CN116636463A - Cultivation method of sphagnum moss in warm land in high-altitude area - Google Patents
Cultivation method of sphagnum moss in warm land in high-altitude area Download PDFInfo
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- CN116636463A CN116636463A CN202310838300.6A CN202310838300A CN116636463A CN 116636463 A CN116636463 A CN 116636463A CN 202310838300 A CN202310838300 A CN 202310838300A CN 116636463 A CN116636463 A CN 116636463A
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- 241000736285 Sphagnum Species 0.000 title claims abstract description 65
- 238000012364 cultivation method Methods 0.000 title claims abstract description 21
- 239000002689 soil Substances 0.000 claims abstract description 40
- 238000005286 illumination Methods 0.000 claims abstract description 39
- 239000001963 growth medium Substances 0.000 claims abstract description 35
- 230000001954 sterilising effect Effects 0.000 claims abstract description 27
- 230000035755 proliferation Effects 0.000 claims abstract description 19
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims abstract description 18
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000006013 carbendazim Substances 0.000 claims abstract description 18
- 239000006228 supernatant Substances 0.000 claims abstract description 17
- 238000005507 spraying Methods 0.000 claims abstract description 13
- 238000002834 transmittance Methods 0.000 claims abstract description 12
- MUKYLHIZBOASDM-UHFFFAOYSA-N 2-[carbamimidoyl(methyl)amino]acetic acid 2,3,4,5,6-pentahydroxyhexanoic acid Chemical compound NC(=N)N(C)CC(O)=O.OCC(O)C(O)C(O)C(O)C(O)=O MUKYLHIZBOASDM-UHFFFAOYSA-N 0.000 claims abstract description 6
- 241000159213 Zygophyllaceae Species 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims abstract description 6
- 235000009165 saligot Nutrition 0.000 claims abstract description 6
- 229920001817 Agar Polymers 0.000 claims description 22
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 22
- 229930006000 Sucrose Natural products 0.000 claims description 22
- 239000008272 agar Substances 0.000 claims description 22
- 239000000243 solution Substances 0.000 claims description 22
- 239000005720 sucrose Substances 0.000 claims description 22
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 19
- 238000004659 sterilization and disinfection Methods 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 12
- 239000004745 nonwoven fabric Substances 0.000 claims description 6
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 239000007943 implant Substances 0.000 claims 1
- 239000000047 product Substances 0.000 abstract description 13
- 239000003415 peat Substances 0.000 abstract description 9
- 230000008901 benefit Effects 0.000 abstract description 3
- 239000012773 agricultural material Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 5
- 241000195940 Bryophyta Species 0.000 description 4
- 230000008569 process Effects 0.000 description 3
- 239000003206 sterilizing agent Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
Abstract
The invention is applicable to the technical field of cultivation of sphagnum moss in warm areas, and provides a cultivation method of sphagnum moss in warm areas at high altitudes, which comprises the following steps: taking sphagnum caltrop spores as explants, sterilizing, inoculating the sphagnum caltrop spores into a primary culture medium, and culturing by illumination until protonema is formed on the explants; transferring the protonema on the explant into a proliferation culture medium, and carrying out illumination culture to obtain a large number of protonema; and (3) sterilizing a large number of protonema by using a carbendazim solution, spraying the protonema mixed IBA supernatant on soil, controlling the soil moisture to be 75-85%, building a shading net on the upper layer of the soil, and controlling the light transmittance of the shading net to be 40-50% and the concealment degree to be 50-60%. The invention can obviously improve the yield of moss, shorten the production and collection time of moss, scientifically control the investment cost of agricultural materials, shorten the planting period, promote the emergence rate of products, maximize the yield and the ratio benefit and greatly protect the wild resources of the peat moss in the warm fields.
Description
Technical Field
The invention belongs to the technical field of cultivation of sphagnum moss in warm fields, and particularly relates to a cultivation method of sphagnum moss in a warm field in a high-altitude area.
Background
Mosses are small multicellular green plants, currently about 2.3 tens of thousands of mosses are available worldwide, about 2800 kinds of mosses are available in China, and nowadays important ecological functions of mosses are increasingly receiving attention. However, along with the change of global climate, the forest area is gradually reduced, and the outdoor moss is used as landscape green vegetation to be continuously excavated, so that the diversity is also severely threatened. The market demand of moss is increasing at present, but the moss is only dug from wild, so that ecology is destroyed, and the moss is difficult to last, and moss cultivation is needed.
