CN1350592B - 应用于原核生物和真核生物的基于核酸探针的诊断检验 - Google Patents

应用于原核生物和真核生物的基于核酸探针的诊断检验 Download PDF

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CN1350592B
CN1350592B CN00807503.4A CN00807503A CN1350592B CN 1350592 B CN1350592 B CN 1350592B CN 00807503 A CN00807503 A CN 00807503A CN 1350592 B CN1350592 B CN 1350592B
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托马斯·杰勒德·巴里
特伦斯·詹姆斯·史密斯
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Abstract

描述了能够作为核酸探针检验的靶点区来检测并鉴别原核生物和/或真核生物的ssrA基因或tmRNA即ssrA基因的RNA转录序列，或其片段。不同生物的tmRNA序列的核酸排列对比可用于鉴别序列内的同源区和非同源区，这些区域又可以用来设计属特异性和种特异性的寡聚核苷酸探针。这些新鉴定的同源和非同源区提供了在分子水平鉴别和检测生物体的基础。以此方式鉴别出的这种寡核苷酸探针可用于检测样品中的tmRNA，由此能够指示不同样品类型中存在的非病毒生物的生存能力。

Description

应用于原核生物和真核生物的基于核酸探针的诊断检验
技术领域
本发明涉及用于原核生物和/或真核生物的检测和鉴定的核酸检验中靶序列的鉴定。 
技术背景
ssrA基因编码小而稳定的高拷贝数的转录RNA序列(tmRNA)，该基因在所有的细菌中都存在，并且近来证实这种基因还存在于叶绿体和硅藻体中。tmRNA具有tRNA和mRNA分子的双重功能，并且参与补救失去终止密码子的截短的mRNA，促进截短的多肽在蛋白酶进行降解之前完成通读翻译(Keiler，K.C.et al.(1996)，Science，271，990-993)。tmRNA的这种特殊功能使得研究者开始对其分子的二级结构与功能之间的关系进行分析。这些研究集中在来自不同微生物的tmRNA的二级结构的保守性以及这种结构保守性在进化上的意义和功能相关性。Matveeva等(Matveeva.O et al(1998)，Vol.16，No.13，1374-1375)用tmRNA作为含有二级结构的RNA的模型研究了寡核苷酸与RNA分子的结合。研究没有实现鉴定tmRNA上的位点以及设计用于治疗目的的反义寡核苷酸的目标。 
现在用于细菌诊断的核酸靶点/探针的数量非常有限。因此，当前需要优先考虑鉴定出用于诊断目的的DNA和RNA靶点。用于发展探针的靶核苷酸序列例如可以是质粒，核糖体RNA基因，基因之间的序列，编码毒力因子的基因或随机基因组DNA片段。此外许多RNA分子已被人描述为可以作为基于RNA的检测的靶点如核糖体RNA和RNA酶P。 
任何基于核酸探针的检验都需要对当前所研究的真核生物和原核 生物的核酸序列的特征有清楚的了解。对原核生物或真核生物进行可靠的检测，所用核酸探针应该为高度特异性(即，和其它生物的核酸序列没有交叉反应)和高度敏感性(即，该生物的所有或多数株系都应该与探针反应并被检测到)。所以，优选的靶点序列应存在于目标生物体的所有的株系之中。该序列应具有显著的序列变异性以保证将目标物种与其它亲缘相近的物种能够区分开；但另一方面，应具有足够的序列保守性以保证能够检测出目标物种的所有株系。一般来说，对一个核酸序列的精确鉴定是进行核酸探针检验的基础，但精确鉴定是费时、困难和不确定的。到目前为止只有为数不多的几种方法能够用于发展核酸探针来进行较广范围的微生物检测。已经被证实可以作为开发探针中潜在可用的靶点的核酸序列包括如rRNA基因和RNA以及rRNA 16S/23S基因间序列。 
多数核酸探针/靶点检验都集中于用细菌的高拷贝核糖体RNA(rRNA)和rRNA 16S/23S间隔区序列(欧洲专利No.0395292)来进行检测和鉴定。市场上已经有的很多成功的商品化细菌检测试剂盒是基于rRNA探针/靶点来检测多种微生物。这包括由Genprobe Inc.(SanDiego California)出售的基于16S rRNA的一系列商品化探针诊断试剂盒，以及由Innogenetics N.V.(Ghent，Belgium)出售的基于16S/23S间隔区序列的DNA探针。但是大多数这种诊断试剂盒都有其局限性，包括由于靶点序列的低拷贝数造成的敏感性缺乏以及很多情况下由亲缘相近的生物体之间的序列一致性引起的特异性缺乏。 
能够直接应用于样品中指示其中存在的任何活的微生物的基于核酸的检测方法在食品、工业、环境和医学中的应用都有重大意义。 
基于DNA的检测方法的一个缺点是他们不能区分活的和死的生物体。一些研究致力于用rRNA和mRNA作为细胞存活的指示剂(Sheridan，G.E.C.et al.(1998)Applied and Environmental Microbiology，64，1313-1318)。但是这些序列并不是合适的靶点，因为rRNA和mRNA 在细菌死后在其细胞中还会存在48小时。 
随着基于核酸的类似微阵列形式的能同时对多核酸靶点进行监测的技术的出现，现在需要鉴别和表征新核酸序列作为探针和/或靶点区域来检测和鉴别活的真核细胞和原核细胞。 
发明公开 
本发明提供ssrA基因或其片段作为对原核生物或真核生物的核酸探针检验中的靶点区域。 
因此，本发明适用于除病毒外的所有生物体。 
尚没有有关使用ssrA基因的区域作为靶点区来检测和鉴别原核生物和真核生物的其它核酸探针检验及其相应的优势的报道。 
根据本发明的一个实施方案，ssrA基因分子中与从DNA分子5’末端起的高同源性区相对应的片段能够作为通用靶点区域。 
在本发明的另一个实施方案中，ssrA基因分子中与从DNA分子3’末端起的高同源性区相对应的片段能够作为通用靶点区域。 
在本发明的一个进一步的实施方案中，ssrA基因分子中与低同源性区域相对应的片段能够作为区分不同物种的核酸探针检验的靶点区。 
在本发明的另一个进一步的实施方案中，ssrA基因分子中与低同源性区域相对应的片段能够作为用来产生物属特异性探针的靶点区。 
如下文所描述，不同生物体的ssrA基因的核酸序列的对比表明这些分子的5’和3’区具有高度同源性，所以可以用为通用靶点序列区。 在亲缘相近的生物体间，ssrA基因比其它细菌高拷贝数RNA有更显著程度的序列变异性。这些变异区可作为区分不同物种的核酸检验的理想靶点。 
本发明还提供了tmRNA即ssrA基因的RNA转录序列或其片段用作为对原核生物或真核生物的核酸探针检验中的靶点区域。 
根据本发明这一方面的一个实施方案，tmRNA分子中与从tmRNA5’末端起的一段高同源性区相对应的片段能够作为通用靶点区。 
可替代地，tmRNA中与从tmRNA 3’末端起的一段高同源性区相对应的片段能够作为通用靶点区。 
根据本发明这一方面的一个进一步实施方案，tmRNA分子中与低同源性区相对应的片段能够作为区分不同物种的核酸探针检验的靶点区。 
根据本发明的另一个进一步的实施方案，tmRNA分子中与低同源性区域相对应的片段能够作为用来产生属特异性探针的靶点区。 
根据本发明的核酸探针(DNA或RNA)典型地含有来自于ssrA基因或tmRNA转录序列或它们的互补序列的至少10个核苷酸，这些探针被用于真核生物或原核生物的核酸探针杂交检验。探针与它的互补序列的杂交典型地通过在核酸探针上标记上放射性或非放射性(如比色的或荧光的)标记物来揭示。 
在优选的实施方案中，所述的ssrA基因片段或tmRNA片段可作为用于扩增过程中所用的引物的基础。 
与ssrA基因或tmRNA序列的5’或3’区相对应的通用寡核苷酸引 物可按照本发明使用，以便从广泛的细菌谱中扩增ssrA基因或它所编码的tmRNA，有助于实现大范围生物体的同时扩增，同时能使特异性的核酸探针发生杂交并进行检测。 
优选地，扩增过程的产物可以作为核酸探针检验中的靶点区。 
更加优选地，tmRNA分子的cDNA转录序列被作为探针用于核酸杂交检验。 
这种检测可以在体外完成或在原位完成。 
这里所定义的靶点区能够作为区分活的和死的原核生物或真核生物的检测的基础。
与细菌死后存在于其细胞中的rRNA和mRNA不同，tmRNA在死亡的生物体中迅速降解。因此，tmRNA能够作为有用的靶点来区分死的和活的原核生物或真核生物，即，通过核酸探针直接与分离的细菌RNA杂交或者通过将RNA扩增并将扩增产物和核酸探针杂交来将死的和活的原核生物或真核生物进行区分。 
优选地，靶点区以多种探针形式使用，以大范围地检测和/或鉴定原核生物或真核生物。 
根据本发明的ssrA基因探针或tmRNA转录序列探针可以被连接到微阵列基因芯片***上，以大范围地对原核生物或真核生物进行高通量的检测和鉴定。 
根据本发明的靶点区能够作为试验中的探针来检测目标样品中的原核生物或真核生物。 
上述目标样品包括生物样品例如呼吸道的组织样品，泌尿生殖道的组织样品或胃肠道的组织样品，或者体液如血液和血液组分，唾液或脑脊液。 
根据本发明的检验还可以在其它样品中完成，如食品样品，环境样品包括空气、水、海洋和土壤样品，以及植物和动物来源的样品。 
根据本发明，ssrA基因或tmRNA转录序列的片段可被用于获得原核生物或真核生物的DNA图谱的检验中，并因此能够区分同一物种的不同株系。 
核酸序列的对比表明在每一物种之内都会在ssrA基因和tmRNA转录序列内发生序列变异。种内序列变异性能被用来区分同一物种的不同株系来研究流行病学、追踪例如爆发时的感染媒介，或者进行种群研究。 
本发明的其它应用包括用ssrA基因，tmRNA转录序列或与其互补的DNA序列或其片段来设计用于治疗目的的试剂来对抗感染性的原核生物或真核生物。 
这些试剂可包括反义mRNA或寡核苷酸、核酶和拮抗性多肽并且可以任何药学上适用的形式来使用。 
因此，本发明通过使用tmRNA靶点能被用于只检测生物样品中存活的生物体。因此，在任何抗感染药物治疗期间和之后，tmRNA靶点都能够用于监测对于这些特异性感染媒介(如抗微生物和/或抗寄生虫治疗)的治疗效率。 
在一个实施方案中，靶点区被用来监测药物对感染媒介的治疗效率。 
在另一个实施方案中，靶点区被用来监测胃肠道中促进健康的微生物的存活率和水平。 
本发明的这一方面涉及，例如，将包含于如酸乳酪或其它促进健康的食品中的促进健康(益生的)的微生物菌群引入肠道。能够持续监测这些微生物的存在及水平对于保证它们持续促进健康的功能是有必要的。tmRNA区能够作为检测存活的微生物，例如在粪便中的微生物的靶点，以监测促进健康的生物体的存在。 
在一个进一步的实施方案中，检验被用于对原核生物或真核生物进行定量分析。 
当使用探针杂交和/或体外扩增来检测样品中的微生物时，可以通过信号强度来测定所存在的微生物的数量。实时体外扩增的方法能被用来对样品中的生物体进行定量分析。因此，对样品中的生物体进行定量分析的能力对用于治疗目的的临床情况是非常重要的，如监测抗生素治疗或其它治疗手段或治疗效率。 
本发明的另一个进一步的应用是使用ssrA基因序列的数据库来鉴定原核生物或真核生物。 
本发明提供一系列用于ssrA基因和tmRNA序列5’和3’端的同源区和变异区的探针，这些探针来自于这些序列或这些序列的互补序列。下面为代表性的序列： 
伴放线菌素放线杆菌(Actinobacillus actinomycetemcomitans)ssrA 
GGGGCTGATTCTGGATTCGACGGGATTAGCGAAGCCCGAAGTGCACGTCGAGGTGCGGTAGGCCTCGTAAATAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCC TTTAGCCTTCGCTCCCCAGCTTCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGATCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAAGTAGCTTAATTGTCGCGTGTCCGTCAGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA SEQ ID NO：1 
伴放线菌素放线杆菌(Actinobacillus actinomycetemcomitans)tmRNA 
GGGGCUGAUUCUGGAUUCGACGGGAUUAGCGAAGCCCGAAGUGCACGUCGAGGUGCGGUAGGCCUCGUAAAUAAACCGCAAAAAAAUAGUCGCAAACGACGAACAAUACGCUUUAGCAGCUUAAUAACCUGCCUUUAGCCUUCGCUCCCCAGCUUCCGCUCGUAAGACGGGGAUAAAGCGGAGUCAAACCAAAACGAGAUCGUGUGGAAGCCACCGUUUGAGGAUCGAAGCAUUAAAUUAAAUCAAAGUAGCUUAAUUGUCGCGUGUCCGUCAGCAGGAUUAAGUGAAUUUAAAGACCGGACUAAACGUGUAGUGCUAACGGCAGAGGAAUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA SEQ ID NO：2 
杀鲑气单胞菌(Aeromonas salmonicida)ssrA，内部部分 
AAGATTCACGAAACCCAAGGTGCATGCCGAGGTGCGGTAGGCCTCGTTAACAAACCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCACTAGCAGCtTAATAACCTGCATAGAGCCCTTCTACCCTAGCTT  GCCTGTGTCCTAGGGAATCGGAAGGTCATCCTTCACAGGATCGTGTGGAAGTCCTGCTCGGGGCGGAAGCATTAAAACCAATCGAGCTAGTCAATTCGTGGCGTGTCTCTCCGCAGCGGGTTGGCGAATGTAAAGAGTGACTAAGCATGTAGTACCGAGGATGTAGTAATTTTGGACGGGG SEQ ID NO：3 
杀鲑气单胞菌(Aeromonas salmonicida)tmRNA，内部部分 
AAGAUUCACGAAACCCAAGGUGCAUGCCGAGGUGCGGUAGGCC UCGUUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCACUAGCAGCUUAAUAACCUGCAUAGAGCCCUUCUACCCUAGCUUGCCUGUGUCCUAGGGAAUCGGAAGGUCAUCCUUCACAGGAUCGUGUGGAAGUCCUGCUCGGGGCGGAAGCAUUAAAACCAAUCGAGCUAGUCAAUUCGUGGCGUGUCUCUCCGCAGCGGGUUGGCGAAUGUAAAGAGUGACUAAGCAUGUAGUACCGAGGAUGUAGUAAUUUUGGACGGGG SEQ ID NO：4 
富养产碱菌(Alcaligenes eutrophus)ssrA 
TGGGCCGACCTGGTTTCGACGTGGTTACAAAGCAGTGAGGCATACCGAGGACCCGTCACCTCGTTAATCAATGGAATGCAATAACTGCTAACGACGAACGTTACGCACTCGCTTAATTGCGGCCGTCCTCGCACTGGCTCGCTGACGGGCTAGGGTCGCAAGACCACGCGAGGTATTTACGTCAGATAAGCTCCGGAAGGGTCACGAAGCCGGGGACGAAAACCTAGTGACTCGCCGTCGTAGAGCGTGTTCGTCCGATGCGCCGGTTAAATCAAATGACAGAACTAAGTATGTAGAACTCTCTGTGGAGGGCTTACGGACGCGGGTTCGATTCCCGCCGGCTCCACCA SEQ IDNO：5 
富养产碱菌(Alcaligenes eutrophus)tmRNA 
UGGGCCGACCUGGUUUCGACGUGGUUACAAAGCAGUGAGGCAUACCGAGGACCCGUCACCUCGUUAAUCAAUGGAAUGCAAUAACUGCUAACGACGAACGUUACGCACUCGCUUAAUUGCGGCCGUCCUCGCACUGGCUCGCUGACGGGCUAGGGUCGCAAGACCACGCGAGGUAUUUACGUCAGAUAAGCUCCGGAAGGGUCACGAAGCCGGGGACGAAAACCUAGUGACUCGCCGUCGUAGAGCGUGUUCGUCCGAUGCGCCGGUUAAAUCAAAUGACAGAACUAAGUAUGUAGAACUCUCUGUGGAGGGCUUACGGACGCGGGUUCGAUUCCCGCCGGCUCCACCA SEQ ID NO：6 
产液菌(Aquifex aeolicus)ssrA 
GGGGGCGGAAAGGATTCGACGGGGACAGGCGGTCCCCGAGGAGCAGGCCGGGTGGCTCCCGTAACAGCCGCTAAAACAGCTCCCGAAGCTGAACTCGCTCTCGCTGCCTAATTAAACGGCAGCGCGTCCCCGGTAGGTTTGCGGGTGGCCTACCGGAGGGCGTCAGAGACACCCGCTCGGGCTACTCGGTCGCACGGGGCTGAGTAGCTGACACCTAACCCGTGCTACCCTCGGGGAGCTTGCCCGTGGGCGACCCGAGGGGAAATCCTGAACACGGGCTAAGCCTGTAGAGCCTCGGATGTGGCCGCCGTCCTCGGACGCGGGTTCGATTCCCGCCGCCTCCACCASEQ ID NO：7 
产液菌(Aquifex aeolicus)tmRNA 
GGGGGCGGAAAGGAUUCGACGGGGACAGGCGGUCCCCGAGGAGCAGGCCGGGUGGCUCCCGUAACAGCCGCUAAAACAGCUCCCGAAGCUGAACUCGCUCUCGCUGCCUAAUUAAACGGCAGCGCGUCCCCGGUAGGUUUGCGGGUGGCCUACCGGAGGGCGUCAGAGACACCCGCUCGGGCUACUCGGUCGCACGGGGCUGAGUAGCUGACACCUAACCCGUGCUACCCUCGGGGAGCUUGCCCGUGGGCGACCCGAGGGGAAAUCCUGAACACGGGCUAAGCCUGUAGAGCCUCGGAUGUGGCCGCCGUCCUCGGACGCGGGUUCGAUUCCCGCCGCCUCCACCA SEQ ID NO：8 
巨大芽孢杆菌(Bacillus megaterium)ssrA，内部部分 
AGGGTAGTTCGAGCTTAGGTTGCGAGTCGAGGAGATGGCCTCGTTAAAACATCAACGCCAATAATAACTGGCAAATCTAACAATAACTTCGCTTTAGCTGCATAATAGTAGCTTAGCGTTCCTCCCTCCATCGCCCATGTGGTAGGGTAAGGGACTCACTTTAAGTGGGCTACGCCGGAGTTCGCCGTCTGAGGACGAAGGAAGAGAATAATCAGACTAGCGACTGGGACGCCTGTTGGTAGGCAGAACAGCTCGCGAATGATCAATATGCCAACTACACTCGTAGACGCTTAAGTGGCCATATTTCTGGAC GTGG SEQ ID NO：9 
巨大芽孢杆菌(Bacillus megaterium)tmRNA，内部部分 
AGGGUAGUUCGAGCUUAGGUUGCGAGUCGAGGAGAUGGCCUCGUUAAAACAUCAACGCCAAUAAUAACUGGCAAAUCUAACAAUAACUUCGCUUUAGCUGCAUAAUAGUAGCUUAGCGUUCCUCCCUCCAUCGCCCAUGUGGUAGGGUAAGGGACUCACUUUAAGUGGGCUACGCCGGAGUUCGCCGUCUGAGGACGAAGGAAGAGAAUAAUCAGACUAGCGACUGGGACGCCUGUUGGUAGGCAGAACAGCUCGCGAAUGAUCAAUAUGCCAACUACACUCGUAGACGCUUAAGUGGCCAUAUUUCUGGACGUGG SEQ ID NO：10
枯草芽孢杆菌(Bacillus subtilis)ssrA 
GGGGACGTTACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAGCTGCCTAATAAGCGCAGCGAGCTCTTCCTGACATTGCCTATGTGTCTGTGAAGAGCACATCCAAGTAGGCTACGCTTGCGTTCCCGTCTGAGAACGTAAGAAGAGATGAACAGACTAGCTCTCGGAAGGCCCGCCCGCAGGCAAGAAGATGAGTGAAACCATAAATATGCAGGCTACGCTCGTAGACGCTTAAGTAATCGATGTTTCTGGACGTGGGTTCGACTCCCACCGTCTCCACCA SEQ ID NO：11
枯草芽孢杆菌(Bacillus subtilis)tmRNA 
GGGGACGUUACGGAUUCGACAGGGAUGGAUCGAGCUUGAGCUGCGAGCCGAGAGGCGAUCUCGUAAACACGCACUUAAAUAUAACUGGCAAAACUAACAGUUUUAACCAAAACGUAGCAUUAGCUGCCUAAUAAGCGCAGCGAGCUCUUCCUGACAUUGCCUAUGUGUCUGUGAAGAGCACAUCCAAGUAGGCUACGCUUGCGUUCCCGUCUGAGAACGUAAGAAGAGAUGAACAGACUAGCUCUCGGAAGGCCCGCC CGCAGGCAAGAAGAUGAGUGAAACCAUAAAUAUGCAGGCUACGCUCGUAGACGCUUAAGUAAUCGAUGUUUCUGGACGUGGGUUCGACUCCCACCGUCUCCACCA SEQ ID NO：12
百日咳鲍特氏菌(Bordetella pertussis)ssrA 
GGGGCCGATCCGGATTCGACGTGGGTCATGAAACAGCTCAGGGCATGCCGAGCACCAGTAAGCTCGTTAATCCACTGGAACACTACAAACGCCAACGACGAGCGTCTCGCTCTCGCCGCTTAAGCGGTGAGCCGCTGCACTGATCTGTCCTTGGGTCAGGCGGGGGAAGGCAACTTCACAGGGGGCAACCCCGAACCGCAGCAGCGACATTCACAAGGAATCGGCCACCGCTGGGGTCACACGGCGTTGGTTTAAATTACGTGAATCGCCCTGGTCCGGCCCGTCGATCGGCTAAGTCCAGGGTTAAATCCAAATAGATCGACTAAGCATGTAGAACTGGTTGCGGAGGGCTTGCGGACGGGGGTTCAATTCCCCCCGGCTCCACCASEQ ID NO：13
百日咳鲍特氏菌(Bordetella pertussis)tmRNA 
GGGGCCGAUCCGGAUUCGACGUGGGUCAUGAAACAGCUCAGGGCAUGCCGAGCACCAGUAAGCUCGUUAAUCCACUGGAACACUACAAACGCCAACGACGAGCGUCUCGCUCUCGCCGCUUAAGCGGUGAGCCGCUGCACUGAUCUGUCCUUGGGUCAGGCGGGGGAAGGCAACUUCACAGGGGGCAACCCCGAACCGCAGCAGCGACAUUCACAAGGAAUCGGCCACCGCUGGGGUCACACGGCGUUGGUUUAAAUUACGUGAAUCGCCCUGGUCCGGCCCGUCGAUCGGCUAAGUCCAGGGUUAAAUCCAAAUAGAUCGACUAAGCAUGUAGAACUGGUUGCGGAGGGCUUGCGGACGGGGGUUCAAUUCCCCCCGGCUCCACCASEQ ID NO：14
布氏疏螺旋体(Borrelia burgdorferi)ssrA 
GGGGATGTTTTGGATTTGACTGAAAATGTTAATATTGTAAGTTGC AGGCAGAGGGAATCTCTTAAAACTTCTAAAATAAATGCAAAAAATAATAACTTTACAAGCTCAAATCTTGTAATGGCTGCTTAAGTTAGCAGAGGGTTTTGTTGAATTTGGCTTTGAGGTTCACTTATACTCTTTTCGACATCAAAGCTTGCTTAAAAATGTTTTCAAGTTGATTTTTAGGGACTTTTATACTTGAGAGCAATTTGGTGGTTTGCTAGTATTTCCAAACCATATTGCTTAATAAAATACTAGATAAGCTTGTAGAAGCTTATAGTATTATTTTTAGGACGCGGGTTCAATTCCCGCCATCTCCACCA SEQ ID NO：15
布氏疏螺旋体(Borrelia burgdorferi)tmRNA 
GGGGAUGUUUUGGAUUUGACUGAAAAUGUUAAUAUUGUAAGUUGCAGGCAGAGGGAAUCUCUUAAAACUUCUAAAAUAAAUGCAAAAAAUAAUAACUUUACAAGCUCAAAUCUUGUAAUGGCUGCUUAAGUUAGCAGAGGGUUUUGUUGAAUUUGGCUUUGAGGUUCACUUAUACUCUUUUCGACAUCAAAGCUUGCUUAAAAAUGUUUUCAAGUUGAUUUUUAGGGACUUUUAUACUUGAGAGCAAUUUGGUGGUUUGCUAGUAUUUCCAAACCAUAUUGCUUAAUAAAAUACUAGAUAAGCUUGUAGAAGCUUAUAGUAUUAUUUUUAGGACGCGGGUUCAAUUCCCGCCAUCUCCACCA SEQ ID NO：16
