CN1181893C - Membrane for use in guided tissue regeneration - Google Patents

Membrane for use in guided tissue regeneration Download PDF

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Publication number
CN1181893C
CN1181893C CNB988117347A CN98811734A CN1181893C CN 1181893 C CN1181893 C CN 1181893C CN B988117347 A CNB988117347 A CN B988117347A CN 98811734 A CN98811734 A CN 98811734A CN 1181893 C CN1181893 C CN 1181893C
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China
Prior art keywords
thin film
collagen
barrier layer
hypothallus
cartilage
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Expired - Fee Related
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CNB988117347A
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Chinese (zh)
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CN1280509A (en
Inventor
�˵á���˹����ϣ
彼得·盖斯特利希
�ˡ�������Ү
泽德尼克·埃克马耶
I
洛瑟·施洛瑟
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Ed Geistlich Soehne AG fuer Chemische Industrie
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Ed Geistlich Soehne AG fuer Chemische Industrie
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    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus

Abstract

The invention provides a multi-layer membrane comprising a matrix layer predominantly of collagen II and having an open sponge-like texture, and at least one barrier layer having a close, relatively impermeable texture. Such a membrane is particularly suitable for use in guided tissue regeneration, in particular for use in vivo in the reconstruction of bone or cartilage tissue.

Description

The thin film of guided tissue regeneration
The present invention relates to a kind of collagen film implant that is used for guided tissue regeneration, specifically, this implant is used for interior reconstruction of body of bone or cartilaginous tissue.
In tissue regeneration, proved the reconstruction of cartilaginous tissue for a long time, be very difficult as in the cartilage injury, rebuilding.The cartilage injury can occur on any joint, although dangerous maximum on the big joint such as knee joint and ankle joint etc.This damage can be because wound, degenerative state or osteochondritis dissecans cause.The cartilage injury forms arthropathic pathogenic (pathomechanical) factor of main machinery.The inflammatory process that the disengaging of enzyme causes a kind of synovial fluid also then causes the breakage of cartilage wearing and tearing and articular surface.The recent trial method of regeneration articular cartilage comprises self articular chondrocytes (CACs) of implant cultivating in the cartilage defects in vivo.Yet this technology only obtains limited success.
Now more generally acceptedly be, need provide a kind of substrate with cell guide function to carry out tissue reconstruction, cell will and be grown between this fiber along the fiber of this substrate.Recently the someone advocate with CAC be seeded in synthetic and natural can be by in the resorbent substrate.Yet use is carried out the cartilaginous tissue reconstruction based on the substrate of polylactic acid, polyglycolic acid and collagen I or III and is just required external chondrocyte to be loaded on this substrate earlier before implanting.This has just caused the challenge of chondrocyte aseptic culture aspect, i.e. giant cell in implant and the organizational interface and fibroblastic immunologic inflammatory reaction problem.
A kind of substrate implant based on collagen I I has been proposed among the WO-A-96/25961, this implant implantable in the body position and rely on the growth of basis chondrocyte on stromal surface to realize the reconstruction of cartilage.Yet this substrate realizes that the ability that cartilaginous tissue is rebuild fully is limited.
Therefore, a kind of successfully inwardly growth of chondrocyte that can make self need be provided, the substrate implant of cartilage tissue regeneration can be made after therefore implanting.We have found that now, can the cartilage regeneration tissue and finally also rebuild new osseous tissue with a kind of collagen I I substrate, this collagen I I substrate is not only isolated but also isolated with following bone or cartilage defects with connective tissue on every side in vivo.Our foresight tells us ... arrives, use a kind of multiwalled thin film implant to achieve the above object, this implant itself can prevent that any surrounding tissue from unwanted inside growth taking place and enter substrate, functions as described above thereby this implant can surgery be implanted to rejected region.
From an aspect, the invention provides a kind of plural layers, these plural layers comprise a hypothallus that is mainly collagen I I and has open spongiosis, and the barrier layer of one deck with relative impermeability structure of sealing at least.
Thin film of the present invention special advantage in use be this archeocyte can not see through or grow into have sealing, the barrier layer of impermeability structure relatively.
