CN1425317A - Multiple microorganism straw fermented fodder and leaven and its preparing method - Google Patents

Abstract

The microbe fermented feed is produced through preparing fermenting agent with several kinds of microbial strains, mixing supplementary material, straw and activated fermenting agent, fermentation inside one container of fermenting pond. The fermenting agent has wide application range of fermentation conditions and various straw and vine, green or dry, of different crops may be used as the main material for producing the fermented feed. The fermented feed has good feeding effect, and is delicious and easy to digest and absorb.

Description

Multiple microbial straw fermented feed and leavening and preparation method thereof

Technical field

The present invention relates to a kind of animal feed, relate in particular to one kind of multiple microbial straw fermented feeds and leavening and preparation method thereof.

Background technology

At present, the technology that stalk is converted into feed has much both at home and abroad, mainly contains following several method:

Physical method: with crushed stalk, immersion, boiling, expanded or ray decomposition method.Simple use the physics method cellulosic material can't be transformed to reduce crude fiber content, so the palatability of feed can't effectively improve, be of low nutritive value.

Chemical method: alkalization straw feed, ammoniated straw feed, acid treatment straw feed.But it is serious that soda acid is handled contaminated wastewater, and the ammoniated straw feed cost is high and have the danger of ammonia poisoning.

The ensiling stalks feed: the crop plant of harvesting is piled up anaerobic fermentation when water content 60% is above, utilizes the natural microbial growth that is had in planting, make stalk fibre softening, change the palatability of stalk, but to the not raising of nutritional labeling of stalk.

Microbial process: microbiological anaerobic fermentation or oxygen consumption fermentation process are handled stalk and are converted into feed, the used mild condition environmentally safe of this method, cost is low, has improved the nutritive value and the digestibility of feed simultaneously, has improved the palatability of feed fully.Microbial process is a kind of effective stalk feed conversion method, but the bacterial classification of selecting for use is single, and mode is loaded down with trivial details, is primarily aimed at maize straw, and the feed of being produced is only as the ox feed, can not raise as other, the feed of bird.

Summary of the invention

Main purpose of the present invention is to solve the problem in the above-mentioned technology that stalk is converted into feed, provide the multiple microorganism of a kind of employing to cooperate fermentation Different Crop stalk to make feed technology, the Different Crop of can fermenting stalk (corn, wheat, paddy rice, Chinese sorghum and peanut seedling, sweet potato seedling etc.), and can produce multiple animal and fowl fodder (ox, sheep, pig, chicken, goose), particularly make stalk be converted into the feed of non-ruminant animal, and have and strengthen the disease resistance of livestock and poultry effect, leavening is strong to the adaptability of fermentation condition, the multiple microbial straw fermented feed that the feed safety that leavening is made is nontoxic and leavening and preparation method thereof.

The technical solution adopted for the present invention to solve the technical problems is:

The present invention uses many bacterial strains to cooperate anaerobic fermentation feed method, the leavening Different Crop stalk (corn, wheat, paddy rice, Chinese sorghum and peanut seedling, sweet potato seedling etc.) that can ferment, and can produce multiple poultry, fowl feed (ox, sheep, pig, chicken, goose), particularly make stalk be converted into the feed of non-ruminant animal, and have the effect that strengthens poultry, fowl premunition.

The present invention has the bacterial strain of cellulase-producing, hemicellulase, 1,4 beta-glucanase, protease, amylase, pectase, by the above-mentioned enzyme material of its growth secretion, with partial hydrolysis such as cellulose, hemicellulose, lignin in the stalk, improve the nutritive value of feed, and the digestive system that can enter into livestock is assisted the digestion of livestock endogenous enzymes, directly improved digesting and assimilating of feed, in addition, can effectively reduce the viscosity of enteron aisle Dissolve things inside, prevent to stop up the intestinal cell fenestra, promote the transmission and the absorption of nutriment, improve efficiency of feed utilization.

Have acid-producing bacteria, can produce the softening stalk of a large amount of organic acids and improve palatability, the stalk after the fermentation has strong sour fragrance, stimulates livestock appetite, increases feed intake.Acid can reduce pH value and suppress various spoilage organisms growths, prolongs the shelf-life of feed.The L-lactic acid that is produced is that the energy matter of ruminant directly improves its output of milk and weightening finish.Can stimulate digestion liquid secretion of lactic acid promotes gastrointestinal peristalsis, slows down gastrointestinal emptying speed, makes nutriment that digestion time more fully be arranged, and improves digestibility, and propionic acid can improve sugared content in the cow blood, reduces the toxic action of nuisance such as ketone to livestock.

Probio is added to pasture in the feed, can improves micro-ecological environment in the livestock body, suppress the growth of pathogenic bacteria in the livestock body, improve the resistance against diseases of livestock.Used bacterial classification sees the following form:

