CN1586185A - Method and device for catfish artificial propogation and fertilized egg circular path hatching - Google Patents
Method and device for catfish artificial propogation and fertilized egg circular path hatching Download PDFInfo
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- CN1586185A CN1586185A CNA2004100722911A CN200410072291A CN1586185A CN 1586185 A CN1586185 A CN 1586185A CN A2004100722911 A CNA2004100722911 A CN A2004100722911A CN 200410072291 A CN200410072291 A CN 200410072291A CN 1586185 A CN1586185 A CN 1586185A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention relates to method and apparatus for artificial catfish breeding and fertilized egg hatching in circular path. Sexually mature female and male catfish over 3 ages are injected with foreign hormone to induce ovulation and spermiation for artificial fertilization, and the fertilized egg is suspended in flowing water with dissolved oxygen over 6 mg/L inside a circular path for hatching. The hatching apparatus has circular or elliptic pond with circular path. The present invention can realize the large scale production of catfish fly and each of the circular path can hatch fertilized eggs over 10 million in the fertilization rate and hatching rate over 95 %.
Description
Technical field
The invention belongs to method for cultivation of fish, particularly relate to a kind of catfish artificial propagation and fertilized egg circuit hatching m and facility.
Background technology
Breeding present known catfish mainly relies on natural spawning, sometimes also be aided with the injection exogenous hormone, spawning pond is arranged bionical fish nest, allow sexual maturity or the catfish natural spawning of hastening parturition on the fish nest, collect the fish nest that is stained with fish-egg then, put it in the hatchery and hatch.Owing to be subjected to influencing parent population and can not concentrating and lay eggs of weather and natural conditions, also have some ovum to be embedded in the soil owing to there is weak body fish to lay eggs, so the natural spawning rate is only up to 40-50%; And the duration of laying eggs is very long, is difficult to implement the fry large-scale production, has hindered the development of catfish aquaculture.
Summary of the invention
The present invention can obtain well-developed catfish sperm and ovum in enormous quantities for the technical problem that exists in the solution known technology provides a kind of, and catfish artificial propagation and the fertilized egg circuit hatching m and the facility of hatching on a large scale.
The technical scheme that the present invention takes for the technical problem that exists in the solution known technology is:
1). selecting female, the milter of above sexual maturity in 3 ages, is luteinizing hormone releasing hormone A at spawning season with exogenous hormone
2(LHRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG) mix the back and become injection with dissolved in distilled water, induces its ovulation and row smart at its pectoral fin portion or lumbar injection;
For the above-mentioned injection drug dosage of per kilogram fish body weight be: LHRH-A
232 micrograms, 6.0 milligrams of DOM and HCG 2400IU (international unit); Injection at twice: be LHRH-A for the first time
28.0 microgram, 6.0 milligrams of DOM and 1 ml distilled water; Be LHRH-A for the second time
224 micrograms, HCG 2400IU and 1 ml distilled water; Water temperature 23-28 ℃, female, milter dosage is identical, the double injection time interval is 11 hours;
2). induce its ovulation, the smart parent population of row to hasten parturition the back under water temperature 23-26 ℃ condition above-mentioned, squeezed ovum through 8-10 hour again, squeeze smart and carry out the dry method artificial insemination, after this fertilized egg is put into the circuit in circuit pond again, fertilized egg is suspended in the mobile water body; Each circuit is sterilized and was changed water in back 30 minutes with 13g malachite green sterilization 2 times;
3). circuit pond flow rate of water flow will be controlled at fertilized egg is suspended in the water layer, and dissolved oxygen in water reaches more than 6 mg/litre.
A kind of catfish fertilized egg circuit hatching facility, it includes circular circuit pond or oval circuit pond, and the bolting silk enclosure wall that forms circuit with circuit pond outer wall is installed in the circuit pond, and makes the water pump that water circulation flows in the circuit.
Advantage and good effect that the present invention has are: induce the parent population ovulation for ripe strong catfish parent population injection exogenous hormone owing to adopt, can obtain well-developed sperm and ovum, again by artificial insemination.And then the fertilized egg that obtains is put in the circuit in circuit pond and hatch.The present invention can concentrate the short time efficiently to lay eggs and hatch like this, thereby has realized the large-scale production of catfish fry.Adopt a circuit hatching of the present invention fertilized egg can reach more than 1,000 ten thousand, fertilization rate and incubation rate can reach more than 95% respectively.
Embodiment
For further understanding content of the present invention, characteristics and effect, exemplify following examples now and be described in detail as follows:
1. the ripe catfish parent population ovulation of exogenous hormone inductivity, smart artificial propagation and the fertilized egg circuit hatching m of row:
1). 5 the end of month or June 23 from 3 age parent populations, select gonad development good, body weight is the raun more than 1 kilogram, the parent population of hastening parturition of the milter more than 0.6 kilogram.
