CN1287809C - Method of extracting total flavone product from halenalin plant full flower - Google Patents
Method of extracting total flavone product from halenalin plant full flower Download PDFInfo
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- CN1287809C CN1287809C CN 200410012598 CN200410012598A CN1287809C CN 1287809 C CN1287809 C CN 1287809C CN 200410012598 CN200410012598 CN 200410012598 CN 200410012598 A CN200410012598 A CN 200410012598A CN 1287809 C CN1287809 C CN 1287809C
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Abstract
The present invention relates to a method for extracting total flavonoid products from the whole flowers of caragana plants, which comprises the steps of preparing the column liquid of the whole flowers of the caragana plants, separating by a separation column, eluting the separation column by an eluting agent and concentrating eluting liquid, wherein the step of preparing the column liquid of the whole flowers of the caragana plants comprises: grinding the dried whole flowers of the caragana plants, sieving by a sieve of which the sieve size is from 40 to 60 meshes, adding the dried powder to a 40 to 70% ethanol solution in the liquid to solid ratio of 6 to 25:1, stirring at the temperature of 40 to 65 DEG C, soaking for 2 to 4 hours, filtering, adding the filtering residues to a 40 to 70% ethanol solution in the liquid to solid ratio of 4 to 12:1, stirring at the temperature of 40 to 65 DEG C, soaking for 1 to 2 hours, extracting for 2 times, and then merging filtering liquid obtained through filtering for 3 times to obtain the column liquid; separating by the separation column, eluting, and then concentrating to obtain the total flavonoid products. The present invention has the advantage of reasonable, accurate, simple and easily executed extraction method, and the output rate of the total flavonoid products extracted from the whole flowers of the caragana plants is high and can reach 2 to 3% so as to reduce extraction cost. The present invention expands the raw material sources of the total flavonoid products.
Description
Technical field
The present invention relates to from plant, extract the method for total flavones product, be specially the method for from the population pattern change whole flower, extracting the total flavones product.
Background technology
Flavone compound is a class flavochrome that extensively is present in the plant, its physiological function that presents is varied, comprise cardiovascular function, anti-oxidation function, arrhythmia function, the anticancer function of antitumor, antitussive and antiasthmatic function, anti-bacteria and anti-virus function, blood pressure lowering blood fat function, parahormone sample function and immunoloregulation function etc., have a wide range of applications in food and medicine industry.
The extraction industry of flavone compound at present mainly faces following problem: 1, the vegetable raw material of scale extraction mainly concentrates on Folium Ginkgo, Radix Et Rhizoma Fagopyri Tatarici seed, Semen sojae atricolor etc.Wherein Folium Ginkgo total flavones content for the highest, reaches as high as 2.74% in giving birth to spire then, on average be about 1.3%, but spire output is limited, and the general flavone content among the Lao Ye has greatly limited the extracted amount of total flavones product mostly less than 1%.If as the extraction raw material of flavone compound, the plantation of Radix Et Rhizoma Fagopyri Tatarici and Semen sojae atricolor will certainly take this arable land in short supply, and the plantation management cost is higher.When 2, from the leaf of plant, extracting the total flavones product, need usually to remove lipophilic impurity such as chlorophyll, make the total flavones product be difficult to purify with chloroform, petroleum ether and even kerosene.
Population pattern change (Caragana Fabr) is a machaka, is distributed widely in desert, oasis or the Loess Region of northern China arid, semiarid zone, is vanguard tree seed and high-quality livestock feed that northern area is planted trees, checked winds and fixed drifting sand.In China, generally its cultigen is commonly referred to as Herba Caraganae intermediae.Measure discovery through chemical examination, the population pattern change whole flower has higher general flavone content (about 3%) in (comprising each position of bennet, calyx, petal, stamen and gynoecium), and contained chlorophyll and other oil-soluble impurities are seldom, need not decolour in the leaching process, can reduce the residual of extraction cost and noxious substance.Because population pattern change belongs to the quick growing species of trees, and lifes in 3 years and later plant branch are luxuriant spends numerously, and therefore the colored output as total flavones product extraction raw material is very high.
The preparation, upper prop liquid that the existing conventional method of extracting the total flavones product from plant comprises upper prop liquid separated, detached dowel carried out eluting and eluent is concentrated with eluant by detached dowel.Because the population pattern change whole flower is a kind of raw material of newfound extraction total flavones, its extracting method, different and the existing conventional method of from plant, extracting the total flavones product of the specific technical parameter in the extracting method particularly, adopt the existing conventional method of from plant, extracting the total flavones product to be difficult to guarantee the output capacity of higher total flavones product, make the extraction cost of total flavones product higher.
Summary of the invention
The present invention solves the existing conventional method of total flavones product of extracting at a kind of new extraction raw material---population pattern change whole flower from plant, some specific technical parameters are difficult to select, make the lower problem of output capacity of its total flavones product, provide a kind of output capacity the higher method of from the population pattern change whole flower, extracting the total flavones product.
