CN117941655B - Efficient breeding method for culex pallidum - Google Patents
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Abstract
The application discloses a high-efficiency breeding method of culex pallidum, which relates to the technical field of mosquito raising, wherein S1, eclosion: putting the mosquito pupae of the culex pallidum after the male and female are separated into an adult mosquito rearing cage; s2, spawning: after the mosquito pupas are fully feathered into adult mosquitoes for 5 days, feeding female mosquitoes with anticoagulated sheep blood, and placing spawning cups in an adult mosquito rearing cage after feeding the blood for the female mosquitoes to spawn; s3, hatching: adding water into the pig liver leaching solution and yeast powder to prepare a nutrient solution; adding mosquito eggs into an incubator, adding nutrient solution into the incubator, and transferring the hatched larvae into a feeding tray; s4, larva feeding: putting the hatched culex larvae into a feeding tray for feeding until pupation; wherein the fermentation decomposes the nutritional ingredients in the raw materials into small molecules which are easier to eat and reduce anti-nutritional factors and harmful substances; solves the problem that the growth and development of culex pallidum larvae are blocked or dead caused by mixed bacteria or harmful bacteria introduced from a nutrition source, and achieves the effects of improving the hatching rate and reducing pollution.
Description
Technical Field
The invention relates to the technical field of mosquito raising, in particular to a high-efficiency breeding method of culex pallidum.
Background
The culex pallidum (Culex PIPIENS PALLENS) is an insect of culex genus of mosquito family, the culex pallidum is moderate in shape, light brown, the beak and foot are dark brown, no white ring exists, and the middle chest backboard has no white stripes; the base of each abdomen and back section is provided with a gray transverse belt, and the rear edge of the belt is flat and straight; culex pallidum mainly grows in moderately polluted ponding near human habitats. Sewage pools, foul water ditches, septic tanks, rainwater well ponds, sewer ponds, fertilizer water jars for watering flowers and pit ponds of construction sites in cities are important breeding places for mosquitoes; artificial feeding of insects is one of the basic technologies for studying the entomology, and can be used for breeding a certain kind of target insects without being limited by seasons, so as to be used for studying the aspects of insect biology, physiology, pest comprehensive control measure establishment and the like; the culex can spread diseases such as filariasis and epidemic encephalitis B, and the mosquito medium control measures for controlling the quantity of spread viruses so as to reduce the contact of the culex with human are of great significance.
The breeding method of Chinese patent, bulletin number, CN107372371B and aedes albopictus spawning female mosquitoes comprises the following steps: placing the mosquito pupas of the aedes albopictus into adult mosquito feeding cages, enabling the mosquito pupas to emerge into adult mosquitoes, and placing the female pupas of the aedes albopictus and the male pupas of the aedes albopictus into each adult mosquito feeding cage in a quantity ratio of 1-5:1; the method can obtain higher spawning rate, the obtained mosquito eggs are high in quality, the larva hatching rate is high, the spawning efficiency of the aedes albopictus is greatly improved, the method can be used as a standard raising method of the female aedes albopictus, and powerful guarantee is provided for the mass production of the aedes albopictus in laboratories and factories so as to solve the problem of insufficient spawning in the release production process of the male aedes albopictus.
However, in the breeding process of culex pallidum, animal liver leachate is commonly used as a nutrient solution, animal liver may carry mixed bacteria or harmful bacteria, and the animal liver may be propagated in a large amount in the fermentation process, competing with culex larva or insect pupa for nutrition resources, changing the water environment, consuming oxygen in water, generating harmful substances at the same time, and even directly parasitizing, so that the growth and development of the mosquito larva or insect pupa are blocked or dead.
Disclosure of Invention
The embodiment of the application solves the problem that the growth and development of culex pallidum larvae are blocked or dead caused by mixed bacteria or harmful bacteria introduced by a nutrient source in the prior art by providing the efficient breeding method of culex pallidum, and achieves the technical effects of improving the hatching rate and reducing pollution.
