CN109463572A - It is a kind of to enhance disease-resistant and immunocompetence feed stuff for pet fish - Google Patents
It is a kind of to enhance disease-resistant and immunocompetence feed stuff for pet fish Download PDFInfo
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- CN109463572A CN109463572A CN201811427778.5A CN201811427778A CN109463572A CN 109463572 A CN109463572 A CN 109463572A CN 201811427778 A CN201811427778 A CN 201811427778A CN 109463572 A CN109463572 A CN 109463572A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
Enhance disease-resistant and immunocompetence feed stuff for pet fish the invention discloses a kind of, which contains corn flour, fly maggot powder, aspergillus niger yeast S grain powder, microencapsulation powdered oil, gelatin, dregs of beans, compound premix, Ca (H2PO4)2And carboxymethyl cellulose.Functionality fish feed provided by the invention is using corn flour as primary raw material, it is mixed into microencapsulation oil powder and mixed fungus fermentation brewex's grains powder, slow down high-fat brought oxidative stress, improve the intestinal environment of fish, it promotes the abundance of beneficial bacterium bacillus and series bacillus and reduces the abundance of harmful bacteria Pseudoalteromonas and rose stain fungus, the disease-resistant and immunocompetence of fish is enhanced, the survival rate of fish is increased.
Description
Technical field
The present invention relates to aquaculture feeds fields, and in particular to a kind of to improve intestinal health and promote the ornamental of immune function
Fish meal.
Background technique
Ornamental fish is the fish for having bright colors or fancy shapes with ornamental value, with mentioning for people's level of consumption
It rises, ornamental fish has been increasingly becoming the new fashion of household consumption.Feed stuff for pet fish is broadly divided into animal fodder at present, vegetalitas is raised
Material, artifical compound feed three categories, artifical compound feed can effectively abandon many deficiencies of animal plant feedstuff, according to ornamental fish
Demand and its physiology, growth, digestion and absorption feature to various nutriments are manually matched, are arranged in pairs or groups, but multiple currently on the market
The artifical compound feed of mould assembly usually have fat content it is excessively high and cause fish lipid oxidative damage or have it is unfavorable to fish
Additive.
Therefore, it is necessary to improve to existing fish meal, forage protein content is improved, improves the high-fat oxidation to fish
Damage, and improve the immunity of organisms of fish and improve the intestinal flora of ornamental fish to improve the anti-inflammatory of ornamental fish, improve ornamental fish
Survival rate, improve efficiency of feed utilization, promote digestion and absorption to nutriment.
Summary of the invention
The present invention provides the feed stuff for pet fish of a kind of improvement intestinal health and promotion immune function, can improve high-fat to fish
The oxidative damage of class, and improve the immunity of organisms of fish and improve the intestinal flora of ornamental fish to improve the anti-inflammatory of ornamental fish, it mentions
The survival rate of high ornamental fish improves efficiency of feed utilization, promotes the digestion and absorption to nutriment.
The purpose of the present invention is to provide the fish meals and its application of a kind of improvement intestinal health and promotion immune function.
The technical solution used in the present invention is:
One aspect of the present invention provides the fish meal of a kind of improvement intestinal health and promotion immune function, the fish meal
Raw material composition are as follows: according to parts by weight, 30~40 parts of corn flour, 515 parts of fly maggot powder, aspergillus niger yeast S grain powder 10~15
Part, 20~25 parts of microencapsulation powdered oil, 4~6 parts of gelatin, 4~8 parts of dregs of beans, 0.5~1 part of compound premix, Ca
(H2PO4)21.4~1.6 parts and 4~6 parts of carboxymethyl cellulose；
Contain palm oil and linseed oil in the capsule of the microencapsulation powdered oil；
The aspergillus niger yeast S grain powder the preparation method comprises the following steps: the mixed bacteria of aspergillus niger and yeast is inoculated in solid
Carry out fermented and cultured in fermentation medium, tunning through drying, crush to obtain aspergillus niger yeast S grain powder.
Preferably, above-mentioned fish meal is formed by the raw material mixture of following parts by weight: 40 parts of corn flour, 7 parts of fly maggot powder, black
12 parts of aspergillus yeast S grain powder, 25 parts of microencapsulation powdered oil, 6 parts of gelatin, 5 parts of dregs of beans, 0.5 part of compound premix, Ca
(H2PO4)21.5 parts and 5 parts of carboxymethyl cellulose.
