CN1948230A - Chromium-rich liquid and its use in production of chromium (Cr) -rich liquid3+) Application of pumpkin - Google Patents
Chromium-rich liquid and its use in production of chromium (Cr) -rich liquid3+) Application of pumpkin Download PDFInfo
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- 239000011651 chromium Substances 0.000 title claims abstract description 205
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 title claims abstract description 174
- 229910052804 chromium Inorganic materials 0.000 title claims abstract description 129
- 235000000832 Ayote Nutrition 0.000 title claims abstract description 87
- 235000009854 Cucurbita moschata Nutrition 0.000 title claims abstract description 87
- 240000001980 Cucurbita pepo Species 0.000 title claims abstract description 87
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 title claims abstract description 87
- 235000015136 pumpkin Nutrition 0.000 title claims abstract description 87
- 239000007788 liquid Substances 0.000 title abstract description 10
- 238000004519 manufacturing process Methods 0.000 title abstract description 6
- 241000196324 Embryophyta Species 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 7
- 235000013399 edible fruits Nutrition 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 15
- 239000007921 spray Substances 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 12
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 230000003442 weekly effect Effects 0.000 claims description 5
- 230000012010 growth Effects 0.000 claims description 3
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- 229920001213 Polysorbate 20 Polymers 0.000 abstract 1
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- 230000001502 supplementing effect Effects 0.000 abstract 1
- 235000012721 chromium Nutrition 0.000 description 104
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Abstract
The invention discloses a method for producing chromium (Cr) -rich material3+) Chromium-rich liquid of pumpkin and application of chromium-rich liquid to production of chromium (Cr) -rich liquid3+) The application of pumpkin, and the prepared chromium-rich liquid consists of the following raw materials: cr (chromium) component2O3：500mg/kg～3000mg/kg，KH2 (PO4)：5g/kg～10g/kg，K2MnO4: 1 g/kg-5 g/kg, tween 20: 2ml/L and the balance of water. Can be used for producing chromium (Cr) -rich material3+) Application of fructus Cucurbitae Moschatae is provided. Under the optimized cultivation condition, the chromium-rich pumpkin variety Yongan No. 2 is taken as a seed, the planting is carried out according to a normal cultivation method, the chromium-rich liquid is sprayed on the leaf surface of the pumpkin in the evening of sunny days when the blossom is started, the spraying amount is 50 ml-100 ml/plant/time, and then the chromium-rich pumpkin is sprayed once a week for 3 times continuously; then the pumpkin is managed according to normal field until the pumpkin grows mature, the chromium content in the pumpkin pulp is improved by more than 10 times than that of the contrast, and meanwhile, the proportion of organic chromium is increased. Can be used as natural biological raw material for supplementing chromium, and can be widely applied to fresh vegetables, various health foods, beverages and industrial raw materials for extracting chromium compounds.
Description
Technical field
The invention belongs to the plant husbandry technical field, relate to a kind of blade face spray, particularly a kind of be used for producing be rich in chromium (Cr
3+) pumpkin rich chrome liquor and should the richness chrome liquor be used for producing and be rich in chromium (Cr
3+) application of pumpkin.
Technical background
Chromium (Cr) is the essential a kind of trace element of animal and human's body, extensively be present in soil, the water, number of chemical valence state: Cr (VI), Cr (II), Cr (III), Cr (IV) etc. are arranged, and wherein Cr (VI) and Cr (III) valence state are relatively stable, account for total chromium ratio height.Studies show that, Cr (VI) is harmful carcinogenic substance, and Cr (III) is the chemical substance to the human body beneficial, main biological function show with human body in protein, nucleic acid and in each, the part combination of small molecular weight, in carbohydrate metabolism and metabolism of fat, with Regular Insulin synergy or as the toughener of Regular Insulin, thereby bring into play its physiological function; In nucleic acid metabolism, participate in the regulation and control with genetic expression kept of nucleic acid molecule thigh structural integrity.Human body lacks chromium, can cause the metabolic disorder of body carbohydrate and fats, and causes the appearance of relative disease (diabetes).Anderson RA (1985) studies show that, human body is 10%～25% to the specific absorption of organic chromium, and the specific absorption of unconverted inorganic chromium is had only 0.05%.Because chromium be a large amount of drainages of chromium in a large amount of losses in non-recycling element, the food precision work process and the precision work food promotion body in human body, human body lacks the chromium ubiquity.
