CN104845892A - R.vinctus and application thereof in promoting aquilaria plants to produce agilawood - Google Patents
R.vinctus and application thereof in promoting aquilaria plants to produce agilawood Download PDFInfo
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Abstract
The invention provides R.vinctus and an application thereof in promoting aquilaria plants to produce agilawood. The R.vinctus provided by the invention belongs to rigidoporus, meripilaceae, polyporales, agaricomycetes, basidiomycota, fungi. The R.vinctus provided by the invention is obtained by separating from aquilaria sinensis and is used for promoting the aquilaria with aquilaria sinensis as a representative to produce the agilawood, and a formed agilawood layer is relatively thick and has large penetration surface. The R.vinctus provided by the invention can improve the quality of the agilawood, and has great significance to production of the agilawood.
Description
Technical field
The present invention relates to microorganism and microbe application field, be specifically related to a kind of Rigidoporus durus (Rigidoporus vinctus) and promote that eaglewood produces the application of agalloch eaglewood.
Background technology
Agalloch eaglewood is the timber that thymelaeceae (Thymelaeaceae) agalloch eaglewood genus (Aquilaria) plant contains resin, the formation of agalloch eaglewood is very special, healthy Lignum Aquilariae Resinatum can not produce agalloch eaglewood class material, just induces Edgeworthia chrysantha after being only subject to the extraneous injury such as physical injury, chemistry injury and fungal infection.The agalloch eaglewood that quality is high needs even can be formed decades for several years.Due to natural agilawood formed singularity and the Edgeworthia chrysantha cycle long, so Edgeworthia chrysantha technology becomes the focus of research.
To the research of Lignum Aquilariae Resinatum microorganism, this problem is by finding that the formation of fungi to agalloch eaglewood plays an important role, but the fungal bacterial strain of existing report and the fungi Edgeworthia chrysantha of urging fragrant method apart from short, penetrativity is limited, making it can not widespread use, has great significance so find the bacterial strain that can improve agalloch eaglewood quality to be applied to production.
Summary of the invention
For the problems referred to above, the invention provides and a kind ofly can promote the formation of agalloch eaglewood and the large bacterial strain of agalloch eaglewood diffusingsurface formed and application thereof, this bacterial strain pulvis or paste, bacterial strain fermentation liquor, degerming after filtrate and bacterium liquid/filtrate concentrate formulation can promote eaglewood Edgeworthia chrysantha.
First aspect present invention provides a kind of Rigidoporus fungi and is promoting that agalloch eaglewood belongs to the application in (Aquilaria), plan agalloch eaglewood genus (Gyrinops) or other inducible plants generation agalloch eaglewood, described Rigidoporus is in mycota (Fungi), Basidiomycota (Basidiomycota), agaric steel (Agaricomycetes), Rigidoporus durus order (Polyporales), Antrodia section (meripilaceae), Rigidoporus (Rigidoporus).
The advantage of Rigidoporus fungi provided by the invention is: Edgeworthia chrysantha bacterial strain connects bacterium can form thicker agalloch eaglewood layer and penetration surface is large after 2 months.
Preferably, described Rigidoporus fungi is Rigidoporus durus (R.vinctus).
Further preferably, described Rigidoporus durus (R.vinctus) is the Rigidoporus durus (R.vinctus) as described in second aspect.
As described in the present invention, can exchange between described " Rigidoporus durus (R.vinctus) " and " Rigidoporus durus (Rigidoporus vinctus) ".
As described in the present invention, described " Rigidoporus durus (Rigidoporus vinctus) ASAF02 " and Rigidoporus durus (R.vinctus ASAF02) " between can exchange.
Second aspect present invention provides a kind of Rigidoporus durus (Rigidoporus vinctus), and it at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center is: CGMCC NO.9594.
This strain Rigidoporus durus (Rigidoporus vinctus) of Late Cambrian of the present invention can promote the formation of agalloch eaglewood and the agalloch eaglewood diffusingsurface formed is large, the industrialization of agalloch eaglewood is had great significance, by its called after Rigidoporus durus (Rigidoporus vinctus) ASAF02.
Rigidoporus durus of the present invention (R.vinctus) ASAF02 is separated and obtains from the Edgeworthia chrysantha layer of Lignum Aquilariae Resinatum, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 9th, 2014 and (be called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, Institute of Microorganism, Academia Sinica), preserving number is: CGMCC NO.9594.
