CN101574409B - Method of using bitter cold traditional Chinese medicine to construct animal modes with continuous low level of serum melatonin - Google Patents
Method of using bitter cold traditional Chinese medicine to construct animal modes with continuous low level of serum melatonin Download PDFInfo
- Publication number
- CN101574409B CN101574409B CN2009103031706A CN200910303170A CN101574409B CN 101574409 B CN101574409 B CN 101574409B CN 2009103031706 A CN2009103031706 A CN 2009103031706A CN 200910303170 A CN200910303170 A CN 200910303170A CN 101574409 B CN101574409 B CN 101574409B
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- bitter cold
- low level
- melatonin
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to the technical field of experimental animals, aiming at solving the technical problem of constructing animal modes with continuous low level of serum melatonin. The technical solution of the invention is to use infusodecoction of bitter cold traditional Chinese medicine such as rhubarb for animals every day, with the dosage of 0.2-2.0ml per 10g for 5-15 days continuously. The invention has simple step, can construct animal modes with continuous low level of serum melatonin successfully and repeatedly, thereby having favorable application prospect.
Description
Technical field
The invention belongs to the animal model field, be specifically related to use the construction method of bitter cold Chinese medicine animal modes with continuous low level of serum melatonin.
Background technology
Melatonin (melatonin, be called for short MT) be that the dermatological expert Lerner of Yale University in 1958 is first from the isolated a kind of indoles hormone of the pinus of cattle, usually morning the body intensive amount the highest, following three distinguishing features are arranged: (1) MT is the variation of circadian rhythm, seasonal rhythm in haemoconcentration, and follow the growth at age, the circadian rhythm amplitude of its various physiological functions diminishes, and the phase migration of cyclic activity and asynchronization phenomenon increase; (2) human infantile period MT at night level is the highest, and average MT level descends 80% from child to the adolescence, maintains this level later on, further descends to 50 years old front and back MT level; (3) the residing inside and outside environment of body changes can influence the MT haemoconcentration of body own significantly, and body MT receptor mrna level then is subjected to the influence of illumination and body MT concentration.The MT of human body is mainly derived from pineal secretion, as a kind of modulability hormone, MT almost directly or indirectly influences the secretion of other all hormones, thereby having physiology and a pharmacologically active widely, it is round the clock, seasonal rhythm and immunity of organism, nerve, endocrine and reproductive system etc. have close ties.
Raising along with the Modern Analytical Instrument technology, especially the utilization of modern biotechnology, as utilize integrated enzyme reaction to detect MT content, and make the detection of MT in serum and dynamic monitoring become possibility, also make people that the relation between MT and the human diseases has been had more understanding.Clinical studies show: the body melatonin levels reduces not only relevant with elderly patients insomnia, high cancer morbidity, and be specifically related to many human major diseases, as malignant tumor, presenile dementia (Alzheimer Disease, AD), cardiovascular disease etc.Development along with modern medicine and molecular biotechnology, more information under disease or related pathologies physiological condition provides new opportunity for clinical prevention and treatment about MT and MT receptor thereof, and wherein the achievement of attracting attention most is exactly the i.e. birth that has the antidepressants of brand-new mechanism of action of MT receptor stimulating agent agomelatine.
The main animal model that reduces as the body melatonin levels has at present: 1, removing the pinus animal model, is the main secretory of MT according to pinus promptly, removes the level that pinus promptly can influence MT in the animal body body in the animal infancy.2, the animal model pounded out of MT acceptor gene; 3, continuous light model, process promptly synthetic according to the mammal pinealocyte and secretion MT has tangible circadian rhythm, mainly shows as the synthetic and secretion increase of dark phase time MT, and the synthetic and secretion of illumination phase time MT reduces.Though all be the influence of MT level in focusing on body in above-mentioned model, but in first kind model, in view of containing, pinus comprises MT, serotonin (5-HT, the synthetic in vivo precursor substance of MT), nearly 20 kinds of hormones such as epinephrine, norepinephrine, somatropin, gonadotropin releasing hormone, add the traumatic of operation of opening cranium, make the feasibility of this model practical operation be under suspicion, this model can not reflect only that body MT level reduces the influence to body after all; Although over past ten years, the applying transgene animal, gene targeting and gene knockout technology, research means concrete hormone or hpr gene have been fine to, thereby accurately copying single hormone or hpr gene crosses expression (hyperfunction) or does not have the expression animal model of (lowly), be resected to a kind of really qualitative leap of gene knockout from body of gland, but same problem also appears in the second class animal model, because the MT receptor exists different subtype, it is very extensive to distribute in mammalian organism, the research and the imperfection of its corresponding effect function, and body MT concentration affects MT receptor mrna level, also therefore bring certain puzzlement to practice; Continuous light model manufacturing cycle is very long, must consider seasonal effect and age factor, and promptly MT can progressively reduce this fact in its body of follower age growth, thereby need be further perfect.Continuing low animal model so make up a MT in serum, is the task of top priority with the corresponding animal model of clinical symptoms especially, also is the important method and the means of associated disease prevention of research and treatment.