空肠弯曲杆菌(Campylobacter jejuni)ssrA 
GGGAGCGACTTGGCTTCGACAGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGGCAAAGCGTAAAAAGCCCAAATAAAATTAAACGCAAACAACGTTAAATTCGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCTGAAATTTAAGTCATACTATCTAGCTTAATTTTCGGTCATTTTTGATAGTGTAGCCTTGCGTTTGACAAGCGTTGAGGTGAAATAAAGTCTTAGCCTTGCTTTTGAGTTTTGGAAGATGAGCGAAGTAGGGTGAAGTAGTCATCTTTGCTAAGCATGTAGAGGTCTTTGTGGGATTATTTTTGGACAGGGGTTCGATTCCCCTCGCTTCCACCASEQ ID NO：17 
空肠弯曲杆菌(Campylobacter jejuni)tmRNA 
GGGAGCGACUUGGCUUCGACAGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGGCAAAGCGUAAAAAGCCCAAAUAAAAUUAAACGCAAACAACGUUAAAUUCGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCUGAAAUUUAAGUCAUACUAUCUAGCUUAAUUUUCGGUCAUUUUUGAUAGUGUAGCCUUGCGUUUGACAAGCGUUGAGGUGAAAUAAAGUCUUAGCCUUGCUUUUGAGUUUUGGAAGAUGAGCGAAGUAGGGUGAAGUAGUCAUCUUUGCUAAGCAUGUAGAGGUCUUUGUGGGAUUAUUUUUGGACAGGGGUUCGAUUCCCCUCGCUUCCACCA SEQ ID NO：18
沙眼衣原体(Chlamydia trachomatis(D/UW-3/CX))ssrA 
GGGGGTGTAAAGGTTTCGACTTAGAAATGAAGCGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCGACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATCAGCTTCGCTGATCTCGAAGATCTAAGAGTAGCTGCTTAATTAGCAAAGTTGTTACCTAAATACGGGTGACCCGGTGTTCGCGAGCTCCACCAGAGGTTTTCGAAACACCGTCATGTATCTGGTTAGAACTTAGGTCCTTTAATTCTCGAGGAAATGAGTTTGAAATTTAATGAGAGTCGTTAGTCTCTATAGGGGTTTCTAGCTGAGGAGACATAACGTATAGTACCTAGGAACTAAGCATGTAGAGGTTAGCGGGGAGTTTACTAAGGACGAGAGTTCGACTCTCTCCACCTCCACCA SEQ ID NO：19
沙眼衣原体(Chlamydia trachomatis(D/UW-3/CX))tmRNA 
GGGGGUGUAAAGGUUUCGACUUAGAAAUGAAGCGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCGACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUCAGCUUCGCUGAUCUCGAAGAUCUAAGAGUAGCUGCUUAAUUAGCAAAGUUGUUACCUAAAUACGGGUGACCCGGUGUUCGCGAGCUCCACCAGA GGUUUUCGAAACACCGUCAUGUAUCUGGUUAGAACUUAGGUCCUUUAAUUCUCGAGGAAAUGAGUUUGAAAUUUAAUGAGAGUCGUUAGUCUCUAUAGGGGUUUCUAGCUGAGGAGACAUAACGUAUAGUACCUAGGAACUAAGCAUGUAGAGGUUAGCGGGGAGUUUACUAAGGACGAGAGUUCGACUCUCUCCACCUCCACCASEQ ID NO：20
沙眼衣原体(鼠肺炎)(Chlamydia trachomatis(mouse pneumonitis))ssrA 
GGGGGTGTAAAGGTTTCGACTTAGAAATGAAGCGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCGACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATCAGCTTCGCTGATCTTAATGATCTAAGAGTTGCTGCTTAATTAGCAAAGTTGTTACCTAAGTACTGGTAACCCGGTGTTCGCGAGCTCCACCAGAGGTTTTCGAAACGCCGTCATTTATCTGGTTAGAATTAGGGCCTTTTAACTCTCAAGGGAACTAATTTGAATTTTAATGAGAGTCGTTGGTCTCTATAGAGGTTTCTAGCTGAGGAGATATAACGTAAAATATTCTAGAAACTAAGCATGTAGAGGTTAGCGGGGAGTTTACTAAGGACGAGAGTTCGAATCTCTCCACCTCCACCA SEQ ID NO：21
沙眼衣原体(鼠肺炎)(Chlamydia trachomatis(mouse pneumonitis))tmRNA 
GGGGGUGUAAAGGUUUCGACUUAGAAAUGAAGCGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCGACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUCAGCUUCGCUGAUCUUAAUGAUCUAAGAGUUGCUGCUUAAUUAGCAAAGUUGUUACCUAAGUACUGGUAACCCGGUGUUCGCGAGCUCCACCAGAGGUUUUCGAAACGCCGUCAUUUAUCUGGUUAGAAUUAGGGCCUUUUAACUCUCAAGGGAACUAAUUUGAAUUUUAAUGAGAGUCGUUGGUCUCUAUAGAGGUUUCUAGCUGAGGAGAUAUAACGUAAAAUAUUCUAGAAACUAAGCAUGUAGAGGUUAGCGGGGAGUUUACU AAGGACGAGAGUUCGAAUCUCUCCACCUCCACCASEQ ID NO：22
微温杆状绿菌(Chlorobium tepidum)ssrA 
GGGGATGACAGGCTATCGACAGGATAGGTGTGAGATGTCGTTGCACTCCGAGTTTCAGCATGGACGGACTCGTTAAACAAGTCTATGTACCAATAGATGCAGACGATTATTCGTATGCAATGGCTGCCTGATTAGCACAAGTTAATTCAGAAGCCATCGTCCTGCGGTGAATGCGCTTACTCTGAAGCCGCCGGATGGCATAACCCGCGCTTGAGCCTACGGGTTCGCGCAAGTAAGCTCCGTACATTCATGCCCGAGGGGGTGTGCGGGTAACCAATCGGGATAAGGGGACGAACGCTGCTGGCGGTGTAATCGGACCACGAAAAACCAACCACCAGAGATGAGTGTGGTAACTGCATCGAGCAGTGTCCTGGACGCGGGTTCAAGTCCCGCCATCTCCACCA SEQ ID NO：23
微温杆状绿菌(Chlorobium tepidum)tmRNA 
GGGGAUGACAGGCUAUCGACAGGAUAGGUGUGAGAUGUCGUUGCACUCCGAGUUUCAGCAUGGACGGACUCGUUAAACAAGUCUAUGUACCAAUAGAUGCAGACGAUUAUUCGUAUGCAAUGGCUGCCUGAUUAGCACAAGUUAAUUCAGAAGCCAUCGUCCUGCGGUGAAUGCGCUUACUCUGAAGCCGCCGGAUGGCAUAACCCGCGCUUGAGCCUACGGGUUCGCGCAAGUAAGCUCCGUACAUUCAUGCCCGAGGGGGUGUGCGGGUAACCAAUCGGGAUAAGGGGACGAACGCUGCUGGCGGUGUAAUCGGACCACGAAAAACCAACCACCAGAGAUGAGUGUGGUAACUGCAUCGAGCAGUGUCCUGGACGCGGGUUCAAGUCCCGCCAUCUCCACCA SEQ ID NO：24
Cyanophora paradoxa(藻类)蓝色小体ssrA 
GGGGCTGTTTAGGTTTCGACGTTTTTTTCTAATTATGTTTGTTAAGCAAGTCGAGGATTTGTTCTATCTCGAAAATCAAGAACTCTCAAAA TTTAAACGCAACTAATATTGTACGTTTTAACCGTAAAGCAGCTTTCGCTGTTTAATAATTACTTTTAATTTAAAAACCTAATTTTTTTAGGAATTTATTTATTTATTGTTTATCCTGCTTAATGAATTAAAAAAAGCTATACTTGTGAATAAACGCATAATTTAAAAAAACGGACGTGGGTTCAAATCCCACCAGCTCCACCA SEQ ID NO：25
Cyanophora paradoxa(藻类)蓝色小体tmRNA 
GGGGCUGUUUAGGUUUCGACGUUUUUUUCUAAUUAUGUUUGUUAAGCAAGUCGAGGAUUUGUUCUAUCUCGAAAAUCAAGAACUCUCAAAAUUUAAACGCAACUAAUAUUGUACGUUUUAACCGUAAAGCAGCUUUCGCUGUUUAAUAAUUACUUUUAAUUUAAAAACCUAAUUUUUUUAGGAAUUUAUUUAUUUAUUGUUUAUCCUGCUUAAUGAAUUAAAAAAAGCUAUACUUGUGAAUAAACGCAUAAUUUAAAAAAACGGACGUGGGUUCAAAUCCCACCAGCUCCACCASEQ ID NO：26
丙酮丁醇梭菌(Clostridium acetobutylicum)ssrA，3′部分 
AATCTGGCGTCGAGAGCGGGGAAACGAGCCTTACAAAGCTTTGAGTAAGGAACGGAATTTATGAAGCTACTGAAGTGAAAAGCTTGTTTGTAGGCGTTTCATGGAGGGAATGTTAAAATACAAACTGCACTCGGAGATGCTTAATGAAACCATTTTCGGACAGGGGTTCGATTCCCCTCGCCTCCACCA SEQ ID NO：27
丙酮丁醇梭菌(Clostridium acetobutylicum)tmRNA，3′部分 
AAUCUGGCGUCGAGAGCGGGGAAACGAGCCUUACAAAGCUUUGAGUAAGGAACGGAAUUUAUGAAGCUACUGAAGUGAAAAGCUUGUUUGUAGGCGUUUCAUGGAGGGAAUGUUAAAAUACAAACUGCACUCGGAGAUGCUUAAUGAAACCAUUUUCGGACAGGGGUUCGAUUCCCCUCGCCUCCACCA SEQ ID NO：28 
放射异常球菌模式种(Deinococcus radiodurans)ssrA 
GGGGGTGACCCGGTTTCGACAGGGGAACTGAAGGTGATGTTGCGTGTCGAGGTGCCGTTGGCCTCGTAAACAAACGGCAAAGCCATTTAACTGGCAACCAGAACTACGCTCTCGCTGCTTAAGTGAGATGACGACCGTGCAGCCCGGCCTTTGGCGTCGCGGAAGTCACTAAAAAAGAAGGCTAGCCCAGGCGATTCTCCATAGCCGACGGCGAAACTTTATGGAGCTACGGCCTGCGAGAACCTGCCCACTGGTGAGCGCCGGCCCGACAATCAAACAGTGGGATACACACGTAGACGCACGCTGGACGGACCTTTGGACGGCGGTTCGACTCCGCCCACCTCCACCASEQ ID NO：29
放射异常球菌模式种(Deinococcus radiodurans)tmRNAGGGGGUGACCCGGUUUCGACAGGGGAACUGAAGGUGAUGUUGCGUGUCGAGGUGCCGUUGGCCUCGUAAACAAACGGCAAAGCCAUUUAACUGGCAACCAGAACUACGCUCUCGCUGCUUAAGUGAGAUGACGACCGUGCAGCCCGGCCUUUGGCGUCGCGGAAGUCACUAAAAAAGAAGGCUAGCCCAGGCGAUUCUCCAUAGCCGACGGCGAAACUUUAUGGAGCUACGGCCUGCGAGAACCUGCCCACUGGUGAGCGCCGGCCCGACAAUCAAACAGUGGGAUACACACGUAGACGCACGCUGGACGGACCUUUGGACGGCGGUUCGACUCCGCCCACCUCCACCA SEQ ID NO：30
脱硫脱硫弧菌模式种(Desulfovibrio desulfuricans)ssrA，内部部分 
GGGACTGGAACCGTAGCGGCAGGTCGAGGCGCCGCTGGCCTCGTAAAAAGCGGCACAAAAGTAATTGCCAACAACGATTACGACTACGCTTACGCTGCCTAATAACAGCGAGGCAATGACCGTTTAACGGTCGCGCCGATCAGGGCCATGCCTGATAACCCTGATTGGCGACACTTATCAGGCTGGCGAAAACCGGCTCTCGCCGGGGTTTTTCGCGAGGAGTTTACCGGCGGGATTGCTGCGTTGTGCCTGGTCAGGGGCCAACAGCGCGGTGAAATACATACTTGACCTAAACCTGTAATGCTTCGTGTG GAATGTTCTCGGACGGGG SEQ ID NO：31
脱硫脱硫弧菌模式种(Desulfovibrio desulfuricans)tmRNA，内部部分 
GGGACUGGAACCGUAGCGGCAGGUCGAGGCGCCGCUGGCCUCGUAAAAAGCGGCACAAAAGUAAUUGCCAACAACGAUUACGACUACGCUUACGCUGCCUAAUAACAGCGAGGCAAUGACCGUUUAACGGUCGCGCCGAUCAGGGCCAUGCCUGAUAACCCUGAUUGGCGACACUUAUCAGGCUGGCGAAAACCGGCUCUCGCCGGGGUUUUUCGCGAGGAGUUUACCGGCGGGAUUGCUGCGUUGUGCCUGGUCAGGGGCCAACAGCGCGGUGAAAUACAUACUUGACCUAAACCUGUAAUGCUUCGUGUGGAAUGUUCUCGGACGGGG SEQ ID NO：32
节瘤腐蹄杆菌(Dichelobacter nodosus)ssrA，3′部分 
CTCGAGGTGCATGTCGAGAATGAGAGAATCTCGTTAAATACTTTCAAAACTTATAGTTGCAAACGACGACAACTACGCTTTAGCGGCTTAATTCCCGCTTTCGCTTACCTAGATTTGTCTGTGGGTTTACCGTAAGCGACATTAACACAGAATCGCTGGTTAACGCGTCCGCTGTTAATCGGTTAAATTAAGCGGAATCGCTTGTAAAATGCCTGAGCGTTGGCTGTTTATGAGTTAAACCTAATTAACTGCTCTAAACATGTAGTACCAAAAGTTAAGGATTCGCGGACGGGGGTTCAAATCCCCCCGCCTCCACCA SEQ ID NO：33
节瘤腐蹄杆菌(Dichelobacter nodosus)tmRNA，3′部分 
CUCGAGGUGCAUGUCGAGAAUGAGAGAAUCUCGUUAAAUACUUUCAAAACUUAUAGUUGCAAACGACGACAACUACGCUUUAGCGGCUUAAUUCCCGCUUUCGCUUACCUAGAUUUGUCUGUGGGUUUACCGUAAGCGACAUUAACACAGAAUCGCUGGUUAACGCGUCCGCUGUUAAUCGGUUAAAUUAAGCGGAAUCGCUUGUAAAAUGCCUGAGCGUUGGCUGUUUAUGAGUUAAACCUAAUUAACUGCUCUAAACAUGUAGUACCAAAAGUUAAGGAUUCGCGGACGGGGGUUCAAA UCCCCCCGCCUCCACCA SEQ ID NO：34
肠球菌模式种(Enterococcus faecalis)ssrA 
GGGGGCGTTACGGATTCGACAGGCATAGTTGAGCTTGAATTGCGTTTCGTAGGTTACGGCTACGTTAAAACGTTACAGTTAAATATAACTGCTAAAAACGAAAACAATTCTTTCGCTTTAGCTGCCTAAAAACCAGCTAGCGAAGATCCTCCCGGCATCGCCCATGTGCTCGGGTCAGGGTCCTAATCGAAGTGGGATACGCTAAATTTTTCCGTCTGTAAAATTTAGAGGAGCTTACCAGACTAGCAATACAGAATGCCTGTCACTCGGCACGCTGTAAAGCGAACCTTTAAATGAGTGTCTATGAACGTAGAGATTTAAGTGGCAATATGTTTGGACGCGGGTTCGACTCCCGCCGTCTCCACCA SEQ ID NO：35
肠球菌模式种(Enterococcus faecalis)tmRNA 
GGGGGCGUUACGGAUUCGACAGGCAUAGUUGAGCUUGAAUUGCGUUUCGUAGGUUACGGCUACGUUAAAACGUUACAGUUAAAUAUAACUGCUAAAAACGAAAACAAUUCUUUCGCUUUAGCUGCCUAAAAACCAGCUAGCGAAGAUCCUCCCGGCAUCGCCCAUGUGCUCGGGUCAGGGUCCUAAUCGAAGUGGGAUACGCUAAAUUUUUCCGUCUGUAAAAUUUAGAGGAGCUUACCAGACUAGCAAUACAGAAUGCCUGUCACUCGGCACGCUGUAAAGCGAACCUUUAAAUGAGUGUCUAUGAACGUAGAGAUUUAAGUGGCAAUAUGUUUGGACGCGGGUUCGACUCCCGCCGUCUCCACCA SEQ ID NO：36
大肠杆菌(Escherichia coli)ssrA 
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTT GAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA SEQ ID NO：37
大肠杆菌(Escherichia coli)tmRNA 
GGGGCUGAUUCUGGAUUCGACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAAGCCCUGCCUGGGGUUGAAGCGUUAAAACUUAAUCAGGCUAGUUUGUUAGUGGCGUGUCCGUCCGCAGCUGGCAAGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGGACGCGGGUUCAACUCCCGCCAGCUCCACCA SEQ ID NO：38
流感嗜血杆菌(Haemophilus influenzae)ssrA 
GGGGCTGATTCTGGATTCGACGGGATTAGCGAAGCCCAAGGTGCACGTCGAGGTGCGGTAGGCCTCGTAAATAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCATTTAGCCTTCGCGCTCCAGCTTCCGCTCGTAAGACGGGGATAACGCGGAGTCAAACCAAAACGAGATCGTGTGGAAGCCACCGTTTGAGGATCGAAGCACTAAATTGAATCAAACTAGCTTAAGTTTAGCGTGTCTGTCCGCATGCTTAAGTGAAATTAAAGACGAGACTAAACGTGTAGTACTGAAGGTAGAGTAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA SEQ ID NO：39
流感嗜血杆菌(Haemophilus influenzae)tmRNA 
GGGGCUGAUUCUGGAUUCGACGGGAUUAGCGAAGCCCAAGGUGCACGUCGAGGUGCGGUAGGCCUCGUAAAUAAACCGCAAAAAAA UAGUCGCAAACGACGAACAAUACGCUUUAGCAGCUUAAUAACCUGCAUUUAGCCUUCGCGCUCCAGCUUCCGCUCGUAAGACGGGGAUAACGCGGAGUCAAACCAAAACGAGAUCGUGUGGAAGCCACCGUUUGAGGAUCGAAGCACUAAAUUGAAUCAAACUAGCUUAAGUUUAGCGUGUCUGUCCGCAUGCUUAAGUGAAAUUAAAGACGAGACUAAACGUGUAGUACUGAAGGUAGAGUAAUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA SEQ ID NO：40
幽门螺杆菌(Helicobacter pylori)(ATCC 43504)ssrA，内部部分 
AGATTTCTTGTCGCGCAGATAGCATGCCAAGCGCTGCTTGTAAAACAGCAACAAAAATAACTGTAAACAACACAGATTACGCTCCAGCTTACGCTAAAGCTGCGTGAGTTAATCTCCTTTTGGAGCTGGACTGATTAGAATTTCTAGCGTTTTAATCGCTCCATAACCTTAAGCTAGACGCTTTTAAAAGGTGGTTCGCCTTTTAAACTAAGAAACAAGAACTCTTGAAACTATCTTAAGGTTTTAGAAAGTTGGACCAGAGCTAGTTTTAAGGCTAAAAACTAACCAATTTTCTAAGCATTGTAGAAGTTTGTGTTTAGGGCAAGATTTTTGGACTGGG SEQ ID NO：41
幽门螺杆菌(Helicobacter pylori)(ATCC 43504)tmRNA，内部部分 
AGAUUUCUUGUCGCGCAGAUAGCAUGCCAAGCGCUGCUUGUAAAACAGCAACAAAAAUAACUGUAAACAACACAGAUUACGCUCCAGCUUACGCUAAAGCUGCGUGAGUUAAUCUCCUUUUGGAGCUGGACUGAUUAGAAUUUCUAGCGUUUUAAUCGCUCCAUAACCUUAAGCUAGACGCUUUUAAAAGGUGGUUCGCCUUUUAAACUAAGAAACAAGAACUCUUGAAACUAUCUUAAGGUUUUAGAAAGUUGGACCAGAGCUAGUUUUAAGGCUAAAAACUAACCAAUUUUCUAAGCAUUGUAGAAGUUUGUGUUUAGGGCAAGAUUUUUGGACUGGGSEQ ID NO：42
幽门螺杆菌(Helicobacter pylori)(菌株26695)ssrA 
GGGGCTGACTTGGATTTCGACAGATTTCTTGTCGCACAGATAGCATGCCAAGCGCTGCTTGTAAAACAGCAACAAAAATAACTGTAAACAACACAGATTACGCTCCAGCTTACGCTAAAGCTGCGTGAGTTAATCTCCTTTTGGAGCTGGACTGATTAGAATTTCTAGCGTTTTAATCGCTCCATAACCTTAAGCTAGACGCTTTTAAAAGGTGGTTCGCCTTTTAAACTAAGAAACAAGAACTCTTGAAACTATCTCAAGGTTTTAGAAAGTTGGACCAGAGCTAGTTTTAAGGCTAAAAAACCAACCAATTTTCTAAGCATTGTAGAAGTTTGTGTTTAGGGCAAGATTTTTGGACTGGGGTTCGATTCCCCACAGCTCCACCA SEQ ID NO：43
幽门螺杆菌(Helicobacter pylori)(菌株26695)tmRNA 
GGGGCUGACUUGGAUUUCGACAGAUUUCUUGUCGCACAGAUAGCAUGCCAAGCGCUGCUUGUAAAACAGCAACAAAAAUAACUGUAAACAACACAGAUUACGCUCCAGCUUACGCUAAAGCUGCGUGAGUUAAUCUCCUUUUGGAGCUGGACUGAUUAGAAUUUCUAGCGUUUUAAUCGCUCCAUAACCUUAAGCUAGACGCUUUUAAAAGGUGGUUCGCCUUUUAAACUAAGAAACAAGAACUCUUGAAACUAUCUCAAGGUUUUAGAAAGUUGGACCAGAGCUAGUUUUAAGGCUAAAAAACCAACCAAUUUUCUAAGCAUUGUAGAAGUUUGUGUUUAGGGCAAGAUUUUUGGACUGGGGUUCGAUUCCCCACAGCUCCACCASEQ ID NO：44