2). induce parent population ovulation, the smart medicine of row to adopt luteinizing hormone releasing hormone A
2(LHRH-A
2), the mixture of maleic acid DOM (DOM) and 3 kinds of oxytocic drugs of human chorionic gonadtropin (HCG).The dosage per kilogram fish body weight of 3 kinds of medicines is used respectively: A
2(LHRH-A
2) 32 micrograms, 6.0 milligrams of maleic acid DOMs (DOM) and human chorionic gonadtropin (HCG) 2400 IU international units, these 3 kinds of medicines are mixed be dissolved in 23 ℃ or the 28 ℃ of distilled water as the injection injection, female, the milter drug dose is identical.Also above-mentioned 3 kinds of medicines can be mixed and be dissolved in 25 ℃ of distilled water as the injection injection.
3). oxytocic drug adopts 2 injections, and injected dose is with LHRH-A for per kilogram fish body weight for the first time
28.0 6.0 milligrams of mixing of microgram and DOM are dissolved in 1 milliliter 23 ℃ or the 28 ℃ of distilled water, also these 2 kinds of medicines can be mixed be dissolved in 25 ℃ of distilled water as the injection injection, and be that 6-7 point is injected in the afternoon; Injected dose is that per kilogram fish body weight is with LHRH-A for the second time
2The medicine of 24 micrograms and HCG 2400IU international unit mixes and also is dissolved in 1 milliliter 23 ℃ or the 28 ℃ of distilled water, also can above-mentioned 2 kinds of medicines mix be dissolved in 25 ℃ of distilled water as the injection injection, and in morning on next day the 6-7 point inject.The above-mentioned double injection time interval is 11 hours, and twice all is to induce its ovulation and row's essence in its pectoral fin portion or lumbar injection.
4). hasten parturition under 25 ℃ of conditions of water temperature, the effect time of hastening parturition was at 8,9 or 10 hours.The water temperature of hastening parturition also can be 23 ℃ or 26 ℃.
5). through adopting the dry method artificial insemination after the ecbolic effect time, to squeezing smart, crowded ovum through induced ovulation, the smart parent population of row, essence, ovum were stirred 10-20 minute gently, seminal fluid is evenly loose invest on each ovum, after 5-10 minute, the remaining seminal fluid of water flush away.
2. the artificial insemination ovum is put into circuit flowing water hatching, and fertilized egg is bonded to little particle or adheres on the bolting silk when just putting into; Soon viscosity diminishes, fertilized egg promptly is suspended in the mobile water body.
The circuit hatching will keep certain water level, and this water yield that just requires to enter in the circuit is consistent with the water yield of discharging circuit.The control of water yield size is to keep dissolved oxygen in water to reach more than 6 mg/litre.
The regulation and control of circuit flow rate of water flow are that fertilized egg is flowed with water, suspend in water layer, finish embryo development procedure, hatch fry at last.The regulation and control of current will be adjusted at any time with the growth of fertilized egg, make fertilized egg be suspended state all the time.
Fertilized egg sterilizes twice with malachite green after putting into the circuit pond, and each consumption is 13 grams, changes water after 30 minutes.
In addition, also to scrub and observe the process of development of fertilized ova in the circuit to the bolting silk net.
Catfish artificial propagation and fertilized egg circuit hatching facility comprise: circuit pond, bolting silk enclosure wall and water pump.
Circuit pond: two kinds in circular pond and oval pond arranged.13 meters of circular pond diameter about 8 meters or oval pond major diameters, 7 meters of minor axis, the circuit pond all becomes with brick or piece barnacle.
The screen cloth enclosure wall: at 7 meters of circuit internal diameter or 10 meters of major diameters, 5 meters of minor axis, the enclosure wall that a timber surrounds is set, external application 60 mesh sieve thin,tough silk tightly seal, and drain to outside the circuit not make the fertilized egg of flowing in the circuit.About 0.85 meter of distance between circuit cement plaster and the bolting silk enclosure wall, circuit depth of water 1-1.2 rice, this is the place that fertilized egg flowing hatched.Be provided with the oblique duck-beak type water pipe of direction unanimity at the bottom of the pond, Chi Shui is flowed to a direction, and make the fertilized egg of sinking at the bottom of the pond blow to the top layer,, cause anoxic to prevent fertilized egg local accumulation or deposition bottom.The outer place of inner ring of circuit in the circuit pond for keeping circuit water level and hatching water filtration to discharge, in be provided with two water pumps and make that water circulation flows in the circuit.