The embodiment of the application provides a high-efficiency breeding method for culex pallidum, which comprises the following steps:
s1, feathering: placing the mosquito pupae of the culex pallidum after the separation of the male and female in a mosquito raising cage at the temperature of 25-30 ℃ and the relative humidity of 60-80%, wherein the number ratio of the female and male of the culex pallidum to the male of the culex pallidum is 3:1, so that the mosquito pupae is eclosion to be a mosquito, and providing cotton balls containing glucose aqueous solution with the mass concentration of 15% for the mosquito during the eclosion period;
S2, spawning: after the mosquito pupas are fully feathered into adult mosquitoes for 5 days, feeding female mosquitoes with anticoagulated sheep blood, and placing spawning cups in an adult mosquito rearing cage after feeding the blood until the female mosquitoes spawn for the first time; collecting the mosquito eggs in the spawning cup for the first spawning after three days, feeding the female mosquito with anticoagulated sheep blood for the second time, repeating the first feeding step, and finally collecting the mosquito eggs produced for the second time;
Wherein the temperature of sheep blood is maintained at 37 ℃ and the feeding time is 0.5-2h;
S3, hatching: adding water into the pig liver leaching solution and yeast powder to prepare a nutrient solution;
Adding the mosquito eggs into an incubator, and adding nutrient solution into the incubator; sealing a tube cover of the hatching tube, shaking uniformly, standing and hatching for 1 hour at the temperature of 25-30 ℃, then opening the tube cover of the hatching tube, and transferring the hatched larvae into a feeding tray;
The nutrient solution comprises the following components: pig liver leachate: 19% (by weight), yeast powder: 1% (by weight), water: 79% (by weight);
The preparation method of the nutrient solution comprises the following steps: cutting fresh pork liver into 2 x 2cm small pieces, placing the small pieces into a container filled with water, wherein the mass ratio of the pork liver to the water is 1:7.5-8.5, the initial temperature is 60-65 ℃ in the process, the time is 0.5-1 hour, the subsequent cooling is slowly reduced to 30 ℃, the pork liver leaching liquid is obtained after the period of 4-6 hours, and then yeast powder is added according to 5.2-5.3% of the mass of the solution in the container, namely the nutrient solution;
s4, larva feeding: feeding the hatched culex larvae into a feeding tray according to the feeding density of 300 larvae/tray until pupation;
Further, in step S4, larval rearing: feeding the hatched culex larvae into a feeding tray according to the feeding density of 300 larvae/tray, and feeding by using mixed solution of feed solution and nutrient solution in the feeding process until pupation;
Wherein the feed liquid is obtained by fermenting nutrient solution; the specific fermentation time is 20-26h, and the temperature is 25-30 ℃; the weight ratio of the raising liquid to the nutrient solution is 1:2.
Further, in the step S4, the feeding chamber is placed in a feeding tray, and mixed solution of feeding solution and nutrient solution is sequentially injected, wherein the specific proportion of the nutrient solution in the first feeding chamber is as follows by weight: pig liver leachate: 15%, yeast powder: 1%, water: 84%; the specific proportion of the nutrient solution in the second feeding cavity is as follows by weight: pig liver leachate: 19%, yeast powder: 1%, water: 80%; the specific proportion of the nutrient solution in the third feeding cavity is as follows by weight: pig liver leachate: 23%, yeast powder: 1%, water: 76% and allows the larvae to be more in a more suitable nutritional environment.
Further, in step S4, 300 larvae are firstly divided into different feeding chambers on average, and larvae in other feeding chambers are observed and placed into the feeding chamber with highest larva activity.
Further, the feeding cavity in the step S4 is filled with the mixed solution of the nutrient solution and the feeding solution until the convex liquid surface protruding out of the cavity opening exists.
Further, after the culex larvae are put into the feeding tray for feeding, the culex larvae are firstly placed into the first feeding cavity, and after the culex larvae rise to the liquid level for breathing for many times, the culex larvae gradually enter the second feeding cavity, and continuously enter the next feeding cavity in the process of continuously improving the nutrition requirements.
Further, when more than 95% of the larvae enter the second feeding chamber, the solution in the first feeding chamber is replaced by a new solution.
Further, when the first feeding cavity is replaced by a new solution, a new batch of larvae is put in.
Further, in the step S4, after the culex larvae are put into a feeding tray for feeding, the culex larvae are firstly placed into a central feeding cavity, and after the culex larvae rise to the liquid level for breathing for many times, the culex larvae with different requirements gradually enter into different feeding cavities.