产气克雷伯氏菌(Klebsiella aerogenes)(NCTC 9528)ssrA，内部部分 
GGGATTCGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTAAGAGCCCTCTCTCCCTAGCTTCCGCTCCTAAGACGGGGAATAAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTAATCAGGCTAGTTTGTCAGTGGCGTGTCCGTCCGCAGCTGGCCAGCGAATGTAAAGACTGGACTAAGCATGTAGTGCCGAGGATGTAGGAATTT C SEQ ID NO：45 
产气克雷伯氏菌(Klebsiella aerogenes)(NCTC 9528)tmRNA，内部部分 
GGGAUUCGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUAAGAGCCCUCUCUCCCUAGCUUCCGCUCCUAAGACGGGGAAUAAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAAGCCCUGCCUGGGGUUGAAGCGUUAAAACUAAUCAGGCUAGUUUGUCAGUGGCGUGUCCGUCCGCAGCUGGCCAGCGAAUGUAAAGACUGGACUAAGCAUGUAGUGCCGAGGAUGUAGGAAUUUC SEQ ID NO：46
乳杆菌模式种(Lactobacillus lactis)(NCTC 662)ssrA，内部部分 
AAGCACAGTTCGAGCTTGAATTGCGTTTCGTAGGTTACGTCTACGTTAAAACGTTACAGTTAAATATAACTGCTAAAAACGAAAACAACTCTTACGCTTTAGCTGCCTAAAAACAGTTAGCGTAGATCCTCTCGGCATCGCCCATGTGCTCGAGTAAGGGTCTCAAATTTAGTGGGATACGTTAAACTTTTCCGTCTGTAAAGTTTAAAAGAGATCATCAGACTAGCGATACAGAATGCCTGTCACTCGGCAAGCTGTAAAGCGAAACCTCAAATGAGTTGACTATGAACGTAGATTTTTAAGTGTCGATGTGTTT SEQ ID NO：47
乳杆菌模式种(Lactobacillus lactis)(NCTC 662)tmRNA，内部部分 
AAGCACAGUUCGAGCUUGAAUUGCGUUUCGUAGGUUACGUCUACGUUAAAACGUUACAGUUAAAUAUAACUGCUAAAAACGAAAACAACUCUUACGCUUUAGCUGCCUAAAAACAGUUAGCGUAGAUCCUCUCGGCAUCGCCCAUGUGCUCGAGUAAGGGUCUCAAAUUUAGUGGGAUACGUUAAACUUUUCCGUCUGUAAAGUUUAAAAGAGAUCAUCAGACUAGCGAUACAGAAUGCCUGUCACUCGGCAAGCUGU AAAGCGAAACCUCAAAUGAGUUGACUAUGAACGUAGAUUUUUAAGUGUCGAUGUGUUU SEQ ID NO：48
嗜肺军团菌模式种(Legionella pneumophila)ssrA，内部部分 
GTGGGTTGCAAAACCGGAAGTGCATGCCGAGAAGGAGATCTCTCGTAAATAAGACTCAATTAAATATAAATGCAAACGATGAAAACTTTGCTGGTGGGGAAGCTATCGCTGCCTAATAAGCACTTTAGTTAAACCATCACTGTGTACTGGCCAATAAACCCAGTATCCCGTTCGACCGAGCCCGCTTATCGGTATCGAATCAACGGTCATAAGAGATAAGCTAGCGTCCTAATCTATCCCGGGTTATGGCGCGAAACTCAGGGAATCGCTGTGTATCATCCTGCCCGTCGGAGGAGCCACAGTTAAATTCAAAAGACAAGGCTATGCATGTAGAGCTAAAGGCAGAGGACTTGCGGACGCGG SEQ ID NO：49
嗜肺军团菌模式种(Legionella pneumophila)tmRNA，内部部分 
GUGGGUUGCAAAACCGGAAGUGCAUGCCGAGAAGGAGAUCUCUCGUAAAUAAGACUCAAUUAAAUAUAAAUGCAAACGAUGAAAACUUUGCUGGUGGGGAAGCUAUCGCUGCCUAAUAAGCACUUUAGUUAAACCAUCACUGUGUACUGGCCAAUAAACCCAGUAUCCCGUUCGACCGAGCCCGCUUAUCGGUAUCGAAUCAACGGUCAUAAGAGAUAAGCUAGCGUCCUAAUCUAUCCCGGGUUAUGGCGCGAAACUCAGGGAAUCGCUGUGUAUCAUCCUGCCCGUCGGAGGAGCCACAGUUAAAUUCAAAAGACAAGGCUAUGCAUGUAGAGCUAAAGGCAGAGGACUUGCGGACGCGG SEQ ID NO：50
格氏李斯特氏菌(Listeria grayi)ssrA，内部部分 
ACAGGGATAGGTCGAGCTTGAGTTGCGAGCCGGGGGGATCGGCCCGTCATCAACGTCAAAGCCAATAATAACTGGCAAACAAAACAACAATTTAGCTTTCGCTGCCTAATAGCAGTCTGAATAGCTGATCCTCCGTGCATCACCCATGTGCTACGGTAAGGGTCTCACTTTTAAGTGG GTTACGCTGGCTTATCTCCGTCTGGGGCAAACGAGAAGAGCATAATCAGACTAGCTAGATAGAGCCCTGACGCCGGGCAGACATCTATGCGAAATCCAAATACGGCAACTACGCTCGTAGATGCTCAAGTGCCGATATTTCTGG SEQ ID NO：51
格氏李斯特氏菌(Listeria grayi)tmRNA，内部部分 
ACAGGGAUAGGUCGAGCUUGAGUUGCGAGCCGGGGGGAUCGGCCCGUCAUCAACGUCAAAGCCAAUAAUAACUGGCAAACAAAACAACAAUUUAGCUUUCGCUGCCUAAUAGCAGUCUGAAUAGCUGAUCCUCCGUGCAUCACCCAUGUGCUACGGUAAGGGUCUCACUUUUAAGUGGGUUACGCUGGCUUAUCUCCGUCUGGGGCAAACGAGAAGAGCAUAAUCAGACUAGCUAGAUAGAGCCCUGACGCCGGGCAGACAUCUAUGCGAAAUCCAAAUACGGCAACUACGCUCGUAGAUGCUCAAGUGCCGAUAUUUCUGG SEQ ID NO：52
无害李斯特氏菌(Listeria innocua)ssrA，内部部分 
ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAAGTGCCGATATTTCTGG SEQ ID NO：53
无害李斯特氏菌(Listeria innocua)tmRNA，内部部分 
ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCU AAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAAGUGCCGAUAUUUCUGG SEQ ID NO：54
单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 7973)ssrA，内部部分 
ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG SEQ ID NO：55
单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC7973)tmRNA，内部部分 
ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG SEQ ID NO：56
单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 11994)ssrA，内部部分 
CAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTC GCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTT SEQ ID NO：57
单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 11994)tmRNA，内部部分 
CAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUSEQ ID NO：58
墨氏李斯特氏菌(Listeria murrayi)ssrA，内部部分 
ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATGAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAAGTGCCGATATTTCTGG SEQ ID NO：59
墨氏李斯特氏菌(Listeria murrayi)tmRNA，内部部分 
ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGA UCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUGAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAAGUGCCGAUAUUUCUGG SEQ ID NO：60
威氏李斯特氏菌(Listeria welshimeri)ssrA，内部部分 
ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTGGCTAATCTCCGTCTGAGGTTAGTTGGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG SEQ ID NO：61
威氏李斯特氏菌(Listeria welshimeri)tmRNA，内部部分 
ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUGGCUAAUCUCCGUCUGAGGUUAGUUGGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG SEQ ID NO：62
除烃海杆菌(Marinobacter hydrocarbonoclasticus)ssrA，内部部分 
GCCGGTGACGAACCCTTGGGTGCATGCCGAGATGGCAGCGAATCTCGTAAATCCAAAGCTGCAACGTAATAGTCGCAAACGACGAAAACTACGCACTGGCGGCGTAAGCCGTTCCAGTCGTCCTGGCTGAGGC GCCTATAACTCAGTAGCAACATCCCAGGACGTCATCGCTTATAGGCTGCTCCGTTCACCAGAGCTCACTGGTGTTCGGCTAAGATTAAAGAGCTCGCCTCTTGCACCCTGACCTTCGGGTCGCTTGAGGTTAAATCAATAGAAGGACACTAAGCATGTAGACCTCAAGGCCTAGTGCTGGCGGACGCGG SEQ ID NO：63
除烃海杆菌(Marinobacter hydrocarbonoclasticus)tmRNA，内部部分 
GCCGGUGACGAACCCUUGGGUGCAUGCCGAGAUGGCAGCGAAUCUCGUAAAUCCAAAGCUGCAACGUAAUAGUCGCAAACGACGAAAACUACGCACUGGCGGCGUAAGCCGUUCCAGUCGUCCUGGCUGAGGCGCCUAUAACUCAGUAGCAACAUCCCAGGACGUCAUCGCUUAUAGGCUGCUCCGUUCACCAGAGCUCACUGGUGUUCGGCUAAGAUUAAAGAGCUCGCCUCUUGCACCCUGACCUUCGGGUCGCUUGAGGUUAAAUCAAUAGAAGGACACUAAGCAUGUAGACCUCAAGGCCUAGUGCUGGCGGACGCGG SEQ ID NO：64
鸟分枝杆菌(Mycobacterium avium)ssrA，内部部分 
TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACTGACCACCGTAAGCGTCGTTGCAAATAGATAAGCGCCGATTCACATCAGCGCGACTTACCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGCCCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTTGTTCGCGTGACCAGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGAACCCGGGTTCGATTCCCAA SEQ ID NO：65
鸟分枝杆菌(Mycobacterium avium)tmRNA，内部部分 
UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACUGACCACCGUAAGCGUCGUUGCAAAUAGAUAAGCGCCGAUUCACAUCAGCGCGACUUACCUCUCGCUGCCUAAGCGACAGCUAGUCCG UCAGCCCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUUGUUCGCGUGACCAGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGAACCCGGGUUCGAUUCCCAASEQ ID NO：66
牛分枝杆菌(Mycobacterium bovis)ssrA，内部部分 
TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGG SEQ ID NO：67
牛分枝杆菌(Mycobacterium bovis)tmRNA，内部部分 
UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGG SEQ ID NO：68
麻风分枝杆菌(Mycobacterium leprae)ssrA 
GGGGCTGAAAGGTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAGCAAT ATAAGCGCCGATTCATATCAGCGCGACTATGCTCTCGCTGCCTAAGCGATGGCTAGTCTGTCAGACCGGGAACGCCCTCGTCCCGGAGCCTGGCATCAGCTAGAGGGATCTACCGATGGGTTCGGTCGCGGGACTCGTCGGGACACCAACCGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGATCAGGAGATCCGAGTAGAGGCATAGCGAACTACGCACGGAGAAGCCTTGAGGGAAATGCCGTAGGACCCGGGTTCGATTCCCGGCAGCTCCACCA SEQ ID NO：69
麻风分枝杆菌(Mycobacterium leprae)tmRNA 
GGGGCUGAAAGGUUUCGACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAGCAAUAUAAGCGCCGAUUCAUAUCAGCGCGACUAUGCUCUCGCUGCCUAAGCGAUGGCUAGUCUGUCAGACCGGGAACGCCCUCGUCCCGGAGCCUGGCAUCAGCUAGAGGGAUCUACCGAUGGGUUCGGUCGCGGGACUCGUCGGGACACCAACCGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGAUCAGGAGAUCCGAGUAGAGGCAUAGCGAACUACGCACGGAGAAGCCUUGAGGGAAAUGCCGUAGGACCCGGGUUCGAUUCCCGGCAGCUCCACCA SEQ ID NO：70
鸟分枝杆菌(副结核亚种)(Mycobacterium paratuberculosis)ssrA，内部部分 
TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACTGACCACCGTAAGCGTCGTTGCAAATAGATAAGCGCCGATTCACATCAGCGCGACTTACCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGCCCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTTGTTCGCGTGACCAGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGAACCCGGGTTCGATTCCCAA SEQ ID NO：71 
鸟分枝杆菌(副结核亚种)(Mycobacterium paratuberculosis)tmRNA，内部部分 
UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACUGACCACCGUAAGCGUCGUUGCAAAUAGAUAAGCGCCGAUUCACAUCAGCGCGACUUACCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGCCCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUUGUUCGCGUGACCAGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGAACCCGGGUUCGAUUCCCAASEQ ID NO：72
结核分枝杆菌(Mycobacterium tuberculosis)ssrA
GGGGCTGAACGGTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGGACCCGGGTTCGATTCCCGGCAGCTCCACCA SEQ ID NO：73
结核分枝杆菌(Mycobacterium tuberculosis)tmRNA 
GGGGCUGAACGGUUUCGACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCU CGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGGACCCGGGUUCGAUUCCCGGCAGCUCCACCA SEQ ID NO：74
山羊枝原体(Mycoplasma capricolum)ssrA 
GGGGATGTCATGGATTTGACAGGATATCTTTAGTACATATAAGCAGTAGTGTTGTAGACTATAAATACTACTAGGTTTAAAAAAACGCAAATAAAAACGAAGAAACTTTTGAAATGCCAGCATTTATGATGAATAATGCATCAGCTGGAGCAAACTTTATGTTTGCTTAATAACTACTAGTTTAGTTATAGTATTTCACGAATTATAGATATTTTAAGCTTTATTTATAACCGTATTACCCAAGCTTAATAGAATATATGATTGCAATAAATATATTTGAAATCTAATTGCAAATGATATTTAACCTTTAGTTAATTTTAGTTAAATATTTTAATTAGAAAATTAACTAAACTGTAGAAAGTATGTATTAATATATCTTGGACGCGAGTTCGATTCTCGCCATCTCCACCA SEQ ID NO：75
山羊枝原体(Mycoplasma capricolum)tmRNA 
GGGGAUGUCAUGGAUUUGACAGGAUAUCUUUAGUACAUAUAAGCAGUAGUGUUGUAGACUAUAAAUACUACUAGGUUUAAAAAAACGCAAAUAAAAACGAAGAAACUUUUGAAAUGCCAGCAUUUAUGAUGAAUAAUGCAUCAGCUGGAGCAAACUUUAUGUUUGCUUAAUAACUACUAGUUUAGUUAUAGUAUUUCACGAAUUAUAGAUAUUUUAAGCUUUAUUUAUAACCGUAUUACCCAAGCUUAAUAGAAUAUAUGAUUGCAAUAAAUAUAUUUGAAAUCUAAUUGCAAAUGAUAUUUAACCUUUAGUUAAUUUUAGUUAAAUAUUUUAAUUAGAAAAUUAACUAAACUGUAGAAAGUAUGUAUUAAUAUAUCUUGGACGCGAGUUCGAUUCUCGCCAUCUCCACCA SEQ ID NO：76
生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#1)ssrA 
GGGGATGTTTTGGGTTTGACATAATGCTGATAGACAAACAGTAGC ATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGAACCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA SEQ ID NO：77
生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#1)tmRNA 
GGGGAUGUUUUGGGUUUGACAUAAUGCUGAUAGACAAACAGUAGCAUUGGGGUAUGCCCCUUACAGCGCUAGGUUCAAUAACCGACAAAGAAAAUAACGAAGUGUUGGUAGAACCAAAUUUGAUCAUUAACCAACAAGCAAGUGUUAACUUUGCUUUUGCAUAAGUAGAUACUAAAGCUACAGCUGGUGAAUAGUCAUAGUUUGCUAGCUGUCAUAGUUUAUGACUCGAGGUUAAAUCGUUCAAUUUAACCUUUAAAAAUAGAACUUGUUGUUUCCAUGAUUGUUUUGUGAUCAAUUGGAAACAAGACAAAAAUCCACAAAACUAAAAUGUAGAAGCUGUUUGUUGUGUCCUUUAUGGAAACGGGUUCGAUUCCCGUCAUCUCCACCA SEQ ID NO：78
生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#2)tmRNA，内部部分 
ACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTA GAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCSEQ ID NO：79
生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#2)tmRNA，内部部分 
ACAUAAUGCUGAUAGACAAACAGUAGCAUUGGGGUAUGCCCCUUACAGCGCUAGGUUCAAUAACCGACAAAGAAAAUAACGAAGUGUUGGUAGAUCCAAAUUUGAUCAUUAACCAACAAGCAAGUGUUAACUUUGCUUUUGCAUAAGUAGAUACUAAAGCUACAGCUGGUGAAUAGUCAUAGUUUGCUAGCUGUCAUAGUUUAUGACUCGAGGUUAAAUCGUUCAAUUUAACCUUUAAAAAUAGAACUUGUUGUUUCCAUGAUUGUUUUGUGAUCAAUUGGAAACAAGACAAAAAUCCACAAAACUAAAAUGUAGAAGCUGUUUGUUGUGUCCUUUAUGGAAACGGGUUC SEQ ID NO：80
嗜肺枝原体(Mycoplasma pneumophila)ssrA 
GGGGATGTAGAGGTTTTGACATAATGTTGAAAGGAAAACAGTTGCAGTGGGGTATGCCCCTTACAGCTCTAGGTATAATAACCGACAAAAATAACGACGAAGTTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAGAACATACTAAAGCTACACGAATTGAATCGCCATAGTTTGGTTCGTGTCACAGTTTATGGCTCGGGGTTAACTGGTTCAACTTAATCCTTAAATTATGAACTTATCGTTTACTTGTTTGTCTTATGATCTAAAGTAAGCGAGACATTAAAACATAAGACTAAACTGTAGAAGCTGTTTTACCAATCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA SEQ ID NO：81
嗜肺枝原体(Mycoplasma pneumophila)tmRNA 
