CN1920000A - Producing technique of Jiuzhou caterpillar fungus containing rich chromium and culture medium - Google Patents
Producing technique of Jiuzhou caterpillar fungus containing rich chromium and culture medium Download PDFInfo
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- CN1920000A CN1920000A CN 200610068744 CN200610068744A CN1920000A CN 1920000 A CN1920000 A CN 1920000A CN 200610068744 CN200610068744 CN 200610068744 CN 200610068744 A CN200610068744 A CN 200610068744A CN 1920000 A CN1920000 A CN 1920000A
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Abstract
The invention discloses the chromic Cordyceps sinensis production technology and culture medium. The technology comprises the following steps: making culture medium, sterilizing, inoculating, culturing, separating and disintegrating. The culture medium comprises potato 80-120g, soya bean cake 40-60g, wheat bran 40-60g, addex-magnesium 8-15g, VB1 0.3-0.6g, amylaceum 10-20g, protease leather 3-7g, monobasic potassium phosphate 1-3g and distilled water 1000g. The other formulation is potato 180-220g, amylaceum 10-30g, protease leather 3-7g, monobasic potassium phosphate 1-3g and distilled water 1000-1200g. The technology has the advantages of simple technology and high chromium content.
Description
Technical field
The present invention relates to a kind of Cordyceps kyushuensis rich in chromium production method and special culture media thereof.
Background technology
Trivalent chromium is the trace element of needed by human, has vital role in regulating carbohydrate metabolism and lipid metabolism.There are two kinds of inorganic chromium and organic chromium in the chromium source that human body obtains, but human body is very low to the specific absorption of inorganic chromium, has only about 1%, and can reach 10%～25% to organic chrome uptake, therefore inorganic chromium being transformed into the organic chromium that easily is absorbed by the body and utilizes, is the problem that people study always.Show that through a large amount of experiments fungi have very strong accumulation ability to chromium unit, and can effectively change inorganic chromium into organic chromium.
Kyushu aweto is to parasitize a kind of Cordyceps on the killing bean hawkmoth larva body, and calendar year 2001, the domestication cultivated success.It has relieving cough and reducing sputum, strengthening by means of tonics, function such as anti-oxidant, anti-ageing, antitumor, the tracheobronchitis that is used for the treatment of among the people, illness such as lung tumors.Domestication's kyushu aweto has that mycelia is dense, growth fast, strong stress resistance, artificial culture easily successfully wait characteristics.Detect by analysis, wild kyushu aweto contains 8 kinds of nucleosides materials, and the content of cordycepin and Cordyceps polysaccharide all is higher than Cordyceps sinensis, and the content of the above-mentioned main medicinal ingredients of kyushu aweto mycelium of artificial culture is higher than wild strain.
Show that through shaker test repeatedly the rich chromium ability of kyushu aweto is particularly outstanding in fungi, but about the product and the not corresponding research report of preparation technology thereof of Cordyceps kyushuensis rich in chromium.
Summary of the invention
For solving the problem of above existence, the medicinal ingredients that makes kyushu aweto with have trivalent chromium hypoglycemic, reducing blood lipid and combine, obtain drug effect and be worth better product innovation, the invention provides a kind of production technique and Cordyceps kyushuensis rich in chromium special culture media of Cordyceps kyushuensis rich in chromium.
The concrete technical scheme of the present invention is:
The culture medium prescription of this Cordyceps kyushuensis rich in chromium is made up of the raw material of following weight ratio: potato 80-120g, soya-bean cake 40-60g, wheat bran 40-60g, sal epsom 8-15g, VB
10.3-0.6g, glucose 10-20g, peptone 3-7g, potassium primary phosphate 1-3g, distilled water 1000g.Also can form: potato 180-220g, glucose 10-30g, peptone 3-7g, potassium primary phosphate 1-3g, distilled water 1000-1200g by the raw material of following weight ratio.
