CN204058439U - A kind of instrument cases for nucleic acid rapid extraction - Google Patents
A kind of instrument cases for nucleic acid rapid extraction Download PDFInfo
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- CN204058439U CN204058439U CN201420387897.3U CN201420387897U CN204058439U CN 204058439 U CN204058439 U CN 204058439U CN 201420387897 U CN201420387897 U CN 201420387897U CN 204058439 U CN204058439 U CN 204058439U
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Abstract
The utility model is used for the instrument cases of nucleic acid rapid extraction, comprise a pipe support, the some test tubes be placed on pipe support, nucleic acid-extracting apparatus and an outer packaging, described pipe support establishes frame leg and upper and lower two-layer dividing plate, on upper and lower dividing plate, correspondence is distributed with holding hole, and the upper spacer of pipe support is divided into for the firstth district of holding test tubes and the secondth district for placing nucleic acid-extracting apparatus.The utility model is mainly used in rapid extraction nucleic acid (comprising RNA and DNA) from various clinical sample, after the steps such as clinical sample cracking process, syringe pump, nucleic acid absorption film adsorbs nucleic acid, rinsing liquid rinsing nucleic acid absorption film, elution nucleic acid, pure nucleic acid solution can be obtained and test for downstream.
Description
Technical field
The utility model relate to a kind of can from various clinical sample the instrument cases of rapid extraction nucleic acid.
Background technology
Nucleic acid extraction has become most important, the most basic operation in molecular biology experiment technology, but at present the extraction scheme of nucleic acid is many, and complicated and simplely differs.The successful extraction completing all dependence nucleic acid of the related experiment such as molecular diagnosis, nucleic acid sequencing, functional genome, traditional operating process can realize whole automatizations, but the instrument of these costlinesses can not be promoted the use of on a large scale, China is still developing country at present, vast different medical unit can not be equipped with complicated instrument, therefore, provide a kind of nucleic acid-extracting apparatus of good economy performance necessary.
CN101684463A proposes a kind of trace clinical sample nucleic acid rapid extraction device, by sharp-crested suction pipe, strainer with film and suction unit (syringe or plastic blister) three part composition, it has aspirated nucleic acid absorption by three times, cleaning and elution process, fast and conveniently can extract nucleic acid from sample, right blemish in an otherwise perfect thing be, sharp-crested suction pipe, strainer with film and suction unit three incorporating aspects mode single and for fixed installation, (Fig. 2 of CN101684463A when making suction unit by plastic blister, Fig. 3) third time suction carries out that Nucleic Acid Elution is actual can not complete that (waste liquid of front twice suction cannot be discharged, elutriant with nucleic acid is mixed in waste liquid), and when using syringe to make suction unit, elutriant with nucleic acid enters syringe not only can be infected with syringe formation biologic garbage, trace sample amplifying nucleic acid remarks emitter also can be caused to be infected with and lose cause detect inaccurate.
On the other hand, owing to often having time limit requirement in clinical detection and diagnosis, need rapid extraction and detect nucleic acid, reagent and the detection utensil of Routine Test Lab are classified numerous and jumbled and can not detect pointedly, are difficult to realize efficient detection; And grass-roots unit often condition is limited, be difficult to again the short period of time to gather together enough detection reagent and utensil, therefore Rapid nucleic acid extractive technique be not easy to penetration and promotion.Be necessary the nucleic acid extraction instrument cases product researched and developed a kind of quick, easy, easy handling, can promote the use of.
Utility model content
The utility model object is to provide a kind of combination to have nucleic acid rapid extraction device and relevant appliance, every person-portion independent packaging, the instrument cases for nucleic acid rapid extraction easy to use.
The utility model is used for the instrument cases of nucleic acid rapid extraction, comprise a pipe support, the some test tubes be placed on pipe support, nucleic acid-extracting apparatus and an outer packaging, described pipe support establishes frame leg and upper and lower two-layer dividing plate, on upper and lower dividing plate, correspondence is distributed with holding hole, and the upper spacer of pipe support is divided into for the firstth district of holding test tubes and the secondth district for placing nucleic acid-extracting apparatus.