The representative research of mending chromium and mending the chromium product for human body is (1999) such as Feng Weiyue of unit such as Institute of High Energy Physcis, Academia Sinica etc. (1998) and Ding Wenjuns, they have successively studied the chemical form and the combined of chromium in the yeast, filtered out Rich chromium yeast, can make the transformation efficiency of chromium reach 84%, yet, influence the human body chrome uptake because the yeast hard shell is nondigestible.Therefore, the chromium in China's human body source at present still mainly by plant-sourced food mend into.
In China, the intravital chromium of plant is mainly derived from soil.The chromium content of China's soil is 30～110mg/kg, average out to 72mg/kg, and except only a few rock decay district and industrial pollution district, chromium content is lower in most agricultural land soils; And the root system of plant is very limited for chrome uptake, because the relation of transporter in the plant materials, absorbs the back and is transported to overground part by subterranean root and preserves the chromium of product organ very little especially.The chromium content of general plant edible food organ only be ppm (as, chromium content in the flour is 0.23mg/kg, Semen Maydis powder is 0.11mg/kg, rice is 0.43mg/kg, pumpkin is 0.07～2.04mg/kg), and the rich chromium pumpkin variety that the applicant cultivates can improve 3～7 times with the chromium content in the pumpkin.Yet the organic chromium in the plant materials is by absorption by human body utilization ratio only 10～25%, and normal adult day by day chromium be 50 μ g～100 μ g.Therefore, the plant-sourced food of low chromium content can not satisfy the needs of human body to chromium.
From plant, extract beneficial compound, and then to make its commercialization, medicineization be the frontier of modern agricultural development, if chromium cpd can extract in the plant, separation, purifying, further medicineization, will for the mankind fundamentally effect a radical cure diabetes provide the important channel (chromium can be repaired human islet cell, improve Regular Insulin in the identification on target cell surface and enter ability and increase the effect of Regular Insulin).Pumpkin is the plant of enrichment chromium, and the characteristic of its suitable processing becomes the first-selected plant of industrial extraction chromium cpd in addition, because chromium content is on the low side in the existing pumpkin, influences carrying out of this work.
Produce rich chromium (Cr
3+) the pumpkin product no matter for the common people's daily life with healthy, still for agricultural-food enterprise provides rich chromium starting material, all seem very important and urgent.But have not yet to see the report of rich chromium pumpkin product or other rich chromium plant prods.
Summary of the invention
The objective of the invention is to, provide a kind of be used for producing be rich in chromium (Cr
3+) pumpkin rich chrome liquor and should the richness chrome liquor be used for producing and be rich in chromium (Cr
3+) application of pumpkin, the pumpkin fruit that is rich in chromium for production is opened up a new approach.
In order to realize above-mentioned task, the present invention takes following technical solution:
A kind of be used for producing be rich in chromium (Cr
3+) the rich chrome liquor of pumpkin, it is characterized in that the forming of this richness chrome liquor that makes: Cr by following raw materials according
2O
3: 500mg/kg～3000mg/kg, KH
2(PO
4): 5g/kg～10g/kg, K
2MnO
4: 1g/kg～5g/kg, polysorbas20: 2ml/L, surplus is a water.