Though be not wishing to be bound by theory, but we believe now, in order cartilage successfully to be regenerated just must not only will stop as the inwardly growth but also will stop any new osseous tissue and inwardly grow into rejected region fast fast of basis histiocytes such as connective tissue, blood vessel.And this can the application of the invention bilayer film realize, this thin film can protect collagen stroma in case the basis histiocyte from the ingrown influence of a side.When surgery is implanted, this thin film can with tissue grafts, for example periosteum graft is united use, prevents that effectively the basis histiocyte from inwardly growing from offside.Therefore, for example, periosteum graft can be sewed up earlier to put in place becomes covering on bone or the cartilage defects.A slice bilayer film of the present invention can be implanted rejected region then contacting with implant, and settle by this way, promptly the hypothallus of bilayer film one side is towards the bone defective.More preferably, earlier bilayer film of the present invention is implanted rejected region, its barrier layer is towards bone or cartilage defects.Then the periosteum implant is placed on the contacted position of hypothallus on.Graft can cling with the biocompatible adhesive as fibrin adhesive ﹠, or pin with absorbable polylactic acid safety pin (pins), or if desired and may, they are united by a seam by this way so that its be used to provide an impervious barrier layer with prevent any around connective tissue inwardly grow.
In another embodiment of the invention, thin film itself can stop effectively that any basis histiocyte inwardly grows.Therefore, on the other hand, the invention provides a kind of trilaminar thin film that comprises at least, hypothallus is wherein mainly made by collagen I I, and have open spongy tissue, and this hypothallus is between two barrier layers, and this barrier layer has sealing, relative impermeability structure.
Hypothallus can be used as makes the ingrown culture medium of basis chondrocyte, thereby makes cartilage tissue regeneration.Yet in order more to help the regeneration of cartilaginous tissue, hypothallus can pour into chondrocyte before or after implanting.Although can be before hypothallus faces implantation, with for example injecting method, the perfusion chondrocyte be expected at the direct afterwards perfusion chondrocyte of implantation suspension chondrocyte is entered in the hypothallus.By this method, be present in the regeneration that chondrocyte in the film matrix layer can be finished cartilage, and the final new bone of regeneration.This thin film also stops the inside growth of other cell type in the surrounding tissue simultaneously.
The used chondrocyte of the present invention can derive from the cell source that comprises allothigene or spontaneous cell, and these cells are from articular cartilage, periosteum and perichondrium, result from the stem cell of mesenchyme (matter) of bone marrow and separate.Because he gives birth to cell and carries potential immunoreation and infect complication, therefore preferred from the particularly spontaneous articular cartilage of spontaneous cell isolation of chondrocytes.The technology of obtaining cell is known, comprises enzymic digestion (enzymatic digestion) or other branch (outgrowth) cultivation.Then the cell of being gathered in the crops was expanded in cell culture before refilling human body.In order to obtain best cartilage tissue regeneration, the cell number of implanting hypothallus is at least 10 usually 6Individual, preferably be at least 10 7Individual.
The general requirement of the hypothallus of thin film of the present invention contained such as glycosaminoglycans (GAGs) such as hyaluronic acid, chrondroitin 6-sulfate, keratin sulfate, dermatan (dermatan) sulfate, and its effect provides the natural medium that a kind of chondrocyte can be imbedded and grow.Although might will incorporate in the collagen stroma from the glycosaminoglycan of separate sources, these glycosaminoglycans there is no need and from the glycosaminoglycan of cartilage same composition, molecular weight and physiological characteristics arranged, but preferred glycosaminoglycan is from cartilage extraction itself and get.Usually, preferably contain 1 to 10% (weight) in the hypothallus, for example the glycosaminoglycan of 2% to 6% (weight).Though may also have some glycosaminoglycan in the impermeable barrier, wherein major part will be present in the hypothallus.
In the basis collagen tissue, have at least a part of GAGs to exist with proteoglycan (PGs) component form.Because the protein component among the PGs can cause potential immunological problem, it is undesirable using the GAGs of PGs form, thus preferably, in the hypothallus basically without any proteoglycan.The mixture that can telomerize peptide (telopeptide-free) collagen I I material and glycosaminoglycan with the nothing of purification prepares hypothallus, just can accomplish this point easily.