The current moss cultivation method is to collect wild peat moss for plant division and block cultivation, but the method has serious damage to wild resources, and the cultivation time period is long, and the method can reach the product sales standard (8-12 cm high) only by 2-3 years, thus being unfavorable for the development of industry, so that the method has important significance on how to cultivate the peat moss with high standard requirements in a short time.
Disclosure of Invention
The embodiment of the invention aims to provide a cultivation method of sphagnum moss in a high-altitude area, and aims to solve the problems in the background technology.
The embodiment of the invention is realized in such a way that the cultivation method of the sphagnum moss in the high-altitude area comprises the following steps:
taking sphagnum caltrop spores as explants, sterilizing, inoculating the sphagnum caltrop spores into a primary culture medium, and culturing by illumination until protonema is formed on the explants;
transferring the protonema on the explant into a proliferation culture medium, and carrying out illumination culture to obtain a large number of protonema;
and (3) sterilizing a large number of protonema by using a carbendazim solution, spraying the protonema mixed IBA supernatant on soil, controlling the soil moisture to be 75-85%, building a shading net on the upper layer of the soil, and controlling the light transmittance of the shading net to be 40-50% and the concealment degree to be 50-60%.
Preferably, the sphagnum calorium spores are used as explants, inoculated into a primary culture medium after disinfection, and subjected to light culture until a protonema is formed on the explants, wherein the disinfection specifically comprises the following steps:
placing the spores with seed shells removed into a non-woven fabric bag, placing the non-woven fabric bag filled with spores into ethanol water solution for first disinfection, and then placing into HgCl 2 The second disinfection is carried out in aqueous solution.
Preferably, the sphagnum calorium spores are used as explants, and inoculated into a primary culture medium after sterilization, and the primary culture medium comprises the following components in the steps of light culture until protonema are formed on the explants: 1/2MS+NAA0.2-0.5mg.L -1 +IAA0.1-0.5mg·L -1 +active carbon 1.0-2.0g.L -1 +sucrose 20-30 g.L -1 +agar 3-4 g.L -1 ，pH 5.0-5.8。
Preferably, the sphagnum calorium spores are used as explants, and inoculated into a primary culture medium after disinfection, and the process of light culture is carried out until protonema is formed on the explants, wherein the conditions of the light culture are as follows: the temperature is 22-28 ℃, and the illumination time is 10-15 h.d -1 The illumination intensity is 35-45 mu mol.m -2 ·s -1 。
Preferably, the step of transferring the protonema on the explant into a proliferation medium, and culturing by illumination to obtain a large number of protonema, wherein the proliferation medium comprises: MS+6-BA 0.2-1.0mg.L -1 +IBA0.02-0.1mg·L -1 +sucrose 20-30 g.L -1 +agar 3-4 g.L -1 ，pH 5.0-5.8。
Preferably, in the step of transferring the protonema on the explant into the proliferation medium and culturing by illumination to obtain a large number of protonema, the condition of the illumination culture is as follows: the temperature is 22-28 ℃, and the illumination time is 10-15 h.d -1 The illumination intensity is 35-45 mu mol.m -2 ·s -1 。
Preferably, after a plurality of protonemas are disinfected by using a carbendazim solution, the concentration of the carbendazim solution is 0.1-0.5% in the step of spraying the supernatant of the mixed IBA of the protonemas on soil.
Preferably, after the sterilization of a large number of protonema with carbendazim solution, the step of spraying the protonema mixed with IBA supernatant on the soil has a concentration of 0.025-0.05%.
According to the cultivation method for the sphagnum moss in the warm-ground area, provided by the embodiment of the invention, a large number of protonema can be obtained in a short time by collecting the sporidium of the sphagnum moss and performing tissue culture propagation by utilizing spores, the sphagnum moss can be cultivated in the high-altitude areas such as the north of Guidong, the north of Guizhou and the like in a high-efficiency spraying mode, the sphagnum moss product with the high quality and the high yield of 10-12cm can be produced about 8 months, the yield of the sphagnum moss can be remarkably improved, the production and collection time of the sphagnum moss can be shortened, the investment cost of agricultural materials can be scientifically controlled, the planting period can be shortened, the emergence rate of the product can be promoted, the yield and the benefit can be maximized, and the wild resources of the sphagnum moss in the warm-ground can be greatly protected.
