CN1073718A - The method of separating thallus from glutami acid fermentation liquor - Google Patents
The method of separating thallus from glutami acid fermentation liquor Download PDFInfo
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- CN1073718A CN1073718A CN 91108274 CN91108274A CN1073718A CN 1073718 A CN1073718 A CN 1073718A CN 91108274 CN91108274 CN 91108274 CN 91108274 A CN91108274 A CN 91108274A CN 1073718 A CN1073718 A CN 1073718A
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Abstract
The method of separating thallus is a kind of separation of industrial fermentation product from glutami acid fermentation liquor, extracting method, particularly with flocculation agent separating thallus from glutami acid fermentation liquor, the processing method of colloid and other solid substance, it is characterized in that adopting flocculation agent chitosan acidic solution, sodium alginate aqueous solution, carboxymethyl cellulose aqueous solution and calcium chloride water mix stirring with glutami acid fermentation liquor, after the flocculation, extract supernatant liquor, stay that heavy-fluid directly pumps into or be pressed into whizzer or pressure filter carries out centrifugal or filter-press dehydration, get the wet thallus convection drying and be ground into unicellular feedstuff protein, the gained supernatant liquor adopts the method for freezing iso-electric point to extract L-glutamic acid, and its yield improves more than 5%.
Description
The present invention is a kind of separation, extracting method of industrial fermentation product, particularly separates, precipitates the processing method of thalline, colloid and other solid substance from glutami acid fermentation liquor with flocculation agent.
The existing method of extracting L-glutamic acid from glutami acid fermentation liquor has isoelectric point method, ion exchange method, hydrochloride method, zincate process, permeable membrane partition method, solvent extraction process etc., wherein carrier concentrated frozen isoelectric point method is at present than advanced person and the higher method of economic benefit, but its main drawback is: contain thalline, protein, colloid and other solid substance in the fermented liquid, disturb and hindered the crystallization of L-glutamic acid, crystalline purity and yield have been influenced, if remove the materials such as thalline in the fermented liquid earlier, use the concentrated frozen isoelectric point method just more advanced again, economic benefit is higher.The existing method of removing the materials such as thalline in the fermented liquid earlier has: use the supercentrifuge mechanical phonograph recorder separation, FESX512S-31C type butterfly chip supercentrifuge as Sweden ALFA-LAVAL company is advanced mechanical separation, but the degerming rate on average has only about 70%, and apparatus expensive, big energy-consuming; The super filtration method of mineral membrane (such as in China Patent No. 85109246 patent documentations introduction) complex process, filtration velocity is slow, mineral membrane price height; Adopt the non-toxic efficient flocculation agent to carry out the method (as introducing in Chinese patent application number 90102179 files) of physical chemistry flocculation, but only mention a kind of flocculation agent chitosan in this application file, use pH value to require to have certain limitation separately to fermented liquid, the effect instability, clear liquid after the flocculation, the ratio of heavy-fluid is little, heavy-fluid (following turbid liquid) volume is too big, therefore, need through sorbent material (as the gac) secondary separation of heating, also to instead transfer more than the pH value to 7.0, the pH value downward modulation direction of extracting L-glutamic acid with next step iso-electric point is opposite, increased the soda acid consumption, influenced the utility value of L-glutamic acid yield and final enriched material, complex process, long flow path, equipment and energy consumption have been increased.Be unfavorable for the utilization of suitability for industrialized production.
The objective of the invention is: provide a kind of from glutami acid fermentation liquor the processing method of separating thallus, protein, colloid and other solid substance, this method technology is fairly simple, flow process is relatively shorter, the thalline velocity of separation than very fast, effect is relatively good.
Technical scheme of the present invention is: the chitosan-containing acidic solution (to call flocculation agent in the following text No. 1) that adds suitable fermented liquid volume 0.1-3% in glutami acid fermentation liquor, 0.5-2.5% is better, after the stirring, at the sodium alginate aqueous solution (to call flocculation agent in the following text No. 2) that continues to add under the condition of stirring suitable fermented liquid volume 0.5-2%, 1-1.5% is better, or add the carboxymethyl cellulose aqueous solution (to call flocculation agent in the following text No. 3) of suitable fermented liquid volume 0.5-2%, 1-1.5% is better, finish and stop to stir, treat that the throw out post precipitation extracts supernatant liquor, stay heavy-fluid (following turbid liquid), No. 1 flocculation agent that under stirring state, adds suitable heavy-fluid volume 0.1%-4%, 1.5-3% is better, No. 2 flocculation agents that add suitable heavy-fluid 1-5% more while stirring, 3-4.5% is better, with the calcium chloride water that is equivalent to heavy-fluid volume 0.5-5% (to call flocculation agent in the following text No. 4), 2.5-3.5% is better, stop to stir after stirring fully, the heavy-fluid that to flocculate directly pumps into or be pressed into whizzer or pressure filter carries out centrifugal or filter-press dehydration, collect clear liquid, get wet thallus convection drying and pulverizing, get unicellular feedstuff protein, merge the supernatant liquor that is extracted, adopt the method for freezing iso-electric point or concentrated frozen iso-electric point to extract L-glutamic acid.