The present invention adopts following technical scheme to realize: the method for extracting the total flavones product from the population pattern change whole flower, comprise the preparation of population pattern change whole flower upper prop liquid, separate by detached dowel, detached dowel is carried out eluting and eluent is concentrated with eluant, the preparation of population pattern change whole flower upper prop liquid is that dried population pattern change whole flower is pulverized, cross the 40-60 mesh sieve, with dried powder with 6-25: 1 liquid material is than adding in the 40-70% alcoholic solution, 40-65 ℃ is stirred down and soaked 2-4 hour, filter, filtering residue is again with 4-12: 1 liquid material is than adding in the 40-70% alcoholic solution, 40-65 ℃ is stirred down and soaked 1-2 hour, extract 2 times, three filtrate merging are obtained upper prop liquid; Upper prop liquid reclaims ethanol at 50-65 ℃ of following concentrating under reduced pressure, does not have alcohol to upper prop liquid and distinguishes the flavor of; Upper prop liquid separates through the macroporous adsorbent resin detached dowel then; It is colourless with distilled water detached dowel to be eluted to eluent, and it is colourless that the alcoholic solution of reuse 40-70% is eluted to eluent; Eluent merges, and behind 50-65 ℃ of following decompression recycling ethanol, vacuum drying obtains the total flavones product.
But the drying natural air drying of population pattern change whole flower, or at 40-60 ℃ of following constant temperature drying.
The method of the invention is preferably to draw on the basis of a large amount of experiments, and its extracting method is reasonable, accurate, simple and easy to do, extracts the total flavones product and have than high yield from the population pattern change whole flower, can reach 2-3%, thereby have lower extraction cost.The present invention has enlarged the raw material sources of total flavones product for the utilization of population pattern change whole flower provides the foundation.
The specific embodiment
With littleleaf peashrub (Caragana microphylla Lam) or Caragana intermedia (C.intermediaKuanget H.C.Fu) is example.
Littleleaf peashrub (or Caragana intermedia) whole flower of natural air drying (or 50 ℃, 60 ℃ constant temperature dryings) is pulverized, crossed 40 orders (or 50 orders or 60 orders) sieve; Take by weighing 1 kilogram of dried powder after sieving, add in 6-25 liter (optional 10,15,20 liters) 45% (or 50% or 55% or the 60% or 65%) alcoholic solution, stir under 50 ℃ (or 45 ℃ or 55 ℃ or 60 ℃ or 65 ℃) and soaked 3 hours, filter, filtering residue adds in 4-12 liter (optional 5,8,10 liters) 45% (or 50% or 55% or the 60% or 65%) alcoholic solution again, stir under 50 ℃ (or 45 ℃ or 55 ℃ or 60 ℃ or 65 ℃) and soaked 1 hour, extract 2 times, three filtrate merging are obtained upper prop liquid; Upper prop liquid concentrating under reduced pressure under 60 ℃ (55 ℃ or 65 ℃) reclaims ethanol, does not have alcohol to upper prop liquid and distinguishes the flavor of; Upper prop liquid separates through macroporous adsorbent resin (selecting CAD-80 for use) detached dowel then; It is colourless with distilled water detached dowel to be eluted to eluent, and it is colourless that the alcoholic solution of reuse 45% (or 50% or 55% or 60% or 65%) is eluted to eluent; Eluent merges, and behind the decompression recycling ethanol, 40-60 ℃ of vacuum drying obtains total flavones product 64.76 grams under 50 ℃ (or 55 ℃ or 65 ℃), and its general flavone content is 37.31% after measured.
Claims (2)
1, a kind of method of from the population pattern change whole flower, extracting the total flavones product, comprise the preparation of population pattern change whole flower upper prop liquid, separate by detached dowel, detached dowel is carried out eluting and eluent is concentrated with eluant, it is characterized by: the preparation of population pattern change whole flower upper prop liquid is that dried population pattern change whole flower is pulverized, cross the 40-60 mesh sieve, with dried powder with 6-25: 1 liquid material is than adding in the 40-70% alcoholic solution, 40-65 ℃ is stirred down and soaked 2-4 hour, filter, filtering residue is again with 4-12: 1 liquid material is than adding in the 40-70% alcoholic solution, 40-65 ℃ is stirred down and soaked 1-2 hour, extract 2 times, three filtrate merging are obtained upper prop liquid; Upper prop liquid reclaims ethanol at 50-65 ℃ of following concentrating under reduced pressure, does not have alcohol to upper prop liquid and distinguishes the flavor of; Upper prop liquid separates through the macroporous adsorbent resin detached dowel then; It is colourless with distilled water detached dowel to be eluted to eluent, and it is colourless that the alcoholic solution of reuse 40-70% is eluted to eluent; Eluent merges, and behind 50-65 ℃ of following decompression recycling ethanol, vacuum drying obtains the total flavones product.
2, the method for extracting the total flavones product from the population pattern change whole flower as claimed in claim 1 is characterized by: but the drying natural air drying of population pattern change whole flower, or at 40-60 ℃ of following constant temperature drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410012598 CN1287809C (en) | 2004-10-16 | 2004-10-16 | Method of extracting total flavone product from halenalin plant full flower |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410012598 CN1287809C (en) | 2004-10-16 | 2004-10-16 | Method of extracting total flavone product from halenalin plant full flower |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1650908A CN1650908A (en) | 2005-08-10 |
| CN1287809C true CN1287809C (en) | 2006-12-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200410012598 Expired - Fee Related CN1287809C (en) | 2004-10-16 | 2004-10-16 | Method of extracting total flavone product from halenalin plant full flower |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105998154B (en) * | 2016-06-16 | 2021-04-09 | 辽宁大学 | Preparation method of total flavonoids in narrow-leaved vetch |
| CN111840359A (en) * | 2020-07-15 | 2020-10-30 | 新疆医科大学 | Application of total flavones of broom cypress in preparation of medicine for treating renal hypertension |
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Granted publication date: 20061206 Termination date: 20091116 |