CN1413477A - 骨乳多肽酸奶活菌制剂及制备方法 - Google Patents
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Abstract
本发明涉及一种含有小分子多肽及益生菌高密度活菌群的功能性微生态调节剂的配方及制备方法。其特征是利用骨蛋白液与鲜奶、低聚异麦芽糖配伍，经纳米级高频高压超微机处理后的营养成分更利于菌群繁殖、可做为高效培养基的特性，再经过浓缩、灭菌、冷却、复合益生菌发酵与木瓜蛋白酶酶解同步进行、冷却、真空冷冻干燥后，得到以骨乳酸奶为载体的、含有多肽化合物、活菌含量每克6000万以上的制剂。
Description
本发明涉及一种含有小分子多肽及益生菌高密度活菌群的功能性微生态调节剂的配方及其制备方法。产品是用嗜酸乳杆菌和嗜热链球菌发酵与木瓜蛋白酶解结合的方法，生产以骨乳酸奶为载体，以口服液、冻干粉、胶囊、片剂为剂型的促进健康食品。
在美国市场上销售的益生菌制剂与日本以双岐杆菌为主不同，主要是嗜酸乳杆菌制剂，商品大多冠以“ACI-DOPHILVS”，其中有的还含有双岐杆菌或其他的乳酸菌的多菌制剂。嗜酸乳杆菌也是人体正常菌群，它虽然栖息在人体小肠部，但它的整肠功能与双岐杆菌相同，其制造与保存比双岐杆菌容易，所以美国主要采用嗜酸乳杆菌。
酸奶是益生菌的传统来源，用于保健目的已有几百年历史了，各种果肉酸奶已不鲜见。经过黑龙江省科技厅信息中心2002年2月8日检索中国科技成果数据库、中国科技期刊数据库、中国专利数据库、中国学位论文数据库、中国学术会议数据库、生物文献数据库、维普行业剪报数据库、适用成果数据库后，出具的科技查新报告结论：本发明的骨乳多肽酸奶活菌制剂的配方、制备工艺及剂型未见报道。以奶和骨蛋白液为基料，将发酵与酶解相结合、高频高压条件下的纳米处理工艺、低温真空冷冻干燥工艺应用于酸奶制品生产中，得到含有小分子多肽及高密度益生菌群的产品，在各数据库中均未见报道。
本发明的目的是选择畜产品中有相辅相成作用的奶和骨蛋白液作为原辅材料，克服了传统酸奶活菌含量低、美容和调理胃肠功效短期内不明显、货架期短的弊端，提高功效、扩大用途，提高畜产品深加工附加值。为消费者提供易存储，运输方便、保质期长、兼具延缓衰老减少毒素产生、减轻口臭、抑制念珠菌生长、抑制肠道疾病、提高肌体免疫抗病能力等多种调理功能于一体的功能性健康食品---骨乳多肽酸奶活菌制剂。这是一种微生态调节剂。
本发明技术方案要点是：
1、在原料奶中添加骨蛋白液25％-40％，这种骨蛋白液为乳白色，比牛奶浓，比炼乳稀，固形物含量15％-30％，蛋白质含量9％-12％，与奶混合后，正好适宜木瓜蛋白酶水解对底物浓度的要求。添加这种骨蛋白液的目的是增加奶中蛋白及固形物含量，为菌群大量繁殖提供培养基的同时，充分发挥胶原蛋白美容养颜特殊作用。
2、将酶解与发酵有机的结合，应用于骨乳酸奶的制备工艺中，能使骨蛋白液中的胶原蛋白降解至肽及氨基酸。除利于吸收、提高美容养颜功效外，还为益生菌生长繁殖提供高效培养基，使活菌群超常增殖。通常医院或生产机构就用动物蛋白胨或牛肉膏做为细菌培养基。骨蛋白液性状类似蛋白胨，经过纳米化处理后，性能优于蛋白胨。
3、选择使用术瓜蛋白酶是由于这种酶除了对胶原蛋白有水解作用外，其水解条件偏酸性，PH值范围是5-7、温度45℃-50℃、时间7小时。与嗜热链球菌、嗜酸乳杆菌的发酵PH值范围、温度、时间接近，利于生产时操作。比如酸奶常规操作时发酵最佳PH值是5.4，发酵终点控制在PH4.5-4.7，培养温度控制在恒温43℃，培养时间一般3-4小时。考虑酶对发酵的影响，可以适当延长发酵时间。生产时将木瓜蛋白酶与菌种按酸奶生产规程操作，在物料温度45℃时，同时投入酶和菌，边水解边发酵。缩短生产周期，节约生产成本。
4、随着PH值下降，会对水解度产生抑制。为了解决部分底物水解不完全的问题，在杀菌前采用纳米化处理工艺，在纳米超微机工作压力达到150-200MPA，工作频率达到1兆赫以上高频高压条件下，蛋白质的分子大小平均化了，分子量降低，分散均匀，更利于水解及菌群繁殖，缩短了水解时间，使之与酸奶发酵同步完成。
5、将低温真空冷冻干燥工艺应用于骨乳多肽酸奶后期制备中，不但最大程度保持益生菌群及多肽的生物活性，而且改变了传统酸奶的产品形态，延长货架期，适应远距离运输。
实施例：选择新鲜牛奶为例
提示：全部生产过程应在GMP洁净10000级环境中完成。1、牛奶540--700kg、骨蛋白液250--400kg、低聚异麦芽糖20kg混匀搅拌。2、将搅匀的物料加热70℃，预热20分钟后，经150目筛网过滤。3、泵入能产生高频高压纳米级超微机中，处理后立即泵入浓缩罐。4、密度达到每毫升1.18---1.2时迅速杀菌，温度95℃-98℃加热10分钟。5、灭菌后经板式热交换器将温度迅速降至45---50℃。6、取酶活力为230万V/g的木瓜蛋白酶800g-1600g，用5L灭菌后的骨乳液稀释后，在搅拌下加入骨乳液中。7、将含嗜热链球菌10％-40％、嗜酸乳杆菌50％-85％的混合菌种经发酵剂、中间发酵剂、最后培养成生产发酵剂应用，按骨乳液的4％-10％加入搅拌10分钟。8、将温度控制在45℃，维持5小时。其间3---4小时之间，搅拌一次5分钟。9、随机检验菌群繁殖速度，活菌达到每克6000万以上时冷却至室温。(口服液灌装即可)10、冷却后的物料送入低温真空冷冻干燥机中冻干，得到冻干粉、装胶囊打片。
Claims (3)
1骨乳多肽酸奶活菌制剂及制备方法是在哺乳动物奶中添加从动物鲜骨中提取的骨蛋白液、低聚异麦芽糖，经高频高压纳米化处理、杀菌、冷却、酶解发酵、冷却、低温真空冷冻干燥方法完成，其特征是发酵与酶解同步进行，得到的制剂活菌含量达到每克6000万以上，而且含有小分子多肽。
2根据权利要求1骨乳多肽酸奶活菌制剂及制备方法，其特征是酶解所用的蛋白酶为木瓜蛋白酶。
3根据权利要求1骨乳多肽酸奶活菌制剂及制备方法，其特征是产品形式为口服液、冻干粉、胶囊、片剂。
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100415120C (zh) * | 2006-01-18 | 2008-09-03 | 内蒙古蒙牛乳业（集团）股份有限公司 | 一种益生菌制剂及其制备方法 |