Preferably, the mass ratio of the palm oil and linseed oil is 32~35:21~24, the microencapsulation oil powder
End is using casein and dextrin as cyst material.
Preferably, the preparation method of the microencapsulation powdered oil, specifically includes following steps are as follows:
The raw material proportioning of microencapsulation powdered oil are as follows: based on parts by weight, 32~35 parts of palm oil, linseed oil 21~
24 parts, 10~15 parts and 30~35 parts of dextrin, 1.5~2 parts of moisture of casein；
The preparation of microencapsulation powdered oil: casein and dextrin mix and pass through 70~80 DEG C of heating for dissolving shapes
At aqueous phase solution, while palm oil and linseed oil are heated to 70~80 DEG C and are mixed to form oil-phase solution, then by aqueous phase solution
Be mixed to form feed liquid with oil-phase solution, feed liquid after colloid mill is high-pressure homogeneous at 20~40MPa, control inlet air temperature 180~
200 DEG C, 90~97 DEG C of leaving air temp are spray-dried to get microencapsulation powdered oil feed liquid.
Preferably, the mixed bacteria of 15wt%~20wt% is accessed in solid fermentation culture medium.
Preferably, in the mixed bacteria aspergillus niger seed liquor and the volume ratio of yeast starter liquid be 0.8~1.2:0.8~
1.2。
Preferably, the aspergillus niger seed liquor the preparation method comprises the following steps: take aspergillus niger maturation spore, be inoculated in PB Liquid Culture
In base, 27~29 DEG C, 160~200rpm cultivates 22~26h；
Preferably, the yeast starter liquid the preparation method comprises the following steps: by yeast thallus, be inoculated in LB liquid medium, 27
~29 DEG C, 160~200rpm cultivates 5~7h.
Preferably, based on parts by weight, the solid fermentation culture medium is by 85~90 parts of brewex's grains, 4~6 parts of wheat bran and ferment
4~6 parts of female mud compositions.
Preferably, the temperature of the fermented and cultured is 28~30 DEG C, and the time of fermented and cultured is 4~6 days.
Preferably, after the tunning is chilled or heated-air drying to constant weight, aspergillus niger yeast S grain powder is crushed to obtain.
Preferably, the compound premix is commercially available fish compound premix.
Preferably, yeast of the present invention is at least one of brewer's yeast, saccharomyces uvarum and Lu Shi yeast.
Another aspect of the present invention, which provides fish meal described in any of the above embodiments, improves fish intestines Bacterial community in preparation
Fish meal in apply.
Preferably, it is described improve fish intestines Bacterial community include the abundance for promoting bacillus and series bacillus, under
Adjust the abundance of Pseudoalteromonas and rose stain fungus.
Further aspect of the present invention provides fish meal described in any of the above embodiments and slows down high-fat brought oxygen in preparation
Change stress fish meal in apply.
Preferably, it is described slow down it is high-fat brought by oxidative stress include reduce fish body MDA, GSH-PX, TNF-α contains
Amount.
Further aspect of the present invention, which provides fish meal described in any of the above embodiments, reduces enzyme ACP, AKP in body fish body in preparation
And/or it is applied in the active fish meal of LZM.
Preferably, fish meal of the present invention is used for the raising of ornamental fish, and the ornamental fish includes goldfish, fancy carp.
The beneficial effects of the present invention are:
The present invention is prepared with the growth characteristics and appreciation effect of ornamental fish, improves the survival rate of ornamental fish, is improved
The gloss of ornamental fish, slow down it is high-fat brought by oxidative stress, reduce the expression of in-vivo tumour necrosis factor TNF-alpha
With the activity of immune relevant enzyme ACP, AKP and LZM, while improving the structure of intestinal flora, promoted beneficial bacterium bacillus and
The abundance of series bacillus and the abundance for reducing harmful bacteria Pseudoalteromonas and rose stain fungus；Feed of the present invention promotees significantly
Into the survival rate and health degree of ornamental fish, the economic benefit of cultivation ornamental fish can be effectively improved.
