedly connected with fluid Glass tubing 14, and fluid Glass tubing 14 is communicated with the top seal of Erlenmeyer flask 2 downwards; The top of Erlenmeyer flask 2 is provided with and vacuumizes interface 15, vacuumizes interface 15 and is connected with described vacuum pump 5 for vacuumizing Erlenmeyer flask 2 by vacuum-pumping pipeline 16.
Be arranged with water jacket 17 outside described Solid-phase synthesis peptides pipe 1, a side bottom of water jacket 17 is connected with water inlet pipe 18 and its opposite side top is connected with rising pipe 19; Water inlet pipe 18 is provided with the water pump 20 for being pumped from water source by water to water jacket 17;
Fluid Glass tubing 14 is communicated with by following sealing structure with the top of Erlenmeyer flask 2:
The top of described Erlenmeyer flask 2 is provided with the ground 21 for feed liquor, the taper that ground 21 is big up and small down and its internal surface is frosting; The bottom of fluid Glass tubing 14 is integrally formed with glass stopper 22, and the middle part of glass stopper 22 is provided with the centre hole 23 running through glass stopper 22 up and down, and centre hole 23 communicates with fluid Glass tubing 14; The outside surface of glass stopper 22 is frosting and in the up big and down small conical surface suitable with described ground 21, and glass stopper 22 to be plugged in ground 21 and to be sealed and matched with ground 21 internal surface.
The bottom of described Erlenmeyer flask 2 is provided with liquid outlet 24, and liquid outlet 24 is connected with fluid flexible pipe 25, and the free end of fluid flexible pipe 25 passes into waste liquid cylinder 4; Fluid flexible pipe 25 is provided with clip or liquid-outlet valve 26; Waste liquid cylinder 4 top is lower than the bottom of Erlenmeyer flask 2, so that the waste liquid in Erlenmeyer flask 2 flows out smoothly.
Wherein,
solid-phase synthesis peptides pipe 1, two-position three-way valve 6, nitrogen pot 3, vacuum pump 5, water pump 20 and core filter core 7 etc. are existing conventional components, and its concrete structure no longer describes in detail.
Erlenmeyer flask 2 top is provided with ground 21,3rd interface of two-position three-way valve 6 is connected Erlenmeyer flask 2 by fluid Glass tubing with glass stopper 22, the outside surface of glass stopper 22 and the internal surface of Erlenmeyer flask 2 are mutually adaptive taper frosting, thus very socket connection is convenient to, after linking together, that can pass through frosting between ground with glass stopper coordinates self-assembling formation sealed state.This set is both convenient to be connected, and can avoid again in the past when Glass tubing being inserted cork stopper or rubber plug, Glass tubing occurs and to fracture the phenomenon damaged.
The liquid outlet 24 of Erlenmeyer flask 2 is connected with fluid flexible pipe 25, and the free end of fluid flexible pipe passes into waste liquid cylinder 4.Adopt this set, in Erlenmeyer flask, have accumulated more waste liquid, when needing cleaning, just need not again Erlenmeyer flask be separated with miscellaneous equipment, but only need to take off clip or open liquid-outlet valve and can make waste liquid inflow waste liquid cylinder, further facilitate use.
During use, by the lower water temperature at water source to the temperature needed, water inlet pipe 18 is connected greatly with the water outlet at water source, rising pipe 19 is connected with the water-in at water source, thus form circulator bath pipeline.Certainly, the water in rising pipe 19 also can flow into water drain as waste water.Open water pump 20, the water in water source is pumped to water jacket 17 1 side bottom, by being flowed out by the top of water jacket 17 opposite side after water jacket 17.According to the temperature needing control water source of synthesis, Solid-phase synthesis peptides pipe 1 can be controlled all the time the temperature needed, for building-up reactions provides good temperature condition.
Resin (solid-state) is put into pipe by the open top of Solid-phase synthesis peptides pipe 1, and supports resin by core filter core 7.Pour the solvent dissolving in activator or deprotection agent into Solid-phase synthesis peptides pipe 1 again, thus carry out building-up reactions.Control the working order of two-position three-way valve 6, its first interface 8 is connected with the second interface 9, and then makes Solid-phase synthesis peptides pipe 1 be communicated with nitrogen pot 3.Open the air outlet valve 13 at nitrogen pot 3 air outlet 12 place, make nitrogen enter in pipe by Solid-phase synthesis peptides pipe 1 bottom, then upwards entered in solvent by core filter core 7.Nitrogen ceaselessly passes in solvent, makes to continue in solvent to produce the nitrogen bubble risen, thus plays sufficient stirring action.Control the open degree of air outlet valve 13, the intensity of nitrogen gas stirring can be controlled.