Other additive that also may exist in the hypothallus comprises: for example, the help chondrocyte is attached to chondronectin (chondronectin), laminin (laminin), fibronectin, calcium alginate or the anchorin-II on the collagen I I fiber; Somatomedin, the transforming growth factor (TGF β) that exists as cartilage-inducing factor (CIF), insulin like growth factor (IGF), as homodimer or heterodimer; And bone morphogenic factor (BMP), as human BMP-2, BMP-3 (bone growth promoting element), BMP-4 and BMP-7 (OP-1, bone growth promoting protein-1) basis or reorganization.BMP-2 influences osteoplastic two kinds of approach independently: directly form bone and form cartilage earlier and remove then, replaced by bone.The complex of BMPs and collagen induces the noncollagen protein matter (NCP) of chondrigen to be made up of 90% collagen and 10% as BMP active substance or BMP/NCP, and collagen wherein comprises the bone matrix that extracts or the bone matrix of demineraliting from the cortical bone in various sources.Bone matrix-soluble collagen stroma and laminin (laminin) or fibronectin can be used as the carrier of BMP.Also may have some somatomedin in the impermeable barrier, but most of somatomedin is present in the hypothallus.Thin film generally contains 100 micrograms to 5 milligram somatomedin.
As mentioned above, thin film comprises two heteroid layers at least.The barrier layer of thin film is preferably mainly made by collagen I and collagen I II.Perhaps, the barrier layer can be made by synthetic material, for example by a kind of synthetic can being made by resorbent polymer network, optionally is coated with the collagen-based materials as collagen I and/or collagen I II on the network.
The exemplary synthetic material that is suitable for comprises the homopolymer and the copolymer of polyester, polyglycolic acid and polylactic acid (PLA), Acetic acid, hydroxy-, bimol. cyclic ester and lactide copolymer, poe and polycaprolactone.More than to have many in these examples are public offerings, the RESOMER series of Boehringer Ingelheim for example.Preferred PLA polymer is a wax, and suitable molecular size is about 650-1200 and degradation speed is not too fast.A kind of particularly preferred biological degradation polyalcohol is poly-(D, L-lactic acid), and wherein the ratio of D-lactide and L-lactide is about 70: 30.The advantage of this synthetic material is the mechanical stability height, the thin film implant is stretched on the three-dimensional bone defective of complexity and can not tear.This material also is suitable for sewing up.
It is useful that the barrier layer is made by long collagen fiber, connects to such an extent that so tight consequently polymer substance can not penetrate this barrier between the described fiber.Long fiber provides high tensile strength and tearing toughness, so that still not a kind of good isolation thin film of this material but also be convenient to sew up.The very important point is that the thin film implant should be able to be sewed up or follow closely (pinned) on corresponding position in surgical operation, and many thin film that the past proposed do not have this performance.Thin film of the present invention has enough mechanical stabilities and is suitable for the surgery implantation.
Hypothallus is very loose, and its proportion is low to moderate 0.02, so cell can be very fast grows into hypothallus.This one deck that contains glucosaminoglycan of thin film can expand consumingly and absorbs the liquid of as many as 5000%.It is desirable to, hypothallus should have pore structure (pore fraction and hole dimension), and this structure permissive cell attaches and growth, and allows that the cell of being inoculated keeps its phenotype of chondrocytes, is characterized as synthetic cartilage specificity protein.Hole size depends on the freeze-dry process of making collagen I I substrate, but can expect that hole size in 10 to 120 micrometer ranges, for example is 20 to 100 microns, also may be selected to be about in the of 85 microns.By under-5 to-10 ℃ of temperature, slowly carrying out lyophilizing in freezing about 24 hours subsequently, or be easy to obtain such pore size in the method that in slurry, adds ammonium hydrogencarbonate before the lyophilizing.
The hypothallus of thin film is preferably made with the collagen I I material of taking from cartilage, preferably taking from the hyaline cartilage of pig.
Though the thickness of needed hypothallus will be decided according to the character of pending bone or cartilage defects, generally can expect its thickness in 2 to 10 millimeters scopes, for example 4 to 6 millimeters.Preferably 0.2 to 2 millimeter of the thickness on barrier layer, for example 0.2 to 0.7 millimeter.
The barrier layer can adopt the natural animal thin film that contains collagen I and III to make.Owing to be to take from natural source, therefore can absorb fully in vivo, do not form deleterious catabolite.This thin film still is that dry state all has extra high tearing strength at hygrometric state, so if just have and need carry out surgical stapling.This elastic properties of materials is very big under the situation of humidity, therefore can stretch on the bone defective of irregular molding.
Except collagen, natural animal thin film also contains many other biological substances that must remove.Known and availablely handled with purification to this thin film and use it for medicine such as enzyme, solvent or other chemicals.Great majority in this material all are too thin and usually use especially easily.Collagen fiber have lost its original feature; And further shortcoming arranged: but its intensity is often insufficient as a kind of suture material, and do not have the performance of water-swellable, and do not have the branch of slick granule face and cellulosic flesh noodles.The nothing that purifies telomerizes the fiber-based material of the I type of peptide or II Collagen Type VI can biodegradation, and has less solubility, is the maximum carrier material of advantage that has been found that.
