CN201908090U - Fixed bed platy carrier aseptic vacuum sampling device - Google Patents
Fixed bed platy carrier aseptic vacuum sampling device Download PDFInfo
- Publication number
- CN201908090U CN201908090U CN 201020617849 CN201020617849U CN201908090U CN 201908090 U CN201908090 U CN 201908090U CN 201020617849 CN201020617849 CN 201020617849 CN 201020617849 U CN201020617849 U CN 201020617849U CN 201908090 U CN201908090 U CN 201908090U
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- China
- Prior art keywords
- valve
- sampling
- fixed bed
- sterilization
- vacuum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to a fixed bed platy carrier aseptic vacuum sampling device, comprising a sampling valve connected with a fermentation tank, wherein the fermentation tank is provided with a flexible sampling pipe communicated with an internal cavity of the sampling valve; one end of the sampling valve is sequentially connected with a discharge valve and an L-shaped valve, and the L-shaped valve is simultaneously connected with a sterilization valve and a sampling bottle; the sterilization valve is connected with a stream trap, the other end of the sampling valve is connected with a sterile air inlet valve and a vacuumizing valve, and the vacuumizing valve is simultaneously connected with an on-line air inlet valve and a peristaltic pump which is connected with a waste gas collection bottle. The fixed bed plsaty carrier aseptic vacuum sampling device can conduct on-line sterilization on the sampling valve and the sampling pipeline, and adopts the peristaltic pump for vacuum suction to enable platy carriers in the fermentation tank to be totally isolated from outside air; and the platy carriers are extracted by the flexible sampling pipe through observation via a view window of the fermentation tank, are taken out smoothly from a fixed bed and finally enter the sampling bottle to realize the safe pollution-free sampling of the platy carriers.
Description
Technical field
The utility model relates to the sampling unit of cell cultivation equipment, specifically is a kind of fixed bed chip carrier sterile sampling device.
Background technology
At present, as cell cultivation equipment, enjoy the favor of bio-pharmaceuticals industry by the sheet-like fiber carrier fixed bed pattern cell cultures jar of U.S. NBS company release.But its sampling to the chip carrier cell only is the sampling of glass tubular type at present, and this sampling is very dangerous, and can't directly measure and observe stand density and the upgrowth situation that is adsorbed on cell on the chip carrier.
Summary of the invention
The purpose of this utility model is to provide a kind of fixed bed chip carrier sampling vacuum unit, takes a sample to realize the chip carrier in the fixed bed, and guarantees that whole sampling process is safe and reliable free from environmental pollution.
The aseptic vacuum sampling device of this fixed bed chip carrier comprise with the fermentation tank connected sampling valve, fermentor tank be provided with communicate with the sampling valve inner chamber around the property stopple coupon; One end of sampling valve connects baiting valve and L type valve successively, and L type valve is connected with sampling jug with the sterilization valve simultaneously; The sterilization valve is connected with trap, and the other end of sampling valve connects sterile air intake valve and vacuum pumping valve, and vacuum pumping valve connects online intake valve and peristaltic pump simultaneously, and peristaltic pump links to each other with the gas sampling bottle.
Be connected with flowrate control valve between sampling valve and the sterile air intake valve, vacuum pressure gauge is housed on the flowrate control valve.
Around the property stopple coupon universal rocking bar is housed, can in fermentor tank, moving catches chip carrier by universal rocking bar control around the property stopple coupon.
Sampling valve is provided with viewing window.
This device can carry out online sterilization to sampling valve and sampling line, adopts the peristaltic pump vacuum take-off, and chip carrier and outside air in the fermentor tank are isolated fully.Adopting the universal rocking bar of health level to drive around the property stopple coupon moves in fermentor tank, observe by the fermentor tank form simultaneously, make around the property stopple coupon and aim at chip carrier, extract chip carrier to sampling valve, successfully in fixed bed, take out and enter at last sampling jug, realize the safety non-pollution sampling of chip carrier.After finishing sampling, sampling jug is taken off censorship together with the L type valve integral body that is attached thereto.
The aseptic vacuum sampling device of fixed bed chip carrier of the present utility model is simple in structure, can be installed on the stainless steel bio-reactor of any size, safe and reliable, easy to operate, repetitive operation as required, at any time take out an amount of chip carrier, and can realize self online sterilization, avoid bacteriophage that human body and environment are caused danger such as soaking evil.
Description of drawings
Fig. 1 is an one-piece construction synoptic diagram of the present utility model.
Among Fig. 1: the 1st, sampling jug, the 2nd, sampling sebific duct, the 3rd, fermentor tank, the 4th, around the property stopple coupon, the 5th, L type valve, the 6th, baiting valve, the 7th, universal rocking bar, the 8th, sampling valve, the 9th, vacuum pressure gauge, the 10th, flowrate control valve, the 11st, aseptic vacuum-operated inlet valve, the 12nd, vacuum pumping valve, the 13rd, online sterilization intake valve, the 14th, peristaltic pump, the 15th, exhausting waste gas sebific duct, the 16th, sterilization valve and 17 is gas sampling bottles, the 18th, trap.
Embodiment
Below in conjunction with description of drawings structure of the present utility model and working process.
