CN107372371A - Breeding method of aedes albopictus oviposition female mosquito - Google Patents
Breeding method of aedes albopictus oviposition female mosquito Download PDFInfo
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- CN107372371A CN107372371A CN201710803964.3A CN201710803964A CN107372371A CN 107372371 A CN107372371 A CN 107372371A CN 201710803964 A CN201710803964 A CN 201710803964A CN 107372371 A CN107372371 A CN 107372371A
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- 241000255925 Diptera Species 0.000 title claims abstract description 139
- 241000256173 Aedes albopictus Species 0.000 title claims abstract description 64
- 238000009395 breeding Methods 0.000 title claims abstract description 28
- 230000017448 oviposition Effects 0.000 title abstract description 6
- 230000012447 hatching Effects 0.000 claims abstract description 59
- 238000000034 method Methods 0.000 claims abstract description 35
- 230000000384 rearing effect Effects 0.000 claims abstract description 23
- 235000013601 eggs Nutrition 0.000 claims abstract description 21
- 102000002322 Egg Proteins Human genes 0.000 claims description 29
- 108010000912 Egg Proteins Proteins 0.000 claims description 29
- 210000004681 ovum Anatomy 0.000 claims description 28
- 239000008280 blood Substances 0.000 claims description 26
- 210000004369 blood Anatomy 0.000 claims description 26
- 241000382353 Pupa Species 0.000 claims description 24
- 230000001488 breeding effect Effects 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 20
- 241001247197 Cephalocarida Species 0.000 claims description 18
- 239000001888 Peptone Substances 0.000 claims description 15
- 108010080698 Peptones Proteins 0.000 claims description 15
- 235000019319 peptone Nutrition 0.000 claims description 15
- 239000007864 aqueous solution Substances 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 108010058643 Fungal Proteins Proteins 0.000 claims description 8
- 241001494479 Pecora Species 0.000 claims description 7
- 238000007710 freezing Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 241000256118 Aedes aegypti Species 0.000 claims description 2
- 230000008144 egg development Effects 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 238000011534 incubation Methods 0.000 description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 7
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 7
- 241000238631 Hexapoda Species 0.000 description 5
- 229920000742 Cotton Polymers 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 208000001490 Dengue Diseases 0.000 description 2
- 206010012310 Dengue fever Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 208000025729 dengue disease Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 241000238582 Artemia Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000004920 integrated pest control Methods 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to a method for feeding aedes albopictus oviposition female mosquitoes, which comprises the following steps: the mosquito pupae of the aedes albopictus are placed into adult mosquito rearing cages to enable the mosquito pupae to emerge into adult mosquitoes, and the number ratio of female aedes albopictus pupae to male aedes albopictus pupae placed into each adult mosquito rearing cage is 1-5: 1. The breeding method can obtain higher spawning rate, the obtained mosquito eggs have high quality, the larva hatching rate is high (especially when the male-female ratio is 3:1, the spawning amount of the female mosquito is the highest), the spawning efficiency of the aedes albopictus is greatly improved, and the breeding method can be used as a standard spawning female mosquito breeding method, provides powerful guarantee for large-scale production of the aedes albopictus in laboratories and factories, and solves the problem of insufficient egg supply in the production process of male aedes albopictus release.
Description
Technical field
The present invention relates to technical field of insect feeding, more particularly to a kind of raising side of the spawning female mosquito of aedes albopictus
Method.
Background technology
It is one of basic fundamental to entomologize manually to raise insect, can not be subject to seasonal restrictions to breed certain species
Purpose insect, for insect biology, physiology and integrated pest control measure formulate etc. research.Aedes albopictus
It is the most important communication media of China mainland area dengue fever virus, distribution latitude is wide.At present for dengue prevalence control most
Good method is exactly to control aedes albopictus density, wherein the male mosquito that Wolbach fungal component is carried by discharging is anti-for a kind of biology
Method is controlled, the population density of field aedes albopictus can be greatly lowered；So provide a kind of artificial extensive raising lineae ablicantes she
The method of mosquito, turns into active demand；It is the method for breeding for needing to establish a kind of spawning female mosquito of standard first, and rarely has both at home and abroad
Reported on mosquito spawning and the correlative study of method for breeding.Method for breeding according to other insects is difficult to improve aedes albopictus production
Feeding efficiency, spawning rate and the laying quality of ovum female mosquito, thus can cause to occur in aedes albopictus hero mosquito release production process
For ovum deficiency the problem of, thus can not large-scale promotion Sterile-insect technique prevent and treat aedes albopictus.