Firstth district is provided with multiple subregion, and each subregion establishes a holding hole for placing the test tube of reference numeral; The holding hole in corresponding pipe support first district, is equipped with the test tube of same quantity, and the label that on test tube, all mark is consistent with dividing plate subregion, the test tube of identical label is fixedly mounted in the holding hole of pipe support.
The nucleic acid-extracting apparatus being positioned over the secondth district comprises two syringes and each one of adsorption film pipe, interconnecting device and sample needle, correspondingly with syringe profile in secondth district of pipe support upper spacer be provided with two accommodating holes, correspondingly with other three portion profile above-mentioned be provided with other three accommodating holes, the parts of coupling are placed in relative accommodating hole.
The interconnecting device of described nucleic acid-extracting apparatus is hollow round table shape, and interconnecting device is connected in the middle of syringe and adsorption film pipe and syringe, interconnecting device are communicated with adsorption film pipe fluid path; Bottom in adsorption film pipe is provided with adsorption film.
Adsorption film pipe is the body of both ends open, and one end is the opening end of this body, and the other end fixes an osculum pipe connecting, and adsorption film is arranged on this osculum pipe connecting one end; Adsorption film pipe opening end size is mated with the outside dimension of interconnecting device.
Syringe comprises cylindrical shell, being placed in the tubulose Connection Block of barrel front end and being placed in cylindrical shell can the push rod of push-and-pull, the external diameter of described osculum pipe connecting is equal with the Connection Block outside dimension of syringe, described interconnecting device establishes intermediate throughholes, and intermediate throughholes size is mated with the Connection Block outside dimension of syringe; Described sample needle comprises needle stand and syringe needle, and the external diameter of described osculum pipe connecting mates with the needle stand internal diameter of described sample needle.
Interconnecting device one end is communicated with syringe, and the other end is communicated with the osculum pipe connecting of adsorption film pipe, forms the drawing liquid mode of this device.
Or interconnecting device one end is communicated with syringe, and the other end is socketed in the opening end of adsorption film pipe, the osculum pipe connecting of adsorption film pipe is communicated with sample needle, and what form this device pushes away liquid mode; Profile is that the little head end outside dimension of round table-like interconnecting device mates with the opening end of adsorption film pipe, and this opening end is from the little head end socket of round table-like interconnecting device.
Concrete, the utility model is the instrument cases for DNA extraction, and the firstth district of pipe support is square, is provided with five subregion A-E, five subregions other four subregion symmetricaerofoil dispersion centered by one of them are arranged, each subregion establishes a holding hole for placing the test tube of reference numeral; Adsorption film in adsorption film pipe is the pellosil of energy adsorption of DNA.
Concrete, the utility model is for the instrument cases that RNA extracts or RNA and DNA extracts jointly, firstth district of pipe support is rectangle, it is distributed with nine subregion A-I, other eight symmetrical settings of subregion centered by one of them subregion, each subregion establishes a holding hole, and wherein each subregion of A, B respectively establishes a main aperture (A and B) and a secondary orifices (A+ and B+), and the test tube of reference numeral is placed in each holding hole; Adsorption film in adsorption film pipe can adsorb the pellosil of RNA or can adsorb the pellosil of RNA and DNA.
Adopt above scheme, feature of the present utility model has: multiduty interconnecting device can connect different parts simultaneously, utilizes syringe to instead of high speed centrifuge simultaneously, utilizes extraction element just can reach the effect of separation in liquid phase heterogeneous system.The utility model, by component combination packaging various needed for nucleic acid extraction, conveniently can be taken, be more convenient for carrying, be suitable for the execute-in-place to biological specimen.
The utility model can as the simple and direct means of one of before nucleic acid amplification, biological specimen being carried out to nucleic acid extraction, the sample purity extracted enough meets the requirement of next step nucleic acid amplification, simultaneously without any need for instrument, operational safety and simple, single use, thus pathogenic agent or host nucleic acids extraction can be widely used in, for clinical detection and diagnosis.The utility model extraction element is with low cost, again without any need for specific apparatus while simple to operate, operating time is short, approximately only needs 10 ?15 minutes, and thus the utility model can be widely used in the relatively poor basic hospital of some experiment conditions and economically less developed region or country; Meanwhile, the demand, particularly emergency room of high-level hospital and the nucleic acid quick diagnosis of bedside can also be met well.