Table 1

Sequence number	Bacterium name (Chinese, Latin)	Numbering	Culture medium
				??1	Produce yellowish fiber monad Cellulomonas flanigena	??ACCC11055	Peptone 1%, glucose 0.5%, yeast extract 0.5%, PH7.0
??2	Bacillus amyloliquefaciens Bacillus amyloliquefaciens	??AS1.1226	Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, PH7.2-7.4
				??3	Bacillus coagulans Bacillus coagulans	??IFFI10067	Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, PH7.2-7.4
??4	Lactobacillus plantarum Lactobacillus plantarum	??AS1557	Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, PH7.2-7.4
				??5	Lactobacillus acidophilus Lactobacillus acidophilus	??IFFI06005	Skimmed milk power 10%, PH6.0
??6	Bafillus natto Bacillus natto	??IFFI10023	Peptone 1%, beef extract 0.5%, sodium chloride 0.5%, PH7.2-7.4
				??7	Propionibacterium freudenreichii Propionibacterium freudenreichii	??IFFI10019	Peptone 1%, sucrose 2%, yeast extract 1.0%, ammonium phosphate 0.5%, PH7.2-7.4
??8	Streptococcus fecalis Streptococcus faecalis	??CMCC65006	Peptone 1%, sucrose 2%, yeast extract 1.0%, ammonium phosphate 0.5%, PH7.2-7.4
				??9	Lactobacillus Lactobacillus sp	??IFFI06064	Skimmed milk power 10%, PH6.0
??10	Saccharomycete Saccharomyces sp	??AS2.1151	The YEPD culture medium
				??11	Candida utili Candida utilis	??AS2.120	The YEPD culture medium

Ruminant fermented feed leavening comprises that following component forms by weight ratio:

Produce yellowish fiber monad 0.5-1 part, bacillus amyloliquefaciens 0.5-1.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, propionibacterium freudenreichii 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, lactobacillus 0.5-1.5 part, saccharomycete 1-3 part.

Select some kinds of microorganism fungus kinds for use by proportioning, the technology of preparation ruminant fermented feed leavening is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification makes up in proportion → packs → the finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h.

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.5-1 part, bacillus amyloliquefaciens 0.5-1.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, propionibacterium freudenreichii 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, lactobacillus 0.5-1.5 part, saccharomycete .1-3 part is made up and is made leavening will to measure the bacterium number.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹¹Individual/g, content of ashes is less than 8%, and moisture is below 10%.

Non-ruminant animal fermented feed leavening comprises that following component forms by weight ratio:

Produce yellowish fiber monad 1.5-4.5 part, bacillus amyloliquefaciens 1.5-3.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, bafillus natto 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, candida utili 1-3 part.

Select some kinds of microorganism fungus kinds for use by proportioning, the technology of preparation non-ruminant animal fermented feed leavening is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification combination → packing → finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80～180 times/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33h.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 1.5-4.5 part, bacillus amyloliquefaciens 1.5-3.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, bafillus natto 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, candida utili 1-3 part is made up and is made leavening will to measure the bacterium number.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹³Individual/g, content of ashes is less than 8%, and moisture is below 10%.

Bird fermented feed leavening comprises that following component forms by weight ratio:

Produce yellowish fiber monad 1.5-4.5 part, bacillus amyloliquefaciens 0.5-2.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, bafillus natto 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, lactobacillus 0.5-2.5 part, saccharomycete 1-3 part.

Select some kinds of microorganism fungus kinds for use by proportioning, the technology of preparation bird fermented feed leavening is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification combination → packing → finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02～0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 1.5-4.5 part, bacillus amyloliquefaciens 0.5-2.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, bafillus natto 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, lactobacillus 0.5-2.5 part, saccharomycete 1-3 part is made up and is made leavening will to measure the bacterium number.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹³Individual/g, content of ashes is less than 8%, and moisture is below 10%.

The preparation method of ruminant fermented feed is as follows:

The leavening activation

↓

Crushed stalk → dressing and stalk mix → pack into container or pit entry fermentation (apparent temperature and decided 7-20 days) → finished product.

Dry straw is ground into 1-5 centimetre of section; With 0.1-0.5% syrup dissolving leavening, under 20-40 ℃ of state, to place and used later in 2-4 hour, the leavening after the activation used in 20 hours.

Dressing mixes with dry straw, and dry straw per ton adds dressing and the water 1-2t of sugar 0.1～1kg, corn 1-10kg, urea 1-5kg, salt 1-5kg, evenly admixes the leavening after the activation.With pack into container or go into the pond and ferment, essential compacting and secluding air, fermentation time 7-20 day of the stalk that has added leavening and dressing.

The finished product proterties: the color golden yellow has sour fragrance, the quality softness.

The fermented stalk feed proterties: digestibility improves 30-50% in the crude fibre degradation rate 10-17%, stalk body; Dry straw crude protein 3.2-4.5% before the fermentation, fermentation back crude protein content reaches 7.4-12%; Organic acid content reaches 2.0-1.6%; Moisture reaches 60-65%.

Various enzyme content: cellulase 100-112 unit/g, hemicellulase 15-20 unit/g, pectase 95-117 unit/g, proteinase 9 00-945 unit/g, glucan glycosides enzyme 80-87 unit/g.

The preparation method of Nonruminantia or bird fermented feed is as follows:

The leavening activation

↓

Crushed stalk → dressing and stalk mix → pack into container or pit entry fermentation (apparent temperature and decided 7-20 days) → finished product.

Dry straw is pulverized after 20 or 40 mesh sieves sieve; With 0.1-0.5% syrup dissolving leavening, under 20-40 ℃ of state, to place and used later in 2-4 hour, the leavening after the activation used up in 20 hours.

Dressing mixes with dry straw, and dry straw per ton adds dressing and the water 1-2t of sugar 0.1～1kg, corn 5-20kg, urea 1-10kg, salt 1-5kg, evenly admixes the leavening after the activation; With pack into container or go into the pond and ferment, essential compacting and secluding air, fermentation time 7-30 day of the stalk that has added leavening and dressing.

The finished product proterties: the color golden yellow has sour fragrance, the quality softness.

The fermented stalk feed proterties: digestibility improves 30-50% in the crude fibre degradation rate 10-17%, stalk body; Dry straw crude protein 3.2-4.5% before the fermentation, fermentation back crude protein content reaches 7.4-12%; Organic acid content reaches 2.0-1.6%; Moisture reaches 60-65%.

