CN1627055A - 高山冰缘植物抗冻蛋白分离与检测的方法及其装置 - Google Patents
高山冰缘植物抗冻蛋白分离与检测的方法及其装置 Download PDFInfo
- Publication number
- CN1627055A CN1627055A CN 200310121954 CN200310121954A CN1627055A CN 1627055 A CN1627055 A CN 1627055A CN 200310121954 CN200310121954 CN 200310121954 CN 200310121954 A CN200310121954 A CN 200310121954A CN 1627055 A CN1627055 A CN 1627055A
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- Prior art keywords
- antifreeze protein
- protein
- ice crystal
- liquid nitrogen
- freezing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 12
- 108010053481 Antifreeze Proteins Proteins 0.000 title claims description 32
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 38
- 241000196324 Embryophyta Species 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 238000001953 recrystallisation Methods 0.000 claims abstract description 19
- 239000013078 crystal Substances 0.000 claims abstract description 18
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 13
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 13
- 239000000843 powder Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 239000000287 crude extract Substances 0.000 claims description 12
- 238000007710 freezing Methods 0.000 claims description 12
- 230000008014 freezing Effects 0.000 claims description 12
- 239000000523 sample Substances 0.000 claims description 11
- 238000000926 separation method Methods 0.000 claims description 8
- 238000010612 desalination reaction Methods 0.000 claims description 7
- URLKBWYHVLBVBO-UHFFFAOYSA-N Para-Xylene Chemical group CC1=CC=C(C)C=C1 URLKBWYHVLBVBO-UHFFFAOYSA-N 0.000 claims description 6
- 238000010521 absorption reaction Methods 0.000 claims description 6
- 239000003463 adsorbent Substances 0.000 claims description 6
- 239000006059 cover glass Substances 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 6
- 239000008363 phosphate buffer Substances 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- YVNQAIFQFWTPLQ-UHFFFAOYSA-O [4-[[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylidene]-3-methylcyclohexa-2,5-dien-1-ylidene]-ethyl-[(3-sulfophenyl)methyl]azanium Chemical compound C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C(=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C)C=2C(=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C)C=C1 YVNQAIFQFWTPLQ-UHFFFAOYSA-O 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 235000003351 Brassica cretica Nutrition 0.000 claims description 4
- 235000003343 Brassica rupestris Nutrition 0.000 claims description 4
- 229920002594 Polyethylene Glycol 8000 Polymers 0.000 claims description 4
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 claims description 4
- 150000002500 ions Chemical class 0.000 claims description 4
- 235000010460 mustard Nutrition 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 244000292693 Poa annua Species 0.000 claims description 3
- 244000292697 Polygonum aviculare Species 0.000 claims description 3
- 235000006386 Polygonum aviculare Nutrition 0.000 claims description 3
- 241001165494 Rhodiola Species 0.000 claims description 3
- PPCUPSGMDOTIMV-UHFFFAOYSA-N Sachalin Natural products CC1=C/C2OC(=O)C(=C)C2C(O)CC(=C)C(O)CC1 PPCUPSGMDOTIMV-UHFFFAOYSA-N 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- 238000005138 cryopreservation Methods 0.000 claims description 2
- 229910001873 dinitrogen Inorganic materials 0.000 claims description 2
- 230000003287 optical effect Effects 0.000 claims description 2
- 235000018102 proteins Nutrition 0.000 claims 2
- 244000056139 Brassica cretica Species 0.000 claims 1
- 238000007654 immersion Methods 0.000 claims 1
- 235000004252 protein component Nutrition 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- 102000040739 Secretory proteins Human genes 0.000 abstract 1
- 108091058545 Secretory proteins Proteins 0.000 abstract 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 1
- 239000000872 buffer Substances 0.000 abstract 1
- 238000009795 derivation Methods 0.000 abstract 1
- 238000000502 dialysis Methods 0.000 abstract 1
- 238000004161 plant tissue culture Methods 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 239000002984 plastic foam Substances 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241000219198 Brassica Species 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000013019 agitation Methods 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000002135 phase contrast microscopy Methods 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000009777 vacuum freeze-drying Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000209082 Lolium Species 0.000 description 1
- 244000228295 Polygonum viviparum Species 0.000 description 1
- 235000012804 Polygonum viviparum Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
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Images
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- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200310121954 CN1284796C (zh) | 2003-12-08 | 2003-12-08 | 高山冰缘植物抗冻蛋白分离与检测的方法及其装置 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200310121954 CN1284796C (zh) | 2003-12-08 | 2003-12-08 | 高山冰缘植物抗冻蛋白分离与检测的方法及其装置 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1627055A true CN1627055A (zh) | 2005-06-15 |
CN1284796C CN1284796C (zh) | 2006-11-15 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 200310121954 Expired - Fee Related CN1284796C (zh) | 2003-12-08 | 2003-12-08 | 高山冰缘植物抗冻蛋白分离与检测的方法及其装置 |
Country Status (1)
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CN (1) | CN1284796C (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106883293A (zh) * | 2017-04-26 | 2017-06-23 | 昆明学院 | 一种快速分离抗冻蛋白的方法 |
CN111795909A (zh) * | 2019-04-09 | 2020-10-20 | 中国科学院化学研究所 | 一种筛选控冰材料的方法 |
CN114689874A (zh) * | 2022-06-01 | 2022-07-01 | 深圳市帝迈生物技术有限公司 | 用于液体稳定和抗冻融的d-二聚体试剂 |
-
2003
- 2003-12-08 CN CN 200310121954 patent/CN1284796C/zh not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106883293A (zh) * | 2017-04-26 | 2017-06-23 | 昆明学院 | 一种快速分离抗冻蛋白的方法 |
CN111795909A (zh) * | 2019-04-09 | 2020-10-20 | 中国科学院化学研究所 | 一种筛选控冰材料的方法 |
CN111795909B (zh) * | 2019-04-09 | 2022-04-08 | 中国科学院化学研究所 | 一种筛选控冰材料的方法 |
CN114689874A (zh) * | 2022-06-01 | 2022-07-01 | 深圳市帝迈生物技术有限公司 | 用于液体稳定和抗冻融的d-二聚体试剂 |
Also Published As
Publication number | Publication date |
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CN1284796C (zh) | 2006-11-15 |
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Date | Code | Title | Description |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: LANZHOU UNIV.; AN LIZHE Free format text: FORMER OWNER: AN LIZHE Effective date: 20080307 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20080307 Address after: 222, Tianshui South Road, Lanzhou, Gansu, zip code: 730000 Co-patentee after: An Lizhe Patentee after: Lanzhou University Address before: Lanzhou University, Lanzhou, Gansu, China: 730000 Patentee before: An Lizhe |
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C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20061115 Termination date: 20100108 |