CN205313525U - Liquid spawn dona tub - Google Patents
Liquid spawn dona tub Download PDFInfo
- Publication number
- CN205313525U CN205313525U CN201620042724.7U CN201620042724U CN205313525U CN 205313525 U CN205313525 U CN 205313525U CN 201620042724 U CN201620042724 U CN 201620042724U CN 205313525 U CN205313525 U CN 205313525U
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- China
- Prior art keywords
- ball valve
- liquid spawn
- air
- tank
- accurate filter
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- Expired - Fee Related
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Abstract
The utility model discloses a liquid spawn dona tub has an inoculation opening at the tank deck to and sterilization steam flow path, air precision filter, air -purifying passageway and a exhaust outlet, physically there is an observation window in upper portion at the jar. The utility model discloses liquid spawn dona tub and the separation of liquid spawn fermentation cylinder, sterilization alone, inoculation, every liquid spawn dona tub all can vaccinate different liquid spawn fermentation cylinders. The specific pipeline of liquid spawn dona tub is conveniently sterilized, is ventilated, is inserted bacterial, sampling and operates the reducing pollutants probability to each items such as liquid spawn fermentation cylinder inoculations, simultaneously, there is fragmentation action to the fungus ball, when vaccinating the liquid spawn fermentation cylinder, produces a large amount of hypha pieces, be favorable to the mycelium to be bred rapidly in the liquid spawn fermentation cylinder.
Description
Technical field
This utility model relates to the equipment of a kind of yeast culture and inoculation, is specifically related to a kind of liquid spawn seed tank, belongs to microorganism culturing Preparation equipment field.
Background technology
At present, utilize seed tank culture strain is substantially prior art in this area. But current prior art, complex operation when cultivating and inoculate, it is easy to produce antibacterial and infect, cause failure.
Utility model content
One of the purpose of this utility model is in that to provide a kind of liquid spawn seed tank.
Liquid spawn seed tank, has an inoculation mouth 5 at tank deck, and sterilizing steam channel 1, air accurate filter 2, purifies air duct 3 and waste gas outlet 4, has an observation window 6 on tank body top, described sterilizing steam channel includes a steam inlet K1 connected with tank interior, steam inlet K1 has ball valve F2, a lateral is had between ball valve F2 and steam inlet K1 entrance, lateral has ball valve F1, it is connected with air accurate filter by pipeline at steam inlet K1, air accurate filter 2 has Pressure gauge B1, pipeline between steam inlet K1 and air accurate filter 2 has ball valve F3, pipeline between ball valve F3 with air accurate filter 2 is connected ball valve F4 and air intlet K2 by a lateral, air accurate filter 2 is connected with ball valve F5, check-valves Z1 is had between air accurate filter 2 and purification air duct 3, purify order on air duct 3 and ball valve F6 and ball valve F8 is installed, purify air duct 3 to connect with tank interior, it is connected to ball valve F7 by a lateral between ball valve F6 and ball valve F8, waste gas outlet pipeline is arranged on tank deck, waste gas outlet pipeline is separately installed with Pressure gauge B2, ball valve F9, the check-valves Z2 being connected by a lateral between Pressure gauge B2 with ball valve F9 and relief valve A1.
Liquid spawn seed tank of the present utility model, rational in infrastructure, can avoid polluting when spawn culture and inoculation by high degree. The specific pipeline of liquid spawn seed tank, has fragmentation to fungus ball, when liquid spawn fermentation tank is inoculated, produces a large amount of mycelia fragment, is conducive to mycelium to breed rapidly in liquid spawn fermentation tank.
This utility model in use, comprises the steps:
Step one, air sterilization air accurate filter carry out sterilizing: ball valve F1, ball valve F2, ball valve F4, ball valve F7 close, ball valve F3, ball valve F6, ball valve F9 are logical, steam inlet K1 connects high-temperature steam, ball valve F5 gives vent to anger draining slightly, regulating ball valve F8 makes Pressure gauge B1 stable at 0.14MPa, keeps 30 minutes.
Step 2, air sterilization air accurate filter dry up: close ball valve F3, air intlet K2 connects pure air, pass ball valve F4, ball valve F7, ball valve F8 closes after ventilating 1 minute and (prevents from forming vacuum, pipeline sucks culture fluid and causes that sterilizing is not thorough), filter element is dried up, until liquid spawn seed tank sterilizing terminates.
Step 3, liquid spawn seed tank culture liquid carry out sterilizing: pass ball valve F2, and after cultivating boiling, ball valve F1 ventilates slightly, regulate ball valve F9 and make Pressure gauge B2 stable at 0.14MPa, keep 40 minutes, close ball valve F1, ball valve F2. Air sterilization air accurate filter dries up and carries out sterilizing with liquid spawn seed tank culture liquid and carry out simultaneously.
Step 4, liquid spawn seed tank pass into air: when Pressure gauge B2 shows about 0.05MPa, close ball valve F5, ball valve F7, pass ball valve F8, regulate ball valve F9 and make Pressure gauge B2 stable at 0.1MPa.
