CN201436599U - Objective table used for laser scanning confocal microscope - Google Patents
Objective table used for laser scanning confocal microscope Download PDFInfo
- Publication number
- CN201436599U CN201436599U CN200920076803XU CN200920076803U CN201436599U CN 201436599 U CN201436599 U CN 201436599U CN 200920076803X U CN200920076803X U CN 200920076803XU CN 200920076803 U CN200920076803 U CN 200920076803U CN 201436599 U CN201436599 U CN 201436599U
- Authority
- CN
- China
- Prior art keywords
- laser scanning
- sample
- objective table
- scanning confocal
- scope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Microscoopes, Condenser (AREA)
Abstract
The utility model discloses an objective table used for laser scanning confocal microscope comprises a supporting bushing, a turning screw and a plurality of adjusting bolts; one end of the turning screw is positioned in the supporting bushing, while is in threaded connection with the supporting bushing; the plurality of adjusting bolts are distributed on the supporting bushing wall; while are in threaded connection with the supporting bushing. The objective table used for laser scanning confocal microscope of the utility model can be used for mounting samples of different size specifications, provides convenience for mounting sample and regulating the sample position in the observation process.
Description
Technical field
The utility model relates to a kind of laser scanning confocal micro-scope (Laser ScanningConfocal Microscope, utility appliance LSCM), particularly a kind of objective table that is used for laser scanning confocal micro-scope.
Background technology
The confocal high temperature imager of laser scanning is the advanced precision equipment that integrates elevated temperature heat imaging heating system, high temperature stretching, extension measurement imaging heating system, laser scanning confocal micro-scope simultaneously.The confocal high temperature imager of laser scanning mainly comprises metallographic heating furnace, laser scanning confocal micro-scope and parts such as observation imaging system, worktable, temperature control system and microsystem.
Fig. 1 is the synoptic diagram of the principle of work of the confocal high temperature imager of laser scanning, as shown in the figure, wherein metallographic heating furnace 200 adopts infrared lamp 302 to focus on heating, and measures temperature by thermopair 301, shaft is oval gold-plated minute surface hermetically-sealed construction, heating-up temperature scope: 50～1600 ℃; Maximum heating speed: 300 ℃/s; Temperature fluctuation is about ± 1 ℃ during insulation, and measuring error is in 0.1%, by temperature control system 302 control and real time monitorings.The laser scanning confocal micro-scope 100 of laser scanning confocal micro-scope and observation imaging system adopts accurate confocal spatial filtering, form the unique design of image conjugation, laser pin hole on focal plane of lens forms pointolite to being arranged on tested sample (abbreviation sample) scanning in the crucible 201 on the worktable 202, optical tomographic image can be obtained after the scanning, the layer scanning and the fluorescence intensity measurement of different depth can be made with no damage to sample.The optical section of different focal planes can obtain the 3-D solid structure of sample behind three-dimensional reconstruction, and is recorded in the video file by microsystem.
Adopt advanced LSCM can in-situ observation high temperature under the tissue of material change, can analyze intuitively same sample from low temperature to high temperature up to fusing and the continuous tissue situation of change in follow-up process of setting, can reach the purpose of in-situ observation.But in actual use, also have some shortcomings:
(1) effective information is easily lost.In being observed the local microcosmos area of sample, find often such as typical organization's feature of a lot of ferrous materials, left this zone and just may can not get Useful Information and result.When adopting the confocal high temperature imager of laser scanning to carry out actual experiment; the valuable zone that we observe under simple microscope; often transferring to laser scanning confocal micro-scope following time; because two kinds of microscopical image-forming principle differences; picture contrast, contrast and brightness are also fully different; through regular meeting cause those effective microcosmos areas or feature phase, feature organization () " losing " for example: precipitated phase, second phase, bainite, martensite, pearlite, snotter etc., thus can't capture those valuable typical microstructures (phase).This problem often causes the rather expensive test of single test expense to be come back after a vain attempt, and has wasted human and material resources and financial resources.
(2) specimen preparation strictness.The sample that is used for LSCM observation is placed on little crucible and tests, and specimen size is less and strict dimensional requirement is arranged, and makes specimen preparation cumbersome.But test sometimes only needs the sample of room temperature observation, does not need sample so strict, has only needed general size just, and equipment can't carry out the room temperature observation and the process record of standard to the sample of stock size at present.
The utility model content
For addressing the above problem, the utility model provides a kind of objective table that is used for laser scanning confocal micro-scope, can place the sample of various different sizes and specification, makes things convenient for the observation of laser scanning confocal micro-scope to various samples.