GGGGAUGUAGAGGUUUUGACAUAAUGUUGAAAGGAAAACAGUUGCAGUGGGGUAUGCCCCUUACAGCUCUAGGUAUAAUAACCGACAAAAAUAACGACGAAGUUUUGGUAGAUCCAAUGUUGAUCGCU AACCAACAAGCAAGUAUCAACUACGCUUUCGCUUAGAACAUACUAAAGCUACACGAAUUGAAUCGCCAUAGUUUGGUUCGUGUCACAGUUUAUGGCUCGGGGUUAACUGGUUCAACUUAAUCCUUAAAUUAUGAACUUAUC GUUUACUUGUUUGUCUUAUGAUCUAAAGUAAGCGAGACAUUAAAACAUAAGACUAAACUGUAGAAGCUGUUUUACCAAUCCUUUAUGGAAACGGGUUCGAUUCCCGUCAUCUCCACCA SEQ ID NO：82
淋病奈瑟球菌(Neisseria gonorrhoeae)(ATCC 19424)ssrA，内部部分 
GGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGCGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCAGCCGGGTTACTTGGCAGGAAATAAGACTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGG SEQ ID NO：83
淋病奈瑟球菌(Neisseria gonorrhoeae)(ATCC 19424)tmRNA，内部部分 
GGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGCGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCAGCCGGGUUACUUGGCAGGAAAUAAGACUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGG SEQ ID NO：84
淋病奈瑟球菌(Neisseria gonorrhoeae)(FA 1090)ssrA 
GGGGGCGACCTTGGTTTCGACGGGGGTTGCGAAGCAGATGCGGG CATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGTGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCAGCCGGGTTACTTGGCAGGAAATAAGACTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGGGTTCGATTCCCCCCGCCTCCACCA SEQ ID NO：85
淋病奈瑟球菌(Neisseria gonorrhoeae)(FA 1090)tmRNA 
GGGGGCGACCUUGGUUUCGACGGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGUGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCAGCCGGGUUACUUGGCAGGAAAUAAGACUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGGGUUCGAUUCCCCCCGCCUCCACCA SEQ ID NO：86
脑膜炎奈瑟氏球菌(Neisseria meningitidis)ssrA 
GGGGGCGACCTTGGTTTCGACGGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGCGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCTGCCGGGTTATTTGGCAGGAAATGAGATTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGGGTTCGATTCCCCCCGCCTCCACCA SEQ ID NO：87 
脑膜炎奈瑟氏球菌(Neisseria meningitidis)tmRNA 
GGGGGCGACCUUGGUUUCGACGGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGCGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCUGCCGGGUUAUUUGGCAGGAAAUGAGAUUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGGGUUCGAUUCCCCCCGCCUCCACCA SEQ ID NO：88
Nostoc muscorum PCC7120ssrA
GGGTCCGTCGGTTTCGACAGGTTGGCGAACGCTACTCTGTGATTCAGGTCGAGAGTGAGTCTCCTCTGCAAATCAAGGCTCAAAACAAAAGTAAATGCGAATAACATCGTTAAATTTGCTCGTAAGGACGCTCTAGTAGCTGCCTAAATAGCCTCTTTCAGGTTCGAGCGTCTTCGGTTTGACTCCGTTAAGGACTGAAGACCAACCCCCAACGGATGCTCTAGCAATGTTCTCTGGTTGGCTTGCTAGCTAAGATTTAATCAGAGCATCCTACGTTCGGGATAATGAACGATTCCCGCCTTGAGGGTCAGAAAGGCTAAACCTGTGAATGAGCGGGGGGTCAATACCCAATTTGGACAGCAGTTCGACTCTGCTCGATCCACCA SEQ ID NO：89
Nostoc muscorum PCC7120tmRNA 
GGGUCCGUCGGUUUCGACAGGUUGGCGAACGCUACUCUGUGAUUCAGGUCGAGAGUGAGUCUCCUCUGCAAAUCAAGGCUCAAAACAAAAGUAAAUGCGAAUAACAUCGUUAAAUUUGCUCGUAAGGACGCUCUAGUAGCUGCCUAAAUAGCCUCUUUCAGGUUCGAGCGUCUUCGGUUUGACUCCGUUAAGGACUGAAGACCAACCCCCAACGGAUGCUCUAGCAAUGUUCUCUGGUUGGCUUGCUAGCUAAGAUUU AAUCAGAGCAUCCUACGUUCGGGAUAAUGAACGAUUCCCGCCUUGAGGGUCAGAAAGGCUAAACCUGUGAAUGAGCGGGGGGUCAAUACCCAAUUUGGACAGCAGUUCGACUCUGCUCGAUCCACCASEQ ID NO：90
Odontella sinensis(硅藻属)叶绿体ssrA 
GGGGCTGACTTGGTTTCGACATTTAAAAATTGTTACAGTATGATGCAGGTCGAAGTTTCTAATCTTCGTAAAAAAAGAGAAATTTATAATAAATGCTAATAATTTAATTTCTTCTGTGTTTAAAAGTTTATCAACTAAGCAAAATAGTTTAAATTTAAGTTTTGCTGTTTAAGTTTTATGCACATTTAATGATCTAGTAAATAACTTTGTTCGCTATAATTTATATTTATAACTAGACTTTTGTCTTTTTTATAGTTTAGAATAACTTTATCATTTCAAACCTCGTTCCATCTAGTTGAACTAAACCTGTGAACGAATACTATAATAAAATTTTTAGATGGACGTGGGTTCGACTCCCATCAGCTCCACCA SEQ ID NO：91
Odontella sinensis(硅藻属)叶绿体tmRNA 
GGGGCUGACUUGGUUUCGACAUUUAAAAAUUGUUACAGUAUGAUGCAGGUCGAAGUUUCUAAUCUUCGUAAAAAAAGAGAAAUUUAUAAUAAAUGCUAAUAAUUUAAUUUCUUCUGUGUUUAAAAGUUUAUCAACUAAGCAAAAUAGUUUAAAUUUAAGUUUUGCUGUUUAAGUUUUAUGCACAUUUAAUGAUCUAGUAAAUAACUUUGUUCGCUAUAAUUUAUAUUUAUAACUAGACUUUUGUCUUUUUUAUAGUUUAGAAUAACUUUAUCAUUUCAAACCUCGUUCCAUCUAGUUGAACUAAACCUGUGAACGAAUACUAUAAUAAAAUUUUUAGAUGGACGUGGGUUCGACUCCCAUCAGCUCCACCASEQ ID NO：92
卟啉单胞菌(Porphyra purpureum)(红藻)叶绿体ssrA 
GGGGCTGCAAGGTTTCTACATTGTGAAAAAACAAATATATGAAA 
GTAAAACGAGCTCATTATTAGAGCTTTTAGTTAAATAAATGCAGAAAATAATATTATTGCTTTTTCTCGAAAATTAGCTGTTGCATAAATAGTCTCAATTTTTGTAATTCGAAGTGATAGACTCTTATACACTACGAATATTCTGTTAGAGTTGCTCTTAATAAAAGAAAAGTAAAAAAATACAAATTCTTATGTTTTTTACCTGAATTGATTCAATTTAAGGTTAGTATTTTTTGATTTTTACAATGGACGTGGGTTCAAGTCCCACCAGCTCCACCA SEQ ID NO：93
卟啉单胞菌(Porphyra purpureum)(红藻)叶绿体tmRNA 
GGGGCUGCAAGGUUUCUACAUUGUGAAAAAACAAAUAUAUGAAAGUAAAACGAGCUCAUUAUUAGAGCUUUUAGUUAAAUAAAUGCAGAAAAUAAUAUUAUUGCUUUUUCUCGAAAAUUAGCUGUUGCAUAAAUAGUCUCAAUUUUUGUAAUUCGAAGUGAUAGACUCUUAUACACUACGAAUAUUCUGUUAGAGUUGCUCUUAAUAAAAGAAAAGUAAAAAAAUACAAAUUCUUAUGUUUUUUACCUGAAUUGAUUCAAUUUAAGGUUAGUAUUUUUUGAUUUUUACAAUGGACGUGGGUUCAAGUCCCACCAGCUCCACCA SEQ ID NO：94
牙龈卟啉单胞菌(Porphyromonas gingivalis)ssrA 
GGGGCTGACCGGCTTTGACAGCGTGATGAAGCGGTATGTAAGCATGTAGTGCGTGGGTGGCTTGCACTATAATCTCAGACATCAAAAGTTTAATTGGCGAAAATAACTACGCTCTCGCTGCGTAATCGAAGAATAGTAGATTAGACGCTTCATCGCCGCCAAAGTGGCAGCGACGAGACATCGCCCGAGCAGCTTTTTCCCGAAGTAGCTCGATGGTGCGGTGCTGACAAATCGGGAACCGCTACAGGATGCTTCCTGCCTGTGGTCAGATCGAACGGAAGATAAGGATCGTGCATTGGGTCGTTTCAGCCTCCGCTCGCTCACGAAAATTCCAACTGAAACTAAACATGTAGAAAGCATATTGATTCCATGTTTGGACGAGGGTTCAATTCCCTCCAGCTCCACCA SEQ ID NO：95 
牙龈卟啉单胞菌(Porphyromonas gingivalis)tmRNAGGGGCUGACCGGCUUUGACAGCGUGAUGAAGCGGUAUGUAAGCAUGUAGUGCGUGGGUGGCUUGCACUAUAAUCUCAGACAUCAAAAGUUUAAUUGGCGAAAAUAACUACGCUCUCGCUGCGUAAUCGAAGAAUAGUAGAUUAGACGCUUCAUCGCCGCCAAAGUGGCAGCGACGAGACAUCGCCCGAGCAGCUUUUUCCCGAAGUAGCUCGAUGGUGCGGUGCUGACAAAUCGGGAACCGCUACAGGAUGCUUCCUGCCUGUGGUCAGAUCGAACGGAAGAUAAGGAUCGUGCAUUGGGUCGUUUCAGCCUCCGCUCGCUCACGAAAAUUCCAACUGAAACUAAACAUGUAGAAAGCAUAUUGAUUCCAUGUUUGGACGAGGGUUCAAUUCCCUCCAGCUCCACCA SEQ ID NO：96
雷氏变性菌(Proteus rettgeri)ssrA(NCTC 10975)，内部部分 
GGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGATGCCTTGCCTGGGGTTGAAGCGTTAAACTTAATCAGGATAGTTTGTTGGTGGCGTGTCTGTCCGCAGCTGGCAAATGAATTCAAAGACTAGACTAAGCATGTAGTACCGAGGATGTAGAAATTTCSEQ ID NO：97
雷氏变性菌(Proteus rettgeri)ssrA tmRNA(NCTC 10975)，内部部分 
GGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAUGCCUUGCCUGGGGUUGAAGCGUUAAACUUAAUCAGGAUAGUUUGUUGGUGGCGUGUCUGUCCGCAGCUGGCAAAUGAAUUCAAAGACUAGACUAAGCAUGUAGUACCGAGGAUGU AGAAAUUUC SEQ ID NO：98
游海假交替单胞菌(Pseudoalteromonas haloplanktoni)ssrA，内部部分 
GGAATTCAAGAAGCCCGAGGTGCATGTCGAGGTGCGGTTTGCCTCGTAAAAAAGCCGCAATTTAAAGTAATCGCAAACGACGATAACTACTCTCTAGCAGCTTAGGCTGGCTAGCGCTCCTTCCATGTATTCTTGTGGACTGGATTTTGGAGTGTCACCCTAACACCTGATCGCGACGGAAACCCTGGCCGGGGTTGAAGCGTTAAAACTAAGCGGCCTCGCCTTTATCTACCGTGTTTGTCCGGGATTTAAAGGTTAATTAAATGACAATACTAAACATGTAGTACCGACGGTCGAGGCTTTTCGGACGGGG SEQ ID NO：99
游海假交替单胞菌(Pseudoalteromonas haloplanktoni)tmRNA，内部部分 
GGAAUUCAAGAAGCCCGAGGUGCAUGUCGAGGUGCGGUUUGCCUCGUAAAAAAGCCGCAAUUUAAAGUAAUCGCAAACGACGAUAACUACUCUCUAGCAGCUUAGGCUGGCUAGCGCUCCUUCCAUGUAUUCUUGUGGACUGGAUUUUGGAGUGUCACCCUAACACCUGAUCGCGACGGAAACCCUGGCCGGGGUUGAAGCGUUAAAACUAAGCGGCCUCGCCUUUAUCUACCGUGUUUGUCCGGGAUUUAAAGGUUAAUUAAAUGACAAUACUAAACAUGUAGUACCGACGGUCGAGGCUUUUCGGACGGGG SEQ ID NO：100 
铜绿假单胞菌(Pseudomonas aeruginosa)ssrA 
GGGGCCGATTAGGATTCGACGCCGGTAACAAAAGTTGAGGGGCATGCCGAGTTGGTAGCAGAACTCGTAAATTCGCTGCTGCAAACTTATAGTTGCCAACGACGACAACTACGCTCTAGCTGCTTAATGCGGCTAGCAGTCGCTAGGGGATGCCTGTAAACCCGAAACGACTGTCAGATAGAACAGGATCGCCGCCAAGTTCGCTGTAGACGTAACGGCTAAAACTCATACAGCTCGCTCCAAGCACCCTGCCACTCGGGCGGCGCG GAGTTAACTCAGTAGAGCTGGCTAAGCATGTAAAACCGATAGCGGAAAGCTGGCGGACGGGGGTTCAAATCCCCCCGGTTCCACCASEQ ID NO：101 
铜绿假单胞菌(Pseudomonas aeruginosa)tmRNA 
GGGGCCGAUUAGGAUUCGACGCCGGUAACAAAAGUUGAGGGGCAUGCCGAGUUGGUAGCAGAACUCGUAAAUUCGCUGCUGCAAACUUAUAGUUGCCAACGACGACAACUACGCUCUAGCUGCUUAAUGCGGCUAGCAGUCGCUAGGGGAUGCCUGUAAACCCGAAACGACUGUCAGAUAGAACAGGAUCGCCGCCAAGUUCGCUGUAGACGUAACGGCUAAAACUCAUACAGCUCGCUCCAAGCACCCUGCCACUCGGGCGGCGCGGAGUUAACUCAGUAGAGCUGGCUAAGCAUGUAAAACCGAUAGCGGAAAGCUGGCGGACGGGGGUUCAAAUCCCCCCGGUUCCACCA SEQ ID NO：102 
鼠伤寒沙门氏菌(Salmonella typhimurium)ssrA 
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAACCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGCGGATGCCCTGCCTGGGGTTGAAGCGTTAAAACGAATCAGGCTAGTCTGGTAGTGGCGTGTCCGTCCGCAGGTGCCAGGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA SEQ ID NO：103 
鼠伤寒沙门氏菌(Salmonella typhimurium)tmRNA 
GGGGCUGAUUCUGGAUUCGACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAACCUACGCUUUAGCAGCUUAAUAACCU GCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGCGGAUGCCCUGCCUGGGGUUGAAGCGUUAAAACGAAUCAGGCUAGUCUGGUAGUGGCGUGUCCGUCCGCAGGUGCCAGGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGGACGCGGGUUCAACUCCCGCCAGCUCCACCA SEQ ID NO：104 
希瓦氏菌模式种(Shewanella putrefaciens)ssrA 
GGGGGCGATTCTGGATTCGACAGGATTCACGAAACCCTGGGAGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAGTTATAGTTGCAAACGACGATAACTACGCTCTAGCCGCTTAATGCCGCTAGCCATCTACCACACGCTTTGCACATGGGCAGTGGATTTGATGGTCATCTCACATCGTGCTAGCGAGGGAACCCTGTCTGGGGGTGAACCGCGAAACAGTACCGGACTCACCGTGTGGGATCCTGTCTTTCGGAGTTCAAACGGTTAAACAATAGAAAGACTAAGCATGTAGCGCCTTGGATGTAGGTTTTCTGGACGCGGGTTCAAGTCCCGCCGCCTCCACCASEQ ID NO：105 
希瓦氏菌模式种(Shewanella putrefaciens)tmRNA 
GGGGGCGAUUCUGGAUUCGACAGGAUUCACGAAACCCUGGGAGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAGUUAUAGUUGCAAACGACGAUAACUACGCUCUAGCCGCUUAAUGCCGCUAGCCAUCUACCACACGCUUUGCACAUGGGCAGUGGAUUUGAUGGUCAUCUCACAUCGUGCUAGCGAGGGAACCCUGUCUGGGGGUGAACCGCGAAACAGUACCGGACUCACCGUGUGGGAUCCUGUCUUUCGGAGUUCAAACGGUUAAACAAUAGAAAGACUAAGCAUGUAGCGCCUUGGAUGUAGGUUUUCUGGACGCGGGUUCAAGUCCCGCCGCCUCCACCA SEQ ID NO：106 
金黄色葡萄球菌(Staphylococcus aureus)ssrA 
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTCGGAGGGTTGTCTTCGTCATCAACACACACAGTTTATAATAACTGGCAAATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAGCATTTCCTATGTGCTGTTAACGCGATTCAACCTTAATAGGATATGCTAAACACTGCCGTTTGAAGTCTGTTTAGAAGAAACTTAATCAAACTAGCATCATGTTGGTTGTTTATCACTTTTCATGATGCGAAACCTATCGATAAACTACACACGTAGAAAGATGTGTATCAGGACCTTTGGACGCGGGTTCAAATCCCGCCGTCTCCACCA SEQ ID NO：107 
金黄色葡萄球菌(Staphylococcus aureus)tmRNA 
GGGGACGUUCAUGGAUUCGACAGGGGUCCCCCGAGCUCAUUAAGCGUGUCGGAGGGUUGUCUUCGUCAUCAACACACACAGUUUAUAAUAACUGGCAAAUCAAACAAUAAUUUCGCAGUAGCUGCCUAAUCGCACUCUGCAUCGCCUAACAGCAUUUCCUAUGUGCUGUUAACGCGAUUCAACCUUAAUAGGAUAUGCUAAACACUGCCGUUUGAAGUCUGUUUAGAAGAAACUUAAUCAAACUAGCAUCAUGUUGGUUGUUUAUCACUUUUCAUGAUGCGAAACCUAUCGAUAAACUACACACGUAGAAAGAUGUGUAUCAGGACCUUUGGACGCGGGUUCAAAUCCCGCCGUCUCCACCA SEQ ID NO：108 
戈氏链球菌(Streptococcus gordonii)ssrA 
GGGGTCGTTACGGATTCGACAGGCATTATGAGGCATATTTTGCGACTCATCTAGCGGATGTAAAACGCCAGTTAAATATAACTGCAAAAAATAATACTTCTTACGCTTTAGCTGCCTAAAAACCAGCGGGCGTGACCCGATTCGGATTGCTTGTGTCTGATGACAGGTCTTATTATTAGCAAGCTACGGTAGAATCTTGTCTAGTGATTTTACAAGAGATTGATAGACTCGCTTGATTTGGGCTTGAGTTATGTGTCAAAATCAAGTTAAAACAATACATAGCCTATGGTTGTAGACAAATGTGTTGGCAGATGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCA SEQ ID NO： 109 
戈氏链球菌(Streptococcus gordonii)tmRNA 
GGGGUCGUUACGGAUUCGACAGGCAUUAUGAGGCAUAUUUUGCGACUCAUCUAGCGGAUGUAAAACGCCAGUUAAAUAUAACUGCAAAAAAUAAUACUUCUUACGCUUUAGCUGCCUAAAAACCAGCGGGCGUGACCCGAUUCGGAUUGCUUGUGUCUGAUGACAGGUCUUAUUAUUAGCAAGCUAC GGUAGAAUCUUGUCUAGUGAUUUUACAAGAGAUUGAUAGACUCGCUUGAUUUGGGCUUGAGUUAUGUGUCAAAAUCAAGUUAAAACAAUACAUAGC CUAUGGUUGUAGACAAAUGUGUUGGCAGAUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA SEQ ID NO：110 
变异链球菌(Streptococcus mutans)ssrA 
GGGGTCGTTACGGATTCGACAGGCATTATGAGACCTATTTTGCGACTCATCTAGCGGATGTAAAACGCCAGTTAAATATAACTGCAAAAAATACAAATTCTTACGCAGTAGCTGCCTAAAAACCAGCCTGTGTGATCAATAACAAATTGCTTGTGTTTGTTGATTGGTCTTATTGTTAACAAGCTACGTTAGAACTGAGTCAGGCTGTTCTAAAAGAGTTCTACTGACTCGCATCGTTAGAGTTTGAGTTATGTATTGTAACGGTGTTAAATAAACACATAACCTATAGTTGTAGACAAATGGGTTAGCAGATGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCASEQ ID NO：111 
变异链球菌(Streptococcus mutans)tmRNA 
