CN1189488A - Method for preparing cystine coproduced non-salt compound amino-acid - Google Patents

Method for preparing cystine coproduced non-salt compound amino-acid Download PDF

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Publication number
CN1189488A
CN1189488A CN97101347A CN97101347A CN1189488A CN 1189488 A CN1189488 A CN 1189488A CN 97101347 A CN97101347 A CN 97101347A CN 97101347 A CN97101347 A CN 97101347A CN 1189488 A CN1189488 A CN 1189488A
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China
Prior art keywords
acid
preparation
salt compound
compound amino
cystine
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CN97101347A
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Chinese (zh)
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CN1055286C (en
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吴芝清
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Hebei University
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Hebei University
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Priority to CN97101347A priority Critical patent/CN1055286C/en
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Publication of CN1055286C publication Critical patent/CN1055286C/en
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  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

A process for preparing both cystine and non-salt composite amino acids as by-product includes hydrolysis of raw protein material, decolouring, suction filter, electric dialysis to reduce acidity, neutralization deposition, suction filter, and desalting the filtrate to obtain non-salt composite amino acids, and features high output rate of cystine and use of the composite amino acids in hydrolyzed liquid.

Description

The preparation method of cystine coproduced non-salt compound amino-acid
The present invention relates to a kind of preparation method of cystine coproduced non-salt compound amino-acid.
Gelucystine (L-cystine, cyss) the scleroprotein hydrolysis method is generally adopted in production, be precipitation Gelucystine during with alkali neutralizing hydrolysis liquid, must generate a large amount of salt (NaCl), it is the important factor that causes product yield low that a large amount of salt can't be handled, and also makes the very difficult development and use of ten multiple amino acids except that Gelucystine in the hydrolyzed solution, and a large amount of neutralizers are as discharging of waste liquid, both wasted valuable protein resource, more caused environmental pollution.
The preparation method who the object of the present invention is to provide a kind of cystine coproduced non-salt compound amino-acid is to overcome the deficiency that prior art exists.
Integrated artistic step of the present invention is:
The preparation method of cystine coproduced non-salt compound amino-acid comprises the protein raw material acid hydrolysis, and activated carbon decolorizing is with alkali neutralizing hydrolysis liquid; To behind suction filtration, do to fall acid treatment through the hydrolyzed solution behind the activated carbon decolorizing with electrodialyzer, with in the alkali lye and above-mentioned feed liquid muddiness and PH greater than 3, after will above-mentioned feed liquid filtering the Gelucystine precipitation, filtrate is made non-salt compound amino-acid via desalination.
Each process steps is among the present invention:
Add acid in the protein raw material and be technical hydrochloric acid, the two weight ratio is 1: 1.5-2.
Service temperature is 70 ℃-100 ℃ in the activated carbon decolorizing process, and churning time is 1-2 hour.
First dilute with water feed liquid to acidity is lower than 1.5 equivalents before the hydrolyzed solution after the decolouring is done to fall acid treatment.
Filtering filtrate after the neutralization adopts electrodialyzer to carry out desalination.
The invention will be further described below in conjunction with embodiment:
The weight ratio of protein raw material and technical hydrochloric acid is 1: 1.8 in the present embodiment, and activated carbon dosage is 0.7-1.0/0.D495m 3Operational condition is 85 ℃, stirred 1.5 hours, the removal of solid substance is finished by the board and frame machine negative pressure leaching in waste active carbon and the hydrolyzed solution, filter cake is with 0.3 times of hot wash of hydrolyzed solution volume, washings and filtrate merge, falling acid through the filtrate behind Plate Filtration dilution makes to fall acid treatment suction filtration to the reaction end to 1.5 equivalents filtrate is warming up to 45 ℃ with electrodialyzer, slowly stir and drip 2N-3N NaOH solution, treat to stop to add alkali after the solution becomes muddiness, cultivated nucleus 20 minutes, with the Gelucystine crude product that makes through secondary crystal, behind three grades of crystal refinings the Gelucystine finished product, with the filtrate behind the suction filtration with ortho-water handle with electrodialyzer do in short-term with electrodialysis fall and promptly get non-salt compound amino-acid liquid after salt is handled, above-mentioned feed liquid is through sparging the dry pulvis that promptly gets.
The substantive distinguishing features that the present invention is obtained and remarkable technological progress are that this preparation method not only can make Guang ammonia The yield of acid significantly improves, and also makes to extract behind the cystine salinity in the neutralizer and be down to extremely low, this salt-free Compound amino acid can be widely used in feed, food, cosmetics and pharmaceuticals industry.

