CN101403758A - Fast detection reagent kit for melamine - Google Patents
Fast detection reagent kit for melamine Download PDFInfo
- Publication number
- CN101403758A CN101403758A CNA2008100463084A CN200810046308A CN101403758A CN 101403758 A CN101403758 A CN 101403758A CN A2008100463084 A CNA2008100463084 A CN A2008100463084A CN 200810046308 A CN200810046308 A CN 200810046308A CN 101403758 A CN101403758 A CN 101403758A
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- Prior art keywords
- melamine
- strip
- detection
- sample
- kit
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- 229920000877 Melamine resin Polymers 0.000 title claims abstract description 91
- 238000001514 detection method Methods 0.000 title claims abstract description 82
- JDSHMPZPIAZGSV-UHFFFAOYSA-N melamine Chemical compound NC1=NC(N)=NC(N)=N1 JDSHMPZPIAZGSV-UHFFFAOYSA-N 0.000 title claims abstract description 73
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 13
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 43
- 238000012360 testing method Methods 0.000 claims abstract description 38
- 238000003908 quality control method Methods 0.000 claims abstract description 36
- 239000004033 plastic Substances 0.000 claims abstract description 34
- 239000010931 gold Substances 0.000 claims abstract description 27
- 229910052737 gold Inorganic materials 0.000 claims abstract description 27
- 239000000084 colloidal system Substances 0.000 claims abstract description 24
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 21
- 238000005070 sampling Methods 0.000 claims abstract description 20
- 239000000427 antigen Substances 0.000 claims abstract description 19
- 102000036639 antigens Human genes 0.000 claims abstract description 19
- 108091007433 antigens Proteins 0.000 claims abstract description 19
- 238000009434 installation Methods 0.000 claims abstract description 7
- 241000283707 Capra Species 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000000020 Nitrocellulose Substances 0.000 claims description 20
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 claims description 20
- 239000003365 glass fiber Substances 0.000 claims description 20
- 239000012528 membrane Substances 0.000 claims description 20
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 19
- 238000005516 engineering process Methods 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 12
- 230000000274 adsorptive effect Effects 0.000 claims description 7
- 238000001291 vacuum drying Methods 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- ALSTYHKOOCGGFT-KTKRTIGZSA-N (9Z)-octadecen-1-ol Chemical compound CCCCCCCC\C=C/CCCCCCCCO ALSTYHKOOCGGFT-KTKRTIGZSA-N 0.000 claims description 3
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 3
- 239000000853 adhesive Substances 0.000 claims description 3
- 230000001070 adhesive effect Effects 0.000 claims description 3
- 239000011888 foil Substances 0.000 claims description 3
- 229940055577 oleyl alcohol Drugs 0.000 claims description 3
- XMLQWXUVTXCDDL-UHFFFAOYSA-N oleyl alcohol Natural products CCCCCCC=CCCCCCCCCCCO XMLQWXUVTXCDDL-UHFFFAOYSA-N 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- JAJWGJBVLPIOOH-IZYKLYLVSA-M sodium taurocholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 JAJWGJBVLPIOOH-IZYKLYLVSA-M 0.000 claims description 3
- 239000012064 sodium phosphate buffer Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 6
- 239000011152 fibreglass Substances 0.000 abstract 2
- 239000000523 sample Substances 0.000 description 61
- 235000013305 food Nutrition 0.000 description 9
- 239000007788 liquid Substances 0.000 description 5
- 239000012472 biological sample Substances 0.000 description 4
- 235000013365 dairy product Nutrition 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000013098 chemical test method Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- XZMCDFZZKTWFGF-UHFFFAOYSA-N Cyanamide Chemical compound NC#N XZMCDFZZKTWFGF-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 239000003317 industrial substance Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention provides a melamine detection kit, comprising a plastic shell, a melamine detection test strip and a sampling sucker which is attached to the kit; and the plastic shell is divided into a front part and a rear part. The upper surface of the front part sinks to form a groove used for receiving the sampling sucker; the notch is attached with plastic wrappage so as to prevent the sampling sucker from dropping off during the transportation process of the kit; the rear part comprises an upper cover and a lower cover; and the upper cover is provided with a sample-adding hole, a detection display window and an installation marking hole. The lower cover of the rear part is internally provided with a test strip groove, and the test strip is installed in the groove. The test strip is sequentially provided with a sample pad, a fiberglass film combination pad, a nitrocellulose film with detection belts and quality control belts, a strong water absorbing pad and an installation marking paper label. The fiberglass film combination pad of the test strip is provided with an anti-melamine monoclonal antibody and an anti-rabbit antibody marked by colloid gold; the quality control belt is provided with a goat anti-rabbit polyclonal antibody; and the detection belt is provided with melamine antigen. The kit has high exactness and reliability for detecting the melamine in the sample, and has simple and quick detection process and convenient carrying.