The content of the invention
Based on this, the invention provides a kind of method for breeding of aedes albopictus spawning female mosquito, the method for breeding has higher
Female mosquito spawning rate and laying quality.
Concrete technical scheme is as follows：
A kind of method for breeding of aedes albopictus spawning female mosquito, including the step of emergence：The tumbler of aedes albopictus is put into
In mosquito rearging cage, tumbler is turned into adult mosquito, the female pupa of aedes albopictus and aedes albopictus hero pupa are put into each adult mosquito rearing cage
Quantity ratio be 1~5:1.
Preferably, be put into each adult mosquito rearing cage the quantity ratio of the female pupa of aedes albopictus and aedes albopictus hero pupa for 2~
4:1。
Preferably, the quantity ratio that the female pupa of aedes albopictus and aedes albopictus hero pupa are put into each adult mosquito rearing cage is 2.8
~3.2:1.
Preferably, the quantity ratio that the female pupa of aedes albopictus and aedes albopictus hero pupa are put into each adult mosquito rearing cage is 3:1.
Preferably, the method for breeding of the aedes albopictus spawning female mosquito, comprises the following steps：
Sprout wings：By the quantity of the above-mentioned female pupa of aedes albopictus and aedes albopictus hero pupa than the tumbler of aedes albopictus is put into
In mosquito rearging cage, tumbler is turned into adult mosquito, D/W is provided to adult mosquito during emergence, emergence time is 2-3 days；
Spawning：The tumbler turned into after adult mosquito after 4-6 days completely, blood was fed to female mosquito, in adult mosquito rearing cage after hello blood
Middle spawning cup of placing treats that female mosquito is laid eggs, and the mosquito ovum in spawning cup is collected after spawning；
Hatching：It is larva by mosquito egg hatching after mosquito egg development 6-8 days.
Preferably, the spawning includes：The tumbler was turned into after adult mosquito after 4-6 days completely, and first is carried out to female mosquito
It is secondary to feed blood；
For the first time after one day after hello blood, spawning cup is placed in adult mosquito rearing cage and treats that female mosquito is laid eggs for the first time；
The mosquito ovum in the spawning cup of spawning for the first time is collected two days later, and is carried out second to female mosquito and fed blood；
After one day after second of hello blood, spawning cup is placed in adult mosquito rearing cage and treats that female mosquito is laid eggs for the second time；
The mosquito ovum in the spawning cup of second of spawning is collected two days later.
Preferably, the blood of feeding includes feeding anti-freezing sheep blood, and the temperature of the anti-freezing sheep blood is 35-40 DEG C, feeds blood every time
Time is 1-2 hours.
Preferably, mosquito egg hatching is comprised the following steps for the method for larva：
It will be fermented for the artemia hatching solution for hatching mosquito ovum, the artemia hatching solution after being fermented；The artemia hatching solution is by yeast
Peptone and water prepare the obtained aqueous solution, and the concentration of Yeast protein peptone is 0.7-1.5g/L in the aqueous solution；
Weigh mosquito ovum to add in hatching pipe, the artemia hatching solution added in the hatching pipe after the fermentation, seal up hatching pipe
Lid, shake up, stand hatching, hatching terminate after open hatching pipe lid, the larva hatched is transferred in rearing tray.
Preferably, the artemia hatching solution is to prepare the obtained aqueous solution by Yeast protein peptone and water, yeast in the aqueous solution
The concentration of peptone is 0.8-1.0g/L.
Preferably, the artemia hatching solution is to prepare the obtained aqueous solution by Yeast protein peptone and water, yeast in the aqueous solution
The concentration of peptone is 0.9g/L.
