CN102507880A - Card for quickly detecting acrylamide, and detection method therefor - Google Patents
Card for quickly detecting acrylamide, and detection method therefor Download PDFInfo
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- CN102507880A CN102507880A CN2011103497690A CN201110349769A CN102507880A CN 102507880 A CN102507880 A CN 102507880A CN 2011103497690 A CN2011103497690 A CN 2011103497690A CN 201110349769 A CN201110349769 A CN 201110349769A CN 102507880 A CN102507880 A CN 102507880A
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Abstract
The invention discloses a card for quickly detecting acrylamide, and a detection method therefor, and belongs to the technical field of detection of the acrylamide. A test strip is arranged in a shell of the card for quickly detecting the acrylamide, and is formed by sequentially adhering a sample cushion, a colloidal gold (or latex particle) membrane, a nitrocellulose membrane and a water absorption membrane to a support back plate; the colloidal gold (or latex particle) membrane is a glass fiber membrane containing anti-acrylamide monoclonal antibody colloidal gold (or anti-acrylamide monoclonal antibody latex particle) markers; and two detection strips, namely a detection strip containing acrylamide artificial antigen and a quality control strip containing anti-rabbit antibody or anti-mouse antibody are arranged on the nitrocellulose membrane in a horizontal direction. The invention has the advantages that the content of the acrylamide in a food sample is directly detected by an immunological method. The card for quickly detecting the acrylamide is easy to manufacture, can be used conveniently and quickly and is accurate in result.
Description
Technical field
The present invention relates to a kind of utensil that detects acrylic amide, particularly relate to a kind of rapid detection card and detection method thereof of acrylic amide.
Background technology
In April, 2002; Stockholm Univ Sweden and Sweden state food management review personnel take the lead in reporting; From fries, potato chips; Detected high concentration suspect carcinogen-acrylic amide (acrylamide) in the food such as biscuit, many subsequently countries institute an inquiry one after another, have all obtained the result similar with Sweden.In February, 2005, the FAO (Food and Agriculture Organization of the United Nation) (FAO) and the combination food adjuvant Committee of Experts of The World Health Organization (WHO) (JECFA) the 64th meeting carried out the risk assessment of system according to over the past two years new data to the acrylic amide in the food.
Acrylic amide is a kind of white crystal appearance material; Relative molecular mass is 71.08; Stable, soluble in water under the room temperature, in ethanol, ether, acetone and other organic solvent, be prone to polymerization and copolymerization. acrylic amide is the raw material of industry of producing polyacrylamide; And be widely used in the synthetic of dyestuff, plastic cement and be used as the thickening agent in building materials and the commodity, and polyacrylamide is used for sewage and drinking water treatment as coagulant aid.The pollution of acrylic amide is relevant with high temperature process in the food, and research shows: the high foods of starch-containing carbohydrates such as bread, fries, potato chips, through high temperature more than 120 ℃ toast for a long time, fried, can detect acrylic amide.Zoopery proof acrylic amide has nerve, reproduction, genetoxic and potential carcinogenicity.Based on its cumulative effect, long term exposure is especially eaten the absorption of source property in the acrylic amide of low concentration there is potential hazard in body, at present, in human body, has detected acrylic amide dna adduct and protein adduct.Find according to China's investigation; Acrylic amide content in potato class fried food is 0.78-3.21mg/kg; Investigation simultaneously finds that the Beijing area has resident's acrylic amide intake of 67.8% to surpass acceptable intake every day of U.S. FDA; And tangible age differences is arranged, and the exposure level of children's acrylic amide is 2-3 a times of adult, so the harm of acrylic amide should cause people's extensive concern in the food.
The present invention sends out bright a kind of based on having antigenic immunological detection method after the small-molecule substance acrylic amide coupling protein matter; Take immunology competition ratio juris; A kind of quick detection reagent of association colloid gold (or latex particle) labelling technique and immunochromatographic method design can detect the acrylic amide in the food fast specifically.Reaction is to utilize the protein conjugate antigenicity of the similar thing of acrylic amide and a kind of detection method of setting up, has the advantage of high specificity, the flase drop problem that can avoid the false positive of other detection method to cause.Test card is simple to operate simultaneously need not special instrument and equipment fast, is fit to execute-in-place.Because sample size needs less, easy fast, be applicable to the examination of great amount of samples.The result is accurate, and is highly sensitive, and accuracy rate is big.