For achieving the above object, the objective table that is used for laser scanning confocal micro-scope of the present utility model, comprise supporting sleeve, turn screw rod and a plurality of adjusting bolt, one end of described turn screw rod is positioned at described supporting sleeve, and be threaded with described supporting sleeve, described a plurality of adjusting bolt is distributed on the tube wall of described supporting sleeve, and is threaded with described supporting sleeve.
Described a plurality of adjusting bolt evenly distributes.
The quantity of described adjusting bolt is 4.
The objective table that is used for laser scanning confocal micro-scope of the present utility model has been expanded the function of the confocal high temperature imager of laser scanning of prior art, can be convenient to the observation in advance of sample before test or singly be used for room temperature observe.
The objective table that is used for laser scanning confocal micro-scope of the present utility model can be installed the sample of all size specification, has made things convenient for the installation of sample, and has made things convenient in observation process the adjusting to sample position.
Description of drawings
Fig. 1 is the synoptic diagram of the principle of work of the confocal high temperature imager of laser scanning;
Fig. 2 is the structural representation that is used for the objective table of laser scanning confocal micro-scope of the present utility model;
Fig. 3 is for being used for the vertical view of the objective table of laser scanning confocal micro-scope among Fig. 2;
Fig. 4 is installed on the synoptic diagram of the confocal high temperature imager of laser scanning for the objective table that is used for laser scanning confocal micro-scope of the present utility model;
Fig. 5 is fixed on the synoptic diagram that is used for the objective table of laser scanning confocal micro-scope of the present utility model for irregular sample.
Embodiment
Fig. 2 is the structural representation that is used for the objective table of laser scanning confocal micro-scope of the present utility model, and Fig. 3 is for being used for the vertical view of the objective table of laser scanning confocal micro-scope among Fig. 2.As shown in the figure, the objective table that is used for laser scanning confocal micro-scope of the present utility model comprises supporting sleeve 1, turn screw rod 2 and a plurality of adjusting bolt 3, one end of described turn screw rod 2 is positioned at described supporting sleeve 1, and be threaded with described supporting sleeve 1, described a plurality of adjusting bolt 3 is evenly distributed on the tube wall of described supporting sleeve 1, and is threaded with described supporting sleeve 1.
The end of turn screw rod 2 and a plurality of adjusting bolt 3 constitute the put area of sample 10 together.Sample 10 places the end of described turn screw rod 2, can regulate the upper-lower position of sample 10 by rotating described turn screw rod 2, by a plurality of adjusting bolts 3 on the supporting sleeve 1 fit adjustment together, can regulate the horizontal level of sample on the end plane of described turn screw rod 2.So just can realize the three-dimensional regulation of the position of sample 10, make things convenient for the observation to sample 10 of laser scanning confocal micro-scope.After regulating the position, screw described a plurality of adjusting bolt 3, can clamp sample 10.
On supporting sleeve 1, be provided for the threaded hole that is connected with worktable 202, the convenient objective table that is used for laser scanning confocal micro-scope of the present utility model fixing.
The quantity of described adjusting bolt 3 can determine that preferably, the quantity of described adjusting bolt 3 is 4 according to the shape of sample, and four are symmetrical arranged the angular direction.
The objective table that is used for laser scanning confocal micro-scope of the present utility model in use, can directly sample 10 be placed on the end of turn screw rod 2, the tested plane of sample 10 upwards, after rotation turn screw rod 2 is adjusted to correct position, adjusting adjusting bolt 3 then fixes sample behind correct position, simultaneously the objective table worktable 202 that is used for laser scanning confocal micro-scope of the present utility model is fixed, just can observe, the objective table that is used for laser scanning confocal micro-scope of the present utility model as shown in Figure 4 is installed on the synoptic diagram of the confocal high temperature imager of laser scanning.The objective table that is used for laser scanning confocal micro-scope of the present utility model can be arranged on the confocal high temperature imager of existing laser scanning next door, shared same worktable 202.Before the observation, with confocal microscope move on to objective table of the present invention directly over position fixing.
As needing the local position all around of adjusting sample 10, can realize in the observation process by fine setting adjusting bolt 3; Manual adjustments turn screw rod 2 can be realized the fine adjustment function up and down of sample 10.
Fig. 5 is fixed on the synoptic diagram that is used for the objective table of laser scanning confocal micro-scope of the present utility model for irregular sample, as shown in the figure, the sample 11 not parallel for upper and lower surface or the bottom is irregular (tested surface is a surface polishing), during installation, on Working table, put earlier a lens paper, tested surface upper and lower surface is not parallel or the irregular sample 11 in bottom is placed on the lens paper, and the softening plasticine 4 of the sticking lastblock in bottom is used for bonding with turn screw rod 2 ends; Objective table back-off of the present utility model, surround sample 11 then, make the upper surface of supporting sleeve 1 and sample 11 tested surface in one plane; Rotating turn screw rod 2 then makes sample fix; Then turn screw rod 2 is screwed on gently, make its end gently by sample 11 lower surfaces, softening plasticine 4 can play the effect that prevents the random tilting of sample; At last the objective table integral body that installs sample 11 is turned over, be installed in again on the worktable 202 and observe.
