CN1745647A - Production of enzyme-enriched protein forage from beer vinasse - Google Patents
Production of enzyme-enriched protein forage from beer vinasse Download PDFInfo
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- CN1745647A CN1745647A CNA2005100447478A CN200510044747A CN1745647A CN 1745647 A CN1745647 A CN 1745647A CN A2005100447478 A CNA2005100447478 A CN A2005100447478A CN 200510044747 A CN200510044747 A CN 200510044747A CN 1745647 A CN1745647 A CN 1745647A
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- grains
- brewex
- enzyme
- mass ratio
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
A process for preparing the enzyme-enriched protein feed from the distiller's grains generated by brewing beer includes such steps as inoculating the rejected yeast mud containing living bacteria (more than one hundred million per ml) to said distiller's grains, stirring, fermenting for 1-2 days, inoculating high-activity Aspergillus niger, fermenting at 20-30 Deg C. for 1-2 days, baking and pulverizing.
Description
The present invention relates to a kind of brewex's grains and produce the method for rich zymoprotein feed, belong to brewex's grains feed processing technology field.
At present brewex's grains sell wet poor or dry, the back of granulating and sell as feedstuff, do not have digestive ferment isoreactivity composition.Existing brewex's grains processing method exists product to be of low nutritive value, to exist more NSP (SNSP) ANFs, palatability is poor, added value is low defective, and the discarded yeast of beer is not fully utilized.Study a kind of brewex's grains and produce rich zymoprotein feed method, be of low nutritive value, exist more ANFs, palatability is poor, added value is low and discarded yeast does not make full use of the defective that solve that prior art exists become the major subjects of art technology tackling key problem.
The purpose of this invention is to provide a kind of brewex's grains and produce the method for rich zymoprotein feed, utilize the fermentation of Aspergillus niger brewex's grains of discarded yeast and high enzyme characteristic alive, make brewex's grains improve nutritive value, reduce ANFs, improve palatability, improve added value, make product become high protein, high enzyme high-quality feed raw material alive, thereby overcome the above-mentioned deficiency of existing in prior technology.
The objective of the invention is to realize: make wet brewex's grains moisture reach 50-80% with following technical scheme, the discarded yeast paste of viable count more than 100,000,000/milliliter is inoculated in the brewex's grains according to mass ratio 1-10%, mix thoroughly, ventilation or stuffiness fermentation is 1-2 days under 15-35 ℃ of environment or natural conditions, suitably drying makes moisture reach 40-60%, inoculate high enzyme aspergillus niger strain alive according to mass ratio 0.1-1% then, 20-30 ℃ of aerobic fementation (expect thick can stuffiness) 1-2 days less than 30cm, through oven dry, pulverize, product moisture content is less than 14%, crude protein content is greater than 35%, acid protease is greater than 1000U/g, NSP enzyme (cellulase, zytase, the dextranase summation) greater than 3000U/g, sediments microscope inspection (blood counting chamber method) thalline content promptly gets the rich zymoprotein feed product of brewex's grains greater than 200,000,000/gram.
The rich zymoprotein feed of brewex's grains production of the present invention method has advantage easy to operate, that technology simple, investment is little, the brewex's grains richness zymoprotein feed of producing with method of the present invention have be of high nutritive value, ANFs is few, good palatability, be rich in the advantage of protease, NSP enzyme.
Below in conjunction with embodiment the present invention is described in further details:
Embodiment 1: wet spent wash is divided into 80%, the discarded viable yeast mud of 300,000,000/milliliter of viable counts is inoculated in the brewex's grains according to mass ratio 10%, mix thoroughly, aerobic fementation is 1.5 days under 20 ℃ of environment, shining cold makes moisture reach 60%, then according to the high enzyme of 0.1% inoculation aspergillus niger strain alive, 30 ℃ of aerobic fementations 2 days, through oven dry, after the pulverizing, moisture 12%, crude protein content 38%, acid protease 2100U/g, the NSP enzyme (wherein, cellulase 2400, zytase 1800, dextranase 1200) 5400U/g, blood counting chamber measure 300,000,000 of thalline/gram.
