CN1880445A - Tinder fungus and process for deep liquid fermentation preparation of tinder fungus - Google Patents
Tinder fungus and process for deep liquid fermentation preparation of tinder fungus Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000000855 fermentation Methods 0.000 title claims description 27
- 230000004151 fermentation Effects 0.000 title claims description 27
- 238000002360 preparation method Methods 0.000 title claims description 14
- 241000233866 Fungi Species 0.000 title description 4
- 230000001580 bacterial effect Effects 0.000 claims abstract description 20
- 238000011218 seed culture Methods 0.000 claims abstract description 17
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000007787 solid Substances 0.000 claims abstract description 7
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 10
- 108010080698 Peptones Proteins 0.000 claims description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 10
- 235000019319 peptone Nutrition 0.000 claims description 10
- 239000002054 inoculum Substances 0.000 claims description 8
- 239000004615 ingredient Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
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- 241000222341 Polyporaceae Species 0.000 claims description 6
- 241000222383 Polyporales Species 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
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- 229940041514 candida albicans extract Drugs 0.000 claims description 4
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- 241001149422 Ganoderma applanatum Species 0.000 claims description 3
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- 238000000746 purification Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 230000001954 sterilising effect Effects 0.000 description 10
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- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
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- 239000008223 sterile water Substances 0.000 description 1
- 201000000498 stomach carcinoma Diseases 0.000 description 1
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- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Natural products CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 1
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- 235000007586 terpenes Nutrition 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a wood-foot layer hole-bacteria and thalline preparing method of wood-foot layer hole-bacteria through liquid deep layer ferment, which is characterized by the following: the wood-foot layer hole-bacteria is reserved in the China microbe bacterial preservation and management committee common microbe center, whose reserving number is CGMCC No.1658 in the Basidiomycetes Polyporales Polyporaceae Fomes. The invention is cultivated by artificial through solid incidence culture, concentrating table seed culture, fermenter culture, which produces dry weight of mycelium in the ferment liquid at 10-15g/L.
Description
Technical field
The invention belongs to the microbial engineering field, relate to the method that a kind of Fomes fomentarius and liquid submerged fermentation prepare the Fomes fomentarius thalline.
Background technology
Because the scarcity of wild resource and the deterioration of growing environment, many famous and precious medicinal fungis are endangered, how to save these valuable medicine resources, satisfy the drug development needs and seem particularly important, utilize microbial technique to separate active bacterial strain, adopt modern fermentation technique to carry out large-scale medicinal material and prepare necessary.
Fomes fomentarius (Fomes fomentarius) is a kind of rare Chinese herbal medicine, and the use among the people of China the Northeast is comparatively general, has a long history.Its sporophore includes abundant terpene, Polyphenols, alkaloid and polysaccharide, pharmaceutical use is high aspect the treatment human malignant tumor, be mainly used in the tumour of Digestive tract such as treatment esophagus cancer, cancer of the stomach, its decocting decoction is in treatment children's having indigestion, and the long-pending stagnation resolvation aspect that disappears also has unusual effect.
The modern fermentation technique that the eighties of last century middle and later periods grows up has been successfully applied to the mass preparation of multiple medicinal fungi, and is wherein ripe with liquid fermentation technology.This still belongs to the first at home with the preparation that liquid fermentation technology is applied to Fomes fomentarius hyphostroma, and result of study shows that this scheme is feasible.
Summary of the invention
The purpose of this invention is to provide the method that a kind of Fomes fomentarius and liquid submerged fermentation prepare the Fomes fomentarius thalline.
Fomes fomentarius (Fomes fomentarius) is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is: CGMCC No.1658, belong to Basidiomycetes (Basidiomycetes), Aphyllophorales (Polyporales), polyporaceae (Polyporaceae), shelf fungus belongs to (Fomes).
Liquid submerged fermentation prepares the method for Fomes fomentarius thalline: the Fomes fomentarius bacterial strain that separation and purification obtains from the wild wooden hoof entity, be the bacterial classification that sets out with this bacterial strain, through solid slant culture, table concentration seed culture, fermentor cultivation, prepare Fomes fomentarius hyphostroma successively.