It is more than 160,000 that the chitosan acidic solution adopts molecular weight, amido content greater than 75%, absolute viscosity is greater than 100CP(1% concentration) Powdered chitosan be dissolved in acetate that concentration is 0.5-2% or the formic acid solution and make.
Sodium alginate aqueous solution adopts water-soluble the making of sodium alginate that reaches GB GB1976-80, makes its absolute viscosity greater than 200CP.The absolute viscosity of carboxymethyl cellulose aqueous solution is greater than 400CP.Calcium chloride water is greater than 30 degree Beaume.
Technology of the present invention is simple, velocity of separation is fast, basically do not need to increase new installation and energy consumption just can drop into suitability for industrialized production, thallus removing rate reaches more than 95%, once the ratio of flocculation back clear liquid and heavy-fluid is 8: 2, when best is 9: 1, thalline after the separation can be directly as feedstuff protein, mother liquor GA behind the extraction L-glutamic acid is 1.0-1.2%, and its COD can once drop to about 50000 mg/litre, the no supplementary condition of heavy-fluid dehydration, can carry out mechanical dehydration separates, its hydrophobicity is fine, has solved the key problem in technology that throw out is difficult to dewater, and the total yield of clear liquid reaches 97-98%, even the method that directly adopts freezing iso-electric point to extract without concentrating, its L-glutamic acid yield also can improve more than 5% on the basis that the once freezing iso-electric point of former carrier is extracted, and L-glutamic acid purity can reach about 90%, and transmittance improves 10-20%.If take to concentrate the technology that iso-electric point is extracted after thalline separates; the L-glutamic acid yield can reach more than 90%; especially under the situation of the undesired or fermentor tank microbiological contamination of fermentation (this is normal as long as produce acidic group) adopts present method, still can obtain the L-glutamic acid of higher yields, higher degree.
Embodiment 1: 17500 liters of extracting corn starch fermented liquids, L-glutamic acid content 5.83%, pH value 6.0, RG0.8%, OD0.88, No. 1 flocculation agent of adding is 270 liters under stirring state, after fully stirring, add 140 liters of No. 2 flocculation agents more while stirring, 64 liters of No. 3 flocculation agents finish and stop to stir, and extract supernatant liquor after leaving standstill aggegation, OD0.04, the heavy-fluid of staying adds 70 liters of above-mentioned No. 1 flocculation agents under stirring state, add 140 liters of above-mentioned No. 2 flocculation agents after 3 minutes while stirring, 100 liters of No. 4 flocculation agents of B ' e32, pump into centrifuge dewatering after fully stirring, reclaim clear liquid, get wet thallus water ratio 70.3%, protein 65 .03%, the gained clear liquid, directly adopt freezing iso-electric point to extract L-glutamic acid, wait electric yield 80.22%, bran acid purity 87.63%, transmittance 26%, mother liquor GA1.1%.
Embodiment 2:
20500 liters of extracting corn starch fermented liquids, L-glutamic acid content 5.55%, pH value 6.2, OD0.99, RG0.7%, the COD161600 mg/litre is behind Hcl accent pH value to 5.3, No. 1 flocculation agent of adding is 300 liters under stirring state, add 180 liters of No. 2 flocculation agents after stirring fully while stirring, 60 liters of No. 3 flocculation agents leave standstill aggegation then, treat to extract supernatant liquor after throw out sinks, OD0.045, the COD105600 mg/litre stays heavy-fluid, add 90 liters of above-mentioned No. 1 flocculation agents while stirring, 130 liters of No. 4 flocculation agents that add 180 liters of above-mentioned No. 2 flocculation agents and B ' e32 after 3 minutes under stirring state are after fully stirring, with 20 square metres of plate-and-frame filter press of high-pressure pump injection, filter-press dehydration, reclaim clear liquid, get wet thallus, water ratio 65.04%, protein 67 .12%, directly adopt freezing iso-electric point to extract L-glutamic acid to the gained clear liquid, wait electric yield 81.1%, bran acid purity 89.06%, transmittance 25%, mother liquor GA1.0%.