Accompanying drawing explanation
Fig. 1 is the subregion of the utility model embodiment 1 nucleic acid rapid extraction instrument cases pipe support and forms schematic diagram;
Fig. 2 is the subregion of the utility model embodiment 2 nucleic acid rapid extraction instrument cases pipe support and forms schematic diagram;
Fig. 3 A ?Fig. 3 D be formation and the structure iron of the utility model amplifying nucleic acid extraction element;
Fig. 4 A ?Fig. 4 B be the utility model amplifying nucleic acid extraction element exercise pumping function time decomposition and constitutional diagram;
Fig. 5 A ?Fig. 5 B be the utility model amplifying nucleic acid extraction element exercise pushing function time decomposition and constitutional diagram.
Embodiment
The utility model is used for the instrument cases of nucleic acid rapid extraction, is mainly used in rapid extraction nucleic acid (comprising RNA and DNA) from various clinical sample.Mainly comprise syringe, the interconnecting device of hollow, nucleic acid absorption film pipe, sample needle, hold the part such as various pipes, pipe support of different reagent, after the steps such as clinical sample cracking process, syringe pump, nucleic acid absorption film adsorbs nucleic acid, rinsing liquid rinsing nucleic acid absorption film, elution nucleic acid, pure nucleic acid solution can be obtained and test for downstream.
See figure 1 ?shown in Fig. 2, first the utility model instrument cases are equipped with a pipe support 9, similar with traditional test-tube stand, and pipe support 9 establishes frame leg and upper and lower two-layer dividing plate, on upper and lower dividing plate, correspondence is distributed with holding hole, and general lower partition hole is slightly less than upper partition hole; In the utility model, the upper spacer of pipe support 9 is divided into two functional areas, the firstth district and the secondth district, wherein:
Firstth district is provided with multiple subregion, and each subregion establishes a holding hole for placing the test tube of reference numeral; The holding hole in corresponding pipe support 9 first district, is equipped with the test tube of same quantity, the label that on test tube, all mark is consistent with dividing plate subregion, and is fixedly mounted in the holding hole of pipe support 9 by the test tube of identical label.
Secondth district is mainly used in placing nucleic acid-extracting apparatus, nucleic acid-extracting apparatus mainly comprise syringe 1, adsorption film pipe 2, interconnecting device 3 and sample needle 4 four parts (see Fig. 3 A ?Fig. 3 D), in the secondth district of pipe support 9 upper spacer, correspondingly with syringe 1 profile be provided with two accommodating holes 91, corresponding with other three portion profile above-mentioned be provided with accommodating hole 92 ?94, in addition the parts of coupling label are put into relative accommodating hole with fixing, wherein comprise two syringes 1 and each one of adsorption film pipe 2, interconnecting device 3 and sample needle 4.
So, each test tube is fixedly mounted on by label in the holding hole in pipe support 9 first district, each parts of nucleic acid-extracting apparatus are fixedly mounted in the holding hole in pipe support 9 second district by coupling label, be equipped with an outer packaging (bag or box) again, namely obtain the instrument cases of the utility model for nucleic acid rapid extraction.
The utility model is further described below with specific embodiment.
Embodiment 1:DNA extracting tool box
In the present embodiment, in instrument cases product, the structure of pipe support 9 as hereinbefore, feature is: the firstth district is square, as shown in Figure 1 it is distributed with five subregion A-E, for making operational stability good, five subregions other four subregion symmetricaerofoil dispersion centered by one of them (E district) are arranged, each subregion establishes a holding hole for placing the test tube of reference numeral.
Secondth district equally for placing syringe 1 in nucleic acid-extracting apparatus, adsorption film pipe 2, interconnecting device 3 and sample needle 4 four parts, wherein:
Syringe 1 is common tubular disposable syringe or the allied equipment exercising syringe function, and it has cylindrical shell 11, be placed in cylindrical shell 11 front end for connecting the tubulose Connection Block 12 of syringe needle and be placed in cylindrical shell 11 can the push rod 13 of push-and-pull; Sample needle 4 is common injection needles, comprises needle stand 41 and syringe needle 42.
