CN1291231C - 活化淋巴细胞特异性的检测方法 - Google Patents
活化淋巴细胞特异性的检测方法 Download PDFInfo
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
Description
有排斥反应发生时 | A、B两组与C、D两组相比,细胞活性明显减弱,尤其是B组,细胞活性可完全被抑制,A组细胞活性减弱的程度可能与排斥反应强弱有关,排斥反应越强减弱越明显,排斥反应弱时,A组与C、D组比活性减弱不明显;但只要有排斥反应,B组细胞活性就会比C组明显减弱。 |
无排斥反应 | 各组细胞活性无明显差别或C、B组均较弱,有时C更弱。 |
无排斥反应但最近或目前正在发生细菌或病毒感染 | 各组细胞活性均较强,各组之间亦无明显差别,或C组稍减弱。 |
最近或目前正在发生细菌或病毒感染,同时有排斥反应发生 | A组或有减弱,B组的细胞活性比C组及A、D组的细胞活性明显减弱。D组的细胞活性很强。 |
活检 | |||
阳性 | 阴性 | ||
本实验结果 | 阳性阴性 | 180 | 1127 |
有结核杆菌感染 | A、B两组与C、D两组相比,细胞活性明显减弱,尤其是B组,细胞活性可完全被抑制,A组细胞活性减弱的程度可能与特异性活化淋巴细胞的多少有关,特异性活化淋巴细胞越多减弱越明显,否则A组与C、D组比活性减弱不明显;但只要有抗原特异性活化淋巴细胞,B组细胞活性就会比C组明显减弱。 |
无结核杆菌感染 | 各组细胞活性无明显差别或C、B组较弱 |
无结核杆菌感染,但最近或目前正在发生其它细菌或病毒感染 | 各组细胞活性均较强,各组之间亦无明显差别,或C组稍减弱。 |
最近或目前正在发生细菌或病毒感染,同时有结核杆菌感染 | A组或有减弱,B组的细胞活性比C组及A、D组的细胞活性明显减弱。A、C、D组细胞活性均很强。 |
Claims (23)
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CNB2003101198721A CN1291231C (zh) | 2003-12-08 | 2003-12-08 | 活化淋巴细胞特异性的检测方法 |
DE602004029194T DE602004029194D1 (de) | 2003-12-08 | 2004-12-07 | Methode zur messung der spezifizitat aktivierter lymphozyten |
AT04802441T ATE481638T1 (de) | 2003-12-08 | 2004-12-07 | Methode zur messung der spezifizit t aktivierter lymphozyten |
EP04802441A EP1710580B1 (en) | 2003-12-08 | 2004-12-07 | A method of assaying specificity of activated lymphocytes |
RU2006124531/13A RU2343486C2 (ru) | 2003-12-08 | 2004-12-07 | Способ детектирования специфичности активированных лимфоцитов и среда для его осуществления |
PCT/CN2004/001427 WO2005083427A1 (fr) | 2003-12-08 | 2004-12-07 | Procede d'essai de specification de l'activation de lymphocytes |
JP2006541786A JP4444965B2 (ja) | 2003-12-08 | 2004-12-07 | 活性化リンパ球の特異性の検出方法 |
US10/582,056 US7829302B2 (en) | 2003-12-08 | 2004-12-07 | Method for detecting the specificity of activated lymphocyte |
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CNB2003101198721A CN1291231C (zh) | 2003-12-08 | 2003-12-08 | 活化淋巴细胞特异性的检测方法 |
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EP (1) | EP1710580B1 (zh) |
JP (1) | JP4444965B2 (zh) |
CN (1) | CN1291231C (zh) |
AT (1) | ATE481638T1 (zh) |
DE (1) | DE602004029194D1 (zh) |
RU (1) | RU2343486C2 (zh) |
WO (1) | WO2005083427A1 (zh) |
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JP2009028011A (ja) * | 2007-07-30 | 2009-02-12 | Kao Corp | 細胞呼吸活性の測定方法及びそのキット、タンパク質生産性向上株のスクリーニング方法及びそのキット |
RU2450274C1 (ru) * | 2010-12-24 | 2012-05-10 | Федеральное государственное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФГУН ГНЦ ВБ "Вектор" Роспотребнадзора) | Способ ранней диагностики заболевания геморрагической лихорадкой эбола у лиц, предположительно инфицированных указанным вирусом |
CN104483376A (zh) * | 2014-12-30 | 2015-04-01 | 青岛市市立医院 | 一种筛选环孢素a于免疫t细胞中药物作用靶位的方法 |
WO2019002689A1 (en) | 2017-06-27 | 2019-01-03 | Thermo Fisher Scientific Oy | METHOD FOR ANALYZING LIQUID SAMPLES, MICRO-PLATE READER AND COMPUTER PROGRAM |
WO2019172419A1 (ja) * | 2018-03-09 | 2019-09-12 | 株式会社マンダム | 免疫関連疾患の指標の検出方法 |