Further, the same feeding cavity is partitioned again, and different ratios of feed liquid and nutrition are set.
One or more technical solutions provided in the embodiments of the present application at least have the following technical effects or advantages:
Firstly, the nutrition components in the raw materials are decomposed into small molecules by fermentation so as to be easier to eat, anti-nutritional factors and harmful substances are reduced, a proper environment is provided for enzymes in the pig liver at 30 ℃, so that the enzymes can be gradually activated and act on the proteins, the lipids and other components in the pig liver, the small molecule nutrition substances are further released, the leaching degree of the nutrition substances in the pig liver is improved at high temperature, a plurality of macromolecular proteins are primarily decomposed, and the survival rate of the hatched larvae and the absorption and utilization degree of the nutrition are improved; the temperature of the pig liver leaching process is high in the initial process, so that mixed bacteria or harmful bacteria brought by the pig liver leaching liquid are reduced, and the possibility of pollution caused by the mixed bacteria and the harmful bacteria in the pig liver is reduced.
Secondly, the nutrition components in the fresh pig liver leaching solution and the fermented pig liver leaching solution have complementary effects, the fermented feed liquid has a certain antibacterial capacity, the unfermented nutrition liquid contains less mixed bacteria, and the two are mixed, so that the difference degree of changing living environment of larvae is reduced, and the overall nutrition comprehensiveness and antibacterial capacity are improved.
Thirdly, by arranging a plurality of feeding chambers and configuring nutrient solutions with different concentrations for each feeding chamber, the requirements of different culex larvae on nutrition in different growth stages can be met; along with the growth and peeling of the larvae, the demands of the larvae on nutrition are gradually increased, so that the larvae can gradually enter a feeding cavity with higher nutrition concentration, the larvae can be initially differentiated, and the larvae with different growth degrees can be selected; when most larvae (more than 95 percent) enter the second feeding cavity, the solution in the first feeding cavity is replaced by a new solution, and a new batch of larvae is put into the first feeding cavity, so that the efficient utilization of feeding resources is realized, waste is avoided, and meanwhile, the cleaning and sanitation of the feeding environment can be kept by continuously replacing and updating the solution, and the healthy growth of the larvae is facilitated; the solution in the feeding cavity is filled up until the raised liquid surface protruding out of the cavity opening provides more breathing space for the larvae, so that the larvae are prevented from being lack of oxygen for a long time under the liquid surface, the larvae can enter the next feeding cavity as a channel, the larvae can conveniently move between different feeding cavities, the larvae automatically enter the most suitable environment, and the overall growth speed of the larvae is improved.
Fourthly, by arranging the circular feeding tray, the larvae can freely enter a proper growth environment, and as the nutritional requirements of the mosquitoes for proper growth in different seasons and at different temperatures are different, the nutritional requirements of the larvae in different environment conditions and even the requirements of different types of mosquitoes can be met by providing a plurality of pig liver lixivium with different concentrations, so that the feeding process is more flexible and personalized, and the growth speed and health condition of the larvae can be improved; by allowing the larvae to select which feeding cavity to enter by themselves, a certain degree of self-regulation of nutrient intake of the larvae is realized, the frequency and degree of manual intervention are reduced, the feeding cost is reduced, and the feeding efficiency is improved. Because the nutrient solution of each feeding cavity is proportioned according to specific requirements, waste and pollution can be avoided.