GGGGUCGUUACGGAUUCGACAGGCAUUAUGAGACCUAUUUUGCGACUCAUCUAGCGGAUGUAAAACGCCAGUUAAAUAUAACUGCAAAAAAUACAAAUUCUUACGCAGUAGCUGCCUAAAAACCAGCCUGUGUGAUCAAUAACAAAUUGCUUGUGUUUGUUGAUUGGUCUUAUUGUUAACAAGCUACGUUAGAACUGAGUCAGGCUGUUCUAAAA GAGUUCUACUGACUCGCAUCGUUAGAGUUUGAGUUAUGUAUUGUAACGGUGUUAAAUAAACACAUAACCUAUAGUUGUAGACAAAUGGGUUAGCAGAUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA SEQ ID NO：112 
肺炎链球菌(Streptococcus pneumoniae)ssrA 
GGGGTCGTTACGGATTCGACAGGCATTATGAGGCATATTTTGCGACTCGTGTGGCGACGTAAACGCTCAGTTAAATATAACTGCAAAAAATAACACTTCTTACGCTCTAGCTGCCTAAAAACCAGCAGGCGTGACCCGATTTGGATTGCTCGTGTTCAATGACAGGTCTTATTATTAGCGAGATACGATTAAGCCTTGTCTAGCGGTTTGATAAGAGATTGATAGACTCGCAGTTTCTAGACTTGAGTTATGTGTCGAGGGGCTGTTAAAATAATACATAACCTATGGTTGTAGACAAATATGTTGGCAGGTGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCASEQ ID NO：113 
肺炎链球菌(Streptococcus pneumoniae)tmRNA 
GGGGUCGUUACGGAUUCGACAGGCAUUAUGAGGCAUAUUUUGCGACUCGUGUGGCGACGUAAACGCUCAGUUAAAUAUAACUGCAAAAAAUAACACUUCUUACGCUCUAGCUGCCUAAAAACCAGCAGGCGUGACCCGAUUUGGAUUGCUCGUGUUCAAUGACAGGUCUUAUUAUUAGCGAGAUACGAUUAAGCCUUGUCUAGCGGUUUGAUAAGAGAUUGAUAGACUCGCAGUUUCUAGACUUGAGUUAUGUGUCGAGGGGCUGUUAAAAUAAUACAUAACCUAUGGUUGUAGACAAAUAUGUUGGCAGGUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA SEQ ID NO：114 
化脓链球菌(Streptococcus pyogenes)ssrA 
GGGGTTGTTACGGATTCGACAGGCATTATGAGGCATGTTTTGCGTCCCATCGGCAGATGTAAATTGCCAGTTAAATATAACTGCAAAAA ATACAAACTCTTACGCTTTAGCTGCCTAAAAACCAGCTAGCGTGACTTCTACAAGATTGCTTGTGTCCTGTTAGAAGTCTCAAAATAGCAAGCTACGGTTACGAAATTGTCTAGTTTCGTGACAAGAGATTGATAGACTCGCAAACTAATGGCTTGAGTTATGTGTCTTTAGTTTGTTAAATGAAGACATAACCTATGGACGTAGACAAATATGTTGGCAGGTGTTTGGACGTGGGTTCGACTCCCACCAGCTCCACCASEQ ID NO：115 
化脓链球菌(Streptococcus pyogenes)tmRNA 
GGGGUUGUUACGGAUUCGACAGGCAUUAUGAGGCAUGUUUUGCGUCCCAUCGGCAGAUGUAAAUUGCCAGUUAAAUAUAACUGCAAAAAAUACAAACUCUUACGCUUUAGCUGCCUAAAAACCAGCUAGCGUGACUUCUACAAGAUUGCUUGUGUCCUGUUAGAAGUCUCAAAAUAGCAAGCUACGGUUACGAAAUUGUCUAGUUUCGUGACAAGAGAUUGAUAGACUCGCAAACUAAUGGCUUGAGUUAUGUGUCUUUAGUUUGUUAAAUGAAGACAUAACCUAUGGACGUAGACAAAUAUGUUGGCAGGUGUUUGGACGUGGGUUCGACUCCCACCAGCUCCACCA SEQ ID NO：116 
Synechococcus sp.PCC6301ssrA 
GGGGCTGTAATGGTTTCGACGTGTTGGTGAATCCTTCACCGTGATTCAGGCCGAGAGGGAGTCCACTCTCGTAAATCCAGGCTCAACCAAAAGTAACTGCGAACAACATCGTTCCTTTCGCTCGTAAGGCTGCTCCTGTAGCTGCTTAAACGCCACAAACTTTCTGGCTCGAGCGTCTAGTCGTAGACTCCGTTAATACGCCTAGACTTAAACCCCCAACGGATGCTCGAGTGGCGGCCTCAGGTCCGTCCTCTCGCTAAGCAAAAACCTGAGCATCCCGCCAACGGGGATAATCGTTGGCTCCCGCACAGTGGGTCAACCGTGCTAAGCCTGTGAACGAGCGGAAAGTTACTAGTCAATGCGGACAGCGGTTCGATTCCGCTCAGCTCCACCASEQ ID NO：117 
Synechococcus sp.PCC6301tmRNA 
GGGGCUGUAAUGGUUUCGACGUGUUGGUGAAUCCUUCACCGUGAUUCAGGCCGAGAGGGAGUCCACUCUCGUAAAUCCAGGCUCAACCAAAAGUAACUGCGAACAACAUCGUUCCUUUCGCUCGUAAGGCUGCUCCUGUAGCUGCUUAAACGCCACAAACUUUCUGGCUCGAGCGUCUAGUCGUAGACUCCGUUAAUACGCCUAGACUUAAACCCCCAACGGAUGCUCGAGUGGCGGCCUCAGGUCCGUCCUCUCGCUAAGCAAAAACCUGAGCAUCCCGCCAACGGGGAUAAUCGUUGGCUCCCGCACAGUGGGUCAACCGUGCUAAGCCUGUGAACGAGCGGAAAGUUACUAGUCAAUGCGGACAGCGGUUCGAUUCCGCUCAGCUCCACCA SEQ ID NO：118 
Synechocystis sp.PCC6803ssrA 
GGGGCCGCAATGGTTTCGACAGGTTGGCGAAAGCTTGCCCGTGATACAGGTCGAGAGTGAGTCTCCTCTCGCAAATCAAAGGCTCAAAAAAAAGTAACTGCGAATAACATCGTCAGCTTCAAACGGGTAGCCATAGCAGCCTAGTCTGTAAAAGCTACATTTTCTTGTCAAAGACCGTTTACTTCTTTTCTGACTCCGTTAAGGATTAGAGGTTAACCCCAACGGATGCTTTGTTTGGCTCTTCTCTAGTTAGCTAAACAATCAAGACTCAGACTAGAGCATCCCACCATCAGGGATAATCGATGGTCCCCGTCCTAGGGCTAGAAGGACTAAACCTGTGAATGAGCGGAAAGTTAATACCCAGTTTGGACAGCAGTTCAATTCTGCTCGGCTCCACCASEQ ID NO：119 
Synechocystis sp.PCC6803tmRNA 
GGGGCCGCAAUGGUUUCGACAGGUUGGCGAAAGCUUGCCCGUGAUACAGGUCGAGAGUGAGUCUCCUCUCGCAAAUCAAAGGCUCAAAAAAAAGUAACUGCGAAUAACAUCGUCAGCUUCAAACGGGUAGCCAUAGCAGCCUAGUCUGUAAAAGCUACAUUUUCUUGUCAAA GACCGUUUACUUCUUUUCUGACUCCGUUAAGGAUUAGAGGUUAACCCCAACGGAUGCUUUGUUUGGCUCUUCUCUAGUUAGCUAAACAAUCAAGACUCAGACUAGAGCAUCCCACCAUCAGGGAUAAUCGAUGGUCCCCGUCCUAGGGCUAGAAGGACUAAACCUGUGAAUGAGCGGAAAGUUAAUACCCAGUUUGGACAGCAGUUCAAUUCUGCUCGGCUCCACCA SEQ ID NO：120 
海栖热袍菌(Thermotoga maritima)ssrA 
GGGGGCGAACGGGTTCGACGGGGATGGAGTCCCCTGGGAAGCGAGCCGAGGTCCCCACCTCCTCGTAAAAAAGGTGGGACAAAGAATAAGTGCCAACGAACCTGTTGCTGTTGCCGCTTAATAGATAAGCGGCCGTCCTCTCCGAAGTTGGCTGGGCTTCGGAAGAGGGCGTGAGAGATCCAGCCTACCGATTCAGCTTCGCCTTCCGGCCTGAATCGGGAAAACTCAGGAAGGCTGTGGGAGAGGACACCCTGCCCGTGGGAGGTCCCTCCCGAGAGCGAAAACACGGGCTGCGCTCGGAGAAGCCCAGGGGCCTCCATCTTCGGACGGGGGTTCGAATCCCCCCGCCTCCACCA SEQ ID NO：121 
海栖热袍菌(Thermotoga maritima)tmRNA 
GGGGGCGAACGGGUUCGACGGGGAUGGAGUCCCCUGGGAAGCGAGCCGAGGUCCCCACCUCCUCGUAAAAAAGGUGGGACAAAGAAUAAGUGCCAACGAACCUGUUGCUGUUGCCGCUUAAUAGAUAAGCGGCCGUCCUCUCCGAAGUUGGCUGGGCUUCGGAAGAGGGCGUGAGAGAUCCAGCCUACCGAUUCAGCUUCGCCUUCCGGCCUGAAUCGGGAAAACUCAGGAAGGCUGUGGGAGAGGACACCCUGCCCGUGGGAGGUCCCUCCCGAGAGCGAAAACACGGGCUGCGCUCGGAGAAGCCCAGGGGCCUCCAUCUUCGGACGGGGGUUCGAAUCCCCCCGCCUCCACCA SEQ ID NO：122 
嗜热栖热菌(Thermus thermophilus)ssrA GGGGGTGAAACGGTCTCGACGGGGGTCGCCGAGGGCGTGGCTGCGCGCCGAGGTGCGGGTGGCCTCGTAAAAACCCGCAACGGCATAACTGCCAACACCAACTACGCTCTCGCGGCTTAATGACCGCGACCTCGCCCGGTAGCCCTGCCGGGGGCTCACCGGAAGCGGGGACACAAACCCGGCTAGCCCGGGGCCACGCCCTCTAACCCCGGGCGAAGCTTGAAGGGGGCTCGCTCCTGGCCGCCCGTCCGCGGGCCAAGCCAGGAGGACACGCGAAACGCGGACTACGCGCGTAGAGGCCCGCCGTAGAGACCTTCGGACGGGGGTTCGACTCCCCCCACCTCCACCASEQ ID NO：123 
嗜热栖热菌(Thermus thermophilus)tmRNA 
GGGGGUGAAACGGUCUCGACGGGGGUCGCCGAGGGCGUGGCUGCGCGCCGAGGUGCGGGUGGCCUCGUAAAAACCCGCAACGGCAUAACUGCCAACACCAACUACGCUCUCGCGGCUUAAUGACCGCGACCUCGCCCGGUAGCCCUGCCGGGGGCUCACCGGAAGCGGGGACACAAACCCGGCUAGCCCGGGGCCACGCCCUCUAACCCCGGGCGAAGCUUGAAGGGGGCUCGCUCCUGGCCGCCCGUCCGCGGGCCAAGCCAGGAGGACACGCGAAACGCGGACUACGCGCGUAGAGGCCCGCCGUAGAGACCUUCGGACGGGGGUUCGACUCCCCCCACCUCCACCA SEQ ID NO：124 
苍白密螺旋体(Treponema pallidum)ssrA 
GGGGATGACTAGGTTTCGACTAGGGATGTGGGGTGTTGCGCTGCAGGTGGAGTGTCGATCTCCTGATTCGGCGCCTTTATAACTGCCAATTCTGACAGTTTCGACTACGCGCTCGCCGCGTAATCGCGGGCCTGTGTTTGCGCTGCTCTGAGCGAACATATCGGCCCGACGCCAAACGGAGCTTGCTCTTACGTTGTGCACGGCGGACGTAGGGGGACTTTTGTCTGTGCTAAGACTCTGGCGCGTGCGGTGCAGGCCTAGCAGAGTCCGACAAACGCAGTACGCACCGCTAAACCTGTAGGCGCGCAGCACTCGCTCTTTAGGACGGGGGTTCGATTCCCCCCATCTCCACCA SEQ ID NO：125 
苍白密螺旋体(Treponema pallidum)tmRNA 
GGGGAUGACUAGGUUUCGACUAGGGAUGUGGGGUGUUGCGCUGCAGGUGGAGUGUCGAUCUCCUGAUUCGGCGCCUUUAUAACUGCCAAUUCUGACAGUUUCGACUACGCGCUCGCCGCGUAAUCGCGGGCCUGUGUUUGCGCUGCUCUGAGCGAACAUAUCGGCCCGACGCCAAACGGAGCUUGCUCUUACGUUGUGCACGGCGGACGUAGGGGGACUUUUGUCUGUGCUAAGACUCUGGCGCGUGCGGUGCAGGCCUAGCAGAGUCCGACAAACGCAGUACGCACCGCUAAACCUGUAGGCGCGCAGCACUCGCUCUUUAGGACGGGGGUUCGAUUCCCCCCAUCUCCACCA SEQ ID NO：126 
霍乱弧菌(Vibrio cholerae)ssrA 
GGGGCTGATTCAGGATTCGACGGGAATTTTGCAGTCTGAGGTGCATGCCGAGGTGCGGTAGGCCTCGTTAACAAACCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCACTAGCAGCTTAATACCCTGCTCAGAGCCCTTCCTCCCTAGCTTCCGCTTGTAAGACGGGGAAATCAGGAAGGTCAAACCAAATCAAGCTGGCGTGGATTCCCCCACCTGAGGGATGAAGCGCGAGATCTAATTCAGGTTAGCCATTCGTTAGCGTGTCGGTTCGCAGGCGGTGGTGAAATTAAAGATCGACTAAGCATGTAGTACCAAAGATGAATGGTTTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA SEQ ID NO：127 
霍乱弧菌(Vibrio cholerae)tmRNA 
GGGGCUGAUUCAGGAUUCGACGGGAAUUUUGCAGUCUGAGGUGCAUGCCGAGGUGCGGUAGGCCUCGUUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCACUAGCAGCUUAAUACCCUGCUCAGAGCCCUUCCUCCCUAGCUUCCGCUUGUAAGACGGGGAAAUCAGGAAGGUCAAACCAAAUCAAGCUGGCGUGGAUUCCCC CACCUGAGGGAUGAAGCGCGAGAUCUAAUUCAGGUUAGCCAUUCGUUAGCGUGUCGGUUCGCAGGCGGUGGUGAAAUUAAAGAUCGACUAAGCAUGUAGUACCAAAGAUGAAUGGUUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA SEQ ID NO：128 
鼠疫耶尔森氏菌(Yersinia pestis)ssrA 
GGGGCTGATTCTGGATTCGACGGGATTCGCGAAACCCAAGGTGCATGCCGAGGTGCGGTGGCCTCGTAAAAAACCGCAAAAAAAATAGTTGCAAACGACGAAAACTACGCACTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTGCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCTAAAAGAGCTCGTGTGGAAACCTTGCCTGGGGTGGAAGCATTAAAACTAATCAGGATAGTTTGTCAGTAGCGTGTCCATCCGCAGCTGGCCGGCGAATGTAATGATTGGACTAAGCATGTAGTGCCGACGGTGTAGTAATTTCGGACGGGGGTTCAAATCCCCCCAGCTCCACCA SEQ ID NO：129 
鼠疫耶尔森氏菌(Yersinia pestis)tmRNA 
GGGGCUGAUUCUGGAUUCGACGGGAUUCGCGAAACCCAAGGUGCAUGCCGAGGUGCGGUGGCCUCGUAAAAAACCGCAAAAAAAAUAGUUGCAAACGACGAAAACUACGCACUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUGCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCUAAAAGAGCUCGUGUGGAAACCUUGCCUGGGGUGGAAGCAUUAAAACUAAUCAGGAUAGUUUGUCAGUAGCGUGUCCAUCCGCAGCUGGCCGGCGAAUGUAAUGAUUGGACUAAGCAUGUAGUGCCGACGGUGUAGUAAUUUCGGACGGGGGUUCAAAUCCCCCCAGCUCCACCA SEQ ID NO：130 
胎儿弯曲杆菌(Campylobacter fetus)ssrA，内部部分 
AGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGGCAAAGCGTAAAAAGCCCAAATAAAATTAAACGCAAACAACGTTAAATTCGCTC CTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCTGAAATTTAAGTCATACTATCTAGCTTAATTTTCGGTCATCTTTGATAGTGTAGCCTTGCGTTTGACAAGCGTTGAGGTGAAATAAAGTCTTAGCCTTGCTTTTGAGTTTTGGAAGATGAGCGAAGTAGGGTGAAGTAGTCATCTTTGCTAAGCATGTAGAGGTCTTTGTGGGATTATTTTTGGSEQ ID NO：131 
胎儿弯曲杆菌(Campylobacter fetus)tmRNA，内部部分 
AGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGGCAAAGCGUAAAAAGCCCAAAUAAAAUUAAACGCAAACAACGUUAAAUUCGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCUGAAAUUUAAGUCAUACUAUCUAGCUUAAUUUUCGGUCAUCUUUGAUAGUGUAGCCUUGCGUUUGACAAGCGUUGAGGUGAAAUAAAGUCUUAGCCUUGCUUUUGAGUUUUGGAAGAUGAGCGAAGUAGGGUGAAGUAGUCAUCUUUGCUAAGCAUGUAGAGGUCUUUGUGGGAUUAUUUUUGG SEQ ID NO：132 
大肠弯曲杆菌(Campylobacter coli)(BM2509)ssrA，内部部分 
AGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGACAAAGCGTAAAAAGTCCAAATTAAAATTAAACGCAAATAACGTTAAATTTGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCCGAAACTCAAGTGATGCTATCTAGCTTGAATTTTGGTCATCTTTGATAGTGTAGATTGAAAATTGACAACTTTTAATCGAAGTTAAAGTCTTAGTCTAGCTTGAAATTTTGGAAGGTGAGTTTAGCCAGATGAAGTTTTCACCTTTGCTAAACATGTAGAAGTCTTTGTGGGGTTATTTTTGGSEQ ID NO：133 
大肠弯曲杆菌(Campylobacter coli)(BM2509)tmRNA，内部部分 
AGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGACAAAGCGUAAAAAGUCCAAAUUAAAAUUAAACGCAAAUAACGUUAAAUUU GCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCCGAAACUCAAGUGAUGCUAUCUAGCUUGAAUUUUGGUCAUCUUUGAUAGUGUAGAUUGAAAAUUGACAACUUUUAAUCGAAGUUAAAGUCUUAGUCUAGCUUGAAAUUUUGGAAGGUGAGUUUAGCCAGAUGAAGUUUUCACCUUUGCUAAACAUGUAGAAGUCUUUGUGGGGUUAUUUUUGG SEQ ID NO：134 
鸡弯曲杆菌分离物(Camplyobacter chicken isolate)ssrA，内部部分 
ACAGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGGCAAAGCGTAAAAAGCCCAAATAAAATTAAACGCAAACAACGTTAAATTCGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCTGAAATTTAAGTCATACTATCTAGCTTAATTTTCGGTCATTTTTGATAGTGTAGCCTTGCGTTTGACAAGCGTTGAGGTGAAATAAGGTCTTAGCCTTGCTTTTGAGTTTTGGAAGATGAGCGAAGTAGGGTGAAGTAGTCATCTTTGCTAAGCATGTAGAGGTCTTTGTGGGATTATTTTTGGSEQ ID NO：135 
鸡弯曲杆菌分离物(Camplyobacter chicken isolate)tmRNA，内部部分 
ACAGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGGCAAAGCGUAAAAAGCCCAAAUAAAAUUAAACGCAAACAACGUUAAAUUCGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCUGAAAUUUAAGUCAUACUAUCUAGCUUAAUUUUCGGUCAUUUUUGAUAGUGUAGCCUUGCGUUUGACAAGCGUUGAGGUGAAAUAAGGUCUUAGCCUUGCUUUUGAGUUUUGGAAGAUGAGCGAAGUAGGGUGAAGUAGUCAUCUUUGCUAAGCAUGUAGAGGUCUUUGUGGGAUUAUUUUUGG SEQ ID NO：136 
产气荚膜梭菌(Clostridium perfringens)ssrA，内部部分 
ACGGGGGTAGGATGGGTTTGATAAGCGAGTCGAGGGAAGCATGGTGCCTCGATAATAAAGTATGCATTAAAGATAAACGCACGAGATA ATTTTGCATTAGCAGCTTAAGTTAGCGCTGCTCATCCTTCCTCAATTGCCCACGGTTGAGAGTAAGGGTGTCATTTAAAAGTGGGGAACCGAGCCTAGCAAAGCTTTGAGCTAGGAACGGAATTTATGAAGCTTACCAAAGAGGAAGTTTGTCTGTGGACGTTCTCTGAGGGAATTTTAAAACACAAGACTACACTCGTAGAAAGTCTTACTGGTCTGCTTTCGG SEQ ID NO：137 
产气荚膜梭菌(Clostridium perfringens)tmRNA，内部部分 
ACGGGGGUAGGAUGGGUUUGAUAAGCGAGUCGAGGGAAGCAUGGUGCCUCGAUAAUAAAGUAUGCAUUAAAGAUAAACGCACGAGAUAAUUUUGCAUUAGCAGCUUAAGUUAGCGCUGCUCAUCCUUCCUCAAUUGCCCACGGUUGAGAGUAAGGGUGUCAUUUAAAAGUGGGGAACCGAGCCUAGCAAAGCUUUGAGCUAGGAACGGAAUUUAUGAAGCUUACCAAAGAGGAAGUUUGUCUGUGGACGUUCUCUGAGGGAAUUUUAAAACACAAGACUACACUCGUAGAAAGUCUUACUGGUCUGCUUUCGG SEQ ID NO：138 
杜氏嗜血菌(Haemophilus ducreyi)(NCTC 10945)ssrA，内部部分 
ACGGGATTAGCGAAGTCCAAGGTGCACGTCGAGGTGCGGTAGGCCTCGTAACAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCATTTAGCCTTCGCGCCCTAGCTTTCGCTCGTAAGACGGGGAGCACGCGGAGTCAAACCAAAACGAGATCGTGTGGACGCTTCCGCTTGTAGATGAAACACTAAATTGAATCAAGCTAGTTTATTTCTTGCGTGTCTGTCCGCTGGAGATAAGCGAAATTAAAGACCAGACTAAACGTGTAGTACTGAAGATAGAGTAATTTCGGACCCGGGTTCGACTC SEQ ID NO：139 
