CN102162814A - Quick detection card for acid orange II and detection method thereof - Google Patents
Quick detection card for acid orange II and detection method thereof Download PDFInfo
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- CN102162814A CN102162814A CN2011101411445A CN201110141144A CN102162814A CN 102162814 A CN102162814 A CN 102162814A CN 2011101411445 A CN2011101411445 A CN 2011101411445A CN 201110141144 A CN201110141144 A CN 201110141144A CN 102162814 A CN102162814 A CN 102162814A
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Abstract
The invention discloses a quick detection card for acid orange II and a detection method thereof, belonging to the technical field of non-edible materials illegally added in food. The quick detection card for acid orange II is characterized in that a shell is provided with a test strip, and a support back plate is successively stuck with a sample pad, a colloidal gold film or a latex particle film, a nitrocellulose film and a water adsorption film to form the quick detection card; the colloidal gold film is a glass fiber film labelled by an antibody colloidal gold containing an acid orange II resistant antibody, or the latex particle film is the glass fiber film labelled by latex particles containing an acid orange II resistant antibody; and the nitrocellulose film is provided with two detection bands, wherein one detection band contains an acid orange II protein conjugate, and the other detection band is a quality control band containing an anti-rabbit antibody or an anti-rat antibody. The quick detection method has the advantage that the content of the acid orange II in samples, such as marinating cooked foods, Capsicum frutescens, beverages and the like, can be directly detected with the immunology method. The quick detection card for acid orange II disclosed by the invention is easy to prepare, is convenient and quick to use and has an accurate result.
Description
Technical field
The present invention relates to a kind of Acid Orange II rapid detection card and detection method thereof, is a kind of appliance of detection of acidic orange II, belongs to the non-edible material from soybeans detection technique of illegal interpolation field in the food.
Background technology
Acid Orange II is the acid industrial dye of a kind of azo class, has another name called acid golden yellow II, gold orange II or acid gorgeous orange GR, the golden yellow powder of popular name.Water-soluble, ethanol.Be mainly used in the dyeing of silk, wool, also can dye leather, paper, polyamide fibre, the energy stamp also can be used as indicator.Dyeing, good level-dyeing property, dyefastness is relatively poor, is applicable to the knitting wool dyeing that colour brightness is high.It is red dark to meet the copper ion trend during dyeing, and chance ferric ion lighter color is dark.
The no any direct carcinogenesis of azo dyes itself, but reduction decomposition in vivo, can form aromatic amine compound, aromatic amine is in vivo through making DNA change with the target cell effect behind the metabolic activity, become the risk factor of human lesion, human body and animal are had potential carcinogenicity and mutagenicity.Because its carcinogenicity and mutagenicity, a lot of in the world countries have implemented strict regulation to the use of Acid Orange II.In China, Acid Orange II forbids being used as food additives.
Acid Orange II belongs to non-food coloring, does not allow in the food to add.But because Acid Orange II lovely luster, characteristics such as coloring stabilized, inexpensive, some illegal retailers use it for food production and processing in order to seek exorbitant profit, the preserved fruit that China is commercially available and outlet is overseas, bean product, spiced and stewed food, paprika, red shell melon seeds etc. all were detected similar synthetic non-food colorings such as containing Acid Orange II, serious harm the consumer healthy, and usually cause export food to be forced to destroy on the spot.So the exploitation of Acid Orange II detection method is very urgent.
At present, the main method of the Acid Orange II of illegal interpolation has high performance liquid chromatography and LC-MS etc. to carry out qualitative and quantitative analysis in the detection food.These methods require high to experimental instrument and equipment, complex operation is high to operating personnel's professional knowledge requirement, wastes time and energy.Therefore setting up a kind of analytical approach that detects the Acid Orange II of illegal interpolation in the food accurately and reliably again quickly and easily is very important.
