CN1657444A - Super-concentrated algae bacterium micro-ecological balance suspension type water quality modifier and preparation method thereof - Google Patents
Super-concentrated algae bacterium micro-ecological balance suspension type water quality modifier and preparation method thereof Download PDFInfo
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- CN1657444A CN1657444A CN 200510041775 CN200510041775A CN1657444A CN 1657444 A CN1657444 A CN 1657444A CN 200510041775 CN200510041775 CN 200510041775 CN 200510041775 A CN200510041775 A CN 200510041775A CN 1657444 A CN1657444 A CN 1657444A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 124
- 238000002360 preparation method Methods 0.000 title claims abstract description 62
- 239000000725 suspension Substances 0.000 title claims abstract description 33
- 239000003607 modifier Substances 0.000 title abstract 4
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The invention discloses a micro-ecological balance suspension type water quality modifier for hyperconcentrated algae bacteria and a preparation method thereof, wherein the water quality modifier comprises the following raw materials in percentage by mass: the number of cells is more than or equal to 102030-85% of CFU/ml algae concentrated solution; the number of cells is more than or equal to 104010-60% of CFU/ml bacterial concentrated solution; 4-20% of special nutrient solution for phycomycetes; 0.10 to 0.25 percent of special preservation suspending agent; the total amount of the raw materials is 100 percent; the water quality modifier can be applied to water to purify water, increase dissolved oxygen in water, and reduce ammonia Nitrogen (NH) in water3-N) and nitrous Nitrogen (NO)2 －) Etc.; meanwhile, natural bait for aquatic animals is provided, and the production performance of water is improved; controlling the propagation and growth of harmful algae and fungi, promoting the proliferation of beneficial bacteria, and limiting pathogenic bacteria of aquatic animalsThe propagation quantity of the preparation plays a role in ecological disease prevention, so the preparation has wide application value, no pollution, no influence on water environment, low dosage, low cost and convenient use.
Description
Technical field
The present invention relates to biological probiotics and field of environment protection, be particularly suitable for a kind of novel ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent and preparation method that water surrounding improvement such as culture fishery and purification of water quality are used.
Background technology
In the aquaculture, because the pond is aging, the deposition of substrate bait and a large amount of ight soil, in the water body to a large amount of accumulation such as aquatic animal objectionable impurities such as ammonia-state nitrogen, nitrite nitrogen, poisonous algae toxin, hydrogen sulfide, methane, particularly aquatic animal disease is cultured in life-time service sterilizing agent and the control of some killing livestock property chemicalses, cause the beneficial microorganism and the useful algae mass mortality of some purification of water qualitys, make the water body environment detergent power be seriously damaged, the water body self-purification ability forfeiture, aquaculture water worsens more, disease is on the rise, and is explosive propagation; Simultaneously, frequently use antibiotic, the medicine of killing livestock also to cause a series of environment and social concern in order to prevent and treat disease to take place.As everyone knows, breed fish and support water earlier, the optimization of water body environment is the most important condition that health is breeded fish.In recent years, people begin to attempt using effective microorganism preparation in aquaculture water and improve the breeding ecological environment, suppress pathogenic micro-organism, thereby reduce the generation of disease.This method has obtained good effect in culturing practice.Therefore, use a large amount of useful algae and beneficial bacteria in the water body, can increase the water body dissolved oxygen, reduce ammonia nitrogen, suppress the pathogenic bacterium growth, purify and improve aquaculture water, reduce the generation of disease; Also can provide abundant aquatic animal natural bait, promotion to culture functions such as object growth simultaneously.Therefore, the useful algae and the beneficial bacteria of propagation are the improvement water body environments, realize ecological adjusting, carry out healthy aquaculture, produce the most effective means of green non-pollution fishery products in a large number to culture the waters.
In addition, the preservation of live algae, viable bacteria is mainly preserved by means such as low temperature, is unfavorable for scale operation and use.At present, do not see the algae preparation alive that uses in the production as yet both at home and abroad, and the bacterium probiotics also has only two kinds of formulations: the one, bacterial cultures is concentrated, after the cryodrying,, obtain solid preparation with carrier absorption, mixing, pack or bottling are preserved; The 2nd, bacterial cultures is made bacteria suspension, bottling is preserved.The former is the cost height aborning, needs large-scale freeze-drying and separating device, and product comes into the market because of room temperature storage bacteria living time weak point, effect decline after using; Latter bacterium is thanked to aerogenesis through offspring after a while and causes the Plastic Bottle bottle explosion of expanding, simultaneously, under the bacterium normal temperature death very high, deposit layering in a large number, the shelf time is very short, total plate count reduced more than 80% in 6 months.So, can reduce production costs, need not main equipment investment, production technique is simple, and bacterium and algae can preserve for a long time and the low new preparation of mortality ratio has wide application prospect.
Summary of the invention
The purpose of this invention is to provide a kind of ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent, this improver of water quality uses in the waters, do not influence the quality and the safety of fishery products, and pollution-free, do not influence water surrounding, using dosage is low, cost is little, easy to use.
Another object of the present invention is, the preparation method of above-mentioned ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent is provided.
The technical solution that realizes above-mentioned purpose is, a kind of ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent is characterized in that, contains following quality than raw material:
1) cell quantity 〉=10
20The algae concentrated solution of CFU/ml, 30%～85%;
2) cell quantity 〉=10
40The bacterium concentrated solution of CFU/ml, 10%～60%;
3) phycomycete special nutrient fluid, 4%～20%;
4) special-purpose suspension agent, 0.10%～0.25% preserved;
The summation of above-mentioned raw materials is 100%;
Above-mentioned algae concentrated solution is selected from green alga or diatom;
Described bacterium concentrated solution is the concentrated solution that vinelandii, X401 aerogen, nitrobacteria, nitrite bacteria, yeast are mixed with;
Described special-purpose preservation suspension agent is agar powder, dextran, Sargassum polysaccharides mixture, and ratio is 12: 5: 1;
Described phycomycete special nutrient fluid contains following ingredients and 1000ml water mixes:
NH
4CI???????????????????????????????????0.21g
Ca(H
2PO
2)
2·H
2O+2CaSO
2·H
2O???????0.03g
MgSO
4·7H
2O????????????????????????????0.07g
NaHCO
3???????????????????????????????????0.11g
KCI???????????????????????????????????????0.03g
Fish meal 0.5g～2.0g.
The prescription of the concentrated solution that described vinelandii, X401 aerogen, nitrobacteria, nitrite bacteria, yeast are mixed with is: X401 aerogen 30%, vinelandii 20%, yeast 10%, nitrobacteria 20%, nitrite bacteria 20%.
The preparation method of above-mentioned ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent is characterized in that, is undertaken by following step successively:
The cultivation of a, algae
The green alga (grid algae, chlorella) or the diatom of pure culture are at first cultivated through one-level, secondary expanding species.
The one-level of algae kind is cultivated: under the sterilisable chamber Bechtop, with protect the pure culture grid algae kind on the test tube slant, green alga or the diatom algae kind that chlorella is the algae source is inoculated in the 500ml Erlenmeyer flask, put in the illumination box, 25 ℃ of attemperation, illumination illumination is 5600Lx, use x06 nutrient solution prescription, shake every day bottle 4-6 time, cultivate 72h after algae concentration above 10
6Obtain one-level algae culture liquid during CFU/ml.
The secondary of algae kind is cultivated: under aseptic technique, utilize the 5kg glass jar, carry out the secondary amplification cultivation in the algal species cultivation chamber.The one-level algae liquid capacity of general inoculation and newly to join the ratio of cultivating liquid measure be 1: 2～1: 3,25 ℃ of attemperation, illumination illumination is 5600Lx, uses x06 nutrient solution prescription, shakes every day bottle 4-6 time, algae concentration is above 10 behind the cultivation 48-72h
6Obtain secondary algae culture liquid during CFU/ml.