Close the air outlet valve 13 of nitrogen pot 3 after reaction, regulate the working order of two-position three-way valve 6, its first interface 8 is connected with the 3rd interface 10, and then makes Solid-phase synthesis peptides pipe 1 be communicated with Erlenmeyer flask 2.Open vacuum pump 5 pairs of Erlenmeyer flasks 2 to vacuumize; Under the effect of negative pressure, reacted solvent waste liquid enters Erlenmeyer flask 2 by core filter core 7, two-position three-way valve 6 and ground 21, accumulates in the bottom of Erlenmeyer flask 2.Waste liquid accumulate in Erlenmeyer flask 2 more after, Erlenmeyer flask 2 can be taken off from the utility model that (extracting glass stopper 22) is manual afterwards pours waste liquid into waste liquid cylinder 4.When being provided with fluid flexible pipe 25, only needing to take off clip from fluid flexible pipe 25 or open liquid-outlet valve 26, waste liquid can be made naturally to flow into waste liquid cylinder 4 under gravity, use very convenient.Waste liquid flow out after, then by clip on fluid flexible pipe 25 or close liquid-outlet valve 26.
In Solid-phase synthesis peptides reaction, need to be used alternatingly activator and deprotection agent participates in reaction as reaction solution.When changing reaction solution, first closing the air outlet valve 13 of nitrogen pot 3, regulating the working order of two-position three-way valve 6, its first interface 8 being connected with the 3rd interface 10, and then making Solid-phase synthesis peptides pipe 1 be communicated with Erlenmeyer flask 2.Then start vacuum pump 5, by the waste liquid suction Erlenmeyer flask 2 in Solid-phase synthesis peptides pipe 1, then washings is added Solid-phase synthesis peptides pipe 1 and clean, the washings after cleaning is inhaled into Erlenmeyer flask 2 equally under the negative pressure of vacuum pump 5 generation.After cleaning, close vacuum pump 5, again regulate two-position three-way valve 6, Solid-phase synthesis peptides pipe 1 is connected with nitrogen pot 3; Add reaction solution at Solid-phase synthesis peptides pipe 1, start the reaction of next stage.Alternately replacing activator and deprotection agent reaction solution react; until react complete, after waste liquid is drawn out of Solid-phase synthesis peptides pipe 1; can take out in Solid-phase synthesis peptides pipe 1 with the resin of reaction product (polypeptide), complete repertoire of the present utility model.
Above embodiment is the unrestricted the technical solution of the utility model in order to explanation only, although be described in detail the utility model with reference to above-described embodiment, those of ordinary skill in the art is to be understood that: still can modify to the utility model or equivalent replacement, and not departing from any modification or partial replacement of spirit and scope of the present utility model, it all should be encompassed in the middle of right of the present utility model.
Claims (2)
1. the Solid-phase synthesis peptides system of temperature-controllable, is characterized in that: comprise Solid-phase synthesis peptides pipe, Erlenmeyer flask, nitrogen pot, vacuum pump and make Solid-phase synthesis peptides pipe select to be communicated with the two-position three-way valve of nitrogen pot or Erlenmeyer flask;
Solid-phase synthesis peptides pipe open top and the core filter core be provided with bottom it for supporting reactant; The bottom of Solid-phase synthesis peptides pipe is connected with the first interface of two-position three-way valve;
Second interface of two-position three-way valve passes through the air outlet of the hose connection described nitrogen pot of nitrogen;
3rd interface of two-position three-way valve is fixedly connected with fluid Glass tubing, and fluid Glass tubing is communicated with the top seal of Erlenmeyer flask downwards; The top of Erlenmeyer flask is provided with and vacuumizes interface, vacuumizes interface and is connected with described vacuum pump for vacuumizing Erlenmeyer flask by vacuum-pumping pipeline;
Be arranged with water jacket outside described Solid-phase synthesis peptides pipe, a side bottom of water jacket is connected with water inlet pipe and its opposite side top is connected with rising pipe; Water inlet pipe is provided with the water pump for being pumped from water source by water to water jacket.
