CN103293049A - Serum separation method and serum separation device - Google Patents
Serum separation method and serum separation device Download PDFInfo
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- CN103293049A CN103293049A CN2013102036696A CN201310203669A CN103293049A CN 103293049 A CN103293049 A CN 103293049A CN 2013102036696 A CN2013102036696 A CN 2013102036696A CN 201310203669 A CN201310203669 A CN 201310203669A CN 103293049 A CN103293049 A CN 103293049A
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- serum separation
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Abstract
The invention aims to provide a novel serum separation method and a novel serum separation device. The device consists of a blood inlet, a serum separation column, a serum outlet and a serum collection tube. The device adopts the principle that the whole blood is added from a sample inlet, a blood sample flows through the serum separation column under the action of gravity or a certain negative pressure, blood cells are retained, and serum flows out of a pore between micro-beads and enters the serum collection tube. The serum separation method and the serum separation device, provided by the invention, are used for extracting the serum, and have the advantages of simple equipment, easiness in operation and high separation efficiency.
Description
Technical field
The present invention relates to a kind of serum separation method and device.
Background technology
Based on for example biochemical medicine detection of blood, urine and saliva of human body fluid, can provide the important information of reaction health of people state.At present, blood testing is pattern detection the most widely in biomedicine, clinical diagnosis, the health quarantine diagnosis, but there is very big interference in erythrocytic existence to the spectral analysis in the blood testing, therefore how to isolate focus and difficult point problem that serum is blood testing research always quickly and easily from whole blood.
At present, most hospitals and laboratory also are to use large-scale hydro-extractor separation of serum and haemocyte, the method need extract the blood sample of ml vol in patient's body, and really for detection of sample tens of and even number microlitres only, cause a large amount of wastes of sample; And this type of centrifugation apparatus volume is big, complicated in mechanical structure, serum detachment process and detection are separately independently to carry out, easily cause loss and the pollution of blood sample, be not suitable for the integrated of clinical diagnosis and miniature analytic system, can not satisfy the needs of micro-full analytical system development in the medical diagnosis, therefore be necessary to develop a kind of new differential from technology.
Summary of the invention
At the weak point of above-mentioned present serum separating and extracting process, the objective of the invention is to propose a kind of novel serum separation method and device, this device is made of blood entrance, serum separating column, serum outlet and serum collection tube.
Serum separation method provided by the present invention and device, the material of described serum separating column is glass, polycarbonate, polymethylmethacrylate etc., inside is filled with the microballoon in different apertures.
Serum separation method provided by the present invention and device, described micro-sphere material can be high molecular polymers such as polycarbonate, tygon, also can be mineral compounds such as glass, silicon dioxide.Microspherulite diameter is 10～200 microns, can be the microballoon of single particle size, also can be the microballoon combination of different-grain diameter.
Serum separation method provided by the present invention and device, the size of described serum outlet are less than the size of microballon, and namely microballoon is jammed in the serum outlet.For example, when the microballon particle diameter of employed maximum was 150 microns, the serum outlet diameter can be 120 microns.
Serum separation method provided by the present invention and device, its principle is: whole blood is added from injection port, and blood sample is at gravity or certain clear separating column of the dirty menses of suction function, and haemocyte is trapped, serum then flows out from the hole between microballon, enters the serum collection tube at last.
Adopt serum separation method provided by the present invention and device to carry out the extraction of serum, equipment is simple, easy and simple to handle, separation efficiency is high.
Description of drawings
Fig. 1. the structural representation of a kind of serum tripping device provided by the invention.Wherein, A is injection port; B is the serum separating column; C is the serum outlet; D is the serum collection tube; E is the small particle diameter microballon; The big particle diameter microballon of F.
Specific embodiments
The following examples will give further instruction to the present invention in conjunction with Figure of description.
A kind of serum tripping device of embodiment
As shown in Figure 1, a kind of serum separating chips provided by the invention, injection port A, separating column B, serum outlet C, serum collection tube D form, and wherein are filled with the glass microballoon of a kind of (particle diameter is 100 μ m) or two kinds of particle diameters (particle diameter is respectively 20 μ m and 100 μ m) in the serum separating column.The serum separating step is as follows: whole blood is added from injection port, and blood sample is at gravity or certain clear separating column of the dirty menses of suction function, and haemocyte is trapped, and serum then flows out from the hole between microballon, enters the serum collection tube at last.Use this serum tripping device, can in 2min, be prepared into the blood serum sample of about 5 μ L.
Adopt serum separation method provided by the present invention and device to carry out the extraction of serum, equipment is simple, easy and simple to handle, separation efficiency is high.And this device can integrate with follow-up biochemistry detection analysis at an easy rate, realizes full-automatic, high-throughout biochemistry detection, has extremely important practical value.
Claims (7)
1. a serum separation method and device is characterized in that, this device is made of blood entrance, serum separating column, serum outlet and serum collection tube.
2. according to the described a kind of serum separation method of claim 1 and device, it is characterized in that the material of described serum separating column is glass, polycarbonate, polymethylmethacrylate etc., inside is filled with the microballoon in different apertures.
3. according to claim 1 and 2 described a kind of serum separation method and devices, it is characterized in that described micro-sphere material can be high molecular polymers such as polycarbonate, tygon, also can be mineral compounds such as glass, silicon dioxide.
4. according to claim 1 and 2 described a kind of serum separation method and devices, it is characterized in that described microspherulite diameter is 10～200 microns, can be the microballoon of single particle size, also can be the microballoon combination of different-grain diameter.