Mass production is cultivated: inoculate under aseptic technique, utilize 50kg plastic tank or 5-10m
3Cement pit, cultivate at algal species cultivation chamber mass production.The secondary algae liquid capacity of general inoculation and newly to join the ratio of cultivating liquid measure be 1: 20～1: 30,25 ℃ of attemperation, illumination illumination is 5600Lx, use x06 nutrient solution prescription, nutrient solution is inflated cultivation through disinfecting, and the cultivation pool capacity is big, can take gradation to add the method for nutrient solution, for the first time cultivating the water yield is about 60% of Total Water, cultivates after 2-3 days, and frustule has been bred bigger density, can add nutrient solution 40% again and continue to cultivate, algae concentration surpasses 10
6Obtain algae culture liquid during CFU/ml.The x06 nutrient solution contains following ingredients and 1000ml water mixes:
Urea 0.133g
Ca(H
2PO
4)
2·H
2O??????????????????0.020g
Phosphoric acid 0.020ml
MgSO
4·7H
2O?????????????????????????0.10g
NaHCO
3???????????????????????????????0.10g
KCl???????????????????????????????????0.033g
The FeSO of 1% aqueous solution
40.20ml
The calcium chloride 0.05ml of 5% aqueous solution
0.05% fish meal leach liquor 0.5ml;
The cultivation of b, bacterium
X401 aerogen, vinelandii, yeast, nitrobacteria, the nitrite bacteria bacterial classification of pure culture are at first cultivated through one-level, secondary expanding species.
The one-level of bacterial classification is cultivated: under the sterilisable chamber Bechtop, to protect X401 aerogen kind on the test tube slant respectively, with nitrogen bacterium, yeast, nitrobacteria, nitrite bacteria bacterial classification inoculation in the 500ml Erlenmeyer flask that fills the ordinary broth nutrient solution, put in the water bath with thermostatic control vibrator, 25 ℃ of attemperation, oscillation frequency is 50 times/min, behind the cultured continuously 48h, bacterial concentration surpasses 10
15Obtain the one-level inoculum during CFU/ml.
The secondary of bacterial classification is cultivated: under the sterilisable chamber Bechtop, respectively with the X401 aerogen, be inoculated in the 5kg glass jar that fills the ordinary broth nutrient solution with the one-level inoculum of nitrogen bacterium, yeast, nitrobacteria, nitrite bacteria, one-level bacteria suspension volume and newly to join the ratio of cultivating liquid measure be 1: 5～1: 10,25 ℃ of attemperation, manually shake bottle every day 5 times, behind the cultured continuously 48h, bacterial concentration surpasses 10
15Obtain the secondary inoculum during CFU/ml.
Mass production is cultivated: under the sterilisable chamber Bechtop, secondary inoculum with X401 aerogen, vinelandii, yeast, nitrobacteria, nitrite bacteria is inoculated in the 50-500kg container that fills the ordinary broth nutrient solution respectively, the volume of secondary inoculum and newly to join the ratio of cultivating liquid measure be 1: 10～1: 20,25 ℃ of attemperation, after 48-72h is cultivated in continuous charge, when bacterial count 〉=10
20Behind the CFU/ml, obtain inoculum;
C, algae, inoculum concentrate
Add the flocculation agent tschermigite respectively in algae, inoculum, making concentration is 0.05%, stirs 30 minutes, after the standing over night, removes supernatant liquor, cultivates respectively 1-2 days again, puts super-magnum centrifuge centrifugal concentrated thick algae, bacterium enriched material;
D, mixing concentrate the preparation of bacterium liquid
After the X401 aerogen cultivated respectively, vinelandii, yeast, nitrobacteria, nitrite bacteria flocculation, centrifugal concentrating, mix to concentrate bacterium liquid by following prescription, its prescription is:
X401 aerogen 30%
Vinelandii 20%
Yeast 10%
Nitrobacteria 20%
Nitrite bacteria 20%
E, special-purpose preparation of preserving suspension agent
With agar powder, dextran, Sargassum polysaccharides mixture, its ratio is mixing in 12: 5: 1, be mixed by the described phycomycete special nutrient fluid of claim 1 mass ratio again, place the stainless steel jacketed kettle, 100 ℃ of temperature controls, heated and stirred 5-10 minute, be incubated then 45 ℃ down standby, promptly obtain the special-purpose suspension agent of preserving;
F, algae bacteria microecological equipment suspension agent preparation
With useful algae concentrated solutions such as green alga, diatoms, vinelandii, aerogen, nitrobacteria, nitrite bacteria, yeast mix concentrated bacterium liquid, the phycomycete special nutrient fluid, the special use of above-mentioned preparation is preserved three kinds of compositions of suspension and is mixed according to the described mass ratio of claim 1, heat 35 ℃-40 ℃, stirred 5 minutes with homogenizer, restir is 5 minutes after the cooling room temperature, promptly gets the ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent.
The main effect of ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent of the present invention is:
1) purifies water.
Algae is improved the metabolism of aquatic animal by the dissolved oxygen of photosynthesis increase water body, strengthens aerobic bacteria to degradation of organic matter; Algae can utilize the organic CO that produces of degradation by bacteria
2, quicken fissiparity, can reduce N, the P content in the water body and strengthen enriched in metals ionic ability; The probiotics of a large amount of propagation is then by ammonification, nitrification, denitrification, nitrogen fixation, under the condition that dissolved oxygen enriches, with the ammonia-state nitrogen (NH in the water body
3-N) and nitrite nitrogen (NO
2 -) wait poisonous ammonia nitrogen to be converted into nitric nitrogen (NO
- 3), by algae is utilized, thereby play the effect that purifies water; In addition, the phosphorus of solubilised state is formed particulate form phosphorus by suspended particle absorption in the water body, transfers precipitation to through flocculation reaction, reduces the content of available phosphorus in the water body, thus the breeding growth of poisonous algae such as the eutrophication of control water and blue-green algae, restriction vitriol and hydrogen sulfide (H
2S) etc. generation.
2) provide the aquatic animal natural bait, improve the water body production performance
Algae is the natural bait of fish, shrimp crab, and this preparation is rich in useful algae such as green alga, diatom, can breed rapidly, grow at water body, provides abundant bait for culturing object, promotes its growth.Simultaneously, probiotic bacterium can suppress the growth of pathogenic micro-organism in the aquaculture water, and progressively is formed with the profitable strain that benefits aquatic animal to grow, reduces the generation of disease, improves the water body production performance.
3) breeding and the growth of control water body harmful algae, mushroom
Because what said preparation adopted is highly enriched useful algae, bacterium, can in water body, breed in a large number rapidly, in aquatic ecosystem, occupy superiority, can suppress the breeding and the growth of its harmful algae, mushroom.When water body environment changes, can cause the particularly variation of plant plankton population of water body planktonic organism, a large amount of breedings of harmful algae such as blue-green algae etc. bring disaster to production.If the use said preparation, green algas such as grid algae, chlorella breed in a large number, just can suppress the growth and breeding of harmful algae, and the final useful algae population of preponderating is improved water body environment.Experimental study finds that probiotics is the propagation of X401 aerogen (separating acquisition from soil in this research), vinelandii particularly, can strengthen a large amount of breedings of useful algae, has also limited the breeding quantity of aquatic pathogenic bacteria simultaneously, plays the purpose of ecological diseases prevention.
4) provide useful algae kind for the water bodys such as pond after the drug use of killing livestock.
At present, in order to kill in the water body to the deleterious biology of aquatic animal, clean up the pond, kill harmful algae and used when killing parasite many medicines of killing livestock, result to cause the death of useful algae and mushroom in the pond, use said preparation can effectively replenish these useful phycomycetes.