Provide the thin film on the barrier layer of product of the present invention to comprise the thin film that keeps its natural collagen structure to take from calf or pig peritoneum.The thin film of taking from pig and be age the young pig peritoneum in 6 to 7 weeks (weight is 60 to 80 kilograms) is particularly preferred.
Purified, natural (unmodified) insoluble collagen is preferably contained on the barrier layer, and can prepare according to the described method of WO-A-95/18638.Therefore natural thin film can at first be used alkali, and for example working concentration is that the aqueous NaOH of 0.2 to 4% (weight) is handled.Acting as of this processing carried out saponification with any fat and alkali-sensitive protein.Second step generally used the mineral acid as HCl that material is handled with acid, and its purpose is for eliminating the pollutant of acid labile.Material is washed until pH value then and reached 2.5 to 3.5 scopes.Like this, thin film just has looser more cellulosic of a slick or graininess face and.This to by be heated to 100 to 120 ℃ realize some of thin film crosslinked be useful.
Can be with coming from cartilage, to obtain to provide the collagen I I of film matrix layer material with the above-mentioned barrier layer similar methods of mainly forming of obtaining by collagen I and III.Cartilage is preferably handled to remove moisture with acetone, use subsequently such as the such hydrocarbon solvent of normal hexane and carry out extracting fat, although also can use such as such chlorohydrocarbon of the such alkanol of ethanol, diethyl ether such ether, chloroform or their mixture.Then the material after the defat is handled with alkali so that any remaining fatty saponification and make some protein degradation of existence.Material will be handled so that protein is further degraded with acid at last.After expanding, material after the processing makes slurry with colloid mill in water.
When making plural layers, can the softish slurry that contains collagen I I be added on the cellulosic face of the smooth film for preparing according to as described in WO-A-95/18638.Under the normal condition, thin film should granule faces down and lies on the even curface, and collagen I I slurry can be applied on the cellulosic face of thin film with the method for for example smearing easily.Slurry forms one deck of any desired thickness, securely attached on the collagen film.The bilayer film that will so form carries out freezing and lyophilizing then, obtains having the spongy texture of needed pore size.If desired, remove some hypothallus possibly to obtain a uniform bilayer film of thickness.In order to make a three-layer thin-film, the thin film that the second layer is smooth be put into hypothallus above, its cellulosic face contacts with hypothallus.
The collagen I I slurry that is added on the thin film generally contains 1.0 to 4.0% (weight), advantageously contains the collagen of 2 to 3% (weight).The pH value of this mixture generally should adjust to 2.5 to 4.5, is preferably 3.0 to 4.0.
Preferably collagen I I material carries out crosslinked after step of freeze drying so that hypothallus is stable.Doing so also can increase the mechanical stability of hypothallus and reduce it by the resorbent speed of health.Ideal crosslinking degree should make the degradation rate of hypothallus and the regeneration rate of tissue be complementary.Can by heat such physical action implement crosslinked, but must careful operation to avoid again the nonconforming loss of absorbability.Preferably be heated to 100 to 120 ℃, kept 30 minutes to 5 hours.The crosslinked method of more preferred enforcement is to use vitalight lamp irradiation the most nearly 8 hours.
Collagen I I material advantageously contains glycosaminoglycan.The latter's practical function is to react with collagen I I and finish some crosslinked action and generate a kind of insoluble product.If desired, also available above-mentioned with the material heating or implement further crosslinked with the method for ultraviolet radiation.Reaction between glycosaminoglycan and collagen can be carried out under room temperature and pH value are 2.5 to 3.5 condition.The content of glycosaminoglycan can be between 1 to 10% (weight).After above-mentioned processing, must carry out freezing to material at once and the lyophilizing processing.
On the other hand, also can generate slurry with the method for rising collagen I I material (mass) pH value.In this process, material is cooled to about 4 ℃, under this temperature, add cold aqueous NaOH then pH value is risen in 6.5 to 7.5 scopes slowly.Material is at room temperature kept 15 to 25 hours subsequently.At this moment just generated slurry, slurry just can carry out freezing and lyophilizing with material after generating.
Also having a kind of alternative method is after removing air, in and collagen I I material make its pH value reach the scope of 6.8-7.4.Again mixture was put in the mould under 37 ℃ of temperature incubation 15 to 20 hours, and just obtained a kind of meticulous slurry, just can carry out freezing and lyophilizing subsequently it.
