CN1724036A - Medicine for improving internal secreting melatonin, and application of its related herb medicine - Google Patents
Medicine for improving internal secreting melatonin, and application of its related herb medicine Download PDFInfo
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- CN1724036A CN1724036A CN 200410028154 CN200410028154A CN1724036A CN 1724036 A CN1724036 A CN 1724036A CN 200410028154 CN200410028154 CN 200410028154 CN 200410028154 A CN200410028154 A CN 200410028154A CN 1724036 A CN1724036 A CN 1724036A
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Abstract
An orally taken Chinese medicine for promoting the secretion of melatonin in human body and improving the function of epiphyses is prepared from 6 Chinese-medicinal materials including white peony root, Chinese angelica root, Chuanxiong rhizome, tuckahoe, etc through grinding to obtain powder. It can be mixed with Western medicine and other Chinese medicines or can be use to prepare health-care food.
Description
[technical field]
The present invention relates to a kind of medicine that promotes body endocrine melatonin, and the application of DANGGUI SHAOYAO SAN in preparation promotion body endocrine melatonin medicine.
[background technology]
Melatonin (MT) separated extraction in 1958 by people such as Lerner, and it mainly is by the excretory a kind of material of pinus.The MT that studies show that in recent years has effect widely to body.1. free radical resisting detrimental effect; 2. immunoregulation effect; 3. adjust the function of biological clock; 4. regulate the reproduction reaction; 5. Shui Mian regulating action; 6. the regulating action of pain sensation process; 7. stress, the effects such as adjusting of auxiliary aspect such as anticancer.Increase along with the age, pinus secretion MT reduces, the secretion rhythm and pace of moving things disappears, this can have influence on the above-mentioned effect of MT naturally, and this may be one of mechanism that causes body aging, therefore, researcher is used for antidotal effect research to MT, find that exogenous MT has the free radical damage of obvious antagonism aging, improve the immunologic function of senile organism, improve study, memory ability and the motor capacity etc. of old Mus.Human is also arranged in the treatment alzheimer disease, discovery can delay the further deterioration of relevant symptom.Therefore be considered to ideal defying age material.Because pineal rhythmicity secretion MT, the circadian rhythm of MT in the blood " height at day at low night " is the basis of keeping its physiological action.Though exogenous MT can make the MT content in the blood increase, and is difficult to simulate the increase and decrease gradually of MT Circadian rhythm in the body blood, makes exogenous MT be difficult to better play a role.Therefore can improve endogenous MT secretion, keep the medicine of the pineal rhythmicity secretion of old body MT, may be the old and feeble good medicine of control.
DANGGUI SHAOYAO SAN (DSS) comes from " Medical Treasures of the Golden Chamber ", cures mainly the ancient prescription commonly used of gynaecopathia, and the function of nourshing blood and promoting blood circulation, removing dampness, diuretic is arranged, and the past, existing clinical and experimental data in a large number showed that DSS was effective prescription of treatment various gynecological diseases.Studies show that DSS has extensive pharmacological action.1. free radical resisting detrimental effect; 2. enhancing human body immunity function; 3. promote the function of hypothalamic-pituitary-ovarian axis; 4. improve old people's sleep; 5. assist antitumaous effect etc.The clear treatment alzheimer disease of using it for first of Japan scholar water island a surname at the end of the eighties, obtain preliminary curative effect, there is the people to find that respectively this can be to stimulate the synthetic of maincenter acetylcholine and receptor subsequently, improve the learning and memory in rats ability, the continuous many data that the DSS anti-aging effects occurred of domestic afterwards foreign minister.Studies show that this medicine can improve the senile learning and memory Mus ability of learning and memory that goes down, improve the motor capacity of old Mus, promote the function of old Mus central cholinergic system, effects such as defying age free radical damage, be considered to a kind of wide spectrum defying age prescription, and DSS is to the effect of body and the effect fairly similar of MT, as anti-radical action, immunoregulation effect, reproduction regulating action etc.; Yet whether DSS has the endogenous of raising MT secretion, reaches anti-aging effects, does not see that up to the present relevant DSS regulates the excretory report of endogenous MT.
[summary of the invention]
The objective of the invention is in order to overcome the shortcoming that exogenous MT is difficult to better play a role, search out the medicine that improves endogenous MT secretion, keeps the pineal rhythmicity secretion of old body MT.The application of DANGGUI SHAOYAO SAN on the excretory medicine of preparation raising endogenous MT is provided.