5) plant plankton, the waterplant for eutrophication water provides photosynthetic CO
2
Studies confirm that in a large number behind the body eutrophication, algae and the breeding of waterplant raised growth make CO in the water body
2Shortage, photosynthesis generation obstacle is a reason that causes a large amount of algae death.
Isolating X401 aerogen, yeast all can decompose the organic CO of generation among the present invention
2, supply with the required energy of algae photosynthesis; In addition, vinelandii also can change into the free nitrogenous source by the organonitrogen of utilizations such as algae, quicken the breeding of algae, play the effect of improvement water quality.
Being applied to water quality improvement with hyperconcentration algae preparation way alive is one of principal feature of this research.Adopt some special processing methodes, algae, bacterium are concentrated, and long-time sealing is kept at and contains in the special nutrition preservation liquid container, makes phycomycete be in dormant state, and suppress, slow down the growth and breeding of phycomycete, overcome the difficult problem that phycomycete can not prolonged preservation.Simultaneously, the gas that phycomycete is very low in the container metabolism produces is kept a kind of steady state of relative equilibrium, both the O that produces in photosynthesis of the algae in the preparation
2Can be utilized by the oxygen consumption bacterium, and the CO that mushroom discharges
2Absorbed by algae photosynthesis again, make preparation be able to long preservation, and overcome the defective of microniological proudcts flatulence bottle explosion in the past, this is the another principal feature of the research.Use the preservation suspension agent of forming by agar powder, dextran, Sargassum polysaccharides mixture in the research, employing cell suspension theory, make algae alive, bacterium high-density evenly float on a liquid, the necrocytosis that can not precipitate, layering and accumulation causes takes place, therefore, this suspension agent can make algae, bacterium preserve for a long time, and keeps its vigor.
The using method of preparation of the present invention and consumption:
Preparation specification: useful algae content 〉=10
15CFU/ml, probiotics content 〉=10
35CFU/ml.Directly splash during use in the pond, using dosage is: 2000 milliliters of/mu rice.Perhaps can use the fast breeding culture medium that provides, the pond water that adds 1000 times was splashed in large container internal breedings such as little cement pit or plastic tub earlier in 2-4 days again, and effect can be better.
4, the present invention compared with prior art has following beneficial effect:
Algae alive, the viable bacteria of the present invention's development have dual water quality improvement effect, comparing aspect the improvement water quality with the microbial preparation of other pure single bacterium, its principal feature is: have useful algae alive, and adopt the hyperconcentration technology of bacterium, algae, its cell quantity is 10 of general other microbial preparation
40Doubly; Because of having algae alive, viable bacteria, can decompose organic matter, utilize objectionable impuritiess such as ammonia nitrogen, produce a large amount of oxygen, its purification of water quality efficient is significantly higher than the probiotics that only contains bacterium; Algae after a large amount of propagation also is that economic animal, particularly fish, shrimp crab in the water provides abundant natural bait at purifying water body simultaneously, and this is that general probiotics is irrealizable; The useful green alga that preparation provides, bacterium breed in water body in a large number, and beneficial bacteria not only can promote a large amount of propagation of useful algae, and can suppress harmful algae and growth of pathogenic bacteria breeding, realize the purpose of ecological diseases prevention; Can provide, replenish useful algae kind for the water bodys such as pond that used the medicine of killing livestock; X401 aerogen and yeast decompose the organic CO that produces
2Supply with the algae and the photosynthetic energy of waterplant, vinelandii can change into the free nitrogenous source by the organonitrogen of plant utilizations such as algae, have quickened the breeding of algae, play the rich water effect, and other probiotics does not all have these functions.
In addition, said preparation is compared with existing probiotics, and the main difference part is: adopt hyperconcentration live algae, bacteria preparation mode, solved algae alive, bacterium, particularly algae the expand problem of bottle of prolonged preservation and probiotics aerogenesis aborning.
So, the ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent can substitute the probiotics that other bacterium is formed fully on producing, can be widely used in aquaculture wateies such as various fish, shrimp crab, improvement water quality, reduce the generation of aquatic animal disease, simultaneously can provide abundant natural bait, have good social and economic benefit.
Embodiment
The present invention is described in further detail for embodiment that provides below in conjunction with the contriver and test embodiment, but the present invention is not limited to these embodiment, and all equivalent transformations that is carried out in formula range of the present invention all belong to protection scope of the present invention.
Embodiment 1:
The ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent by mass ratio in 100 parts: useful algae concentrated solution such as green alga (cell quantity 〉=10
20Beneficial bacteria concentrated solution (cell quantity 〉=10 such as CFU/ml) 85 parts, vinelandii
40CFU/ml) 10 parts, 4.75 parts of special nutrient fluids, preserve suspension agent (agar powder, dextran, Sargassum polysaccharides mixture, ratio are 12: 5: 1) 0.25 part.
Its preparation method carries out according to the following step:
The cultivation of a, algae
The green alga (grid algae, chlorella) or the diatom of pure culture are at first cultivated through one-level, secondary expanding species.
The one-level of algae kind is cultivated: under the sterilisable chamber Bechtop, with protect the pure culture grid algae kind on the test tube slant, green alga or the diatom algae kind that chlorella is the algae source is inoculated in the 500ml Erlenmeyer flask, put in the illumination box, 25 ℃ of attemperation, illumination illumination is 5600Lx, use x06 nutrient solution prescription, shake every day bottle 4-6 time, cultivate 72h after algae concentration above 10
6Obtain one-level algae culture liquid during CFU/ml.
The secondary of algae kind is cultivated: under aseptic technique, utilize the 5kg glass jar, carry out the secondary amplification cultivation in the algal species cultivation chamber.The one-level algae liquid capacity of general inoculation and newly to join the ratio of cultivating liquid measure be 1: 2～1: 3,25 ℃ of attemperation, illumination illumination is 5600Lx, uses x06 nutrient solution prescription, shakes every day bottle 4-6 time, algae concentration is above 10 behind the cultivation 48-72h
6Obtain secondary algae culture liquid during CFU/ml.
Mass production is cultivated: inoculate under aseptic technique, utilize 50kg plastic tank or 5-10m
3Cement pit, cultivate at algal species cultivation chamber mass production.The secondary algae liquid capacity of general inoculation and newly to join the ratio of cultivating liquid measure be 1: 20～1: 30,25 ℃ of attemperation, illumination illumination is 5600Lx, uses x06 nutrient solution prescription, and the nutrient solution process is disinfected, and inflates cultivation, and algae concentration is above 10
6Obtain algae culture liquid during CFU/ml.
The cultivation of b, bacterium
X401 aerogen, vinelandii, yeast, nitrobacteria, the nitrite bacteria bacterial classification of pure culture are at first cultivated through one-level, secondary expanding species.
The one-level of bacterial classification is cultivated: under the sterilisable chamber Bechtop, to protect X401 aerogen kind on the test tube slant, vinelandii, yeast, nitrobacteria, nitrite bacteria bacterial classification inoculation respectively in the 500ml Erlenmeyer flask that fills the ordinary broth nutrient solution, put in the water bath with thermostatic control vibrator, 25 ℃ of attemperation, oscillation frequency is 50 times/min, behind the cultured continuously 48h, bacterial concentration surpasses 10
15Obtain the one-level inoculum during CFU/ml.
The secondary of bacterial classification is cultivated: under the sterilisable chamber Bechtop, one-level inoculum with X401 aerogen, vinelandii, yeast, nitrobacteria, nitrite bacteria is inoculated in the 5kg glass jar that fills the ordinary broth nutrient solution respectively, one-level bacteria suspension volume and newly to join the ratio of cultivating liquid measure be 1: 5～1: 10,25 ℃ of attemperation, manually shake bottle every day 5 times, behind the cultured continuously 48h, bacterial concentration surpasses 10
15Obtain the secondary inoculum during CFU/ml.