The present invention sends out bright a kind of based on having antigenic immunological detection method after the small-molecule substance Acid Orange II coupling protein matter, take immunology competition ratio juris, a kind of quick detection reagent of association colloid gold or latex particle labelling technique and immunochromatographic method design can detect the Acid Orange II in the samples such as stew in soy sauce prepared food, paprika, beverage fast specifically.Acid orange II content in the samples such as stew in soy sauce prepared food, paprika, beverage is judged in the colour developing that has or not according to test card sample detection line and nature controlling line.Reaction is to utilize Acid Orange II protein conjugate antigenicity and a kind of detection method of setting up, has the advantage of high specificity, the flase drop problem that can avoid the false positive of other detection method to cause.Simultaneously test card is simple to operate need not special instrument and equipment fast, both can carry out in the laboratory, also can be in the supermarket, family, workshop etc. measure on the spot.Because sample size needs less, easy fast, be applicable to the examination of great amount of samples.The result is accurate, and is highly sensitive, and accuracy rate is big.
Summary of the invention
The object of the present invention is to provide a kind of appliance of energy detection of acidic orange II, directly the method for applied immunology detects the content of Acid Orange II in the samples such as stew in soy sauce prepared food, paprika, beverage, overcome the shortcomings such as complex operation that existing chromatography detects, set up based on the specific detection method of immunology.
Technical scheme of the present invention: a kind of Acid Orange II rapid detection card, it is for being provided with test-strips 2 in the test card shell 1 of rectangular flat shell shape, and the test card case surface has the fenestra 3 of detection and well 4; Test-strips 2 is formed by pasting sample pad 8, collaurum film or latex particle film 7, nitrocellulose filter 6 and absorbing membrane 9 thereon successively by adhesive sticker on the supporting backboard 5; The stacked stickup nitrocellulose filter 6 in supporting backboard middle part, one endlap of supporting backboard is put and is pasted absorbing membrane 9, the stacked stickup sample pad 8 of the other end, absorbing membrane is inner to overlap with nitrocellulose filter respectively separately with sample pad the inner, at the clinch of sample pad and nitrocellulose filter, insert and put between the two and paste one section collaurum film or latex particle film 7; The collaurum film is the glass fibre membrane that contains resistance to acid orange II antibody colloidal gold mark, or the latex particle film is the glass fibre membrane that contains resistance to acid orange II antibody latex particle mark, there is one to detect band and a quality control band on the nitrocellulose filter, be successively: the detection that contains the Acid Orange II protein conjugate is with 10, the quality control band 11 that contains anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell, and sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
Adopt above-mentioned Acid Orange II rapid detection card, test sample is stew in soy sauce prepared food, paprika or beverage etc., and disposal route is as follows:
Drink sample: with the beverage such as the orange juice sample pH that buy is 8.0, contains 0.05% Tween-20, and concentration is that the phosphate buffer (PBST) of 0.1mol/L is pressed the 1:1 dilution, obtains testing sample.
Stew in soy sauce prepared food, paprika sample: take by weighing the 5g sample, grind, add above-mentioned pH8.0, PBST solution 10mL, jolting 30min is used for behind the membrane filtration measuring.
When detection be with 10 and quality control band 11 manifest brownish red trace band simultaneously, testing result is negative, represents that acid orange II content does not exceed standard in the detected sample.
Be with 10 Show Colors not when detection, have only quality control band 11 to manifest the brownish red trace, testing result is positive, represents that acid orange II content exceeds standard in the detected sample.
When detection be with 10 and quality control band 11 all do not develop the color, show that then test-strips lost efficacy.
Beneficial effect of the present invention: advantage of the present invention is to detect the Acid Orange II that contains in the samples such as stew in soy sauce prepared food, paprika, beverage, is easy to preparation and saves testing cost, and is easy to use, detects fast, and highly sensitive, the result is accurate.