Detailed description of the invention
Fig. 1 is survival rate of the different feeds to ornamental fish in pathogenic bacteria, this feed refers to embodiment feed in figure.
Fig. 2 is survival rate of the different feeds to ornamental fish in pathogenic bacteria, this feed refers to embodiment feed in figure.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below.
Embodiment 1
The feed stuff for pet fish (goldfish) for improving intestinal health and promoting immune function of the present embodiment, including following weight parts
Several raw material compositions: 40 parts of corn flour, 7 parts of fly maggot powder, 12 parts of aspergillus niger yeast S grain powder, microencapsulation powdered oil
25 parts of (containing palm oil and linseed oil, using casein and dextrine powder as wall material), 6 parts of gelatin, 5 parts of dregs of beans, compound premix
0.5 part of (for commercially available fish compound premix), Ca (H2PO4)21.5 parts and 5 parts of carboxymethyl cellulose.
Wherein, the preparation method of aspergillus niger yeast S grain powder, specifically includes following steps are as follows:
(1) preparation of solid fermentation culture medium: fermentation substrate is brewex's grains, wheat bran and yeast paste, based on parts by weight, beer
90 parts of vinasse, 5 parts of wheat bran, 5 parts of yeast paste, 121 DEG C of high-temp steam sterilizing 20min.
(2) prepared by the seed liquor of aspergillus niger and saccharomycete: scraping aspergillus niger maturation spore from plate, is inoculated in 100mL
In PB fluid nutrient medium, 28 DEG C, 180rpm is cultivated for 24 hours, obtains aspergillus niger seed liquor；Brewer's yeast thallus is picked from the plate, is connect
Kind is in 100mL LB liquid medium, and 28 DEG C, 180rpm cultivates 6h, obtains brewer's yeast seed liquor.
The formula of the PB fluid nutrient medium are as follows: beef extract, peptone, sodium chloride, agar ratio be 45g:92g:
45g:120g:8000ml, and the 30min that sterilizes in 119 DEG C of environment obtain PB culture medium.
(3) 15wt% the preparation of aspergillus niger yeast S grain powder: is added in the solid fermentation culture medium that step (1) obtains
~20wt% mixed bacteria, wherein the volume ratio of mixed bacteria aspergillus niger seed liquor and brewer's yeast seed liquor is 1:1, in temperature
Fermented and cultured 6 days under the conditions of 28~30 DEG C of degree, aspergillus niger yeast S grain fermentation material, chilled or heated-air drying to perseverance is made
Aspergillus niger yeast S grain powder is made through crushing in weight.
Wherein, the preparation method of microencapsulation powdered oil, specifically includes following steps are as follows:
(1) raw material proportioning of microencapsulation powdered oil are as follows: based on parts by weight, 33 parts of palm oil, 22 parts of linseed oil,
13.5 parts and 29.7 parts of dextrin, 1.8 parts of moisture of casein；
(2) preparation of microencapsulation powdered oil: casein and dextrin mix and 70~80 DEG C of heating of process are molten
Solution forms aqueous phase solution, while palm oil and linseed oil are heated to 70~80 DEG C in oily phase tank and are mixed to form oil-phase solution,
Aqueous phase solution and oil-phase solution are mixed to form feed liquid again, feed liquid is after colloid mill is high-pressure homogeneous at 40MPa, control air inlet temperature
190 DEG C of degree, 97 DEG C of leaving air temp are spray-dried to get microencapsulation powdered oil feed liquid.
Production method: each constituent of the feed is made together and by stirring by above-mentioned weight ratio mixture
It is uniformly mixed, and suitable quantity of water is added and is made into feed group, feed group is put into pelletizer and is granulated, then by particle screen selecting, pack
?.
For the inspection that feed of the present invention improves the survival rate of ornamental fish, with common fish meal group (commercially available) and this feed
100 ornamental fish (goldfish) are fed respectively, and ornamental fish is both exposed to pathogenic bacteria Aeromonas hydrophila, carry out survival rate inspection
It surveys.
At digit rate testing result as shown in Figure 1, ornamental fish is in pathogenic bacteria (thermophilic aqueous vapor list after finding edible feed of the present invention
Born of the same parents bacterium) in survival rate be substantially better than commercially available common fish meal, and be the 291.7% of normal diet in the 10th day survival rate.