The present invention is achieved in that the former side of DSS is for " Medical Treasures of the Golden Chamber " (married woman is pregnent in the bosom, and abdominal pain is when GUISHAO is loose the master's).Three liang of Radix Angelicae Sinensis, one jin of Radix Paeoniae (does six liang), four liang in Poria, four liang of the Rhizoma Atractylodis Macrocephalaes, half jin of Rhizoma Alismatis, half jin of Rhizoma Chuanxiong (doing three liang), last Six-element pestle is got tool for weighing medicine in ancient China for diffusing, wine and, taking medicine three times daily.95 editions contained DSS prescriptions of pharmacopeia are: the Radix Paeoniae Alba, each 9 gram of Radix Angelicae Sinensis, Rhizoma Chuanxiong 6 grams, the Rhizoma Atractylodis Macrocephalae, Rhizoma Alismatis, each 12 gram of Poria.Making up a prescription according to 95 editions pharmacopeia prescriptions adds the 900ml tap water, and slow fire 2 hours, simmer down to 300ml are DSS decocting liquid.With the same preparation process of Chinese medicine decocting liquid phase.
Medicine of the present invention comprises: Radix Paeoniae Alba 9-30 part is heavy, and Radix Angelicae Sinensis 9-20 part is heavy, and Rhizoma Chuanxiong 6-10 part is heavy, and Rhizoma Atractylodis Macrocephalae 8-15 part is heavy, and Rhizoma Alismatis 8-15 part is heavy, and Poria 8-15 part is heavy.
Be good wherein: 9 parts heavy of the Radix Paeoniae Albas, 9 parts heavy of Radix Angelicae Sinensis, 6 parts heavy of Rhizoma Chuanxiongs, 12 parts heavy of the Rhizoma Atractylodis Macrocephalaes, 12 parts heavy of Rhizoma Alismatis, 12 parts of weights of Poria with following proportioning.
Show that through pinus function and the experiment of blood melatonin levels DANGGUI SHAOYAO SAN comprises following aspect to old Mus effect: improve the blood melatonin levels 1. to old Mus.2. improve the pinus function.Therefore DANGGUI SHAOYAO SAN is to improve endogenous MT secretion, keeps the pineal rhythmicity secretion of old body MT ideal medicament.
DANGGUI SHAOYAO SAN of the present invention is prepared into and improves the excretory medicine of endogenous MT, because DANGGUI SHAOYAO SAN is a Chinese medicinal formulae, tradition is by the oral therapeutical effect that reaches, and can make peroral dosage form, comprises tablet, granule, capsule, oral liquid etc.; Also can make compound Chinese medicinal preparation or compound Western medicine preparation; Also can add in the food ediblely, or be added in the food edible as health food.
DANGGUI SHAOYAO SAN of the present invention also can be made compound Chinese medicinal preparation or compound Western medicine preparation with present antidotal medicine, makes at anti-aging effects better.
[description of drawings]
Fig. 1: the DSS and the side of tearing open are to the influence of senile rat pinus β-AR content.
Fig. 2: clothes sDSS and the side of tearing open senile rat PC liquid are to the lymphopoietic influence of T.
[specific embodiment]
The following example only is used to illustrate the present invention, and protection scope of the present invention is not had any restriction.
Embodiment 1.
The preparation of DANGGUI SHAOYAO SAN decocting liquid: get the Radix Paeoniae Alba, each 9 gram of Radix Angelicae Sinensis by prescription, Rhizoma Chuanxiong 6 grams, the Rhizoma Atractylodis Macrocephalae, Rhizoma Alismatis, each 12 gram of Poria add the 900ml tap water, slow fire 2 hours, simmer down to 300ml gets final product.Promptly with the same preparation process of Chinese medicine decocting liquid phase.
Embodiment 2.
The preparation of DANGGUI SHAOYAO SAN decocting liquid: get the Radix Paeoniae Alba 30 grams, Radix Angelicae Sinensis 20 grams by prescription, Rhizoma Chuanxiong 10 grams, the Rhizoma Atractylodis Macrocephalae, Rhizoma Alismatis, each 15 gram of Poria add the 900ml tap water, slow fire 2 hours, simmer down to 300ml gets final product.Promptly with the same preparation process of Chinese medicine decocting liquid phase.
Embodiment 3.