Summary of the invention
Technical problem to be solved by this invention is that this area is difficult to set up the low animal model of MT in serum.The technical scheme that solves this technical problem provides the construction method that a kind of level of serum melatonin continues low animal model.The bitter cold feeding with Chinese medicine animal that this method is to use, continuous 5～15 days.
Wherein, above-mentioned bitter cold Chinese medicine is at least a in Radix Et Rhizoma Rhei, Natrii Sulfas, Folium Sennae or the DACHENGQI TANG.
Wherein, the consumption of above-mentioned bitter cold Chinese medicine be every day every 10g body weight 0.2～2.0ml soak decocting liquid, every ml soaks decocting liquid and contains every kind of crude drug 1g.
Also use dispelling the stagnated QI feeding with Chinese medicine animal when wherein, stating bitter cold Chinese medicine in the use.
Wherein, above-mentioned dispelling the stagnated QI Chinese medicine is at least a in Fructus Aurantii Immaturus, Cortex Magnoliae Officinalis or the Semen Arecae.
Also use Semen Armeniacae Amarum or Rhizoma Coptidis feeding animals when wherein, stating bitter cold Chinese medicine in the use.
Wherein, the pH value of above-mentioned Chinese medicine of feeding is adjusted to 2≤pH＜7.
Wherein, the temperature of above-mentioned Chinese medicine of feeding is adjusted into 4 ℃.
Wherein, state in the use feeding with Chinese medicine the time return the animal subcutaneous injection of reserpine, consumption is 0.08～8mg/Kgd, continuous 5～15 days.
Animal described in the said method is common laboratory animal, as mice, rat, donkey, rabbit, pig, dog or Cavia porcellus etc.
The present invention also provides the purposes of bitter cold Chinese medicine in the low medicine of the clear melatonin levels of preparation hyperamization.Above-mentioned bitter cold Chinese medicine mainly refers to Radix Et Rhizoma Rhei, Natrii Sulfas, Folium Sennae or DACHENGQI TANG.
The inventive method beneficial effect is that step is easy, and energy is successful and repeatably set up animal modes with continuous low level of serum melatonin, has good application prospects.
Description of drawings
Fig. 1 is that the level of Radix Et Rhizoma Rhei model mice serum melatonin of the 1st batch of (n=15) modeling is with reference to the percentage rate of normal control group melatonin value.
*: compare P＜0.05 with the normal control group.
Fig. 2 is that the level of Radix Et Rhizoma Rhei model mice serum melatonin of the 2nd batch of (n=15) modeling is with reference to the percentage rate of normal control group melatonin value.
*: compare P＜0.05 with the normal control group.
Fig. 3 is that the level of Radix Et Rhizoma Rhei model mice serum melatonin of the 3rd batch of (n=15) modeling is with reference to the percentage rate of normal control group melatonin value.
*: compare P＜0.05 with the normal control group.
The specific embodiment
Below by specific description of embodiments of the present invention the explanation but do not limit the present invention.