Drawings
FIG. 1 is a schematic view of a fourth feeding chamber according to an embodiment of the present invention;
FIG. 2 is a schematic view of a circular feeding chamber of the present invention;
Detailed Description
In order that the application may be readily understood, a more complete description of the application will be rendered by reference to the appended drawings; the preferred embodiments of the present application are illustrated in the drawings, but the present application can be embodied in many different forms and is not limited to the embodiments described herein; rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs; the terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention; the term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
Example 1
The application relates to a high-efficiency breeding method of culex pallidum, which comprises the following steps:
s1, feathering: placing the mosquito pupae of the culex pallidum after the separation of the male and female in a mosquito raising cage at the temperature of 25-30 ℃ and the relative humidity of 60-80%, wherein the number ratio of the female and male of the culex pallidum to the male of the culex pallidum is 3:1, so that the mosquito pupae is eclosion to be a mosquito, and providing cotton balls containing glucose aqueous solution with the mass concentration of 15% for the mosquito during the eclosion period;
S2, spawning: after the mosquito pupas are fully feathered into adult mosquitoes for 5 days, feeding female mosquitoes with anticoagulated sheep blood, and placing spawning cups in an adult mosquito rearing cage after feeding the blood until the female mosquitoes spawn for the first time; collecting the mosquito eggs in the spawning cup for the first spawning after three days, feeding the female mosquito with anticoagulated sheep blood for the second time, repeating the first feeding step, and finally collecting the mosquito eggs produced for the second time;
Wherein the temperature of sheep blood is maintained at 37 ℃ and the feeding time is 0.5-2h;
S3, hatching: adding water into the pig liver leaching solution and yeast powder to prepare a nutrient solution;
Adding the mosquito eggs into an incubator, and adding nutrient solution into the incubator; sealing a tube cover of the hatching tube, shaking uniformly, standing and hatching for 1 hour at the temperature of 25-30 ℃, then opening the tube cover of the hatching tube, and transferring the hatched larvae into a feeding tray;
The nutrient solution comprises the following components: pig liver leachate: 19% (by weight), yeast powder: 1% (by weight), water: 79% (by weight);
The preparation method of the nutrient solution comprises the following steps: cutting fresh pork liver into 2 x 2cm small pieces, placing the small pieces into a container filled with water, wherein the mass ratio of the pork liver to the water is 1:7.5-8.5, the initial temperature is 60-65 ℃ in the process, the time is 0.5-1 hour, the subsequent cooling is slowly reduced to 30 ℃, the pork liver leaching liquid is obtained after the period of 4-6 hours, and then yeast powder is added according to 5.2-5.3% of the mass of the solution in the container, namely the nutrient solution;
s4, larva feeding: feeding the hatched culex larvae into a feeding tray according to the feeding density of 300 larvae/tray, and feeding by using a feeding solution in the feeding process until pupation;
wherein the feed liquid is obtained by fermenting nutrient solution; the fermentation time is 20-26h, and the temperature is 25-30 ℃.
The technical scheme provided by the embodiment of the application at least has the following technical effects or advantages:
The fermentation ensures that the nutritional ingredients in the raw materials are decomposed into small molecules which are easier to eat, and anti-nutritional factors and harmful substances are reduced, and a proper environment is provided for enzymes in the pig liver at 30 ℃, so that the enzymes can be gradually activated and act on the proteins, the lipids and other ingredients in the pig liver, the small molecular nutritional substances are further released, the leaching degree of the nutritional substances in the pig liver is improved at high temperature, some macromolecular proteins are primarily decomposed, and the survival rate of the larvae after hatching and the absorption and utilization degree of the nutrition are improved; the temperature of the pig liver leaching process is high in the initial process, so that mixed bacteria or harmful bacteria brought by the pig liver leaching liquid are reduced, and the possibility of pollution caused by the mixed bacteria and the harmful bacteria in the pig liver is reduced.
Example two
The embodiment improves the survival rate of the larvae after hatching by using the fermented pig liver leaching liquid, but the nutrient solution and the feeding solution have the problem that the larvae just hatched may have poor adaptability due to the large component change after fermentation, and further improves the method.
S4, larva feeding: feeding the hatched culex larvae into a feeding tray according to the feeding density of 300 larvae/tray, and feeding by using mixed solution of feed solution and nutrient solution in the feeding process until pupation;
Wherein the feed liquid is obtained by fermenting nutrient solution; the specific fermentation time is 20-26h, and the temperature is 25-30 ℃; the weight ratio of the raising liquid to the nutrient solution is 1:2.
The technical scheme provided by the embodiment of the application at least has the following technical effects or advantages:
the nutrient components in the fresh pig liver leaching solution and the fermented pig liver leaching solution have complementary effects, the fermented feed liquid has a certain antibacterial capability, the unfermented nutrient liquid contains less mixed bacteria, and the mixed nutrient liquid not only reduces the difference degree of the replacement living environment of larvae, but also improves the overall nutrition comprehensiveness and antibacterial capability.
Example III
The feeding solution after fermentation is mixed with the nutrient solution, so that the adaptability of the larvae is improved, and the method is further improved for further meeting the nutrition required by the larvae with different growth degrees and peeling times in the feeding process, simplifying the operation of the breeding process.