杜氏嗜血菌(Haemophilus ducreyi)(NCTC 10945)tmRNA，内部部分 
ACGGGAUUAGCGAAGUCCAAGGUGCACGUCGAGGUGCGGUAGGCCUCGUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAACAA UACGCUUUAGCAGCUUAAUAACCUGCAUUUAGCCUUCGCGCCCUAGCUUUCGCUCGUAAGACGGGGAGCACGCGGAGUCAAACCAAAACGAGAUCGUGUGGACGCUUCCGCUUGUAGAUGAAACACUAAAUUGAAUCAAGCUAGUUUAUUUCUUGCGUGUCUGUCCGCUGGAGAUAAGCGAAAUUAAAGACCAGACUAAACGUGUAGUACUGAAGAUAGAGUAAUUUCGGACCCGGGUUCGACUCSEQ ID NO：140 
无害李斯特氏菌(Listeria innocua)(食物分离物#1)ssrA，内部部分 
GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAACCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAA SEQ ID NO：141 
无害李斯特氏菌(Listeria innocua)(食物分离物#1)tmRNA，内部部分 
GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAACCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAA SEQ ID NO：142 
无害李斯特氏菌(Listeria innocua)(食物分离物#2)ssrA，内部部分 
GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTA GATATTTAA SEQ ID NO：143 
无害李斯特氏菌(Listeria innocua)(食物分离物#2)tmRNA，内部部分 
GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAA SEQ ID NO：144 
无害李斯特氏菌(Listeria innocua)(食物分离物#3)ssrA，内部部分 
GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGAATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCGGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAA SEQ ID NO：145 
无害李斯特氏菌(Listeria innocua)(食物分离物#3)tmRNA，内部部分 
GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGAAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCGGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAA SEQ ID NO：146 
无害李斯特氏菌(Listeria innocua)(ATCC 12210)ssrA，内部部分 
GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTA AATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACTGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAA SEQ ID NO：147 
无害李斯特氏菌(Listeria innocua)(ATCC 12210)tmRNA，内部部分 
GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACUGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAA SEQ ID NO：148 
伊氏李斯特氏菌(Listeria ivanovii)(NCTC 11846)ssrA，内部部分 
ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATTAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG SEQ ID NO：149 
伊氏李斯特氏菌(Listeria ivanovii)(NCTC 11846)tmRNA，内部部分 
ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUUAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUCGCUCGUAGAUAU UUAAGUGCCGAUAUUUCUGG SEQ ID NO：150 
斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)ssrA，内部部分 
ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGAAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATACTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCCATATTTCTGG SEQ ID NO：151 
斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)tmRNA，内部部分 
ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGAAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUACUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCCAUAUUUCUGG SEQ ID NO：152 
肠炎沙门氏菌(Salmonella enteritidis)ssrA，内部部分 
ACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAACCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGATGCCCTGCCTGGGGTTGAAGCGTTAAAACGAATCAGGCTAGTCTGGTAGTGGCGTGTCCGTCCGCAGGTGCCAGGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAAT TTCGG SEQ ID NO：153 
肠炎沙门氏菌(Salmonella enteritidis)tmRNA，内部部分 
ACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAACCUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAUGCCCUGCCUGGGGUUGAAGCGUUAAAACGAAUCAGGCUAGUCUGGUAGUGGCGUGUCCGUCCGCAGGUGCCAGGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGG SEQ ID NO：154 
表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)ssrA，内部部分 
ACAGGGGTCCCCCGAGCTTATTAAGCGTGTCGGAGGGTTGGCTCCGTCATCAACACATTTCGGTTAAATATAACTGACAAATCAAACAATAATTTCGCAGTAGCTGCGTAATAGCCACTGCATCGCCTAACAGCATCTCCTACGTGCTGTTAACGCGATTCAACCCTAGTAGGATATGCTAAACACTGCCGCTTGAAGTCTGTTTAGATGAAATATAATCAAGCTAGTATCATGTTGGTTGTTTATTGCTTAGCATGATGCGAAAATTATCAATAAACTACACACGTAGAAAGATTTGTATCAGGACCTCTGGSEQ ID NO：155 
表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)tmRNA，内部部分 
ACAGGGGUCCCCCGAGCUUAUUAAGCGUGUCGGAGGGUUGGCUCCGUCAUCAACACAUUUCGGUUAAAUAUAACUGACAAAUCAAACAAUAAUUUCGCAGUAGCUGCGUAAUAGCCACUGCAUCGCCUAACAGCAUCUCCUACGUGCUGUUAACGCGAUUCAACCCUAGUAGGAUAUGCUAAACACUGCCGCUUGAAGUCUGUUUAGAUGAAAUA UAAUCAAGCUAGUAUCAUGUUGGUUGUUUAUUGCUUAGCAUGAUGCGAAAAUUAUCAAUAAACUACACACGUAGAAAGAUUUGUAUCAGGACCUCUGG SEQ ID NO：156 
无乳链球菌(Streptococcus agalactiae)(NCTC 8181)ssrA，内部部分 
ACAGGCATTATGAGGTATATTTTGCGACTCATCGGCAGATGTAAAATGCCAGTTAAATATAACTGCAAAAAATACAAATTCTTACGCATTAGCTGCCTAAAAAACAGCCTGCGTGATCTTCACAAGATTGTTTGCGTTTTGCTAGAAGGTCTTATTTATCAGCAAACTACGTTTGGCTACTGTCTAGTTAGTTAAAAAGAGATTTATAGACTCGCTATGTGAGGGCTTGAGTTATGTGTCATCACCTAGTTAAATCAATACATAACCTATAGTTGTAGACAAATATATTAGCAGATGTTTGGSEQ ID NO：157 
无乳链球菌(Streptococcus agalactiae)(NCTC 8181)tmRNA，内部部分 
ACAGGCAUUAUGAGGUAUAUUUUGCGACUCAUCGGCAGAUGUAAAAUGCCAGUUAAAUAUAACUGCAAAAAAUACAAAUUCUUACGCAUUAGCUGCCUAAAAAACAGCCUGCGUGAUCUUCACAAGAUUGUUUGCGUUUUGCUAGAAGGUCUUAUUUAUCAGCAAACUACGUUUGGCUACUGUCUAGUUAGUUAAAAAGAGAUUUAUAGACUCGCUAUGUGAGGGCUUGAGUUAUGUGUCAUCACCUAGUUAAAUCAAUACAUAACCUAUAGUUGUAGACAAAUAUAUUAGCAGAUGUUUGG SEQ ID NO：158 
支气管炎博德特氏菌(Bordetella bronchiseptica)ssrA 
GGGGCCGATCCGGATTCGACGTGGGTCATGAAACAGCTCAAGGCATGCCGAGCACCAGTAAGCTCGTTAATCCACTGGAACACTACAAACGCCAACGACGAGCGTTTCGCTCTCGCCGCTTAAGCGGTGAGCCGCTGCACTGATCTGTCCTTGGGTCACGCGGGGGAASEQ ID NO：159 
支气管炎博德特氏菌(Bordetella bronchiseptica)tmRNA
GGGGCCGAUCCGGAUUCGACGUGGGUCAUGAAACAGCUCAAGGCAUGCCGAGCACCAGUAAGCUCGUUAAUCCACUGGAACACUACAAACGCCAACGACGAGCGUUUCGCUCUCGCCGCUUAAGCGGUGAGCCGCUGCACUGAUCUGUCCUUGGGUCACGCGGGGGAASEQ ID NO：160 
肺炎衣原体(Chlamydia pneumoniae)(CWL029)，ssrA 
GGGGGTGTATAGGTTTCGACTTGAAAATGAAGTGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCAACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATTAGCTTGTTTGACTCAGTAGAGGAAAGACTAGCTGCTTAATTAGCAAAAGTTGTTAGCTAGATAATCTCTAGGTAACCCGGTATCTGCGAGCTCCACCAGAGGCTTGCAAAATACCGTCATTTATCTGGTTGGAACTTACTTTCTCTAATTCTCAAGGAAGTTCGTTCGAGATTTTTGAGAGTCATTGGCTGCTATAGAGGCTTCTAGCTAAGGGAGTCCAATGTAAACAATTCTAGAAGATAAGCATGTAGAGGTTAGCAGGGAGTTTGTCAAGGACGAGAGTTCGAGTCTCTCCACCTCCACCA SEQ ID NO：161 
肺炎衣原体(Chlamydia pneumoniae)(CWL029)tmRNA 
GGGGGUGUAUAGGUUUCGACUUGAAAAUGAAGUGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCAACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUUAGCUUGUUUGACUCAGUAGAGGAAAGACUAGCUGCUUAAUUAGCAAAAGUUGUUAGCUAGAUAAUCUCUAGGUAACCCGGUAUCUGCGAGCUCCACCAGAGGCUUGCAAAAUACCGUCAUUUAUCUGGUUGGAACUUACUUUCUCUAAUUCUCAAGGAAGUUCGUUCGAGAUUUUUGAGAGUCAUUGGCUGCUAUAGAGGCUUCUAGCUAAGGGAGUCCAAUGUAAACAAUUCUAGAAGAUAAGCAUGUAGAGGUUAGCAGGGAG UUUGUCAAGGACGAGAGUUCGAGUCUCUCCACCUCCACCASEQ ID NO：162 
土拉热弗朗西丝氏菌(Francisella tularensis)ssrA 
GGGGGCGAATATGGTTTCGACATGAATGTCAAAATCTAAGGTGCATGCCGAGGAAGTACCGTAACCTCGTTAATAACAGTACAAATGCCAATAATAACTGGCAACAAAAAAGCAAACCGCGTAGCGGCTAACGACAGCAACTTTGCTGCTGTTGCTAAAGCTGCCTAGTCTAGCTTAATAATCTAGATGCGCACGGATATGATAGTCTTTCTTATGACACTATCTATACATCCGTTCATATTCCGCATAAGACGGTCTTTGCTTTTTGTCTGGGAGTTAAGGCTGTATTTAACAGACTCGCTAACTATTACCCTGGCTAATTGGGGAATAGTCAAGCTAAACTCAAATAGATTAGCCTAAGCATGTAGATCCAAAGATCTAGAGTTTGTGGACGCGGGTTCAAATCCCGCCGCCTCCACCA SEQ ID NO：163 
土拉热弗朗西丝氏菌(Francisella tularensis)tmRNA 
GGGGGCGAAUAUGGUUUCGACAUGAAUGUCAAAAUCUAAGGUGCAUGCCGAGGAAGUACCGUAACCUCGUUAAUAACAGUACAAAUGCCAAUAAUAACUGGCAACAAAAAAGCAAACCGCGUAGCGGCUAACGACAGCAACUUUGCUGCUGUUGCUAAAGCUGCCUAGUCUAGCUUAAUAAUCUAGAUGCGCACGGAUAUGAUAGUCUUUCUUAUGACACUAUCUAUACAUCCGUUCAUAUUCCGCAUAAGACGGUCUUUGCUUUUUGUCUGGGAGUUAAGGCUGUAUUUAACAGACUCGCUAACUAUUACCCUGGCUAAUUGGGGAAUAGUCAAGCUAAACUCAAAUAGAUUAGCCUAAGCAUGUAGAUCCAAAGAUCUAGAGUUUGUGGACGCGGGUUCAAAUCCCGCCGCCUCCACCASEQ ID NO：164 
Guillardia theta(质体)ssrA 
GGGGCTGATTTGGATTCGACATATAAATTTGCGTGTTTCATTATG AAGCAAGTCAAGTTTAATGATCTTGTAAAAAACATTAAAGTACAAATAAATGCAAGCAATATAGTTTCATTTAGTTCAAAACGTTTAGTCTCTTTTGCATAAGCAAAATGTGTTAATAACTTTCTTAGTAGAAATTGGAGAAGTTTACTAAGATTTATATTTACTCCATAATTATTTTAAAGATGGTAAAAAGGTGATTCATCATTTGTATGTTTCTAAACTTTGTGAAAGAATAGTGGGCTCCATTTATAATGAACGTGGGTTCAAATCCCACCAGCTCCACCA SEQ ID NO：165 
Guillardia theta(质体)tmRNA 
GGGGCUGAUUUGGAUUCGACAUAUAAAUUUGCGUGUUUCAUUAUGAAGCAAGUCAAGUUUAAUGAUCUUGUAAAAAACAUUAAAGUACAAAUAAAUGCAAGCAAUAUAGUUUCAUUUAGUUCAAAACGUUUAGUCUCUUUUGCAUAAGCAAAAUGUGUUAAUAACUUUCUUAGUAGAAAUUGGAGAAGUUUACUAAGAUUUAUAUUUACUCCAUAAUUAUUUUAAAGAUGGUAAAAAGGUGAUUCAUCAUUUGUAUGUUUCUAAACUUUGUGAAAGAAUAGUGGGCUCCAUUUAUAAUGAACGUGGGUUCAAAUCCCACCAGCUCCACCASEQ ID NO：166 
Thalassiosira Weissflogii(质体)ssrA 
GGGGCTGATTTGGTTTCGACATTTAAAACTTCTTTCTATGTGTCAGGTCAAAGTTTGTATTCTTTGTAAAAAAATACTAAAATACTAATAAATGCTAATAATATAATACCGTTTATTTTTAAAGCAGTAAAAACAAAAAAAGAAGCAATGGCTTTAAATTTTGCTGTATAGTTCATTAACTTAGGTTATTAAATATTTTTTCATTATAACTGGACTTTTCTCTAGTTTATAGTTTAGAATAAATTTAAATTTTGCAAAACTCGTTCGAAAATTTTCGGGCTAAACCTGTAAACGCAAATACTAAGAAATTTTAGATGGACATGGGTTCAATTCCCATCAGTTCCACCASEQ ID NO：167 
Thalassiosira Weissflogii(质体)tmRNA 
GGGGCUGAUUUGGUUUCGACAUUUAAAACUUCUUUCUAUGUGUCAGGUCAAAGUUUGUAUUCUUUGUAAAAAAAUACUAAAAUACUAAUAAAUGCUAAUAAUAUAAUACCGUUUAUUUUUAAAGCAGUAAAAACAAAAAAAGAAGCAAUGGCUUUAAAUUUUGCUGUAUAGUUCAUUAACUUAGGUUAUUAAAUAUUUUUUCAUUAUAACUGGACUUUUCUCUAGUUUAUAGUUUAGAAUAAAUUUAAAUUUUGCAAAACUCGUUCGAAAAUUUUCGGGCUAAACCUGUAAACGCAAAUACUAAGAAAUUUUAGAUGGACAUGGGUUCAAUUCCCAUCAGUUCCACCA SEQ ID NO：168 
幽门螺杆菌(Helicobacter pylori)ssrA，(临床分离物1)，内部部分 
TGGGGATGTTACGGTTTCGACAGGGGTAGTTCGAGCTTAGGTGGCGAGTCGAGGGGATCGGCCTCGTTAAAACGTCAAAGCCTATAACTGGCAAACAACAAAACAACTTCGCTTTAGCAGCTTAATAAGCTCTTAGCGGTTCCTCCCTCCATCGCCCATGTGGTAGGGTAAGGGACTCAAATTAAGTGGGCTACGCTGGATTCCACCGTCTGAGGATGAAAGAAGAGAACAACCAGACTAGCTACCCGGACGCCCGTCGATAGGCAGATGGAGTAGCGAATCGCGAATATATCGACTACACTCGTAGAAGCTTAAGTGCCGATATTCTTGGACGTGGGTTCGACTCCCSEQ ID NO：176 
幽门螺杆菌(Helicobacter pylori)tmRNA，(临床分离物1)，内部部分 
UGGGGAUGUUACGGUUUCGACAGGGGUAGUUCGAGCUUAGGUGGCGAGUCGAGGGGAUCGGCCUCGUUAAAACGUCAAAGCCUAUAACUGGCAAACAACAAAACAACUUCGCUUUAGCAGCUUAAUAAGCUCUUAGCGGUUCCUCCCUCCAUCGCCCAUGUGGUAGGGUAAGGGACUCAAAUUAAGUGGGCUACGCUGGAUUCCACCGUCUGAGGAUGAAAGAAGAGAACAACCAGACUAGCUACCCGGACGCCCGUCGAUAGGCAGAUGGAGUAGCGAAUCGCGAAUAUAUCGACUACAC UCGUAGAAGCUUAAGUGCCGAUAUUCUUGGACGUGGGUUCGACUCCC SEQ ID NO：177 
幽门螺杆菌(Helicobacter pylori)ssrA，(临床分离物2)，内部部分 
TGGGGACGTTACGGTTTCGACAGGGATAGTTCGAGCTTAGGTTGCGAGTCGAGGGGATCGGCCTCGTTAAAACGTCAAAGCCTATAATTGGCAAACAAAACAATCTTTCTTTAGCTGCTTAATTGCACTAAAGGTTCCTCCCTCCATCGTCCATGTGGTAGGGTAAGGGACTCAAACTAAGTGGACTACGCCGGAGTTCGCCGTCTGAGGACAAAGGAAGAGAACAACCAGACTAGCAACTTGGAAGCCTGTCGATAGGCCGAAGAGTTCGCGAAATGCTAATATATCGACTACACTCGTAGAAGCTTAAGTGCCGATATTTTTGGACGTGGGTTCGATTCCCT SEQ ID NO：178 
幽门螺杆菌(Helicobacter pylori)tmRNA，(临床分离物2)，内部部分 
UGGGGACGUUACGGUUUCGACAGGGAUAGUUCGAGCUUAGGUUGCGAGUCGAGGGGAUCGGCCUCGUUAAAACGUCAAAGCCUAUAAUUGGCAAACAAAACAAUCUUUCUUUAGCUGCUUAAUUGCACUAAAGGUUCCUCCCUCCAUCGUCCAUGUGGUAGGGUAAGGGACUCAAACUAAGUGGACUACGCCGGAGUUCGCCGUCUGAGGACAAAGGAAGAGAACAACCAGACUAGCAACUUGGAAGCCUGUCGAUAGGCCGAAGAGUUCGCGAAAUGCUAAUAUAUCGACUACACUCGUAGAAGCUUAAGUGCCGAUAUUUUUGGACGUGGGUUCGAUUCCCUSEQ ID NO：179 
斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)ssrA，内部部分 
ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGAAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAA TCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATACTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCCATATTTCTGG SEQ ID NO：180 
斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)tmRNA，内部部分 
ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGAAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUACUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCCAUAUUUCUGG SEQ ID NO：181 