Mass production is cultivated: under the sterilisable chamber Bechtop, secondary inoculum with X401 aerogen, vinelandii, yeast, nitrobacteria, industry nitrobacteria is inoculated in the 50-500kg container that fills the ordinary broth nutrient solution respectively, the volume of secondary inoculum and newly to join the ratio of cultivating liquid measure be 1: 10～1: 20,25 ℃ of attemperation, after 48-72h is cultivated in continuous charge, when bacterial count 〉=10
20Behind the CFU/ml, obtain inoculum;
C, algae, bacterium concentration method
Add the flocculation agent tschermigite respectively in algae, inoculum, making concentration is 0.05%, stirs 30 minutes, after the standing over night, remove supernatant liquor, cultivated respectively again 1-2 days, put super-magnum centrifuge centrifugal concentrate thick algae, bacterium enriched material, and carry out cell counting.
D, mixing concentrate the preparation of bacterium liquid
After the X401 aerogen cultivated respectively, vinelandii, yeast, nitrobacteria, nitrite bacteria flocculation, centrifugal concentrating, become to mix to concentrate bacterium liquid (X401 aerogen 30%, vinelandii 20%, yeast 10%, nitrobacteria 20%, nitrite bacteria 20%) by formulated
E, special-purpose preparation of preserving suspension agent
With agar powder, dextran, Sargassum polysaccharides mixture, its ratio is mixing in 12: 5: 1, be mixed by the described phycomycete special nutrient fluid of claim 1 mass ratio again, place the stainless steel jacketed kettle, 100 ℃ of temperature controls, heated and stirred 5-10 minute, be incubated then 45 ℃ down standby, promptly obtain the special-purpose suspension agent of preserving;
F, algae bacteria microecological equipment suspension agent preparation
Useful algae concentrated solution (cell quantities 10 such as weighing green alga
20CFU/ml) 80kg, X401 aerogen 3kg, vinelandii 2kg, yeast 1kg, nitrobacteria 2kg, nitrite bacteria 2kg add in the agitator respectively and mix; Weighing agar powder 133g, dextran 56g, Sargassum polysaccharides 11g add in the special nutrient fluid of 10Kg in succession, place the stainless steel jacketed kettle, 100 ℃ of temperature controls, heated and stirred 5-10 minute, cool off 45 ℃ after, be poured in the phycomycete mixed solution that weighs up, stirred 5 minutes with homogenizer, cooled off 1-2 hour, restir 5 minutes promptly gets the ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent.
Embodiment 2:
The ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent by mass ratio in 100 parts: useful algae concentrated solution such as diatom (cell quantity 〉=10
20Beneficial bacteria concentrated solution (cell quantity 〉=10 such as CFU/ml) 60 parts, vinelandii
40CFU/ml) 30 parts, 9.85 parts of special nutrient fluids, preserve suspension agent (agar powder, dextran, Sargassum polysaccharides mixture, ratio are 12: 5: 1) 0.15 part.Its preparation method together
Embodiment 1.
Embodiment 3:
The ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent by mass ratio in 100 parts: useful algae concentrated solution such as green alga (cell quantity 〉=10
20Beneficial bacteria concentrated solution (cell quantity 〉=10 such as CFU/ml) 50 parts, vinelandii
40CFU/ml) 35 parts, 14.80 parts of special nutrient fluids, preserve suspension agent (agar powder, dextran, Sargassum polysaccharides mixture, ratio are 12: 5: 1) 0.20 part.Its preparation method together
Embodiment 1.
Embodiment 4:
The ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent by mass ratio in 100 parts: useful algae concentrated solution such as diatom (cell quantity 〉=10
20Beneficial bacteria concentrated solution (cell quantity 〉=10 such as CFU/ml) 30 parts, vinelandii
40CFU/ml) 55 parts, 14.9 parts of special nutrient fluids, preserve suspension agent (agar powder, dextran, Sargassum polysaccharides mixture, ratio are 12: 5: 1) 0.10 part.Its preparation method together
Embodiment 1.
Embodiment 5:
The ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent by mass ratio in 100 parts: useful algae concentrated solution such as green alga (cell quantity 〉=10
20Beneficial bacteria concentrated solution (cell quantity 〉=10 such as CFU/ml) 40 parts, vinelandii
40CFU/ml) 55 parts, 4.9 parts of special nutrient fluids, preserve suspension agent (agar powder, dextran, Sargassum polysaccharides mixture, ratio are 12: 5: 1) 0.10 part.Its preparation method is with embodiment 1.
Embodiment 6:
The ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent by mass ratio in 100 parts: useful algae concentrated solution such as diatom (cell quantity 〉=10
20Beneficial bacteria concentrated solution (cell quantity 〉=10 such as CFU/ml) 70 parts, vinelandii
40CFU/ml) 24 parts, 5.9 parts of special nutrient fluids, preserve suspension agent (agar powder, dextran, Sargassum polysaccharides mixture, ratio are 12: 5: 1) 0.10 part.Its preparation method is with embodiment 1.
Embodiment 7:
The ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent by mass ratio in 100 parts: useful algae concentrated solution such as diatom (cell quantity 〉=10
20Beneficial bacteria concentrated solution (cell quantity 〉=10 such as CFU/ml) 50 parts, vinelandii
40CFU/ml) 35 parts, 14.75 parts of special nutrient fluids, preserve suspension agent (agar powder, dextran, Sargassum polysaccharides mixture, ratio are 12: 5: 1) 0.25 part.Its preparation method together
Embodiment 1.
Experimental example: ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent production result of use test
On April 10th, 2003 between 10 days October in 2003, selected 6 ponds to carry out " ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent " production test in fishing ground, Mei County, Baoji, the pond is the old pond in more than 20 year of construction, pond sludge is very thick, the acute for many years height of nitrite, ammonia-nitrogen content does not fall, the time have the general pool to take place, annual fish disease outburst is frequent, breeding production is caused very big loss, after using said preparation, comparing with the contrast pond, is that volume increase or the generation that reduces disease all produce significant effect, and relevant testing data is as follows:
1. materials and methods
The ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent, specification, 1000ml/ bottle, content: green algas such as grid algae, chlorella: 〉=10
15CFU/ml, beneficial bacteria sum 〉=10
35CFU/ml.Using dosage is: 2000ml/ mu rice.
Test site: fishing ground, Mei County, Baoji.6 areas are 5 mu old pond, and 1, No. 2 carp, crucian carp, grass, bream, silver carp, bighead are raised together with in the pond, cultivate fingerling by spray, No. 3 pond control groups; 4, carp, crucian carp, grass, bream, silver carp, bighead are raised together with in No. 5 ponds, culture adult fish by fingerling, No. 6 pond control groups.
Before the use, applying fertilizer after the unification of 1-6 pond added water, mainly is chicken manure, carbon ammonia etc.1, the ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent is used simultaneously by operation instruction in 2,4, No. 5 ponds, and 3, No. 6 contrast is established in the pond.Use the back to measure every index respectively, and every 30d re-uses 1 time in breeding production, measure the plant plankton biomass every 20d at 3d, 4d, 5d, 6d, 7d.In the test, be unified in 10d, 1,2, No. 3 spray is put in the pond in a suitable place to breed, every mu 150,000 tail; 4, the fingerling that mean body weight is 120g is put in 5, No. 6 ponds in a suitable place to breed, puts 1200 tails in a suitable place to breed for every mu.Daily administration adopts fertilising, the granulated feed of throwing something and feeding to combine, and refreshes the water periodically, offers oxygenating machine, diseases prevention and treatment, the record management incidence.Section has been used 4 times altogether at the trial.