Interconnecting device 3 is cylinder or the round platform of hollow, and its intermediate throughholes 31 size is mated with syringe 1 tubulose Connection Block 12 outside dimension, makes this tubulose Connection Block 12 can be inserted in interconnecting device 3 intermediate throughholes 31; Separately, interconnecting device 3 appearance is cylindric or round table-like, and its outside dimension mates with adsorption film pipe 2, below describes in detail; Interconnecting device 3 can have elastic material such as rubber, plastics and make.
Adsorption film pipe 2 is the body of both ends open, available plastic material is made, its one end is the opening end 21 of this body, the other end fixes an osculum pipe connecting 22, adsorption film pipe 2 inner bottom part (pipe connecting 22 one end is called the end) establishes adsorption film 23 (the pellosil Silica membrane of adsorption of DNA is equipped with in commercial goods in this example); The both ends open of adsorption film pipe 2 all can be connected with interconnecting device 3, and wherein opening end 21 size is mated with the outside dimension of cylindric interconnecting device 3, or mates interconnecting device 3 can be socketed with opening end 21 with the little head end outside dimension of round table-like interconnecting device; Osculum pipe connecting 22 external diameter of adsorption film pipe 2 is identical with syringe 1 Connection Block 12, both mated with needle stand 41 internal diameter of sample needle 4, again with interconnecting device 3 intermediate throughholes 31 size match, this osculum pipe connecting 22 can be inserted in needle stand 41 and connect sample needle 4, connection adapter 3 in intermediate throughholes 31 can be inserted into again.
So, each test tube A-E is fixedly mounted on by label in the holding hole A-E in pipe support 9 first district, each parts of nucleic acid-extracting apparatus are fixedly mounted on by label in the holding hole in pipe support 9 second district, be equipped with an outer packaging (bag or box) again, namely obtain the instrument cases of the utility model for DNA rapid extraction.
Further, each test tube in above-mentioned instrument cases can be made with polypropylene material, and is furnished with lid, loads onto the reagent (a sample consumption) for DNA extraction wherein, then form DNA extraction kit product.Concrete, the reagent that can load in this routine each test tube is: A manages: lysate; Be mainly used in the cell in lysed sample.B manages: washing lotion 1; Mostly be 75% alcohol, the impurity on clean adsorption film.C manages: washing lotion 2; Mostly be 95% alcohol, the impurity on clean adsorption film.D manages: elutriant; Mostly be Tris-HCL or the distilled water of 10mM, for by nucleic acid from wash-out adsorption film.E manages: blank pipe, as the solution containing sample nucleic acid that collection tube obtains for holding wash-out.The holding hole of pipe support and each pipe one_to_one corresponding on color mod sum label, convenient placement and operation.After being attached with reagent, DNA rapid extraction instrument cases become DNA rapid extraction test kit, more convenient to the DNA extraction of sample.
Above instrument cases or test kit DNA rapid extraction from sample is utilized to comprise the following steps:
A) reagent indicates packing: be filled in instrument cases in each test tube by each person-portions (a sample consumption) such as involved lysate, 75% alcohol, 95% alcohol, elutriant point, and is denoted as the different letter of A-D and color to each pipe; Each test tube is placed in the holding hole A-D in pipe support 9 first district, is placed on by blank pipe E pipe in the holding hole E in pipe support 9 first district;
B) clinical sample is added be equipped with in the A pipe of lysate, and mix;
C) packageable nucleic acid extraction element a: syringe 1, adsorption film pipe 2 and interconnecting device 3 are taken out from pipe support 9 second district, the Connection Block 12 of syringe 1 is connected with interconnecting device 3 one end, the other end connects the osculum pipe connecting 22 of adsorption film pipe 2, make adsorption film 23 rear (in the utility model with syringe user to definition before and after, immediate shipment syringe needle one end is defined as " front ", when push rod extracts, direction of motion is defined as " afterwards "), namely combine the drawing liquid form (as can be seen from figures 4a-b) of nucleic acid rapid extraction device.