This producing technique of Jiuzhou caterpillar fungus containing rich chromium comprises technologies such as rich chromium medium preparation, sterilization, inoculation, cultivation, separation, pulverizing, and concrete processing step is:
(1) rich chromium medium preparation
According to the culture medium prescription proportioning of above-mentioned Cordyceps kyushuensis rich in chromium, add the chromium trichloride solution for preparing by finite concentration, the concentration of regulating chromium ion in the substratum is regulated pH value between 5-7 between 150-350ppm;
(2) packing, sterilization
The substratum for preparing is sub-packed in the 250ml Erlenmeyer flask, and every bottled substratum 100ml seals the back and sterilized 18-25 minute under 120-130 ℃ of condition;
(3) cooling, inoculation
The sterilization taking-up substratum that finishes naturally cools to below 30 ℃, and under aseptic condition, every bottle graft is gone into the cultured kyushu aweto bacterial classification of 10ml;
(4) cultivate
The culturing bottle that inserts the kyushu aweto bacterial classification is placed in the constant temperature shaking table, and attemperation 23-26 ℃, rotating speed 120-180r/min cultivated 50-70 hour;
(5) separate rich chromium mycelium
The rich chromium mycelium of kyushu aweto after cultivating is poured in the Centrifuge Cup, under the condition of 5000r/min centrifugal 15-20 minute, removed supernatant liquor.
(6) clean
In centrifugal Centrifuge Cup after removing supernatant liquor, add an amount of distilled water and fully stir, with centrifugal under step (5) the same terms, abandon supernatant liquor, three times so repeatedly;
(7) oven dry, pulverizing
Take out cleaning the Cordyceps kyushuensis rich in chromium that obtains after centrifugal, place in the constant temperature baking box, under 55-70 ℃ of condition, dry, promptly get the Cordyceps kyushuensis rich in chromium product after the oven dry back is ground into 60-100 purpose fine powder with pulverizer.
This richness chromium culture medium raw material be easy to get and cost low, cultivate kyushu aweto mycelium productive rate height, the cycle is short, chromium trichloride (CrCl is adopted in the chromium source
3), it is cheap, and stable in properties is soluble in water, and it is convenient to use.
This producing technique of Jiuzhou caterpillar fungus containing rich chromium is simple and practical, and Fu Ge measures high.Adopting the rich chromium product of the kyushu aweto biomass of this prepared is 0.5-1.5g/100ml, and chromium ion concentration reaches 500-700ppm in the mycelium, has improved 3000-5000 doubly than the content in the natural kyushu aweto.
Embodiment
The present invention will be further described below in conjunction with embodiment.
Embodiment one:
The culture medium prescription of this Cordyceps kyushuensis rich in chromium is made up of the raw material of following weight ratio: potato 100g, soya-bean cake 50g, wheat bran 50g, sal epsom 10g, VB
10.5g, glucose 15g, peptone 5g, potassium primary phosphate 2g, distilled water 1000g.
This producing technique of Jiuzhou caterpillar fungus containing rich chromium step is:
(1) rich chromium medium preparation
According to the culture medium prescription proportioning of above-mentioned Cordyceps kyushuensis rich in chromium, add the chromium trichloride solution for preparing by finite concentration, the concentration of regulating chromium ion in the substratum is 250ppm, regulating pH value is 6;
(2) packing, sterilization
The substratum for preparing is sub-packed in the 250ml Erlenmeyer flask, and every bottled substratum 100ml seals the back and sterilized 20 minutes under 127 ℃ of conditions;
(3) cooling, inoculation
The sterilization taking-up substratum that finishes naturally cools to below 30 ℃, and under aseptic condition, every bottle graft is gone into the cultured kyushu aweto bacterial classification of 10ml;
(4) cultivate
The culturing bottle that inserts the kyushu aweto bacterial classification is placed in the constant temperature shaking table, 25 ℃ of attemperation, rotating speed 140r/min cultivated 50 hours;
(5) separate rich chromium mycelium
The rich chromium mycelium of kyushu aweto after cultivating is poured in the Centrifuge Cup, under the condition of 5000r/min centrifugal 20 minutes, removed supernatant liquor.
(6) clean
In centrifugal Centrifuge Cup after removing supernatant liquor, add an amount of distilled water and fully stir, with centrifugal under step (5) the same terms, abandon supernatant liquor, three times so repeatedly;
(7) oven dry, pulverizing
Take out cleaning the Cordyceps kyushuensis rich in chromium that obtains after centrifugal, place in the constant temperature baking box, under 60 ℃ of conditions, dry, promptly get the Cordyceps kyushuensis rich in chromium product after the oven dry back is ground into 80 purpose fine powders with pulverizer.
Embodiment two:
The culture medium prescription of this Cordyceps kyushuensis rich in chromium is made up of the raw material of following weight ratio: potato 200g, glucose 20g, peptone 5g, potassium primary phosphate 2g, distilled water 1000g.
This producing technique of Jiuzhou caterpillar fungus containing rich chromium step is:
(1) rich chromium medium preparation
According to the culture medium prescription proportioning of above-mentioned Cordyceps kyushuensis rich in chromium, add the chromium trichloride solution for preparing by finite concentration, the concentration of regulating chromium ion in the substratum is 250ppm, regulating pH value is 6;
(2) packing, sterilization
The substratum for preparing is sub-packed in the 250ml Erlenmeyer flask, and every bottled substratum 100ml seals the back and sterilized 20 minutes under 127 ℃ of conditions;
(3) cooling, inoculation
The sterilization taking-up substratum that finishes naturally cools to below 30 ℃, and under aseptic condition, every bottle graft is gone into the cultured kyushu aweto bacterial classification of 10ml;
(4) cultivate
The culturing bottle that inserts the kyushu aweto bacterial classification is placed in the constant temperature shaking table, 25 ℃ of attemperation, rotating speed 140r/min cultivated 50 hours;
(5) separate rich chromium mycelium
The rich chromium mycelium of kyushu aweto after cultivating is poured in the Centrifuge Cup, under the condition of 5000r/min centrifugal 20 minutes, removed supernatant liquor.
(6) clean
In centrifugal Centrifuge Cup after removing supernatant liquor, add an amount of distilled water and fully stir, with centrifugal under step (5) the same terms, abandon supernatant liquor, three times so repeatedly;
(7) oven dry, pulverizing
Take out cleaning the Cordyceps kyushuensis rich in chromium that obtains after centrifugal, place in the constant temperature baking box, under 60 ℃ of conditions, dry, promptly get the Cordyceps kyushuensis rich in chromium product after the oven dry back is ground into 80 purpose fine powders with pulverizer.
The Cordyceps kyushuensis rich in chromium product that above technology is produced makes an addition in food or the salt according to different demands, or is prepared into product such as capsule, needs the requirement of benefit chromium to satisfy people because of lacking chromium.
Claims (5)
1, a kind of substratum of Cordyceps kyushuensis rich in chromium, it is characterized in that its prescription is made up of the raw material of following weight ratio: potato 80-120g, soya-bean cake 40-60g, wheat bran 40-60g, sal epsom 8-15g, VB10.3-0.6g, glucose 10-20g, peptone 3-7g, potassium primary phosphate 1-3g, distilled water 1000-1200g.
2, the substratum of Cordyceps kyushuensis rich in chromium according to claim 1 is characterized in that its prescription is made up of the raw material of following weight ratio: potato 100g, soya-bean cake 50g, wheat bran 50g, sal epsom 10g, VB10.5g, glucose 15g, peptone 5g, potassium primary phosphate 2g, distilled water 1000g.
3, a kind of substratum of Cordyceps kyushuensis rich in chromium is characterized in that its prescription is made up of the raw material of following weight ratio: potato 180-220g, glucose 10-30g, peptone 3-7g, potassium primary phosphate 1-3g, distilled water 1000-1200g.
4, the substratum of Cordyceps kyushuensis rich in chromium according to claim 3 is characterized in that its prescription is made up of the raw material of following weight ratio: potato 200g, glucose 20g, peptone 5g, potassium primary phosphate 2g, distilled water 1000g.