The morphological specificity of Rigidoporus durus (R.vinctus) ASAF02 on PDA substratum is: on the substratum of PDA, bacteria colony white, homogeneous shape, neat in edge; Mycelia is very thin, has dichotomy, not easily produces spore.The ITS length of Rigidoporus durus (R.vinctus) ASAF02, its rDNA is 620bp, and sequence is the nucleotide sequence shown in sequence in sequence table 3.
Third aspect present invention additionally provides Rigidoporus durus (Rigidoporus vinctus) and is promoting that agalloch eaglewood belongs to the application in (Aquilaria) plant generation agalloch eaglewood.
Preferably, described Rigidoporus durus (R.vinctus) is the Rigidoporus durus (R.vinctus) as described in second aspect.
Fourth aspect present invention provide at least one in a kind of Rigidoporus durus (R.vinctus) bacterial strain, mycelia (be preferably mycelia pulvis or mycelia paste), the filtrate of the degerming acquisition of bacterial strain fermentation liquor, fermented liquid, bacterial strain fermentation liquor/bacterial strain fermentation liquor filtrate concentrate formulation as promoting agalloch eaglewood to belong to (Aquilaria), intend agalloch eaglewood and belong to application in the Edgeworthia chrysantha agent of (Gyrinops) or other inducible plant Edgeworthia chrysanthas.
Preferably, described Rigidoporus durus (R.vinctus) is the Rigidoporus durus (R.vinctus) as described in second aspect.
Fifth aspect present invention provides a kind of Edgeworthia chrysantha agent, and its activeconstituents comprises at least one in Rigidoporus durus (R.vinctus) bacterial strain, mycelia (be preferably mycelia pulvis or mycelia paste), the filtrate of the degerming acquisition of bacterial strain fermentation liquor, fermented liquid, bacterial strain fermentation liquor/bacterial strain fermentation liquor filtrate concentrate formulation.
Preferably, described Rigidoporus durus (R.vinctus) is the Rigidoporus durus (R.vinctus) as described in second aspect.
As described in the present invention, " activeconstituents in degerming rear filtrate " includes but not limited to as preparing by the following method: the separating and purifying method adopting this area routine, the composition that can be used as short pastil extracted, described short pastil is the Edgeworthia chrysantha agent promoting that agalloch eaglewood belongs to (Aquilaria), plan agalloch eaglewood belongs to (Gyrinops) or other inducible plant Edgeworthia chrysanthas.
As described in the present invention, it is cultivate in the liquid nutrient medium of 15-35 DEG C that the preparation method of " bacterial strain fermentation liquor " to be preferably Rigidoporus durus (R.vinctus) (being preferably ASAF02) inoculation in temperature, obtains bacterial strain fermentation liquor.The pH value of described liquid nutrient medium can be pH3-11; Concrete substratum can be liquid PDA substratum, and the time of cultivation can be 3-7 days, and the mode of cultivation can be cultivated for shaking table.The temperature of cultivating is specifically as follows 30 DEG C.
As described in the present invention, described " bacterial strain fermentation liquor/bacterial strain fermentation liquor filtrate concentrate formulation " expression " bacterial strain fermentation liquor concentrate formulation or bacterial strain fermentation liquor degerming after filtrate concentrate formulation ".
As described in the present invention, described mycelia can be prepared into mycelia pulvis or paste.
Sixth aspect present invention provides a kind of method that agalloch eaglewood genus (Aquilaria), plan agalloch eaglewood genus (Gyrinops) or other inducible plants produce agalloch eaglewood that promotes, is the Edgeworthia chrysantha agent described in fifth aspect present invention is inoculated in agalloch eaglewood to belong on (Aquilaria) roots of plants, stem and/or branch.
The present invention utilizes Rigidoporus durus (R.vinctus) bacterial strain, mycelia (being preferably mycelia pulvis or mycelia paste), bacterial strain fermentation liquor, degerming filtrate, bacterial strain fermentation liquor/bacterial strain fermentation liquor filtrate concentrate formulation to promote Edgeworthia chrysantha, and concrete technical scheme is: Rigidoporus durus (R.vinctus) bacterial strain, mycelia (being preferably mycelia pulvis or mycelia paste), bacterial strain fermentation liquor, degerming filtrate, bacterial strain fermentation liquor/filtrate concentrate formulation are inoculated on whitewood cus-cus, stem or branch as at least one in Edgeworthia chrysantha agent.