Description of drawings
Fig. 1 Acid Orange II rapid detection card shell and test-strips arrangement figure in the enclosure.1, shell, 2, test-strips, 3, detect fenestra, 4, well.
Show the trace synoptic diagram on the nitrocellulose filter of Fig. 2 test-strips.6, nitrocellulose filter, 10, detect band, 11, quality control band.
The detection strip structural representation of Fig. 3 Acid Orange II rapid detection card.5, supporting backboard, 6, nitrocellulose filter, 7, collaurum film or latex particle film, 8, sample pad, 9, absorbing membrane.
Embodiment
The present invention further specifies as follows in conjunction with specific embodiments referring to accompanying drawing:
A kind of Acid Orange II rapid detection card, it is that test-strips 2 is set in test card shell 1, the setting of the structure of test card shell 1 and test-strips 2 is referring to accompanying drawing 1, test card shell 1 is rectangular flat shell shape shell, long 70mm, wide 20mm, thick 5mm, shell wall thickness 1mm is made by engineering plastics.Test card shell 1 is connected with the shell block two halves by cap and constitutes, and the fenestra 3 of detection and well 4 are arranged on test card shell 1 cap.Test-strips 2 is placed in the shell block of test card shell 1.
Test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 60mm, the wide about 4mm of sheet, the about 2.5mm of thickness.Test-strips 2 is sandwich constructions: bottom is a supporting backboard 5, is the igelite thin slice, thick about 0.5mm, long 60mm, wide 4mm; The stacked stickup nitrocellulose filter 6 in supporting backboard 5 middle parts, cellulose nitrate thickness 0.5mm, long 20-25mm, wide 4mm.Two isolated horizontal demonstration trace bands are arranged on the nitrocellulose filter 6, show in the trace band referring to 2, two in accompanying drawing, one is to detect to be with 10, contains the Acid Orange II protein conjugate; Another is a quality control band 11, contains anti-rabbit antibody or anti-mouse antibody.Stacked stickup absorbing membrane 9 on supporting backboard 5 one ends, stacked stickup sample pad 8 on supporting backboard 5 the other end, absorbing membrane 9 the inners overlap with nitrocellulose filter 6 one ends respectively separately with sample pad 8 the inners, at the clinch of sample pad 8 with nitrocellulose filter 6, insert and put between the two and paste one section collaurum film or latex particle film 7, the collaurum film is the glass fibre membrane that contains resistance to acid orange II antibody colloidal gold mark, or the latex particle film is the glass fibre membrane that contains resistance to acid orange II antibody latex particle mark.Collaurum film or latex particle film 7 thick 0.5-0.8mm, long 5mm, wide 4mm.Sample pad 8 thick 0.5-0.8mm, long 20mm, wide 4mm.Absorbing membrane 9 thick 0.5-0.8mm, long 16-20mm, wide 4mm.Collaurum film or latex particle film 7, sample pad 8 and absorbing membrane 9 are 1-2mm with the nitrocellulose filter lap of splice.Absorbing membrane 9 materials are thieving paper, and sample pad 8 materials are water adsorption glass tunica fibrosas.
Acid Orange II test card of the present invention is used for detecting the detection of the Acid Orange II of samples such as stew in soy sauce prepared food, paprika, beverage.
Embodiment 2
The disposal route of sample is as follows:
Drink sample: with the beverage such as the orange juice sample pH that buy is 8.0, contains 0.05% Tween-20, and concentration is that the phosphate buffer (PBST) of 0.1mol/L is pressed the 1:1 dilution, obtains testing sample.
Stew in soy sauce prepared food, paprika sample: take by weighing the 5g sample, grind, add above-mentioned pH8.0, PBST solution 10mL, jolting 30min is used for behind the membrane filtration measuring.
When using the Acid Orange II rapid detection card to detect, earlier sample liquid to be detected is instilled into from well 4 on the sample pad 8 of Acid Orange II rapid detection card, because syphonic effect principle, drive contained resistance to acid orange II monoclonal antibody collaurum of sample liquid to be detected and collaurum film or latex particle film 7 or latex particle labels together to nitrocellulose filter 6 diffusions, observations in 5-10 minute.