伊氏李斯特氏菌(Listeria ivanovii)(NCTC 11846)ssrA，内部部分 
ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATTAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG SEQ ID NO：182 
伊氏李斯特氏菌(Listeria ivanovii)(NCTC 11846)tmRNA，内部部分 
ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUUAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAA GAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUCGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG SEQ ID NO：183 
非洲分枝杆菌(Mycobacterium africanum)(临床分离物)ssrA，内部部分 
ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA SEQ ID NO：184 
非洲分枝杆菌(Mycobacterium africanum)(临床分离物)tmRNA，内部部分 
ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA SEQ ID NO：185 
戈登氏分枝杆菌(Mycobacterium gordonae)(临床分离物)ssrA，内部部分 
ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCATATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCGGACCGGGAACGCCCTCGCCCCGGACCCCGGCATCAGCTAGAGGG ATCAACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTCCGTGTGACCAGGAGATCCGAGCAGAGACATAGCGGACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA SEQ ID NO：186 
戈登氏分枝杆菌(Mycobacterium gordonae)(临床分离物)tmRNA，内部部分 
ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAUAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCGGACCGGGAACGCCCUCGCCCCGGACCCCGGCAUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUCCGUGUGACCAGGAGAUCCGAGCAGAGACAUAGCGGACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA SEQ ID NO：187 
堪萨斯分枝杆菌(Mycobacterium kansasii)(临床分离物)ssrA，内部部分 
ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGACCGCCCTCGACCCGGACTCTGGCATCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGACTCGTCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGACCAGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA SEQ ID NO：188 
堪萨斯分枝杆菌(Mycobacterium kansasii)(临床分离物)tmRNA，内部部分 
ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUC UGUCAGACCGGGACCGCCCUCGACCCGGACUCUGGCAUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGACUCGUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGACCAGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA SEQ ID NO：189 
龟分枝杆菌(Mycobacterium chelonae)ssrA，内部部分 
ACAGCGAGTCTCGACTTAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCATTGCAACCAATTAAGCGCCGATTCTCATCAGCGCGACTACGCACTCGCTGCCTAAGCGACTGCGTGTCTGTCAGACCGGGAGCGCCCTCAGCCCGGACCCTGGCATCAGCTAGAGGGACAAACTACGGGTTCGGTCGCGGGACCCGTAGGGACATCAAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGCGGGACCGAGAGATCCAAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTAATGAACGGCCGTTG SEQ ID NO：190 
龟分枝杆菌(Mycobacterium chelonae)tmRNA，内部部分 
ACAGCGAGUCUCGACUUAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCAUUGCAACCAAUUAAGCGCCGAUUCUCAUCAGCGCGACUACGCACUCGCUGCCUAAGCGACUGCGUGUCUGUCAGACCGGGAGCGCCCUCAGCCCGGACCCUGGCAUCAGCUAGAGGGACAAACUACGGGUUCGGUCGCGGGACCCGUAGGGACAUCAAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGCGGGACCGAGAGAUCCAAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUAAUGAACGGCCGUUG SEQ ID NO：191 
斯氏分枝杆菌(苏加分枝杆菌)(Mycobacterium szulgai)(ATCC 35799)ssrA，内部部分 
ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGAGAACAC TCAGCGCGACTTCGCTCTCGCTGCCTAAGCGACAGCAAGTCCGTCAGACCGGGAAAGCCCTCGACCCGGACCCTGGCGTCATCTAGAGGGATCCACCGGTGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGACCAGGAGATCCGAGTAGAGACATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAG SEQ ID NO：192 
斯氏分枝杆菌(苏加分枝杆菌)(Mycobacterium szulgai)(ATCC 35799)tmRNA，内部部分 
ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAGAACACUCAGCGCGACUUCGCUCUCGCUGCCUAAGCGACAGCAAGUCCGUCAGACCGGGAAAGCCCUCGACCCGGACCCUGGCGUCAUCUAGAGGGAUCCACCGGUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGACCAGGAGAUCCGAGUAGAGACAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAG SEQ ID NO：193 
玛尔摩分枝杆菌(Mycobacterium malmoense)(临床分离物)ssrA，内部部分 
ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCATATAAGCGCCGTTTCAACACAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGACCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTGGAGGGATCCACCGGTGAGTCCGGTCGCGGGACTCATCGGGACATACACAGCGACTGGGATCGTCATCCTGGCTGGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAG SEQ ID NO：194 
玛尔摩分枝杆菌(Mycobacterium malmoense)(临床分离物)tmRNA，内 部部分 
ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAUAUAAGCGCCGUUUCAACACAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGACCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUGGAGGGAUCCACCGGUGAGUCCGGUCGCGGGACUCAUCGGGACAUACACAGCGACUGGGAUCGUCAUCCUGGCUGGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAG SEQ ID NO：195 
微黄分枝杆菌(Mycobacterium flavescens)ssrA，内部部分 
ACTTCGAGCGTCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGATTCCAATCAGCGCGACTACGCACTCGCTGCCTAAGCGACTGCGTGTCTGTCAGCCCGGGAGAGCCCTCGACCCGGTGTCTGGCATCAGCTAGAGGGATAAACCGGTGGGTCCGGTCGCGGGACTCATCGGGACATCAAACAGCGACTGGGATCGTCATCCTGACTTGTTCGCGTGATCAGGAGATCCGAGTAGAGACATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAACGCCGTAG SEQ ID NO：196 
微黄分枝杆菌(Mycobacterium flavescens)tmRNA，内部部分 
ACUUCGAGCGUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAUUCCAAUCAGCGCGACUACGCACUCGCUGCCUAAGCGACUGCGUGUCUGUCAGCCCGGGAGAGCCCUCGACCCGGUGUCUGGCAUCAGCUAGAGGGAUAAACCGGUGGGUCCGGUCGCGGGACUCAUCGGGACAUCAAACAGCGACUGGGAUCGUCAUCCUGACUUGUUCGCGUGAUCAGGAGAUCCGAGUAGAGACAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAACGCCGUAG SEQ ID NO：197 
海分枝杆菌(Mycobacterium marinum)ssrA，内部部分 
ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGATGCAACTAGATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCGGACCGGGAACGCCCTCGCCCCGGACCCCGGCATCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGGCTCATCGGGACATCAACAGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGACCAGGAGATCCGAGCAGAGACCTAGCGGACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAG SEQ ID NO：198 
海分枝杆菌(Mycobacterium marinum)tmRNA，内部部分 
ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGAUGCAACUAGAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCGGACCGGGAACGCCCUCGCCCCGGACCCCGGCAUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGGCUCAUCGGGACAUCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGACCAGGAGAUCCGAGCAGAGACCUAGCGGACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAG SEQ ID NO：199 
田鼠分枝杆菌(Mycobacterium microti)(环境分离物)ssrA，内部部分 
ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAGCCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA SEQ ID NO：200 
田鼠分枝杆菌(Mycobacterium microti)(环境分离物)tmRNA，内部部分 
ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAGCCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA SEQ ID NO：201 
耻垢分枝杆菌(Mycobacterium smegmatis)(ATCC 10143)ssrA，内部部分 
ACTTCGAGCATCGAATCCAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGATTCCAATCAGCGCGACTACGCCCTCGCTGCCTAAGCGACGGCTGGTCTGTCAGACCGGGAGTGCCCTCGGCCCGGATCCTGGCATCAGCTAGAGGGACCCACCCACGGGTTCGGTCGCGGGACCTGTGGGGACATCAAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGTAGAGACATAGCGAACTGCGCACGGAGAAGCCTCGAGGACATGCCGTAG SEQ ID NO：202 
耻垢分枝杆菌(Mycobacterium smegmatis)(ATCC 10143)ssrA，内部部分 
ACUUCGAGCAUCGAAUCCAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAUUCCAAUCAGCGCGACUACGCCCUCGCUGCCUAAGCGACGGCUGGUCUGUCAGACCGGGAGUGCCCUCGGCCCGGAUCCUGGCAUCAGCUAGAGGGACCCACCCACGGGUUCGGUCGCGGGACCUGUGGGGACAUCAAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGUGUGACCGGGAGAUCCGAGUAGAGACAUAGCGAACUGCGCACGGAGAA GCCUCGAGGACAUGCCGUAG SEQ ID NO：203 
蟾(蜍)分枝杆菌(Mycobacterium xenopi)(临床分离物)ssrA，内部部分 
ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACTAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGGCCGGGAGTTCCCTCGACCCGGATCCTGGCGTCAGCTAGAGGGATCCACCGATGGGTTCGGTCGCGGGACCCATCGGGACACCACACAGCGACTGGGATCGCCGTCCCGGCTAGTTCGCGAGACCGGGAGATCCGAGTAAGGGCAAAGCGAACTGCGCACGGAGAAGCCTTGAGGGTATGCCGTA SEQ ID NO：204 
蟾(蜍)分枝杆菌(Mycobacterium xenopi)(临床分离物)tmRNA，内部部分 
ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACUAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGGCCGGGAGUUCCCUCGACCCGGAUCCUGGCGUCAGCUAGAGGGAUCCACCGAUGGGUUCGGUCGCGGGACCCAUCGGGACACCACACAGCGACUGGGAUCGCCGUCCCGGCUAGUUCGCGAGACCGGGAGAUCCGAGUAAGGGCAAAGCGAACUGCGCACGGAGAAGCCUUGAGGGUAUGCCGUA SEQ ID NO：205 
胞内分枝杆菌(Mycobacterium intracellulare)(NCTC 10425)ssrA，内部部分 
ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACCGACCACCGTAAGCGTCGTTGCAAACAGATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGACCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTTATCGGGACACCAAC AGCGACTGGGATCGTCATCTCGGCTTGTTCGCGTGACCGGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGTCTTGAGGGAATGCCGTAG SEQ ID NO：206 
胞内分枝杆菌(Mycobacterium intracellulare)(NCTC 10425)tmRNA，内部部分 
ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACCGACCACCGUAAGCGUCGUUGCAAACAGAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGACCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUUAUCGGGACACCAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGCGUGACCGGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGUCUUGAGGGAAUGCCGUAG SEQ ID NO：207 
淋巴腺炎分枝杆菌(瘰疬分枝杆菌)(Mycobacterium scrofulaceum)(NCTC 10803)ssrA，内部部分 
ACATCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGACCGGGAAAGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTCCGCGTGACCAGGAGATCCGAGCAGAGGCATAGCGGACTGCGCACGGAGAAGTCTTGAGGGAATGCCGTTG SEQ ID NO：208 
淋巴腺炎分枝杆菌(瘰疬分枝杆菌)(Mycobacterium scrofulaceum)(NCTC 10803)tmRNA，内部部分 
ACAUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAUUCA CAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGACCGGGAAAGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUCCGCGUGACCAGGAGAUCCGAGCAGAGGCAUAGCGGACUGCGCACGGAGAAGUCUUGAGGGAAUGCCGUUG SEQ ID NO：209 
星状诺卡氏菌(Nocardia asteroides)ssrA，内部部分 
ACTGTGTGCGCCGAGGTAGGGGAAGCGTGTCGGTGCAGGCTGGAGACCACCGTTAAGCGTCGCGGCAACCAATTAAGCGCCGATTCCAATCAGCGCGACTACGCCCTCGCTGCCTGATCAGCGACGGCTAGCTGTCGGCCCGGGTTGTGTTCCCGAACCCGGATGCCGGCATCATCTCAGGGAACTCACCGTGTTCGCCGGTCGCGGACGGACACGGGACAGCAAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGCGTGACCGGGAGATCCAAGTAGAGACATAGCGGACTGCACACGGAGAAGCCCTACTGACTCGACACAG SEQ ID NO：210 
星状诺卡氏菌(Nocardia asteroides)tmRNA，内部部分 
ACUGUGUGCGCCGAGGUAGGGGAAGCGUGUCGGUGCAGGCUGGAGACCACCGUUAAGCGUCGCGGCAACCAAUUAAGCGCCGAUUCCAAUCAGCGCGACUACGCCCUCGCUGCCUGAUCAGCGACGGCUAGCUGUCGGCCCGGGUUGUGUUCCCGAACCCGGAUGCCGGCAUCAUCUCAGGGAACUCACCGUGUUCGCCGGUCGCGGACGGACACGGGACAGCAAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGCGUGACCGGGAGAUCCAAGUAGAGACAUAGCGGCUGCACACGGAGAAGCCCUACUGACUCGACACAG SEQ ID NO：211 
肠炎沙门氏菌(Salmonella enteritidis)ssrA，内部部分 
ACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAACCTA CGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGATGCCCTGCCTGGGGTTGAAGCGTTAAAACGAATCAGGCTAGTCTGGTAGTGGCGTGTCCGTCCGCAGGTGCCAGGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGG SEQ ID NO：212 
肠炎沙门氏菌(Salmonella enteritidis)tmRNA，内部部分 
ACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAACCUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAUGCCCUGCCUGGGGUUGAAGCGUUAAAACGAAUCAGGCUAGUCUGGUAGUGGCGUGUCCGUCCGCAGGUGCCAGGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGG SEQ ID No：213 
表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)ssrA，内部部分 
ACAGGGGTCCCCCGAGCTTATTAAGCGTGTCGGAGGGTTGGCTCCGTCATCAACACATTTCGGTTAAATATAACTGACAAATCAAACAATAATTTCGCAGTAGCTGCGTAATAGCCACTGCATCGCCTAACAGCATCTCCTACGTGCTGTTAACGCGATTCAACCCTAGTAGGATATGCTAAACACTGCCGCTTGAAGTCTGTTTAGATGAAATATAATCAAGCTAGTATCATGTTGGTTGTTTATTGCTTAGCATGATGCGAAAATTATCAATAAACTACACACGTAGAAAGATTTGTATCAGGACCTCTGGSEQ ID NO：214 
表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)tmRNA，内部部分 