In order to further detect feed of the present invention whether have slow down it is high-fat brought by oxidative stress, reduce it is swollen in vivo
The expression of tumor necrosis factor TNF-α, and immunization feedstuff of the present invention is to the activity influence of relevant enzyme ACP, AKP and LZM.It is provided with
It tests below, 15 ornamental fish (goldfish), other feeding conditions is fed respectively with the fish meal of common grease and feed of the present invention
It is all the same, the GSH-PX gene relative expression quantity in serum and liver MDA and liver in the 8th week progress lipid oxidation index
Measurement, the measurement of tumor necrosis factor TNF-alpha expression and the work of immune relevant enzyme ACP, AKP and LZM in liver and spleen
Property measurement.
Find through detection: after feeding feed of the present invention, the content of the serum MDA of ornamental fish reduces 6.7%, liver MDA
Content reduce 33.6%, and the relative expression quantity of GSH-PX has lowered 46.1%；Have also discovered feeding feed of the present invention
Afterwards, TNF-α expression quantity reduces 35.2% and 59.4% respectively in the liver and spleen of ornamental fish,；Simultaneously it has also been found that feeding sheet
After feed, the activity of immune relevant enzyme ACP, AKP and LZM in ornamental fish serum reduce 28.1%, 33.0% and respectively
52.5%.
In order to further detect whether feed of the present invention has the function of improving fish intestines Bacterial community, it is provided with following
Experiment feeds 15 ornamental fish (goldfish) with the fish meal of common grease and this feed respectively, and to two groups that the 8th week obtains
The intestinal contents of fish, and further detect its intestinal flora.
Find through detection: after feeding feed of the present invention, compared to normal diet group, the structure of the intestinal flora of ornamental fish is obtained
Arriving improvement, the relative abundance of beneficial bacterium bacillus and series bacillus improves 256.2% and 162.5% respectively, and
The relative abundance of harmful bacteria Pseudoalteromonas and rose stain fungus has lowered 71.2% and 81.5% respectively.
Embodiment 2
The feed stuff for pet fish for improving intestinal health and promoting immune function of the present embodiment, the original including following portions by weight
Material composition: 40 parts of corn flour, 7 parts of fly maggot powder, 12 parts of aspergillus niger yeast S grain powder, microencapsulation powdered oil (contain palm
Oil and linseed oil, using casein and dextrine powder as wall material) 25 parts, 6 parts of gelatin, 5 parts of dregs of beans, 0.5 part of compound premix, Ca
(H2PO4)21.5 parts and 5 parts of carboxymethyl cellulose.
Wherein, the preparation method of aspergillus niger yeast S grain powder, specifically includes following steps are as follows:
(1) preparation of solid fermentation culture medium: fermentation substrate is brewex's grains, wheat bran and yeast paste, based on parts by weight, beer
90 parts of vinasse, 5 parts of wheat bran, 5 parts of yeast paste, 121 DEG C of high-temp steam sterilizing 20min.
(2) prepared by the seed liquor of aspergillus niger and saccharomycete: scraping aspergillus niger maturation spore from plate, is inoculated in 100mL
In PB fluid nutrient medium, 28 DEG C, 180rpm is cultivated for 24 hours, obtains aspergillus niger seed liquor；Saccharomyces uvarum thallus is picked from the plate,
It is inoculated in 100mL LB liquid medium, 28 DEG C, 180rpm cultivates 6h, obtains saccharomyces uvarum seed liquor.
(3) 15wt% the preparation of aspergillus niger yeast S grain powder: is added in the solid fermentation culture medium that step (1) obtains
~20wt% mixed bacteria, wherein the volume ratio of mixed bacteria aspergillus niger seed liquor and saccharomyces uvarum seed liquor is 1:1,
Aspergillus niger yeast S grain fermentation material is made in fermented and cultured 6 days under the conditions of 28~30 DEG C of temperature, and chilled or heated-air drying is extremely
Aspergillus niger yeast S grain powder is made through crushing in constant weight.
