CN204556639U - Aflatoxins M1 immunochromatographydetecting detecting test strip - Google Patents
Aflatoxins M1 immunochromatographydetecting detecting test strip Download PDFInfo
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- CN204556639U CN204556639U CN201520275946.9U CN201520275946U CN204556639U CN 204556639 U CN204556639 U CN 204556639U CN 201520275946 U CN201520275946 U CN 201520275946U CN 204556639 U CN204556639 U CN 204556639U
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- aflatoxins
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Abstract
The utility model discloses a kind of Aflatoxins M1 immunochromatographydetecting detecting test strip, comprise Test paper card, also comprise gold mark micropore, described gold mark micropore separate with described Test paper card and arranges separately, and described golden micropore endoperidium of marking has Aflatoxins M1 monoclonal antibody-colloid gold label thing.Aflatoxins M1 immunochromatographydetecting detecting test strip of the present utility model, adopt gold mark micropore to separate with described Test paper card to arrange separately, detected sample and gold is first allowed to mark after micropore reacts, add again in Test paper card and develop the color, sample and reactant liquor can be made to react more abundant, avoid that detected sample directly to be added the reaction of Test paper card insufficient, detect inaccurate problem; And do not need to re-use other to detect, simple to operate.
Description
Technical field
The utility model belongs to biological technical field, relates to a kind of Test paper card, is specifically related to a kind of immunochromatographytest test kit, particularly relates to a kind of Aflatoxins M1 immunochromatographydetecting detecting test strip.
Background technology
Aflatoxin is a kind of strong carcinogenic substance, and be the mushrooms such as Aspergillus height poison for thing.All there are strict regulation limitations in most of government organs to the aflatoxin amount that human body and animal can be taken in.There has been clear and definite limit standard in a lot of country to the Aflatoxins M1 content in milk and dairy products.It is 1 class carcinogenic substance that aflatoxin delimited by the Agency for Research on Cancer of the World Health Organization (WHO) (WHO) for 1993.
Aflatoxins M1 belongs to the one in the compound similar to aflatoxin structure, occurs that the probability of aflatoxin is the highest in the food and feed of damp-heat area.Physicochemical property quite stable, is not destroyed by pasteurization.Mammal changes into Aflatoxins M1 by hydroxylation after taking in the feed or food polluted by aflatoxin B1.Aflatoxins M1 harm is mainly manifested in carcinogenicity and mutagenicity, has destruction, liver cancer can be caused even dead to people and animal's liver tissue.
In European Union, the Aflatoxins M1 content in milk and milk powder is limited in 0.05mg/L or 50ppt.In China, the mxm. of Aflatoxins M1 national regulation is 0.5 μ g/kg.
At present, thin-layered chromatography, high performance liquid chromatography, immunoassay, mass spectroscopy, immune affine fluorimetry etc. are mainly contained to the method that aflatoxin detects.Thin-layered chromatography complex operation, pollute greatly, quantitatively poor, consuming time length; High performance liquid chromatography has highly sensitive, the feature such as separating power is strong, specificity good and measurement result is reliable, if but food composition is complicated, before carrying out liquid chromatography separation, thoroughly effective purified treatment need be made to sample, be not suitable for the detection of batch samples, application is restricted; Enzyme linked immunosorbent assay is a kind of detection method of normal use at present, it have quick, sensitive, to the advantage such as sample purity is less demanding, be specially adapted to the detection of batch samples, but owing to needing the personnel of microplate reader and skilled operation, be not suitable for field quick detection.
Detect in Aflatoxins M1 quick, easy, practical at present, have only a few test strip, and there is detection not exclusively, the problems such as detection efficiency is low, and producing cost is high.
Summary of the invention
For solving the problems of the technologies described above, the purpose of this utility model is to provide a kind of Aflatoxins M1 immunochromatographydetecting detecting test strip, thus can detect more accurate, and do not need other reagent, simple to operate, cost is low.
For achieving the above object, the technical solution of the utility model is as follows:
On the one hand, the utility model provides a kind of Aflatoxins M1 immunochromatographydetecting detecting test strip, comprise Test paper card, also comprise gold mark micropore, described gold mark micropore separates with described Test paper card and arranges separately, described gold mark micropore endoperidium has Aflatoxins M1 monoclonal antibody-colloid gold label thing, and described gold mark micropore is used for obtaining reactant liquor with detection example reaction, is then added in Test paper card by reactant liquor and develops the color.
Aflatoxins M1 immunochromatographydetecting detecting test strip of the present utility model, adopt gold mark micropore to separate with described Test paper card to arrange separately, detected sample and gold is first allowed to mark after micropore reacts, add again in Test paper card and develop the color, sample and reactant liquor can be made to react more abundant, avoid that detected sample directly to be added the reaction of Test paper card insufficient, detect inaccurate problem; And do not need to re-use other to detect.
On the basis of technique scheme, the utility model also can make following improvement:
As preferred scheme, in described gold mark micropore, also comprise reagent.
Adopt above-mentioned preferred scheme, in gold mark micropore, add detection reagent, do not need additionally to add other again and detect reagent, accurately can control the amount detecting reagent, directly use during use, simple to operate, accuracy rate is high.