Claims (2)
1. catfish artificial fecundation method is characterized in that:
1). selecting female, the milter of above sexual maturity in 3 ages, is luteinizing hormone releasing hormone A at spawning season with exogenous hormone
2(LHRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG) mix the back and become injection with dissolved in distilled water, induces its ovulation and row smart at its pectoral fin portion or lumbar injection;
For the above-mentioned injection drug dosage of per kilogram fish body weight be: LHRH-A
232 micrograms, 6.0 milligrams of DOM and HCG 2400 IU (international unit); Injection at twice: be LHRH-A for the first time
28.0 microgram, 6.0 milligrams of DOM and 1 ml distilled water; Be LHRH-A for the second time
224 micrograms, HCG 2400 IU and 1 ml distilled water; Water temperature 23-28 ℃, female, milter dosage is identical, the double injection time interval is 11 hours;
2). induce its ovulation, the smart parent population of row to hasten parturition the back under water temperature 23-26 ℃ condition above-mentioned, squeezed ovum through 8-10 hour again, squeeze smart and carry out the dry method artificial insemination, after this fertilized egg is put into the circuit in circuit pond again, fertilized egg is suspended in the mobile water body; Each circuit is sterilized and was changed water in back 30 minutes with 13g malachite green sterilization 2 times;
3). circuit pond flow rate of water flow will be controlled at fertilized egg is suspended in the water layer, and dissolved oxygen in water reaches more than 6 mg/litre.
2. a catfish fertilized egg circuit hatching facility is characterized in that including circular circuit pond or oval circuit pond, the bolting silk enclosure wall that forms circuit with circuit pond outer wall is installed in the circuit pond, and makes the water pump that water circulation flows in the circuit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100722911A CN1283142C (en) | 2004-09-30 | 2004-09-30 | Method and device for catfish artificial propogation and fertilized egg circular path hatching |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100722911A CN1283142C (en) | 2004-09-30 | 2004-09-30 | Method and device for catfish artificial propogation and fertilized egg circular path hatching |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1586185A true CN1586185A (en) | 2005-03-02 |
| CN1283142C CN1283142C (en) | 2006-11-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100722911A Expired - Fee Related CN1283142C (en) | 2004-09-30 | 2004-09-30 | Method and device for catfish artificial propogation and fertilized egg circular path hatching |
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| CN (1) | CN1283142C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100431408C (en) * | 2006-06-29 | 2008-11-12 | 天津市换新水产良种场 | Method for breeding two hybrid species of fish |
| CN100486427C (en) * | 2006-10-13 | 2009-05-13 | 四川省农业科学院水产研究所 | Early breeding cultivating method of silurus meridionalis chen parent fish |
| CN102124975A (en) * | 2011-03-23 | 2011-07-20 | 中国水产科学研究院长江水产研究所 | Method for breeding tenuaeosa reevesii recruits in circuit running water way |
| CN102228681A (en) * | 2011-06-09 | 2011-11-02 | 吴江市水产养殖有限公司 | Fish spawning inducer and spawning induction method |
| CN103583435A (en) * | 2013-11-13 | 2014-02-19 | 叶大铿 | Eel leucomalachite-green-removal aquaculture method |
| CN104365513A (en) * | 2014-10-31 | 2015-02-25 | 安徽华亿农牧科技发展有限公司 | Catfish in-genera cross breeding method |
| CN106489801A (en) * | 2016-12-12 | 2017-03-15 | 福建省淡水水产研究所 | A kind of half pierces thick lip fish artificial seedling rearing's method |
-
2004
- 2004-09-30 CN CNB2004100722911A patent/CN1283142C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100431408C (en) * | 2006-06-29 | 2008-11-12 | 天津市换新水产良种场 | Method for breeding two hybrid species of fish |
| CN100486427C (en) * | 2006-10-13 | 2009-05-13 | 四川省农业科学院水产研究所 | Early breeding cultivating method of silurus meridionalis chen parent fish |
| CN102124975A (en) * | 2011-03-23 | 2011-07-20 | 中国水产科学研究院长江水产研究所 | Method for breeding tenuaeosa reevesii recruits in circuit running water way |
| CN102228681A (en) * | 2011-06-09 | 2011-11-02 | 吴江市水产养殖有限公司 | Fish spawning inducer and spawning induction method |
| CN103583435A (en) * | 2013-11-13 | 2014-02-19 | 叶大铿 | Eel leucomalachite-green-removal aquaculture method |
| CN104365513A (en) * | 2014-10-31 | 2015-02-25 | 安徽华亿农牧科技发展有限公司 | Catfish in-genera cross breeding method |
| CN104365513B (en) * | 2014-10-31 | 2016-08-17 | 安徽华亿农牧科技发展有限公司 | A kind of Silurus asotus fish genus cross breeding method |
| CN106489801A (en) * | 2016-12-12 | 2017-03-15 | 福建省淡水水产研究所 | A kind of half pierces thick lip fish artificial seedling rearing's method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1283142C (en) | 2006-11-08 |
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