Drawings
FIG. 1 shows collected sphagnum moss spores according to example 1 of the present invention;
FIG. 2 is a primary culture medium provided in example 1 of the present invention;
FIG. 3 shows a large number of protonema obtained by proliferation culture according to example 1 of the present invention;
FIG. 4 is a schematic view of a cultivation mode (width 1.50m×length 8m; light transmittance 50% and concealing 50%) provided in example 1 of the present invention;
FIG. 5 shows a sphagnum moss product cultivated for 8 months according to example 1 of the present invention;
FIG. 6 is a schematic view of the cultivation mode provided in comparative example 1 of the present invention (width 1.50 m. Times.length 8m; no shade net is installed, and the concealment is 0);
FIG. 7 is a diagram of a sphagnum moss product of comparative example 1 cultivated for 14 months.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
A cultivation method of sphagnum moss in a high-altitude area comprises the following steps:
(1) Taking sphagnum calorium spores as explants, sterilizing, inoculating to primary culture medium (formula of 1/2MS+NAA0.2-0.5mg.L) -1 +IAA0.1-0.5mg·L -1 +active carbon 1.0-2.0g.L -1 +sucrose 20-30 g.L -1 +agar 3-4 g.L -1 In the pH 5.0-5.8), the light culture (at 22-28deg.C for 10-15 hr.d -1 The illumination intensity is 35-45 mu mol.m -2 ·s -1 ) Until a protonema is formed in the explant;
the specific steps of the disinfection are as follows: placing the spores with seed shells removed into a non-woven fabric bag, placing the non-woven fabric bag filled with spores into ethanol water solution for first disinfection, and then placing into HgCl 2 Performing secondary disinfection in the aqueous solution; the seed husking is to gently and completely strip the seed husks by a sterilized scalpel; the mass percentage of the ethanol in the ethanol water solution is 75 percent, the time for the first sterilization is 40-80 s, more preferably 45-80 s, and still more preferably 65s; the HgCl 2 HgCl in aqueous solution 2 The mass percentage of the sterilizing agent is 0.1 percent, the time for the second sterilization is 5-15 min, more preferably 8min; the sterilization process is preferably accompanied by continuous shaking to allow more thorough contact of the spores with the sterilizing agent; rinsing the sterilized spores with sterile water to remove sterilizing agent on the surfaces of the spores, and sucking water on the surfaces of the spores by using sterile filter paper;
(2) Transferring the obtained protonema into proliferation culture medium (formula: MS+6-BA 0.2-1.0mg.L) -1 +IBA0.02-0.1mg·L -1 +sucrose 20-30 g.L -1 +agar 3-4 g.L -1 In the pH 5.0-5.8), the light culture (at 22-28deg.C for 10-15 hr.d -1 The illumination intensity is 35-45 mu mol.m -2 ·s -1 ) Obtaining a large number of protonema;
(3) After the obtained protofilament is disinfected by 0.1-0.5% carbendazim solution, the protofilament is mixed with 0.025-0.05% IBA supernatant to be sprayed on the soil, the soil moisture is kept at 75-85%, the cultivation mode is that the width is 1.50m and the length is 8-10m, a layer of shading net (the light transmittance is 40-50% and the concealment is 50-60%) is arranged on the upper layer of the soil, and the high-standard warm-ground peat moss product can be produced after 8-9 months.
Specific implementations of the invention are described in detail below in connection with specific embodiments.