Various enzyme content: cellulase 100-112 unit/g, hemicellulase 15-20 unit/g, pectase 95-117 unit/g, proteinase 9 00-945 unit/g, glucan glycosides enzyme 80-87 unit/g.

By to control group and the experimental group nursing that experimentizes respectively of different poultrys, bird, replace the dry straw powder to experimentize with fermented stalk and feed.Feeding effect sees the following form:

Table 2 daily output (kilogram)

	Finished cattle	Milk cow	The little tail sheep that trembles with fear	Growing and fattening pigs	Laying hen	Open-minded goose (a jin/egg)
							Control group	??0.975	??0.975	??19.22	???0.166	(1.30 jin)	???14-15
Test group	??1.73	??1.73	??20.84	???0.23	(1.36 jin)	???8-10

The present invention meets standard GB/T6432-94 after testing; GB/T6433-94; GB/T6434-94; GB/T6436-92; GB/T6437-92; GB/T6438-92; GB/T6435-86; Wherein detect total moisture: 74.4%; Moisture: 7.02%: thick protein: 8.55%; Crude fat: 0.90%; Coarse ash: 13.28%; Total phosphorus: 0.19%; Calcium: 0.51%; Crude fibre; 25.12%.

The present invention is multiple microbial straw fermented feed and leavening and preparation method thereof.The feed safety that leavening is made is nontoxic, and leavening is strong to the adaptability of fermentation condition.0-35 ℃ can both ferment, and fermenting speed is directly proportional with ambient temperature, and 20 ℃ with top fermentation 7-10 days, 15 ℃ of fermentations 10-15 days.The applicable raw materials scope is wide, and ingredient requirement is extensive, and the various crop of can fermenting stalk comprises corn, wheat, paddy rice, broomcorn straw, barley culm, grain straw, oat straw, rotten straw; The stalk of legume: big beanstalk, broad bean straw, pea straw, and the rattan of crop, climing, seedling and peanut seedling, sweet potato seedling etc.And be applicable to blue or green stalk, double dry straw, dry straw and dry straw fermentation next year.Fermented stalk can be a kind of crop material various crop mixed straw that also can ferment.Fermented feed quality softness, loose, palatability is strong, and moisture reaches 60-65%.It is reasonable in design, simple to operate, easy to make to fill a prescription.Suitable wide farmers and breed factory only need hay cutter or pulverizer, reach rounds such as cylinder, bucket, polybag and can produce multiple microbial straw fermented feed.The nutritional labeling that fermented feed has improved stalk wherein crude fibre reduces 4-6%, and thick protein is brought up to 9-10%, and organic acid content reaches 1.6-2.0% and 70-80% is a L-lactic acid, can directly be absorbed by livestock.The feeding effect of fermented feed is fine.The feed palatability is better, and the digestibility of feed improves, and daily gain and meat feed ratio increase, and the livestock resistance against diseases increases.

The specific embodiment

Embodiment 1 (high metering)

Ruminant fermented feed leavening comprises that following component forms by every part of 0.235g weight proportion: produce yellowish fiber monad 0.235g, bacillus amyloliquefaciens 0.3525g, bacillus coagulans 0.3525g, Lactobacillus plantarum 0.3525g, propionibacterium freudenreichii 0.8225g, streptococcus fecalis 0.8225g, lactobacillus 0.3525g, saccharomycete 0.705g.

Embodiment 2 (low metering)

Ruminant fermented feed leavening comprises that following component forms by every part of 0.235g weight proportion:

Produce yellowish fiber monad 0.1175g, bacillus amyloliquefaciens 0.1175g, bacillus coagulans 0.1175g, Lactobacillus plantarum 0.1175g, propionibacterium freudenreichii 0.3525g, streptococcus fecalis 0.3525g, lactobacillus 0.1175g, saccharomycete 0.235g.

Embodiment 3 (medium metering)

Ruminant fermented feed leavening comprises that following component forms by every part of 0.235g weight proportion:

Produce yellowish fiber monad 0.17625g, bacillus amyloliquefaciens 0.235g, bacillus coagulans 0.235g, Lactobacillus plantarum 0.235g, propionibacterium freudenreichii 0.5875g, streptococcus fecalis 0.5875g, lactobacillus 0.235g, saccharomycete 0.47g.

Embodiment 4

Select 8 kinds of microorganism fungus kinds for use, promptly produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, Lactobacillus plantarum, propionibacterium freudenreichii, streptococcus fecalis, lactobacillus and saccharomycete, preparation technology is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification makes up in proportion → packs → the finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.235g, bacillus amyloliquefaciens 0.3525g, bacillus coagulans 0.3525g, Lactobacillus plantarum 0.3525g, propionibacterium freudenreichii 0.8225g, streptococcus fecalis 0.8225g, lactobacillus 0.3525g and the saccharomycete 0.705g that will measure the bacterium number makes up and makes leavening.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹¹Individual/gram, content of ashes is less than 8%, and moisture is below 10%.

Embodiment 5

Select 8 kinds of microorganism fungus kinds for use, promptly produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, Lactobacillus plantarum, propionibacterium freudenreichii, streptococcus fecalis, lactobacillus and saccharomycete, preparation technology is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification makes up in proportion → packs → the finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.1175g, bacillus amyloliquefaciens 0.1175g, bacillus coagulans 0.1175g, Lactobacillus plantarum 0.1175g, propionibacterium freudenreichii 0.3525g, streptococcus fecalis 0.3525g, lactobacillus 0.1175g and the saccharomycete 0.235g that will measure the bacterium number makes up and makes leavening.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹¹Individual/gram, content of ashes is less than 8%, and moisture is below 10%.