2. the Solid-phase synthesis peptides system of temperature-controllable according to claim 1, is characterized in that: the air outlet of nitrogen pot is provided with air outlet valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201520875990.3U CN205088163U (en) | 2015-11-06 | 2015-11-06 | Controllable polypeptide solid -phase synthesis system of temperature |
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| CN201520875990.3U CN205088163U (en) | 2015-11-06 | 2015-11-06 | Controllable polypeptide solid -phase synthesis system of temperature |
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| CN205088163U true CN205088163U (en) | 2016-03-16 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106732229A (en) * | 2016-12-03 | 2017-05-31 | 河南工程学院 | Spherical Solid-phase Polypeptide reaction bulb |
| CN106986916A (en) * | 2017-03-20 | 2017-07-28 | 珠海百世瑞生命科技有限公司 | Full automatic high efficiency solid phase peptide synthesizer and application method |
| CN107722104A (en) * | 2016-08-23 | 2018-02-23 | 安徽天达谱申生物科技有限公司 | A kind of microwave Peptide synthesizer |
| CN108623651A (en) * | 2018-06-19 | 2018-10-09 | 南京肽业生物科技有限公司 | Efficient Peptide systhesis device |
| CN108676057A (en) * | 2018-06-19 | 2018-10-19 | 南京肽业生物科技有限公司 | Solid phase peptide synthssis device |
| CN108676056A (en) * | 2018-06-19 | 2018-10-19 | 南京肽业生物科技有限公司 | Polypeptides reactive synthesis unit |
| CN108912208A (en) * | 2018-06-20 | 2018-11-30 | 南京肽业生物科技有限公司 | A kind of adjustable Solid-phase synthesis peptides device of temperature |
| CN108912206A (en) * | 2018-06-19 | 2018-11-30 | 南京肽业生物科技有限公司 | Peptide systhesis device |
| CN108912207A (en) * | 2018-06-19 | 2018-11-30 | 南京肽业生物科技有限公司 | High-throughput Solid phase peptide synthssis device |
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2015
- 2015-11-06 CN CN201520875990.3U patent/CN205088163U/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107722104A (en) * | 2016-08-23 | 2018-02-23 | 安徽天达谱申生物科技有限公司 | A kind of microwave Peptide synthesizer |
| CN107722104B (en) * | 2016-08-23 | 2023-11-17 | 南京金斯瑞生物科技有限公司 | Microwave polypeptide synthesizer |
| CN106732229A (en) * | 2016-12-03 | 2017-05-31 | 河南工程学院 | Spherical Solid-phase Polypeptide reaction bulb |
| CN106732229B (en) * | 2016-12-03 | 2019-03-29 | 河南工程学院 | Spherical Solid-phase Polypeptide reaction flask |
| CN106986916A (en) * | 2017-03-20 | 2017-07-28 | 珠海百世瑞生命科技有限公司 | Full automatic high efficiency solid phase peptide synthesizer and application method |
| CN106986916B (en) * | 2017-03-20 | 2023-10-13 | 珠海百世瑞生命科技有限公司 | Full-automatic high-efficiency solid-phase polypeptide synthesizer and use method thereof |
| CN108623651A (en) * | 2018-06-19 | 2018-10-09 | 南京肽业生物科技有限公司 | Efficient Peptide systhesis device |
| CN108676057A (en) * | 2018-06-19 | 2018-10-19 | 南京肽业生物科技有限公司 | Solid phase peptide synthssis device |
| CN108676056A (en) * | 2018-06-19 | 2018-10-19 | 南京肽业生物科技有限公司 | Polypeptides reactive synthesis unit |
| CN108912206A (en) * | 2018-06-19 | 2018-11-30 | 南京肽业生物科技有限公司 | Peptide systhesis device |
| CN108912207A (en) * | 2018-06-19 | 2018-11-30 | 南京肽业生物科技有限公司 | High-throughput Solid phase peptide synthssis device |
| CN108912208A (en) * | 2018-06-20 | 2018-11-30 | 南京肽业生物科技有限公司 | A kind of adjustable Solid-phase synthesis peptides device of temperature |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160316 Termination date: 20171106 |
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| CF01 | Termination of patent right due to non-payment of annual fee |