1.1 plant plankton is quantitative and water transparency is measured
Offshore 1m place and pond central authorities respectively select 1 sampling point to sample with water sampler around in the pond, gather top layer, bottom water respectively, liquid-solid fixed with 1% iodine, leave standstill 24h and concentrate constant volume, cell counting count board microscopic examination counting, according to 1L water formula N=Cs/FsFn * V/v * Pn (N plant plankton quantity, Cs counting frame area mm
2, the area mm in a visual field of Fs
2, the volume mL after the visual field number that Fn counted, V1L water concentrate, the long-pending mL of v counting framework, Pn in the Fn visual field, the plant plankton quantity that is count down to) calculate plant plankton quantity, be converted into biomass (mg/L) by preponderant algae again.And with control group relatively.Each sampling is fixed on to be carried out during 14:00 afternoon.
Adopt the strange Sechi plate method of plug to measure water transparency.
1.2 the inspection of organic matter degradation mensuration and water body preponderant algae at the bottom of the pond
Regularly at the bottom of each pond, pond, take bed mud, detect black mud colour-change situation, measure organic matter degradation ability at the bottom of the pond with this.
Fixed point is gathered water sample, and statistical computation goes out each pond algal kind, quantity, observes production and uses the influence of preparation to the algae and water population period.
1.3 the mensuration of dissolved oxygen, nitrite, ammonia-nitrogen content in the water body
Water sampling during 15:00 adopts the water quality real-time analysis box of department of chemistry of Peking University development to measure dissolved oxygen, nitrite, ammonia-nitrogen content in the afternoon.
1.4 the inspection of bottom water bacteria quantity
20d, 40d, 60d, 80d, 100d, 120d, 140d, 160d, 180d in the breeding production phase gather 1,2, No. 3 pond bottom water sample, use plate count statistics water bottom bacterial species and Changing Pattern.
1.5 fingerling, adult fish growth performance and incidence
Regularly fish for fry, fingerling or adult fish immediately and weigh, check morbidity and administrative situation, and compare with control group.
2 results
2.1 plant plankton is quantitative and water transparency is measured
The sample that offshore 1m place around the pond and pond central authorities respectively select 1 sampling point to adopt is counted calculating plant plankton biomass (mg/L) mean value.Water temperature is at 20-30 ℃.Using the initial 7d of said preparation to the results are shown in following table 1, every 30d re-uses 1 time in breeding production, measures the plant plankton biomass every 20d and the results are shown in Table 2.Find out by table 1, after just having added river, all 6 ponds are at initial 0d, 1d, 1,2,4, No. 5 pond plant plankton biomasss of test group are consistent with transparency and control group 3,6, from 3d～7d, 1, No. 2 pond plant plankton biomasss of test group and transparency and control group 3 show significant difference p＜0.01,4, No. 5 pond plant plankton biomasss of test group and transparency and control group 6 significant difference p＜0.01.Table 2 is the result show, from 1d～180d produces, 1,2,4, No. 5 ponds of test group owing to the former two are from the spray to the fish fry breeding, the plant plankton biomass of every 20d and transparency present 100d and raise in the past, and downtrending appears in 100d, the latter two 4, No. 5 ponds, owing to be to support to the adult fish stage from fingerling, lower to the plant plankton utilization, after 20d, maintain certain level substantially.20d, the 40d, 60d, 80d, 100d, 120d, 140d, 160d, the 180d that are measuring, 1, No. 2 ponds of test group and No. 3 ponds of control group, 4, No. 5 ponds of test group and No. 6 ponds of control group all present significant difference p＜0.01 at plant plankton biomass and transparency.
Table 1 uses pond plant plankton biomass and transparency in the initial 7d of preparation
| The pond numbering | Plant plankton biomass mg/L transparency cm | |||||||
| ??????0d ??mg/L???cm | ??????1d ??mg/L???cm | ??????2d ??mg/L????cm | ??????3d ??mg/L????cm | ??????4d ??mg/L????cm | ??????5d ?mg/L?????cm | ??????6d ??mg/L?????cm | ?????7d ?mg/L????cm | |
| ????1 | ??5.4????60 | ??5.8?????60 | ??9.2?????50 | ??19.8????40 | ??30.6????35 | ??41.5????30 | ??65.5?????25 | ??77.6????20 |
| ????2 | ??5.3????60 | ??5.9?????60 | ??9.5?????50 | ??19.5????40 | ??30.0????35 | ??40.9????30 | ??68.7?????25 | ??78.9????20 |
| ????3 | ??5.3????60 | ??5.8?????60 | ??6.5?????60 | ??10.1????50 | ??14.4????48 | ??19.7????40 | ??25.8?????35 | ??32.4????30 |
| ????4 | ??5.3????60 | ??5.6?????60 | ??9.7?????50 | ??20.4????40 | ??31.2????35 | ??41.6????30 | ??68.4?????25 | ??80.8????20 |
| ????5 | ??5.4????60 | ??5.5?????60 | ??9.1?????50 | ??21.3????40 | ??30.5????35 | ??41.6????30 | ??69.9?????25 | ??78.2????20 |
| ????6 | ??5.3????60 | ??5.7?????60 | ??6.8?????60 | ??11.0????50 | ??15.0????47 | ??19.5????40 | ??26.4?????35 | ??33.3????30 |
Table 2 uses the every 20d of preparation pond plant plankton biomass
| The pond numbering | Plant plankton biomass mg/L transparency cm | |||||||
| 20d mg/L cm | 40d mg/L cm | 80d mg/L cm | 100d mg/L cm | 120d mg/L cm | 140d mg/L cm | 160d mg/L cm | 180d mg/L cm | |
| ?1 | ?125.5???20 | ?132.0???20 | ?130.8???20 | ?132.6???20 | ??120.2???25 | ?101.4???30 | ?106.4???30 | ?98.6????30 |
| ?2 | ?128.9???20 | ?140.2???20 | ?132.4???20 | ?128.0???20 | ??122.8???25 | ?113.9???30 | ?105.5???30 | ?100.7???30 |
| 3 contrasts | ?58.9????35 | ?55.8????35 | ?59.7????35 | ?67.2????30 | ??70.6????30 | ?79.1????30 | ?67.6????35 | ?65.5????35 |
| ?4 | ?136.4???20 | ?133.3???20 | ?130.7???20 | ?121.4???25 | ??113.8???25 | ?112.0???25 | ?119.5???25 | ?112.9???25 |
| ?5 | ?135.0???20 | ?130.5???20 | ?128.6???25 | ?120.3???25 | ??119.2???25 | ?113.8???25 | ?110.4???25 | ?99.89???25 |
| 6 contrasts | ?75.6????30 | ?78.1????30 | ?77.5????30 | ?89.4????30 | ??88.7????30 | ?65.5????35 | ?62.1????40 | ?60.3????40 |
2.2 the inspection of organic matter degradation mensuration and water body preponderant algae at the bottom of the pond
Regularly take bed mud and observe the black mud colour-change at the bottom of each pond, pond, each pond detected result sees Table 3.By table as can be seen, because be to cultivate fingerling, preliminary stage mainly is the fertilising and the soya-bean milk of splashing in 1,2, No. 3 pond, No. 3 pond 120d of control group with the forebay at the bottom of the black mud color do not have noticeable change, but from the later breed of the 120d stage, because the granulated feed of throwing something and feeding, the blackening of sediment of pond color is deepened; And 1,2 test tanks show no black mud layer at 80d, 100d sampling result, and at 120d-180d in the time, owing to use mixed feed to throw something and feed, but because the use said preparation, the black mud color is more shallow and continual and steady, the result who plays a role for preparation; In 4,5, No. 6 adult fish culture ponds, because just using mixed feed at the beginning cultures, 4, No. 5 test pond black mud color ratios begin to shoal before the test, the thickness attenuation, and it is stable always, and all deepen from 60d-80d black mud color, thickness in No. 6 ponds of control group, and between 120d-180d, color is darker, thickness reaches 12-20cm, be the former more than 2 times.In a word, test group and control group all show significant difference p＜0.01 in bed mud black mud color, thickness increase.