Description
Technical field
The present invention relates to a kind of melamine detection kit and preparation technology thereof, specifically, is that the specific antibody of anti-melamine is produced in the detection kit, is used for whether containing tripolycyanamide in the detection of biological sample.
Background technology
Melamine is a kind of nitrogenous industrial chemicals, adds melamine in the food and can improve the Protein Detection value but contaminated food products, can cause urinary system to produce calculus after the human body edible tri poly cyanamid contaminated food products.Current, some lawless persons add melamine to improve the dairy protein detected value for seeking unlawful interests in dairy products, brought serious harm for people's life health, have caused baneful influence to society.Therefore, especially carrying out the tripolycyanamide fast detecting in the food in biomedical sample has very important significance.
At present, in the biomedical sample especially in the food sanitation safe melamine detect and still do not have fast method.Colloidal gold immunochromatographimethod technology (Gold-Immunochromatography Assay, GICA) be an immunolabelling technique that on the basis of ELISA adsorption analysis method (ELISA), grows up, it is carrier with the miillpore filter, utilize the capillarity of miillpore filter, dropping is slowly oozed to the miillpore filter other end at the fluid sample of film bar one side to move, if specific antigen or antibody are arranged in the liquid, they can with the immunity gold in conjunction with after again be fixed on miillpore filter on corresponding antibodies or antigen combine and show immune golden color.This technology simply, fast, does not need any instrument and equipment, and a few minutes result that just can detect by an unaided eye is called " concentrated ELISA " by praise.Detect and still do not have this problem of fast method at present for solving in the biomedical sample especially in the food melamine, the invention provides a kind of fast detection reagent kit for melamine and kit preparation technology thereof based on the colloidal gold immunochromatographimethod technology.
Summary of the invention
The technical problem to be solved in the present invention
In view of in the present biomedical sample especially in the food sanitation safe melamine still do not have method for quick, for this reason, the present invention:
1, provide a kind of melamine quick on-the-spot detection kit.
2, the preparation technology of the quick on-the-spot detection kit of a kind of melamine is provided.
Technical scheme of the present invention is:
Described melamine detection kit comprises plastic casing 1, melamine detection test strip 13 and a stopple coupon 5 that is attached on the kit box body:
Described plastic casing 1 footpath upwards is divided into housing previous section 2 and housing aft section 3; Described housing 1 previous section 2 its upper surfaces sink to form a groove 4, and the sampling pipette of using for sample detection 5 is joined and is put in the groove, notch with plastic wrappage 6 sampling pipette 5 is encapsulated in the sampling pipette groove 4 in case kit in transit sampling pipette 5 drop;
Described housing 1 aft section 3 comprises dismountable loam cake 7 and lower cover 8, radially has well 9, testing result display window 10 on the loam cake 7 successively and sign hole 11 is installed; Lower cover 8 inner radial are provided with strip groove 12; Described strip 13 is radially chimeric to be installed in this strip groove 12;
Described strip 13 is provided with sample pad 14, glass fibre membrane pad 15 that mutual overlap joint pastes in turn, have detect with 16 and the cellulose nitrate analyzing film 18 of quality control band 17, strong adsorptive pads 19 and strip sign paper be installed signed 20;
The glass fibre membrane pad 15 of described strip is provided with the monoclonal antibody of anti-melamine of colloid gold label and the rabbit antibody of colloid gold label; Have detect with 16 and the cellulose nitrate analyzing film 18 of quality control band 17 on detection be with 16 to be provided with melamine antigen, quality control band 17 is provided with the goat-anti rabbit polyclonal antibody;
Be installed in the melamine detection test strip 13 in the lower cover internal recess 12 of plastic casing 1 aft section 3, sample pad 14 on it, have detect with 16 and the cellulose nitrate analyzing film 18 of quality control band 17 and strip sign paper be installed signed 20 respectively with well 9, the testing result display window 10 of plastic casing loam cake 7 with sign hole 11 corresponding placements are installed, can see strip installation sign paper label 20 by the installation sign hole 11 of plastic casing loam cake 7, whether correctly install to determine strip 13.