Embodiment 3: get 20000 liters of corn coarse raw materials direct fermentation liquid, L-glutamic acid content is 6.89%, pH value 6.5, after transferring pH value to 5.2 with Hcl, add 300 liters of No. 1 flocculation agents while stirring, stirs after 3 minutes 250 liters of No. 2 flocculation agents of adding, stop after adding stirring, leave standstill flocculation, after throw out sinks, extract supernatant liquor, OD0.043, the heavy-fluid of staying adds 100 liters of above-mentioned No. 1 flocculation agents under stirring state, add 150 liters of above-mentioned No. 2 flocculation agents after 3 minutes while stirring, 50 liters of No. 3 flocculation agents, 180 liters of No. 4 flocculation agents of B ' e32,20 square metres of plate-and-frame filter press filter-press dehydrations are injected with high-pressure pump in the abundant back of stirring, and reclaim clear liquid, get wet thallus, water ratio 68.48%, albumen 63.96% merges direct freezing iso-electric point with above gained clear liquid and extracts L-glutamic acid, waits electric yield 84%, bran acid purity 91.5%, transmittance 59%, mother liquor GA0.98%, COD52640 mg/litre.
Embodiment four:
15000 liters of extracting corn starch fermented liquids, GA5.59%, RG0.92%, OD1.30PH value 5.2, the COD149648 mg/litre, this jar fermentation was found phage after 8 hours, thalline self-dissolving when putting jar.Add 180 liters of No. 1 flocculation agents, stir and add 120 liters of No. 2 flocculation agents after 5 minutes, stop immediately stirring, extract supernatant liquor after 1 hour, OD0.063, COD104829 mg/litre.Must heavy-fluid stir 120 liters of No. 1 flocculation agents of following adding, add 180 liters of No. 2 flocculation agents after 5 minutes successively, add 150 liters of No. 4 flocculation agents again, continue to stir after 5 minutes and be pressed into 20 square metres of plate-and-frame filter press, 1000 liters of heavy-fluid/20 of speed of filter pressing average out to minute with air compressor machine.Get 500 kilograms of wet thallus, water ratio 65.14% contains protein 65 .42%.Merge the once freezing iso-electric point of gained clear liquid and extract L-glutamic acid.Etc. electric yield 71.84%, purity 86.06%, transmittance 23%, mother liquor GA1.22%, COD57152 mg/litre.
Claims (9)
1, the method for separating thallus from glutami acid fermentation liquor, it is characterized in that: the chitosan-containing acidic solution that in glutami acid fermentation liquor, adds suitable fermented liquid volume 0.1%-3%, after the stirring, at the carboxymethyl cellulose aqueous solution that continues to add the sodium alginate aqueous solution of suitable fermented liquid volume 0.5%-2% under the condition of stirring or add suitable fermented liquid volume 0.5%-2%, finish and stop to stir, treat that the throw out post precipitation extracts supernatant liquor.Stay heavy-fluid (following turbid liquid), the chitosan acidic solution that under stirring state, adds suitable heavy-fluid volume 0.1%-4%, add the sodium alginate aqueous solution of suitable heavy-fluid 1-5% and the calcium chloride water of suitable heavy-fluid volume 0.5-5% more while stirring, the heavy-fluid that to flocculate directly pumps into or be pressed into whizzer or pressure filter carries out centrifugal or filter-press dehydration, collects clear liquid.Get wet thallus convection drying and pulverizing, get unicellular feedstuff protein.Merge the supernatant liquor that is extracted, adopt the method for freezing iso-electric point or concentrated frozen iso-electric point to extract L-glutamic acid.
2, according to claim 1 described from glutami acid fermentation liquor the method for separating thallus, it is characterized in that: the chitosan acidic solution adopts that molecular weight is more than 160,000, amido content greater than 75%, absolute viscosity is greater than 100CP(1% concentration) Powdered chitosan be dissolved in acetate or the formic acid solution.
3, according to claim 2 described from glutami acid fermentation liquor the method for separating thallus, it is characterized in that: the concentration of acetate or formic acid is 0.5-2%.
4, according to claim 1 or 2 described from glutami acid fermentation liquor the method for separating thallus, it is characterized in that: sodium alginate aqueous solution adopts water-soluble the making of sodium alginate that reaches GB GB1976-80, makes its absolute viscosity greater than 200CP.