At production period, regularly to each pond sample examination algal kind and biomass, find 3-4d after using improver of water quality, promptly can be observed Chi Shui and pure and fresh beautiful yellowish green or dark brown water colour occurs, and keep stable always.Useful algae green alga and diatom account for 90%, and blue-green algae and assorted algae account for 5-10%; Control group green alga and diatom account for 70-80%, and assorted algae accounts for 20-30%, illustrate that this improver of water quality can promote the breeding growth of good unicellular algaes such as diatom, green alga, has the obvious suppression effect to assorted algaes such as blue-green algaes.
Organic matter degradation situation at the bottom of the every 20d of the table 3 use preparation pond
| The pond numbering | Black mud color and luster changing conditions |
| ??0d????20d?????40d?????60d?????80d????100d??????120d?????140d????160d??????180d | |
| 123 contrasts, 456 contrasts | ??+++????++??????+???????+???????-???????-????????+????????+???????+?????????+ ??+++????++??????+???????+???????-???????-????????+????????+???????+?????????+ ??+++????+++?????+++?????+++?????+++?????+++??????++++?????++++????++++??????++++ ??+++????++??????+???????+???????+???????+????????+????????+???????+?????????+ ??+++????++??????+???????+???????+???????+????????+????????+???????+?????????+ ??+++????+++?????+++?????++++????++++????+++++????+++++????+++++???+++++?????+++++ |
Annotate :+expression black mud exists;-expression black mud disappears.The degree of how much representing black mud of "+", multilist shows serious more more.
2.3 the mensuration of dissolved oxygen, nitrite ion, ammonia-nitrogen content in the water body
Table 4 uses pond dissolved oxygen in the initial 7d of preparation, nitrite ion, ammonia-nitrogen content to change (water temperature 20-23 ℃)
| The pond numbering | ??0d ??mg/L | ??1d ??mg/L | ??2d ??mg/L | ??3d ??mg/L | ??4d ??mg/L | ??5d ??mg/L | ??6d ??mg/L | ??7d ??mg/L |
| ??1??DO ??NO 2- ??NH 3-N | ??8.0-9.0 ??0.15 ??1.42 | ??9.0-10.0 ??0.15 ??1.42 | ??10-11 ??0.15 ??1.40 | ??11-12 ??0.10 ??1.10 | ??13-14 ??0.10 ??0.85 | ??13-14 ??0.015 ??0.65 | ??13-14 ??0.010 ??0.55 | ??13-14 ??0.005 ??0.45 |
| ??2??DO ??NO 2- ??NH 3-N | ??8.0-9.0 ??0.15 ??1.45 | ??9.0-10.0 ??0.15 ??1.45 | ??10-11 ??0.15 ??1.42 | ??11-12 ??0.10 ??1.20 | ??13-14 ??0.10 ??1.10 | ??13-14 ??0.015 ??0.80 | ??13-14 ??0.010 ??0.55 | ??13-14 ??0.005 ??0.45 |
| 3 contrast DO NO 2- ??NH 3-N | ??8.0-9.0 ??0.15 ??1.45 | ??8.0-9.0 ??0.15 ??1.45 | ??8.0-9.0 ??0.15 ??1.45 | ??8.0-9.0 ??0.15 ??1.43 | ??8.0-9.0 ??0.15 ??1.40 | ??9.0-10.0 ??0.15 ??1.30 | ??9.0-10.0 ??0.15 ??1.20 | ??9.0-10.0 ??0.15 ??1.10 |
| ??4???DO ??NO 2- ??NH 3-N | ??8.0-9.0 ??0.15 ??1.43 | ??9.0-10.0 ??0.15 ??1.43 | ??10-11 ??0.15 ??1.40 | ??11-12 ??0.10 ??1.30 | ??13-14 ??0.10 ??1.20 | ??13-14 ??0.015 ??0.90 | ??13-14 ??0.010 ??0.55 | ??13-14 ??0.005 ??0.50 |
| ??5???DO ??NO 2- ??NH 3-N | ??8.0-9.0 ??0.15 ??1.45 | ?9.0-10.0 ?0.15 ?1.45 | ?10-11 ?0.15 ?1.42 | ?11-12 ?0.10 ?1.30 | ?13-14 ?0.10 ?1.20 | ?13-14 ?0.015 ?0.90 | ?13-14 ?0.010 ?0.60 | ?13-14 ?0.005 ?0.55 |
| 6 contrast DO NO 2- ??NH 3-N | ??8?0-9.0 ??0.15 ??1.45 | ?8.0-9.0 ?0.15 ?1.45 | ?8.0-9.0 ?0.15 ?1.45 | ?8.0-9.0 ?0.15 ?1.43 | ?8.0-9.0 ?0.15 ?1.40 | ?9.0-10.0 ?0.15 ?1.30 | ?9.0-10.0 ?0.15 ?1.20 | ?9.0-10.0 ?0.15 ?1.10 |
Water sampling during 15:00 in the afternoon, adopt the water quality real-time analysis box of department of chemistry of Peking University development to measure dissolved oxygen (DO value), nitrite, ammonia-nitrogen content, the results are shown in Table 4, table 5. as can be seen from Table 4, the DO value is from between 0d～7d, 1, preparation is used in 2,3, No. 4 ponds, the big breeding of algae is given birth to high gradually at the oxygen that increases and produce, all at the 4d value of reaching capacity 13-14mg/L; Contain nitrite ion in the pond waters and in 0d～7d, descend fast, all reach 0.005mg/L, the result of a large amount of beneficial bacteria propagation back effects in the preparation is described, and 3, No. 6 pond control group nitrite ion content change not from 0.15mg/L; In the pond owing to used ammonium hydrogencarbonate, NH
3-N content is very high, and 3, No. 6 ponds of control group do not take place significantly to reduce, and after 1,2,4, No. 5 pond uses preparation, because useful algae and beneficial bacteria are bred in a large number and have been utilized ammonia nitrogen, from 4d～7d, water body content and control group significantly reduce, and are remarkable difference p＜0.01.