D) one inhales: the drawing liquid form using the nucleic acid-extracting apparatus of above-mentioned assembling, aspirate from A pipe sample cracking mixed solution with the opening end of adsorption film pipe, utilize the suction that syringe pump produces, nucleic acid component in sample is adsorbed on adsorption film because of the specific adsorption of film when flowing through adsorption film, filtrate being has the waste liquid of the impurity such as cell debris and protein, waste liquid enter syringe cavity and waste liquid merely through this adsorption film once;
E) two inhale: continue to use drawing liquid form, from B pipe, aspirate washing lotion 1 with the opening end of adsorption film pipe, utilize the suction that syringe pump produces, clean adsorption film by washing lotion 1, clean the protein or other composition that remain on adsorption film, filtrate enters syringe cavity; Again from C pipe, aspirate washing lotion 2 with the opening end of adsorption film pipe, utilize the suction that syringe pump produces, clean adsorption film by washing lotion 1, clean the protein or other composition that remain on adsorption film, filtrate enters syringe cavity; Dismounting adsorption film pipe 2 and interconnecting device 3, place with the syringe 1 crossed;
F) reverse packageable nucleic acid extraction element: the syringe 1 and the sample needle 4 that take out another cleaning, the elutriant in D pipe is first sucked with this syringe, the Connection Block 12 of syringe 1 is connected with interconnecting device 3 one end, the other end stretches into the opening end 21 of adsorption film pipe 2, and the osculum pipe connecting 22 of adsorption film pipe 2 connects the needle stand 41 of sample needle 4 again, combine the liquid form that pushes away (as shown in figs. 5 a-b) of nucleic acid rapid extraction device.
G) one push away: what use nucleic acid-extracting apparatus pushes away liquid form, sample needle 4 is facing to E pipe (E pipe is as collection tube) herein, elutriant in syringe cavity is joined in adsorption film pipe, utilize the pressure of syringe pushing generation by eluent stream through adsorption film, and the nucleic acid component wash-out be adsorbed on film is pushed collect to E pipe, complete the leaching process of DNA amplifying nucleic acid in sample.
What E pipe like this was collected is the DNA solution not containing other impurity, can be used for follow-up nucleic acid amplification and detection.
Embodiment 2:RNA extracting tool box
In the present embodiment, in instrument cases product, the structure of pipe support 9 as hereinbefore, feature is: the firstth district of pipe support 9 is rectangle (as Fig. 2), it is distributed with nine subregion A-I, same other eight symmetrical settings of subregion centered by one of them subregion (I district), each subregion establishes a holding hole, and wherein each subregion of A, B respectively establishes a main aperture (A and B) and a secondary orifices (A+ and B+).
Second district place nucleic acid-extracting apparatus substantially the same manner as Example 1, slightly differentiated be in adsorption film pipe 2 installing adsorption film 23 be the pellosil Silica membrane that can adsorb RNA.Same section repeats no more.
So, each test tube A-I is fixedly mounted on by label in the holding hole A-I in pipe support 9 first district, each parts of nucleic acid-extracting apparatus are fixedly mounted on by label in the holding hole in pipe support 9 second district, be equipped with an outer packaging (bag or box) again, namely obtain the instrument cases of the utility model for RNA rapid extraction.
Further, further, each test tube in above-mentioned instrument cases can be made with polypropylene material, and is furnished with lid, loads onto the reagent (a sample consumption) extracted for RNA wherein, then form RNA and extract reagent kit product.In this example, the reagent that can load in each test tube is: A manages: RNA enzyme inhibitors (powder); Be mainly used in suppressing the RNA enzyme in sample, at utmost preserve the extracted amount of RNA.A+ manages: AVE solution; Be mainly used in dissolving RNA enzyme inhibitors (powder).B manages: RNA bonding agent (powder); When being mainly used to extract nucleic acid for trace sample, help the effect of high efficiente callback nucleic acid.B+ manages: AVE solution; Be mainly used in dissolving RNA bonding agent (powder).C manages: lysate; Be mainly used in the cell in lysed sample.D manages: 99% alcohol; Be mainly used in precipitate nucleic acids, nucleic acid separated out and is better adsorbed on adsorption film.E manages: washing lotion 1; Mostly be 70% alcohol, the impurity on clean adsorption film.F manages: washing lotion 2; Mostly be 95% alcohol, the impurity on clean adsorption film.G manages: washing lotion 3; Mostly be 99% alcohol, the impurity on clean adsorption film also contributes to dry adsorption film.H manages: elutriant; Mostly be Tris-HCL or the distilled water of 10mM, for by nucleic acid from wash-out adsorption film.I manages: blank pipe, as the solution containing nucleic acid that collection tube obtains for holding wash-out.Holding hole on pipe support and each test tube are identified at one_to_one corresponding on color mod sum label, convenient placement and operation.