During sample detection, tear the plastic wrappage 6 that covers on the sampling pipette groove 4, take out sampling pipette 5, draw sample liquid to be checked with this sampling pipette 5, dropping 3-4 drips and treats corpse or other object for laboratory examination and chemical testing sample liquid in the sample pipetting volume hole 9 of described kit, the 3-5 minute testing result that can detect by an unaided eye.
Melamine detection kit test sample tripolycyanamide provided by the invention adopts competition law.
If do not contain tripolycyanamide in the sample, the anti-melamine monoclonal antibody gold mark thing that is provided with on the strip glass fibre membrane pad 15 oozes the detection that moves on to strip cellulose nitrate analyzing film when being with 16 positions with sample, anti-melamine monoclonal antibody on the colloidal gold particle will be arranged on the melamine antigen that strip cellulose nitrate analyzing film detects with 16 positions and combine, and assemble red zone of formation, this shows that testing result is negative.
If contain tripolycyanamide in the sample, the limited anti-melamine monoclonal antibody that they will compete colloid gold label on the strip with the set melamine antigen in 16 positions with the detection of strip cellulose nitrate analyzing film, and combine with it.When the concentration of the tripolycyanamide in the sample to be checked meets or exceeds when a certain amount of, tripolycyanamide in the sample will combine with the limited anti-melamine monoclonal antibody of strip colloid gold label, thereby make and be arranged on strip and detect with the melamine antigen on 16 and can't combine with the anti-melamine monoclonal antibody of colloid gold label, being provided with the detection of melamine antigen this moment is with 16 red zone can not occur, this shows in this sample and contains tripolycyanamide that testing result is positive.
The glass fibre membrane pad 15 of the strip of melamine detection kit provided by the invention is provided with the colloid gold label thing of rabbit antibody.This label together oozes when moving to quality control band 17 with sample, can react with the goat-anti rabbit polyclonal antibody that quality control band 17 is provided with (the colloid gold label thing of this rabbit antibody can not react with 16 melamine antigen that are provided with detection), quality control band 17 position display red zones also can be identified by naked eyes.When in the sample detection because certain constituent mass problem can't detect when test, strip is provided with quality control band 17 positions of goat-anti rabbit polyclonal antibody and then can develop the color, so, can be to sample melamine detection carrying out quality control by the Quality Control thing goat-anti rabbit polyclonal antibody that strip quality control band 17 is provided with.Quality control band 17 redfree bands show that testing result is invalid.
The present invention also provides a kind of preparation technology of melamine detection kit, and it may further comprise the steps:
A, handle sample pad 14 with closed reagent.The prescription of described closed reagent is: sodium taurocholate 0.1-0.7%, Tween-20 0.05-1%, polyethoxy oleyl alcohol 0.5-2%, sodium chloride 0.5-1.2%, pure water 95-99%, sodium phosphate (PB) damping fluid 0.01-0.03mmol/L;
B, configuration colloidal gold solution:
Regulate the pH of colloidal gold solution to selected standard with the sal tartari of 0.2M or the hydrochloric acid of 0.1M; The monoclonal antibody of volume is an amount of, that concentration is suitable anti-melamine becomes colloidal gold solution with rabbit antibody rapid mixing; The colloidal gold solution point that is disposed is added on the glass fibre membrane, forms the glass fibre membrane pad 15 of colloid gold label;
C, configuration melamine antigen solution and configuration goat anti-rabbit antibody solution form detection with some film instrument to the cellulose nitrate analyzing film 18 with their speckings respectively separated by a distance then and are with 16 and quality control band 17;
D, respectively sample pad 14, point are added with the colloid gold label thing glass fibre membrane pad 15, have detect with 16 and the cellulose nitrate analyzing film 18 of quality control band 17, strong adsorptive pads 19 and strip install sign paper sign 20 successively mutually overlap joint paste on the offset plate of an adhesive sticker, cut into the strip of certain-length and width afterwards, and strip is installed in the groove 12 in the plastic casing aft section lower cover 8;
E, vacuum drying: the temperature in the vacuum pump is controlled at 15-25 ℃; The vacuum drying time was controlled at 2-5 hour; Atmospheric pressure in the vacuum pump is controlled at below 0.1 millibar (mba).