The preparation of DANGGUI SHAOYAO SAN decocting liquid: get the Radix Paeoniae Alba 20 grams, Radix Angelicae Sinensis 15 grams by prescription, Rhizoma Chuanxiong 8 grams, the Rhizoma Atractylodis Macrocephalae, Rhizoma Alismatis, each 8 gram of Poria add the 900ml tap water, slow fire 2 hours, simmer down to 300ml gets final product.Promptly with the same preparation process of Chinese medicine decocting liquid phase.
Embodiment 4
DANGGUI SHAOYAO SAN adds flavor side's powder: get each 9 gram of the Radix Paeoniae Alba, Radix Angelicae Sinensis by prescription, and Rhizoma Chuanxiong 6 grams, the Rhizoma Atractylodis Macrocephalae, Rhizoma Alismatis, each 12 gram of Poria are ground into powder Like Fine end and are DANGGUI SHAOYAO SAN and add flavor side's powder after all crude drugs are mixed.
Embodiment 5
The DANGGUI SHAOYAO SAN capsule: get DANGGUI SHAOYAO SAN powder, packing in capsule filler promptly gets capsule.
Embodiment 6
DANGGUI SHAOYAO SAN is improved the pinus mechanism of the ability of learning and memory of old Mus
One. material and method
1, animal and grouping
Select 32 of 18-20 monthly age SD rats for use, animal is divided into four groups at random: go pinus feedwater group, go pinus administration group, sham-operation feedwater group, sham-operation administration group, 8 every group.
2, animal model
Go the pinus group to extract pinus, sham operated rats is not extractd pinus.After the operation, the feedwater group is irritated stomach cold water 3ml every day, and the administration group is irritated stomach DANGGUI SHAOYAO SAN water decoction 3ml (1g crude drug/1ml), irritated stomach altogether 21 days every day.
3, water maze test
Postoperative capable Morris water maze test in the 17th day.Tested altogether 5 days, and divided the upper and lower noon twice every day, write down respectively by four quadrant entry to the time of finding platform, i.e. escape latency at every turn.If can not find platform in 120 seconds, rat is drawn upper mounting plate, escape latency is designated as 120 seconds.Remove platform the 5th day afternoon, the swimming track of record rat after by a certain quadrant entry, and the ring number of times is worn in numeration, calculates the swimming of T quadrant apart from percentage ratio.
Two. the result
Each is organized average escape latency and is respectively: went pinus feedwater group 60.65 ± 18.91 seconds, and removed pinus administration group 47.26 soil 20.12 seconds, sham-operation feedwater group 48.43 ± 22.20 seconds, sham-operation administration group 49.33 ± 22.13 seconds.Compare with going pinus feedwater group, go pinus administration group, sham-operation feedwater group, the equal significance of sham-operation administration group average escape latency to reduce (P＜0.05), then there was no significant difference between three groups.
Each group wears the ring number of times and the swimming of T quadrant sees Table 1 apart from percentage ratio.
Each group of table 1 on average wears the ring number of times and the T quadrant is swum apart from percentage ratio
| On average wear the ring number of times | The swimming of T quadrant is apart from percentage ratio (%) | |
| Go pinus feedwater group to go pinus administration group sham-operation feedwater group sham-operation administration group | 1.7±0.33 2.5±0.34 2.8±0.39 3.5±0.43 | 30.52±7.13 35.10±10.53 30.83±7.12 41.01±5.55 |
By table as seen: compare with going pinus feedwater group, its excess-three group is worn ring number of times significance and is increased (P＜0.05); Compare with sham-operation administration group, its excess-three group is worn ring number of times significance and is reduced (P＜0.05).Compare with going pinus feedwater group, only the swimming of sham-operation administration group T quadrant increases (P＜0.05) apart from the percentage ratio significance.
Embodiment 7
The DANGGUI SHAOYAO SAN and the side of tearing open are to the influence of pinus β-AR content and function
One, material and method
1. laboratory animal and grouping
Laboratory animal is selected one-level SD rat for use, and male and female have concurrently, and at 18～20 months monthly ages, animal is raised in cages, day alternates with night environment (being provided by Zhongshan Medical Univ.'s Experimental Animal Center).Animal is respectively applied for the research of drug effect and dose-effect relationship, it is the full side of young group, aged control, DSS group, the monarch and his subjects' group, 5 groups of adjuvant drug group that the rat of drug efficacy study is divided into, 24 senile rats of every group, wherein every group is used to measure 16 of pinus β-AR density experiment, is used to measure the pinus culture fluid to 8 of splenocyte ConA induction of lymphocyte multiplication capacity experiments.Be divided into 0.625ml group, 1.25ml group, 2.5ml group, 5ml group and 10ml again and organize dividing into groups according to drug efficacy study of dose-effect relationship research, and every group is 5 senile rats respectively.