CN110804643B (zh) * | 2019-10-29 | 2022-06-21 | 同济大学 | 一种体外评价卡介苗对中性粒细胞活性影响的方法 |
CN117741162A (zh) * | 2024-02-20 | 2024-03-22 | 苏州才博医学科技有限公司 | 一种检测供者特异性抗体的细胞学方法 |
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US4808151A (en) | 1987-04-27 | 1989-02-28 | E. I. Du Pont De Nemours And Company | Simplified method for the preparation of human lymphokine activated killer cells |
US5714585A (en) | 1987-10-26 | 1998-02-03 | Sterling Winthrop, Inc. | Antibodies that are immunoreactive with interleukin-7 |
WO1993014780A1 (en) * | 1992-01-28 | 1993-08-05 | Abraham Karpas | The use of a compound for the manufacture of a medicament for the treatment of hiv infection |
CN1112933A (zh) * | 1994-01-20 | 1995-12-06 | 伯伦格·曼海姆有限公司 | 抗原特异性活化t淋巴细胞,其检测和用途 |
US6150121A (en) * | 1999-02-16 | 2000-11-21 | Wisconsin Alumni Research Foundation | Assessing immunological state of transplant recipients |
US6346247B1 (en) * | 1999-10-28 | 2002-02-12 | Promega Corporation | Prevention and treatment of autoimmune disease with luminally administered polyclonal antibodies |
WO2003044529A1 (en) | 2001-11-16 | 2003-05-30 | Rigel Pharmaceuticals, Inc. | Modulators of lymphocyte activation, mkk3b compositions and methods of use |
US20030211548A1 (en) | 2002-01-29 | 2003-11-13 | Oncolmmunin, Inc. | Visualization and quantitiation of cellular cytotoxicity using cell-permeable fluorogenic protease substrates and caspase activity indicator markers |
DE50207025D1 (de) * | 2002-08-23 | 2006-07-06 | Deutsches Rheuma Forschungszen | Verfahren zum Nachweis und zur Isolierung von T-Lymphozyten, die ein definiertes Antigen erkennen |
CN1444043A (zh) * | 2002-12-25 | 2003-09-24 | 帕弗瑞生物技术(北京)有限公司 | 一种个体化特异性免疫细胞功能测定方法 |
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- 2004-12-07 WO PCT/CN2004/001427 patent/WO2005083427A1/zh active Application Filing
- 2004-12-07 EP EP04802441A patent/EP1710580B1/en not_active Not-in-force
- 2004-12-07 DE DE602004029194T patent/DE602004029194D1/de active Active
- 2004-12-07 JP JP2006541786A patent/JP4444965B2/ja not_active Expired - Fee Related
- 2004-12-07 AT AT04802441T patent/ATE481638T1/de not_active IP Right Cessation
- 2004-12-07 US US10/582,056 patent/US7829302B2/en not_active Expired - Fee Related
- 2004-12-07 RU RU2006124531/13A patent/RU2343486C2/ru not_active IP Right Cessation
Also Published As
Publication number | Publication date |
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ATE481638T1 (de) | 2010-10-15 |
JP4444965B2 (ja) | 2010-03-31 |
EP1710580A4 (en) | 2008-03-12 |
RU2343486C2 (ru) | 2009-01-10 |
JP2007512816A (ja) | 2007-05-24 |
US7829302B2 (en) | 2010-11-09 |
EP1710580A1 (en) | 2006-10-11 |
EP1710580B1 (en) | 2010-09-15 |
DE602004029194D1 (de) | 2010-10-28 |
US20070172448A1 (en) | 2007-07-26 |
RU2006124531A (ru) | 2008-01-20 |
WO2005083427A1 (fr) | 2005-09-09 |
CN1627074A (zh) | 2005-06-15 |
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