Embodiment 6

Select kind of a microorganism fungus kind for use, promptly produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, Lactobacillus plantarum, propionibacterium freudenreichii, streptococcus fecalis, lactobacillus and yeast, preparation technology is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification makes up in proportion → packs → the finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.17625g, bacillus amyloliquefaciens 0.235g, bacillus coagulans 0.235g, Lactobacillus plantarum 0.235g, propionibacterium freudenreichii 0.875g, streptococcus fecalis 0.875g, lactobacillus 0.235g and the saccharomycete 0.47g that will measure the bacterium number makes up and makes leavening.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹¹Individual/gram, content of ashes is less than 8%, and moisture is below 10%.

Embodiment 7 (high metering)

Non-ruminant animal fermented feed leavening comprises that following component forms by every part of 0.194g weight proportion:

Produce yellowish fiber monad 0.873g, bacillus amyloliquefaciens 0.679g, bacillus coagulans 0.291g, Lactobacillus plantarum 0.291g, lactobacillus acidophilus 0.291g, bafillus natto 0.679g, streptococcus fecalis 0.679g, candida utili 0.582g.

Embodiment 8 (low metering)

Non-ruminant animal fermented feed leavening comprises that following component forms by every part of 0.194g weight proportion:

Produce yellowish fiber monad 0.291g, bacillus amyloliquefaciens 0.291g, bacillus coagulans 0.097g, Lactobacillus plantarum 0.097g, lactobacillus acidophilus 0.097g, bafillus natto 0.291g, streptococcus fecalis 0.291g, candida utili 0.194g.

Embodiment 9 (medium metering)

Non-ruminant animal fermented feed leavening comprises that following component forms by every part of 0.194g weight proportion:

Produce yellowish fiber monad 0.582g, bacillus amyloliquefaciens 0.485g, bacillus coagulans 0.194g, Lactobacillus plantarum 0.194g, lactobacillus acidophilus 0.194g, bafillus natto 0.485g, streptococcus fecalis 0.485g, candida utili 0.388g.

Embodiment 10

Select 8 kinds of microorganism fungus kinds for use, promptly produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, Lactobacillus plantarum, lactobacillus acidophilus, bafillus natto, streptococcus fecalis and candida utili, preparation technology is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification combination → packing → finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature: 25-40 ℃, rotating speed 80～180 times/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature: 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.873g, bacillus amyloliquefaciens 0.679g, bacillus coagulans 0.291g, Lactobacillus plantarum 0.291g, lactobacillus acidophilus 0.291g, bafillus natto 0.679g, streptococcus fecalis 0.679g, candida utili 0.582g makes up and makes leavening will to measure the bacterium number.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹³Individual/g, content of ashes is less than 8%, and moisture is below 10%.

Embodiment 11

Select 8 kinds of microorganism fungus kinds for use, promptly produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, Lactobacillus plantarum, lactobacillus acidophilus, bafillus natto, streptococcus fecalis and candida utili, preparation technology is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification combination → packing → finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80～180 times/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.291g, bacillus amyloliquefaciens 0.291g, bacillus coagulans 0.097g, Lactobacillus plantarum 0.097g, lactobacillus acidophilus 0.097g, bafillus natto 0.291g, streptococcus fecalis 0.291g, candida utili 0.194g makes up and makes leavening will to measure the bacterium number.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹³Individual/g, content of ashes is less than 8%, and moisture is below 10%.

Embodiment 12

Select 8 kinds of microorganism fungus kinds for use, promptly produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, Lactobacillus plantarum, lactobacillus acidophilus, bafillus natto, streptococcus fecalis and candida utili, preparation technology is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification combination → packing → finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature: 25-40 ℃, rotating speed 80～180 times/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature: 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.582g, bacillus amyloliquefaciens 0.485g, bacillus coagulans 0.194g, Lactobacillus plantarum 0.194g, lactobacillus acidophilus 0.194g, bafillus natto 0.485g, streptococcus fecalis 0.485g, candida utili 0.388g makes up and makes leavening will to measure the bacterium number.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹³Individual/g, content of ashes is less than 8%, and moisture is below 10%.

Embodiment 13 (high metering)

Bird fermented feed leavening comprises that following component forms by every part of 0.188g weight proportion:

Produce yellowish fiber monad 0.846g, bacillus amyloliquefaciens 0.47g, bacillus coagulans 0.291g, Lactobacillus plantarum 0.291g, lactobacillus acidophilus 0.291g, bafillus natto 0.658g, streptococcus fecalis 0.658g, lactobacillus 0.47g, saccharomycete 0.564g.

Embodiment 14 (low metering)

Bird fermented feed leavening comprises that following component is by forming by every part of 0.188g weight proportion:

Produce yellowish fiber monad 0.291g, bacillus amyloliquefaciens 0.094g, bacillus coagulans 0.094g, Lactobacillus plantarum 0.094g, lactobacillus acidophilus 0.094g, bafillus natto 0.291g, streptococcus fecalis 0.291g, lactobacillus 0.094g, saccharomycete 0.188g.

Embodiment 15 (medium metering)

Bird fermented feed leavening comprises that following component is by forming by every part of 0.188g weight proportion:

Produce yellowish fiber monad 0.564g, bacillus amyloliquefaciens 0.291g, bacillus coagulans 0.188g, Lactobacillus plantarum 0.188g, lactobacillus acidophilus 0.188g, bafillus natto 0.47g, streptococcus fecalis 0.47g, lactobacillus 0.291g, saccharomycete 0.376g.