The key reaction of test card is immunologic antigen and antibody response, moves on nitrocellulose filter
The antibody of collaurum or latex particle mark, on calibration tape respectively with contain the Acid Orange II protein conjugate, and the anti-rabbit antibody of quality control band or anti-mouse antibody reaction forms the brownish red band.If have Acid Orange II to be checked to exist in the sample, after sample adds, promptly react, and can not react with contained Acid Orange II protein conjugate, thereby do not develop the color with detection with antibody.The result of key reaction has following 3 kinds of situations:
1, when detection be with 10 and quality control band 11 manifest brownish red trace band simultaneously, the expression testing result negative, illustrate that acid orange II content does not exceed standard in the detected sample.
2, be with 10 Show Colors not when detection, have only quality control band 11 to manifest the brownish red trace, testing result is positive, illustrates that acid orange II content exceeds standard in the detected sample.
3, when detection be with 10 and quality control band 11 all do not develop the color, show that then detecting strip lost efficacy.
Claims (2)
1. Acid Orange II rapid detection card, it is characterized in that: in the test card shell (1) of rectangular flat shell shape test-strips (2) is set, the test card case surface has detection fenestra (3) and well (4); Test-strips (2) is upward pasted sample pad (8), collaurum film or latex particle film (7), nitrocellulose filter (6) and absorbing membrane (9) thereon successively by adhesive sticker by supporting backboard (5) and is formed; Supporting backboard middle part stacked stickup nitrocellulose filter (6), one endlap of supporting backboard is put and is pasted absorbing membrane (9), the stacked stickup sample pad of the other end (8), absorbing membrane is inner to overlap with nitrocellulose filter respectively separately with sample pad the inner, at the clinch of sample pad and nitrocellulose filter, insert and put between the two and paste one section collaurum film or latex particle film (7); The collaurum film is the glass fibre membrane that contains resistance to acid orange II antibody colloidal gold mark, or the latex particle film is the glass fibre membrane that contains resistance to acid orange II antibody latex particle mark, there is one to detect band and a quality control band on the nitrocellulose filter, be successively: the detection band (10) that contains the Acid Orange II protein conjugate, the quality control band (11) that contains anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell, and sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
2. with the detection method of claim 1 described Acid Orange II rapid detection card, it is characterized in that the content of Acid Orange II in the direct test sample of method of applied immunology, test sample is stew in soy sauce prepared food, paprika or beverage, disposal route is as follows:
Drink sample: the drink sample bought is diluted by 1:1 with pH8.0, the phosphate buffer PBST that contains the 0.1mol/L of 0.05% Tween-20, obtain testing sample;
Stew in soy sauce prepared food, paprika sample: take by weighing the 5g sample, grind, add described pH 8.0, PBST solution 10mL, jolting 30min is used for behind the membrane filtration measuring;
Manifest brownish red trace band simultaneously when detecting band (10) and quality control band (11), testing result is negative, represents that acid orange II content does not exceed standard in the detected sample;
When detecting not Show Color of band (10), have only quality control band (11) to manifest the brownish red trace, testing result is positive, represents that acid orange II content exceeds standard in the detected sample;