5, according to claim 1 or 4 described from glutami acid fermentation liquor the method for separating thallus, it is characterized in that: the absolute viscosity of carboxymethyl cellulose aqueous solution is greater than 400CP.
6, according to claim 1 or 4 described from glutami acid fermentation liquor the method for separating thallus, it is characterized in that: calcium chloride water is greater than 30 degree Beaume.
7, according to claim 1 described from glutami acid fermentation liquor the method for separating thallus, it is characterized in that: in glutami acid fermentation liquor, add suitable fermented liquid volume 0.5-2.5% and contain shell pump saccharic acid solution.
8, according to claim 1 described from glutami acid fermentation liquor the method for separating thallus, it is characterized in that: in glutami acid fermentation liquor, add the sodium alginate aqueous solution of suitable fermented liquid volume 1-1.5% or add the carboxymethyl cellulose aqueous solution of suitable fermented soln volume 1-1.5%.
9, according to claim 1 described from glutami acid fermentation liquor the method for separating thallus, it is characterized in that: the acid dope of chitosan that in glutami acid fermentation liquor, adds suitable heavy-fluid volume 1.5-3%.Add suitable heavy-fluid volumetrical 3-4.5% sodium alginate aqueous solution and suitable heavy-fluid volume 2.5-3.5% calcium chloride water again.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 91108274 CN1073718A (en) | 1991-12-21 | 1991-12-21 | The method of separating thallus from glutami acid fermentation liquor |
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| CN 91108274 CN1073718A (en) | 1991-12-21 | 1991-12-21 | The method of separating thallus from glutami acid fermentation liquor |
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| CN1073718A true CN1073718A (en) | 1993-06-30 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1040658C (en) * | 1993-07-16 | 1998-11-11 | 青岛海洋大学 | Two-stage coagulating process for removing mycelium from ferment liquid of glutaminic acid |
| CN101748066B (en) * | 2009-12-30 | 2012-09-05 | 无锡绿水之源生物科技有限公司 | Mycelium concentration method |
| CN103937711A (en) * | 2014-04-03 | 2014-07-23 | 广东美瑞科海洋生物科技有限公司 | Filter pressing processing method for microorganism biomass industrialized culture |
| CN103937710A (en) * | 2014-04-03 | 2014-07-23 | 广东美瑞科海洋生物科技有限公司 | Large industrial production method of microbial biomass |
| CN108251342A (en) * | 2018-03-29 | 2018-07-06 | 广东省农业科学院动物科学研究所 | The rapid flocculation deposition recovery method of living preparation of lactobacillus in a kind of lactobacillus ferment liquid |
| CN110898783A (en) * | 2019-11-15 | 2020-03-24 | 江苏隆昌化工有限公司 | Preparation method of inorganic layered supramolecular material |
| CN113755179A (en) * | 2021-09-08 | 2021-12-07 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for preparing liquid soil conditioner by using amino acid waste liquid |
| WO2023125831A1 (en) * | 2021-12-31 | 2023-07-06 | 无锡药明生物技术股份有限公司 | Method for collecting microbial bodies from fermentation solution suitable for automatic operation |
-
1991
- 1991-12-21 CN CN 91108274 patent/CN1073718A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1040658C (en) * | 1993-07-16 | 1998-11-11 | 青岛海洋大学 | Two-stage coagulating process for removing mycelium from ferment liquid of glutaminic acid |
| CN101748066B (en) * | 2009-12-30 | 2012-09-05 | 无锡绿水之源生物科技有限公司 | Mycelium concentration method |
| CN103937711A (en) * | 2014-04-03 | 2014-07-23 | 广东美瑞科海洋生物科技有限公司 | Filter pressing processing method for microorganism biomass industrialized culture |
| CN103937710A (en) * | 2014-04-03 | 2014-07-23 | 广东美瑞科海洋生物科技有限公司 | Large industrial production method of microbial biomass |
| CN108251342A (en) * | 2018-03-29 | 2018-07-06 | 广东省农业科学院动物科学研究所 | The rapid flocculation deposition recovery method of living preparation of lactobacillus in a kind of lactobacillus ferment liquid |
| CN110898783A (en) * | 2019-11-15 | 2020-03-24 | 江苏隆昌化工有限公司 | Preparation method of inorganic layered supramolecular material |
| CN113755179A (en) * | 2021-09-08 | 2021-12-07 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for preparing liquid soil conditioner by using amino acid waste liquid |
| WO2023125831A1 (en) * | 2021-12-31 | 2023-07-06 | 无锡药明生物技术股份有限公司 | Method for collecting microbial bodies from fermentation solution suitable for automatic operation |
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