Embodiment 16

Select 9 kinds of microorganism fungus kinds for use, promptly produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, Lactobacillus plantarum, lactobacillus acidophilus, bafillus natto, streptococcus fecalis, lactobacillus, saccharomycete, preparation technology is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification combination → packing → finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02～0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.846g, bacillus amyloliquefaciens 0.47g, bacillus coagulans 0.291g, Lactobacillus plantarum 0.291g, lactobacillus acidophilus 0.291g, bafillus natto 0.658g, streptococcus fecalis 0.658g, lactobacillus 0.47g, saccharomycete 0.564g makes up and makes leavening will to measure the bacterium number.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹³Individual/g, content of ashes is less than 8%, and moisture is below 10%.

Embodiment 17

Select 9 kinds of microorganism fungus kinds for use, promptly produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, Lactobacillus plantarum, lactobacillus acidophilus, bafillus natto, streptococcus fecalis, lactobacillus, saccharomycete, preparation technology is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification combination → packing → finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02～0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.291g, bacillus amyloliquefaciens 0.094g, bacillus coagulans 0.094g, Lactobacillus plantarum 0.094g, lactobacillus acidophilus 0.094g, bafillus natto 0.291g, streptococcus fecalis 0.291g, lactobacillus 0.094g, saccharomycete 0.188g makes up and makes leavening will to measure the bacterium number.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹³Individual/g, content of ashes is less than 8%, and moisture is below 10%.

Embodiment 18

Select 9 kinds of microorganism fungus kinds for use, promptly produce yellowish fiber monad, bacillus amyloliquefaciens, bacillus coagulans, Lactobacillus plantarum, lactobacillus acidophilus, bafillus natto, streptococcus fecalis, lactobacillus, saccharomycete, preparation technology is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification combination → packing → finished product leavening.

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation is 20-48 hour under 25-35 ℃ of condition, puts into 4 ℃ of refrigerators and preserves.

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30 hour.

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02～0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33 hour.

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.564g, bacillus amyloliquefaciens 0.291g, bacillus coagulans 0.188g, Lactobacillus plantarum 0.188g, lactobacillus acidophilus 0.188g, bafillus natto 0.47g, streptococcus fecalis 0.47g, lactobacillus 0.291g, saccharomycete 0.376g makes up and makes leavening will to measure the bacterium number.

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹³Individual/g, content of ashes is less than 8%, and moisture is below 10%.

Embodiment 19

The preparation method of ruminant fermented feed is as follows:

The leavening activation

↓

The mixing of crushed stalk → dressing and stalk → pit entry fermentation (apparent temperature and decided 7-20 days) → finished product.

Dry straw is ground into 1-5 centimetre of section; With 0.1-0.5% syrup dissolving leavening, under 20-40 ℃ of state, to place and used later in 2-4 hour, the leavening after the activation used in 20 hours.

Dressing mixes with dry straw, and dry straw per ton adds dressing and the water 2t of sugared 1kg, corn 10kg, urea 10kg, salt 5kg, evenly admixes the leavening after the activation; The stalk that has added leavening and dressing is gone into the pond ferment, essential compacting and secluding air, fermentation time 7-20 day.

The finished product proterties: the color golden yellow has sour fragrance, the quality softness.

The fermented stalk feed proterties: digestibility improves 30-50% in the crude fibre degradation rate 10-17%, stalk body; Dry straw crude protein 3.2-4.5% before the fermentation, fermentation back crude protein content reaches 7.4-12%; Organic acid content reaches 2.0-1.6%; Moisture reaches 60-65%.

Various enzyme content: cellulase 100-112 unit/g, hemicellulase 15-20 unit/g, pectase 95-117 unit/g, proteinase 9 00-945 unit/g, glucan glycosides enzyme 80-87 unit/g.

Embodiment 20

The preparation method of ruminant fermented feed is as follows:

The leavening activation

↓

Crushed stalk → dressing and stalk mix → pack fermentation (apparent temperature and decided 7-20 days) → finished product.

Dry straw is ground into 1-5 centimetre of section; With 0.1-0.5% syrup dissolving leavening, under 20-40 ℃ of state, to place and used later in 2-4 hour, the leavening after the activation used in 20 hours.

Dressing mixes with dry straw, and dry straw per ton adds 1 ton in the dressing of sugared 0.1kg, corn 1kg, urea 1kg, salt 1kg and water, evenly admixes the leavening after the activation; The stalk pack of having added leavening and dressing is fermented essential compacting and secluding air, fermentation time 7-20 day.

The finished product proterties: the color golden yellow has sour fragrance, the quality softness.

The fermented stalk feed proterties: digestibility improves 30-50% in the crude fibre degradation rate 10-17%, stalk body; Dry straw crude protein 3.2-4.5% before the fermentation, fermentation back crude protein content reaches 7.4-12%; Organic acid content reaches 2.0-1.6%; Moisture reaches 60-65%.

Various enzyme content: cellulase 100-112 unit/g, hemicellulase 15-20 unit/g, pectase 95-117 unit/g, proteinase 9 00-945 unit/g, glucan glycosides enzyme 80-87 unit/g.

Embodiment 21

The preparation method of ruminant fermented feed is as follows:

The leavening activation

↓

Crushed stalk → dressing and stalk mix → pack into cylinder fermentation (apparent temperature and decided 7-20 days) → finished product.