Example 1
A cultivation method of sphagnum moss in a high-altitude area comprises the following steps:
(1) The sphagnum moss spores are used as explants, the sphagnum moss spores are shown in figure 1, and are inoculated into a primary culture medium (formula: 1/2MS+NAA0.3mg.L after disinfection -1 +IAA0.3mg·L -1 +activated carbon 1.5 g.L -1 +sucrose 25 g.L -1 +agar 3.5 g.L -1 pH 5.0), the primary culture medium is subjected to light culture (temperature 25 ℃ C., light time 13 h.d) -1 The illumination intensity was 40. Mu. Mol.m -2 ·s -1 ) Until a protonema is formed in the explant;
(2) Transferring the obtained protonema into proliferation culture medium (formula: MS+6-BA0.6mg.L) -1 +IBA0.06mg·L -1 +sucrose 25 g.L -1 +agar 3.5 g.L -1 pH 5.0), the culture was carried out under light (at 25℃for 13 h.d) -1 The illumination intensity was 40. Mu. Mol.m -2 ·s -1 ) A large number of protonemas are obtained, as shown in figure 3;
(3) After the obtained protofilament is disinfected by 0.1% carbendazim solution, the protofilament is mixed with 0.05% IBA supernatant to be sprayed on soil, the soil moisture is kept to be 75%, the cultivation mode is that the width is 1.50m times the length is 8m, and a layer of shading net (the light transmittance is 50% and the concealment is 50%) is arranged on the upper layer of the soil, as shown in fig. 4;
after 8 months, a warm-ground peat moss product is obtained, as shown in fig. 5, and according to fig. 5, the height of the warm-ground peat moss can reach about 12 cm.
Example 2
A cultivation method of sphagnum moss in a high-altitude area comprises the following steps:
(1) Taking sphagnum calorium spores as explants, sterilizing, inoculating to primary culture medium (formula of 1/2MS+NAA0.2mg.L) -1 +IAA 0.1mg·L -1 +activated carbon 1.0g.L -1 +sucrose 20 g.L -1 +agar 3 g.L -1 pH 5.0), the culture was carried out under light (at a temperature of 22℃for a time of 10 h.d) -1 The illumination intensity was 35. Mu. Mol.m -2 ·s -1 ) Until a protonema is formed in the explant;
(2) Transferring the obtained protonema into proliferation culture medium (formula: MS+6-BA 0.2mg.L) -1 +IBA 0.02mg·L -1 +sucrose 20 g.L -1 +agar 3 g.L -1 pH 5.0), the culture was carried out under light (at a temperature of 22℃for a time of 10 h.d) -1 The illumination intensity was 35. Mu. Mol.m -2 ·s -1 ) Obtaining a large number of protonema;
(3) And (3) sterilizing the obtained protofilament by adopting a 0.3% carbendazim solution, mixing the protofilament with 0.025% IBA supernatant, spraying on soil, keeping the soil moisture at 80%, and building a layer of shading net on the upper layer of the soil, wherein the cultivation mode is that the width is 1.50m multiplied by the length is 9m, and the light transmittance is 40% and the concealment is 60%.
Example 3
A cultivation method of sphagnum moss in a high-altitude area comprises the following steps:
(1) Taking sphagnum calorium spores as explants, sterilizing, inoculating to primary culture medium (formula of 1/2MS+NAA 0.5mg.L) -1 +IAA 0.5mg·L -1 +activated carbon 2.0g.L -1 +sucrose 30 g.L -1 +agar 4 g.L -1 pH 5.8), the culture was carried out under light (at 28℃for 15 h.d) -1 The illumination intensity was 45. Mu. Mol.m -2 ·s -1 ) Until a protonema is formed in the explant;
(2) Transferring the obtained protonema into proliferation medium (formula: MS+6-BA1.0mg.L) -1 +IBA 0.1mg·L -1 +sucrose 30 g.L -1 +agar 4 g.L -1 pH 5.8), the culture was carried out under light (at 28℃for 15 h.d) -1 Light, lightThe irradiation intensity was 45. Mu. Mol.m -2 ·s -1 ) Obtaining a large number of protonema;
(3) And (3) sterilizing the obtained protofilament by adopting a 0.5% carbendazim solution, mixing the protofilament with 0.04% IBA supernatant, spraying on soil, keeping the soil moisture at 85%, and setting a layer of shading net on the upper layer of the soil, wherein the cultivation mode is that the width is 1.50m multiplied by the length is 10m, the light transmittance is 45%, and the concealment is 55%.