The basis of the inventive method is bitter cold feeding with Chinese medicine bitter cold purgative therapies such as Radix Et Rhizoma Rhei, Folium Sennae.The most frequently used medicine is Radix Et Rhizoma Rhei, Natrii Sulfas, Folium Sennae, DACHENGQI TANG etc.Get crude drug in the time of concrete enforcement, be soaked in water, section back decocting, 80 ℃ are condensed into every ml and contain every kind of crude drug 1g, and every day, the oral administration gavage Radix Et Rhizoma Rhei soaked decocting liquid, the every 10g body weight of dosage 0.4ml, continuous 8～15 days.
Can also add use on the superincumbent basis and adopt the Fructus Aurantii Immaturus of dispelling the stagnated QI, sending down the abnormal ascending QI, Cortex Magnoliae Officinalis, Semen Arecae etc., also can use the Cortex Magnoliae Officinalis three thing soup (Cortex Magnoliae Officinalis, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei) of compound recipe, make decoct and irritate stomach, or make the medicine forage feed, consumption is with reference to bitter cold Chinese medicine.
On above-mentioned basis, can also add sending down the abnormal ascending QI medicines such as using root of subprostrate sophora, Flos Inulae, Semen Arecae and increase the dynamics that injures one's stomach.Perhaps add with Rhizoma Coptidis, bitter temperature medicine Semen Armeniacae Amarum.
For increasing the cold of medicine, the medicine of feeding can be handled to 4 ℃ re-using.Perhaps it is processed into the acidity of 2≤pH＜7.The method has that direct bitter cold is rushed down down, the effect of consumption gas dispelling the stagnated QI, and the increasing of the dosage of modeling simultaneously, absorbs and reduces the nutrition intake that the influence and the discharge function of small intestinal absorption function made animal, and discharging increases, and the finite quantity Nutrition.Reduce the effect of MT in serum concentration.
In order further to improve effect, can add again with the injection reserpine.
Embodiment
1, modeling method and grouping
The laboratory animal Kunming mouse, body weight 20 ± 2g is provided by animal housing of Chengdu University of Traditional Chinese Medicine.Animal is divided into model group and normal control group at random, 15 every group.
Get Radix Et Rhizoma Rhei 100g, soak with the 500ml deionized water, the back 80 ℃ of heating in water bath of cutting into slices are condensed into every ml and contain the 1g crude drug, and every day, the oral administration gavage Radix Et Rhizoma Rhei soaked decocting liquid 1 time, the every 10g body weight of dosage 1.0ml, continuous 8 days; Detect morning 1～2 o'clock mice serum MT concentration after the modeling.
2, observation index
The eyeball blood sampling is gone in the 2nd day morning 1～2nd after the last administration, and 4 ℃ of refrigerators leave standstill, 4 ℃ of centrifugal 10min (2500r/min), and separation of serum is to be measured in-80 ℃ of preservations.This is tested in Wenjiang, 11～December Chengdu in 2008 and carries out.Adopt the ELISA method, Bio-Rad 680 type microplate reader detect MT (mice melatonin ELISA Kit, Wuhan China-US Science and Technology Co., Ltd), and each increment originally carries out two point and measures detection MT content, and by specification is operated.
3, statistical procedures method
The processing of data uses the SPSS11.0 statistical software to handle.
4, result
Fig. 1 is the percentage rate of the content of Radix Et Rhizoma Rhei model of spleen deficiency mice serum MT with reference to the normal control class value, and the result has shown that at morning 1～2 points Radix Et Rhizoma Rhei model group mice serum MT concentration is compared P＜0.05 with the normal control group.The modeling success.
For the repeatability of research the inventive method, carried out twice modeling as stated above again, mice MT in serum in the morning concentration that detects after the modeling is seen Fig. 2 and Fig. 3, the result shows equal modeling success.