Preferably, the Yeast protein peptone is Angel Yeast peptone.
Preferably, the temperature of the fermentation is 25 DEG C~30 DEG C, and the time of the fermentation is 20-28 hours.
Preferably, the addition of the artemia hatching solution after the fermentation is the 50-80% for hatching pipe volume.
Preferably, the addition of the artemia hatching solution after the fermentation is the 60-70% for hatching pipe volume.
Preferably, the proportioning of the mosquito ovum and the artemia hatching solution after the fermentation is 1g：150-350mL.
Preferably, the temperature for standing hatching is 25 DEG C~30 DEG C, and the time for standing hatching is small for 0.8-1.2
When.
Preferably, the mass concentration of the D/W is 8-12%.
Preferably, the adult mosquito rearing cage is placed in adult mosquito receptacle, and the temperature of the adult mosquito receptacle is 26-28 DEG C,
Relative humidity is 65-85%.
The method for breeding of the aedes albopictus spawning female mosquito of the present invention has advantages below and beneficial effect：
The present inventor is found surprisingly that in its long-term experience accumulation and experimentation, raises aedes albopictus
During female mosquito of laying eggs, the sex ration of tumbler plays a key effect to the feeding effect of female mosquito, spawning rate and laying quality.With the present invention
Described tumbler sex ration, which carries out raising, can obtain the quality height of higher spawning rate and gained mosquito ovum, and the incubation rate of the larvaes is high
(especially when sex ration is 3:When 1, female mosquito egg laying amount highest), substantially increase the spawning efficiency of aedes albopictus.
Method for breeding provided by the invention can guarantee that aedes albopictus preferably egg laying amount and laying quality, can be used as a kind of mark
The method for breeding of standardization spawning female mosquito, powerful guarantee is provided for the large-scale production of the aedes albopictus of laboratory and factory, to solve
The problem of ovum deficiency being supplied in aedes albopictus hero mosquito release production process.
The aqueous solution of the Yeast protein peptone configuration of method for breeding preferred special ratios in mosquito egg hatching step of the present invention
As artemia hatching solution, it can significantly accelerate the hatching rate of aedes albopictus mosquito ovum, incubation rate of the active mosquito ovum in 1 hour is very
Height, larval growth synchronism are good.And this method to the seal of hatching pipe without strict demand, it is a certain amount of when being left in hatching pipe
Oxygen on the contrary can be advantageously in will not be because of anoxic in the larva short time that hatching effect, a certain amount of oxygen make to hatch
And it is dead, survival rate of larvae is high, and prerequisite is created for the large-scale standardized raising of further aedes albopictus.
Embodiment
The method for breeding for female mosquito of being laid eggs below in conjunction with specific embodiment to the aedes albopictus of the present invention does further details of
Explanation.
Embodiment 1
A kind of method for breeding for aedes albopictus spawning female mosquito that the present embodiment provides, comprises the following steps：
Sprout wings：It it is 26-28 DEG C in temperature, relative humidity is in 65-85% adult mosquito receptacle, as the lineae ablicantes shown in table 1
The male and female quantity of the female pupa of yellow-fever mosquito and aedes albopictus hero pupa by the tumbler of the aedes albopictus after male and female separation than being put into adult mosquito rearing cage
(specification:In 30*30*30cm), and the lid of the dress pupa box in adult mosquito rearing cage is opened, tumbler is turned into adult mosquito, sprout wings
Period to adult mosquito provides the D/W that mass concentration is 10%, and (method is：10% is placed in the top of adult mosquito rearing cage
The cotton soaked with D/W, ensure that cotton contains enough D/Ws, and every other day change once
Cotton containing D/W), tumbler all turns into adult mosquito after 2 days.Each ratio carries out three parallel samples respectively
Experiment.