Summary of the invention
The object of the present invention is to provide a kind of appliance that can detect acrylamide content in the food; Directly the method for applied immunology detects the content of acrylic amide in the food; Overcome the shortcomings such as complex operation that existing chromatography detects, set up based on the specific detection method of immunology.
Technical scheme of the present invention: a kind of acrylic amide rapid detection card, it is for being provided with test-strips 2 in the test card shell 1 of rectangular flat shell shape, and the test card case surface has fenestra of detection 3 and well 4; Test-strips 2 is formed by pasting sample pad 8, collaurum (or latex particle) film 7, nitrocellulose filter 6 and 9 of absorbing membranes above that successively through adhesive sticker on the supporting backboard 5; The stacked stickup nitrocellulose filter 6 in supporting backboard middle part; One endlap of supporting backboard is put and is pasted absorbing membrane 9; The stacked stickup sample pad 8 of the other end; Absorbing membrane is inner to overlap with nitrocellulose filter respectively with sample pad the inner separately, at the clinch of sample pad and nitrocellulose filter, inserts and puts between the two and pastes one section collaurum (or latex particle) film 7; Collaurum (or latex particle) film is for containing the glass fibre membrane of anti-acrylic amide antibody colloidal gold (or latex particle) mark; There is one to detect band and a quality control band on the nitrocellulose filter; Be successively: the detection that contains N-acryloxy succinimide protein conjugate is with 10, contains the quality control band 11 of anti-rabbit antibody or anti-mouse antibody, and test-strips is inserted in the test card shell; Sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
Adopt aforesaid propylene acid amides rapid detection card, detect the content of acrylic amide in the food, the disposal route of test sample is following:
A: if sample is solution, can directly detect or simple filtration after detect
B:, behind 1% aqueous formic acid stirring at room 2h, get filtered fluid and detect if sample is a solid.
When detection be with 10 with quality control band 11 manifest brownish red trace band simultaneously, testing result is negative, representes that acrylamide content does not exceed standard in the sample to be detected.
Be with 10 Show Colors not when detection, have only quality control band 11 to manifest the brownish red trace, testing result is positive, representes that acrylamide content exceeds standard in the sample to be detected.
Be with 10 all not develop the color with quality control band 11 when detection, show that then test-strips lost efficacy.
Beneficial effect of the present invention: advantage of the present invention is to detect acrylamide content in the food, is easy to preparation and saves testing cost, and is easy to use, detects fast, and highly sensitive, the result is accurate.
Description of drawings
Fig. 1 acrylic amide rapid detection card shell and test-strips arrangement figure in the enclosure.1, shell, 2, test-strips, 3, detect fenestra, 4, well.
Show the trace synoptic diagram on the nitrocellulose filter of Fig. 2 test-strips.6, nitrocellulose filter, 10, detect band, 11, quality control band.
The test-strips structural representation of Fig. 3 acrylic amide rapid detection card.5, supporting backboard, 6, nitrocellulose filter, 7, collaurum (or latex particle) film, 8, sample pad, 9, absorbing membrane.
Embodiment
The present invention combines specific embodiment to further specify as follows referring to accompanying drawing:
Embodiment 1
A kind of acrylic amide rapid detection card, it is that test-strips 2 is set in test card shell 1, the setting of the structure of test card shell 1 and test-strips 2 is referring to accompanying drawing 1; Test card shell 1 is rectangular flat shell shape shell, long 70mm, wide 20mm; Thick 5mm, shell wall thickness 1mm is processed by engineering plastics.Test card shell 1 is connected with the shell block two halves by cap and constitutes, and fenestra of detection 3 and well 4 are arranged on test card shell 1 cap.Test-strips 2 is placed in the shell block of test card shell 1.