S4, larva feeding: feeding the hatched culex larvae into a feeding tray according to the feeding density of 300 larvae/tray, and feeding by using mixed solution of feed solution and nutrient solution in the feeding process until pupation;
wherein the feed liquid is obtained by fermenting nutrient solution; the specific fermentation time is 20-26h, and the temperature is 25-30 ℃; the weight ratio of the feed liquid to the nutrient solution is 1:2;
The feeding tray is shown in fig. 1, a plurality of feeding chambers are arranged in parallel, mixed solution of feeding solution and nutrient solution is sequentially injected, and the specific proportion of the nutrient solution in the first feeding chamber is as follows by weight: pig liver leachate: 15%, yeast powder: 1%, water: 84%; the specific proportion of the nutrient solution in the second feeding cavity is as follows by weight: pig liver leachate: 19%, yeast powder: 1%, water: 80%; the specific proportion of the nutrient solution in the third feeding cavity is as follows by weight: pig liver leachate: 23%, yeast powder: 1%, water: 76%; the solution in the feeding cavity is added to two thirds, 300 larvae are firstly evenly divided into different feeding cavities, the activity of the larvae in the second feeding cavity is found to be highest, the larvae in other feeding cavities are placed into the second feeding cavity, and the larvae in the subsequent feeding cavities are placed into the second feeding cavity.
The technical scheme provided by the embodiment of the application at least has the following technical effects or advantages:
through setting up a plurality of rearing the chamber to for every rearing the chamber and disposing the nutrient solution of different concentration, can satisfy the demands of different culex larvae to nutrition in different growth stages.
Example IV
Further improvements were made to further optimize the ability of the larvae to select for the environment.
S4, larva feeding: feeding the hatched culex larvae into a feeding tray according to the feeding density of 300 larvae/tray, and feeding by using mixed solution of feed solution and nutrient solution in the feeding process until pupation;
wherein the feed liquid is obtained by fermenting nutrient solution; the specific fermentation time is 20-26h, and the temperature is 25-30 ℃; the weight ratio of the feed liquid to the nutrient solution is 1:2; the specific proportion of the nutrient solution in the first feeding cavity is as follows by weight: pig liver leachate: 15%, yeast powder: 1%, water: 84%; the specific proportion of the nutrient solution in the second feeding cavity is as follows by weight: pig liver leachate: 19%, yeast powder: 1%, water: 80%; the specific proportion of the nutrient solution in the third feeding cavity is as follows by weight: pig liver leachate: 23%, yeast powder: 1%, water: 76% adding mixed solution of nutrient solution and raising solution into the cavity until there is convex liquid surface protruding from the cavity mouth, after the culex larvae are put into the raising tray for raising, firstly placing them into the first raising cavity, when the feeding quantity of some larvae is increased after the larvae grow up and are peeled or the first raising cavity is insufficient in nutrition, after the larvae rise to the liquid surface for several times, they can be gradually fed into the second raising cavity, and under the process of continuously raising nutrient requirement, they also can be continuously fed into the next raising cavity, when more than 95% of larvae are fed into the second raising cavity, the solution in the first raising cavity is replaced with new solution, and a new batch of larvae is placed.
The technical scheme provided by the embodiment of the application at least has the following technical effects or advantages:
Along with the growth and peeling of the larvae, the demands of the larvae on nutrition are gradually increased, so that the larvae can gradually enter a feeding cavity with higher nutrition concentration, the larvae can be initially differentiated, and the larvae with different growth degrees can be selected; when most larvae (more than 95 percent) enter the second feeding cavity, the solution in the first feeding cavity is replaced by a new solution, and a new batch of larvae is put into the first feeding cavity, so that the efficient utilization of feeding resources is realized, waste is avoided, and meanwhile, the cleaning and sanitation of the feeding environment can be kept by continuously replacing and updating the solution, and the healthy growth of the larvae is facilitated; the solution in the feeding cavity is filled up until the raised liquid surface protruding out of the cavity opening provides more breathing space for the larvae, so that the larvae are prevented from being lack of oxygen for a long time under the liquid surface, the larvae can enter the next feeding cavity as a channel, the larvae can conveniently move between different feeding cavities, the larvae automatically enter the most suitable environment, and the overall growth speed of the larvae is improved.
Example five
The embodiment is characterized in that the four sides of the feeding cavity are filled with the solution until the solution is protruded out of the convex liquid surface of the cavity opening, so that larvae can move freely in different cavities, and the best environment is automatically found, so that the universality of the larvae in different environments and different kinds of mosquitoes is further improved.
As shown in fig. 2, the feeding tray is annular, except that a central feeding area is arranged in the middle, the periphery is divided into 8 areas, two opposite areas are provided, the nutrition liquid ratio is the same, the partition walls of adjacent areas are higher than the partition walls of the partition areas and the central feeding area, the weight ratio of the used feeding liquid to the nutrition liquid mixed solution is 1:2, and the specific ratio of the nutrition liquid in the central feeding cavity is as follows: pig liver leachate: 19%, yeast powder: 1%, water: 80%; the nutrient solution of the feeding cavity A comprises the following components in parts by weight: pig liver leachate: 18.5%, yeast powder: 1%, water: 80.5%; the nutrient solution of the feeding cavity B comprises the following components in parts by weight: pig liver leachate: 18%, yeast powder: 1%, water: 81% of a glass fiber; the nutrient solution of the feeding cavity C comprises the following components in parts by weight: pig liver leachate: 19.5%, yeast powder: 1%, water: 79.5%; the specific proportion of the nutrient solution of the feeding cavity D is as follows by weight: pig liver leachate: 20% of yeast powder: 1%, water: 79%; adding mixed solution of nutrient solution and raising solution into the cavity until there is convex liquid surface protruding out of the cavity mouth, putting the culex larva into the raising tray for raising, putting the culex larva into the central raising cavity, and gradually entering into different raising cavities after the culex larva with different requirements rises to the liquid surface for breathing for many times.
The larvae with different adaptability can be selected by partitioning the same feeding cavity again and setting the proportion of different feeding solutions and nutrients.
The technical scheme provided by the embodiment of the application at least has the following technical effects or advantages:
Through setting up circular feeding tray for the larva freely gets into suitable growing environment, because the nutrition demand of the suitable growth of mosquito in different seasons, under the different temperatures is different, through providing the pig liver leaching liquid of multiple different concentration, can satisfy the nutrition demand of larva under the different environment condition, even the demand of different kinds of mosquitoes, makes the raising process more nimble and individualized, helps improving the growth rate and the health condition of larva.
By allowing the larvae to select which feeding cavity to enter by themselves, a certain degree of self-regulation of nutrient intake of the larvae is realized, the frequency and degree of manual intervention are reduced, the feeding cost is reduced, and the feeding efficiency is improved. Because the nutrient solution of each feeding cavity is proportioned according to specific requirements, waste and pollution can be avoided. The difference between the comparative example I and the example I is that the pig liver leaching liquid is obtained by leaching fresh pig liver in water at 25 ℃ for 6-8 hours.
The results of the egg hatching rate, larva development time, adult rate and total hatching rate of the mosquito eggs are counted as a table 1 larva development time comparison table, a table 2 adult mosquito spawning amount and egg hatching rate comparison table, and a table 3 adult rate and total hatching rate comparison table; the egg hatching rate is the ratio of the number of the hatched larvae to the total number of the mosquito eggs used for hatching; the larva development time refers to the time required for the hatched larva to develop to pupate; the adult rate refers to the proportion of larvae developing to adults; total hatchability refers to the ratio of eggs to adults.