The internal transcribed spacer district ribosomal dna sequence of described bacterial strain is as follows:
CCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGGTCAGAGTTCATAAAA
GCTGTCTCTGACGAGACCATTAGAAGCTCTCCAAACGCTTCACGGTCGCGGCGTAGACATTATCACAC
CGACAGCCGATCCGCAAGGAACCAAGCTAATGCATTTGAGAGGAGCTGACTCAAACGAGGGCCAGCAA
AAGCCTCCAATAAGCCAACCCTACAAACCCGCAAAGGTTTATAGGTTGAGAATTTCATGACACTCAAA
CAGGCATGCTCCTCGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGATTCGATGATTCACTGAATTCT
GCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCT
GAAAGTTGTATTATAGATGCGTTACATCAATAAACATTCTGTTACTTCAATAGTTTGTAAAGAAAACG
TGGGGCCAAGTGACGCCGCCGCAAAGCGACGCACCTGAAATCCCACAGTAAGTGCACAGGTGTAGAGT
GGATGAGCAGGGCGTGCACATGCCTCGGAAGGCCAGCTACAACCCATGTCAAAACTCGTTAATGATCC
TTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCA。
The condition of solid slant culture is: on the slant medium from the preservation bacterial classification inoculation to preparation, 20～30 ℃ of temperature were cultivated 160～240 hours.
Slant medium in per 1000 milliliters of major ingredient is: potato 100～300 grams, glucose 10～30 grams and agar 15～25 grams, pH nature.
The condition of table concentration seed culture is: behind the bacterial classification solid slant culture 160～240, mycelium is inoculated in the triangular flask that liquid seed culture medium is housed, and 20～30 ℃ of cultivations, rotating speed 150～200rpm cultivated 62～96 hours.
Seed culture medium in per 1000 milliliters of major ingredient is: glucose 10～60 grams, peptone 5～30 grams, yeast powder or yeast extract paste 3～20 grams, Fructus Hordei Germinatus leach powder 3～20 grams, pH nature.
The method of fermentor cultivation is, the liquid seeds shaking table is cultivated after 62～96 hours and is seeded in the fermentor tank that fermention medium is housed, inoculum size 5～15% (V/V), maintenance jar temperature 20～30 ℃, tank pressure a 0.4～0.8kg/cm, stir speed (S.S.) 100～200rpm, air flow 1: 0.3～1: 1.2, fermentation time 120～200 hours.
Fermention medium from per 1000 milliliters of meter major ingredient is: glucose 20～80 grams, peptone 10～40 grams, yeast powder or yeast extract paste 5～20 grams, MgSO
40.2～1 gram and KH
2PO
40.2～1 gram, the pH value of substratum is 4.0～7.0.
Fermentor cultivation adopts liquid submerged aerobic fermentation.
Jar standard of putting of the present invention is the fermented liquid thickness that becomes, and microscopy finds that mycelium begins to wear out.
Warp compares with other culture process, validation trial, and effect of the present invention is remarkable:
(1) seed culture medium provides the mycelium required nutritive substance of growing fast in a short time among the present invention, cultivates can carry out submerged fermentation in about 62 hours.
(2) liquid submerged aerobic fermentation base among the present invention, the nutritive ingredient reasonable ratio, can guarantee the growth needs of Fomes fomentarius, the substratum material is abundant, only need are once prepared burden in the whole batch fermentation process, once sterilization gets final product, have save time, the saving of labor, reduced again because the reinforced midway chance that causes microbiological contamination.
(3) adopt the deep liquid aerobic fermentation technology among the present invention to carry out batch fermentation, every liter of fermented liquid can obtain the dried mycelium of 10～15 grams.
(4) the present invention domesticly studies the zymotechnique of Fomes fomentarius first, and experimental study and the preparation of industrialization later for this bacterium all have very big directive significance.
Embodiment
Above-mentioned Fomes fomentarius (Fomes fomentarius) is to separate to obtain from the wild wooden hoof entity that gather in China Changbai mountain, Jilin mountain area.Now be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is: CGMCC No.1658 belongs to Basidiomycetes (Basidiomycetes), Aphyllophorales (Polyporales), polyporaceae (Polyporaceae), shelf fungus belongs to (Fomes).
Below in conjunction with specific embodiment the present invention is described in further detail.
Embodiment 1: the separation and Culture of bacterial strain
In the laboratory,, can obtain Fomes fomentarius of the present invention according to following condition separation and Culture.The wooden hoof entity of gathering from China Changbai mountain, Jilin mountain area, 0.1% mercury chloride disinfecting surface 3min, rinsed with sterile water is placed on wipe dry on the aseptic filter paper, with sterile razor blade cut-out exodermis, picking one little block organization moves and receives 2% and contain on the water agar plate of 100 μ g/ml penbritins and 100 μ g/ml Streptomycin sulphates.Under 20 ℃～30 ℃ temperature, cultivate, treat that mycelia grows after, the mycelia top moved receives on the PDA substratum, repeat purifying continuously 3～5 times, obtain pure culture.