Step 5, access strain: when culture-liquid temp drops to about 25 degree, with pyrosphere protection inoculation mouth, access triangular flask strain.
Step 6, sampling detection: the previous day preparing that liquid spawn fermentation tank is inoculated is sampled detection, closes ball valve F6, ball valve F9, under the protection of flame; slightly opening ball valve F7, liquid spawn is flowed out automatically, completes sampling; after sampling terminates, valve returns to the state before sampling. Detection sampling, as pollution-free, namely liquid spawn seed tank can be used for liquid spawn fermentation tank inoculation.
Step 7, to liquid spawn fermentation tank inoculate: during liquid spawn fermentation tank sterilizing, liquid spawn fermentation tank top strain access port and liquid spawn seed tank steam inlet K1 is connected with silica gel tube, open liquid spawn fermentation tank strain access port valve, regulate ball valve F1, make its draining of slightly giving vent to anger, until liquid spawn fermentation tank sterilizing terminates, close ball valve F1. Inoculating after liquid spawn fermentation tank cooling, close ball valve F9, pass ball valve F2, in liquid spawn seed tank, liquid spawn is all automatically injected liquid spawn fermentation tank, closing liquid strain fermentation tank top strain access port by silica gel tube, completes inoculation work.
This liquid spawn seed tank is suitable for the liquid spawn fermentation tank to 200-5000L and inoculates.
Volume is the 5% of liquid spawn fermentation tank.
It is said that in general, this liquid spawn seed tank needs following condition when using: 1, there is the air source that oil-free is clean. 2, there is the vapour source of more than pressure 0.2MPa. 3, there is air-conditioning indoor.
This utility model has the advantage that
1, liquid spawn seed tank separates with liquid spawn fermentation tank, independent sterilizing, inoculation, and each liquid spawn seed tank all can inoculate different liquid spawn fermentation tanks. If liquid spawn seed tank pollutes, do not affect liquid spawn fermentation tank and normally use, lose less.
2, before liquid spawn fermentation tank sterilizing, liquid spawn fermentation tank and liquid spawn seed tank is connected with silica gel tube, during liquid spawn fermentation tank sterilizing, inoculation pipeline is carried out sterilizing simultaneously, seeded process contamination probability is zero, liquid spawn seed tank is such as pollution-free, then liquid spawn fermentation tank success ratio of inoculation 100%.
3, innovation pipeline connects, and sterilizing steam inlet doubles as strain outlet, purifies air duct and doubles as thief hatch, pipeline is unique succinct, easy to operate, without dead angle, it is convenient to complete sterilizing, ventilation, access strain, sampling and to operations such as liquid spawn fermentation tank inoculations. Strain outlet splits with thief hatch, can avoid thief hatch just in case polluting the impact on seeded process.
4, the specific pipeline of liquid spawn seed tank, has fragmentation to fungus ball, when liquid spawn fermentation tank is inoculated, produces a large amount of mycelia fragment, is conducive to mycelium to breed rapidly in liquid spawn fermentation tank.
Accompanying drawing explanation
Fig. 1 is the overall schematic of this utility model liquid spawn seed tank;
Fig. 2 is the profile of this utility model liquid spawn seed tank;
Fig. 3 is the structural representation of this utility model liquid spawn seed tank capping.
Detailed description of the invention
Below in conjunction with accompanying drawing, this utility model is further described.
As shown in accompanying drawing 1,2,3, liquid spawn seed tank, there is an inoculation mouth 5 at tank deck, and sterilizing steam channel 1, air accurate filter 2, purify air duct 3 and waste gas outlet 4, have an observation window 6 on tank body top, described sterilizing steam channel includes a steam inlet K1 connected with tank interior, steam inlet K1 has ball valve F2, a lateral is had between ball valve F2 and steam inlet K1 entrance, lateral has ball valve F1, it is connected with air accurate filter by pipeline at steam inlet K1, air accurate filter 2 has Pressure gauge B1, pipeline between steam inlet K1 and air accurate filter 2 has ball valve F3, pipeline between ball valve F3 with air accurate filter 2 is connected ball valve F4 and air intlet K2 by a lateral, air accurate filter 2 is connected with ball valve F5, check-valves Z1 is had between air accurate filter 2 and purification air duct 3, purify order on air duct 3 and ball valve F6 and ball valve F8 is installed, purify air duct 3 to connect with tank interior, it is connected to ball valve F7 by a lateral between ball valve F6 and ball valve F8, waste gas outlet pipeline is arranged on tank deck, waste gas outlet pipeline is separately installed with Pressure gauge B2, ball valve F9, the check-valves Z2 being connected by a lateral between Pressure gauge B2 with ball valve F9 and relief valve A1.