Dry straw is ground into 1-5 centimetre of section; With 0.1-0.5% syrup dissolving leavening, under 20-40 ℃ of state, to place and used later in 2-4 hour, the leavening after the activation used in 20 hours.

Dressing mixes with dry straw, and dry straw per ton adds dressing and the water 1-2 ton of sugared 0.55kg, corn 5.5kg, urea 5.5kg, salt 3kg, evenly admixes the leavening after the activation; The stalk that has added leavening and dressing is gone into the pond ferment, essential compacting and secluding air, fermentation time 7-20 day.

The finished product proterties: the color golden yellow has sour fragrance, the quality softness.

The fermented stalk feed proterties: digestibility improves 30-50% in the crude fibre degradation rate 10-17%, stalk body; Dry straw crude protein 3.2-4.5% before the fermentation, fermentation back crude protein content reaches 7.4-12%; Organic acid content reaches 2.0-1.6%; Moisture reaches 60-65%.

Various enzyme content: cellulase 100-112 unit/g, hemicellulase 15-20 unit/g, pectase 95-117 unit/g, proteinase 9 00-945 unit/g, glucan glycosides enzyme 80-87 unit/g.

Embodiment 22

The preparation method of Nonruminantia or bird fermented feed is as follows:

The leavening activation

↓

The mixing of crushed stalk → dressing and stalk → pit entry fermentation (apparent temperature and decided 7-20 days) → finished product.

Dry straw is pulverized after 20 mesh sieves sieve; With 0.1-0.5% syrup dissolving leavening, under 20-40 ℃ of state, to place and used later in 2-4 hour, the leavening after the activation used up in 20 hours.

Dressing mixes with dry straw, and dry straw per ton adds dressing and the water 2t of sugared 1kg, corn 20kg, urea 10kg, salt 5kg, evenly admixes the leavening after the activation; The stalk that has added leavening and dressing is gone into the pond ferment, essential compacting and secluding air, fermentation time 7-20 day.

The finished product proterties: the color golden yellow has sour fragrance, the quality softness.

The fermented stalk feed proterties: digestibility improves 30-50% in the crude fibre degradation rate 10-17%, stalk body; Dry straw crude protein 3.2-4.5% before the fermentation, fermentation back crude protein content reaches 7.4-12%; Organic acid content reaches 2.0-1.6%; Moisture reaches 60-65%.

Various enzyme content: cellulase 100-112 unit/g, hemicellulase 15-20 unit/g, pectase 95-117 unit/g, proteinase 9 00-945 unit/g, glucan glycosides enzyme 80-87 unit/g.

Embodiment 23

The preparation method of Nonruminantia or bird fermented feed is as follows:

The leavening activation

↓

Crushed stalk → dressing and stalk mix → pack into bucket fermentation (apparent temperature and decided 7-20 days) → finished product.

Dry straw is pulverized after 40 mesh sieves sieve; With 0.1-0.5% syrup dissolving leavening, under 20-40 ℃ of state, to place and used later in 2-4 hour, the leavening after the activation used up in 20 hours.

Dressing mixes with dry straw, and dry straw per ton adds dressing and the water 1t of sugared 0.1kg, corn 5kg, urea 1kg, salt 1kg, evenly admixes the leavening after the activation; The stalk pack of having added leavening and dressing is fermented essential compacting and secluding air, fermentation time 7-20 day.

The finished product proterties: the color golden yellow has sour fragrance, the quality softness.

The fermented stalk feed proterties: digestibility improves 30-50% in the crude fibre degradation rate 10-17%, stalk body; Dry straw crude protein 3.2-4.5% before the fermentation, fermentation back crude protein content reaches 7.4-12%; Organic acid content reaches 2.0-1.6%; Moisture reaches 60-65%.

Various enzyme content: cellulase 100-112 unit/g, hemicellulase 15-20 unit/g, pectase 95-117 unit/g, proteinase 9 00-945 unit/g, glucan glycosides enzyme 80-87 unit/g.

Embodiment 24

The preparation method of Nonruminantia or bird fermented feed is as follows:

The leavening activation

↓

Crushed stalk → dressing and stalk mix → pack into cylinder fermentation (apparent temperature and decided 7-20 days) → finished product.

Dry straw is pulverized after 20 mesh sieves sieve; With 0.1-0.5% syrup dissolving leavening, under 20-40 ℃ of state, to place and used later in 2-4 hour, the leavening after the activation used up in 20 hours.

Dressing mixes with dry straw, and dry straw per ton adds dressing and the water 1.5t of sugared 0.55kg, corn 12.5kg, urea 5.5kg, salt 3kg, evenly admixes the leavening after the activation; The stalk that has added leavening and dressing is gone into the pond ferment, essential compacting and secluding air, fermentation time 7-20 day.

The finished product proterties: the color golden yellow has sour fragrance, the quality softness.

The fermented stalk feed proterties: digestibility improves 30-50% in the crude fibre degradation rate 10-17%, stalk body; Dry straw crude protein 3.2-4.5% before the fermentation, fermentation back crude protein content reaches 7.4-12%; Organic acid content reaches 2.0-1.6%; Moisture reaches 60-65%.

Various enzyme content: cellulase 100-112 unit/g, hemicellulase 15-20 unit/g, pectase 95-117 unit/g, proteinase 9 00-945 unit/g, glucan glycosides enzyme 80-87 unit/g.

Embodiment 25

Make the ruminant straw feed

The production technology of making ruminant straw feed leavening is:

Slant strains → liquid first order seed is cultivated (shaking a bottle cultivation) → liquid secondary seed cultivation (seeding tank) → fermentation tank expansion cultivation → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number and is measured → eight bacterial classifications are made up by proportioning and be made into leavening.