5. according to the described a kind of serum separation method of claim 1 and device, it is characterized in that the size of described serum outlet is less than the size of microballoon, namely microballoon is jammed in the serum outlet.
6. according to the described a kind of serum separation method of claim 1 and device, it is characterized in that, described serum separation method, its principle is: whole blood is added from injection port, blood sample is at gravity or certain clear separating column of the dirty menses of suction function, haemocyte is trapped, and serum then flows out from the hole between microballon, enters the serum collection tube at last.
7. according to the described a kind of serum separation method of claim 1 and device, it is characterized in that adopt method and apparatus of the present invention to carry out the extraction of serum, equipment is simple, easy and simple to handle, separation efficiency is high.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102036696A CN103293049A (en) | 2013-05-27 | 2013-05-27 | Serum separation method and serum separation device |
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| CN2013102036696A CN103293049A (en) | 2013-05-27 | 2013-05-27 | Serum separation method and serum separation device |
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| CN103293049A true CN103293049A (en) | 2013-09-11 |
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| CN2013102036696A Pending CN103293049A (en) | 2013-05-27 | 2013-05-27 | Serum separation method and serum separation device |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103604684A (en) * | 2013-11-22 | 2014-02-26 | 北京晶泰美康生物科技有限公司 | Sample separating device and sample separating method |
| CN105675379A (en) * | 2015-12-30 | 2016-06-15 | 南京工业大学 | Blood synchronously separating and detecting device |
| CN108362543A (en) * | 2018-02-08 | 2018-08-03 | 上海蓝怡科技股份有限公司 | A kind of serum separator and the method using its progress serum separation |
| CN109806632A (en) * | 2019-03-01 | 2019-05-28 | 中国科学院武汉物理与数学研究所 | A kind of Whole Blood Filtration device |
| CN110146567A (en) * | 2018-11-05 | 2019-08-20 | 浙江大学山东工业技术研究院 | A kind of anti-interference screen printing electrode based on microballoon |
| WO2022166230A1 (en) * | 2021-02-03 | 2022-08-11 | 杭州欣捷生物技术有限公司 | Composition and method for rapid separation of whole blood |
Citations (5)
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| CN1281145A (en) * | 2000-08-08 | 2001-01-24 | 湖北医科大学 | Blood separating colloid |
| CN1714292A (en) * | 2002-11-19 | 2005-12-28 | 积水化学工业株式会社 | Plasma or serum separation membrane and filter apparatus including the plasma or serum separation membrane |
| CN1920520A (en) * | 2006-09-13 | 2007-02-28 | 北京热景生物技术有限公司 | Pre-mounted eccentric column for detecting liver cancer alpha-fetoprotein heteroplasmon and reagent kid containing same |
| CN101189515A (en) * | 2005-06-27 | 2008-05-28 | 积水化学工业株式会社 | Instrument for separating blood and apparatus for separting blood |
| CN102788725A (en) * | 2012-07-29 | 2012-11-21 | 宁波市鄞州青林医疗器械技术咨询有限公司 | Serum separating tube with selective sucking function |
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2013
- 2013-05-27 CN CN2013102036696A patent/CN103293049A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1281145A (en) * | 2000-08-08 | 2001-01-24 | 湖北医科大学 | Blood separating colloid |
| CN1714292A (en) * | 2002-11-19 | 2005-12-28 | 积水化学工业株式会社 | Plasma or serum separation membrane and filter apparatus including the plasma or serum separation membrane |
| CN101189515A (en) * | 2005-06-27 | 2008-05-28 | 积水化学工业株式会社 | Instrument for separating blood and apparatus for separting blood |
| CN1920520A (en) * | 2006-09-13 | 2007-02-28 | 北京热景生物技术有限公司 | Pre-mounted eccentric column for detecting liver cancer alpha-fetoprotein heteroplasmon and reagent kid containing same |
| CN102788725A (en) * | 2012-07-29 | 2012-11-21 | 宁波市鄞州青林医疗器械技术咨询有限公司 | Serum separating tube with selective sucking function |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103604684A (en) * | 2013-11-22 | 2014-02-26 | 北京晶泰美康生物科技有限公司 | Sample separating device and sample separating method |
| CN103604684B (en) * | 2013-11-22 | 2016-03-02 | 北京纳晶生物科技有限公司 | Sample separating apparatus and method |
| CN105675379A (en) * | 2015-12-30 | 2016-06-15 | 南京工业大学 | Blood synchronously separating and detecting device |
| CN105675379B (en) * | 2015-12-30 | 2018-11-13 | 南京工业大学 | A kind of blood separated in synchronization verifying attachment |
| CN108362543A (en) * | 2018-02-08 | 2018-08-03 | 上海蓝怡科技股份有限公司 | A kind of serum separator and the method using its progress serum separation |
| CN110146567A (en) * | 2018-11-05 | 2019-08-20 | 浙江大学山东工业技术研究院 | A kind of anti-interference screen printing electrode based on microballoon |
| CN109806632A (en) * | 2019-03-01 | 2019-05-28 | 中国科学院武汉物理与数学研究所 | A kind of Whole Blood Filtration device |
| WO2022166230A1 (en) * | 2021-02-03 | 2022-08-11 | 杭州欣捷生物技术有限公司 | Composition and method for rapid separation of whole blood |
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Application publication date: 20130911 |