Above-mentioned be used for producing be rich in chromium (Cr
3+) preparation method of rich chrome liquor of pumpkin, it is characterized in that, carry out according to the following steps:
Step 1: by the prescription of rich chrome liquor, earlier with KH
2(PO
4) insert in the water, stirring makes it be dissolved into potassium dihydrogen phosphate fully;
Step 2: in potassium dihydrogen phosphate, add Cr
2O
3. stirring is dissolved it fully;
Step 3: add K again
2MnO
4Add again after stirring is dissolved it fully polysorbas20 stir evenly after promptly.
Rich chrome liquor of the present invention is suitable for promptly joining promptly using.
Above-mentioned be used for producing be rich in chromium (Cr
3+) the rich chrome liquor of pumpkin can be used for producing and be rich in chromium (Cr
3+) application of pumpkin.Its application method is to be that Yongan is for No. 2 seed with rich chromium pumpkin variety, plants according to normal cultivating method, treats just to spend phase beginning, in fine day at dusk to the rich chrome liquor of pumpkin foliage spray, the sprinkling amount be the 50ml/ strain/time～the 100ml/ strain/time, spray once continuous 3 times afterwards weekly; Can obtain being rich in chromium (Cr by normal field management to pumpkin growth and maturity then
3+) pumpkin, the comparison of chromium content is according to improving more than 10 times in its pumpkin fruit.
Of the present invention be used for producing be rich in chromium (Cr
3+) the rich chrome liquor of pumpkin, optimizing under the cultivation condition, the chromium content of pumpkin obviously can increasing, the ratio of organic chromium also increases thereupon simultaneously.Can be used as human body and mend chromium natural biological raw material, be widely used in eating raw the industrial raw material that vegetables, various protective foods, beverage and chromium cpd extract.
Embodiment
1. rich chrome liquor prescription is determined
(1) the chromium source is determined
Cr can be used in the chromium source of rich chrome liquor
2O
3Or K
2CrO
4,, compare test for determining the chromium source.Step is as follows:
1. to cultivate with the little mill of farmers''s pumpkin variety be Cultivar to the pumpkin sample, fine at initial bloom stage at dusk to the rich chrome liquor of pumpkin foliage spray, the sprinkling amount be the 50ml/ strain/time～the 100ml/ strain/time, spray once continuous 3 times afterwards weekly; Promptly get the pumpkin sample by normal field management to pumpkin growth and maturity then.
2. Cr content and chemical form are measured in the sample
Step 1 is for avoiding the interference of chromium in the stainless steel instruments, and all operations does not all contact with stainless steel instruments.All glass and plastic tool are through 1: 1HNO
3With 1: 1HCl alternately soaks 72h, rinses, dries that to put in the plastics bag sealing standby well with deionized water.
Step 2 is ground to commercially available anionite-exchange resin Powdered in mortar, cross the 150mm sieve, 4h, filtration under diminished pressure then again are soaked in water, remove bubble, wash with water to clarification, soak 8h with 6 times of 2mol/LHCl, 2mol/LNaOH respectively again after anhydrating, remove disacidify, alkali lye to the resin volume, wash with water to neutrality, after repeating once again, put 60 ℃ of down oven dry, it is standby to be put in the wide-necked bottle sealing.
Total chromium (C in step 3 pumpkin
RT) measure the pumpkin sample is taken by weighing sample 5g ± 0.1mg after with plastic tool chopping, mixing in dry beaker, add high-purity HNO
3When 10mL, cap surface ware are 3mL in hot digestion on the hot plate to solution, add 5mLH
2O
2Continue to digest transparent and evaporate to dryness to solution.Add 2mL deionized water dissolving residue, solution is transferred in the 10mL volumetric flask, behind scale, directly measures Cr content with the Zeeman graphite oven atomic absorption with the deionized water constant volume, is C
RT
Total chromium (the C of soluble state in step 4 pumpkin
RTS) measure and to take by weighing above-mentioned pumpkin sample 100g ± 0.1mg in the 1000mL volumetric flask, add 0.7mol/LHCL solution 400mL, soak in the rearmounted 100 ℃ of water-baths of 72h and be incubated 12h, it is centrifugal to leave standstill the back, supernatant liquor is collected filtrate and is directly measured Cr content with the Zeeman graphite oven atomic absorption through 0.45 μ m membrane filtration.Be C
RTSContent.