F, foil sealing packing are promptly finished a melamine detection kit and are made.
Beneficial effect of the present invention
Melamine detection kit provided by the invention and preparation technology thereof have the following advantages:
1, described kit is simple in structure, easily makes, and is with low cost, is particularly suitable for suitability for industrialized production.
2, described kit is used for the test sample tripolycyanamide fast, accurately, and is easy and simple to handle, easy to carry.Detection suitablely all can be used without any need for instrument and equipment and reagent in good time.Operation does not need special study and training, and professional and technical personnel and ordinary person all are suitable for.During sample detection, operating personnel only need tear the plastic wrappage 6 of kit surface sample suction pipe 5 off, take out sample suction pipe 5, suction pipe 5 is directly used in draws sample to be checked and drip after sample to be checked goes into the well 9 of kit, just can obtain testing result in 3-5 minute.
3, reagent and the material in the described kit do not relate to active inactivation, can be in the room temperature long preservation.
4, described kit has good practicality and market popularization value, can be used for biological sample, especially the tripolycyanamide field quick detection and the extensive examination of dairy products in the food sanitation safe etc.
Description of drawings
Fig. 1 does not dismantle the structural representation of the loam cake of plastic casing aft section for melamine detection kit of the present invention.
Fig. 2 is the structural representation behind the loam cake of melamine detection kit of the present invention dismounting plastic casing aft section.
Fig. 3 is the side-looking structural drawing of the strip of melamine detection kit of the present invention.
Fig. 4 is the plan structure figure of the strip of melamine detection kit of the present invention.
Fig. 5 is used for detection of biological test result of samples synoptic diagram for melamine detection kit of the present invention.Fig. 5 represents that sample does not contain melamine.
Fig. 6 is used for detection of biological test result of samples synoptic diagram for melamine detection kit of the present invention.Fig. 6 represents that sample contains melamine.
Sequence number is expressed as follows: 1, plastic casing 2, plastic casing previous section 3, plastic casing aft section 4, plastic casing previous section upper surface sink to form is used to join the groove 5 that takes the sample suction pipe, sampling pipette 6, plastic wrappage 7, the loam cake 8 of plastic casing aft section, the lower cover 9 of plastic casing aft section, well 10, testing result display window 11, sign hole 12 is installed, strip groove 13, melamine detection test strip 14, sample pad 15, glass fibre membrane pad 16, detection is with 17, quality control band 18, cellulose nitrate analyzing film 19, strong adsorptive pads 20, strip is installed sign paper and is signed T: detect band C: quality control band
Embodiment
Below in conjunction with Fig. 1-Fig. 6, better embodiment of the present invention is described.
The invention provides a kind of melamine detection kit, it comprises that plastic casing 1, melamine detection test strip 13 and one are attached to the sampling pipette 5 that is used to draw sample on the kit box body, when detecting.
As depicted in figs. 1 and 2, described plastic casing 1 directly upwards is divided into housing previous section 2 and housing aft section 3; Described housing previous section 2 its upper surfaces sink to form a groove 4, and the sampling pipette 5 that is used to draw sample during detection is joined and put in the groove, notch post plastic wrappage 6 in case kit in transit sampling pipette 5 drop; Described housing 1 aft section 3 comprises dismountable loam cake 7 and lower cover 8, radially has well 9, testing result display window 10 on the loam cake 7 successively and sign hole 11 is installed; Shown in Figure 2, lower cover 8 inner radial are provided with strip groove 12, and described melamine detection test strip 13 is radially chimeric to be installed in this strip groove 12.
As shown in Figure 3 and Figure 4, described strip 13 be provided with sample pad 14, glass fibre membrane pad 15 that mutual overlap joint pastes in turn, have detect with 16 and the cellulose nitrate analyzing film 18 of quality control band 17, strong adsorptive pads 19 and strip sign paper be installed signed 20.