Preferably, above-mentioned vaccination ways is rossing inoculation, the inoculation of entire body Edgeworthia chrysantha technology or vaccination ways of getting into the cave.
Described entire body Edgeworthia chrysantha technology inoculation is preferably: the tree that at least one will inoculated in the filtrate after Rigidoporus durus (Rigidoporus vinctus) zymocyte liquid, fermented liquid removal thalline, bacterial strain fermentation liquor concentrate formulation, bacterial strain fermentation liquor filtrate concentrate formulation directly uses entire body Edgeworthia chrysantha method input agalloch eaglewood to belong to injects agalloch eaglewood genus (Aquilaria) plant by infusion set, utilize the transpiration of plant to dredge to each organs such as plant stem, branch, roots, impel the inner Edgeworthia chrysantha of whole plant; Described inoculation of getting into the cave is got into the cave to position, 50cm-1m place, ground from root for belonging to (Aquilaria) plant agalloch eaglewood to be seeded, hole is deep to 3-5cm, and the activeconstituents in above-mentioned inoculation Rigidoporus durus (Rigidoporus vinctus) bacterial strain, mycelia (be preferably mycelia pulvis or mycelia paste), bacterial strain fermentation liquor, degerming filtrate, filtrate, at least one in bacterial strain fermentation liquor/bacterial strain fermentation liquor filtrate concentrate formulation are injected hole.Further preferably, in described entire body Edgeworthia chrysantha technology vaccination ways, on the bacterium liquid of gained and the bacteria-free filtrate tree directly with entire body Edgeworthia chrysantha method input agalloch eaglewood genus.
Preferably, described Rigidoporus durus (R.vinctus) is the Rigidoporus durus (R.vinctus) as described in second aspect.
As described in the present invention, in in described first and third, four, five, six, described agalloch eaglewood belongs to (Aquilaria) plant and includes but not limited to Aguilaria malaccensis Lamk (Aquilaria malaccensis), Vietnam agalloch eaglewood (Aquilaria crassna) or Lignum Aquilariae Resinatum (Aquilaria sinensis); Be preferably Lignum Aquilariae Resinatum (Aquilaria sinensis).The age of tree of described Lignum Aquilariae Resinatum is preferably the Lignum Aquilariae Resinatum of more than 6 years.
The advantage of Rigidoporus durus provided by the invention is: Edgeworthia chrysantha bacterial strain Rigidoporus durus (R.vinctus) (be preferably ASAF02) inoculates and can form thicker agalloch eaglewood layer after 2 months and penetration surface is large.This bacterial strain still can grow under the condition of PH2-11 and temperature 15-35 DEG C, illustrates to have very strong environment resistance.Late Cambrian Rigidoporus durus (R.vinctus) of the present invention (be preferably ASAF02) can promote Lignum Aquilariae Resinatum Edgeworthia chrysantha and to be formed and penetration surface is large, has great significance to the industrialization of agalloch eaglewood.
Accompanying drawing explanation
Fig. 1 is that Rigidoporus durus of the present invention (R.vinctus) ASAF02 bacterial strain promotes that agalloch eaglewood is formed and fungal morphology observation of characteristics; Wherein A is that Rigidoporus durus (R.vinctus) ASAF02 promotes Edgeworthia chrysantha design sketch (A1 punching place A2 distance 50cm place, aperture); B is the bacterium colony of Rigidoporus durus (Rigidoporus vinctus) ASAF02.
Rigidoporus durus (R.vinctus) ASAF02 bacterial strain that Fig. 2 is the embodiment of the present invention 3 promotes that the thin layer that Edgeworthia chrysantha institute produces agalloch eaglewood is identified; BK is healthy Lignum Aquilariae Resinatum, and ST is chromone contrast, and CK is the control medicinal material that Chinese Pharmacopoeia specifies, IW is the ASAF02 inoculation agalloch eaglewood of 2 months.
Rigidoporus durus (R.vinctus) ASAF02 bacterial strain that Fig. 3 is the embodiment of the present invention 4 promotes that the thin layer that Edgeworthia chrysantha institute produces agalloch eaglewood is identified; BK is healthy Lignum Aquilariae Resinatum, and ST is chromone contrast, and CK is the control medicinal material that Chinese Pharmacopoeia specifies, BW is the ASAF02 inoculation agalloch eaglewood of 3 months.
Rigidoporus durus (R.vinctus) ASAF02 bacterial strain that Fig. 4 is the embodiment of the present invention 5 promotes that the thin layer that Edgeworthia chrysantha institute produces agalloch eaglewood is identified; BK is healthy Lignum Aquilariae Resinatum, and ST is chromone contrast, and CK is the control medicinal material that Chinese Pharmacopoeia specifies, IW is the ASAF02 inoculation agalloch eaglewood of 70 days.
Embodiment
The following stated is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.
Without outside special instruction in the embodiment of the present invention, agents useful for same and consumptive material are commercial goods.
Method in following embodiment, if no special instructions, is ordinary method.
Percentage composition in following embodiment, if no special instructions, is mass percentage.
PDA liquid nutrient medium used in following embodiment: weigh peeled potatoes 200g, be cut into small pieces, add poach 30min, filtered through gauze, add glucose 20g, be finally settled to 1L.Solid PDA medium is: weigh peeled potatoes 200g, be cut into small pieces, add poach 30min, filtered through gauze, add glucose 20g, add 15-20g agar, be finally settled to 1L.
The separation of embodiment 1 Rigidoporus durus (R.vinctus) ASAF02 bacterial strain, screening and identification
The present invention utilizes tissue isolation to carry out strains separation.Concrete grammar is as described below: take natural agalloch eaglewood sample in copper Tieling, Behavioral Change, rinse 30min under a tap, then the alcohol disinfecting 3-5min of 70% is used, with aseptic water washing 3 times, then the clorox of 10% (mass percentage concentration) is used to soak 5min, use aseptic water washing again 3 times, the segment being then cut into 2mm-3mm is placed in the substratum containing Streptomycin sulphate, and last sterilized water compares.After 3d, every day checked and picking bacterial strain purifying, until 2 weeks.
The bacterial strain be separated to is cultivated 3-7d at PDA liquid nutrient medium, inoculated fungi mycelia or utilize entire body Edgeworthia chrysantha technology input fermented liquid, degerming filtrate, extracting solution containing the activeconstituents in filtrate screens Lignum Aquilariae Resinatum, find that there is a kind of inoculation xylem agalloch eaglewood layer clear-cut visible (in Fig. 1 A) after 2 months, Figure 1A is that Rigidoporus durus (R.vinctus) ASAF02 promotes Edgeworthia chrysantha design sketch (A1 punching place A2 distance 50cm place, aperture), is Rigidoporus durus (R.vinctus) ASAF02 by this Strain Designation.Bacterial strain Rigidoporus durus (R.vinctus) ASAF02 of this strain energy Edgeworthia chrysantha is cultivated observation on substratum, and bacterium colony is circular, white, homogeneous shape, neat in edge (in Fig. 1 B).Because this bacterial strain does not produce spore, adopt molecular method qualification, extract ASAF002 bacterial strain DNA, with ITS1 (SEQUENCE NO.1:5 '-TCCGATGGTGAACCTGCGG-3 ') and ITS4 (SEQUENCE NO.2:
5 '-TCCTCCGCTTATTGATATGC-3 ') for primer carries out pcr amplification, pcr amplification checks order, and order-checking obtains the ITS sequence of 620bp, and base sequence is as shown in SEQUENCE NO.3 in sequence table.The sequence of acquisition is carried out Blast comparison in Genbank database, and result shows its sequence and R.vinctus (Accession No.HQ400710; JQ717317) homology reaches 99%, is accredited as Rigidoporus durus (R.vinctus) ASAF02 by morphological specificity and characterization of molecules.
Rigidoporus durus (R.vinctus) ASAF02 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 9th, 2014 and (is called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, Institute of Microorganism, Academia Sinica), preserving number is: CGMCC NO.9594.
Embodiment 2 Rigidoporus durus (R.vinctus) ASAF02 strain growth condition detects.
By the mycelium inoculation of Rigidoporus durus (R.vinctus) ASAF02 in the culture dish center containing solid PDA medium, be set to respectively cultivate in the thermostat container of 10 DEG C, 15 DEG C, 20 DEG C, 25 DEG C, 28 DEG C, 30 DEG C, 35 DEG C, found that, Rigidoporus durus (R.vinctus) ASAF02 all can grow 15 DEG C to 35 DEG C conditions, but 30 DEG C of growths the most applicable.The pH value of PDA substratum is regulated with 0.1mol/L HCL and 0.1mol/L NaOH, make the liquid PDA substratum that pH value is respectively 3,4,5,6,7,8,9,10,11, mycelium inoculation is placed in the liquid PDA substratum of different pH value, cultivate at 30 DEG C of 200 revs/min of shaking tables, found that, bacterial strain all can grow at PH3 to PH11, and optimum pH is 7.
Embodiment 3 Rigidoporus durus (R.vinctus) ASAF02 promotes that agalloch eaglewood tells on checking
By Rigidoporus durus (R.vinctus) ASAF02 strain culturing 3-7d, obtain bacterial strain mycelia, fermented liquid, degerming filtrate, according to tree size by different dose inoculations, get into the cave or utilize entire body Edgeworthia chrysantha technology by the fermented liquid of mycetome, the filtrate inoculation Lignum Aquilariae Resinatum of fermented liquid removing thalline promotes Edgeworthia chrysantha, and concrete inoculation method is as follows:
1, the acquisition of inoculation material: Rigidoporus durus (R.vinctus) ASAF02 bacterial strain cultivates the mycelia obtained with liquid nutrient medium, is inoculation mycelia 1, can be made into pulvis or paste.Mycelium inoculation, in PDA liquid nutrient medium, shakes training 3-7d at 30 DEG C 200 revs/min, obtains nutrient solution, be bacterium liquid 2.After cultivating Rigidoporus durus (R.vinctus) ASAF02 bacterial strain acquisition nutrient solution according to the method described above simultaneously, filter and remove thalline, obtain degerming filtrate, be filtrate 3.The enriched material that bacterium liquid or filtrate spray concentration obtain, is concentrate formulation 4.
2, inoculate: with Lignum Aquilariae Resinatum (A.sinensis (Lour.) Gilg) for object of inoculation is inoculated, inoculation method is as follows: inoculation of 1) 1) getting into the cave: getting into the cave to position, 50cm place, ground from root of 6 years age of tree Lignum Aquilariae Resinatum, hole is deep to 3-5cm, and above-mentioned inoculation mycelia 1 or bacterium liquid 2 are injected hole respectively; Entire body Edgeworthia chrysantha technology is inoculated: on the Lignum Aquilariae Resinatum of 6 years age of trees, utilize the transpiration of plant to dredge to each organs such as plant stem, branch, roots bacterium liquid 2, filtrate 3, concentrate formulation 4 respectively respectively by infusion set, impels the inner Edgeworthia chrysantha of whole plant.Result shows, above-mentioned vaccination ways all can make Lignum Aquilariae Resinatum Edgeworthia chrysantha.Utilize get into the cave method inoculation to compare in conjunction with entire body Edgeworthia chrysantha technology with inoculation mycelia 1, bacterium liquid 2 with filtrate 3, filtrate 3 has in conjunction with entire body Edgeworthia chrysantha technology that Edgeworthia chrysantha speed is fast, Edgeworthia chrysantha face large and the feature of Edgeworthia chrysantha distance; The agalloch eaglewood that filtrate 3 also obtains in conjunction with entire body Edgeworthia chrysantha technology than the bacterium liquid 2 of same volume, concentrate formulation 4 in conjunction with entire body Edgeworthia chrysantha technology is effective.But aforesaid method all can make Lignum Aquilariae Resinatum Edgeworthia chrysantha in 2 months.