Embodiment 2: wet brewex's grains moisture is 50%, the discarded yeast paste of 100,000,000/milliliter of viable counts is inoculated in the brewex's grains according to mass ratio 1%, mix thoroughly, aerobic fementation is 2 days under field conditions (factors), drying makes moisture reach 40%, then according to the high enzyme of mass ratio 1% inoculation aspergillus niger strain alive, 20 ℃ of stuffiness were fermented 1 day, through oven dry, pulverize, product moisture content 14%, crude protein content 35%, acid protease 1200U/g, the NSP enzyme (wherein, cellulase 1400, zytase 1000, dextranase 800) 3200U/g, sediments microscope inspection (blood counting chamber method) thalline content is greater than 100,000,000/gram.
Embodiment 3: wet spent wash is divided into 70%, the discarded viable yeast mud of 200,000,000/milliliter of viable counts is inoculated in the brewex's grains according to mass ratio 3%, mix thoroughly, aerobic fementation is 1 day under 30 ℃ of environment, moisture reaches 60%, then according to the high enzyme of 0.3% inoculation aspergillus niger strain alive, 30 ℃ of aerobic fementations 1 day, after oven dry, pulverizing, moisture 13%, crude protein content 39%, acid protease 1600U/g, NSP enzyme are (wherein, cellulase 2000, zytase 1400, dextranase 1000) 4400U/g, blood counting chamber is measured 500,000,000 of thalline/gram.
Claims (1)
1, a kind of brewex's grains are produced the method for rich zymoprotein feed, it is characterized in that: make wet brewex's grains moisture reach 50-80%, the discarded yeast paste of viable count more than 100,000,000/milliliter is inoculated in the brewex's grains according to mass ratio 1-10%, mix thoroughly, ventilation or stuffiness fermentation is 1-2 days under 15-35 ℃ of environment or natural conditions, suitably drying makes moisture reach 40-60%, inoculate high enzyme aspergillus niger strain alive according to mass ratio 0.1-1% then, 20-30 ℃ of aerobic fementation (expect thick can stuffiness) 1-2 days less than 30cm, through oven dry, pulverize, product moisture content is less than 14%, crude protein content is greater than 35%, acid protease is greater than 1000U/g, NSP enzyme (cellulase, zytase, the dextranase summation) greater than 3000U/g, sediments microscope inspection (blood counting chamber method) thalline content is greater than 200,000,000/gram.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CNA2005100447478A CN1745647A (en) | 2005-09-22 | 2005-09-22 | Production of enzyme-enriched protein forage from beer vinasse |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CNA2005100447478A CN1745647A (en) | 2005-09-22 | 2005-09-22 | Production of enzyme-enriched protein forage from beer vinasse |
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CN1745647A true CN1745647A (en) | 2006-03-15 |
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CNA2005100447478A Pending CN1745647A (en) | 2005-09-22 | 2005-09-22 | Production of enzyme-enriched protein forage from beer vinasse |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101779749A (en) * | 2010-03-19 | 2010-07-21 | 北京燕京啤酒股份有限公司 | Production method for adding brewer grain fattening pannage |
CN102433315A (en) * | 2011-11-22 | 2012-05-02 | 青岛科技大学 | Method for preparing cellulose and active carbon from beer grains |
CN102687792A (en) * | 2012-06-01 | 2012-09-26 | 江苏大学 | Feeding microecologic preparation based on beer grains and rice bran meal |
CN109463572A (en) * | 2018-11-27 | 2019-03-15 | 广州科盛生物科技有限公司 | It is a kind of to enhance disease-resistant and immunocompetence feed stuff for pet fish |
-
2005
- 2005-09-22 CN CNA2005100447478A patent/CN1745647A/en active Pending
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101779749A (en) * | 2010-03-19 | 2010-07-21 | 北京燕京啤酒股份有限公司 | Production method for adding brewer grain fattening pannage |
CN101779749B (en) * | 2010-03-19 | 2013-01-16 | 北京燕京啤酒股份有限公司 | Production method for adding brewer grain fattening pig feed |
CN102433315A (en) * | 2011-11-22 | 2012-05-02 | 青岛科技大学 | Method for preparing cellulose and active carbon from beer grains |
CN102687792A (en) * | 2012-06-01 | 2012-09-26 | 江苏大学 | Feeding microecologic preparation based on beer grains and rice bran meal |
CN102687792B (en) * | 2012-06-01 | 2013-11-20 | 江苏大学 | Feeding microecologic preparation based on beer grains and rice bran meal |
CN109463572A (en) * | 2018-11-27 | 2019-03-15 | 广州科盛生物科技有限公司 | It is a kind of to enhance disease-resistant and immunocompetence feed stuff for pet fish |
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Addressee: Li Junxun Document name: Notification that Application Deemed to be Withdrawn |
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