Claims (5)

1, the preparation method of cystine coproduced non-salt compound amino-acid comprises the protein raw material acid hydrolysis, and activated carbon decolorizing is with alkali neutralizing hydrolysis liquid; It is characterized in that and will behind suction filtration, do to fall acid treatment through the hydrolyzed solution behind the activated carbon decolorizing with electrodialyzer, with in the alkali lye and above-mentioned feed liquid muddiness and PH greater than 3, after will above-mentioned feed liquid filtering the Gelucystine precipitation, filtrate is made non-salt compound amino-acid via desalination.
2, preparation method according to claim 1 is characterized in that adding in the protein raw material acid and is technical hydrochloric acid, and the two weight ratio is 1: 1.5-2.
3, preparation method according to claim 1 is characterized in that service temperature is 70 ℃-100 ℃ in the activated carbon decolorizing process, and churning time is 1-2 hour.
4, preparation method according to claim 1 is characterized in that first dilute with water feed liquid to acidity is lower than 1.5 equivalents before the hydrolyzed solution after the decolouring is done to fall acid treatment.
5, preparation method according to claim 1, the filtering filtrate after it is characterized in that neutralizing adopts electrodialyzer to carry out desalination.
CN97101347A 1997-01-31 1997-01-31 Method for preparing cystine coproduced non-salt compound amino-acid Expired - Fee Related CN1055286C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN97101347A CN1055286C (en) 1997-01-31 1997-01-31 Method for preparing cystine coproduced non-salt compound amino-acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN97101347A CN1055286C (en) 1997-01-31 1997-01-31 Method for preparing cystine coproduced non-salt compound amino-acid

Publications (2)

Publication Number Publication Date
CN1189488A true CN1189488A (en) 1998-08-05
CN1055286C CN1055286C (en) 2000-08-09

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CN97101347A Expired - Fee Related CN1055286C (en) 1997-01-31 1997-01-31 Method for preparing cystine coproduced non-salt compound amino-acid

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103044297A (en) * 2012-12-07 2013-04-17 重庆工贸职业技术学院 New method for neutralizing and extracting cystine in two steps
CN104313071A (en) * 2014-10-17 2015-01-28 湖南宝利士生物技术有限公司 Biosynthetic method of high purity L-alpha-amino acid
CN110344077A (en) * 2019-07-01 2019-10-18 吉林大学 A method of by l-cysteine electrochemistry formated n-acetyl-L-cysteine
CN111072536A (en) * 2019-12-19 2020-04-28 重庆工贸职业技术学院 Method for simultaneously producing cystine and salt-free compound amino acid

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ213630A (en) * 1984-10-19 1990-02-26 Ici Plc Acrylic acid derivatives and fungicidal compositions
CN1088915A (en) * 1992-12-31 1994-07-06 福建师范大学 Produce the method for L-Gelucystine

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103044297A (en) * 2012-12-07 2013-04-17 重庆工贸职业技术学院 New method for neutralizing and extracting cystine in two steps
CN104313071A (en) * 2014-10-17 2015-01-28 湖南宝利士生物技术有限公司 Biosynthetic method of high purity L-alpha-amino acid
CN110344077A (en) * 2019-07-01 2019-10-18 吉林大学 A method of by l-cysteine electrochemistry formated n-acetyl-L-cysteine
CN111072536A (en) * 2019-12-19 2020-04-28 重庆工贸职业技术学院 Method for simultaneously producing cystine and salt-free compound amino acid

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