Above-mentioned inoculation operates 2 months, is cut down by the Lignum Aquilariae Resinatum of Edgeworthia chrysantha, cuts open perfume (or spice), then carries out thin layer qualification.Concrete grammar is: the agalloch eaglewood sample powder 1g getting the ASAF02 inoculation acquisition in 2 months that aforesaid method obtains is placed in tool plug triangular flask, and add methyl alcohol 25ml, supersound process 60min, filter, filtrate is settled to 25ml, as need testing solution.The wild agalloch eaglewood medicinal material (control medicinal material that Chinese Pharmacopoeia specifies, purchased from Chinese drug assay research institute, lot number: sample 121222201102) and excellent wild agalloch eaglewood (being purchased from LIGNUM AQUILARIAE SINENSIS from Hainan of China expo in 2012) and healthy Lignum Aquilariae Resinatum (stem clays into power), preparation method is the same respectively as control medicinal material solution, get 6,7-dimethoxy-2 (2-styroyl) chromone adds methyl alcohol and is mixed with every milliliter of chromone reference substance solution containing 0.05mg 6,7-dimethoxy-2 (2-styroyl) chromone.Draw each 5 μ l of need testing solution, control medicinal material solution and chromone reference substance solution, put respectively on same silica GF254 thin layer plate, with trichloromethane-ether (v ﹕ v=10 ﹕ 1) for developping agent, launch twice, taking-up is dried, inspect under putting ultra-violet lamp (254nm and 365nm), take pictures.BK in Fig. 2 is healthy Lignum Aquilariae Resinatum, ST is chromone contrast (6,7-dimethoxy-2 (2-styroyl) chromone adds methyl alcohol), CK is the control medicinal material that Chinese Pharmacopoeia specifies, IW is ASAF02 inoculation (agalloch eaglewood that filtrate 3 is infused by the entire body Edgeworthia chrysantha technology) agalloch eaglewood of 2 months.Result shows, Rigidoporus durus (R.vinctus) ASAF02 inoculation can promote the formation of agalloch eaglewood after 2 months, and wild agalloch eaglewood spot is close to (Fig. 2).
Embodiment 4 Rigidoporus durus (R.vinctus) ASAF02 promotes that Aguilaria malaccensis Lamk produces the compliance test result of agalloch eaglewood
With Aguilaria malaccensis Lamk (Aquilaria malaccensis, the age of tree is 6 years) inoculate for object of inoculation, inoculation method is with reference to the method 2 of embodiment 3) entire body Edgeworthia chrysantha result shows, above-mentioned vaccination ways can make Aguilaria malaccensis Lamk Edgeworthia chrysantha in 3 months, and wild agalloch eaglewood spot is close to (Fig. 3).BK in Fig. 3 is healthy Lignum Aquilariae Resinatum, ST is chromone contrast (6,7-dimethoxy-2 (2-styroyl) chromone adds methyl alcohol), CK is the control medicinal material that Chinese Pharmacopoeia specifies, BW is ASAF02 inoculation (agalloch eaglewood that filtrate 3 is infused by the entire body Edgeworthia chrysantha technology) agalloch eaglewood of 3 months.Result shows, Rigidoporus durus (R.vinctus) ASAF02 inoculation can promote the formation of agalloch eaglewood after 3 months, and wild agalloch eaglewood spot is close.
Embodiment 5 Rigidoporus durus (R.vinctus) ASAF02 promotes that Vietnam agalloch eaglewood produces the compliance test result of agalloch eaglewood
With Vietnam agalloch eaglewood (Aquilaria crassna, the age of tree is 6 years) inoculate for object of inoculation, inoculation method is with reference to the method 2 of embodiment 3) entire body Edgeworthia chrysantha result shows, above-mentioned vaccination ways can make Vietnam's agalloch eaglewood Edgeworthia chrysantha for 70 days, and wild agalloch eaglewood spot is close to (Fig. 4).In Fig. 4, BK is healthy Lignum Aquilariae Resinatum, ST is chromone contrast (6,7-dimethoxy-2 (2-styroyl) chromone adds methyl alcohol), CK is the control medicinal material that Chinese Pharmacopoeia specifies, IW is ASAF02 inoculation (agalloch eaglewood that filtrate 3 is infused by the entire body Edgeworthia chrysantha technology) agalloch eaglewood of 70 days.Result shows, Rigidoporus durus (R.vinctus) ASAF02 inoculation can promote the formation of agalloch eaglewood after 70 days, and wild agalloch eaglewood spot is close.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. Rigidoporus fungi is promoting that agalloch eaglewood belongs to the application in (Aquilaria), plan agalloch eaglewood genus (Gyrinops) or other inducible plants generation agalloch eaglewood, wherein, described Rigidoporus is in mycota Fungi, Basidiomycota Basidiomycota, agaric steel Agaricomycetes, Rigidoporus durus order Polyporales, Rigidoporus Rigidoporus.