The using method of liquid spawn seed tank, specifically includes following steps:
Step one, air sterilization air accurate filter carry out sterilizing: ball valve F1, ball valve F2, ball valve F4, ball valve F7 close, ball valve F3, ball valve F6, ball valve F9 are logical, steam inlet K1 connects high-temperature steam, ball valve F5 gives vent to anger draining slightly, regulating ball valve F8 makes Pressure gauge B1 stable at 0.14MPa, keeps 30 minutes.
Step 2, air sterilization air accurate filter dry up: close ball valve F3, air intlet K2 connects pure air, pass ball valve F4, ball valve F7, ball valve F8 closes after ventilating 1 minute and (prevents from forming vacuum, pipeline sucks culture fluid and causes that sterilizing is not thorough), filter element is dried up, until liquid spawn seed tank sterilizing terminates.
Step 3, liquid spawn seed tank culture liquid carry out sterilizing: pass ball valve F2, and after cultivating boiling, ball valve F1 ventilates slightly, regulate ball valve F9 and make Pressure gauge B2 stable at 0.14MPa, keep 40 minutes, close ball valve F1, ball valve F2. Air sterilization air accurate filter dries up and carries out sterilizing with liquid spawn seed tank culture liquid and carry out simultaneously.
Step 4, liquid spawn seed tank pass into air: when Pressure gauge B2 shows about 0.05MPa, close ball valve F5, ball valve F7, pass ball valve F8, regulate ball valve F9 and make Pressure gauge B2 stable at 0.1MPa.
Step 5, access strain: when culture-liquid temp drops to about 25 degree, with pyrosphere protection inoculation mouth, access triangular flask strain.
Step 6, sampling detection: the previous day preparing that liquid spawn fermentation tank is inoculated is sampled detection, closes ball valve F6, ball valve F9, under the protection of flame; slightly opening ball valve F7, liquid spawn is flowed out automatically, completes sampling; after sampling terminates, valve returns to the state before sampling. Detection sampling, as pollution-free, namely liquid spawn seed tank can be used for liquid spawn fermentation tank inoculation.
Step 7, to liquid spawn fermentation tank inoculate: during liquid spawn fermentation tank sterilizing, liquid spawn fermentation tank top strain access port and liquid spawn seed tank steam inlet K1 is connected with silica gel tube, open liquid spawn fermentation tank strain access port valve, regulate ball valve F1, make its draining of slightly giving vent to anger, until liquid spawn fermentation tank sterilizing terminates, close ball valve F1. Inoculating after liquid spawn fermentation tank cooling, close ball valve F9, pass ball valve F2, in liquid spawn seed tank, liquid spawn is all automatically injected liquid spawn fermentation tank, closing liquid strain fermentation tank top strain access port by silica gel tube, completes inoculation work.
Within 2015, this liquid spawn seed tank is through on probation, annual strain 166 tank that makes, success 158 tanks; Inoculation liquid spawn fermentation tank 133 tank, all success.
Claims (1)
1. liquid spawn seed tank, it is characterised in that: there is an inoculation mouth at tank deck, and sterilizing steam channel, air accurate filter, purify air duct and waste gas outlet, have an observation window on tank body top, described sterilizing steam channel includes a steam inlet K1 connected with tank interior, steam inlet K1 has ball valve F2, a lateral is had between ball valve F2 and steam inlet K1 entrance, lateral has ball valve F1, it is connected with air accurate filter by pipeline at steam inlet K1, air accurate filter has Pressure gauge B1, pipeline between steam inlet K1 and air accurate filter has ball valve F3, pipeline between ball valve F3 with air accurate filter is connected ball valve F4 and air intlet K2 by a lateral, air accurate filter is connected with ball valve F5, check-valves Z1 is had between air accurate filter and purification air duct, purify order on air duct and ball valve F6 and ball valve F8 is installed, purify air duct to connect with tank interior, it is connected to ball valve F7 by a lateral between ball valve F6 and ball valve F8, waste gas outlet pipeline is arranged on tank deck, waste gas outlet pipeline is separately installed with Pressure gauge B2, ball valve F9, the check-valves Z2 being connected by a lateral between Pressure gauge B2 with ball valve F9 and relief valve A1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620042724.7U CN205313525U (en) | 2016-01-07 | 2016-01-07 | Liquid spawn dona tub |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620042724.7U CN205313525U (en) | 2016-01-07 | 2016-01-07 | Liquid spawn dona tub |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205313525U true CN205313525U (en) | 2016-06-15 |
Family
ID=56202475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620042724.7U Expired - Fee Related CN205313525U (en) | 2016-01-07 | 2016-01-07 | Liquid spawn dona tub |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205313525U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505744A (en) * | 2016-01-07 | 2016-04-20 | 盐城市雨露农业科技有限公司 | Liquid strain seed tank and using method thereof |
-
2016
- 2016-01-07 CN CN201620042724.7U patent/CN205313525U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505744A (en) * | 2016-01-07 | 2016-04-20 | 盐城市雨露农业科技有限公司 | Liquid strain seed tank and using method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160615 Termination date: 20170107 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |