CN116531418B - Method for extracting flavonoid substances from pistacia chinensis bunge - Google Patents
Method for extracting flavonoid substances from pistacia chinensis bunge Download PDFInfo
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- CN116531418B CN116531418B CN202310625533.8A CN202310625533A CN116531418B CN 116531418 B CN116531418 B CN 116531418B CN 202310625533 A CN202310625533 A CN 202310625533A CN 116531418 B CN116531418 B CN 116531418B
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- 235000014123 Pistacia chinensis Nutrition 0.000 title claims abstract description 85
- 240000000432 Pistacia chinensis Species 0.000 title claims abstract description 85
- 229930003935 flavonoid Natural products 0.000 title claims abstract description 32
- 235000017173 flavonoids Nutrition 0.000 title claims abstract description 32
- 150000002215 flavonoids Chemical class 0.000 title claims abstract description 27
- 239000000126 substance Substances 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000002386 leaching Methods 0.000 claims abstract description 65
- 239000000706 filtrate Substances 0.000 claims abstract description 63
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000000284 extract Substances 0.000 claims abstract description 45
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 claims abstract description 36
- 238000002156 mixing Methods 0.000 claims abstract description 27
- 239000000843 powder Substances 0.000 claims abstract description 24
- JMMWKPVZQRWMSS-UHFFFAOYSA-N isopropanol acetate Natural products CC(C)OC(C)=O JMMWKPVZQRWMSS-UHFFFAOYSA-N 0.000 claims abstract description 19
- 229940011051 isopropyl acetate Drugs 0.000 claims abstract description 19
- GWYFCOCPABKNJV-UHFFFAOYSA-N isovaleric acid Chemical compound CC(C)CC(O)=O GWYFCOCPABKNJV-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 45
- 239000011259 mixed solution Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 15
- -1 flavonoid compounds Chemical class 0.000 abstract description 5
- 239000012065 filter cake Substances 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 3
- 238000003756 stirring Methods 0.000 description 22
- 230000000052 comparative effect Effects 0.000 description 11
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 235000003445 Pistacia Nutrition 0.000 description 1
- 241000543704 Pistacia Species 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 244000044283 Toxicodendron succedaneum Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/22—Anacardiaceae (Sumac family), e.g. smoketree, sumac or poison oak
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention relates to the technical field of natural medicine extraction, and provides a method for extracting flavonoid substances from pistacia chinensis bunge, which comprises the following steps of: s1: mixing pistacia chinensis bunge stem and leaf powder with a first leaching solution, heating and leaching, and filtering to obtain filtrate and filter residues, wherein the first leaching solution is methanol solution; s2: mixing the filter cake obtained in the step S2 with a second leaching solution, and carrying out countercurrent leaching to obtain filtrate and filter residue, wherein the second leaching solution is isopropyl acetate and/or butanone; s3: mixing the first filtrate with the second filtrate, distilling under reduced pressure, and lyophilizing to obtain Pistacia chinensis extract. By the technical scheme, the problems of technical blank and the like in the related technology for extracting flavonoid compounds from pistacia chinensis bunge are solved.
Description
Technical Field
The invention relates to the technical field of natural medicine extraction, in particular to a method for extracting flavonoid substances from pistacia chinensis bunge.
Background
Flavonoid compounds are widely used in various plants, and have a wide variety and a variety of structures. It has been reported that intake of flavonoids in an appropriate amount can reduce the incidence of cancer, tumor, cardiovascular disease, lipid peroxidation, osteoporosis, etc. Because of its high medicinal value, there is an increasing research on flavonoids.
Pistacia chinensis bunge, etc., belongs to the genus pistacia of the family lacquer tree. The tree species with temperate zone, happiness and light, strong adaptability, developed root system and long service life are widely distributed in China. Pistacia chinensis has high medicinal value, and can be used as medicine for root, branch, bark and leaf. However, the extraction of flavonoids from pistacia chinensis bunge has not been reported.
Disclosure of Invention
The invention provides a method for extracting flavonoid substances from pistacia chinensis bunge, which solves the problems of technical blank of extracting flavonoid compounds from pistacia chinensis bunge in the related technology.