Embodiment 2: the preparation of slant medium
(1) gets fresh peeling potato 100 grams, be cut into the cubic block about 0.5 cubic centimetre, water flushing immediately, boil 20min with 1000 ml distilled waters, filtered through gauze is got filtrate and is added 15 gram agar and 10 gram glucose heating for dissolving, add water and be settled to 1000 milliliters, be slant medium after the sterilization.
(2) get fresh peeling potato 200 grams, be cut into the cubic block about 0.5 cubic centimetre, water flushing immediately, boil 20min with 1000 ml distilled waters, filtered through gauze is got filtrate and is added 20 gram agar and 20 gram glucose heating for dissolving, add water and be settled to 1000 milliliters, be slant medium after the sterilization.
(3) get fresh peeling potato 300 grams, be cut into the cubic block about 0.5 cubic centimetre, water flushing immediately, boil 20min with 1000 ml distilled waters, filtered through gauze is got filtrate and is added 25 gram agar and 30 gram glucose heating for dissolving, add water and be settled to 1000 milliliters, be slant medium after the sterilization.
Embodiment 3: the preparation of seed culture medium
(1) gets glucose 10 grams, peptone 5 grams, yeast powder (cream) 3 grams, Fructus Hordei Germinatus leaching powder 3 grams,, add water and be settled to 1000 milliliters, be seed culture medium after the sterilization each component mixed dissolution.
(2) get glucose 30 grams, peptone 20 grams, yeast powder (cream) 10 grams, Fructus Hordei Germinatus leaching powder 10 grams,, add water and be settled to 1000 milliliters, be seed culture medium after the sterilization each component mixed dissolution.
(3) get glucose 60 grams, peptone 30 grams, yeast powder (cream) 20 grams, Fructus Hordei Germinatus leaching powder 20 grams,, add water and be settled to 1000 milliliters, be seed culture medium after the sterilization each component mixed dissolution.
Embodiment 4: the preparation of liquid submerged fermentation substratum
(1) gets glucose 20 grams, peptone 10 grams, yeast powder (cream) 5 grams, MgSO
40.2 gram, KH
2PO
40.2 gram.With mentioned component mixing and water adding to 1000 milliliter, add 0.6 milliliter of bubble enemy in addition and make defoamer, transfer pH to 4.0, be fermention medium after the sterilization.
(2 get glucose 40 grams, peptone 20 grams, yeast powder (cream) 10 grams, MgSO
40.5 gram, KH
2PO
40.5 gram.With mentioned component mixing and water adding to 1000 milliliter, add 0.6 milliliter of bubble enemy in addition and make defoamer, transfer pH to 5.0, be fermention medium after the sterilization.
(3) get glucose 80 grams, peptone 40 grams, yeast powder (cream) 20 grams, MgSO
41 gram, KH
2PO
41 gram.With mentioned component mixing and water adding to 1000 milliliter, add 0.6 milliliter of bubble enemy in addition and make defoamer, transfer pH to 7.0, be fermention medium after the sterilization.
Embodiment 5: deep liquid cultural method one
(1) get the fresh slant medium of preparing by embodiment 2, the mycelium of picking preservation bacterial classification adopts the method for coating or line to be inoculated on the slant medium, cultivates 240 hours in 20 ℃.
(2) select mycelium slant culture bacterial classification in great numbers, from last picking mycelium or take lawn and be inoculated in and be equipped with, under 20 ℃ of temperature, rotating speed 150rpm condition, cultivated 96 hours by the shaking the bottle of the liquid seed culture medium of embodiment 3 preparation.
(3) get the inoculum that is in the logarithmic growth middle and later periods, transfer with the inoculum size of 15% (V/V) and to be equipped with in the fermentor tank by the fermention medium of embodiment 4 preparation, keep jar temperature 20 ℃, a tank pressure 0.5kg/cm, stir speed (S.S.) 100rpm, air flow 1: 0.3, fermented incubation time 200 hours.It is thick to treat that fermented liquid becomes sticky, and when microscopy observation mycelium begins to wear out, stops fermentation, puts jar, collects mycelium and fermented liquid respectively, and every liter of fermented liquid can obtain the above dry mycelium of 10g.