The glass fibre membrane pad 15 of described strip is provided with the monoclonal antibody of anti-melamine of colloid gold label and the rabbit antibody of colloid gold label; Have detect with 16 and the cellulose nitrate analyzing film 18 of quality control band 17 on detection be with 16 to be provided with melamine antigen, quality control band 17 is provided with the goat-anti rabbit polyclonal antibody.
Be installed in the melamine detection test strip 13 in the lower cover internal recess 12 of plastic casing 1 aft section 3, sample pad 14 on it, have detect with 16 and the cellulose nitrate analyzing film 18 of quality control band 17 and strip sign paper be installed signed 20 respectively with well 9, the testing result display window 10 of plastic casing loam cake 7 with sign hole 11 corresponding placements are installed, can see strip installation sign paper label 20 by the installation sign hole 11 of plastic casing loam cake 7, whether correctly install to determine strip 13.
During sample detection, tear the plastic wrappage 6 that covers on the sampling pipette groove 4, take out sampling pipette 5, draw sample liquid to be checked with this sampling pipette 5, dropping 3-4 drips and treats corpse or other object for laboratory examination and chemical testing sample liquid in the sample pipetting volume hole 9 of described kit, the 3-5 minute testing result that can detect by an unaided eye.
Melamine detection kit test sample tripolycyanamide provided by the invention adopts competition law.
If do not contain tripolycyanamide in the sample, the anti-melamine monoclonal antibody gold mark thing that is provided with on the strip glass fibre membrane pad 15 oozes the detection that moves on to strip cellulose nitrate analyzing film when being with 16 positions with sample, anti-melamine monoclonal antibody on the colloidal gold particle will be arranged on the melamine antigen that strip cellulose nitrate analyzing film detects with 16 positions and combine, and assemble red zone of formation, this shows that testing result is negative.
If contain tripolycyanamide in the sample, the limited anti-melamine monoclonal antibody that they will compete colloid gold label on the strip with the set melamine antigen in 16 positions with the detection of strip cellulose nitrate analyzing film, and combine with it.When the concentration of the tripolycyanamide in the sample to be checked meets or exceeds when a certain amount of, tripolycyanamide in the sample will combine with the limited anti-melamine monoclonal antibody of strip colloid gold label, thereby make and be arranged on strip and detect with the melamine antigen on 16 and can't combine with the anti-melamine monoclonal antibody of colloid gold label, being provided with the detection of melamine antigen this moment is with 16 red zone can not occur, this shows in this sample and contains tripolycyanamide that testing result is positive.
The glass fibre membrane pad 15 of the strip of melamine detection kit provided by the invention is provided with the colloid gold label thing of rabbit antibody.This label together oozes when moving to quality control band 17 with sample, can react with the goat-anti rabbit polyclonal antibody that quality control band 17 is provided with (the colloid gold label thing of this rabbit antibody can not react with 16 melamine antigen that are provided with detection), quality control band 17 position display red zones also can be identified by naked eyes.When in the sample detection because certain constituent mass problem can't detect when test, strip is provided with quality control band 17 positions of goat-anti rabbit polyclonal antibody and then can develop the color, so, can be to sample melamine detection carrying out quality control by the Quality Control thing goat-anti rabbit polyclonal antibody that strip quality control band 17 is provided with.Quality control band 17 redfree bands show that testing result is invalid.
The present invention also provides a kind of preparation technology of melamine detection kit, and it may further comprise the steps:
A, handle sample pad 14 with closed reagent.The prescription of described closed reagent is: sodium taurocholate 0.1-0.7%, Tween-20 0.05-1%, polyethoxy oleyl alcohol 0.5-2%, sodium chloride 0.5-1.2%, pure water 95-99%, sodium phosphate (PB) damping fluid 0.01-0.03mmol/L;
B, configuration colloidal gold solution
Regulate the pH of colloidal gold solution to selected standard with the sal tartari of 0.2M or the hydrochloric acid of 0.1M; The monoclonal antibody of volume is an amount of, that concentration is suitable anti-melamine becomes colloidal gold solution with rabbit antibody rapid mixing; The colloidal gold solution point that is disposed is added on the glass fibre membrane, forms the glass fibre membrane pad 15 of colloid gold label;
C, configuration melamine antigen solution and configuration goat anti-rabbit antibody solution form detection with some film instrument to the cellulose nitrate analyzing film 18 with their speckings respectively separated by a distance then and are with 16 and quality control band 17;
D, respectively sample pad 14, point are added with the colloid gold label thing glass fibre membrane pad 15, have detect with 16 and the cellulose nitrate analyzing film 18 of quality control band 17, strong adsorptive pads 19 and strip install sign paper sign 20 successively mutually overlap joint paste on the offset plate of an adhesive sticker, cut into the strip of certain-length and width afterwards, and strip is installed in the groove 12 in the plastic casing aft section lower cover 8;
E, vacuum drying: the temperature in the vacuum pump is controlled at 15-25 ℃; The vacuum drying time was controlled at 2-5 hour; Atmospheric pressure in the vacuum pump is controlled at below 0.1 millibar (mba);
F, foil sealing packing are promptly finished a melamine detection kit and are made.
