CN201593045U

CN201593045U - Simple gas gene gun

Info

Publication number: CN201593045U
Application number: CN2010200102854U
Authority: CN
Inventors: 王振东; 张良; 任大明; 王惠; 曹冬煦
Original assignee: Shenyang Agricultural University
Current assignee: Shenyang Agricultural University
Priority date: 2010-01-19
Filing date: 2010-01-19
Publication date: 2010-09-29
Anticipated expiration: 2020-01-19

Abstract

A simple gas gene gun comprises a gun body, a compression gas inlet pipe, a gas valve, an accelerating pipe and gas communication pipes. The gas valve is controlled by a trigger; and the gas communication pipes are arranged among all portions. The simple gas gene gun is characterized in that a magazine is arranged between the tail end of the accelerating pipe and the starting end of an ejection pipe; and a thin metal net is arranged inside the magazine along the cross section. The simple gas gene gun has the advantages of simple structure, convenient sample loading and accurate inoculation as samples are directly attached on the thin metal net in the magazine. Moreover, the simple gas gene gun has low price and an effect better than the effect of the prior art.

Description

A kind of simple type gas gene gun

Technical field

The utility model relates to a kind of device that in bio-genetics genetic material is injected in plant leaf, tissue or the cell, particularly a kind of simple type gas gene gun.

Background technology

Particle gun is the indispensable important tool of field of genetic engineering, initial particle gun is that the gas that adopts powder burning to produce bombards, it as the patent No. 89109334 the disclosed particle gun of Chinese utility application " a kind of transgenic method and genetically modified particle are robbed ", it utilizes " speed and the method for the proportion speed ground control bombardment of gunpowder; also can operate with task equipment; both can bombard the biomass cells that exsomatizes; also can bombard the substance biomass cells; realize the importing of foreign gene, thereby change biological proterties under closed state and non-closed state." this genoid rifle injures to some extent to the target sample, the impurity that produces of powder burning also can be injected in the sample with high velocity air simultaneously, and its precision also is difficult to control, and high-temperature gas is unfavorable for that also gene imports, expresses in addition.Thereby occurred utilizing high pressure gas to change the particle gun of the shockwave of generation afterwards as propulsion source, as U.S. Pat 5865796, disclosed among the US5584807 is exactly a kind of pistol type particle gun, its scheme includes handle, the pressurized gas inlet suction port, air valve, gun barrel, the magazine seat, and interior magazine and set ring, inlet suction port is positioned at handle bottom and is connected with air valve on being fixed on handle through handle gas inside passage, the air outlet of air valve is communicated with tail end gas passage at gun barrel, magazine seat and inner magazine thereof are arranged on the terminal position of gun barrel, set ring is connected the muzzle position of gun barrel, so that muzzle and target keep certain distance, during use, inlet suction port is connected high-pressure air source, when pressure is enough, open air valve, pressurized gas enters gun barrel rapidly and forms a shockwave, and the DNA particulate that is arranged in the magazine is entered target from the muzzle ejaculation; In this kind design, the magazine seat is the runner shape of revolver, be provided with a plurality of magazine holes that hold magazine in the circumferential arrangement mode, the magazine seat is provided with magazine hole corresponding positioning hole at its outer wall, pistol then is provided with a steady brace in corresponding position, both cooperatively interact make magazine seat location and make one of them magazine hole and gun barrel to directly, after shooting away a DNA particulate, make the magazine seat break away from steady brace, rotate an angle then and make next magazine hole and gun barrel to directly, again with the fixing magazine seat of steady brace.Its shortcoming is that the magazine seat only depends on the steady brace location, stable inadequately, if when a plurality of magazines are laid in the magazine seat simultaneously, the vibrations that a preceding fire is brought can influence as yet the not homogeneity of the DNA particulate of emission, serious meeting is polluted the DNA particulate, the operation of rotating the magazine seat simultaneously is also convenient inadequately, the pressurized gas that is used for launching the DNA particulate is on the other hand directly imported by source of the gas, do not pass through the gas storage stable-pressure device, so not only because of the gas circuit pressure-losses influences stable gas pressure, but also can influence the initial velocity of emission DNA particulate.

The utility model content

The purpose of this utility model is to provide a kind of simple type gas gene gun at the problems referred to above, and it is simple in structure, and sample is attached directly on the interior fine metal mesh of magazine, and application of sample is convenient, and inoculation precisely.

The purpose of this utility model be achieved in that it comprise rifle body, pressurized gas inlet pipe, by the air communicating pipe between air valve, acceleration tube and the each several part of trigger control, it is characterized in that: between the top of the tail end of acceleration tube and transmitting tube, magazine is set, in magazine, fine metal mesh is set along cross section.

Above-mentioned magazine is made of magazine loam cake that is threaded and magazine bottom, and fine metal mesh clamps and is fixed between the two.

The utility model has the advantages that: simple in structure, sample is attached directly on the interior fine metal mesh of magazine, and application of sample is convenient, and inoculation is accurate, and effect obviously is better than prior art, and is cheap.

Description of drawings

Fig. 1 is the utility model structural representation.

Fig. 2 is the local enlarged diagram of magazine.

Embodiment

See Fig. 1,2, concrete structure of the present utility model is as follows: the pikestaff lower end at pistol type rifle body 9 is provided with intake interface 6, in rifle body 9, air communicating pipe 7 is set by gas flow direction, air valve 5 is set on air communicating pipe 7, and the trigger 8 (air valve 5, trigger 8 are prior art) of switch air valve 5 is set.Air communicating pipe 7 connects reducing acceleration tubes 4, at the transmitting terminal of rifle body 9 transmitting tube 1 is set, and improved technical essential is between the top of the tail end of acceleration tube 4 and transmitting tube 1 magazine 3 to be set, and magazine 3 is interior to be provided with fine metal mesh 2 along cross section, and grid is the 20-80 order.Magazine 3 is made of magazine loam cake 10 and the magazine bottom 12 that the screw thread 11 that links closely connects, and fine metal mesh 2 clamps and is fixed between the two, can change.

Using method: with leaf of Broadbean inoculation Disease-causing gene is example, at first be preparing carriers inoculum suspension with the tungsten powder, the air pump pipeline is linked on the particle gun gas connection, start air pump, it is standby when pressure reaches 0.4-0.6Mpa, draw inoculum suspension 0.5-2 μ L with microsyringe, the syringe needle of microsyringe inserted from transmitting tube 1, run into fine metal mesh 2 after, promote piston, inoculum is coated onto on the wire netting, with vanes fixed on anchor, cocking 8, gas is through quickening to enter magazine, vertically pass fine metal mesh 2 in the storehouse, drive by wicking action and be attached to dna vector particle between the wire netting grid, and by transmitting tube 1 ejaculation, finish once inoculation, extract particle gun out, open the vanes fixed frame, prepare inoculation next time.

Claims

1. simple type gas gene gun, it comprises rifle body, pressurized gas inlet pipe, by the air communicating pipe between air valve, acceleration tube and the each several part of trigger control, it is characterized in that: between the top of the tail end of acceleration tube and transmitting tube, magazine is set, in magazine, fine metal mesh is set along cross section.

2. simple type gas gene gun according to claim 1 is characterized in that: magazine is made of magazine loam cake that is threaded and magazine bottom, and fine metal mesh clamps and is fixed between the two.

3. simple type gas gene gun according to claim 1 is characterized in that: the grid of fine metal mesh is the 20-80 order.