| CN101496575B (zh) * | 2008-01-28 | 2010-10-20 | 中国食品发酵工业研究院 | 一种含短肽和益生菌的特殊营养食品配方 |
| CN101305751B (zh) * | 2008-06-19 | 2011-02-09 | 成都大学 | 一种多肽营养奶的加工方法 |
| CN102210454A (zh) * | 2011-04-29 | 2011-10-12 | 张玉国 | 一种微生物复合制剂及其制备方法 |
| CN101501059B (zh) * | 2006-05-31 | 2014-05-14 | Lfb生物科技公司 | 奶中一种或多种蛋白的提取方法 |
-
2002
- 2002-03-13 CN CN 02107603 patent/CN1209030C/zh not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100415120C (zh) * | 2006-01-18 | 2008-09-03 | 内蒙古蒙牛乳业（集团）股份有限公司 | 一种益生菌制剂及其制备方法 |
| CN101501059B (zh) * | 2006-05-31 | 2014-05-14 | Lfb生物科技公司 | 奶中一种或多种蛋白的提取方法 |
| CN101496575B (zh) * | 2008-01-28 | 2010-10-20 | 中国食品发酵工业研究院 | 一种含短肽和益生菌的特殊营养食品配方 |
| CN101305751B (zh) * | 2008-06-19 | 2011-02-09 | 成都大学 | 一种多肽营养奶的加工方法 |
| CN102210454A (zh) * | 2011-04-29 | 2011-10-12 | 张玉国 | 一种微生物复合制剂及其制备方法 |
| CN102210454B (zh) * | 2011-04-29 | 2012-12-05 | 张玉国 | 一种微生物复合制剂及其制备方法 |
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Effective date of registration: 20060630 Address after: 150300 A Cheng City Development Zone of Heilongjiang province Wuyi Road Longjianglong pharmaceutical Park Renlong company Patentee after: Heilongjiang Renlong Biotechnology Development Co Ltd Address before: 150001 Provincial Exhibition Hall, 40 west straight street, Heilongjiang, Harbin Patentee before: Zhao Weijie |
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Owner name: ZHAO WEIJIE Free format text: FORMER OWNER: HEILONGJIANG PROVINCE RENLONG BIOLOGY SCIENCE AND TECHNOLOGY DEVELOPMENT CO., LTD. Effective date: 20090703 |
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Effective date of registration: 20090703 Address after: Heilongjiang province Harbin city residential district A Cheng halal Longjianglong Building Room 401 Patentee after: Zhao Weijie Address before: The development zone of Heilongjiang province A Cheng Wuyi Road Longjianglong pharmaceutical Park Renlong company Patentee before: Heilongjiang Renlong Biotechnology Development Co Ltd |
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Address after: 301-1 room 10, building 90, Xiangjiang Road, Harbin District, Nangang Development Zone, Heilongjiang 150090, China Patentee after: Zhao Weijie Address before: 150300 Heilongjiang city of Harbin province A Cheng District Longjianglong halal District Building Room 401 Patentee before: Zhao Weijie |
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Addressee: Zhao Weijie Document name: Notification of Termination of Patent Right |
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Granted publication date: 20050706 Termination date: 20180313 |
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| CF01 | Termination of patent right due to non-payment of annual fee |