Soluble state trivalent chromium (Cr in step 5 pumpkin
+ 3) measure the filtered liquid of getting an amount of above-mentioned 0.45 μ m film, make it pass through anion-exchange resin column with certain flow rate (1.0mol/min), the collection post passes liquid and measures with Zeeman sampling Graphite Furnace Atomic Absorption method.Be Cr
+ 3Content.
Total chromium (the C of soluble attitude in step 6 pumpkin
RTI) and soluble state sexavalent chrome (Cr
+ 6) calculating
Total chromium (the C of soluble attitude in the pumpkin
RTI)=total chromium (C
RTTotal chromium (the C of)-middle soluble state
RTS)
Soluble state sexavalent chrome (Cr in the pumpkin
+ 6Total chromium (the C of)=soluble state
RTS)-soluble state trivalent chromium (Cr
+ 3)
Soluble state organic chromium (Cr in step 7 pumpkin
o) measure get an amount of above-mentioned 0.45 μ m film filtered liquid in the 150mL separating funnel, use 60mL CHCl
3Divide 3 extractions, collect organic phase and use the Ar air-blowing to do, wet method digestion back is Cr with Zeeman sampling Graphite Furnace Atomic Absorption method mensuration
oContent.
Through aforesaid method, get measurement result (table 1)
The chemical form measurement result of chromium in table 1 pumpkin
| The form of chromium | Cr 2O 3Cr μ g/kg (fresh weight) | Account for Cr T/％ | K 2CrO 4Cr μ g/kg (fresh weight) | Account for Cr T/％ |
| Total chromium Total (Cr T) the total chromium (Cr of soluble state TS) trivalent chromium Cr +3Sexavalent chrome Cr +6Organic chromium (Cr O) the total chromium (Cr of soluble attitude TI) | 96.46 87.86 80.17 7.69 3.54 8.60 | 91.08 83.11 7.97 7.32 8.92 | 95.87 86.43 67.25 12.49 6.69 9.44 | 90.15 70.15 13.02 6.92 9.84 |
As seen from Table 1, Cr
2O
3Due to total Cr of soluble state and Cr (III) content height in the pumpkin fruit, and to the human body beneficial, K
2CrO
4Due in the pumpkin fruit except that Cr (III) is arranged, total Cr of soluble attitude and Cr (VI) content are higher, and Cr (VI) is harmful.Therefore, only select Cr for use
2O
3Chromium source as rich chrome liquor.
(2) chromium concn determines
By 100mg/kg～10000mg/kg totally 8 concentration level simultaneous tests, measure total cr content (concrete operations are with aforementioned) in the pumpkin fruit with the GFAAS method, the result shows, chromium content in the pumpkin fruit improves along with the increase of chromium concn in the rich chrome liquor, and when concentration 500mg/kg～3000mg/kg, the chromium content in the pulp is high and stablize, the pumpkin blade face does not show untoward reaction, when concentration during greater than 3000mg/kg, the chromium content in the pulp does not have corresponding raising, and chromium evil symptom appears in the blade face.Therefore the suitable concentration of determining chromium is 500～3000mg/kg.
(3) chromium absorbs auxiliary agent KH
2PO
4, K
2MnO
4Determine
As everyone knows, K, P, Mn are the necessary element of plant-growth, and K helps the transportation of nutritive substance in the plant materials, P is the formation element of plant life material, and Mn can promote plant-growth, by the having of above-mentioned element, it's too late concentration simultaneous test, according to the variation of chromium content in the pulp, determine KH
2PO
4Suitable concentration is (5～10) g/kg, K
2MnO
4Concentration is (1～5) g/kg.