Wherein, the preparation method of microencapsulation powdered oil, specifically includes following steps are as follows:
(1) raw material proportioning of microencapsulation powdered oil are as follows: based on parts by weight, 33 parts of palm oil, 22 parts of linseed oil,
12.5 parts and 30.5 parts of dextrin, 2 parts of moisture of casein；
(2) preparation of microencapsulation powdered oil: casein and dextrin mix and 70~80 DEG C of heating of process are molten
Solution forms aqueous phase solution, while palm oil and linseed oil are heated to 70~80 DEG C in oily phase tank and are mixed to form oil-phase solution,
Aqueous phase solution and oil-phase solution are mixed to form feed liquid again, feed liquid is after colloid mill is high-pressure homogeneous at 40MPa, control air inlet temperature
180 DEG C of degree, 95 DEG C of leaving air temp are spray-dried to get microencapsulation powdered oil feed liquid.
Production method: each constituent of the feed is made together and by stirring by above-mentioned weight ratio mixture
It is uniformly mixed, and suitable quantity of water is added and is made into feed group, feed group is put into pelletizer and is granulated, then by particle screen selecting, pack
?.
For the inspection that feed of the present invention improves the survival rate of ornamental fish, with common fish meal group (commercially available) and this feed
100 ornamental fish (goldfish) are fed respectively, and ornamental fish is both exposed to pathogenic bacteria Aeromonas hydrophila, carry out survival rate inspection
It surveys.
After survival rate testing result is as shown in Fig. 2, find edible feed of the present invention, survival rate of the ornamental fish in pathogenic bacteria
It is substantially better than common fish meal, and is the 287.5% of normal diet in the tenth day survival rate.
In order to further detect feed of the present invention whether have slow down it is high-fat brought by oxidative stress, reduce it is swollen in vivo
The expression of tumor necrosis factor TNF-α, and immunization feedstuff of the present invention is to the activity influence of relevant enzyme ACP, AKP and LZM.It is provided with
It tests below.15 ornamental fish (goldfish) are fed respectively with the fish meal of common grease and this feed, other feeding conditions are homogeneous
Together, and at the 8th week GSH-PX gene relative expression quantity in serum and liver MDA and liver is carried out in lipid oxidation index
The activity of the measurement of tumor necrosis factor TNF-alpha expression and immune relevant enzyme ACP, AKP and LZM in measurement, liver and spleen
Measurement.
Find through detection: after this feed of feeding, the content of the serum MDA of ornamental fish reduces containing for 7.2%, liver MDA
Amount reduces 32.7%, and the relative expression quantity of GSH-PX has lowered 48.3%；After having also discovered this feed of feeding, ornamental fish
Liver and spleen in TNF-α expression quantity reduce 33.2% and 55.8% respectively,；Simultaneously it has also been found that after this feed of feeding, see
The activity of immune relevant enzyme ACP, AKP and LZM in reward fish serum reduce 30.4%, 33.5% and 50.6% respectively.
In order to further detect whether feed of the present invention has the function of improving fish intestines Bacterial community, it is provided with following
Experiment feeds 15 ornamental fish (goldfish) with the fish meal of common grease and this feed respectively, and to two groups that the 8th week obtains
The intestinal contents of fish, and further detect its intestinal flora.
Find through detection: after this feed of feeding, compared to normal diet group, the structure of the intestinal flora of ornamental fish is obtained
Improve, the relative abundance of beneficial bacterium bacillus and series bacillus improves 248.6% and 148.2% respectively, and harmful
The relative abundance of bacterium Pseudoalteromonas and rose stain fungus has lowered 74.1% and 79.4% respectively.
In conclusion the present invention is prepared with the growth characteristics and appreciation effect of ornamental fish, make the survival rate of ornamental fish
It improves, improves the gloss of ornamental fish, slow down high-fat brought oxidative stress, reduce in-vivo tumour necrosin
The activity of the expression of TNF-α and immune relevant enzyme ACP, AKP and LZM, while the structure of intestinal flora is improved, promote beneficial bacterium
The abundance of bacillus and series bacillus and the abundance for reducing harmful bacteria Pseudoalteromonas and rose stain fungus；The present invention
Nutritional ingredient is uniform, greatly promotes the survival rate and health degree of ornamental fish, can effectively improve the economic benefit of cultivation ornamental fish.
