CN202022936U - Sealed fermentation tank inoculating system - Google Patents
Sealed fermentation tank inoculating system Download PDFInfo
- Publication number
- CN202022936U CN202022936U CN 201120081412 CN201120081412U CN202022936U CN 202022936 U CN202022936 U CN 202022936U CN 201120081412 CN201120081412 CN 201120081412 CN 201120081412 U CN201120081412 U CN 201120081412U CN 202022936 U CN202022936 U CN 202022936U
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- Prior art keywords
- fermentor tank
- fermentation tank
- steel cylinder
- buckle flange
- closed
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
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- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the field of chemical engineering, biological engineering and pharmaceutical engineering, and discloses a sealed fermentation tank inoculating system. The system comprises an accommodating steel bottle, a fermentation tank and a sealed connecting unit connecting the accommodating steel bottle with the fermentation tank. According to the system, the accommodating steel bottle and the fermentation tank are connected through the sealed connecting unit which is formed by butting a clamp flange against a ball valve, so as to avoid the fermentation tank and strains exposed in air during the inoculating process and inoculate the strain liquor to the fermentation tank through gravity; a vapor system is arranged on the connecting unit for disinfection, so as to achieve the sterile connection between the accommodating steel bottle and the fermentation tank; and the inoculating system has the advantage of flexibility in detachment, and sterile operations can be carried out on an operating console of a laboratory.
Description
Technical field
The utility model relates to chemical engineering, biotechnology, pharmaceutical engineering field, particularly relates to a kind of closed fermentor tank inoculation system.
Background technology
In fields such as chemical engineering, biotechnology, pharmaceutical engineering, industrial fermentation microbiological contamination meeting causes enormous economic loss, cause the factor of microbiological contamination varied, wherein the seeded process microbiological contamination is a principal element, data from the Streptomycin sulphate fermentation microbiological contamination analysis of causes of the 4th pharmaceutical factory shows that inoculation causes microbiological contamination to account for 11% in all microbiological contamination reasons.Inoculation method commonly used is a flame circle method in the fermentation industry field at present.Before the inoculation, use alcohol swab wiping seeding tank inoculation mouth and near zone, close the wind regime that can cause air turbulence as far as possible,, reduce tank pressure, close stirring as door, window, air-conditioning etc.Around the inoculation mouth, the alcohol swab circle is set, after being ready to bacterial classification suspension, light the alcohol swab circle, burn about 15 seconds, slowly open the inoculation mouth with spanner, another operator opens the inoculation bottleneck simultaneously, is fully burning about mouthful 10 seconds on the flame, remove inoculation mouthful nut afterwards, rapidly bacterial classification suspension is poured in the seeding tank.Cover inoculation mouthful and screw, the alcohol swab circle is taken off in the water extinguish, open stirring, adjust tank pressure, inoculation finishes.This vaccination ways shortcoming has three: one, and the step complexity lacks wherein one, causes easily promptly that all that has been achieved is spoiled; The 2nd, open system, bacterial classification and fermentor tank all need experience the step that is exposed to air, are easy to cause microbiological contamination; The 3rd, there is disaster hidden-trouble, use naked light in the production plant, there is disaster hidden-trouble.
The utility model content
(1) technical problem that will solve
The technical problems to be solved in the utility model is the microbiological contamination probability that how to reduce in the fermentation seeded process.
(2) technical scheme
In order to solve the problems of the technologies described above, the utility model provides a kind of closed fermentor tank inoculation system, and it comprises: contain kind of a steel cylinder, fermentor tank, and the sealing connector element that connects described Sheng kind steel cylinder and fermentor tank.
In the above-mentioned closed fermentor tank inoculation system, described sealing connector element comprises: the first buckle flange that docks with described Sheng kind steel cylinder docking port, the ball valve that docks with the described first buckle flange, and the second buckle flange that docks with the docking port of described ball valve and described fermentor tank respectively.
In the above-mentioned closed fermentor tank inoculation system, be provided with by-pass valve control between described second buckle flange and the described fermentor tank and near described fermentor tank one side.
In the above-mentioned closed fermentor tank inoculation system, be provided with steam unit between described second buckle flange and the described by-pass valve control.
In the above-mentioned closed fermentor tank inoculation system, described steam unit comprises vapour passage, and is arranged on the described vapour passage and is positioned at the admission control valve of described connector element both sides and goes out the vapour control valve.