Embodiment 6: deep liquid cultural method two
(1) get the fresh slant medium of preparing by embodiment 2, the mycelium of picking preservation bacterial classification adopts the method for coating or line to be inoculated on the slant medium, cultivates 200 hours in 25 ℃.
(2) select mycelium slant culture bacterial classification in great numbers, from last picking mycelium or take lawn and be inoculated in and be equipped with,, cultivated 62 hours under the rotating speed 180rpm condition 25 ℃ of temperature by the shaking the bottle of the liquid seed culture medium of embodiment 3 preparation.
(3) get the inoculum that is in the logarithmic growth middle and later periods, transfer with the inoculum size of 5% (V/V) and to be equipped with in the fermentor tank by the fermention medium of embodiment 4 preparation, keep jar temperature 25 ℃, a tank pressure 0.6kg/cm, stir speed (S.S.) 150rpm, air flow 1: 0.6, fermented incubation time 120 hours.It is thick to treat that fermented liquid becomes sticky, and when microscopy observation mycelium begins to wear out, stops fermentation, puts jar, collects mycelium and fermented liquid respectively, and every liter of fermented liquid can obtain the above dry mycelium of 10g.
Embodiment 7: deep liquid cultural method three
(1) get the fresh slant medium of preparing by embodiment 2, the mycelium of picking preservation bacterial classification adopts the method for coating or line to be inoculated on the slant medium, cultivates 160 hours in 30 ℃.
(2) select mycelium slant culture bacterial classification in great numbers, from last picking mycelium or take lawn and be inoculated in and be equipped with,, cultivated 80 hours under the rotating speed 200rpm condition 30 ℃ of temperature by the shaking the bottle of the liquid seed culture medium of embodiment 3 preparation.
(3) get the inoculum that is in the logarithmic growth middle and later periods, transfer with the inoculum size of 10% (V/V) and be equipped with in the fermentor tank of the fermention medium for preparing by embodiment 4, keep 30 ℃ of jar temperature, tank pressure 0.8kg/cm, stir speed (S.S.) 200rpm, air flow 1: 1.2, fermentation culture is about 180 hours.It is thick to treat that fermented liquid becomes sticky, and when microscopy observation mycelium begins to wear out, stops fermentation, puts jar, collects mycelium and fermented liquid respectively, and every liter of fermented liquid can obtain the above dry mycelium of 10g.
At last, it is also to be noted that what more than enumerate only is specific embodiments of the invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.
The present invention can summarize with other the specific form without prejudice to spirit of the present invention and principal character.Therefore, no matter from which point, above-mentioned embodiment of the present invention all can only be thought can not limit the present invention to explanation of the present invention, claims have been pointed out scope of the present invention, and scope of the present invention is not pointed out in above-mentioned explanation, therefore, in implication suitable and any change in the scope, all should think to be included in the scope of claims with claims of the present invention.
Claims (10)
1, a kind of Fomes fomentarius (Fomes fomentarius), it is characterized in that, be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is: CGMCC No.1658, belong to Basidiomycetes (Basidiomycetes), Aphyllophorales (Polyporales), polyporaceae (Polyporaceae), shelf fungus belongs to (Fomes).
2, a kind of liquid submerged fermentation prepares the method for Fomes fomentarius thalline, it is characterized in that, the Fomes fomentarius bacterial strain that separation and purification obtains from the wild wooden hoof entity, be the bacterial classification that sets out with this bacterial strain, through solid slant culture, table concentration seed culture, fermentor cultivation, prepare Fomes fomentarius hyphostroma successively.
3、2,：DNA：CCTCCGCTTATTGATATGCTTAAGTTCAGCGGGTAGTCCTACCTGATTTGAGGTCAGAGTTCATAAAAGCTGTCTCTGACGAGACCATTAGAAGCTCTCCAAACGCTTCACGGTCGCGGCGTAGACATTATCACACCGACAGCCGATCCGCAAGGAACCAAGCTAATGCATTTGAGAGGAGCTGACTCAAACGAGGGCCAGCAAAAGCCTCCAATAAGCCAACCCTACAAACCCGCAAAGGTTTATAGGTTGAGAATTTCATGACACTCAAACAGGCATGCTCCTCGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGATTCGATGATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTGTATTATAGATGCGTTACATCAATAAACATTCTGTTACTTCAATAGTTTGTAAAGAAAACGTGGGGCCAAGTGACGCCGCCGCAAAGCGACGCACCTGAAATCCCACAGTAAGTGCACAGGTGTAGAGTGGATGAGCAGGGCGTGCACATGCCTCGGAAGGCCAGCTACAACCCATGTCAAAACTCGTTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCA。
4. a kind of liquid submerged fermentation according to claim 2 prepares the method for Fomes fomentarius thalline, it is characterized in that, the condition of described solid slant culture is: on the slant medium from the preservation bacterial classification inoculation to preparation, 20～30 ℃ of temperature were cultivated 160～240 hours.