After being attached with reagent, RNA rapid extraction instrument cases become RNA rapid extraction test kit, and it is more convenient to extract the RNA of sample.
Utilize above instrument cases or test kit rapid extraction RNA from sample.Comprise the following steps:
A) reagent indicates packing: be filled in instrument cases in each test tube by each person-portion (a sample consumption) point by involved above-mentioned each reagent, and is denoted as the different letter of A-H and color to each pipe; Each test tube is placed in the holding hole A-H in pipe support 9 first district, is placed on by blank pipe I pipe in the holding hole I in pipe support 9 first district;
B) the AVE solution in A+ pipe is joined in the middle of A pipe powder dissolve RNA enzyme inhibitors; AVE solution in B+ pipe is joined in the middle of B pipe powder and dissolve RNA bonding agent (powder); Clinical sample is added and is equipped with in the C pipe of lysate, then add the RNA enzyme inhibitors of dissolving and the RNA bonding agent of dissolving, and mix; Finally the solution in D pipe is joined in C pipe, again mix, so far complete cracking mixed solution, next step operation can be carried out.
C) packageable nucleic acid extraction element a: syringe 1, adsorption film pipe 2 and interconnecting device 3 are taken out from pipe support 9 second district, the Connection Block 12 of syringe 1 is connected with interconnecting device 3 one end, the other end connects the osculum pipe connecting 22 of adsorption film pipe 2, make adsorption film 23 rear (in the utility model with syringe user to definition before and after, immediate shipment syringe needle one end is defined as " front ", when push rod extracts, direction of motion is defined as " afterwards "), namely combine the drawing liquid form (as can be seen from figures 4a-b) of nucleic acid rapid extraction device.
D) one inhales: the drawing liquid form using the nucleic acid-extracting apparatus of above-mentioned assembling, aspirate from C pipe sample cracking mixed solution with the opening end of adsorption film pipe, utilize the suction that syringe pump produces, nucleic acid component in sample is adsorbed on adsorption film because of the specific adsorption of film when flowing through adsorption film, filtrate being has the waste liquid of the impurity such as cell debris and protein, waste liquid enter syringe cavity and waste liquid merely through this adsorption film once;
E) two inhale: continue to use drawing liquid form, from E pipe, aspirate washing lotion 1 with the opening end of adsorption film pipe, utilize the suction that syringe pump produces, clean adsorption film by washing lotion 1, clean the protein or other composition that remain on adsorption film, filtrate enters syringe cavity; Again from F pipe, aspirate washing lotion 2 with the opening end of adsorption film pipe, utilize the suction that syringe pump produces, clean adsorption film by washing lotion 2, clean the protein or other composition that remain on adsorption film, filtrate enters syringe cavity; Again from G pipe, aspirate washing lotion 3 with the opening end of adsorption film pipe, utilize the suction that syringe pump produces, clean adsorption film by washing lotion 3, clean the protein or other composition that remain on adsorption film, filtrate enters syringe cavity; Dismounting adsorption film pipe 2 and interconnecting device 3, place with the syringe 1 crossed;
F) reverse packageable nucleic acid extraction element: the syringe 1 and the sample needle 4 that take out another cleaning, the elutriant in H pipe is first sucked with this syringe, the Connection Block 12 of syringe 1 is connected with interconnecting device 3 one end, the other end stretches into the opening end 21 of adsorption film pipe 2, and the osculum pipe connecting 22 of adsorption film pipe 2 connects the needle stand 41 of sample needle 4 again, combine the liquid form that pushes away (as shown in figs. 5 a-b) of nucleic acid rapid extraction device.
G) one push away: what use nucleic acid-extracting apparatus pushes away liquid form, sample needle 4 is facing to I pipe, elutriant in syringe cavity is joined in adsorption film pipe, utilize the pressure of syringe pushing generation by eluent stream through adsorption film, and the nucleic acid component wash-out be adsorbed on film is pushed collect to I pipe, complete the leaching process of RNA in sample.