2.DSS preparation of decocting liquid and medication
Press full side, monarch, ministerial drug, the adjuvant drug of DSS and fry in shallow oil, for the first time 2h with 8 times of water, 6 times of moisture, 1.5h for the second time is concentrated into and contains 1g crude drug/ML, gavages corresponding Chinese medicine decocting liquid 5ml respectively according to grouping, aged control gavages distilled water 5ml, and young group is not done any processing, continuous 3 weeks.The rat experiment of dose-effect relationship research gives 10ml, 5ml, 2.5ml, 1.25ml, the full side of 0.625ML corresponding D SS and monarch-minister drug decocting liquid respectively, is that 10ML divides secondary to gavage with distilled water diluting, continuous 3 weeks.
3. draw materials
Animal opens cranium and separates pinus in 1% pentobarbital sodium (40mg/kg body weight, lumbar injection) anesthesia down.
Draw materials the time: each group is divided into the Mus of drawing materials night, 0 o'clock at dead of night～1 o'clock draw materials (16); The Mus of drawing materials daytime was drawn materials (8) in the afternoon in 5～6 o'clock, and each group rat of dose-effect relationship research was all drawn materials in 0 o'clock at dead of night～1 o'clock.
4. measure and respectively organize pinus β-AR content
Every group of rat (each 8 each the group rat that only reaches dose-effect relationship research of Mus of drawing materials on draw materials night Mus and daytime) pinus adding PBS buffer, homogenate, directly measure whole pineal β-AR content, measure pinus β-AR content with radioligand receptor binding assay method.
5. measure and respectively organize of the influence of rat pinus culture fluid splenocyte ConA induction of lymphocyte multiplication capacity
5.1 pinus culture supernatant (PC liquid) preparation
Each group RPMI1640 culture fluid (0.5ml/ pipe) that Mus (8/group) pinus puts into 10% calf serum that contains the 1mmol tryptophan of drawing materials night is put 37 ℃ of 5%CO
2Every pipe adds 0.25ml isoproterenol (final concentration 10 μ mol/L) after cultivating 20min in the incubator, continues to cultivate 60 minutes, and cultivation finishes the back and collects supernatant, i.e. PC liquid.
5.2 splenocyte separates and cultivates
With young Mus (two monthly ages) spleen separating Morr. cell, with containing 10% calf serum RPMI1640 culture fluid diluted for use, splenocyte is moved in the culture plate in 96 holes, every hole adds 1 * 10
6Splenocyte, it is 1.25 μ g ConA that simultaneously every hole adds final concentration, adds the pinus culture fluid according to different experiments, adds RPMI1640 culture fluid to the 100 μ l that contains 10% calf serum, CO
2Incubator is cultivated three days (37 ℃ of 5%CO
2).
5.3 obey the DSS and the side of tearing open P of Rats C liquid to cultivating the experiment application of sample of the lymphocytic influence of T
Youth's group, aged control, full side group, the monarch and his subjects' group, adjuvant drug group PC liquid and MT are added each hole respectively, and final concentration is 5% (the MT final concentration is 5 μ g).Simultaneously every hole adds propranolol (final concentration is 10 μ moL).
Senile rat PC liquid is to the experiment application of sample of the lymphopoietic influence of T 5.4 variable concentrations takes traditional Chinese medicine
Full side's group, the monarch and his subjects of variable concentrations are organized each hole of culture plate that PC liquid adds 96 holes, and pinus culture fluid concentration is respectively 10%, 5%, 2.5%, 1.25%, 0.625%, and simultaneously every hole adds propranolol 10 μ mol.
5.5 tetrazolium salts colorimetry (mtt assay)
Every hole adds 50 μ g MTT reaction h behind the spleen cell cultures 72h, form purple crystal, liquid in the sucking-off hole, add 100ml (0.8mol/L) hydrochloric acid aqueous isopropanol, blow and beat repeatedly to crystallization with eight passage adjustable pipettes and to dissolve fully, (wavelength 570nm) measures optical density (OD) value on microplate reader.
Two, result
1, the DSS and the side of tearing open are to the influence of senile rat pinus β-AR content
5 groups of senile rats and young group were put to death (Mus on daytime) respectively in the afternoon at 6 o'clock and are drawn materials and put to death (Mus at night) pinus β-AR content (fmol/ pinus) when drawing materials 1 o'clock late into the night and change as Fig. 1.