Table 5 uses preparation pond dissolved oxygen, nitrite, ammonia-nitrogen content in 0-180d to change
| The pond numbering | ??20d ??mg/L | ??40d ??mg/L | ??60d ??mg/L | ??80d ??mg/L | ??100d ??mg/L | ??120d ??mg/L | ??140d ??mg/L | ??180d ??mg/L |
| ??1??DO ??NO 2- ??NH 3-N | ??12.0-13.0 ??0.005 ??0.10 | ??12.0-13.0 ??0.005 ??0.10 | ??11.0-12.0 ??0.005 ??0.07 | ??10.0-11.0 ??0.015 ??0.06 | ??10.0-11.0 ??0.015 ??0.09 | ??9.0-10.0 ??0.020 ??0.08 | ??9.0-10.0 ??0.025 ??0.08 | ??9.0-10.0 ??0.05 ??0.06 |
| ??2??DO ??NO 2- ??NH 3-N | ??12.0-13.0 ??0.005 ??0.09 | ??12.0-13.0 ??0.005 ??0.10 | ??11.0-12.0 ??0.005 ??0.06 | ??10.0-11.0 ??0.015 ??0.09 | ??10.0-11.0 ??0.015 ??0.09 | ??9.0-10. ??0.020 ??0.07 | ??9.0-10.0 ??0.025 ??0.05 | ??9.0-10.0 ??0.05 ??0.07 |
| 3 contrast DO NO 2- ??NH 3-N | ??7.0-8.0 ??0.16 ??0.68 | ??7.0-8.0 ??0.17 ??0.64 | ??6.0-7.0 ??0.20 ??0.69 | ??6.0-7.0 ??0.23 ??0.67 | ??7.0-8.0 ??0.26 ??0.56 | ??6.0-7.0 ??0.26 ??0.79 | ??6.0-7.0 ??0.28 ??0.80 | ??6.0-7.0 ??0.33 ??0.81 |
| ??4???DO ??NO 2- ??NH 3-N | ??12.0-13.0 ??0.010 ??0.10 | ??12.0-13.0 ??0.015 ??0.15 | ??11.0-12.0 ??0.035 ??0.09 | ??10.0-11.0 ??0.035 ??0.08 | ??9.0-10.0 ??0.055 ??0.10 | ??9.0-10.0 ??0.050 ??0.13 | ??8.0-9.0 ??0.056 ??0.09 | ??7.0-8.0 ??0.058 ??0.10 |
| ??5???DO ??NO 2- ??NH 3-N | ??12.0-13.0 ??0.010 ??0.10 | ??12.0-13.0 ??0.015 ??0.15 | ??11.0-12.0 ??0.015 ??0.07 | ?10.0-11.0 ?0.030 ?0.08 | ??9.0-10.0 ??0.035 ??0.09 | ??9.0-10.0 ??0.045 ??0.11 | ??8.0-9.0 ??0.055 ??0.09 | ??7.0-8.0 ??0.065 ??0.08 |
| 6 contrast DO NO 2- ??NH 3-N | ??7.0-8.0 ??0.16 ??0.68 | ??7.0-8.0 ??0.17 ??0.64 | ??6.0-7.0 ??0.20 ??0.69 | ?6.0-7.0 ?0.23 ?0.67 | ??7.0-8.0 ??0.26 ??0.56 | ??6.0-7.0 ??0.26 ??0.79 | ??6.0-7.0 ??0.28 ??0.80 | ??6.0-7.0 ??0.33 ??0.81 |
Table 5 as can be seen, produce period at 20d-180d, 1,2,4, No. 5 each pond nitrite, ammonia-nitrogen contents significantly do not raise, main improver of water quality has been brought into play effect, and No. 3 ponds of control group are fingerlings though culture, preliminary stage does not use mixed feed, improve not quite, but behind the later stage use mixed feed, all obviously raise; No. 6 ponds particularly, the feed of always throwing something and feeding, so nitrite, ammonia nitrogen significantly raise, dissolved oxygen is in lower level.So improver of water quality of the present invention can significantly decompose nitrite, ammonia nitrogen.
2.4 the inspection of bottom water bacteria quantity
To each pond bottom hydromining sample, check that bacterial species, quantity are shown in Table 6.After preparation is invested in the cultivating pool, beneficial microorganism can be bred rapidly becomes dominant microflora, mainly be to decompose nitrite, ammonia nitrogen, in addition, after a large amount of probiotics breedings, competition that can be by food, place and secrete similar antibiotic material suppresses harmful pathogen growth breeding directly or indirectly.
Every 20d bacterial species in table 6 pond and quantity (10
6CFU/mL)
| The pond numbering | Plant plankton biomass mg/L transparency cm | |||||||
| 20d | 40d | 80d | 100d | 120d | 140d | 160d | 180d | |
| 1 total bacterial nitrification bacterium nitrite bacteria yeast | ????30 ????12 ????10 ????8 | ????35 ????10 ????16 ????7 | ????38 ????13 ????12 ????13 | ????32 ????10 ????10 ????11 | ????37 ????12 ????9 ????14 | ????43 ????9 ????16 ????13 | ????35 ????8 ????16 ????9 | ????38 ????13 ????12 ????8 |
| 2 total bacterial nitrification bacterium nitrite bacteria yeast | ????33 ????7 ????5 ????10 | ????38 ????8 ????7 ????11 | ????39 ????12 ????10 ????10 | ????35 ????14 ????9 ????6 | ????33 ????10 ????7 ????10 | ????38 ????9 ????11 ????13 | ????35 ????10 ????7 ????6 | ????35 ????8 ????12 ????5 |
| 3 total bacterial nitrification bacterium nitrite bacteria yeast | ????22 ????0.4 ????0.6 ????0.01 | ????24 ????0.8 ????0.6 ????0.03 | ????20 ????0.5 ????0.4 ????0.05 | ????16 ????0.4 ????0.2 ????0.07 | ????18 ????0.7 ????0.7 ????0.08 | ????17 ????0.9 ????0.5 ????0.09 | ????16 ????0.5 ????0.6 ????0.04 | ????17 ????0.8 ????0.07 ????0.05 |
2.5 fingerling, adult fish growth performance and incidence
In the nurture of fish fingerlings process, 1, No. 2 sterilizing agent is never used in the test pond, uses copper sulfate and Trichlorphon desinsection mid-term each 1 time, other disease does not take place in the production take place; 4, No. 5 pond medication number of times are total 3 times, and 1 time is sterilant Trichlorphon, and 2 times is dioxide peroxide, 1 rotted gill disease of production period outburst, 1 doctylogyrosis; No. 3 bacteremic septicemia takes place in the contrast pond in August, No. 6 contrast pond morbidities 3 times, 1 rotted gill disease, 1 bacteremic septicemia, 1 enteritis, in the production, every 20d uses 1 sterilizing agent, uses altogether 5 times, mainly be to use said preparation after degraded enter the various organism of culturing the pond, a large amount of breeding growths of unicellular algae and beneficial bacteria have suppressed the breeding of pathogenic bacteria; The propagation of algae increases dissolved oxygen effectively simultaneously, eliminates the poisonous factor, has built good ecotope.Cultivated animals is grown in the loose environment, it is active to ingest, fast growth, particularly fry is in nurture of fish fingerlings, algae proliferation in the preparation is to producing the very big effect that produces, reduced the amount of using feed in producing, also reduced production cost (being shown in Table 7), 1,2, No. 3 pond feeding costs are with under identical, 1, No. 2 pond test group per mu yields and No. 3 ponds of control group on average exceed 108.5kg, mean body weight surpasses 27g, test group and control group all show significant difference p＜0.01, and be same, and 4, No. 5 adult fish ponds and No. 6 pond control groups are relatively, mean body weight and output also show significant difference p＜0.01, and they are compared production cost and reduce with control group.The fish disease prevention and cure expense shows, test group is than low many of control group expense.
Each pond yields of table 7, upgrowth situation, feed cost, diseases prevention and treatment medicine expense
| The pond numbering | The average every mu of medicine of the average every mu of feeding cost of per mu yield (kg) mean body weight (g) (unit) takes (unit) |
| 123 contrasts, 456 contrasts | ??564???????????????132.5????????????2600??????????????????????????????20 ??589???????????????125.1????????????2600??????????????????????????????20 ??468???????????????101.8????????????2600??????????????????????????????70 ??846???????????????865.2????????????2500??????????????????????????????50 ??881???????????????879.6????????????2500??????????????????????????????50 ??795???????????????769.5????????????2500??????????????????????????????130 |
3. conclusion
In a word, use ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent of the present invention, can purify water, increase the dissolved oxygen of water body, reduce the ammonia-state nitrogen (NH in the water body
3-N) and nitrite nitrogen (NO
2 -) etc.; Simultaneously, provide the aquatic animal natural bait, improve the water body production performance; The breeding and the growth of control harmful algae, mushroom, the propagation of probiotics has also limited the breeding quantity of aquatic pathogenic bacteria, plays the purpose of ecological diseases prevention, so said preparation has wide application value.