Performance according to required product is selected in above three kinds of methods.First method can obtain the most stable product, exists yet have caking in the precipitate, therefore must operate very carefully.Second method can obtain softness and uniform product, yet than the easier dissolving of the product of first method.
In the production of slurry, add some materials possibly in addition, for example antimicrobial drug (Taurolidine) or antibiotic (as gentamycin) such as needs such as medicines.
Just carry out freezing after being applied to slurry on the thin film to material.In order to obtain the pore size of good reproducibility, must control refrigerating process carefully, refrigerated speed and time, pH value and particle size also will accurately be controlled.In order to obtain very little micropore, material must freeze suddenly in low-down temperature.
The thin film that will freeze then carries out lyophilizing and is heated to 110 to 130 ℃ subsequently, finishes in this way that some is crosslinked.Can repair the biofilm after the lyophilizing subsequently and reach required thickness.The thickness of hypothallus is generally about 2 millimeters.Then to bilayer film with carrying out sterilization treatment as gamma-rays or oxirane.When with the dosage of 25kGy thin film being carried out sterilization treatment with the such strong ray of cobalt-60, BMPs may inactivation.In this case, aseptic substrate will pour into BMPs in Sterile Saline before implanting.
Thin film of the present invention can be used for medicine by following approach:
Material as guided tissue regeneration.Hypothallus can promote the cell growth, and its barrier layer can suppress the growth of undesirable cell.
As the cartilage defects patching material, that is do not penetrate the patching material of cartilage lower plate (subchondral plate) pathological changes and the patching material of bone cartilage (osteochondral) defective.
The present invention also provides with above-mentioned multilamellar collagen film and has come guided tissue regeneration.Contained collagen I I composition is specially adapted to the regeneration of cartilaginous tissue in this thin film, also is applicable to the tissue regeneration of other type.
The present invention also provides with the implant of foregoing thin film as guided tissue regeneration.
It is a kind of in the human body or the method handled of the intravital bone of inhuman animal or cartilage defects that the present invention also provides, this method comprises to defective uses a kind of foregoing thin film, and the orientation of this thin film will guarantee that its barrier layer can prevent that undesirable types of organization from inwardly growing in bone or the regenerating bone or cartilage zone.
Following examples only provide for explanation.In an embodiment, in steps all must be in aseptic environments as the room of cleaning in implement.
Embodiment 1
(A) muscle and the oils and fats that will take from the calf peritoneum on one's body with mechanical means goes only fully, washing and handling 12 hours in the solution of 2% NaOH in mobile water.And then thin film washed in mobile water and be that 0.5% HCl carries out acidify with concentration.The whole thickness of thin film all after the acidify (about 3 hours) wash until pH value and reach 3.5.Then thin film is shunk NaHCO with 1% with 7% saline solution 3The solution neutralization is also washed in circulating water.Then with material with acetone dehydration and use defat with n-hexane.
(B) the refrigerated pig cartilage on one's body of newly butchering of taking from is immersed in the cold water fully to clean and to be purified to mechanical means and does not have remaining meat, bone and lump.Subsequently material was washed 30 minutes in mobile water.
Subsequently material is placed in the homogenizer and grinds three times.When end reduced in size, particulate visual size is about 8 millimeters.
Then the cartilage piece is washed 4 times with acetone and dewatered each 8 hours.With 4 defats of n-hexane extraction of cartilage piece, handled at least 8 hours subsequently, the ratio of hexane and cartilage is 1: 10 at every turn.
After the defat, cartilage is placed in the drinking water expands, the ratio of water and material is 10: 1, and the processing time is 24 hours.
Then the NaOH of material with 5% (weight) handled, in 32 hours processing times, the ratio of cartilage and solution is 1: 4.The cartilage piece will well stir during processing.Subsequently alkali is washed out from cartilage, pH value drops to 9-11 from initial 14.Dissolved impurity is washed out and is separated with cartilage.The formed residual liquid of alkali treatment will collect to reclaim glycosaminoglycan.
Use the dense HCl of 3% (weight) to handle collagen-based materials then, initial pH value is 1.0, processing time 4-6 hour.
Subsequently material is used the cold water thorough washing, made pH value be raised to 3-3.5.All impurity are removed, and product becomes the collagen material that spongy or other collagen-based materials are made in salt-free being suitable for.For this reason, according to final desired result, this collagen material will outgas, freezing and lyophilizing.