As preferred scheme, described Test paper card comprises base and cover, and described cover is fastened on base, described base is attached with test paper core, and described test paper core comprises the sample pad, coated film and the adsorptive pads that set gradually; Described cover is provided with well and view window, and described well, view window are corresponding with described sample pad and coated film respectively.
As preferred scheme, described cover is also provided with detection line trace " C " and nature controlling line trace " T ".
As preferred scheme, described base is also provided with the first groove, described test paper core is attached in described first groove.
Adopt above-mentioned preferred scheme, by test paper core is attached on the first groove, added example reaction liquid can be made to store in a groove, avoid adding reactant liquor, reactant liquor overflow can also be avoided, thus make detection more accuracy.
As preferred scheme, described first groove side is also provided with the second groove, and described second groove is connected with the first groove by diversion trench.
Adopt above-mentioned preferred scheme, by the setting of the second groove, unnecessary reactant liquor can be made to flow into the second groove by diversion trench from the first groove, avoid reactant liquor to flow to view window, the accuracy of impact reaction.
As preferred scheme, described cover is also provided with the support column be resisted against in sample pad.
Adopt above-mentioned preferred scheme, by support column against sample pad, test paper core can be made to contact with reactant liquor more fully, thus make detection more accurate.
Accompanying drawing explanation
Fig. 1 is the front view of the Test paper card in Aflatoxins M1 immunochromatographydetecting detecting test strip of the present utility model;
Fig. 2 is that Test paper card in Aflatoxins M1 immunochromatographydetecting detecting test strip of the present utility model removes one of front view after cover;
Fig. 3 is the cut-open view of the Test paper card in Fig. 1;
Fig. 4 is the front view two after Test paper card in Aflatoxins M1 immunochromatographydetecting detecting test strip of the present utility model removes cover;
Fig. 5 is the structural representation of the gold mark micropore in Aflatoxins M1 immunochromatographydetecting detecting test strip of the present utility model;
Wherein: 1. Test paper card, 2. gold mark micropore, 3. base, 4. cover, 5. sample pad, 6. coated film, 7. adsorptive pads, 8. well, 9. view window, 10. detection line, 11. nature controlling lines, 12. first grooves, 13. second grooves, 14, diversion trench, 15. support columns.
Embodiment
Preferred implementation of the present utility model is described in detail below in conjunction with accompanying drawing.
In order to reach the purpose of this utility model, in some embodiments, as shown in Fig. 1-3 or 5, Aflatoxins M1 immunochromatographydetecting detecting test strip of the present utility model, comprise Test paper card 1, also comprise gold mark micropore 2, described gold mark micropore 2 separates with described Test paper card 1 and arranges separately, described gold mark micropore 2 endoperidium has Aflatoxins M1 monoclonal antibody-colloid gold label thing, reactant liquor, for obtaining reactant liquor with detection example reaction, then adds in Test paper card 1 and develops the color by described gold mark micropore 2.This Aflatoxins M1 immunochromatographydetecting detecting test strip, adopt gold mark micropore to separate with described Test paper card to arrange separately, detected sample and gold is first allowed to mark after micropore reacts, add again in Test paper card and develop the color, sample and reactant liquor can be made to react more abundant, avoid that detected sample directly to be added the reaction of Test paper card insufficient, detect inaccurate problem; And do not need to re-use other to detect.
In order to optimize implementation result of the present utility model further, in described gold mark micropore 2, also comprise reagent.By adding detection reagent in gold mark micropore, do not need additionally to add other again and detect reagent, accurately can control the amount detecting reagent, directly use during use, simple to operate, accuracy rate is high.
In order to optimize implementation result of the present utility model further, described Test paper card 1 comprises base 3 and cover 4, described cover 4 fastens on the base 3, and described base 3 is attached with test paper core, and described test paper core comprises the sample pad 5, coated film 6 and the adsorptive pads 7 that set gradually; Described cover 4 is provided with well 8 and view window 9, and described well 8, view window 9 are corresponding with sample pad 5 and coated film 6 respectively.
In order to optimize implementation result of the present utility model further, described cover 4 is also provided with detection line 10 trace " C " and nature controlling line trace 11 " T ".
In order to optimize implementation result of the present utility model further, described cover 4 is also provided with the support column 15 be resisted against in sample pad 5.By support column against sample pad, test paper core can be made to contact with reactant liquor more fully, thus make detection more accurate.
In order to optimize implementation result of the present utility model further, in other embodiment, as shown in Figure 4, described base 3 is also provided with the first groove 12, described test paper core is attached in described first groove 12.By test paper core is attached on the first groove, added example reaction liquid can be made to store in a groove, avoid adding reactant liquor, reactant liquor overflow can also be avoided, thus make detection more accuracy.
In order to optimize implementation result of the present utility model further, described first groove 12 side is also provided with the second groove 13, and described second groove 13 is connected with the first groove 12 by diversion trench 14.By the setting of the second groove, unnecessary reactant liquor can be made to flow into the second groove by diversion trench from the first groove, avoid reactant liquor to flow to view window, the accuracy of impact reaction.