The result shows, young group rat pinus β-AR content, and be starkly lower than daytime night, and difference has highly significant meaning (P＜0.01), and the difference between aged control daytime and night disappears.Pinus β-AR the content of taking medicine back full side group daytime and night all obviously raises than aged control, and difference has highly significant meaning (P＜0.01), difference not obvious (P＞0.05) between daytime and night.Difference not obvious (P＞0.05) between the β-AR content of the monarch and his subjects' group and adjuvant drug group and aged control.
2, with the influence of dosage DSS to senile rat pinus β-AR content
Respectively with 0.625,1.25,2.5,5,10ml Chinese medicine decocting liquid gavages senile rat after 3 weeks, full side group, the monarch and his subjects' group and adjuvant drug group senile rat pinus β-AR content (fmol/ pinus) change as table 2.
Table 2 various dose DSS is to the influence of senile rat pinus β-AR content
| The full side of DSS0.625ml group 23.1 ± 2.11 the monarch and his subjects organize 18.9 ± 1.77 adjuvant drug groups 22.6 ± 2.45 | DSS1.25ml 23.1±1.98 21.4±2.35 20.7±1.65 | DSS2.5ml 28.2±2.45 ※ 22.7±1.99 21.5±3.88 | DSS 5ml 30.8±3.16 ※ 19.1±2.85 22.3±2.15 | DSS10ml 22.1±2.58 19.9±1.77 20.5±3.07 |
※ and DSS0.625ml group compare P＜0.01
The result shows, rat pinus β-AR content was obviously more than other each dosage group when full side group dosage was 2.5ml and 5ml, demonstration is along with the increase that gavages DSS dosage, full side group rat pinus β-AR content obviously increases, descend but organize β-AR content to 10ml, the monarch and his subjects' group and adjuvant drug group pinus β-AR content change not obvious between each dosage group.
3, take medicine P of Rats C liquid to cultivating the influence of T lymphopoiesis (OD value)
Clothes DSS and the side's of tearing open senile rat PC liquid are induced OD value such as Fig. 2 of the cultivation of propagation to splenocyte ConA.
The result shows the OD value of the full side of DSS group, the monarch and his subjects' group and MT group apparently higher than matched group, and difference has highly significant meaning (P＜0.01).The rising effect of full side group is bigger than the monarch and his subjects' group, meaning that there was a significant difference (P＜0.05).
4, variable concentrations clothes DSS and the side of tearing open senile rat PC liquid are to cultivating the lymphopoietic influence of T (OD value)
The senile rat PC liquid of full side of variable concentrations clothes DSS and DSS the monarch and his subjects, adjuvant drug is induced the MTTOD value such as the table 3 of enrichment culture to splenocyte ConA.
Table 3 variable concentrations PC liquid is to cultivating the lymphopoietic influence of T
| Full side group the monarch and his subjects organize the adjuvant drug group | 0.625％ 0.399±0.035 0.366±0.021 0.361±0.031 | 1.25ml％ 0.451±0.043 0.451±0.029 0.371±0.027 | 2.5％ 0.545±0.033 ※ 0.478±0.011 0.375±0.044 | 5％ 0.603±0.061 ※ 0.511±0.037 ※ 0.377±0.025 | 10％ 0.553±0.028 ※ 0.583±0.037 ※ 0.365±0.026 |
※ and 0.625% group of comparison, P＜0.01
The result shows, the cultivation T lymphopoiesis OD value that increases the full side of DSS and DSS the monarch and his subjects' group along with PC liquid obviously increases, full side organize PC liquid concentration be 2.5%, 5%, 10% and the monarch and his subjects to organize be that 5%, 10% rat pinus β-AR content is obviously more than 0.625% dosage group (P＜0.01).Each PC liquid concentration group difference of the cultivation T lymphopoiesis OD value of adjuvant drug group is not obvious.
Embodiment 8
DANGGUI SHAOYAO SAN improves the blood melatonin levels of old Mus
One. material and method
1. animal and grouping
Select 32 of 18-20 monthly age SD rats for use, male and female are regardless of.Animal is divided into four groups at random: go pinus feedwater group, go pinus administration group, sham-operation feedwater group, sham-operation administration group, 8 every group.