The solution being the RNA not containing other impurity of I pipe collection like this, can be used for follow-up nucleic acid amplification and detection.Be applied to the downstream experiment that microorganism detection, detection in Gene Mutation, single nucleotide polymorphism detection etc. relate to nucleic acid.
Illustrate: test tube in the utility model instrument cases is because conventional test tube wall is all polyacrylic material, tube wall there is electrostatic, meeting adsorbs nucleic acid, therefore in extraction purification process, adopting RNA bonding agent, (such as embodiment 2 is contained in the reagent in B pipe, AVE solution in B+ pipe is for dissolving this RNA bonding agent) nucleic acid can be helped to remove electrostatic effect, usually add in stepb, nucleic acid RNA is made to be adsorbed on purification column very well, can elution efficiency be improved, thus reach recovery sample amplifying nucleic acid large as far as possible.If test tube adopts other material of adsorbs nucleic acid to make, the RNA bonding agent in B pipe and the AVE solution in B+ pipe optional.
The common extracting tool box of embodiment 3:RNA and DNA
The present embodiment is used for from sample, implement RNA and DNA and jointly extracts and detect, this instrument cases product is substantially the same manner as Example 2, slightly differentiated be in adsorption film pipe 2 installing adsorption film 23 be the pellosil Silica membrane that simultaneously can adsorb RNA and DNA.
In the present embodiment, agents useful for same is identical with embodiment 2, RNA and DNA rapid extraction instrument cases become RNA and DNA rapid extraction test kit, and it is more convenient to extract while RNA and DNA to sample.
The process utilizing above instrument cases and test kit to extract RNA with DNA fast altogether from sample operates also identical with embodiment 2, finally completes the common extraction of RNA and DNA in sample.The present embodiment and embodiment 2 same section repeat no more.
May be there is the situation of RNA and DNA in the present embodiment application, collect the solution for RNA and DNA not containing other impurity obtained in the sample to which, can be used for follow-up nucleic acid amplification and detection, somatotype.Be applied to the downstream experiment that microorganism detection, detection in Gene Mutation, single nucleotide polymorphism detection etc. relate to nucleic acid.
The utility model incorporates the whole instruments extracting nucleic acid from sample, can as the simple and direct means of one of before nucleic acid amplification, biological specimen being carried out to nucleic acid extraction after adding reagent, the nucleic acid purity extracted enough meets the requirement of next step amplification, simultaneously without any need for instrument, operational safety and simple, single use, thus pathogenic agent or host nucleic acids extraction can be widely used in, for clinical detection and diagnosis.The utility model extraction element is with low cost, again without any need for specific apparatus while simple to operate, operating time is short, approximately only needs 10 ?15 minutes, and thus the utility model can be widely used in the relatively poor basic hospital of some experiment conditions and economically less developed region or country; Meanwhile, the demand, particularly emergency room of high-level hospital and the nucleic acid quick diagnosis of bedside can also be met well.
Claims (10)
1. for the instrument cases of nucleic acid rapid extraction, it is characterized in that, comprise a pipe support, the some test tubes be placed on pipe support, nucleic acid-extracting apparatus and an outer packaging, described pipe support establishes frame leg and upper and lower two-layer dividing plate, on upper and lower dividing plate, correspondence is distributed with holding hole, and the upper spacer of pipe support is divided into for the firstth district of holding test tubes and the secondth district for placing nucleic acid-extracting apparatus.
2. instrument cases according to claim 1, is characterized in that, the firstth district is provided with multiple subregion, and each subregion establishes a holding hole for placing the test tube of reference numeral; The holding hole in corresponding pipe support first district, is equipped with the test tube of same quantity, and the label that on test tube, all mark is consistent with dividing plate subregion, the test tube of identical label is fixedly mounted in the holding hole of pipe support.
3. instrument cases according to claim 1, it is characterized in that, the nucleic acid-extracting apparatus being positioned over the secondth district comprises two syringes and each one of adsorption film pipe, interconnecting device and sample needle, correspondingly with syringe profile in secondth district of pipe support upper spacer be provided with two accommodating holes, correspondingly with other three portion profile above-mentioned be provided with other three accommodating holes, the parts of coupling are placed in relative accommodating hole.