During use, operate as follows:
1. tear Aflatoxins M1 immunochromatographydetecting detecting test strip packaging, take out gold mark micropore.(temperature of experimental situation must more than 20 DEG C, the former milk of freezing mistake, obviously occur easily causing running plate incomplete by particle, well heater now must be used to heat or centrifuging and taking intermediate sample detects);
2. get 120 μ l (or get 3-4 with dropper drip) lactogenesis in gold mark micropore with liquid-transfering gun, with dropper, the gold in micropore and lactogenesis are fully mixed after 2 minutes, after waiting 2 minutes again, the lactogenesis dropper of mixing is all drawn in the well being added drop-wise to Test paper card.
3. sentence read result after keeping flat standing 5-10 minute; After 30 minutes, result interpretation is invalid.
4. result intuitive display, accurate, simple and clear.The testing result of Test paper card is all using brownish red lines " | " and " || " negative and positive interpretation as testing result, namely when area of observation coverage detection line " C " shows a reddish brown colo(u)r streak " | ", nature controlling line " T " does not develop the color, represent that in sample, Aflatoxins M1 exceeds standard, if show a reddish brown colo(u)r streak " | " at area of observation coverage detection line " C " and nature controlling line " T " simultaneously, show that in sample, Aflatoxins M1 does not exceed standard.
Above-described is only preferred implementation of the present utility model; it should be pointed out that for the person of ordinary skill of the art, under the prerequisite not departing from the utility model creation design; can also make some distortion and improvement, these all belong to protection domain of the present utility model.
Claims (7)
1. an Aflatoxins M1 immunochromatographydetecting detecting test strip, comprise Test paper card, it is characterized in that, also comprise gold mark micropore, described gold mark micropore separate with described Test paper card and arranges separately, and described golden micropore endoperidium of marking has Aflatoxins M1 monoclonal antibody-colloid gold label thing.
2. Aflatoxins M1 immunochromatographydetecting detecting test strip according to claim 1, is characterized in that, also comprises reagent in described gold mark micropore.
3. Aflatoxins M1 immunochromatographydetecting detecting test strip according to claim 1 and 2, it is characterized in that, described Test paper card comprises base and cover, described cover is fastened on base, described base is attached with test paper core, described test paper core comprises the sample pad, coated film and the adsorptive pads that set gradually; Described cover is provided with well and view window, and described well, view window are corresponding with described sample pad and coated film respectively.
4. Aflatoxins M1 immunochromatographydetecting detecting test strip according to claim 3, is characterized in that, described cover is also provided with detection line trace " C " and nature controlling line trace " T ".
5. Aflatoxins M1 immunochromatographydetecting detecting test strip according to claim 3, is characterized in that, described base is also provided with the first groove, and described test paper core is attached in described first groove.
6. Aflatoxins M1 immunochromatographydetecting detecting test strip according to claim 5, is characterized in that, described first groove side is also provided with the second groove, and described second groove is connected with the first groove by diversion trench.
7. Aflatoxins M1 immunochromatographydetecting detecting test strip according to claim 3, is characterized in that, described cover is also provided with the support column be resisted against in sample pad.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520275946.9U CN204556639U (en) | 2015-04-30 | 2015-04-30 | Aflatoxins M1 immunochromatographydetecting detecting test strip |
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| CN201520275946.9U CN204556639U (en) | 2015-04-30 | 2015-04-30 | Aflatoxins M1 immunochromatographydetecting detecting test strip |
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| CN204556639U true CN204556639U (en) | 2015-08-12 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198966A (en) * | 2016-09-06 | 2016-12-07 | 中国农业大学 | A kind of near-infrared fluorescent immune chromatography reagent kit detecting aflatoxin and application thereof |
| CN107064496A (en) * | 2017-06-05 | 2017-08-18 | 苏州快捷康生物技术有限公司 | A kind of malachite green colloidal gold immunochromatographimethod rapid detection card and preparation method thereof |
| CN112881696A (en) * | 2021-01-13 | 2021-06-01 | 北京中检葆泰生物技术有限公司 | Detection method and system for stably detecting aflatoxin content |
-
2015
- 2015-04-30 CN CN201520275946.9U patent/CN204556639U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198966A (en) * | 2016-09-06 | 2016-12-07 | 中国农业大学 | A kind of near-infrared fluorescent immune chromatography reagent kit detecting aflatoxin and application thereof |
| CN107064496A (en) * | 2017-06-05 | 2017-08-18 | 苏州快捷康生物技术有限公司 | A kind of malachite green colloidal gold immunochromatographimethod rapid detection card and preparation method thereof |
| CN112881696A (en) * | 2021-01-13 | 2021-06-01 | 北京中检葆泰生物技术有限公司 | Detection method and system for stably detecting aflatoxin content |
| CN112881696B (en) * | 2021-01-13 | 2021-11-02 | 北京中检葆泰生物技术有限公司 | Detection method and system for stably detecting aflatoxin content |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150812 Termination date: 20200430 |