(4) selection of polysorbas20
For improving the tackiness of rich chrome liquor on the pumpkin blade face, prolong in the residence time on blade face, increase the chance of Foliage Absorption, add the polysorbas20 of 2ml/L.
According to above-mentioned research, form the prescription of rich chrome liquor:
Cr
2O
3 (500～3000)mg/kg
KH
2(PO
4) (5～10)g/kg
K
2MnO
4 (1～5)g/kg
Polysorbas20 2ml/L
All the other water
2, rich chrome liquor preparation
(1) by the prescription of rich chrome liquor, elder generation is with KH
2(PO
4) insert in the water, stirring makes it be dissolved into potassium dihydrogen phosphate fully;
(2) in potassium dihydrogen phosphate, add Cr
2O
3, stirring is dissolved it fully;
(3) add K again
2MnO
4Stirring is dissolved it fully, add at last polysorbas20 stir evenly after promptly;
(4) rich chrome liquor is promptly joined promptly and is used.
3, the application of rich chrome liquor
(1) carries out pumpkin planting according to normal cultivating method
Studied chromium in pumpkin metabolic process and the basis of the environmental factors influence that pumpkin absorbed chromium on the application method of the rich chrome liquor of research.With K
2CrO
4Be carrier, use
51Cr isotopic labeling, intensity of radioactivity are 200 μ Ci/ strains, add scintillation solution in the digestion sample, measure with liquid glimmer instrument
51The radioactivity of Cr is carried out mark to the pumpkin that is in different growing stage (seedling phase, take out climing phase, florescence, fruiting period), adopts blade is smeared, root applies method research Cr at the intravital distribution of pumpkin and the pumpkin absorption dynamics to Cr.After successively marker is measured.Tentatively verify: seedling stage the root mark
51Cr, absorption and transport be after 10 days, in the nearer blade of root
51The Cr semi-invariant is the highest, along with the increase of root distance, in the blade
51Cr content successively decreases successively; Florescence root mark
51Cr contains in blade and fruit
51Cr is in the functional leaf of wherein climing middle part
51Cr content is higher than other blade; Blade is smeared mark
51Cr is in the near blade and fruit of mark position
51Cr content is higher relatively; Smear mark and apply marking method with root and compare, each organ Cr content of pumpkin is generally on the low side; Dynamics research is the result show,
51Cr
6+Can absorption and transport in 24 hours in pumpkin to other organ of overground part.Right in the seedling phase pumpkin body
51The absorption of Cr distributes to pull climing phase, initial bloom stage and fruiting period similar.Tentatively think thus: root is the major organs that absorbs Cr, Cr is to the flower of plant, fruit, and growing end transportation from root at the intravital transporting direction of pumpkin, seldom reverse transportation, Cr distributes in the pumpkin body and is followed successively by root Cr content＞stem, leaf＞flower＞fruit.In addition, from the distribution of plant overground part, Cr content and organ photosynthesis power have certain relation.
(2) use-pattern determines
According to above-mentioned result of study, greater than leaf portion, but amount is transported to overground part to pumpkin because the chromium that root absorbs only has seldom to the chrome uptake root, and transportation is accumulated in the pumpkin fruit chromium then still less; The chromium that is manured into soil only has part to be absorbed, and leaves over part and can produce pollution to soil.Therefore, adopt the mode～leaf portion that applies to spray on the ground.
(3) use age determines
According to the transporting direction of chromium in pumpkin and the time that transforms in vivo, the simultaneous test of handling by the phase of executing 6 of seedling stage～fructescences according to chromium content in the fruit, determines that the best is that gradation applies better effects if in 2 weeks behind the initial bloom stage period of applying.
(4) duration of service determines
On spraying time, spray at fine morning, noon, afternoon, dusk and different weathers such as cloudy day, light rain sky, different time respectively, measure chromium content and the plant reaction observed in the pumpkin fruit, spray and very easily cause poisoning fine noon, to spray assimilation effect the poorest because of, rainy day, and fine effect is best at dusk.