The technical scheme of the invention is as follows:
a method for extracting flavonoid substances from pistacia chinensis bunge comprises the following steps:
s1: mixing pistacia chinensis bunge stem and leaf powder with a first leaching solution, heating and leaching, and filtering to obtain a first filtrate and first filter residues, wherein the first leaching solution is methanol solution;
s2: mixing the first filter residue with a second leaching solution, and carrying out countercurrent leaching to obtain a second filtrate and a second filter residue, wherein the second leaching solution is isopropyl acetate and/or butanone;
s3: mixing the first filtrate and the second filtrate, distilling under reduced pressure, and lyophilizing to obtain Pistacia chinensis extract.
As a further technical scheme, the pistacia chinensis bunge stem leaf powder is obtained by the following method:
washing, drying and crushing pistacia chinensis bunge stems and leaves to obtain pistacia chinensis bunge stem and leaf powder, wherein the particle size of pistacia chinensis bunge stem and leaf powder is 0.5-1cm.
As a further technical scheme, the volume fraction of the methanol solution is 30-40%.
As a further technical scheme, the feed liquid ratio of the pistacia chinensis bunge stem leaf powder to the first leaching solution is 1:100-300g/mL.
As a further technical scheme, the heating leaching temperature in the step S1 is 35-45 ℃ and the time is 4-5h.
As a further technical scheme, the second leaching solution is a mixed solution of isopropyl acetate and butanone in a volume ratio of 2-5:5.
As a further technical scheme, the feed liquid ratio of the pistacia chinensis bunge stem leaf powder to the second leaching solution is 1:50-100g/mL.
As a further technical scheme, the countercurrent leaching temperature in the step S2 is 30 ℃ and the time is 1-2h.
The working principle and the beneficial effects of the invention are as follows:
1. According to the invention, the pistacia chinensis bunge extract is obtained through leaching of two solutions, the first leaching solution is used for leaching the large-polarity flavonoid compounds in pistacia chinensis bunge stems and leaves, the second leaching solution is used for leaching the fat-soluble flavonoid compounds in pistacia chinensis bunge stems and leaves, and the two leaching solutions act together, so that the extraction rate of pistacia chinensis bunge extract is improved, and meanwhile, the content of flavonoid substances in the extract is also improved.
2. According to the invention, the pistacia chinensis bunge stem and leaf is extracted by using a methanol solution, and then is extracted by using isopropyl acetate and/or Ding Tongjin, so that not only is the extraction rate of pistacia chinensis bunge extract improved, but also the leaching of non-flavonoid substances in pistacia chinensis bunge extract is reduced, and the content of flavonoid substances in pistacia chinensis bunge extract is improved.
3. The temperature of each step is controlled within 70 ℃, so that the damage to flavonoid substances is avoided greatly, and the content of flavonoid substances in the pistacia chinensis bunge extract is improved.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
S1: 1000g of pistacia chinensis bunge stem leaves are weighed, washed by tap water, naturally dried, crushed into pistacia chinensis bunge stem leaf powder with the particle size of 0.5-1cm by a crusher, 10g of crushed materials are weighed into a reaction kettle, 1000mL of 40% methanol solution with the volume fraction is added, the temperature is controlled at 35 ℃, stirring is stopped for 5 hours, stirring is stopped, and the first filtrate and the first filter residue are obtained by filtering.
S2: mixing the first filter residue with 1000mL of isopropyl acetate and butanone with the volume ratio of 2:5, controlling the temperature to 30 ℃, and carrying out countercurrent leaching for 1h to obtain a second filtrate and a second filter residue.
S3: mixing the first filtrate with the second filtrate, distilling at 60deg.C under reduced pressure, stopping distilling when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
Example 2
S1: 10g of pistacia chinensis bunge stem leaf powder in example 1 is weighed into a reaction kettle, 3000mL of 30% methanol solution with volume fraction is added, the temperature is controlled at 45 ℃, stirring is carried out for 4 hours, stirring is stopped, and filtering is carried out, so as to obtain a first filtrate and a first filter residue.