Table 1 comparison of larval development time table
TABLE 2 comparison of egg laying amount and egg hatching rate of adult mosquitoes
TABLE 3 comparison of the adult rate and the total hatchability
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. The efficient breeding method of culex pallidum is characterized by comprising the following steps:
s1, feathering: placing the mosquito pupae of the culex pallidum after the separation of the male and female in a mosquito raising cage at the temperature of 25-30 ℃ and the relative humidity of 60-80%, wherein the number ratio of the female and male of the culex pallidum to the male of the culex pallidum is 3:1, so that the mosquito pupae is eclosion to be a mosquito, and providing cotton balls containing glucose aqueous solution with the mass concentration of 15% for the mosquito during the eclosion period;
S2, spawning: after the mosquito pupas are fully feathered into adult mosquitoes for 5 days, feeding female mosquitoes with anticoagulated sheep blood, and placing spawning cups in an adult mosquito rearing cage after feeding the blood until the female mosquitoes spawn for the first time; collecting the mosquito eggs in the spawning cup for the first spawning after three days, feeding the female mosquito with anticoagulated sheep blood for the second time, repeating the first feeding step, and finally collecting the mosquito eggs produced for the second time;
Wherein the temperature of sheep blood is maintained at 37 ℃ and the feeding time is 0.5-2h;
S3, hatching: adding water into the pig liver leaching solution and yeast powder to prepare a nutrient solution;
Adding the mosquito eggs into an incubator, and adding nutrient solution into the incubator; sealing a tube cover of the hatching tube, shaking uniformly, standing and hatching for 1 hour at the temperature of 25-30 ℃, then opening the tube cover of the hatching tube, and transferring the hatched larvae into a feeding tray;
the nutrient solution comprises the following components: wherein the nutrient solution comprises the following components in weight: pig liver leachate: 19%, yeast powder: 1%, water: 79%;
The preparation method of the nutrient solution comprises the following steps: cutting fresh pork liver into small blocks with the mass ratio of 2 multiplied by 2cm, putting the small blocks into a container filled with water, wherein the mass ratio of the pork liver to the water is 1:7.5-8.5, the initial temperature is 60-65 ℃ in the process, the time is 0.5-1 hour, the subsequent cooling is slowly reduced to 30 ℃, the liquid is maintained for 4-6 hours, and then yeast powder is added according to the mass ratio of 5.2-5.3% of the solution in the container, so that the liquid is the nutrient solution;
s4, larva feeding: feeding the hatched culex larvae into a feeding tray according to the feeding density of 300 larvae/tray, and feeding by using mixed solution of feed solution and nutrient solution in the feeding process until pupation;
wherein the feed liquid is obtained by fermenting nutrient solution; the specific fermentation time is 20-26h, and the temperature is 25-30 ℃; the weight ratio of the feed liquid to the nutrient solution is 1:2;
Placing the feeding cavity in a feeding tray, sequentially injecting mixed solution of feeding solution and nutrient solution, wherein the specific proportion of the nutrient solution in the first feeding cavity is as follows: pig liver leachate: 15%, yeast powder: 1%, water: 84%; the specific proportion of the nutrient solution in the second feeding cavity is as follows by weight: pig liver leachate: 19%, yeast powder: 1%, water: 80%; the specific proportion of the nutrient solution in the third feeding cavity is as follows by weight: pig liver leachate: 23%, yeast powder: 1%, water: 76% and more larvae are located in a more suitable nutritional environment;
the feeding cavity is filled with a mixed solution of the nutrient solution and the raising solution until a convex liquid surface protruding out of the cavity opening exists;
after the culex larvae are put into the feeding tray for feeding, the culex larvae are firstly placed into the first feeding cavity, and after the culex larvae rise to the liquid level for breathing for many times, the culex larvae gradually enter the second feeding cavity, and continuously enter the next feeding cavity in the process of continuously improving the nutrition requirements.
2. The efficient breeding method of culex pallidum as claimed in claim 1, wherein when more than 95% of larvae enter the second feeding chamber, the solution in the first feeding chamber is replaced with new solution.
3. The method of claim 2, wherein a new batch of larvae is placed in the first feeding chamber when the first feeding chamber is replaced with a new solution.
4. The efficient breeding method of culex pallidum as claimed in claim 3, wherein the breeding tray is a circular tray, and the step S4 is to put the culex pallidum larvae into the central breeding chamber after feeding, and the larvae with different requirements gradually enter into different breeding chambers after rising to the liquid level for breathing for many times.
5. The efficient breeding method of culex pallidum as claimed in claim 4, wherein the same breeding chamber is partitioned again, and different ratios of breeding liquid and nutrition are set.
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| CN113749056A (en) * | 2021-08-31 | 2021-12-07 | 广州威佰昆生物科技有限公司 | Culex spawning and hatching method |
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| CN101707990A (en) * | 2009-11-27 | 2010-05-19 | 宁夏农林科学院 | Artificial propagation method of Hippodamia variegate |
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