Sampling valve 8 is connected with fermentor tank 3.Fermentor tank 3 is provided with around property stopple coupon 4, around the property stopple coupon the universal rocking bar 7 of health level is housed, and 7 controls of universal rocking bar are controlled universal rocking bar 7 around property stopple coupon 4, can make to pounce on around property stopple coupon 4 moving in fermentor tank 3 to catch chip carrier.The lower end of sampling valve 8 connects baiting valve 6 and L type valve 5 successively, and L type valve 5 also is connected with sterilization valve 16 simultaneously, and sterilization valve 16 is connected with trap 18, is connected with sampling jug 1 by sampling sebific duct 2; Upper end connection traffic control valve 10, sterile air intake valve 11 and the vacuum pumping valve 12 of sampling valve 8, flowrate control valve 10 is equipped with vacuum pressure gauge 9; Vacuum pumping valve 12 connects online sterilization intake valve 13 and peristaltic pump 14 simultaneously, and peristaltic pump 14 links to each other with gas sampling bottle 17 by exhausting waste gas sebific duct 15.Sterilization valve 16 links to each other with trap 18.Above-mentioned various valve all is in closing condition, should install at the aseptic technique platform in advance through the sampling jug 1 of washing and sterilizing, the sebific duct 2 of taking a sample, L type valve.
This device working process is as follows:
1, sampling valve 8 and fermentor tank 3 carry out online sterilization simultaneously.
During the online sterilization of fermentor tank 3, open sampling valve 8, baiting valve 6, sterilization valve 16, carry out sampling valve 8 and fermentor tank 3 and carry out online sterilization simultaneously, close sampling valve 8 subsequently;
2, carry out the online sterilization of sampling line
Open flowrate control valve 10, baiting valve 6, sterilization valve 16, slowly opening online sterilization intake valve 13(control vapor pressure at last is 1.2 crust), carry out the online sterilization of sampling line.Close flowrate control valve 10, baiting valve 6 after finishing, sterilization valve 16 and online sterilization intake valve 13;
3, sampling
A opens sterile air intake valve 11, slowly open flowrate control valve 10, make the pressure of sampling valve 8 inner air pressures a little more than fermentor tank 3, close sterile air intake valve 11 and flowrate control valve 10 then, slowly open sampling valve 8 and head light thereof, the pressure in sampling valve 8 and the fermentor tank 3 is balanced each other;
B starts about peristaltic pump 14(100r/min) vacuumize, open vacuum pumping valve 12, the body of bleeding this moment is got rid of through gas sampling bottle 17.The universal rocking bar 7 of swing health level makes around property stopple coupon 4 and aims at the chip carrier that will extract, and slowly turn-on flow rate control valve 10 can be observed the taking-up situation of chip carrier at the viewing window of sampling valve 8 with control extraction speed.
C extracts out if any chip carrier, close vacuum pumping valve 12 and peristaltic pump 14 stops to vacuumize, open baiting valve 6 simultaneously and L type valve 5 is taken a sample, in the sampling valve 8 as stop when chip carrier is arranged, can slowly open sterile air intake valve 11, make the chip carrier that rests in the sampling valve 8 under the effect of sterile air, enter sampling jug 1, close sampling valve 8 then.
4 sterilizations
After sampling is finished, carry out the online sterilization of sampling valve 8, flowrate control valve 10, sterile air intake valve 7, vacuum pumping valve 12, baiting valve 6, L type valve 5, sterilization valve 16 and pipeline thereof earlier.Integral body is taken off vacuum pumping valve 12, exhausting waste gas sebific duct 15 and gas sampling bottle 17 and is sent into the sterilization of sterilization cabinet then.
5 censorships
Integral body is taken off L type valve 5, sampling sebific duct 2 and sampling jug 1 censorship.
Repeat 2,3,4,5 steps sub-sampling again.
Claims (5)
1. the aseptic vacuum sampling device of fixed bed chip carrier is characterized in that comprising and the tank connected sampling valve of fermentation, fermentor tank be provided with communicate with the sampling valve inner chamber around the property stopple coupon; One end of sampling valve connects baiting valve and L type valve successively, and L type valve is connected with sampling jug with the sterilization valve simultaneously; The sterilization valve is connected with trap, and the other end of sampling valve connects sterile air intake valve and vacuum pumping valve, and vacuum pumping valve connects online intake valve and peristaltic pump simultaneously, and peristaltic pump links to each other with the gas sampling bottle.
2. the aseptic vacuum sampling device of fixed bed chip carrier according to claim 1 is characterized in that around the property stopple coupon universal rocking bar being housed.
3. the aseptic vacuum sampling device of fixed bed chip carrier according to claim 1 is characterized in that being connected with flowrate control valve between described sampling valve and the sterile air intake valve.
4. the aseptic vacuum sampling device of fixed bed chip carrier according to claim 3 is characterized in that being equipped with on the flowrate control valve vacuum pressure gauge.
5. according to claim 1 or the aseptic vacuum sampling device of 2 or 3 or 4 described fixed bed chip carriers, it is characterized in that described sampling valve is provided with viewing window and head light.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201020617849 CN201908090U (en) | 2010-11-22 | 2010-11-22 | Fixed bed platy carrier aseptic vacuum sampling device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201020617849 CN201908090U (en) | 2010-11-22 | 2010-11-22 | Fixed bed platy carrier aseptic vacuum sampling device |
Publications (1)
Publication Number | Publication Date |
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CN201908090U true CN201908090U (en) | 2011-07-27 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN 201020617849 Expired - Fee Related CN201908090U (en) | 2010-11-22 | 2010-11-22 | Fixed bed platy carrier aseptic vacuum sampling device |
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CN (1) | CN201908090U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102698693A (en) * | 2012-05-31 | 2012-10-03 | 苏州市金翔钛设备有限公司 | Reaction kettle capable of sampling |
-
2010
- 2010-11-22 CN CN 201020617849 patent/CN201908090U/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102698693A (en) * | 2012-05-31 | 2012-10-03 | 苏州市金翔钛设备有限公司 | Reaction kettle capable of sampling |
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Legal Events
Date | Code | Title | Description |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110727 Termination date: 20181122 |