In the above-mentioned closed fermentor tank inoculation system, described vapour passage is perpendicular to the central axis of described connector element.
In the above-mentioned closed fermentor tank inoculation system, the described first buckle flange and the second buckle flange dock by the docking port of silica gel pad with described Sheng kind steel cylinder and fermentor tank respectively.
In the above-mentioned closed fermentor tank inoculation system, described Sheng kind steel cylinder is the stainless steel inoculation bottle.
(3) beneficial effect
In the closed fermentor tank inoculation system that technique scheme provided, adopt connector element to be connected containing kind of steel cylinder with fermentor tank by the sealing that butt joint forms with ball valve of buckle flange, avoid that fermentor tank and bacterial classification are exposed to airborne situation in the seeded process, seed liquor is inoculated in the fermentor tank by gravity; And on connector element, be provided with steam system and carry out disinfection, thereby realized containing the aseptic connection of planting between steel cylinder and the fermentor tank; Inoculation system has the advantage of flexible dismounting simultaneously, can carry out aseptic technique in the laboratory operation platform.
Description of drawings
Fig. 1 is the structural representation of the closed fermentor tank inoculation system of the utility model embodiment.
Wherein, 1: contain kind of a steel cylinder; 2: the first buckle flanges; 3: ball valve; 4: the second buckle flanges; 5: the admission control valve; 6: go out the vapour control valve; 7: vapour passage; 8: by-pass valve control; 9: fermentor tank.
Embodiment
Below in conjunction with drawings and Examples, embodiment of the present utility model is described in further detail.Following examples are used to illustrate the utility model, but are not used for limiting scope of the present utility model.
Fig. 1 shows the structural representation of the closed fermentor tank inoculation system of the utility model embodiment, and this system comprises contains kind of a steel cylinder 1, fermentor tank 9, and connects the sealing connector element of containing kind of steel cylinder 1 and fermentor tank 9; The sealing connector element will contain the docking port of kind of steel cylinder 1 and the docking port of fermentor tank 9 couples together, and the seed liquor that realizes containing in kind of the steel cylinder 1 is planted a steel cylinder 1 and flowed in the fermentor tank 9 and ferments by containing.
As shown in Figure 1, the sealing connector element specifically comprises: dock the first buckle flange 2, the ball valve 3 that docks with the first buckle flange 2, and the second buckle flange 4 that docks with the docking port of ball valve 3 and fermentor tank 9 respectively with Sheng kind of steel cylinder 1 docking port; Also be provided with by-pass valve control 8 between the second buckle flange 4 and the fermentor tank 9 and near fermentor tank 9 one sides.In addition, in order further to guarantee the sterility of sealing connected system, on the sealing connector element, also be provided with steam unit, connector element is carried out steam sterilizing, steam unit specifically is arranged between the second buckle flange 4 and the by-pass valve control 8, steam unit comprises vapour passage 7, and be arranged on the vapour passage 7 and be positioned at the admission control valve 5 of connector element both sides and go out vapour control valve 6, vapour passage 7 is perpendicular to the central axis of connector element, so both made things convenient for steam unit that connector element is carried out disinfection, also can not flow to the seed liquor in the connector element has a negative impact.
The repeated inoculation of system uses for convenience, to be set to removably connect between connector element and Sheng kind of steel cylinder 1 and the fermentor tank 9, particularly, the first buckle flange 2 and the second buckle flange 4 are respectively by silica gel pad and provisional butt joint of docking port of containing kind of steel cylinder 1 and fermentor tank 9; Wherein contain kind of a steel cylinder 1 and be preferably stainless steel inoculation steel cylinder, conveniently carry out disinfection and recycle containing kind of a steel cylinder 1.
