CN1243092C - Liquid nutrient medium for artificial cultural ganoderma lucidum and cultivation method - Google Patents

Liquid nutrient medium for artificial cultural ganoderma lucidum and cultivation method Download PDF

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Publication number
CN1243092C
CN1243092C CN 03146984 CN03146984A CN1243092C CN 1243092 C CN1243092 C CN 1243092C CN 03146984 CN03146984 CN 03146984 CN 03146984 A CN03146984 A CN 03146984A CN 1243092 C CN1243092 C CN 1243092C
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cultivating
grams
nutrient medium
liquid nutrient
present
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CN 03146984
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CN1600848A (en
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吴佛煌
吴海青
黄达宏
余德武
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Priority to CN 03146984 priority Critical patent/CN1243092C/en
Priority to PCT/CN2004/001080 priority patent/WO2005030937A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The present invention relates to a method for artificially cultivating ganoderma lucidum with liquid culture media. The following components of potassium dihydrogen phosphate, ammonium sulfate, magnesium sulfate, edible sugar, glucose powder, calcium gluconate injection and vitamin B1 injection are added to distilled water. The method for artificially cultivating ganoderma lucidum with liquid culture media of the present invention has the advantages of few required components, simple preparation, low cost, convenient factory production, easy management, low cost, no environment pollution and high product quality. The method for cultivating ganoderma lucidum with liquid culture media, which is adopted by the present invention, not only has simple techniques and equipment, but also takes short cultivation time which is half of the cultivation time of the methods of bag material cultivation and basswood cultivation. Annual effective cultivation time is increased. The product of the present invention belongs to the green environmental protection products. The present invention is a method for cultivating ganoderma lucidum with liquid culture media, which has obvious social benefits and economic benefits.

Description

Liquid nutrient medium of a kind of artificial culture glossy ganoderma and cultivating method thereof
1, technical field:
The present invention relates to liquid nutrient medium and the cultivating method thereof of artificial culture glossy ganoderma, belong to the liquid nutrient medium of artificial culture glossy ganoderma and the innovative technology of cultivating method thereof.
2, background technology:
Glossy ganoderma belongs to Mycophyta, under the specific environment that is grown in the Nature of natural glossy ganoderma, is the rare medicinal herbs in China's Chinese medicinal materials.Because natural glossy ganoderma rareness, be difficult to satisfy people's demand, so the insider carries out artificial culture by every means, the method of existing artificial culture glossy ganoderma has cultivating in bag and segment wood cultivated two kinds, also do not adopt the method for liquid nutrient medium success cultivating ganoderma, but the shortcoming that cultivating in bag and segment wood cultivated two kinds of methods exist is that incubation time is long, and is subjected to the restriction of season and culture environment, the culture condition harshness, and must expend a large amount of timber, the cost height, and the place that must take is big, also need adopt the agricultural chemicals deinsectization, contaminate environment not only, but also cause that remaining agricultural chemicals composition is arranged in the glossy ganoderma, therefore, people are devoted to study the method that adopts the liquid nutrient medium cultivating ganoderma.
3, summary of the invention:
The liquid nutrient medium that the objective of the invention is to consider the problems referred to above and a kind of artificial culture glossy ganoderma is provided.
Another object of the present invention is to provide a kind of method that adopts the liquid nutrient medium cultivating ganoderma.The present invention not only technology and equipment is simple, and incubation time is short, and it is low to cultivate cost than cultivating in bag and segment wood cultivated method; And it is the quality product height, and free from environmental pollution.The present invention is the method that a kind of social benefit and economic benefit all adopt the liquid nutrient medium cultivating ganoderma significantly.
The liquid nutrient medium of the artificial cultivating ganoderma of the present invention adds following composition in distilled water:
1) potassium primary phosphate;
2) ammonium sulfate;
3) sal epsom;
4) table sugar;
5) glucose powder;
6) calcium gluconate injection;
7) vitamins B 1Injection.
The weight that above-mentioned each composition contains in per 1000 ml distilled waters is:
1) potassium primary phosphate 3～11 grams;
2) ammonium sulfate 2～8 grams;
3) sal epsom 0.6～4 gram;
4) table sugar 20～60 grams;
5) glucose powder 15～65 grams;
6) calcium gluconate injection 10～50 grams;
7) vitamins B 1Injection 2～8 grams.
The cultivating method of artificial culture glossy ganoderma includes following steps:
1) configuration liquid nutrient medium;
2) liquid nutrient medium is packed into cultivating container;
3) cultivating container that liquid nutrient medium will be housed is sterilized;
4) glossy ganoderma is seeded in the cultivating container;
5) place cultivating chamber nontoxic, pollution-free, that do not have other bacterial classifications to cultivate cultivating container after the inoculation, glossy ganoderma is grown in the cultivating container.
The temperature of above-mentioned cultivating chamber is controlled at 27～30 ℃.
The liquid nutrient medium of the artificial cultivating ganoderma of the present invention, its required component is few, is equipped with simply, and cost is low.
The present invention adopts the method for liquid nutrient medium cultivating ganoderma, and not only technology and equipment are simple for it, and incubation time is short, be half of cultivating in bag and segment wood cultivated method incubation time, annual effective cultivation increased frequency is convenient to batch production production, be easy to management, cost is low, and free from environmental pollution; And the quality product height, belong to Green Product.The present invention is the method that a kind of social benefit and economic benefit all adopt the liquid nutrient medium cultivating ganoderma significantly.
4, embodiment:
Embodiment 1:
The liquid nutrient medium of the artificial cultivating ganoderma of the present invention adds following composition in distilled water:
1) potassium primary phosphate;
2) ammonium sulfate;
3) sal epsom;
4) table sugar;
5) glucose powder;
6) calcium gluconate injection;
7) vitamins B 1Injection.
In the present embodiment, table sugar is a sucrose, and the weight that above-mentioned each composition contains in per 1000 ml distilled waters is:
1) potassium primary phosphate 10 grams;
2) ammonium sulfate 7 grams;
3) sal epsom 1 gram;
4) sucrose 25 grams;
5) glucose powder 45 grams;
6) calcium gluconate injection 20 grams;
7) vitamins B 1Injection 3 grams.
The cultivating method of artificial culture glossy ganoderma includes following steps:
1) configuration liquid nutrient medium;
2) liquid nutrient medium is packed into cultivating container;
3) cultivating container that liquid nutrient medium will be housed is sterilized;
4) glossy ganoderma is seeded in the cultivating container;
5) place cultivating chamber nontoxic, pollution-free, that do not have other bacterial classifications to cultivate cultivating container after the inoculation, glossy ganoderma is grown in the cultivating container.
The temperature of above-mentioned cultivating chamber is controlled at 27～30 ℃.
Embodiment 2:
The cultivating method of present embodiment artificial culture glossy ganoderma is identical with embodiment 1, what difference was that each composition of liquid nutrient medium of artificial culture glossy ganoderma contains in per 1000 ml distilled waters varies in weight, and the weight that its each composition contains in per 1000 ml distilled waters is:
1) potassium primary phosphate 8 grams;
2) ammonium sulfate 6 grams;
3) sal epsom 3 grams;
4) sucrose 55 grams;
5) glucose powder 30 grams;
6) calcium gluconate injection 40 grams;
7) vitamins B 1Injection 6 grams.
Embodiment 3:
The cultivating method of present embodiment artificial culture glossy ganoderma is identical with embodiment 1, what difference was that each composition of liquid nutrient medium of artificial culture glossy ganoderma contains in per 1000 ml distilled waters varies in weight, and the weight that its each composition contains in per 1000 ml distilled waters is:
1) potassium primary phosphate 4 grams;
2) ammonium sulfate 6 grams;
3) sal epsom 3 grams;
4) sucrose 45 grams;
5) glucose powder 35 grams;
6) calcium gluconate injection 35 grams;
7) vitamins B 1Injection 7 grams.