ACAGGGGUCCCCCGAGCUUAUUAAGCGUGUCGGAGGGUUGGCUCCGUCAUCAACACAUUUCGGUUAAAUAUAACUGACAAAUCAAACAAUAAUUUCGCAGUAGCUGCGUAAUAGCCACUGCAUCGCCUAACAGCAUCUCCUACGUGCUGUUAACGCGAUUCAACCCUAGUAGGAUAUGCUAAACACUGCCGCUUGAAGUCUGUUUAGAUGAAAUAUAAUCAAGCUAGUAUCAUGUUGGUUGUUUAUUGCUUAGCAUGAUGCGAAAAUUAUCAAUAAACUACACACGUAGAAAGAUUUGUAUCAGGACCUCUGG SEQ ID NO：215 
无乳链球菌(Streptococcus agalactiae)(NCTC 8181)ssrA，内部部分 
ACAGGCATTATGAGGTATATTTTGCGACTCATCGGCAGATGTAAAATGCCAGTTAAATATAACTGCAAAAAATACAAATTCTTACGCATTAGCTGCCTAAAAAACAGCCTGCGTGATCTTCACAAGATTGTTTGCGTTTTGCTAGAAGGTCTTATTTATCAGCAAACTACGTTTGGCTACTGTCTAGTTAGTTAAAAAGAGATTTATAGACTCGCTATGTGAGGGCTTGAGTTATGTGTCATCACCTAGTTAAATCAATACATAACCTATAGTTGTAGACAAATATATTAGCAGATGTTTGG SEQ ID NO：216 
无乳链球菌(Streptococcus agalactiae)(NCTC 8181)tmRNA，内部部分 
ACAGGCAUUAUGAGGUAUAUUUUGCGACUCAUCGGCAGAUGUAAAAUGCCAGUUAAAUAUAACUGCAAAAAAUACAAAUUCUUACGCAUUAGCUGCCUAAAAAACAGCCUGCGUGAUCUUCACAAGAUUGUUUGCGUUUUGCUAGAAGGUCUUAUUUAUCAGCAAACUACGUUUGGCUACUGUCUAGUUAGUUAAAAAGAGAUUUAUAGACUCGCUAUGUGAGGGCUUGAGUUAUGUGUCAUCACCUAGUUAAAUCAAUACAUAACCUAUAGUUGUAGACAAAUAUAUUAGCAGAUGUUUGG SEQ ID NO：217 
上面所提到的序列SEQ ID NO：47-62，65-68，71和72，98和99，159-168和176-217为新序列。 
上面所提到的序列能够用于形成ssrA基因序列的数据库，可用来鉴定细菌种类或产生核酸诊断检验盒。 
下面为与本发明相应的代表性探针： 
沙门氏菌： 
1)属特异性探针：： 
5’-CGAATCAGGCTAGTCTGGTAG-3’SEQ ID NO：218 
分枝杆菌： 
2)用于检测肺结核复合物的寡聚核苷酸探针： 
TB015’-ACTCCTCGGACA(A/G)CCACAGCGA-3’SEQ ID NO：219 
3)用于检测鸟分枝杆菌和鸟分枝杆菌(副结核亚种)序列的寡核苷酸探针 
探针1：PAV1-5’-GTTGCAAATAGATAAGCGCC-3’SEQ IDNO：220 
探针2：PAV2-5’-TCCGTCAGCCCGGGAACGCC-3’SEQID NO：221 
李斯特氏菌 
4)用于测定热杀灭处理细胞后tmRNA完整性的寡核苷酸探针 
LVtm：5′-TTTTGTTTTTCTTTGCCA-3′SEQ ID NO：222 
大肠杆菌： 
5)用于测定热杀死处理细胞后tmRNA完整性的寡核苷酸探针 
Evtm：5′-AGTTTTCGTCGTTTGCGA-3′SEQ ID NO：223 
下面为根据本发明的具代表性的引物： 
分枝杆菌： 
1)用于扩增所有分枝杆菌序列的简并引物 
5′引物 
10SAAM3-5′-CAGGCAA(G/C)(A/T/C)GACCACCGTAA-3′SEQ ID NO：224 
3′引物 
10SAAM4-5′GGATCTCC(C/T)G(A/G)TC(A/T)C(A/G)CG(A/G)AC(A/T)A-3′SEQ ID NO：225 
2)用于检测鸟分枝杆菌和鸟分枝杆菌(副结核亚种)序列的寡核苷酸引物 
5’引物：AP1正向-5’-TGCCGGTGCAGGCAACTG-3’SEQ ID NO：226 
3’引物：AP2反向-5’-CACGCGAACAAGCCAGGA-3’SEQ IDNO：227 
附图简述 
在附图中： 
图1为大肠杆菌和霍乱弧菌ssrA基因序列的Clustal对比； 
图2为从大肠杆菌和霍乱弧菌细胞中制备的全细胞RNA的琼脂糖凝胶照片； 
图3为霍乱弧菌寡核苷酸探针与大肠杆菌和霍乱弧菌的tmRNA的转录序列杂交的放射自显影照片； 
图4为用通用ssrA基因扩增引物从一系列生物体中得到的扩增产物的琼脂糖凝胶照片； 
图5为李斯特氏菌ssrA基因序列的Clustal对比； 
图6为单核细胞增多性李斯特氏菌和枯草芽孢杆菌ssrA/tmRNA基因序列的Clustal对比； 
图7为用李斯特菌属特异性PCR扩增引物从一系列生物体中得 到的扩增产物的琼脂糖凝胶照片； 
图8为李斯特菌属特异性寡核苷酸探针与实施例4中制备的一系列生物体杂交的放射自显影照片； 
图9为单核细胞增多性李斯特氏菌种特异性寡核苷酸探针与实施例7中制备的一系列生物体杂交的放射自显影照片； 
图10为如实施例6中所述的用多色探针检测方法检测尼龙膜上李斯特氏菌ssrA基因的计算机扫描图象； 
图11为沙眼衣原体菌株的ssrA基因序列的Clustal对比； 
图12为幽门螺杆菌菌株ssrA基因序列的Clustal对比； 
图13为生殖道枝原体菌株ssrA基因序列的Clustal对比； 
图14为淋病奈瑟球菌菌株ssrA基因序列的Clustal对比； 
图15为单核细胞增多性李斯特氏菌菌株ssrA基因序列的Clustal对比； 
图16为单核细胞增多性李斯特氏菌和无害李斯特菌菌株的ssrA基因序列的Clustal对比 
图17为李斯特菌寡聚核苷酸探针(Evtm)与经中度热处理的大肠杆菌细胞中分离的全RNA样品杂交的放射自显影照片； 
图18为李斯特菌寡聚核苷酸探针(Evtm)与经极度热处理的大肠杆菌细胞中分离的全RNA样品杂交的放射自显影照片； 
图19为李斯特氏菌寡聚核苷酸探针(Lvtm)与经中度热处理的单核细胞增多性李斯特氏菌细胞中分离的全RNA样品杂交的放射自显影照片； 
图20为李斯特氏菌寡聚核苷酸探针(Lvtm)与经极度热处理的单核细胞增多性李斯特氏菌细胞中分离的全RNA样品杂交的放射自显影照片； 
图21为经热处理后不同时间点tmRNA的RT-PCR产物的琼脂糖凝胶照片。 
本发明将通过下面的实施例作进一步的阐明。 
完成本发明的方式 
实施例1
检查可用的tmRNA序列的初级核苷酸序列
用Clustal W核酸序列对比程序对可用的tmRNA进行比较性初级核酸序列对比，表明原核生物的tmRNA比其它细菌高拷贝数RNA具有更显著程度的核酸序列变异性和非同源性，如表1所示； 
表1 
不同细菌的RNA分子间核苷酸序列同源性百分比 
    大肠杆菌∶霍乱弧菌   枯草芽孢杆菌∶结核分枝杆菌
  rRNA％同源性   88   66
  tmRNA％同源性   68   25
这些不同细菌的tmRNA序列间的非同源性区位于分子的中间，tmRNA分子内核苷酸序列的非同源性程度表明可制备属和种特异性探针以用来区别和/或检测细菌。 
核苷酸序列对比已经表明tmRNA分子的5’和3’侧翼区都有种内和属内的高度同源性。这表明可制备通用寡核苷酸引物，以用来从多种细菌中扩增ssrA基因或其编码的tmRNA。 
我们已经证明来源于不同生物体的tmRNA分子的核苷酸序列内的同源区和非同源区可以被用来作为在分子水平鉴定和检测生物体的基础。 
实施例2
霍乱弧菌tmRNA特异性探针的设计 
如图1所显示的大肠杆菌核苷酸序列(SEQ ID NO.37)和霍乱弧菌ssrA序列(SEQ ID NO.127)的对比表明这两种细菌在***发生上有紧密的联系。但是这些序列间的非同源区由缺乏星号标明。合成了图1 中加下划线的霍乱弧菌ssrA核苷酸序列可变区的互补的寡核苷酸探针。 
霍乱弧菌tmRNA特异性探针的序列为： 
5’-AACGAATGGCTAACCTGAA-3’SEQ ID NO.169 
在大肠杆菌和霍乱弧菌生长的中间指数期和稳定期，从液体培养物中分离得到全RNA。如图2所示等量的被分离的全RNA在变性甲醛琼脂糖凝胶中电泳并被印迹到HYBOND-N尼龙膜上，其中泳道1-4分别代表： 
泳道1：中间指数期大肠杆菌全RNA 
泳道2：中间指数期霍乱弧菌全RNA 
泳道3：稳定期大肠杆菌全RNA 
泳道4：稳定期霍乱弧菌全RNA 
将所得的Northern印迹与末端标记γP32的霍乱弧菌tmRNA特异性探针杂交。如图3所示的杂交试验的结果证明了探针的特异性，因为只有霍乱弧菌tmRNA被检测到。而且，观察到在生长稳定期的培养液中分离的霍乱弧菌tmRNA有更大程度的杂交信号强度，这表明在这一时期在霍乱弧菌细胞中存在更大拷贝数目的tmRNA分子。 
实施例3
用于表征未知ssrA基因和tmRNA序列的通用ssrA/tmRNA寡核苷酸扩增引物的产生
所有可用的ssrA基因和tmRNA序列的Clustal W对比表明能够设计出简并的寡核苷酸引物，用来从多种生物体中扩增ssrA基因和tmRNA核苷酸序列。 
可合成简并的寡核苷酸引物，用来从大范围的细菌的全基因组DNA制备物中PCR扩增得到ssrA基因序列。 
合成的简并寡核苷酸如下所示： 
(a)tmU5’：5’体外PCR扩增引物 
5’-GGG(A/C)(C/T)TACGG(A/T)TTCGAC-3’SEQ ID NO：170(b)tmU5’：3’体外PCR扩增引物 
5’-GGGA(A/G)TCGAACC(A/G)(C/G)GTCC-3’SEQ ID NO：171括号内为简并碱基位置。 
PCR反应的产物在琼脂糖凝胶中电泳，如图4所示在所有例子中部扩增得到约350碱基对PCR产物，这表明简并tmRNA引物的通用性。 
图4中的各泳道分别代表： 
A：分子量标记V 
1：大肠杆菌 
2：沙门氏菌浦纳亚种 
3：产气克雷伯氏菌 
4：奇异变形菌 
5：雷氏变形菌 
6：气单胞菌 
7：金黄色葡萄球菌 
8：肠球菌模式种(粪链球菌) 
9：乳杆菌模式种 
10：枯草芽孢杆菌 
11：单核细胞增多性李斯特氏菌 
12：无害李斯特菌 
13：墨氏李斯特菌 
14：威氏李斯特菌 
15：格氏李斯特菌 
16：牛分枝杆菌 
B：分子量标记V 
如表2所示，所述通用引物从革兰氏阳性和革兰氏阴性细菌中均扩增到了ssrA基因。 
表2
用通用扩增引物进行细菌物种检测 
Figure G00807503419920626D000881
实施例4
分离和表征以前未知的细菌ssrA/tmRNA核苷酸序列
由实施例2，从李斯特菌和牛分枝杆菌的基因组DNA中扩增得到的PCR产物被亚克隆到T末端质粒载体来进行DNA测序。每个物种挑选3个重组克隆并用双脱氧测序法进行测序。对每个亚克隆的DNA双链的序列进行测定。 
牛分枝杆菌ssrA基因的核苷酸序列与肺结核分枝杆菌ssrA基因序列有100％同源性。 
图5所示为从李斯特菌得到的新ssrA基因序列(SEQ ID NOS 51，53，55，59和61)的Clustal W对比。该分析表明能够产生用于李斯特菌的属特异性探针和寡核苷酸扩增引物。而且，该对比还表明可产生种特异性寡核苷酸探针，并能够从其它李斯特菌中区分出单核细胞增多性李斯特氏菌。 
在图5中，据推测的属特异性寡核苷酸引物Ltm1和Ltm2被框出，同时作为属特异性李斯特菌寡核苷酸探针的LGtM也被框出。据推测的单核细胞增多性李斯特氏菌种特异性寡核苷酸探针序列以下划线和斜体标识。 
为进一步说明ssrA基因/tmRNA核酸靶点为适合细菌诊断的靶点，进行了单核细胞增多性李斯特氏菌ssrA基因核苷酸序列(SEQ IDNO.55)和枯草芽孢杆菌ssrA基因核苷酸序列(SEQ ID NO.11)(一种和李斯特氏菌在种系发生上密切相关的细菌)的比较性对比，如图6所示。对该序列对比进行序列分析表明其序列同源性为41％，相应的16S rRNA对比表明核苷酸序列有87％的同源性(数据没有出示)。 
实施例5
用于李斯特氏菌菌种的ssrA基因/tmRNA属特异性扩增引物，属特异性和种特异性探针的产生和应用
用实施例4中李斯特氏菌的ssrA基因/tmRNA核苷酸序列对比，分析ssrA基因/tmRNA核苷酸序列区域以决定其是否适用于产生属特异性扩增引物和属特异性及种特异性寡核苷酸探针。在分析中，表现出与枯草芽孢杆菌有最大程度差异的区域被选出来设计这些扩增引物和探针。 
下面为合成的寡核苷酸的序列： 
(a)Ltm1：5’李斯特氏菌属特异性扩增引物 
5’-AAAGCCAATAATAACTGG-3’SEQ ID NO：172 
(b)Ltm2：3’李斯特氏菌属特异性扩增引物 
5’-CCAGAAATATCGGCACTT-3’SEQ ID NO：173 
(c)LGtm：李斯特氏菌属特异性杂交探针 
5’-GTGAGACCCTTACCGTAG-3’SEQ ID NO：174 
(d)LStm：单核细胞增多性李斯特氏菌种特异性杂交探针 
5’-TCTATTTAACCCCAGACG-3’SEQ ID NO：175 
属特异性扩增引物Ltm1和Ltm2用于与做为模板的二十个不同菌株的全基因组DNA进行一系列PCR反应。用这些引物只能从李斯特氏菌中扩增得到ssrA基因序列(260碱基对产物)(图7和表3)，这表明ssrA基因/tmRNA适合于作为体外扩增某细菌属的靶点。没有检测到其它细菌的扩增产物，尽管采用如实施例2所描述的通用引物(tmU5’和tmU3’)从这些细菌的DNA中PCR扩增得到了PCR产物。 
图7中的各泳道分别代表： 
A：分子量标记V 
1：大肠杆菌 
2：沙门氏菌浦纳亚种 
3：产气克雷伯氏菌 
4：奇异变形菌 
5：雷氏变形菌 
6：气单胞菌 
7：金黄色葡萄球菌 
8：肠球菌模式种 
9：乳杆菌模式种 
10：枯草芽孢杆菌 
11：单核细胞增多性李斯特氏菌菌株1 
12：单核细胞增多性李斯特氏菌菌株2 
13：单核细胞增多性李斯特氏菌菌株3 
14：单核细胞增多性李斯特氏菌菌株4 
15：单核细胞增多性李斯特氏菌临床分离物 
16：无害李斯特菌 
17：墨氏李斯特菌 
18：威氏李斯特菌 
19：格氏李斯特菌 
20：牛分枝杆菌 
B：分子量标记V 
表3 
用李斯特氏菌特异性扩增引物进行细菌种的检测 
如图4所示，李斯特氏菌属特异性寡核苷酸探针LGtM进行Southern印迹杂交。如图8和表4所示，只在各李斯特氏菌种中观察到阳性杂交信号，表明tmRNA序列可以作为检测特定属的靶点。 
图8中所示的各泳道为： 
A：分子量标记V 
1：大肠杆菌 
2：沙门氏菌浦纳亚种 
3：产气克雷伯氏菌 
4：奇异变形菌 
5：雷氏变形菌 
6：气单胞菌 
7：金黄色葡萄球菌 
8：肠球菌模式种 
9：乳杆菌模式种 
10：枯草芽孢杆菌 
11：单核细胞增多性李斯特氏菌 
12：无害李斯特菌 
13：墨氏李斯特菌 
14：威氏李斯特菌 
15：格氏李斯特菌 
16：牛分枝杆菌 
B：分子量标记V 
用本实施例所描述的属特异性扩增得到的PCR产物，如图7所示，进行Southern印迹，其与单核细胞增多性李斯特氏菌种特异性寡核苷酸探针杂交。如图9和表4所示在四株单核细胞增多性李斯特氏菌中的三株和一株临床分离得到的菌株检测到阳性杂交信号。 
图9中的各泳道代表： 
A：分子量标记V 
1：大肠杆菌 
2：沙门氏菌浦纳亚种 
3：产气克雷伯氏菌 
4：奇异变形菌 
5：雷氏变形菌 
6：气单胞菌 
7：金黄色葡萄球菌 
8：肠球菌模式种 
9：乳杆菌模式种 
10：枯草芽孢杆菌 
11：单核细胞增多性李斯特氏菌菌株1 
12：单核细胞增多性李斯特氏菌菌株2 
13：单核细胞增多性李斯特氏菌菌株3 
14：单核细胞增多性李斯特氏菌菌株4 
15：单核细胞增多性李斯特氏菌临床分离物 
16：无害李斯特菌 
17：墨氏李斯特菌 
18：威氏李斯特菌 
19：格氏李斯特菌 
20：牛分枝杆菌 
B：分子量标记V 
表4
李斯特氏菌属特异性探针和单核细胞增多性李斯特氏菌种特异性探针的特异性
Figure G00807503419920626D000941
菌株4，一种典型的单核细胞增多性李斯特氏菌菌株，用这种探针不能产生阳性信号。对从该菌株中PCR扩增得到的ssrA基因进行DNA测序表明其含有与无害李斯特氏菌相同的探针靶点区。但是应该注意到从该菌株得到的ssrA基因包含的其它区域的序列与前述的单核 细胞增多性李斯特氏菌中的这些序列相同而不同于无害李斯特氏菌，如图15所示。所以可合成针对这些可变区其中之一的种特异性寡核苷酸探针，该探针或在种(例如单核细胞增多性李斯特氏菌)内识别每种株型(分离物)。 
实施例6
李斯特氏菌ssrA基因序列的多色探针检测
将LGTm(A)，LStm(B)和乌普萨拉弯曲杆菌的16S-23S rRNA间隔序列(C-5′CATTAAACTTTAGCAAGGAAGTG 3′SEQ ID NO：228)寡核苷酸探针非可逆地结合到尼龙膜上与扩增的ssrA PCR产物进行杂交，所述扩增利用属特异性引物Ltm1and Ltm2(Ltm1的5′端标记有生物素)，它们来源于单核细胞增多性李斯特氏菌(1-6)，无害李斯特菌(7-10)，伊氏李斯特氏菌(11)，墨氏李斯特氏菌(12)，斯氏李斯特氏菌(13)，威氏李斯特氏菌(14)和格氏李斯特氏菌(15)。用tmU5′和tmU3′(tmU5′的5′端标记有生物素)扩增得到的ssrA的PCR扩增产物也被杂交到尼龙膜条带上，被检测细菌来源于革兰氏阳性菌枯草芽孢杆菌、乳杆菌模式种、金黄色葡萄球菌、表皮葡萄球菌、肠球菌模式种、产气荚膜梭菌(16-21)和革兰氏阴性菌大肠杆菌、肠炎沙门氏菌、雷氏变形菌、产气克雷伯氏菌(22-25)。如图10所示，经杂交后，对生物素比色检验进行显影表明：条带1-6表明ssrA的PCR扩增产物来自于单核细胞增多性李斯特氏菌并证明PCR扩增产物来自李斯特氏菌，因A和B都能够进行颜色检测；条带7-15证明PCR产物来自于李斯特氏菌，但其中只有A能够进行颜色检测；条带16-25表明PCR产物不是从李斯特氏菌中得到的，因没有检测到颜色。C是阴性寡核苷酸对照探针而D为对所有样品的比色检验的阳性对照。 
实施例7
用ssrA/tmRNA序列来区分生物体的物种
图11(SEQ ID NOS.19和21)，图12(SEQ ID NOS.41和43)，图13(SEQ ID NOS.77和79)，图14(SEQ ID NOS.83和85)15和16 (SEQ ID NO.53，55和57)所示的Clustal W序列对比表明在同一种细菌的不同菌株的ssrA/tmRNA序列内存在着核苷酸的差异。这表明ssrA/tmRNA序列潜在地可以用来区分同一细菌种类中不同菌株的个体和/或集体。这有助于其应用于流行病学研究和细菌种群分析。 
实施例8
对细菌细胞进行中度和极度热处理后分析tmRNA的完整性
对培养物进行热处理：将大肠杆菌和单核细胞增多性李斯特氏菌在80℃进行热处理，对于大肠杆菌而言处理20分钟，对于单核细胞增多性李斯特氏菌处理40分钟，以及于120℃处理15分钟(高压釜)。经过过夜生长和热处理后在0小时，1小时，2小时，6小时，12小时和48小时进行测验。每个时期的检测都没观察到存活的菌体。在这些时期全RNA被分离并在变性的1.2％琼脂糖凝胶中电泳及进行Northern印迹。在大肠杆菌的例子中(图17和图18)每个印迹都会与放射性标记的寡核苷酸探针Evtm杂交，在单核细胞增多性李斯特氏菌例子中(图19和图20)印迹与放射性标记的LVtm杂交。在每个检测样品中没有检测到tmRNA转录序列，这表明热处理后tmRNA转录序列已被降解。带有符号+ve的泳道为全RNA样品的阳性对照。 
实施例9
用tmRNA转录序列来区别活的和死的细菌
100ml单核细胞增多性李斯特氏菌在液体培养液中培养过夜。生长后，对细胞进行连续稀释并通过在营养琼脂平板上铺板来测定存活率。同时，从这些细胞的1ml液体中分离得到全RNA。剩余的细胞在65℃热处理20分钟。接着将细胞除去来进行存活率分析和全RNA分离。在处理后0小时，2小时，6小时和24小时的时间点取样品来测定存活率和RNA分离。 
在营养琼脂平板上铺板表明热处理能杀死单核细胞增多性李斯特氏菌细胞，因没有观察到活的集落形成单位。每个分离的RNA样品 用DNA酶处理以除去所含的任何DNA并且用李斯特氏菌属特异性ssrA/tmRNA寡核苷酸引物Ltm1和Ltm2对全RNA样品(100ng)进行反转录PCR扩增。扩增反应的阴性对照包括引物、靶序列和Taq聚合酶，但没有反转录酶。 
扩增反应的结果如图12所示。 
扩增的tmRNA的RT-PCR产物只能在没有经热处理的RNA样品中检测到。所有其它样品中没有产生RT-PCR产物，这表明这些样品中tmRNA分子在热处理后死的细胞中已经被降解。 
图21中的各泳道代表： 
A：分子量标记V； 
1：RNA的PCR扩增(没有热处理过的细胞) 
   反转录酶(RT)，+Taq聚合酶(TP)； 
2：RNA的RT-PCR(没有经过热处理的细胞)，+RT，+TP； 
3：RNA的PCR扩增(热处理后0小时)，-RT，+TP； 
4：RNA的RT-PCR(热处理后0小时)，+RT，+TP； 
5：RNA的PCR扩增(热处理后1小时)，-RT，+TP； 
6：RNA的RT-PCR(热处理后1小时)，+RT，+TP； 
7：RNA的PCR扩增(热处理后2小时)，-RT，+TP； 
8：RNA的RT-PCR(热处理后2小时)，+RT，+TP； 
9：RNA的PCR扩增(热处理后6小时)，-RT，+TP； 
10：RNA的RT-PCR(热处理后6小时)，+RT，+TP； 
11：RNA的PCR扩增(热处理后24小时)，-RT，+TP； 
12：RNA的RT-PCR  (热处理后24小时)，+RT，+TP； 
B：分子量标记 
Figure K00807503419920626Z000011
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Figure K00807503419920626Z000081
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Figure K00807503419920626Z000101
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Figure K00807503419920626Z000141
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Figure K00807503419920626Z000681

Claims (23)

1.ssrA基因分子中与低同源区相对应的片段的用途，其作为用于原核生物或真核生物的核酸探针检验中的靶点区，其中该用途用于非疾病诊断目的。
2.根据权利要求1的用途，其中ssrA基因分子中与低同源区相对应的片段作为核酸探针检验中的靶点区用于区分不同物种。
3.根据权利要求1的用途，其中ssrA基因分子中与低同源区相对应的片段作为靶点区用于产生属特异性探针。
4.权利要求1-3中任何一项的用途，其中所述ssrA基因分子的片段被用作为在扩增中所用的引物的基础。
5.根据权利要求4的用途，其中扩增产物被用来作为核酸探针检验的靶点区。
6.根据权利要求1-3中任何一项所述的用途，其中所述ssrA基因的片段被用于得到原核生物或真核生物的DNA图谱的检验中，从而区分同一物种的不同株系。
7.根据权利要求1-3中任何一项所述的用途，其中所述ssrA基因的片段被用来设计用于治疗目的来对抗感染性的原核生物或真核生物的试剂。
8.tmRNA分子中与低同源区相对应的片段的用途，其作为用于原核生物或真核生物的核酸探针检验中的靶点区，其中该用途用于非疾病诊断目的。
9.根据权利要求8的用途，其中tmRNA分子中与低同源区相对应的片段作为核酸探针检验中的靶点区用于区分不同物种。
10.根据权利要求8的用途，其中tmRNA分子中与低同源区相对应的片段作为靶点区用于产生属特异性探针。
11.权利要求8-10中任何一项的用途，其中所述tmRNA分子的片段被用作为在扩增中所用的引物的基础。
12.根据权利要求11的用途，其中扩增产物被用来作为核酸探针检验的靶点区。
13.根据权利要求8-10中任何一项所述的用途，其中所述tmRNA分子片段的cDNA转录序列被用作为核酸杂交检验中的探针。
14.权利要求1-3和8-10中任何一项所述的用途，其中所述的检验在体外完成。
15.根据权利要求1-3和8-10中任何一项所述的用途，其中所述的检验在原位完成。
16.根据权利要求1-3和8-10中任何一项所述的用途，其中的靶点区被作为用于区分活的和死的原核生物或真核生物的检验的基础。
17.根据权利要求1-3和8-10中任何一项所述的用途，其中的靶点区被用于多探针形式以大范围地检测和/或鉴定原核生物或真核生物。
18.根据权利要求17的用途，其中所述探针被连接到微阵列基因芯片***上，用于大范围地高通量检测和鉴定原核生物或真核生物。
19.权利要求1-3和8-10中任何一项所述的用途，其中的靶点区被用作为用于检测目标样品中原核生物或真核生物的检验中的探针。
20.根据权利要求8-10中任何一项所述的用途，其中所述tmRNA的片段被用于得到原核生物或真核生物的DNA图谱的检验中，从而区分同一物种的不同株系。
21.根据权利要求8-10中任何一项所述的用途，其中所述tmRNA转录序列的片段被用来设计用于治疗目的来对抗感染性的原核生物或真核生物的试剂。
22.根据权利要求1-3和8-10中任何一项所述的用途，其中所述检验被用于原核生物或真核生物的定量分析。
23.根据权利要求1-3中任何一项所述的用途，其中所述ssrA基因片段的数据库被用来鉴定原核生物或真核生物。
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