Every part 0.235 gram of eight bacterial classifications is respectively: produce yellowish fiber monad 0.17625g, bacillus amyloliquefaciens 0.235g, bacillus coagulans 0.235g, Lactobacillus plantarum 0.235g, propionibacterium freudenreichii 0.875g, streptococcus fecalis 0.875g, lactobacillus 0.235g, saccharomycete 0.47g, amount to 3 grams, 1 ton of dry straw ferments.

Use above-mentioned leavening and make the ruminant straw feed:

Agricultural crop straw is cut up with a hay cutter into 3-5cm length, add sugared 0.55kg, corn flour 5.5kg, urea 5.5kg, salt 5.5kg, water 1.5t, mix with stalk dry weight 1t, and the 3g leavening evenly added mixing, pit entry fermentation, essential compacting secluding air, fermentation time 7-40 day.Promptly obtain the ruminant straw fermented feed.

Embodiment 26

Make the non-ruminant animal straw fermented feed

The production technology of non-ruminant animal straw feed leavening is:

Slant strains → liquid first order seed is cultivated (shaking a bottle cultivation) → liquid secondary seed cultivation (seeding tank) → fermentation tank expansion cultivation → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number and is measured → eight bacterial classifications are made up by proportioning and be made into leavening.

Every part 0.194 gram of eight bacterial classifications is respectively: produce yellowish fiber monad 0.582g, bacillus amyloliquefaciens 0.485g, bacillus coagulans 0.194g, Lactobacillus plantarum 0.194g, lactobacillus acidophilus 0.194g, bafillus natto 0.485g, streptococcus fecalis 0.485g, candida utili 0.388g, amount to 3 grams, 1 ton of dry straw ferments.

Use above-mentioned leavening and make the non-ruminant animal straw feed:

Agricultural crop straw was pulverized 20 mesh sieves, with stalk dry weight 1t add sugared 0.55kg, corn flour 5.5kg, urea 5.5kg, salt 5.5kg, water 1.5t mixes, and the 3g leavening is evenly added mixing, pit entry fermentation, essential compacting secluding air, fermentation time 7-40 day.Promptly obtain the non-ruminant animal straw fermented feed.

Embodiment 27

Make the poultry straw fermented feed

The production technology of poultry animal straw feed leavening is:

Slant strains → liquid first order seed is cultivated (shaking a bottle cultivation) → liquid secondary seed cultivation (seeding tank) → fermentation tank expansion cultivation → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number and is measured → eight bacterial classifications are made up by proportioning and be made into leavening.

Every part 0.188 gram of eight bacterial classifications is respectively:

Produce yellowish fiber monad 0.564g, bacillus amyloliquefaciens 0.291g, bacillus coagulans 0.188g, Lactobacillus plantarum 0.188g, lactobacillus acidophilus 0.188g, bafillus natto 0.47g, streptococcus fecalis 0.47g, lactobacillus 0.291g, saccharomycete 0.376g, amount to 3 grams, 1 ton of dry straw ferments.

Use above-mentioned leavening and make the poultry straw feed:

Agricultural crop straw was pulverized 20 mesh sieves, added sugared 0.55kg, corn flour 5.5kg, urea 5.5kg, salt 5.5kg, water 1.5t, mixed with stalk dry weight 1t, and the 3g leavening evenly added mixing, pit entry fermentation, essential compacting secluding air, fermentation time 7-40 day.Promptly obtain the poultry straw fermented feed.

Claims (8)

1, one kind of multiple microbial straw fermented feeds and leavening and preparation method thereof is characterized in that ruminant fermented feed leavening comprises that following component forms by weight ratio:

Produce yellowish fiber monad 0.5-1 part, bacillus amyloliquefaciens 0.5-1.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, propionibacterium freudenreichii 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, lactobacillus 0.5-1.5 part, saccharomycete 1-3 part.

2, one kind of multiple microbial straw fermented feeds according to claim 1 and leavening and preparation method thereof is characterized in that selecting some kinds of microorganism fungus kinds for use by proportioning, and the technology of preparation ruminant fermented feed leavening is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification makes up in proportion → packs → the finished product leavening;

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation 20-48h under 25-35 ℃ of condition puts into 4 ℃ of refrigerators and preserves;

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h;

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33h;

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 0.5-1 part, bacillus amyloliquefaciens 0.5-1.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, propionibacterium freudenreichii 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, lactobacillus 0.5-1.5 part, saccharomycete 1-3 part is made up and is made leavening will to measure the bacterium number;

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹¹Individual/g, content of ashes is less than 8%, and moisture is below 10%.

3, one kind of multiple microbial straw fermented feeds and leavening and preparation method thereof is characterized in that non-ruminant animal fermented feed leavening comprises that following component forms by weight ratio:

Produce yellowish fiber monad 1.5-4.5 part, bacillus amyloliquefaciens 1.5-3.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, bafillus natto 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, candida utili 1-3 part.

4, one kind of multiple microbial straw fermented feeds according to claim 3 and leavening and preparation method thereof is characterized in that selecting some kinds of microorganism fungus kinds for use by proportioning, and the technology of preparation non-ruminant animal fermented feed leavening is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification makes up in proportion → packs → the finished product leavening;

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation 20-48h under 25-35 ℃ of condition puts into 4 ℃ of refrigerators and preserves;

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80～180 times/minute, inoculum concentration 1-3%, time 19-30 hour;

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02-0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33h;

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 1.5-4.5 part, bacillus amyloliquefaciens 1.5-3.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, bafillus natto 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, candida utili 1-3 part is made up and is made leavening will to measure the bacterium number;

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹¹Individual/g, content of ashes is less than 8%, and moisture is below 10%.