S2: mixing the first filter residue with 1000mL of isopropyl acetate and butanone with the volume ratio of 2:5, controlling the temperature to 30 ℃, and carrying out countercurrent leaching for 1h to obtain a second filtrate and a second filter residue.
S3: mixing the first filtrate with the second filtrate, distilling at 60deg.C under reduced pressure, stopping distilling when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
Example 3
S1: 10g of pistacia chinensis bunge stem leaf powder in example 1 is weighed into a reaction kettle, 2000mL of 35% methanol solution with volume fraction is added, the temperature is controlled at 40 ℃, stirring is carried out for 4.5 hours, stirring is stopped, and filtering is carried out, so as to obtain a first filtrate and a first filter residue.
S2: mixing the first filter residue with 1000mL of isopropyl acetate and butanone with the volume ratio of 2:5, controlling the temperature to 30 ℃, and carrying out countercurrent leaching for 1h to obtain a second filtrate and a second filter residue.
S3: mixing the first filtrate with the second filtrate, distilling at 60deg.C under reduced pressure, stopping distilling when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
Example 4
S1: 10g of pistacia chinensis bunge stem leaf powder in example 1 is weighed into a reaction kettle, 1000mL of 40% methanol solution with volume fraction is added, the temperature is controlled at 35 ℃, stirring is carried out for 5 hours, stirring is stopped, and filtering is carried out, so as to obtain a first filtrate and a first filter residue.
S2: mixing the first filter residue with 500mL of isopropyl acetate and butanone with the volume ratio of 2:5, controlling the temperature to 30 ℃, and carrying out countercurrent leaching for 2 hours to obtain a second filtrate and a second filter residue.
S3: mixing the first filtrate with the second filtrate, distilling at 60deg.C under reduced pressure, stopping distilling when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
Example 5
S1: 10g of pistacia chinensis bunge stem leaf powder in example 1 is weighed into a reaction kettle, 1000mL of 40% methanol solution with volume fraction is added, the temperature is controlled at 35 ℃, stirring is carried out for 5 hours, stirring is stopped, and filtering is carried out, so as to obtain a first filtrate and a first filter residue.
S2: mixing the first filter residue with 1000mL of isopropyl acetate and butanone with the volume ratio of 1:1, controlling the temperature to 30 ℃, and carrying out countercurrent leaching for 1h to obtain a second filtrate and a second filter residue.
S3: mixing the first filtrate with the second filtrate, distilling at 60deg.C under reduced pressure, stopping distilling when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
Example 6
S1: 10g of pistacia chinensis bunge stem leaf powder in example 1 is weighed into a reaction kettle, 1000mL of 40% methanol solution with volume fraction is added, the temperature is controlled at 35 ℃, stirring is carried out for 5 hours, stirring is stopped, and filtering is carried out, so as to obtain a first filtrate and a first filter residue.
S2: mixing the first filter residue with 1000mL of isopropyl acetate and butanone with the volume ratio of 3:5, controlling the temperature to 30 ℃, and carrying out countercurrent leaching for 1h to obtain a second filtrate and a second filter residue.
S3: mixing the first filtrate with the second filtrate, distilling at 60deg.C under reduced pressure, stopping distilling when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
Example 7
S1: 10g of pistacia chinensis bunge stem leaf powder in example 1 is weighed in a reaction kettle, 1000mL of 40% methanol solution with volume fraction is added, the temperature is controlled at 35 ℃, stirring is carried out for 5 hours, stirring is stopped, and filtering is carried out, so as to obtain a first filtrate and a first filter residue.
S2: mixing the first filter residue with 1000mL of isopropyl acetate and butanone with the volume ratio of 4:5, controlling the temperature to 30 ℃, and carrying out countercurrent leaching for 1h to obtain a second filtrate and a second filter residue.