All do not develop the color when detecting band (10) and quality control band (11), show that then test-strips lost efficacy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011101411445A CN102162814A (en) | 2011-05-30 | 2011-05-30 | Quick detection card for acid orange II and detection method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011101411445A CN102162814A (en) | 2011-05-30 | 2011-05-30 | Quick detection card for acid orange II and detection method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102798713A (en) * | 2012-08-08 | 2012-11-28 | 广州市质量监督检测研究院 | Detection kit for food red 10 and preparation method thereof |
| CN105301244A (en) * | 2014-06-03 | 2016-02-03 | 北京勤邦生物技术有限公司 | Enzyme linked immunosorbent assay kit for detecting acid orange and application of enzyme linked immunosorbent assay kit |
| CN106370847A (en) * | 2016-08-22 | 2017-02-01 | 广州达元食品安全技术有限公司 | Rapid detection kit of lemon yellow in food |
| CN106568965A (en) * | 2016-10-14 | 2017-04-19 | 广州安诺食品科学技术有限公司 | Auramine O colloidal gold test card and preparation method thereof |
| CN106645683A (en) * | 2016-10-14 | 2017-05-10 | 广州安诺食品科学技术有限公司 | Orange II colloidal gold testing card and preparation method thereof |
| CN112578113A (en) * | 2020-09-22 | 2021-03-30 | 苏州市药品检验检测研究中心 | Abietic acid colloidal gold detection card and manufacturing method thereof |
| CN112578112A (en) * | 2020-09-22 | 2021-03-30 | 苏州市药品检验检测研究中心 | 808scarlet colloidal gold detection card and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003006676A2 (en) * | 2001-07-13 | 2003-01-23 | Nanosphere, Inc. | Method for immobilizing molecules onto surfaces |
| CN101625368A (en) * | 2009-08-04 | 2010-01-13 | 无锡安迪生物工程有限公司 | Casein rapid detection card and detection method thereof |
| CN101915831A (en) * | 2010-08-25 | 2010-12-15 | 南开大学 | Enzyme-linked immunoassay kit for acid orange II |
| CN101957374A (en) * | 2010-09-17 | 2011-01-26 | 无锡安迪生物工程有限公司 | Benzopyrene rapid detection card |
-
2011
- 2011-05-30 CN CN2011101411445A patent/CN102162814A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003006676A2 (en) * | 2001-07-13 | 2003-01-23 | Nanosphere, Inc. | Method for immobilizing molecules onto surfaces |
| CN101625368A (en) * | 2009-08-04 | 2010-01-13 | 无锡安迪生物工程有限公司 | Casein rapid detection card and detection method thereof |
| CN101915831A (en) * | 2010-08-25 | 2010-12-15 | 南开大学 | Enzyme-linked immunoassay kit for acid orange II |
| CN101957374A (en) * | 2010-09-17 | 2011-01-26 | 无锡安迪生物工程有限公司 | Benzopyrene rapid detection card |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102798713A (en) * | 2012-08-08 | 2012-11-28 | 广州市质量监督检测研究院 | Detection kit for food red 10 and preparation method thereof |
| CN102798713B (en) * | 2012-08-08 | 2014-08-27 | 广州市质量监督检测研究院 | Detection kit for food red 10 and preparation method thereof |
| CN105301244A (en) * | 2014-06-03 | 2016-02-03 | 北京勤邦生物技术有限公司 | Enzyme linked immunosorbent assay kit for detecting acid orange and application of enzyme linked immunosorbent assay kit |
| CN106370847A (en) * | 2016-08-22 | 2017-02-01 | 广州达元食品安全技术有限公司 | Rapid detection kit of lemon yellow in food |
| CN106568965A (en) * | 2016-10-14 | 2017-04-19 | 广州安诺食品科学技术有限公司 | Auramine O colloidal gold test card and preparation method thereof |
| CN106645683A (en) * | 2016-10-14 | 2017-05-10 | 广州安诺食品科学技术有限公司 | Orange II colloidal gold testing card and preparation method thereof |
| CN112578113A (en) * | 2020-09-22 | 2021-03-30 | 苏州市药品检验检测研究中心 | Abietic acid colloidal gold detection card and manufacturing method thereof |
| CN112578112A (en) * | 2020-09-22 | 2021-03-30 | 苏州市药品检验检测研究中心 | 808scarlet colloidal gold detection card and preparation method thereof |
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Application publication date: 20110824 |