2. animal model
The pinus art is gone in execution.Be fixed in position finder behind the rat anesthesia, chorista exposes lambdoid suture; Open skull (about 5mm*5mm) in this seam intersection, remove the bone lobe; 2 difference ligation superior sagittal sinus are cut off superior sagittal sinus from point-to-point transmission in front and back; Free and caudal ward opens superior sagittal sinus, exposes the pinus below the torcular and extracts.Sham operated rats is not extractd pinus.After the operation, the feedwater group is irritated stomach cold water 3ml every day, and the administration group is irritated stomach DANGGUI SHAOYAO SAN water decoction 3ml (1g crude drug/1ml), irritated stomach altogether 21 days every day.
3. the blood melatonin is measured
Irritate stomach after 21 days, irritate stomach the last time and respectively organize rat after 1 hour and got blood at night at 11 o'clock, measure the blood melatonin with the method for radioimmunity
Two, result
Each is organized the average blood melatonin and is respectively: remove pinus feedwater group 8.41 ± 6.2pg/ml, remove pinus administration group 16.73 ± 12.1pg/ml, sham-operation feedwater group 9.68 ± 9.21pg/ml, sham-operation administration group 32.02 ± 21.15pg/ml.With go pinus feedwater group, go pinus administration group, sham-operation feedwater group is compared, sham-operation administration group average blood melatonin levels significance increases (P＜0.01), and there was no significant difference between first three groups.
Claims (7)
1, a kind of medicine that promotes body endocrine melatonin comprises: Radix Paeoniae Alba 9-30 part is heavy, and Radix Angelicae Sinensis 9-20 part is heavy, and Rhizoma Chuanxiong 6-10 part is heavy, and Rhizoma Atractylodis Macrocephalae 8-15 part is heavy, and Rhizoma Alismatis 8-15 part is heavy, and Poria 8-15 part is heavy.
2,, it is characterized in that 9 parts heavy of the Radix Paeoniae Albas, 9 parts heavy of Radix Angelicae Sinensis, 6 parts heavy of Rhizoma Chuanxiongs, 12 parts heavy of the Rhizoma Atractylodis Macrocephalaes, 12 parts heavy of Rhizoma Alismatis, 12 parts of weights of Poria according to the described medicine of claim 1.
3, according to the described medicine of claim 1, it is characterized in that adopting peroral dosage form.
4,, it is characterized in that making compound preparation with Western medicine according to the described medicine of claim 1.
5,, it is characterized in that making compound preparation with Chinese medicine extract according to the described medicine of claim 1.
6,, it is characterized in that being added into and make health food in the food according to the described medicine of claim 1.
7, the Radix Paeoniae Alba, Radix Angelicae Sinensis, Rhizoma Chuanxiong, the Rhizoma Atractylodis Macrocephalae, Rhizoma Alismatis, the application of Poria in the medicine of preparation promotion body endocrine melatonin.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101574409B (en) * | 2009-06-11 | 2011-04-20 | 成都中医药大学 | Method of using bitter cold traditional Chinese medicine to construct animal modes with continuous low level of serum melatonin |
| CN101167901B (en) * | 2006-10-26 | 2012-11-07 | 中国人民解放军军事医学科学院毒物药物研究所 | Chinese angelica and peony formula extraction, preparation and medical use thereof |
| CN104547553A (en) * | 2015-01-23 | 2015-04-29 | 葛汝丽 | Traditional Chinese medicine for treating nervous endocrine disorder |
| CN116920030A (en) * | 2023-05-16 | 2023-10-24 | 海南医学院第二附属医院 | Traditional Chinese medicine composition for treating diabetic peripheral neuropathy qi deficiency and blood stasis |
-
2004
- 2004-07-20 CN CN 200410028154 patent/CN1724036A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167901B (en) * | 2006-10-26 | 2012-11-07 | 中国人民解放军军事医学科学院毒物药物研究所 | Chinese angelica and peony formula extraction, preparation and medical use thereof |
| CN101574409B (en) * | 2009-06-11 | 2011-04-20 | 成都中医药大学 | Method of using bitter cold traditional Chinese medicine to construct animal modes with continuous low level of serum melatonin |
| CN104547553A (en) * | 2015-01-23 | 2015-04-29 | 葛汝丽 | Traditional Chinese medicine for treating nervous endocrine disorder |
| CN116920030A (en) * | 2023-05-16 | 2023-10-24 | 海南医学院第二附属医院 | Traditional Chinese medicine composition for treating diabetic peripheral neuropathy qi deficiency and blood stasis |
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