Melamine detection kit provided by the invention and preparation technology thereof have the following advantages:
1, described kit is simple in structure, easily makes, and is with low cost, is particularly suitable for suitability for industrialized production.
2, described kit is used for the test sample tripolycyanamide fast, accurately, and is easy and simple to handle, easy to carry.Detection suitablely all can be used without any need for instrument and equipment and reagent in good time.Operation does not need special study and training, and professional and technical personnel and ordinary person all are suitable for.During sample detection, operating personnel only need tear the plastic wrappage 6 of kit surface sample suction pipe 5 off, take out sample suction pipe 5, suction pipe 5 is directly used in draws sample to be checked and drip after sample to be checked goes into the well 9 of kit, just can obtain testing result in 3-5 minute.
3, reagent and the material in the described kit do not relate to active inactivation, can be in the room temperature long preservation.
4, described kit has good practicality and market popularization value, can be used for biological sample, especially the tripolycyanamide field quick detection and the extensive examination of dairy products in the food sanitation safe etc.
Fig. 5-Fig. 6 is the testing result synoptic diagram that kit of the present invention is used for detection of biological sample tripolycyanamide.Fig. 5 represents that No. 1 sample does not contain melamine; Fig. 6 represents that No. 2 samples contain melamine.
Special needs to be pointed out is; melamine detection kit of the present invention and preparation technology thereof can also have some improvement; for example; wherein the colloid gold label thing of melamine detection test strip is also replaceable is electroselenium label etc.; therefore; allly adopt any being equal to replace or other technical scheme that equivalent transformation forms, all drop in the protection domain of claim of the present invention melamine detection kit of the present invention and preparation technology thereof.
Claims (7)
1, a kind of melamine detection kit comprises plastic casing (1) and melamine detection test strip (13), it is characterized in that:
Described plastic casing (1) footpath upwards is divided into previous section (2) and aft section (3); Its upper surface of described previous section (2) sink and forms a groove (4), be furnished with the sampling pipette (5) that sample detection is used in the groove, notch with plastic wrappage (6) sampling pipette (5) is encapsulated in the groove (4) in case kit in transit sampling pipette (5) drop;
Described aft section (3) comprises dismountable loam cake (7) and lower cover (8); Radially have well (9), testing result display window (10) on the loam cake (7) of described aft section (3) successively and sign hole (11) is installed; Lower cover (8) inner radial of described aft section (3) is provided with strip groove (12); Described strip (13) is radially chimeric to be installed in this strip groove (12);
Described strip (13) is provided with sample pad (14), glass fibre membrane pad (15), the cellulose nitrate analyzing film (18) with detection band (16) and quality control band (17), strong adsorptive pads (19) and the strip installation sign paper label (20) that mutual overlap joint is pasted in turn.
2, melamine detection kit according to claim 1 is characterized in that: the glass fibre membrane pad (15) of described strip is provided with the monoclonal antibody of anti-melamine of colloid gold label and the rabbit antibody of colloid gold label.
3, melamine detection kit according to claim 1 is characterized in that: the quality control band of described strip (17) is provided with the goat-anti rabbit polyclonal antibody.
4, melamine detection kit according to claim 1 is characterized in that: the detection band (16) of described strip is provided with melamine antigen.