The present invention provides the feed stuff for pet fish of a kind of improvement intestinal health and promotion immune function, and it is high-fat right to improve
The oxidative damage of fish, and improve the immunity of organisms of fish and improve the intestinal flora of ornamental fish and improve the anti-inflammatory of ornamental fish,
The survival rate of ornamental fish is improved, efficiency of feed utilization is improved, promotes the digestion and absorption to nutriment.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of fish meal for improving intestinal health and promoting immune function, it is characterised in that: the raw material of the fish meal forms
Are as follows: according to parts by weight, 30~40 parts of corn flour, 5 15 parts of fly maggot powder, 10~15 parts of aspergillus niger yeast S grain powder, microcapsules
Change 20~25 parts of powdered oil, 4~6 parts of gelatin, 4~8 parts of dregs of beans, 0.5~1 part of compound premix, Ca (H2PO4)21.4~
1.6 parts and 4~6 parts of carboxymethyl cellulose；
Contain palm oil and linseed oil in the capsule of the microencapsulation powdered oil；
The aspergillus niger yeast S grain powder the preparation method comprises the following steps: the mixed bacteria of aspergillus niger and yeast is inoculated in solid fermentation
Carry out fermented and cultured in culture medium, tunning through drying, crush to obtain aspergillus niger yeast S grain powder.
2. a kind of fish meal for improving intestinal health and promoting immune function according to claim 1, it is characterised in that: institute
The mass ratio for stating palm oil and linseed oil is 32~35:21~24, and the microencapsulation powdered oil is with casein and dextrin
For cyst material.
3. a kind of fish meal for improving intestinal health and promoting immune function according to claim 1, it is characterised in that: institute
The preparation method for stating microencapsulation powdered oil, specifically includes following steps are as follows:
The raw material proportioning of microencapsulation powdered oil are as follows: based on parts by weight, 32~35 parts of palm oil, linseed oil 21~24
Part, 10~15 parts and 30~35 parts of dextrin, 1.5~2 parts of moisture of casein；
The preparation of microencapsulation powdered oil: casein and dextrin mix and form water by 70~80 DEG C of heating for dissolving
Phase solution, while palm oil and linseed oil are heated to 70~80 DEG C and are mixed to form oil-phase solution, then by aqueous phase solution and oil
Phase solution is mixed to form feed liquid, and feed liquid controls inlet air temperature 180~200 after colloid mill is high-pressure homogeneous at 20~40MPa
DEG C, 90~97 DEG C of leaving air temp are spray-dried to get microencapsulation powdered oil feed liquid.
4. a kind of fish meal for improving intestinal health and promoting immune function according to claim 1, it is characterised in that: Gu
The mixed bacteria of 15wt%~20wt% is accessed in body fermentation medium；Aspergillus niger seed liquor and yeast kind in the mixed bacteria
The volume ratio of sub- liquid is 0.8~1.2:0.8~1.2.
5. a kind of fish meal for improving intestinal health and promoting immune function according to claim 1, it is characterised in that: institute
Solid fermentation culture medium is stated to be made of 85~90 parts of brewex's grains, 4~6 parts of wheat bran and 4~6 parts of yeast paste based on parts by weight；Institute
The temperature for stating fermented and cultured is 28~30 DEG C, and the time of fermented and cultured is 4~6 days.
6. the described in any item fish meals of Claims 1 to 5 are applied in the fish meal that preparation improves fish intestines Bacterial community.
7. application according to claim 6, which is characterized in that the improvement fish intestines Bacterial community includes promoting gemma
The abundance of bacillus and series bacillus lowers the abundance of Pseudoalteromonas and rose stain fungus.
8. the fish meal that the described in any item fish meals of Claims 1 to 5 slow down high-fat brought oxidative stress in preparation
Middle application.
9. application according to claim 8, which is characterized in that described to slow down high-fat brought oxidative stress including dropping
The content of low fish body MDA, GSH-PX, TNF-α.
10. the described in any item fish meals of Claims 1 to 5 reduce enzyme ACP, AKP and/or LZM activity in body fish body in preparation
Fish meal in apply.
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| CN201811427778.5A CN109463572A (en) | 2018-11-27 | 2018-11-27 | It is a kind of to enhance disease-resistant and immunocompetence feed stuff for pet fish |
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Application publication date: 20190315 |