5, the method for preparing Fomes fomentarius hyphostroma according to the described liquid submerged fermentation of claim 4, it is characterized in that, described slant medium in per 1000 milliliters of major ingredient is: potato 100～300 grams, glucose 10～30 grams and agar 15～25 grams, pH nature.
6, a kind of liquid submerged fermentation according to claim 2 prepares the method for Fomes fomentarius thalline, it is characterized in that, the condition of described table concentration seed culture is: behind the bacterial classification solid slant culture 160～240, mycelium is inoculated in the triangular flask that liquid seed culture medium is housed, 20～30 ℃ of cultivations, rotating speed 150～200rpm cultivated 62～96 hours.
7, a kind of liquid submerged fermentation according to claim 6 prepares the method for Fomes fomentarius thalline, it is characterized in that, described seed culture medium in per 1000 milliliters of major ingredient is: glucose 10～60 grams, peptone 5～30 grams, yeast powder or yeast extract paste 3～20 grams, Fructus Hordei Germinatus leach powder 3～20 grams, pH nature.
8, a kind of liquid submerged fermentation according to claim 2 prepares the method for Fomes fomentarius thalline, it is characterized in that, the method of described fermentor cultivation is, the liquid seeds shaking table is cultivated after 62～96 hours and is seeded in the fermentor tank that fermention medium is housed, inoculum size 5～15% (V/V), maintenance jar temperature 20～30 ℃, tank pressure a 0.4～0.8kg/cm, stir speed (S.S.) 100～200rpm, air flow 1: 0.3～1: 1.2, fermentation time 120～200 hours.
9, a kind of liquid submerged fermentation according to claim 1 prepares the method for Fomes fomentarius thalline, it is characterized in that described fermention medium from per 1000 milliliters of meter major ingredient is: glucose 20～80 grams, peptone 10～40 grams, yeast powder or yeast extract paste 5～20 grams, MgSO
40.2～1 gram and KH
2PO
40.2～1 gram, the pH value of substratum is 4.0～7.0.
10, a kind of liquid submerged fermentation according to claim 2 prepares the method for Fomes fomentarius thalline, it is characterized in that, described fermentor cultivation adopts liquid submerged aerobic fermentation.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102405767A (en) * | 2011-08-31 | 2012-04-11 | 汤阴县食用菌研究所 | Domestication cultivating process of wild fomes fomentarius |
| CN107523507A (en) * | 2017-09-04 | 2017-12-29 | 北方民族大学 | A kind of culture medium and its cultural method of tinder fungus submerged fermentation |
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| CN114699343A (en) * | 2022-01-17 | 2022-07-05 | 浙江长生鸟健康科技股份有限公司 | Phellinus linteus fermentation product and preparation method thereof |
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| JPH0725573A (en) * | 1992-02-13 | 1995-01-27 | Toshiba Erebeeta Technos Kk | Escalator remote monitoring device |
| CN100346796C (en) * | 2000-01-12 | 2007-11-07 | 有限会社生命科学研究所 | Phy siologically active substance EEM-S originating in mushrooms, process for producing same and drugs |
| CN1286475C (en) * | 2004-12-10 | 2006-11-29 | 陈康林 | Chinese medicine for treating liver disease |
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2006
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102405767A (en) * | 2011-08-31 | 2012-04-11 | 汤阴县食用菌研究所 | Domestication cultivating process of wild fomes fomentarius |
| CN107523507A (en) * | 2017-09-04 | 2017-12-29 | 北方民族大学 | A kind of culture medium and its cultural method of tinder fungus submerged fermentation |
| CN112703967A (en) * | 2020-12-07 | 2021-04-27 | 贵州大学 | Method for preparing wood rot fungus liquid strain by using yellow serofluid and bean dregs |
| CN114699343A (en) * | 2022-01-17 | 2022-07-05 | 浙江长生鸟健康科技股份有限公司 | Phellinus linteus fermentation product and preparation method thereof |
| CN114699343B (en) * | 2022-01-17 | 2024-03-01 | 浙江长生鸟健康科技股份有限公司 | Phellinus linteus fermentation product and preparation method thereof |
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