Example 4
A cultivation method of sphagnum moss in a high-altitude area comprises the following steps:
(1) Taking sphagnum calorium spores as explants, sterilizing, inoculating to primary culture medium (formula of 1/2MS+NAA 0.3mg.L) -1 +IAA 0.2mg·L -1 +activated carbon 1.2 g.L -1 +sucrose 22 g.L -1 +agar 3 g.L -1 pH 5.2), the culture was carried out under light (at 24℃for 11 h.d) -1 The illumination intensity was 36. Mu. Mol.m -2 ·s -1 ) Until a protonema is formed in the explant;
(2) Transferring the obtained protonema into proliferation culture medium (formula: MS+6-BA 0.4mg.L) -1 +IBA0.04mg·L -1 +sucrose 22 g.L -1 +agar 3 g.L -1 pH 5.2), the culture was carried out under light (at 24℃for 11 h.d) -1 The illumination intensity was 38. Mu. Mol.m -2 ·s -1 ) Obtaining a large number of protonema;
(3) And (3) sterilizing the obtained protofilament by adopting a 0.2% carbendazim solution, mixing the protofilament with 0.03% IBA supernatant, spraying on soil, keeping the soil moisture at 75%, and setting a layer of shading net on the upper layer of the soil, wherein the planting mode is that the width is 1.50m and the length is 8m, the light transmittance is 42%, and the concealment is 58%.
Example 5
A cultivation method of sphagnum moss in a high-altitude area comprises the following steps:
(1) Taking sphagnum calorium spores as explants, sterilizing, inoculating to primary culture medium (formula of 1/2MS+NAA0.4mg.L) -1 +IAA 0.3mg·L -1 +activated carbon 1.6g.L -1 +sucrose 26 g.L -1 +agar 4 g.L -1 ，pH 5.4) In the process, the culture is carried out by illumination (the temperature is 26 ℃ C., the illumination time is 12 h.d) -1 The illumination intensity was 38. Mu. Mol.m -2 ·s -1 ) Until a protonema is formed in the explant;
(2) Transferring the obtained protonema into proliferation culture medium (formula: MS+6-BA 0.8mg.L) -1 +IBA0.08mg·L -1 +sucrose 26 g.L -1 +agar 4 g.L -1 pH 5.4), the culture was carried out under light (at 26℃for 12 h.d) -1 The illumination intensity was 38. Mu. Mol.m -2 ·s -1 ) Obtaining a large number of protonema;
(3) And (3) sterilizing the obtained protofilament by adopting a 0.3% carbendazim solution, mixing the protofilament with 0.04% IBA supernatant, spraying on soil, keeping the soil moisture at 75%, and building a layer of shading net on the upper layer of the soil, wherein the cultivation mode is that the width is 1.50m multiplied by the length is 8m, and the light transmittance is 44% and the concealment is 56%.
Example 6
A cultivation method of sphagnum moss in a high-altitude area comprises the following steps:
(1) Taking sphagnum calorium spores as explants, sterilizing, inoculating to primary culture medium (formula of 1/2MS+NAA 0.4mg.L) -1 +IAA0.4mg·L -1 +activated carbon 1.8g.L -1 +sucrose 28 g.L -1 +agar 4 g.L -1 pH 5.6), the culture was carried out under light (at 28℃for 14 h.d) -1 The illumination intensity was 43. Mu. Mol.m -2 ·s -1 ) Until a protonema is formed in the explant;
(2) Transferring the obtained protonema into proliferation culture medium (formula: MS+6-BA 0.8mg.L) -1 +IBA0.08mg·L -1 +sucrose 28 g.L -1 +agar 4 g.L -1 pH 5.6), the culture was carried out under light (at 28℃for 14 h.d) -1 The illumination intensity was 43. Mu. Mol.m -2 ·s -1 ) Obtaining a large number of protonema;
(3) And (3) sterilizing the obtained protofilament by adopting a 0.4% carbendazim solution, mixing the protofilament with 0.04% IBA supernatant, spraying on soil, keeping the soil moisture at 75%, and setting a layer of shading net on the upper layer of the soil, wherein the light transmittance is 48% and the concealment is 52%.