(5) usage quantity determines
By sowing amount (10,50,100,150,200ml/ strain/time), apply number of times and the multilevel comparison test of multiplefactor of pitch time (3,7,14,21,28,35,40 days), with initial bloom stage for just executing the phase, per 7 days once, continuous 3 times, the treatment effect of each 50～100ml/ strain is best.
According to above-mentioned research, it is as follows to form rich chrome liquor application method:
Treat just to spend phase beginning, in fine day at dusk to the rich chrome liquor of pumpkin foliage spray.The sprinkling amount be 50ml～100ml/ strain/time.Weekly afterwards, continuous 3 times.
Under the normal management of field, the comparison of chromium content is according to improving more than 10 times in the pumpkin fruit of physiological maturity (spending back about 40 days).
2004, the applicant used the present invention in Shaanxi Yang Ling area and verifies.With 4 pumpkin materials such as J121-12-55 is Cultivar, the chromium content 57.9mg/kg in the cultivating soil, the chromium content 0.009mg/kg in the irrigation water.The fine dusk of Yu Chuhua phase beginning is to the rich chrome liquor of pumpkin foliage spray.The sprinkling amount be 50ml～100ml/ strain/time.Weekly afterwards, continuous 3 times.Under the normal management of field, measure the chromium content (measuring method is with aforementioned) in physiological maturity (spending back about the 40 days) pumpkin fruit, the results are shown in Table 2 (referring to annexes 1).
The rich chrome liquor enrichment of table 2 chromium effect relatively
| Experimental cultivar | Do not spray the chromium content mg/kg of rich chrome liquor | Spray the chromium content mg/kg of rich chrome liquor | The multiple that improves |
| J121-12-58 | 0.034 | 0.450 | 13.23 |
| Day-P2-55 | 0.039 | 0.410 | 10.51 |
| No. 2, Yongan | 0.0125 | 0.480 | 38.40 |
| 035 * forever 2 | 0.009 | 0.130 | 14.4 |
As seen from Table 2, use this richness chrome liquor and use according to standard method, can make the content of chromium in the pumpkin fruit improve more than 10 times, the highlyest improve 38.4 times, reach the purpose that significantly improves chromium content in the pumpkin, owing to do not detect sexavalent chrome in the sample, almost be trivalent chromium entirely in the interpret sample, chromium content in the soil does not have obvious variation (increasing by 1.69%, less than 5% error at measurment) simultaneously, illustrates that rich chrome liquor of the present invention is rich in chromium (Cr in production
3+) use of pumpkin can not pollute edatope.Proved the production that should the richness chrome liquor can be used for rich chromium pumpkin.
Claims (4)
1. one kind is used for producing and is rich in chromium (Cr
3+) the rich chrome liquor of pumpkin, it is characterized in that the forming of this richness chrome liquor that makes: Cr by following raw materials according
2O
3: 500mg/kg～3000mg/kg, KH
2(PO
4): 5g/kg～10g/kg, K
2MnO
4: 1g/kg～5g/kg, polysorbas20: 2ml/L, surplus is a water.
Claim 1 described be used for producing be rich in chromium (Cr
3+) preparation method of rich chrome liquor of pumpkin, it is characterized in that, carry out according to the following steps:
Step 1: by the prescription of rich chrome liquor, earlier with KH
2(PO
4) insert in the water, stirring makes it be dissolved into potassium dihydrogen phosphate fully;
Step 2: in potassium dihydrogen phosphate, add Cr
2O
3, stirring is dissolved it fully;
Step 3: add K again
2MnO
4Add again after stirring is dissolved it fully polysorbas20 stir evenly after promptly.
Claim 1 described be used for producing be rich in chromium (Cr
3+) the rich chrome liquor of pumpkin is used for producing and is rich in chromium (Cr
3+) application of pumpkin.