5, a kind of preparation technology of melamine detection kit may further comprise the steps:
A, use closed reagent are handled sample pad (14);
B, configuration colloidal gold solution: regulate the pH of colloidal gold solution to selected mark Huaihe River with the sal tartari of 0.2M or the hydrochloric acid of 0.1M; The monoclonal antibody of volume is an amount of, that concentration is suitable anti-melamine becomes colloidal gold solution with rabbit antibody rapid mixing; Described colloidal gold solution point is added on the glass fibre membrane, forms the glass fibre membrane pad (15) of colloid gold label;
C, configuration melamine antigen solution and configuration goat anti-rabbit antibody solution are gone up their speckings respectively separated by a distance to form to cellulose nitrate analyzing film (18) then and are detected band (16) and quality control band (17);
D, respectively sample pad (14), point are added with the colloid gold label thing glass fibre membrane pad (15), have the cellulose nitrate analyzing film (18) that detects band (16) and quality control band (17), strong adsorptive pads (19) and strip and sign paper is installed is signed (20) mutual successively overlap joint and paste on the offset plate of an adhesive sticker, cut into the strip of certain-length and width afterwards, and strip is installed in the interior groove (12) of plastic casing (1) aft section (3) lower cover (8);
E, carry out vacuum drying;
F, carry out foil sealing packing.
6, the preparation technology of melamine detection kit according to claim 5 is characterized in that: the prescription of described closed reagent is: sodium taurocholate 0.1-0.7%, Tween-20 0.05-1%, polyethoxy oleyl alcohol 0.5-2%, sodium chloride 0.5-1.2%, pure water 95-99%, sodium phosphate buffer 0.01-0.03mmol/L.
7, the preparation technology of melamine detection kit according to claim 5 is characterized in that: described vacuum drying condition is: temperature is at 15-25 ℃ in the vacuum pump, and the vacuum drying time, atmospheric pressure was below 0.1 millibar in the vacuum pump at 2-5 hour.
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| CN101403758B CN101403758B (en) | 2013-07-24 |
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Cited By (4)
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| CN101943698A (en) * | 2010-09-03 | 2011-01-12 | 山西大学 | Dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and preparation method thereof |
| CN102388310A (en) * | 2009-04-09 | 2012-03-21 | 爱科来株式会社 | Sample analyzing tool, method of manufacturing sample analyzing tool, and method of minimizing drop in solution permeability of deployment member |
| CN105393108A (en) * | 2013-04-30 | 2016-03-09 | 成都领御生物技术有限公司 | Quantum-dot immunochromatographic test strip detection system and use thereof |
| CN107219350A (en) * | 2017-05-31 | 2017-09-29 | 深圳市国赛生物技术有限公司 | A kind of kit |
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| CN1948965A (en) * | 2006-09-01 | 2007-04-18 | 河南省农业科学院生物技术研究所 | Gold chromatography detecting test paper strip of furanzoleone metabolite colloid and test paper card thereof |
| CN101183105B (en) * | 2007-12-13 | 2011-06-29 | 中国农业科学院农业质量标准与检测技术研究所 | ELISA reagent kit for detecting melamine |
| CN101206223B (en) * | 2007-12-13 | 2011-06-29 | 中国农业科学院农业质量标准与检测技术研究所 | Direct competition method ELISA reagent box for detecting melamine |
| CN201293785Y (en) * | 2008-10-20 | 2009-08-19 | 马义才 | Rapid detection kit for melamine |
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| CN102388310A (en) * | 2009-04-09 | 2012-03-21 | 爱科来株式会社 | Sample analyzing tool, method of manufacturing sample analyzing tool, and method of minimizing drop in solution permeability of deployment member |
| CN102388310B (en) * | 2009-04-09 | 2014-07-16 | 爱科来株式会社 | Sample analyzing tool, method of manufacturing sample analyzing tool, and method of minimizing drop in solution permeability of deployment member |
| US9903864B2 (en) | 2009-04-09 | 2018-02-27 | Arkray, Inc. | Sample analysis tool, method for producing sample analysis tool, and method for inhibiting decrease in liquid permeability of development member |
| CN101943698A (en) * | 2010-09-03 | 2011-01-12 | 山西大学 | Dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and preparation method thereof |
| CN105393108A (en) * | 2013-04-30 | 2016-03-09 | 成都领御生物技术有限公司 | Quantum-dot immunochromatographic test strip detection system and use thereof |
| CN107219350A (en) * | 2017-05-31 | 2017-09-29 | 深圳市国赛生物技术有限公司 | A kind of kit |
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