Claims (1)
1. the purposes of bitter cold Chinese medicine in the low animal model of preparation level of serum melatonin, described bitter cold Chinese medicine is Radix Et Rhizoma Rhei, consumption be every day every 10g body weight 0.2～2.0ml soak decocting liquid, every ml soaks decocting liquid and contains crude drug 1g, feeding animals is 5～15 days continuously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009103031706A CN101574409B (en) | 2009-06-11 | 2009-06-11 | Method of using bitter cold traditional Chinese medicine to construct animal modes with continuous low level of serum melatonin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009103031706A CN101574409B (en) | 2009-06-11 | 2009-06-11 | Method of using bitter cold traditional Chinese medicine to construct animal modes with continuous low level of serum melatonin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101574409A CN101574409A (en) | 2009-11-11 |
| CN101574409B true CN101574409B (en) | 2011-04-20 |
Family
ID=41269569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009103031706A Expired - Fee Related CN101574409B (en) | 2009-06-11 | 2009-06-11 | Method of using bitter cold traditional Chinese medicine to construct animal modes with continuous low level of serum melatonin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101574409B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144711A (en) * | 2010-02-08 | 2011-08-10 | 上海百树生物科技有限公司 | Senna leaf extract feedstuff additive and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724036A (en) * | 2004-07-20 | 2006-01-25 | 中山大学 | Medicine for improving internal secreting melatonin, and application of its related herb medicine |
-
2009
- 2009-06-11 CN CN2009103031706A patent/CN101574409B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724036A (en) * | 2004-07-20 | 2006-01-25 | 中山大学 | Medicine for improving internal secreting melatonin, and application of its related herb medicine |
Non-Patent Citations (4)
| Title |
|---|
| 丁慧登等.肾阴虚证的现代研究进展.《现代生物医学进展》.2008,第8卷(第1期), * |
| 吴建胜等.褪黑激素对应激性溃疡大鼠胃黏膜诱生型一氧化氮合酶表达的影响.《胃肠病学》.2004,第9卷(第5期), * |
| 王剑等.褪黑素与肾阴的关系探讨.《中医药导报》.2005,第11卷(第10期), * |
| 贺燕等.脾虚动物模型的造模方法及其病理改变.《山西中医学院学报》.2008,第9卷(第1期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101574409A (en) | 2009-11-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1289137C (en) | Medicine for treating mammary gland proliferation, its preparation method and application | |
| CN103417846B (en) | Chinese medicine composition of a kind of blood sugar lowering and preparation method thereof | |
| CN101574409B (en) | Method of using bitter cold traditional Chinese medicine to construct animal modes with continuous low level of serum melatonin | |
| CN104042720B (en) | There is the Chinese medicine and application thereof of preventing and treating diabetes complicated depression | |
| CN101574346B (en) | Method of using reserpine to construct animal model with continuous low level of serum melatonin | |
| CN101028348A (en) | Chinese medicinal capsule, its production and quality controlling method | |
| CN1213750C (en) | New use of bergenin in treating chronic prostatitis and proliferative diseases | |
| CN103977300B (en) | One has the pancreatitic Traditional Chinese medicine compound composition for the treatment of and application thereof | |
| CN100344312C (en) | Prepn process of granule for treating children's hyperkinesia and its quality control method | |
| CN102335362B (en) | Traditional Chinese medicine for treating insulin resistance of type 2 diabetes | |
| CN102000250A (en) | Black-bone chicken health-care medicament as well as preparation method and applications thereof | |
| CN101579407A (en) | Method for preparing animal models with low serum melatonin level by using acidoid | |
| CN104435931B (en) | One kind analgesic patch and preparation method thereof | |
| CN104784354B (en) | A kind of Chinese medicine acupoint injection therapy liquid and its preparation method treating migraine | |
| CN101829290B (en) | Medicament for treating diabetic peripheral neuropathy and preparation method thereof | |
| CN115518133B (en) | Traditional Chinese veterinary medicine composition for treating cat stranguria | |
| CN101601863A (en) | A kind of construction method of diabetes animal model | |
| CN102641349B (en) | Traditional Chinese medicine composite for resisting ephedrine damage and production technology thereof | |
| CN1270763C (en) | Preparation for removing blood stasis keeping kidney essence | |
| CN201596251U (en) | Medicine coating device for percutaneous scarification | |
| CN101612251A (en) | Control the Chinese medicine of primary hepatocarcinoma | |
| CN1623557A (en) | Application of gold ear mycelium polysaccharide for lowering blood fat and blood sugar | |
| CN105521327A (en) | Medicinal composition and application thereof | |
| CN1274339C (en) | Anqi preparation | |
| CN2827406Y (en) | Multi-point syringe needle on throat mucosa |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110420 Termination date: 20140611 |
|
| EXPY | Termination of patent right or utility model |