Test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 60mm, the wide about 4mm of sheet, the about 2.5mm of thickness.Test-strips 2 is sandwich constructions: bottom is a supporting backboard 5, is the igelite thin slice, thick about 0.5mm, long 60mm, wide 4mm; The stacked stickup nitrocellulose filter 6 in supporting backboard 5 middle parts, cellulose nitrate thickness 0.5mm, long 20-25mm, wide 4mm.Two isolated horizontal demonstration trace bands are arranged on the nitrocellulose filter 6, show in the trace band referring to 2, two in accompanying drawing, one is to detect to be with 10, contains the protein conjugate of N-acryloxy succinimide; Another is a quality control band 11, contains anti-rabbit antibody or anti-mouse antibody.Stacked stickup absorbing membrane 9 on supporting backboard 5 one ends; Stacked stickup sample pad 8 on supporting backboard 5 the other end; Absorbing membrane 9 the inners overlap with nitrocellulose filter 6 one ends respectively with sample pad 8 the inners separately; At the clinch of sample pad 8 with nitrocellulose filter 6, insert and put between the two and paste one section collaurum (or latex particle) film 7, collaurum (or latex particle) film 7 is the glass fibre membranes that contain anti-acrylic amide monoclonal antibody collaurum (or latex particle) label.Collaurum (or latex particle) film 7 thick 0.5-0.8mm, long 5mm, wide 4mm.Sample pad 8 thick 0.5-0.8mm, long 20mm, wide 4mm.Absorbing membrane 9 thick 0.5-0.8mm, long 16-20mm, wide 4mm.Collaurum (or latex particle) film 7, sample pad 8 and absorbing membrane 9 are 1-2mm with the nitrocellulose filter lap of splice.Absorbing membrane 9 materials are thieving paper, and sample pad 8 materials are water adsorption glass tunica fibrosas.
Acrylic amide test card of the present invention is used for the detection of food acrylic amide.
Embodiment 2
The disposal route of sample is following:
A: if sample is solution, can directly detect or simple filtration after detect
B:, behind 1% aqueous formic acid stirring at room 2h, get filtered fluid and detect if sample is a solid.
When using the acrylic amide rapid detection card to detect; Earlier sample liquid to be detected is instilled into from well 4 on the sample pad 8 of acrylic amide rapid detection card; Because syphonic effect principle; Drive contained anti-acrylic amide monoclonal antibody collaurum (or latex particle) labels of sample liquid to be detected and collaurum (or latex particle) film 7 together to nitrocellulose filter 6 diffusions, observations in 5-10 minute.
The key reaction of test card is immunologic antigen and antibody response; The antibody of the collaurum that on nitrocellulose filter, moves (or latex particle) mark; On calibration tape respectively with the protein conjugate that contains N-acryloxy succinimide; And anti-rabbit antibody of quality control band or anti-mouse antibody reaction, form the brownish red band.If have acrylic amide to be checked to exist in the sample, after sample adds, promptly react, and can not react, thereby do not develop the color with the protein conjugate of detection with contained N-acryloxy succinimide with antibody.The result of key reaction has following 3 kinds of situation:
1, when detection be with 10 and quality control band 11 manifest brownish red trace band simultaneously, the expression testing result negative, explain that acrylamide content does not exceed standard in the sample to be detected.
2, be with 10 Show Colors not when detection, have only quality control band 11 to manifest the brownish red trace, testing result is positive, explains that acrylamide content exceeds standard in the sample to be detected.
3, when detection be with 10 and quality control band 12 all do not develop the color, show that then detecting strip lost efficacy.