Comparative example 1
A cultivation method of sphagnum moss in a high-altitude area comprises the following steps:
(1) Taking sphagnum calorium spores as explants, sterilizing, inoculating to primary culture medium (formula of 1/2MS+NAA 0.3mg.L) -1 +IAA 0.3mg·L -1 +activated carbon 1.5 g.L -1 +sucrose 25 g.L -1 +agar 3.5 g.L -1 pH 5.0), the culture was carried out under light (at 25℃for 13 h.d) -1 The illumination intensity was 40. Mu. Mol.m -2 ·s -1 ) Until a protonema is formed in the explant;
(2) Transferring the obtained protonema into proliferation culture medium (formula: MS+6-BA 0.6mg.L) -1 +IBA 0.06mg·L -1 +sucrose 25 g.L -1 +agar 3.5 g.L -1 pH 5.0), the culture was carried out under light (at 25℃for 13 h.d) -1 The illumination intensity was 40. Mu. Mol.m -2 ·s -1 ) Obtaining a large number of protonema;
(3) After the obtained protofilament is disinfected by 0.1% carbendazim solution, the protofilament is mixed with 0.05% IBA supernatant to be sprayed on soil, the soil moisture is kept at 60%, the cultivation mode is that the width is 1.50m multiplied by the length is 8m, a shading net is not arranged on the upper layer of the soil, and the concealment degree is 0, as shown in figure 6;
the result of obtaining the sphagnum moss product after 14 months is shown in fig. 7, and according to fig. 7, it can be seen that the sphagnum moss product has lower quality.
Comparative example 2
A cultivation method of sphagnum moss in a high-altitude area comprises the following steps:
(1) Taking sphagnum calorium spores as explants, sterilizing, inoculating to primary culture medium (formula of 1/2MS+NAA 0.3mg.L) -1 +IAA 0.3mg·L -1 +activated carbon 1.5 g.L -1 +sucrose 25 g.L -1 +agar 3.5 g.L -1 pH 5.0), the culture was carried out under light (at 25℃for 13 h.d) -1 The illumination intensity was 40. Mu. Mol.m -2 ·s -1 ) Until a protonema is formed in the explant;
(2) Transferring the obtained protonema into proliferation culture medium (formula: MS+6-BA 0.6mg.L) -1 +IBA0.06mg·L -1 +sucrose 25 g.L -1 +agar 3.5 g.L -1 pH 5.0), the culture was carried out under light (at 25℃for 13 h.d) -1 The illumination intensity was 40. Mu. Mol.m -2 ·s -1 ) Obtaining a large number of protonema;
(3) Sterilizing the obtained protofilament by adopting 0.1% carbendazim solution, mixing the protofilament with 0.05% IBA supernatant, spraying on soil, keeping the soil moisture at 60-75%, and arranging a layer of shading net on the upper layer of the soil, wherein the cultivation mode is 1.50m wide by 8m long, the light transmittance is 75%, and the concealment is 25%;
and obtaining a peat moss product with an average value of 10.8cm after 14-18 months.
Comparative example 3
A cultivation method of sphagnum moss in a high-altitude area comprises the following steps:
collecting wild warm-land sphagnum, and adopting a separated planting mode and a cultivation mode: 1.50m wide by 8m long; no shading net;
and obtaining a peat moss product with an average value of 10.6cm after 20-22 months.
In conclusion, the cultivation method provided by the embodiment of the invention can obtain a high-standard warm-ground peat moss product in a short time.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.
Claims (8)
1. The cultivation method of the sphagnum moss in the high-altitude area is characterized by comprising the following steps of:
taking sphagnum caltrop spores as explants, sterilizing, inoculating the sphagnum caltrop spores into a primary culture medium, and culturing by illumination until protonema is formed on the explants;
transferring the protonema on the explant into a proliferation culture medium, and carrying out illumination culture to obtain a large number of protonema;
and (3) sterilizing a large number of protonema by using a carbendazim solution, spraying the protonema mixed IBA supernatant on soil, controlling the soil moisture to be 75-85%, building a shading net on the upper layer of the soil, and controlling the light transmittance of the shading net to be 40-50% and the concealment degree to be 50-60%.
2. The method for cultivating sphagnum moss in high altitude area according to claim 1, wherein the sphagnum moss spores are used as explants, the sphagnum moss spores are inoculated into a primary culture medium after disinfection, and the sphagnum moss spores are cultivated by illumination until protonema is formed on the explants, and the disinfection specifically comprises the following steps:
placing the spores with seed shells removed into a non-woven fabric bag, placing the non-woven fabric bag filled with spores into ethanol water solution for first disinfection, and then placing into HgCl 2 The second disinfection is carried out in aqueous solution.