S3: mixing the first filtrate with the second filtrate, distilling at 60deg.C under reduced pressure, stopping distilling when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
Example 8
S1: 10g of pistacia chinensis bunge stem leaf powder in example 1 is weighed into a reaction kettle, 1000mL of 40% methanol solution with volume fraction is added, the temperature is controlled at 35 ℃, stirring is carried out for 5 hours, stirring is stopped, and filtering is carried out, so as to obtain a first filtrate and a first filter residue.
S2: and (3) carrying out countercurrent leaching on the first filter residue and 1000mL of isopropyl acetate mixed solution at the temperature of 30 ℃ for 1h to obtain a second filtrate and a second filter residue.
S3: mixing the first filtrate with the second filtrate, distilling at 60deg.C under reduced pressure, stopping distilling when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
Example 9
S1: 10g of pistacia chinensis bunge stem leaf powder in example 1 is weighed into a reaction kettle, 1000mL of 40% methanol solution with volume fraction is added, the temperature is controlled at 35 ℃, stirring is carried out for 5 hours, stirring is stopped, and filtering is carried out, so as to obtain a first filtrate and a first filter residue.
S2: and (3) mixing the first filter residue with 1000mL of butanone, controlling the temperature to be 30 ℃, and carrying out countercurrent leaching for 1h to obtain a second filtrate and a second filter residue.
S3: mixing the first filtrate with the second filtrate, distilling at 60deg.C under reduced pressure, stopping distilling when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
Comparative example 1
The difference from example 1 is that the first leaching solution used is a 40% volume fraction ethanol solution.
Comparative example 2
The difference from example 1 is that the second leaching solution used is a mixed solution of isopropyl acetate and acetone in a volume ratio of 2:5.
Comparative example 3
The difference from example 1 is that the second leaching solution used is a mixed solution of ethyl acetate and butanone in a volume ratio of 2:5.
Comparative example 4
S1: 10g of pistacia chinensis bunge stem leaf powder in example 1 is weighed into a reaction kettle, 1000mL of 40% methanol solution with volume fraction is added, the temperature is controlled at 35 ℃, stirring is carried out for 5 hours, stirring is stopped, and filtering is carried out, so that a filter cake and filter residues are obtained.
S2: distilling the filtrate obtained in step S1 under reduced pressure at 60deg.C, stopping distillation when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
Comparative example 5
S1: weighing mixed solution of 10g of pistacia chinensis bunge stem and leaf powder and 1000mL of isopropyl acetate and butanone with volume ratio of 2:5 in example 1, controlling the temperature to 30 ℃, and carrying out countercurrent leaching for 1h to obtain filtrate and filter residue.
S2: and (3) placing the filter cake obtained in the step (S1) into a reaction kettle, adding 1000mL of 40% methanol solution by volume fraction, controlling the temperature to 35 ℃, stirring for 5 hours, stopping stirring, and filtering to obtain a filter cake and filter residues.
S3: mixing the first filtrate with the second filtrate, distilling at 60deg.C under reduced pressure, stopping distilling when about half of the filtrate is distilled off, and lyophilizing to obtain Pistacia chinensis extract.
The pistacia chinensis bunge extracts obtained in the preparation methods of examples 1-9 and comparative examples 1-5 were weighed, and the extraction rate was calculated according to the following formula:
Extraction yield = (pistacia chinensis bunge extract weight/10) ×100%
Rutin is used as a reference substance, a standard solution is prepared, and the content of flavonoid substances (total flavonoids) in pistacia chinensis bunge extracts obtained in examples 1-9 and comparative examples 1-5 is measured, and the measurement results are shown in the following table:
TABLE 1 extraction yield of Pistacia chinensis extract and total flavone content in extract of examples 1-9 and comparative examples 1-5
As can be seen from the data in Table 1, the leaching methods of examples 1 to 9 not only increased the extraction rate of Pistacia chinensis extract, but also increased the content of flavonoids in Pistacia chinensis extract, as compared with the leaching methods of comparative examples 1 to 5.
Compared with example 1, the extract yield of pistacia chinensis bunge in examples 8 and 9 and the flavonoid content in pistacia chinensis bunge extract are relatively low, because the second leaching solution is only isopropyl acetate or only butanone when pistacia chinensis bunge extract is extracted in examples 8 and 9, which means that the extract yield of pistacia chinensis bunge and the flavonoid content in pistacia chinensis bunge extract are further improved when the second leaching solution is a mixed solution of isopropyl acetate and butanone.