Claims (2)
1. acrylic amide rapid detection card, it is characterized in that: in the test card shell (1) of rectangular flat shell shape, test-strips (2) is set, the test card case surface has detection fenestra (3) and well (4); Test-strips (2) is upward pasted sample pad (8), collaurum film or latex particle film (7), nitrocellulose filter (6) and absorbing membrane (9) above that successively through adhesive sticker by supporting backboard (5) and is formed; Supporting backboard middle part stacked stickup nitrocellulose filter (6); One endlap of supporting backboard is put and is pasted absorbing membrane (9); The stacked stickup sample pad of the other end (8); Absorbing membrane is inner to overlap with nitrocellulose filter respectively with sample pad the inner separately; At the clinch of sample pad and nitrocellulose filter, insert and put between the two and paste one section collaurum film or latex particle film (7), the collaurum film is that the glass fibre membrane or the latex particle film that contain anti-acrylic amide antibody colloidal gold mark are the glass fibre membrane that contains anti-acrylic amide antibody latex particle mark; There is one to detect band and a quality control band on the nitrocellulose filter; Be successively: the detection band (10) that contains N-acryloxy succinimide protein conjugate; The quality control band (11) that contains anti-rabbit antibody or anti-mouse antibody; Test-strips is inserted in the test card shell, and sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
2. with the detection method of the described acrylic amide rapid detection card of claim 1, it is characterized in that the method for applied immunology directly detects the content of acrylic amide in the food, the disposal route of test sample is following:
A: sample is a solution, directly detects after detection or the simple filtration;
Or B: sample is a solid, behind the aqueous formic acid stirring at room 2h with mass concentration 1%, gets filtered fluid and detects;
When detection be with 10 with quality control band 11 manifest brownish red trace band simultaneously, testing result is negative, representes that acrylamide content does not exceed standard in the sample to be detected;
Be with 10 Show Colors not when detection, have only quality control band 11 to manifest the brownish red trace, testing result is positive, representes that acrylamide content exceeds standard in the sample to be detected;
Be with 10 all not develop the color with quality control band 11 when detection, show that then test-strips lost efficacy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103497690A CN102507880A (en) | 2011-11-08 | 2011-11-08 | Card for quickly detecting acrylamide, and detection method therefor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103497690A CN102507880A (en) | 2011-11-08 | 2011-11-08 | Card for quickly detecting acrylamide, and detection method therefor |
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| CN102507880A true CN102507880A (en) | 2012-06-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011103497690A Pending CN102507880A (en) | 2011-11-08 | 2011-11-08 | Card for quickly detecting acrylamide, and detection method therefor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103389235A (en) * | 2013-07-30 | 2013-11-13 | 欧陆分析技术服务(苏州)有限公司 | Extracting method of acrylamide test sample in roasted food |
| CN110441508A (en) * | 2019-05-06 | 2019-11-12 | 华南理工大学 | Acrylamide in Foods Rapid detection test strip based on nanogold composite coating |
| EP3221698B1 (en) * | 2014-11-20 | 2021-02-17 | Global Life Sciences Solutions Operations UK Ltd | Porous membranes with a polymer grafting, methods and uses thereof |
| CN112457407A (en) * | 2020-12-09 | 2021-03-09 | 华南农业大学 | Preparation and application of acrylamide specific nano antibody |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101285836A (en) * | 2007-04-10 | 2008-10-15 | 北京大学 | Acrylic amide complete antigen preparation and its ELISA quantitative determination method |
| CN102095857A (en) * | 2011-01-19 | 2011-06-15 | 无锡安迪生物工程有限公司 | Sodium vedproate rapid detection card and detecting method thereof |
-
2011
- 2011-11-08 CN CN2011103497690A patent/CN102507880A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101285836A (en) * | 2007-04-10 | 2008-10-15 | 北京大学 | Acrylic amide complete antigen preparation and its ELISA quantitative determination method |
| CN102095857A (en) * | 2011-01-19 | 2011-06-15 | 无锡安迪生物工程有限公司 | Sodium vedproate rapid detection card and detecting method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张齐等: "食品中丙烯酰胺分析的样品前处理技术", 《食品科技》 * |
| 黎其万等: "农药残留免疫分析方法及其应用研究进展", 《西南农业学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103389235A (en) * | 2013-07-30 | 2013-11-13 | 欧陆分析技术服务(苏州)有限公司 | Extracting method of acrylamide test sample in roasted food |
| EP3221698B1 (en) * | 2014-11-20 | 2021-02-17 | Global Life Sciences Solutions Operations UK Ltd | Porous membranes with a polymer grafting, methods and uses thereof |
| CN110441508A (en) * | 2019-05-06 | 2019-11-12 | 华南理工大学 | Acrylamide in Foods Rapid detection test strip based on nanogold composite coating |
| CN110441508B (en) * | 2019-05-06 | 2020-11-24 | 华南理工大学 | Test strip for rapidly detecting acrylamide in food based on nanogold composite coating |
| CN112457407A (en) * | 2020-12-09 | 2021-03-09 | 华南农业大学 | Preparation and application of acrylamide specific nano antibody |
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Application publication date: 20120620 |