4. application as claimed in claim 3, it is characterized in that, with rich chromium pumpkin variety is that Yongan is for No. 2 seed, plant according to normal cultivating method, treat just to spend phase beginning, in fine day at dusk to the rich chrome liquor of pumpkin foliage spray, the sprinkling amount be the 50ml/ strain/time～the 100ml/ strain/time, spray once continuous 3 times afterwards weekly; Can obtain being rich in chromium (Cr by normal field management to pumpkin growth and maturity then
3+) pumpkin, the comparison of chromium content is according to improving more than 10 times in its pumpkin fruit.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101048450A CN100418931C (en) | 2006-11-02 | 2006-11-02 | Chromium-rich liquid and its use in production of chromium (Cr) -rich liquid3+) Application of pumpkin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101048450A CN100418931C (en) | 2006-11-02 | 2006-11-02 | Chromium-rich liquid and its use in production of chromium (Cr) -rich liquid3+) Application of pumpkin |
Publications (2)
| Publication Number | Publication Date |
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| CN1948230A true CN1948230A (en) | 2007-04-18 |
| CN100418931C CN100418931C (en) | 2008-09-17 |
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| CN (1) | CN100418931C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325840A (en) * | 2015-10-26 | 2016-02-17 | 李明山 | Preparation method of chromizing cellulose |
| CN106034711A (en) * | 2016-07-01 | 2016-10-26 | 广西***琼达农业科技有限公司 | High-yield cultivation method for organic chromium-enriched flemingia philippinensis |
| CN106613157A (en) * | 2016-11-22 | 2017-05-10 | 蚌埠市涂山绿园蔬菜科研专业合作社 | Seedling strengthening cultivation method of chromium-rich pumpkins |
| CN108371027A (en) * | 2016-10-26 | 2018-08-07 | 梁春林 | A kind of richness Organic Chromium yam bean high yield cultivating method |
| CN108713681A (en) * | 2018-05-23 | 2018-10-30 | 广东日可威富硒食品有限公司 | A kind of weight-reducing disappears the health-preserving noodle of fat |
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| CN108522145A (en) * | 2018-05-11 | 2018-09-14 | 福建省农业科学院农业质量标准与检测技术研究所 | A kind of white fungus culture medium and cultivating white fungus method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1059422C (en) * | 1997-03-28 | 2000-12-13 | 苏双 | Fertilizer synergist and all-element composite fertilizer prepared by same |
| CN1062255C (en) * | 1997-06-19 | 2001-02-21 | 金全 | Compound fertilizer and producing method thereof |
| CN1061020C (en) * | 1997-11-24 | 2001-01-24 | 刘文齐 | Mixed fertilizer and its production |
| CN1154412C (en) * | 1999-07-20 | 2004-06-23 | 王将克 | Soilless cultivation method of seed sprout and seed sprout vegetable containing trace element |
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2006
- 2006-11-02 CN CNB2006101048450A patent/CN100418931C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105325840A (en) * | 2015-10-26 | 2016-02-17 | 李明山 | Preparation method of chromizing cellulose |
| CN106034711A (en) * | 2016-07-01 | 2016-10-26 | 广西***琼达农业科技有限公司 | High-yield cultivation method for organic chromium-enriched flemingia philippinensis |
| CN108371027A (en) * | 2016-10-26 | 2018-08-07 | 梁春林 | A kind of richness Organic Chromium yam bean high yield cultivating method |
| CN106613157A (en) * | 2016-11-22 | 2017-05-10 | 蚌埠市涂山绿园蔬菜科研专业合作社 | Seedling strengthening cultivation method of chromium-rich pumpkins |
| CN108713681A (en) * | 2018-05-23 | 2018-10-30 | 广东日可威富硒食品有限公司 | A kind of weight-reducing disappears the health-preserving noodle of fat |
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| Publication number | Publication date |
|---|---|
| CN100418931C (en) | 2008-09-17 |
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