Contain glycosaminoglycan, alkali, denatured protein and salt in the extract of above-mentioned alkali treatment gained.This extract at first will neutralize with HCl, and the pH value after the neutralization is 6.Handle with super-cell then, this filter aid can be removed denatured protein.The kieselguhr of 0.5 weight % joined in the extract and itself and denatured protein are come along and remove with filter method.
Submit supernatant to ultrafiltration subsequently, the weight shutoff value of used ultrafilter membrane is about 10,000 dalton.Remove salinity in this way, what stay is the glycosaminoglycan that has purified.
With the glycosaminoglycan solution that so obtains and top resulting collagen-based materials mutually fusion just become the collagen I I substrate that contains glycosaminoglycan.
The character of collagen I I material is as follows:
TG=2.8% (weight)
GAG=3% (weight) (is basic calculation with collagen)
PH value 3.5
(C) evenly soaking in water by above-mentioned (A) fresh peritoneum thin film of making and shakeouing on glass plate, cellulosic faces up.Subsequently will be thoroughly drenched thin film by above-mentioned (B) collagen I I material of making.Thin film is launched straightly and attaches onboard to all directions.Like this, collagen I I material can infiltrate in the thin film.
After having smeared a thick-layer material on the thin film glass plate is put into refrigerator, under about 4 ℃ of temperature, keep a whole night.Just formed slurry during this period.
Slurry is freezing under following condition:
Bath temperature=-12 ℃
Time=40 minute
Subsequently freezing slurry is carried out lyophilizing, be heated to 125 ℃ then.
Freeze-drying time=14 hour.
Then collagen I I hypothallus being split into is 1 millimeter thickness.
Embodiment 2
The fresh peritoneum thin film of making by example 1 (A) is applied on the glass plate, and the thick collagen I I material that will have example 1 described character rubs in the thin film.With 50 gram collagen I I materials with distilled water diluting to 100 milliliter and thoroughly stir.The glycosaminoglycan solution that when stirring, adds 100 milliliters lentamente.Collagen becomes integral body with GAG and precipitates.Precipitating later material is homogenize.So the dispersion of gained is applied on the thin film.Through just having formed slurry a night, further process by example 1 described method through the thin film of so handling.

Claims (13)

1. one kind is applicable to the plural layers of rebuilding in bone or the cartilaginous tissue body, this thin film comprises: (i) one deck is mainly collagen I I and has the hypothallus of open spongy texture, the (ii) barrier layer of one deck with closed construction at least, this barrier layer is impervious, be enough to stop cell passes and to wherein the growth, described one deck at least barrier layer is mainly made by I type and III Collagen Type VI, and described thin film is by collagen I I slurry being applied to the surface on described barrier layer, makes described collagen I I hypothallus have that open spongiosis makes by lyophilizing subsequently.
2. thin film as claimed in claim 1, this thin film comprises single barrier layer.
3. thin film as claimed in claim 1, hypothallus wherein is arranged between the two-layer barrier layer.
4. thin film as claimed in claim 1, wherein said collagen I I material is taken from natural cartilage.
5. thin film as claimed in claim 4, collagen I I material is wherein taken from the hyaline cartilage of pig.
6. claim 4 or 5 thin film, collagen I I material wherein is through heating or crosslinked with ultraviolet radiation.
7. thin film as claimed in claim 1, one or more barrier layer are the peritoneums of taking from calf or pig.
8. thin film as claimed in claim 1, hypothallus infiltration wherein has separation from articular cartilage, periosteum, perichondral chondrocyte or myeloid interstital stem cell.
9. thin film as claimed in claim 1, wherein the infiltration of at least one hypothallus and/or barrier layer has glycosaminoglycan.
10. thin film as claimed in claim 9, glycosaminoglycan wherein are hyaluronic acid, chrondroitin 6-sulfate, keratin sulfate or dermatan sulfate.
11. thin film as claimed in claim 1, hypothallus wherein and barrier layer do not have Dan Baijutang basically.
12. thin film as claimed in claim 1, wherein one deck hypothallus and/or barrier layer also comprise chondronectin, laminin, fibronectin, calcium alginate, anchorin-II, somatomedin or bone morphogenic factor at least.
13. the purposes of the thin film of claim 1 in the implant of making guided tissue regeneration.
CNB988117347A 1997-10-10 1998-10-05 Membrane for use in guided tissue regeneration Expired - Fee Related CN1181893C (en)

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