3. The method according to claim 1, wherein the sphagnum moss spores are used as explants, the sphagnum moss spores are sterilized and inoculated into a primary culture medium, and the sphagnum moss spores are cultured under light until protonema is formed on the explants, and the primary culture medium comprises: 1/2MS+NAA0.2-0.5mg.L -1 +IAA0.1-0.5mg·L -1 +active carbon 1.0-2.0g.L -1 +sucrose 20-30 g.L -1 +agar 3-4 g.L -1 ，pH 5.0-5.8。
4. The method according to claim 1, wherein the sphagnum moss spores are used as explants, the sphagnum moss spores are inoculated into a primary culture medium after disinfection, and the sphagnum moss spores are subjected to light culture until protonema are formed on the explants, wherein the light culture conditions are as follows: the temperature is 22-28 ℃, and the illumination time is 10-15 h.d -1 The illumination intensity is 35-45 mu mol.m -2 ·s -1 。
5. The cultivation method of sphagnum moss in high altitude area according to claim 1, wherein the sphagnum moss is cultivated in the outsideTransferring the protonema on the implant into a proliferation culture medium, and carrying out light culture to obtain a large number of protonema, wherein the proliferation culture medium comprises the following components: MS+6-BA 0.2-1.0mg.L -1 +IBA0.02-0.1mg·L -1 +sucrose 20-30 g.L -1 +agar 3-4 g.L -1 ，pH 5.0-5.8。
6. The method for cultivating sphagnum moss in high altitude area according to claim 1, wherein, in the step of transferring the protonema on the explant into the proliferation medium, and culturing by illumination, the condition of the illumination culture is: the temperature is 22-28 ℃, and the illumination time is 10-15 h.d -1 The illumination intensity is 35-45 mu mol.m -2 ·s -1 。
7. The method for cultivating sphagnum moss in warm area at high altitude area according to claim 1, wherein after sterilizing a large number of protonema by using carbendazim solution, the protonema is mixed with IBA supernatant and sprayed on soil, the concentration of the carbendazim solution is 0.1-0.5%.
8. The method of claim 1, wherein the concentration of IBA supernatant is 0.025-0.05% in the step of spraying IBA supernatant onto soil after sterilizing a plurality of protonema with carbendazim solution.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104303759A (en) * | 2014-09-30 | 2015-01-28 | 贵州高原农产资材开发有限公司 | Soilless high-yield cultivation method of sphagnum junghuhnianum |
| CN105981636A (en) * | 2015-02-11 | 2016-10-05 | 上海市园林科学研究所 | Peat moss protonema rapid reproduction method |
| CN109089882A (en) * | 2018-08-30 | 2018-12-28 | 云南省农业科学院花卉研究所 | A kind of moss tissue culture directly induced by spore and seedling culture method |
| US20240008427A1 (en) * | 2020-11-24 | 2024-01-11 | Micropropagation Services (E.M.) Limited | Methods of in vitro culturing sphagnum |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104303759A (en) * | 2014-09-30 | 2015-01-28 | 贵州高原农产资材开发有限公司 | Soilless high-yield cultivation method of sphagnum junghuhnianum |
| CN105981636A (en) * | 2015-02-11 | 2016-10-05 | 上海市园林科学研究所 | Peat moss protonema rapid reproduction method |
| CN109089882A (en) * | 2018-08-30 | 2018-12-28 | 云南省农业科学院花卉研究所 | A kind of moss tissue culture directly induced by spore and seedling culture method |
| US20240008427A1 (en) * | 2020-11-24 | 2024-01-11 | Micropropagation Services (E.M.) Limited | Methods of in vitro culturing sphagnum |
Non-Patent Citations (2)
| Title |
|---|
| WENQIAN ZHAO等: "Development of a method for protonema proliferation of peat moss (Sphagnum squarrosum) through regeneration analysis", 《NEW PHYTOLOGIST》, vol. 221, 26 August 2018 (2018-08-26), pages 1160 - 1171, XP055893856, DOI: 10.1111/nph.15394 * |
| 姜山;田学武;: "药用苔藓植物银叶真藓和泥炭藓组织培养基配方研究", 西南大学学报(自然科学版), vol. 34, no. 12, 20 December 2012 (2012-12-20), pages 61 - 67 * |
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