Spawning：Tumbler was turned into after adult mosquito after 5 days completely, and to female mosquito first time feeding anti-freezing sheep blood, (sheep blood temperature is tieed up
37 DEG C or so are held, is easy to female mosquito to suck；It is 1-2h or so to feed the blood time every time), raised for the first time after hello blood after one day in adult mosquito
Support placement spawning cup in cage and treat that female mosquito is laid eggs for the first time；The mosquito ovum in the spawning cup of spawning for the first time is collected two days later, and to female
Mosquito carries out second of feeding anti-freezing sheep blood；After one day after second of hello blood, spawning cup is placed in adult mosquito rearing cage and treats female mosquito
Second of spawning；The mosquito ovum in the spawning cup of second of spawning is collected two days later, counts quantity of laying eggs twice, as shown in table 1.
Hatching：Mosquito ovum collecting can reach maturity after one week, be larva by the mosquito egg hatching after maturation, and method is：Take ferment
Female peptone adds water to be configured to the aqueous solution that concentration is 0.9g/L, as artemia hatching solution；Used yeast peptone is Angel Yeast egg
White peptone FP101, the product are by the high protein bread yeast of pure culture, are handled through separation and concentration Yeast protein and complex enzyme is made
With a kind of obtained stable, safety and the comprehensive peptone of nutrition.Its content is as follows, total nitrogen：>=10.0%, a- ammonia nitrogen：≥
2.5%, moisture：≤ 6.0%, NaCl：≤ 2.0%, ash content：≤ 15.0%, pH (2% solution)：5.3-7.2；
Fermented 24 hours at a temperature of artemia hatching solution is positioned over into 25 DEG C~30 DEG C, the artemia hatching solution after being fermented；
Weigh 0.05g aedes albopictus mosquitos ovum (ten thousand ovum of 1g=15) to add in 15mL hatching pipes, added in hatching pipe
Artemia hatching solution after 10mL fermentations (artemia hatching solution after fermentation accounts for the 2/3 of hatching pipe volume)；The lid of hatching pipe is sealed up, is shaken up,
Hatching 1 hour is stood at a temperature of 25 DEG C~30 DEG C, the lid of hatching pipe is then opened, the larva hatched is transferred to raising
In disk.The incubation rate of the larvaes of mosquito ovum is counted, as shown in table 1.Wherein, the incubation rate of the larvaes refers to the larva number hatched and hatching institute
The ratio of mosquito ovum sum, incubation rate is higher, illustrates that the quality of mosquito ovum is higher, and the ratio of active mosquito ovum therein is higher.It is living
Property mosquito egg hatching rate refer to the ratio of the larva number that the hatches active mosquito ovum sum used with hatching.
Table 1
As the result of table 1 can be seen that：The sex ration of tumbler plays crucial work to female mosquito spawning rate and laying quality during raising
With.When sex ration is 2:1 to 4:When 1, single female mosquito eggs on average amount is 57.15~54.98, and larva average percentage hatch rate is
68.58%~71.90%, especially when sex ration is 3:When 1, female mosquito egg laying amount highest, and mosquito archiblast amount is high, the incubation rate of the larvaes
It is high；And when female-male proportion exceeds 1:1~5:During 1 this scope, its egg laying amount or incubation rate substantially reduce, when sex ration is 1:
When 2, single female mosquito eggs on average amount is 42.32；When sex ration is 6:When 1, single female mosquito eggs on average amount is 40.13,
Larva average percentage hatch rate is 53.43%.It can be seen that the preferred scope of sex ration is 2:1 to 4:1, sex ration is controlled in the scope
It is interior to obtain higher spawning rate and the incubation rate of the larvaes simultaneously.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that come for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. the method for breeding of a kind of aedes albopictus spawning female mosquito, it is characterised in that the step of including sprouting wings：By the mosquito of aedes albopictus
Pupa is put into adult mosquito rearing cage, tumbler is turned into adult mosquito, and the female pupa of aedes albopictus and lineae ablicantes are put into each adult mosquito rearing cage
The quantity ratio of yellow-fever mosquito hero pupa is 1~5:1.
2. the method for breeding of aedes albopictus spawning female mosquito according to claim 1, it is characterised in that raised in each adult mosquito
The quantity ratio that the female pupa of aedes albopictus and aedes albopictus hero pupa are put into cage is 2~4:1.