Claims (3)
1. a ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent is characterized in that, contains following quality than raw material:
1) cell quantity 〉=10
20The algae concentrated solution of CFU/ml, 30%～85%;
2) cell quantity 〉=10
40The bacterium concentrated solution of CFU/ml, 10%～60%;
3) phycomycete special nutrient fluid, 4%～20%;
4) special-purpose suspension agent, 0.10%～0.25% preserved;
The summation of above-mentioned raw materials is 100%;
Above-mentioned algae concentrated solution is selected from the compound algae of green alga or diatom or the two;
Described bacterium concentrated solution is the concentrated solution that vinelandii, X401 aerogen, nitrobacteria, nitrite bacteria, yeast are mixed with;
Described special-purpose preservation suspension agent is agar powder, dextran, Sargassum polysaccharides mixture, and ratio is 12: 5: 1;
Described phycomycete special nutrient fluid contains following ingredients and mixes with 1000ml water:
NH
4CI????????????????????????????????????0.21g
Ca(H
2PO
2)
2·H
2O+2CaSO
2·H
2O???????0.03g
MgSO
4·7H
2O?????????????????????????????0.07g
NaHCO
3???????????????????????????????????0.11g
KCI???????????????????????????????????????0.03g
Fish meal 0.5g～2.0g.
2. ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent as claimed in claim 1, it is characterized in that, the prescription of the concentrated solution that described vinelandii, X401 aerogen, nitrobacteria, nitrite bacteria, yeast are mixed with is: X401 aerogen 30%, vinelandii 20%, yeast 10%, nitrobacteria 20%, nitrite bacteria 20%.
3. realize the preparation method of the ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent of claim 1, it is characterized in that, undertaken by following step successively:
The cultivation of a, algae
Indoor in algae culture, after the green alga of pure culture or diatom cultivated through one-level, secondary, be inoculated into and inflate cultivation in tun or the cement pit in a large number, nutrient solution is through disinfecting, with grid algae, green alga is the algae source, adopt the x06 nutrient solution to cultivate, the x06 nutrient solution contains following ingredients and mixes with 1000ml water:
Urea 0.133g
Ca(H
2PO
4)
2·H
2O?????????????????????0.020g
Phosphoric acid 0.020ml
MgSO
4·7H
2O????????????????????????????0.10g
NaHCO
3??????????????????????????????????0.10g
KCl??????????????????????????????????????0.033g
The FeSO of 1% aqueous solution
40.20ml
The calcium chloride 0.05ml of 5% aqueous solution
0.05% fish meal leach liquor 0.5ml;
Adopt continuous culture method, cultivated 3 days down, shake every day bottle 4-6 time intensity of illumination 5600Lx, 25 ℃, when cell quantity above 10
6Obtain algae culture liquid during CFU/ml;
The cultivation of b, bacterium
Respectively X401 aerogen, vinelandii, yeast, nitrobacteria, nitrite bacteria are cultivated through one-level, secondary, the ordinary broth nutrient solution that is inoculated into the bacterium of going out in tun or the cement pit is more respectively inflated cultivation in a large number, cultured continuously 48～72 hours is when bacterial count 〉=10
20Behind the CFU/ml, obtain inoculum;
C, algae, inoculum concentrate
Add the flocculation agent tschermigite respectively in algae, inoculum, making concentration is 0.05%, stirs 30 minutes, after the standing over night, removes supernatant liquor, cultivates respectively 1-2 days again, puts super-magnum centrifuge centrifugal concentrated thick algae, bacterium enriched material;
D, mixing concentrate the preparation of bacterium liquid
After the X401 aerogen cultivated respectively, vinelandii, yeast, nitrobacteria, nitrite bacteria flocculation, centrifugal concentrating, mix to concentrate bacterium liquid by following prescription, its prescription is:
X401 aerogen 30%
Vinelandii 20%
Yeast 10%
Nitrobacteria 20%
Nitrite bacteria 20%
E, special-purpose preparation of preserving suspension agent
With agar powder, dextran, Sargassum polysaccharides mixture, its ratio is mixing in 12: 5: 1, be mixed by the described phycomycete special nutrient fluid of claim 1 mass ratio again, place the stainless steel jacketed kettle, 100 ℃ of temperature controls, heated and stirred 5-10 minute, be incubated then 45 ℃ down standby, promptly obtain the special-purpose suspension agent of preserving;
F, algae bacteria microecological equipment suspension agent preparation
With useful algae concentrated solutions such as green alga, diatoms, vinelandii, X401 aerogen, nitrobacteria, nitrite bacteria, yeast mix concentrated bacterium liquid, the special use of above-mentioned preparation is preserved three kinds of compositions of suspension and is mixed according to the described mass ratio of claim 1, heat 35 ℃-40 ℃, stirred 5 minutes with homogenizer, restir is 5 minutes after the cooling room temperature, promptly gets the ultra concentrated algae bacteria microecological equipment suspension type water quality modifying agent.
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| CNB2005100417754A CN1313395C (en) | 2005-03-09 | 2005-03-09 | Super-concentrated algae bacterium micro-ecological balance suspension type water quality modifier and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102381797A (en) * | 2011-10-14 | 2012-03-21 | 龙源海洋生物股份有限公司 | Method for treating waste liquid and waste gas produced by fish meal processing |
| CN102517237A (en) * | 2011-12-30 | 2012-06-27 | 湖南农业大学 | Composite micro-ecological water-based suspension concentrate for aquaculture |
| CN107475143A (en) * | 2016-06-08 | 2017-12-15 | 易力源（厦门）环保科技有限公司 | Microorganism consortium for wastewater treatment |
| CN110896916A (en) * | 2019-12-04 | 2020-03-24 | 范秀娟 | Method for controlling harmful algae stable ecosystem by aquaculture water |
| CN114885866A (en) * | 2022-04-20 | 2022-08-12 | 资中县枫盛科技有限公司 | Ecological fish culture method using composite beneficial algae |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101811775B (en) * | 2009-02-19 | 2012-01-25 | 中国环境科学研究院 | Composite bacteria-algae preparation method for purifying aquaculture waste water |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07132080A (en) * | 1993-11-10 | 1995-05-23 | Masakazu Ito | Cell composition |
| TW247871B (en) * | 1994-11-22 | 1995-05-21 | Sy-Wei Wang | Agent for microbiological water quality stabilization and purification used in aquaculture |
| NO310716B1 (en) * | 1999-11-26 | 2001-08-20 | Health By Nature As | Means for improving drinking water |
| CN1266054C (en) * | 2002-11-12 | 2006-07-26 | 上海创博生态工程有限公司 | Modifying agent for microorganism breeding water and preparing method thereof |
| US20050011464A1 (en) * | 2003-07-14 | 2005-01-20 | The Chinese University Of Hong Kong | Additive composition for feeding aquaculture animals and feed containing the same |
| DE202004013885U1 (en) * | 2004-09-04 | 2004-11-11 | Heissner Ag | Ecologically acceptable, water treatment composition, useful for improving water quality in ponds, lakes or swimming baths, containing e.g. bacteria, fungi, yeasts, minerals, vitamins and/or enzymes |
-
2005
- 2005-03-09 CN CNB2005100417754A patent/CN1313395C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102381797A (en) * | 2011-10-14 | 2012-03-21 | 龙源海洋生物股份有限公司 | Method for treating waste liquid and waste gas produced by fish meal processing |
| CN102517237A (en) * | 2011-12-30 | 2012-06-27 | 湖南农业大学 | Composite micro-ecological water-based suspension concentrate for aquaculture |
| CN107475143A (en) * | 2016-06-08 | 2017-12-15 | 易力源（厦门）环保科技有限公司 | Microorganism consortium for wastewater treatment |
| CN110896916A (en) * | 2019-12-04 | 2020-03-24 | 范秀娟 | Method for controlling harmful algae stable ecosystem by aquaculture water |
| CN110896916B (en) * | 2019-12-04 | 2022-05-03 | 范秀娟 | Method for controlling harmful algae stable ecosystem by aquaculture water |
| CN114885866A (en) * | 2022-04-20 | 2022-08-12 | 资中县枫盛科技有限公司 | Ecological fish culture method using composite beneficial algae |
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