5, one kind of multiple microbial straw fermented feeds and leavening and preparation method thereof is characterized in that bird fermented feed leavening comprises that following component forms by weight ratio:

Produce yellowish fiber monad 1.5-4.5 part, bacillus amyloliquefaciens 0.5-2.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, bafillus natto 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, lactobacillus 0.5-2.5 part, saccharomycete 1-3 part.

6, one kind of multiple microbial straw fermented feeds according to claim 5 and leavening and preparation method thereof is characterized in that selecting some kinds of microorganism fungus kinds for use by proportioning, and the technology of preparation bird fermented feed leavening is as follows:

Slant strains cultivation → liquid first order seed (shaking bottle) cultivation → liquid secondary (seeding tank) cultivation → fermentation tank culture → supercentrifuge → vacuum freeze drier → pulverizer → bacterium number mensuration → bacterial classification makes up in proportion → packs → the finished product leavening;

Slant strains is cultivated: each bacterial classification uses corresponding culture medium and adds 1.5-2.5% agar, inoculum concentration 5-10%, and static cultivation 20-30h under 25-35 ℃ of condition puts into 4 ℃ of refrigerators and preserves;

Wherein shake a bottle condition of culture: temperature 25-40 ℃, rotating speed 80-180 time/minute, inoculum concentration 1-3%, time 19-30h;

Fermentation tank culture condition: temperature 25-40 ℃, ventilation 9-13m ³/ h, tank pressure 0.02～0.09Mpa, inoculum concentration 1.5-7.5%, time 19-33h;

Bacterial classification makes up in proportion: qualified product yellowish fiber monad 1.5-4.5 part, bacillus amyloliquefaciens 0.5-2.5 part, bacillus coagulans 0.5-1.5 part, Lactobacillus plantarum 0.5-1.5 part, lactobacillus acidophilus 0.5-1.5 part, bafillus natto 1.5-3.5 part, streptococcus fecalis 1.5-3.5 part, lactobacillus 0.5-2.5 part, saccharomycete 1-3 part is made up and is made leavening will to measure the bacterium number;

Leavening has following proterties:

Pale powder, tasteless or tart flavour slightly, viable count reaches 1.2 * 10 ¹³Individual/g, content of ashes is less than 8%, and moisture is below 10%.

7, realize the described multiple microbial straw fermented feed of claim 2 and leavening and preparation method thereof, it is characterized in that the preparation method of ruminant fermented feed is as follows:

The leavening activation

↓

Crushed stalk → dressing and stalk mix → pack into container or pit entry fermentation (apparent temperature and decided 7-30 days) → finished product;

Dry straw is ground into the 1-5cm section; With 0.1-0.5% syrup dissolving leavening, under 20-40 ℃ of state, to place and used later in 1-3 hour, the leavening after the activation used in 20 hours;

Dressing mixes with dry straw, and dry straw per ton adds dressing and the water 1-2t of sugared 0.1-1kg, corn 1-10kg, urea 1-5kg, salt 1-5kg, evenly admixes the leavening after the activation; With pack into container or go into the pond and ferment, essential compacting and secluding air, fermentation time 7-30 day of the stalk that has added leavening and dressing;

The finished product proterties: the color golden yellow has sour fragrance, the quality softness;

The fermented stalk feed proterties: digestibility improves 30-50% in the crude fibre degradation rate 10-17%, stalk body; Dry straw crude protein 3.2-4.5% before the fermentation, fermentation back crude protein content reaches 7.4-12%; Organic acid content reaches 2.0-1.6%; Moisture reaches 60-65%;

Various enzyme content: cellulase 100-112 unit/g, hemicellulase 15-20 unit/g, pectase 95-117 unit/g, proteinase 9 00-945 unit/g, glucan glycosides enzyme 80-87 unit/g.

8, realize claim 4,6 described multiple microbial straw fermented feeds and leavening and preparation method thereof, it is characterized in that the preparation method of Nonruminantia or bird fermented feed is as follows:

The leavening activation

↓

Crushed stalk → dressing and stalk mix → pack into container or pit entry fermentation (apparent temperature and decided 7-20 days) → finished product;

Dry straw is pulverized after 20 or 40 mesh sieves sieve; With 0.1-0.5% syrup dissolving leavening, under 20-40 ℃ of state, to place and used later in 2-4 hour, the leavening after the activation used up in 20 hours;

Dressing mixes with dry straw, and dry straw per ton adds dressing and the water 1-2t of sugared 0.1-1kg, corn 5-20kg, urea 1-10kg, salt 1-5kg, evenly admixes the leavening after the activation; With pack into container or go into the pond and ferment, essential compacting and secluding air, fermentation time 7-30 day of the stalk that has added leavening and dressing;

The finished product proterties: the color golden yellow has sour fragrance, the quality softness;

The fermented stalk feed proterties: digestibility improves 30-50% in the crude fibre degradation rate 10-17%, stalk body; Dry straw crude protein 3.2-4.5% before the fermentation, fermentation back crude protein content reaches 7.4-12%; Organic acid content reaches 2.0-1.6%; Moisture reaches 60-65%;

Various enzyme content: cellulase 100-112 unit/g, hemicellulase 15-20 unit/g, pectase 95-117 unit/g, proteinase 9 00-945 unit/g, glucan glycosides enzyme 80-87 unit/g.