Claims (4)

1, the liquid nutrient medium of a kind of artificial culture glossy ganoderma is characterized in that being made up of following composition:
1) potassium primary phosphate;
2) ammonium sulfate;
3) sal epsom;
4) table sugar;
5) glucose powder;
6) calcium gluconate injection;
7) vitamins B 1Injection;
8) distilled water.
2, the liquid nutrient medium of artificial culture glossy ganoderma according to claim 1 is characterized in that the weight that above-mentioned each composition contains is in per 1000 ml distilled waters:
1) potassium primary phosphate 3～11 grams;
2) ammonium sulfate 2～8 grams;
3) sal epsom 0.6～4 gram;
4) table sugar 20～60 grams;
5) glucose powder 15～65 grams;
6) calcium gluconate injection 10～50 grams;
7) vitamins B 1Injection 2～8 grams.
3, the cultivating method of a kind of artificial culture glossy ganoderma is characterized in that including following steps:
1) the described liquid nutrient medium of configuration claim 1;
2) liquid nutrient medium is packed into cultivating container;
3) cultivating container that liquid nutrient medium will be housed is sterilized;
4) glossy ganoderma is seeded in the cultivating container;
5) place cultivating chamber nontoxic, pollution-free, that do not have other bacterial classifications to cultivate cultivating container after the inoculation, glossy ganoderma is grown in the cultivating container.
4, the cultivating method of artificial culture glossy ganoderma according to claim 3 is characterized in that the temperature of above-mentioned cultivating chamber is controlled at 27～30 ℃.
CN 03146984 2003-09-28 2003-09-28 Liquid nutrient medium for artificial cultural ganoderma lucidum and cultivation method Expired - Fee Related CN1243092C (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN 03146984 CN1243092C (en) 2003-09-28 2003-09-28 Liquid nutrient medium for artificial cultural ganoderma lucidum and cultivation method
PCT/CN2004/001080 WO2005030937A1 (en) 2003-09-28 2004-09-23 The liquid medium and culture method for artificial cultivation of ganoderma lucidum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 03146984 CN1243092C (en) 2003-09-28 2003-09-28 Liquid nutrient medium for artificial cultural ganoderma lucidum and cultivation method

Publications (2)

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CN1600848A CN1600848A (en) 2005-03-30
CN1243092C true CN1243092C (en) 2006-02-22

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WO (1) WO2005030937A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100433966C (en) * 2006-06-28 2008-11-19 中山大学 Method for improving quality of Ganoderma lucidium spore

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101560118B (en) * 2009-02-26 2012-07-04 浙江大学 Novel culture medium formulation of glossy ganoderma mycelium fermentation and optimization method
CN101665378B (en) * 2009-09-04 2011-09-28 郭欣 Mother seed carrier culture medium for culturing natural ganoderma and culturing method
CN106258473A (en) * 2015-05-20 2017-01-04 镇江市都市生态农业有限公司 The cultural method of Ganoderma
CN108770594A (en) * 2018-05-18 2018-11-09 吴海青 A kind of breeding method of the fresh and alive rich germanium polysaccharide ganoderma lucidum of individual

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100433966C (en) * 2006-06-28 2008-11-19 中山大学 Method for improving quality of Ganoderma lucidium spore

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CN1600848A (en) 2005-03-30

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