2. Rigidoporus durus (R.vinctus), it is CGMCC NO.9594 at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center.
3. Rigidoporus durus (R.vinctus) is promoting that agalloch eaglewood belongs to the application in (Aquilaria), plan agalloch eaglewood genus (Gyrinops) or other inducible plants generation agalloch eaglewood.
4. at least one in the activeconstituents in the filtrate of Rigidoporus durus (R.vinctus) bacterial strain, mycelia, bacterial strain fermentation liquor, the degerming acquisition of fermented liquid, filtrate, bacterial strain fermentation liquor concentrate formulation, bacterial strain fermentation liquor filtrate concentrate formulation as promoting agalloch eaglewood to belong to (Aquilaria), intend agalloch eaglewood and belong to application in the Edgeworthia chrysantha agent of (Gyrinops) or other inducible plant Edgeworthia chrysanthas.
5. an Edgeworthia chrysantha agent, it is characterized in that, described Edgeworthia chrysantha agent comprises at least one in Rigidoporus durus (R.vinctus) bacterial strain, mycelia, bacterial strain fermentation liquor, the filtrate of the degerming acquisition of fermented liquid, bacterial strain fermentation liquor concentrate formulation, bacterial strain fermentation liquor filtrate concentrate formulation.
6. the preparation method of the bacterial strain fermentation liquor as described in claim 4 or 5 is that Rigidoporus durus (R.vinctus) to be inoculated in temperature be cultivate in the liquid nutrient medium of 15-35 DEG C, obtains bacterial strain fermentation liquor.
7. the method promoting that agalloch eaglewood belongs to (Aquilaria), plan agalloch eaglewood belongs to (Gyrinops) or other inducible plants generation agalloch eaglewood, it is characterized in that, be the Edgeworthia chrysantha agent as described in claim 4 or 5 is inoculated in agalloch eaglewood to belong on (Aquilaria), the root intending agalloch eaglewood genus (Gyrinops) or other inducible plants, stem and/or branch.
8. promotion agalloch eaglewood as claimed in claim 7 belongs to the method that (Aquilaria), plan agalloch eaglewood genus (Gyrinops) or other inducible plants produce agalloch eaglewood, it is characterized in that, described vaccination ways is rossing inoculation, the inoculation of entire body Edgeworthia chrysantha technology or vaccination ways of getting into the cave.
9. promotion agalloch eaglewood as claimed in claim 8 belongs to the method that (Aquilaria), plan agalloch eaglewood genus (Gyrinops) or other inducible plants produce agalloch eaglewood, it is characterized in that, in described entire body Edgeworthia chrysantha technology vaccination ways, on the tree that at least one in the bacterium liquid of gained, bacteria-free filtrate, bacterial strain fermentation liquor concentrate formulation, bacterial strain fermentation liquor filtrate concentrate formulation directly belongs to entire body Edgeworthia chrysantha method input agalloch eaglewood.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340602A (en) * | 2015-09-29 | 2016-02-24 | 中国医学科学院药用植物研究所海南分所 | Dot-like and/or dot-line-like aligawood producing method and application |
| CN109541116A (en) * | 2018-11-23 | 2019-03-29 | 北京三和药业有限公司 | A kind of discrimination method of agalloch eaglewood |
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| CN113647414A (en) * | 2021-07-19 | 2021-11-16 | 广东湛江海洋医药研究院 | Aquilaria plant edgeworthia chrysantha promoter as well as preparation method and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340602A (en) * | 2015-09-29 | 2016-02-24 | 中国医学科学院药用植物研究所海南分所 | Dot-like and/or dot-line-like aligawood producing method and application |
| CN109541116A (en) * | 2018-11-23 | 2019-03-29 | 北京三和药业有限公司 | A kind of discrimination method of agalloch eaglewood |
| CN110982714A (en) * | 2020-01-15 | 2020-04-10 | 北京林业大学 | Scleroderma microrum and application thereof |
| CN111713328A (en) * | 2020-07-07 | 2020-09-29 | 刘志强 | Technology for planting agilawood fungi into daphneceae trees for forming incense |
| CN113647414A (en) * | 2021-07-19 | 2021-11-16 | 广东湛江海洋医药研究院 | Aquilaria plant edgeworthia chrysantha promoter as well as preparation method and application thereof |
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| Publication number | Publication date |
|---|---|
| CN104845892B (en) | 2020-09-29 |
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