Objective table of the present utility model is observed in advance to the room temperature of sample microstructure before being used in test, accomplishes a understanding roughly in advance, particularly can locate in advance typical microcosmos area, carries out the high temperature heating and the observation of sample subsequently.Like this, owing to be the microstructure pattern of observing at same microscopically, on the color and luster of image, brightness and contrast, all do not have to change, specific aim has been arranged so we just can make things convenient for and like clockwork the microstructure in the sample later stage heating process be changed observation.On the other hand, utilize the higher CONFOCAL SCANNING LASER MICROSCOPE of image quality, can carry out the record of room temperature observation and microstructure to the sample of other various sizes, can Taking Pictures recording, can also carry out videograph to the observation process, realize the continuous recording of observation process and obtaining continuously of micro-image.
Claims (3)
1. objective table that is used for laser scanning confocal micro-scope, it is characterized in that, comprise supporting sleeve, turn screw rod and a plurality of adjusting bolt, one end of described turn screw rod is positioned at described supporting sleeve, and be threaded with described supporting sleeve, described a plurality of adjusting bolt is distributed on the tube wall of described supporting sleeve, and is threaded with described supporting sleeve.
2. the objective table that is used for laser scanning confocal micro-scope as claimed in claim 1 is characterized in that, described a plurality of adjusting bolts evenly distribute.
3. the objective table that is used for laser scanning confocal micro-scope as claimed in claim 1 is characterized in that, the quantity of described adjusting bolt is 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200920076803XU CN201436599U (en) | 2009-06-22 | 2009-06-22 | Objective table used for laser scanning confocal microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200920076803XU CN201436599U (en) | 2009-06-22 | 2009-06-22 | Objective table used for laser scanning confocal microscope |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201436599U true CN201436599U (en) | 2010-04-07 |
Family
ID=42373049
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200920076803XU Expired - Fee Related CN201436599U (en) | 2009-06-22 | 2009-06-22 | Objective table used for laser scanning confocal microscope |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201436599U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103048300A (en) * | 2012-12-17 | 2013-04-17 | 江苏大学 | Confocal laser scanning microscope |
-
2009
- 2009-06-22 CN CN200920076803XU patent/CN201436599U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103048300A (en) * | 2012-12-17 | 2013-04-17 | 江苏大学 | Confocal laser scanning microscope |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7630065B2 (en) | Low-light specimen image pickup unit and low-light specimen image pickup apparatus | |
| CN201266175Y (en) | Three-dimensional displacement micro observation device | |
| CN105547182A (en) | Spinneret plate detection equipment and method | |
| El-Badry | Micromanipulators and micromanipulation | |
| CN107357031A (en) | A kind of mirror image microscopic imaging device and automated micro-manipulation pin pose calibrating system and method | |
| CN108375584A (en) | Infrared optical material microdefect detection device and far infrared microlens | |
| CN201436599U (en) | Objective table used for laser scanning confocal microscope | |
| Trache et al. | Integrated microscopy for real-time imaging of mechanotransduction studies in live cells | |
| TW554177B (en) | Microscope unit | |
| CN215179723U (en) | Microscopic observation device for pathogenic biology | |
| CN104931388A (en) | Rheological in-situ online test system integrating scattering and microscopy | |
| CN208421395U (en) | A kind of Optical devices of multi-angle observation | |
| US7839496B2 (en) | Confocal microscopy pad sample holder and method of hand using the same | |
| DE102017111287B4 (en) | Light microscope with temperature-controlled condenser | |
| CN201225924Y (en) | Light path system for measuring and observing color | |
| WO2010033100A1 (en) | Probe alignment tool for the scanning probe microscope | |
| CN108080783A (en) | A kind of focusing mechanism and laser-processing system | |
| CN209342508U (en) | A kind of material strain original position EBSD observation test fixture | |
| CN208421405U (en) | A kind of heavy caliber parallel light tube focal plane high-precision is split as autocollimatic marking apparatus | |
| US10365464B1 (en) | Extending optical microscopes to provide selective plane illumination microscopy | |
| CN206804987U (en) | A kind of Embryo tissue teaching equipment | |
| CN207516632U (en) | Imaging auxiliary device with microballoon | |
| JP3137634U (en) | Macro micro navigation system | |
| CN217845101U (en) | Three-dimensional detector | |
| CN2893729Y (en) | Level microscope system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100407 Termination date: 20160622 |