Compared with example 1, the extraction rate of the pistacia chinensis bunge extract and the content of flavonoid substances in the pistacia chinensis bunge extract in comparative examples 1-3 are reduced, and the extraction rate of the pistacia chinensis bunge extract and the content of flavonoid substances in the extract cannot be improved by changing the first leaching solution or the second leaching solution, which shows that the extraction rate of the pistacia chinensis bunge extract and the content of flavonoid substances in the pistacia chinensis bunge extract can be improved only when the first leaching solution is a methanol solution and the second leaching solution is a mixed solution of isopropyl acetate and butanone.
Compared with example 1, the extraction rate of the pistacia chinensis bunge extract and the flavonoid content in the pistacia chinensis bunge extract in comparative examples 4-5 are obviously reduced, and the extraction rate of the pistacia chinensis bunge extract and the flavonoid content in the pistacia chinensis bunge extract cannot be improved by reducing the leaching of the second leaching liquor or exchanging the leaching of the first leaching liquor and the leaching of the second leaching liquor, which indicates that the extraction rate of the pistacia chinensis bunge extract and the flavonoid content in the pistacia chinensis bunge extract can be improved only by adopting the leaching of the first leaching liquor and the leaching of the second leaching liquor.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (2)
1. A method for extracting flavonoid substances from pistacia chinensis bunge, which is characterized by comprising the following steps:
S1: mixing pistacia chinensis bunge stem and leaf powder with a first leaching solution, heating and leaching, and filtering to obtain a first filtrate and first filter residues, wherein the first leaching solution is methanol solution; the volume fraction of the methanol solution is 30-40%;
s2: mixing the first filter residue with a second leaching solution, and carrying out countercurrent leaching to obtain a second filtrate and the second filter residue, wherein the second leaching solution is isopropyl acetate or butanone or a mixed solution of isopropyl acetate and butanone in a volume ratio of 2-5:5;
s3: mixing the first filtrate and the second filtrate, distilling under reduced pressure, and lyophilizing to obtain Pistacia chinensis extract;
The feed liquid ratio of the pistacia chinensis bunge stem leaf powder to the first leaching solution is 1:100-300g/mL;
The heating and leaching temperature is 35-45 ℃ and the time is 4-5h in the step S1;
the feed liquid ratio of the pistacia chinensis bunge stem leaf powder to the second leaching solution is 1:50-100g/mL;
and step S2, countercurrent leaching is carried out at the temperature of 30 ℃ for 1-2h.
2. The method for extracting flavonoid substances from pistacia chinensis bunge according to claim 1, wherein the pistacia chinensis bunge stem and leaf powder is obtained by the following steps:
washing, drying and crushing pistacia chinensis bunge stems and leaves to obtain pistacia chinensis bunge stem and leaf powder, wherein the particle size of pistacia chinensis bunge stem and leaf powder is 0.5-1cm.
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| CN103976066A (en) * | 2014-05-15 | 2014-08-13 | 中国科学院合肥物质科学研究院 | Processing method of pistacia chinensis bunge green tea |
| KR20180105321A (en) * | 2017-03-15 | 2018-09-28 | 한국생명공학연구원 | A use of Pistacia weinmannifolia for skin whitening and wrinkle improvement |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103976066A (en) * | 2014-05-15 | 2014-08-13 | 中国科学院合肥物质科学研究院 | Processing method of pistacia chinensis bunge green tea |
| KR20180105321A (en) * | 2017-03-15 | 2018-09-28 | 한국생명공학연구원 | A use of Pistacia weinmannifolia for skin whitening and wrinkle improvement |
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| Title |
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| 黄连木叶提取物抗氧化活性及相关毒性、抗毒性研究;张旋;汤明礼;邓冠军;陈连运;吴丽芳;;天然产物研究与开发;20130815;第25卷(第08期);1106-1111 * |
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