5, a kind of producing technique of Jiuzhou caterpillar fungus containing rich chromium is characterized in that comprising technologies such as rich chromium medium preparation, sterilization, inoculation, cultivation, separation, pulverizing, and concrete processing step is:
(1) rich chromium medium preparation
According to the culture medium prescription proportioning of claim 1 or 3 described Cordyceps kyushuensis rich in chromiums, add the chromium trichloride solution for preparing by finite concentration, the concentration of regulating chromium ion in the substratum is regulated pH value between 5-7 between 150-350ppm;
(2) packing, sterilization
The substratum for preparing is sub-packed in the 250ml Erlenmeyer flask, and every bottled substratum 100ml seals the back and sterilized 18-25 minute under 120-130 ℃ of condition;
(3) cooling, inoculation
The sterilization taking-up substratum that finishes naturally cools to below 30 ℃, and under aseptic condition, every bottle graft is gone into the cultured kyushu aweto bacterial classification of 10ml;
(4) cultivate
The culturing bottle that inserts the kyushu aweto bacterial classification is placed in the constant temperature shaking table, and attemperation 23-26 ℃, rotating speed 120-180r/min cultivated 50-70 hour;
(5) separate rich chromium mycelium
The rich chromium mycelium of kyushu aweto after cultivating is poured in the Centrifuge Cup, under the condition of 5000r/min centrifugal 15-20 minute, removed supernatant liquor.
(6) clean
In centrifugal Centrifuge Cup after removing supernatant liquor, add an amount of distilled water and fully stir, with centrifugal under step (5) the same terms, abandon supernatant liquor, three times so repeatedly;
(7) oven dry, pulverizing
Take out cleaning the Cordyceps kyushuensis rich in chromium that obtains after centrifugal, place in the constant temperature baking box, under 55-70 ℃ of condition, dry, promptly get the Cordyceps kyushuensis rich in chromium product after the oven dry back is ground into 60-100 purpose fine powder with pulverizer.
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| CNB2006100687442A CN100497587C (en) | 2006-09-04 | 2006-09-04 | Producing technique of Jiuzhou caterpillar fungus containing rich chromium and culture medium |
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| CNB2006100687442A CN100497587C (en) | 2006-09-04 | 2006-09-04 | Producing technique of Jiuzhou caterpillar fungus containing rich chromium and culture medium |
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| CN1920000A true CN1920000A (en) | 2007-02-28 |
| CN100497587C CN100497587C (en) | 2009-06-10 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102037852A (en) * | 2009-10-12 | 2011-05-04 | 吴江市家和蚕业专业合作社 | Sterile worm grass culturing chamber |
| CN102428832A (en) * | 2011-09-14 | 2012-05-02 | 大连百祥聚生物科技有限公司 | Fungal pharmaceutical mycoplasm with blood sugar lowering efficacy and preparation method thereof |
| CN101659578B (en) * | 2009-09-18 | 2012-10-17 | 鲁东大学 | Artificial culture method of Kyushu cordyceps sinensis and culture medium thereof |
| CN103626304A (en) * | 2013-12-09 | 2014-03-12 | 湖北工业大学 | Application of cordyceps sinensis fermentation mycelium in heavy metal wastewater treatment, and treatment method |
-
2006
- 2006-09-04 CN CNB2006100687442A patent/CN100497587C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101659578B (en) * | 2009-09-18 | 2012-10-17 | 鲁东大学 | Artificial culture method of Kyushu cordyceps sinensis and culture medium thereof |
| CN102037852A (en) * | 2009-10-12 | 2011-05-04 | 吴江市家和蚕业专业合作社 | Sterile worm grass culturing chamber |
| CN102428832A (en) * | 2011-09-14 | 2012-05-02 | 大连百祥聚生物科技有限公司 | Fungal pharmaceutical mycoplasm with blood sugar lowering efficacy and preparation method thereof |
| CN102428832B (en) * | 2011-09-14 | 2013-04-03 | 大连百祥聚生物科技有限公司 | Fungal pharmaceutical mycoplasm with blood sugar lowering efficacy and preparation method thereof |
| CN103626304A (en) * | 2013-12-09 | 2014-03-12 | 湖北工业大学 | Application of cordyceps sinensis fermentation mycelium in heavy metal wastewater treatment, and treatment method |
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| Publication number | Publication date |
|---|---|
| CN100497587C (en) | 2009-06-10 |
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Granted publication date: 20090610 Termination date: 20120904 |