4. instrument cases according to claim 3, is characterized in that, the interconnecting device of described nucleic acid-extracting apparatus is hollow round table shape, and interconnecting device is connected in the middle of syringe and adsorption film pipe and syringe, interconnecting device are communicated with adsorption film pipe fluid path; Bottom in adsorption film pipe is provided with adsorption film.
5. instrument cases according to claim 4, is characterized in that, adsorption film pipe is the body of both ends open, and one end is the opening end of this body, and the other end fixes an osculum pipe connecting, and adsorption film is arranged on this osculum pipe connecting one end; Adsorption film pipe opening end size is mated with the outside dimension of interconnecting device.
6. instrument cases according to claim 5, it is characterized in that, syringe comprises cylindrical shell, being placed in the tubulose Connection Block of barrel front end and being placed in cylindrical shell can the push rod of push-and-pull, the external diameter of described osculum pipe connecting is equal with the Connection Block outside dimension of syringe, described interconnecting device establishes intermediate throughholes, and intermediate throughholes size is mated with the Connection Block outside dimension of syringe; Described sample needle comprises needle stand and syringe needle, and the external diameter of described osculum pipe connecting mates with the needle stand internal diameter of described sample needle.
7. instrument cases according to claim 6, is characterized in that, interconnecting device one end is communicated with syringe, and the other end is communicated with the osculum pipe connecting of adsorption film pipe, forms the drawing liquid mode of this device.
8. instrument cases according to claim 6, is characterized in that, interconnecting device one end is communicated with syringe, and the other end is socketed in the opening end of adsorption film pipe, and the osculum pipe connecting of adsorption film pipe is communicated with sample needle, and what form this device pushes away liquid mode; Profile is that the little head end outside dimension of round table-like interconnecting device mates with the opening end of adsorption film pipe, and this opening end is from the little head end socket of round table-like interconnecting device.
9. according to the arbitrary described instrument cases of claim 1 to 8, it is characterized in that, for the instrument cases for DNA extraction, firstth district of pipe support is square, be provided with five subregion A-E, five subregions other four subregion symmetricaerofoil dispersion centered by one of them are arranged, each subregion establishes a holding hole for placing the test tube of reference numeral; Adsorption film in adsorption film pipe is the pellosil of energy adsorption of DNA.
10. according to the arbitrary described instrument cases of claim 1 to 8, it is characterized in that, for the instrument cases jointly extracted for RNA extraction or RNA and DNA, firstth district of pipe support is rectangle, it is distributed with nine subregion A-I, other eight symmetrical settings of subregion centered by one of them subregion, each subregion establishes a holding hole, wherein each subregion of A, B respectively establishes a main aperture and a secondary orifices, and the test tube of reference numeral is placed in each holding hole; Adsorption film in adsorption film pipe can adsorb the pellosil of RNA or can adsorb the pellosil of RNA and DNA.
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| CN201420387897.3U CN204058439U (en) | 2013-07-12 | 2014-07-14 | A kind of instrument cases for nucleic acid rapid extraction |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073429A (en) * | 2013-07-12 | 2014-10-01 | 中国人民解放军疾病预防控制所 | Tool box and kit for rapid extraction of nucleic acid, and method for rapid extraction of nucleic acid |
| WO2019001228A1 (en) * | 2017-06-30 | 2019-01-03 | 开启基因股份有限公司 | Fixed tube of nucleic acid extraction component, and nucleic acid extraction component |
-
2014
- 2014-07-14 CN CN201420387897.3U patent/CN204058439U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073429A (en) * | 2013-07-12 | 2014-10-01 | 中国人民解放军疾病预防控制所 | Tool box and kit for rapid extraction of nucleic acid, and method for rapid extraction of nucleic acid |
| WO2019001228A1 (en) * | 2017-06-30 | 2019-01-03 | 开启基因股份有限公司 | Fixed tube of nucleic acid extraction component, and nucleic acid extraction component |
| US11365406B2 (en) | 2017-06-30 | 2022-06-21 | Catchgene Co., Ltd. | Fixed tube of nucleic acid extraction component, and nucleic acid extraction component |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104073429A (en) | 2014-10-01 |
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