3. the method for breeding of aedes albopictus spawning female mosquito according to claim 2, it is characterised in that raised in each adult mosquito
The quantity ratio that the female pupa of aedes albopictus and aedes albopictus hero pupa are put into cage is 2.8~3.2:1.
4. the method for breeding of aedes albopictus spawning female mosquito according to claim 3, it is characterised in that raised in each adult mosquito
The quantity ratio that the female pupa of aedes albopictus and aedes albopictus hero pupa are put into cage is 3:1.
5. the method for breeding of the spawning female mosquito of the aedes albopictus according to claim any one of 1-4, it is characterised in that including
Following steps：
Sprout wings：The quantity ratio of the female pupa of aedes albopictus and aedes albopictus hero pupa as described in claim any one of 1-4 by lineae ablicantes she
The tumbler of mosquito is put into adult mosquito rearing cage, tumbler is turned into adult mosquito, and D/W is provided to adult mosquito during emergence, is sprouted wings
Time is 2-3 days；
Spawning：The tumbler turned into after adult mosquito after 4-6 days completely, feeds blood to female mosquito, is put after feeding blood in adult mosquito rearing cage
Put spawning cup and treat that female mosquito is laid eggs, the mosquito ovum in spawning cup is collected after spawning；
Hatching：It is larva by mosquito egg hatching after mosquito egg development 6-8 days.
6. the method for breeding of the spawning female mosquito of aedes albopictus according to claim 5, it is characterised in that the spawning bag
Include：The tumbler turned into after adult mosquito after 4-6 days completely, carried out feeding blood for the first time to female mosquito；
For the first time after one day after hello blood, spawning cup is placed in adult mosquito rearing cage and treats that female mosquito is laid eggs for the first time；
The mosquito ovum in the spawning cup of spawning for the first time is collected two days later, and is carried out second to female mosquito and fed blood；
After one day after second of hello blood, spawning cup is placed in adult mosquito rearing cage and treats that female mosquito is laid eggs for the second time；
The mosquito ovum in the spawning cup of second of spawning is collected two days later.
7. the method for breeding of aedes albopictus spawning female mosquito according to claim 5, it is characterised in that the blood of feeding includes feeding
Anti-freezing sheep blood, the temperature of the anti-freezing sheep blood is 35-40 DEG C, and the time for feeding blood every time is 1-2 hours.
8. the method for breeding of the aedes albopictus spawning female mosquito according to claim any one of 5-7, it is characterised in that by mosquito ovum
Hatch and comprise the following steps for the method for larva：
It will be fermented for the artemia hatching solution for hatching mosquito ovum, the artemia hatching solution after being fermented；The artemia hatching solution is by Yeast protein
Peptone and water prepare the obtained aqueous solution, and the concentration of Yeast protein peptone is 0.7-1.5g/L in the aqueous solution；
Weigh mosquito ovum to add in hatching pipe, the artemia hatching solution added in the hatching pipe after the fermentation, seal up the pipe of hatching pipe
Lid, shakes up, and stands hatching, and hatching opens the lid of hatching pipe, the larva hatched is transferred in rearing tray after terminating.
9. the method for breeding of the aedes albopictus spawning female mosquito according to claim any one of 5-7, it is characterised in that the Portugal
The mass concentration of grape sugar aqueous solution is 8-12%.
10. the method for breeding of the aedes albopictus spawning female mosquito according to claim any one of 5-7, it is characterised in that described
Adult mosquito rearing cage is placed in adult mosquito receptacle, and the temperature of the adult mosquito receptacle is 26-28 DEG C, relative humidity 65-85%.
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| CN108094768B (en) * | 2017-12-07 | 2021-05-28 | 吉林农业大学 | Wiggler straw fermentation substrate and wiggler cultivation method thereof |
| CN113749056A (en) * | 2021-08-31 | 2021-12-07 | 广州威佰昆生物科技有限公司 | Culex spawning and hatching method |
| CN117941655A (en) * | 2024-03-13 | 2024-04-30 | 暨南大学 | Efficient breeding method for culex pallidum |
| CN117941655B (en) * | 2024-03-13 | 2024-05-31 | 暨南大学 | Efficient breeding method for culex pallidum |
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