When utilizing above-mentioned closed fermentor tank inoculation system to carry out the seed liquor inoculation, at first contain kind of a steel cylinder 1, the first buckle flange 2, ball valve 3, the second buckle flange 4 docks successively and is one, contain ready seed liquor in containing kind of steel cylinder 1, ball valve 3 is in closing condition, and the second buckle flange, 4 one sides are with gauze and kraft paper parcel; Then gauze and kraft paper are removed, the second buckle flange, 4 one sides and fermentor tank 9 one sides are fitted together, open admission control valve 5 and go out vapour control valve 6, with saturation steam sterilization 30 minutes; Close out vapour control valve 6 and admission control valve 5 afterwards successively, open by-pass valve control 8 and ball valve 3, seed liquor enters fermentor tank 9 under action of gravity.After inoculation finishes, closing control valve 8 and ball valve 3, order is opened admission control valve 5 and is gone out vapour control valve 6, with saturation steam sterilization 30 minutes, close out vapour control valve 6 and admission control valve 5 after finishing successively, and from the second buckle flange, 4 places inoculation system is taken off, decompose, clean, sterilize, prepare next time and use.
As can be seen from the above embodiments, the utility model embodiment adopts the connector element by the sealing that butt joint forms with ball valve of buckle flange to be connected by containing kind of steel cylinder with fermentor tank, avoid that fermentor tank and bacterial classification are exposed to airborne situation in the seeded process, seed liquor is inoculated in the fermentor tank by gravity; And on connector element, be provided with steam system and carry out disinfection, thereby realized containing the aseptic connection of planting between steel cylinder and the fermentor tank; Inoculation system has the advantage of flexible dismounting simultaneously, can carry out aseptic technique in the laboratory operation platform.
The above only is a preferred implementation of the present utility model; should be understood that; for those skilled in the art; under the prerequisite that does not break away from the utility model know-why; can also make some improvement and replacement, these improvement and replacement also should be considered as protection domain of the present utility model.
Claims (8)
1. a closed fermentor tank inoculation system is characterized in that, comprising: contain kind of a steel cylinder, fermentor tank, and the sealing connector element that connects described Sheng kind steel cylinder and fermentor tank.
2. closed fermentor tank inoculation system as claimed in claim 1, it is characterized in that, described sealing connector element comprises: the first buckle flange that docks with described Sheng kind steel cylinder docking port, the ball valve that docks with the described first buckle flange, and the second buckle flange that docks with the docking port of described ball valve and described fermentor tank respectively.
3. closed fermentor tank inoculation system as claimed in claim 2 is characterized in that, is provided with by-pass valve control between described second buckle flange and the described fermentor tank and near described fermentor tank one side.
4. closed fermentor tank inoculation system as claimed in claim 3 is characterized in that, is provided with steam unit between described second buckle flange and the described by-pass valve control.
5. closed fermentor tank inoculation system as claimed in claim 4 is characterized in that described steam unit comprises vapour passage, and is arranged on the described vapour passage and is positioned at the admission control valve of described connector element both sides and goes out the vapour control valve.
6. closed fermentor tank inoculation system as claimed in claim 5 is characterized in that described vapour passage is perpendicular to the central axis of described connector element.
7. closed fermentor tank inoculation system as claimed in claim 2 is characterized in that, the described first buckle flange and the second buckle flange dock by the docking port of silica gel pad with described Sheng kind steel cylinder and fermentor tank respectively.
8. closed fermentor tank inoculation system as claimed in claim 1 is characterized in that, described Sheng kind steel cylinder is the stainless steel inoculation bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120081412 CN202022936U (en) | 2011-03-24 | 2011-03-24 | Sealed fermentation tank inoculating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120081412 CN202022936U (en) | 2011-03-24 | 2011-03-24 | Sealed fermentation tank inoculating system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202022936U true CN202022936U (en) | 2011-11-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120081412 Expired - Lifetime CN202022936U (en) | 2011-03-24 | 2011-03-24 | Sealed fermentation tank inoculating system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202022936U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103382440A (en) * | 2013-06-24 | 2013-11-06 | 江苏元洲生物工程有限公司 | Safe inoculation device of liquid fermentation tank |
| WO2021031680A1 (en) * | 2019-08-21 | 2021-02-25 | 浙江李子园食品股份有限公司 | Fermentation sterile inoculation device |
-
2011
- 2011-03-24 CN CN 201120081412 patent/CN202022936U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103382440A (en) * | 2013-06-24 | 2013-11-06 | 江苏元洲生物工程有限公司 | Safe inoculation device of liquid fermentation tank |
| WO2021031680A1 (en) * | 2019-08-21 | 2021-02-25 | 浙江李子园食品股份有限公司 | Fermentation sterile inoculation device |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20111102 |
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| CX01 | Expiry of patent term |